Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F98 I0 R1 799 294.1 9752082 92.2% 8991419 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 648,056 C→A 63.6% F122L (TTC→TTA)  citA → sensory histidine kinase in two‑component regulatory system with CitB

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273648,0560CA63.6% 188.2 / 312.3 234F122L (TTC→TTA) citAsensory histidine kinase in two‑component regulatory system with CitB
Reads supporting (aligned to +/- strand):  ref base C (42/43);  new base A (82/67);  total (124/110)
Fisher's exact test for biased strand distribution p-value = 4.17e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.42e-01

Only 100 of 234 total aligned reads displayed.

GGTGAAAACGCGTGACTACAAACGGCTGGCGACCATCGCTAACAAATTACAAAGAGATACCGATTTTGATTATGTGGTGATTGGGGACCGGCACTCGATCCGCCTTTACCATCCTAATCCGGAGAAAATTGGTTATCCTATGCAGTTCACCAAACAGGGCGCGCTGGAGAAAGGGGAGAGCTACTTCATTACCGGGAAAGGGTCAATGGGGATGGCGATGCGCGCCAAAACGCCAATCTTTGATGACGATGGAAAAGTCATCGGCGTGGTGTCGATTGGCTACCTGGTGAGTAAA  >  CP009273/647909‑648203
                                                                                                                                                   |                                                                                                                                                   
ggTGAAAACGCGTGACTACAAACGGCTGGCGACCATCGCTAACAAATTACAAAGAGATACCGATTTTGATTATGTGGTGATTGGGGACCGGCACTCGATCCGCCTTTACCATCCTAATCCGGAGAAAATTGGTTATCCTATGCAGTTCAc                                                                                                                                                   <  1:1828016/150‑1 (MQ=255)
ggTGAAAACGCGTGACTACAAACGGCTGGCGACCATCGCTAACAAATTACAAAGAGATACCGATTTTGATTATGTGGTGATTGGGGACCGGCACTCGATCCGCCTTTACCATCCTAATCCGGAGAAAATTGGTTATCCTATGCAGTTAAc                                                                                                                                                   <  2:4406587/150‑1 (MQ=255)
  tGAAAACGCGTGACTACAAACGGCTGGCGACCATCGCTAACAAATTACAAAGAGATACCGATTTTGATTATGTGGTGATTGGGGACCGGCACTCGATCCGCCTTTACCATCCTAATCCGGAGAAAATTGGTTATCCTATGCAGTTAACCa                                                                                                                                                 <  1:4206965/150‑1 (MQ=255)
      aaCGCGTGACTACAAACGGCTGGCGACCATCGCTAACAAATTACAAAGAGATACCAATTTTGATTATGTGGTGATTGGGGACCGGCACTCGATCCGCCTTTACCATCCTAATCCGGAGAAAATTGGTTATCCTATGCAGTTAACCAAACa                                                                                                                                             <  1:3452922/150‑1 (MQ=255)
        cgcgTGACTACAAACGGCTGGCGACCATCGCTAACAAATTACAAAGAGATACCGATTTTGATTATGTGGTGATTGGGGACCGGCACTCGATCCGCCTTTACCATCCTAATCCGGAGAAAATTGGTTATCCTATGCAGTTCACCAAACAgg                                                                                                                                           >  1:3640909/1‑150 (MQ=255)
           gTGACTACAAACGGCTGGCGACCATCGCTAACAAATTACAAAGAGATACCGATTTTGATTATGTGGTGATTGGGGACCGGCACTCGATCCGCCTTTACCATCCTAATCCGGAGAAAATTGGTTATCCTATGCAGTTAACCAAACAGGgcg                                                                                                                                        >  2:4535416/1‑150 (MQ=255)
           gTGACTACAAACGGCTGGCGACCATCGCTAACAAATTACAAAGAGATACCGATTTTGATTATGTGGTGATTGGGGACCGGCACTCGATCCGCCTTTACCATCCTAATCCGGAGAAAATTGGTTATCCTATGCAGTTAACCAAACAGGgcg                                                                                                                                        >  1:3345189/1‑150 (MQ=255)
              aCTACAAACGGCTGGCGACCATCGCTAACAAATTACAAAGAGATACCGATTTTGATTATGTGGTGATTGGGGACCGGCACTCGATCCGCCTTTACCATCCTAATCCGGAGAAAATTGGTTATCCTATGCAGTTCACCAAACAGGgcgcgc                                                                                                                                     >  2:2645810/1‑150 (MQ=255)
                tACAAACGGCTGGCGACCATCGCTAACAAATTACAAAGAGATACCGATTTTGATTATGTGGTGATTGGGGACCGGCACTCGATCCGCCTTTACCATCCTAATCCGGAGAAAATTGGTTATCCTATGCAGTTAACCAAACAGGGCGCGCTg                                                                                                                                   <  1:4790827/150‑1 (MQ=255)
                 aCAAACGGCTGGCGACCATCGCTAACAAATTACAAAGAGATACCGATTTTGATTATGTGGTGATTGGGGACCGGCACTCGATCCGCCTTTACCATCCTAATCCGGAGAAAATTGGTTATCCTATGCAGTTCACCAAACAGGGCGCGCTgg                                                                                                                                  <  2:4840603/150‑1 (MQ=255)
                 aCAAACGGCTGGCGACCATCGCTAACAAATTACAAAGAGATACCGATTTTGATTATGTGGTGATTGGGGACCGGCACTCGATCCGCCTTTACCATCCTAATCCGGAGAAAATTGGTTATCCTATGCAGTTAACCAAACAGGGCGCGCTgg                                                                                                                                  >  1:2448034/1‑150 (MQ=255)
                  cAAACGGCTGGCGACCATCGCTAACAAATTACAAAGAGATACCGATTTTGATTATGTGGTGATTGGGGACCGGCACTCGATCCGCCTTTACCATCCTAATCCGGAGAAAATTGGTTATCCTATGCAGTTAACCAAACAGGGCGCGCTGga                                                                                                                                 >  2:3116803/1‑150 (MQ=255)
                     aCGGCTGGCGACCATCGCTAACAAATTACAAAGAGATACCGATTTTGATTATGTGGTGATTGGGGACCGGCACTCGATCCGCCTTTACCATCCTAATCCGGAGAAAATTGGTTATCCTATGCAGTTAACCAAACAGGGCGCGCTGGAGaa                                                                                                                              <  1:3683077/150‑1 (MQ=255)
                       ggCTGGCGACCATCGCTAACAAATTACAAAGAGATACCGATTTTGATTATGTGGTGATTGGGGACCGGCACTCGATCCGCCTTTACCATCCTAATCCGGAGAAAATTGGTTATCCTATGCAGTTCACCAAACAGGGCGCGCTGGAGAAAg                                                                                                                            <  2:1592946/150‑1 (MQ=255)
                       ggCTGGCGACCATCGCTAACAAATTACAAAGAGATACCGATTTTGATTATGTGGTGATTGGGGACCGGCACTCGATCCGCCTTTACCATCCTAATCCGGAGAAAATTGGTTATCCTATGCAGTTAACCAAACAGGGCGCGCTGGAGAAAg                                                                                                                            <  1:1078016/150‑1 (MQ=255)
                        gCTGGCGACCATCGCTAACAAATTACAAAGAGATACCGATTTTGATTATGTGGTGATTGGGGACCGGCACTCGATCCGCCTTTACCATCCTAATCCGGAGAAAATTGGTTATCCTATGCAGTTAACCAAACAGGGCGCGCTGGAGAAAgg                                                                                                                           <  2:4007742/150‑1 (MQ=255)
                         cTGGCGACCATCGCTAACAAATTACAAAGAGATACCGATTTTGATTATGTGGTGATTGGGGACCGGCACTCGATCCGCCTTTACCATCCTAATCCGGAGAAAATTGGTTATCCTATGCAGTTAACCAAACAGGGCGCGCTGGAGAAAggg                                                                                                                          <  2:872131/150‑1 (MQ=255)
                          tGGCGACCATCGCTAACAAATTACAAAGAGATACCGATTTTGATTATGTGGTGATTGGGGACCGGCACTCGATCCGCCTTTACCATCCTAATCCGGAGAAAATTGGTTATCCTATGCAGTTAACCAAACAGGGCGCGCTGGAGAAAgggg                                                                                                                         >  2:233899/1‑150 (MQ=255)
                          tGGCGACCATCGCTAACAAATTACAAAGAGATACCGATTTTGATTATGTGGTGATTGGGGACCGGCACTCGATCCGCCTTTACCATCCTAATCCGGAGAAAATTGGTTATCCTATGCAGTTAACCAAACAGGGCGCGCTGGAGAAAgggg                                                                                                                         >  2:3414999/1‑150 (MQ=255)
                                ccATCGCTAACAAATTACAAAGAGATACCGATTTTGATTATGTGGTGATTGGGGACCGGCACTCGATCCGCCTTTACCATCCTAATCCGGAGAAAATTGGTTATCCTATGCAGTTCACCAAACAGGGCGCGCTGGAGAAAGGGGAGAGCt                                                                                                                   <  1:2397156/150‑1 (MQ=255)
                                ccATCGCTAACAAATTACAAAGAGATACCGATTTTGATTATGTGGTGATTGGGGACCGGCACTCGATCCGCCTTTACCATCCTAATCCGGAGAAAATTGGTTATCCTATGCAGTTAACCAAACAGGGCGCGCTGGAGAAAGGGGAGAGCt                                                                                                                   <  1:4722381/150‑1 (MQ=255)
                                  aTCGCTAACAAATTACAAAGAGATACCGATTTTGATTATGTGGTGATTGGGGACCGGCACTCGATCCGCCTTTACCATCCTAATCCGGAGAAAATTGGTTATCCTATGCAGTTCACCAAACAGGGCGCGCTGGAGAAAGGGGAGAGCTAc                                                                                                                 >  1:2046894/1‑150 (MQ=255)
                                    cGCTAACAAATTACAAAGAGATACCGATTTTGATTATGTGGTGATTGGGGACCGGCACTCGATCCGCCTTTACCATCCTAATCCGGAGAAAATTGGTTATCCTATGCAGTTCACCAAACAGGGCGCGCTGGAGAAAGGGGAGAGCTACtt                                                                                                               <  2:4497644/150‑1 (MQ=255)
                                      cTAACAAATTACAAAGAGATACCGATTTTGATTATGTGGTGATTGGGGACCGGCACTCGATCCGCCTTTACCATCCTAATCCGGAGAAAATTGGTTATCCTATGCAGTTAACCAAACAGGGCGCGCTGGAGAAAGGGGAGAGCTACTTCa                                                                                                             >  1:1346660/1‑150 (MQ=255)
                                      cTAACAAATTACAAAGAGATACCGATTTTGATTATGTGGTGATTGGGGACCGGCACTCGATCCGCCTTTACCATCCTAATCCGGAGAAAATTGGTTATCCTATGCAGTTAACCAAACAGGGCGCGCTGGAGAAAGGGGAGAGCTACTTCa                                                                                                             >  2:2685883/1‑150 (MQ=255)
                                       tAACAAATTACAAAGAGATACCGATTTTGATTATGTGGTGATTGGGGACCGGCACTCGATCCGCCTTTACCATCCTAATCCGGAGAAAATTGGTTATCCTATGCAGTTCACCAAACAGGGCGCGCTGGAGAAAGGGGAGAGCTACTTCAt                                                                                                            >  2:4352132/1‑150 (MQ=255)
                                       tAACAAATTACAAAGAGATACCGATTTTGATTATGTGGTGATTGGGGACCGGCACTCGATCCGCCTTTACCATCCTAATCCGGAGAAAATTGGTTATCCTATGCAGTTCACCAAACAGGGCGCGCTGGAGAAAGGGGAGAGCTACTTCAt                                                                                                            <  1:4389749/150‑1 (MQ=255)
                                        aaCAAATTACAAAGAGATACCGATTTTGATTATGTGGTGATTGGGGACCGGCACTCGATCCGCCTTTACCATCCTAATCCGGAGAAAATTGGTTATCCTATGCAGTTAACCAAACAGGGCGCGCTGGAGAAAGGGGAGAGCTACTTCAtt                                                                                                           >  1:1885393/1‑150 (MQ=255)
                                        aaCAAATTACAAAGAGATACCGATTTTGATTATGTGGTGATTGGGGACCGGCACTCGATCCGCCTTTACCATCCTAATCCGGAGAAAATTGGTTATCCTATGCAGTTAACCAAACAGGGCGCGCTGGAGAAAGGGGAGAGCTACTTCAtt                                                                                                           <  1:1965440/150‑1 (MQ=255)
                                         aCAAATTACAAAGAGATACCGATTTTGATTATGTGGTGATTGGGGACCGGCACTCGATCCGCCTTTACCATCCTAATCCGGAGAAAATTGGTTATCCTATGCAGTTCACCAAACAGGGCGCGCTGGAGAAAGGGGAGAGCTACTTCATTa                                                                                                          <  2:3952292/150‑1 (MQ=255)
                                           aaaTTACAAAGAGATACCGATTTTGATTATGTGGTGATTGGGGACCGGCACTCGATCCGCCTTTACCATCCTAATCCGGAGAAAATTGGTTATCCTATGCAGTTAACCAAACAGGGCGCGCTGGAGAAAGGGGAGAGCTACTTCATTAcc                                                                                                        >  2:2531772/1‑150 (MQ=255)
                                            aaTTACAAAGAGATACCGATTTTGATTATGTGGTGATTGGGGACCGGCACTCGATCCGCCTTTACCATCCTAATCCGGAGAAAATTGGTTATCCTATGCAGTTCACCAAACAGGGCGCGCTGGAGAAAGGGGAGAGCTACTTCATTACCg                                                                                                       <  1:69765/150‑1 (MQ=255)
                                            aaTTACAAAGAGATACCGATTTTGATTATGTGGTGATTGGGGACCGGCACTCGATCCGCCTTTACCATCCTAATCCGGAGAAAATTGGTTATCCTATGCAGTTCACCAAACAGGGCGCGCTGGAGAAAGGGGAGAGCTACTTCATTACCg                                                                                                       <  1:2496780/150‑1 (MQ=255)
                                            aaTTACAAAGAGATACCGATTTTGATTATGTGGTGATTGGGGACCGGCACTCGATCCGCCTTTACCATCCTAATCCGGAGAAAATTGGTTATCCTATGCAGTTAACCAAACAGGGCGCGCTGGAGAAAGGGGAGAGCTACTTCATTACCg                                                                                                       >  2:2310171/1‑150 (MQ=255)
                                             aTTACAAAGAGATACCGATTTTGATTATGTGGTGATTGGGGACCGGCACTCGATCCGCCTTTACCATCCTAATCCGGAGAAAATTGGTTATCCTATGCAGTTCACCAAACAGGGCGCGCTGGAGAAAGGGGAGAGCTACTTCATTACCgg                                                                                                      >  1:3914107/1‑150 (MQ=255)
                                              ttACAAAGAGATACCGATTTTGATTATGTGGTGATTGGGGACCGGCACTCGATCCGCCTTTACCATCCTAATCCGGAGAAAATTGGTTATCCTATGCAGTTAACCAAACAGGGCGCGCTGGAGAAAGGGGAGAGCTACTTCATTACCggg                                                                                                     >  1:3589608/1‑150 (MQ=255)
                                              ttACAAAGAGATACCGATTTTGATTATGTGGTGATTGGGGACCGGCACTCGATCCGCCTTTACCATCCTAATCCGGAGAAAATTGGTTATCCTATGCAGTTAACCAAACAGGGCGCGCTGGAGAAAGGGGAGAGCTACTTCATTACCggg                                                                                                     >  1:4342665/1‑150 (MQ=255)
                                               tACAAAGAGATACCGATTTTGATTATGTGGTGATTGGGGACCGGCACTCGATCCGCCTTTACCATCCTAATCCGGAGAAAATTGGTTATCCTATGCAGTTAACCAAACAGGGCGCGCTGGAGAAAGGGGAGAGCTACTTCATTACCGGGa                                                                                                    <  2:3181646/150‑1 (MQ=255)
                                               tACAAAGAGATACCGATTTTGATTATGTGGTGATTGGGGACCGGCACTCGATCCGCCTTTACCATCCTAATCCGGAGAAAATTGGTTATCCTATGCAGTTAACCAAACAGGGCGCGCTGGAGAAAGGGGAGAGCTACTTCATTACCGGGa                                                                                                    <  2:3932054/150‑1 (MQ=255)
                                                aCAAAGAGATACCGATTTTGATTATGTGGTGATTGGGGACCGGCACTCGATCCGCCTTTACCATCCTAATCCGGAGAAAATTGGTTATCCTATGCAGTTAACCAAACAGGGCGCGCTGGAGAAAGGGGAGAGCTACTTCATTACCGGGaa                                                                                                   >  2:1721304/1‑150 (MQ=255)
                                                aCAAAGAGATACCGATTTTGATTATGTGGTGATTGGGGACCGGCACTCGATCCGCCTTTACCATCCTAATCCGGAGAAAATTGGTTATCCTATGCAGTTAACCAAACAGGGCGCGCTGGAGAAAGGGGAGAGCTACTTCATTACCGGGaa                                                                                                   <  1:1946903/150‑1 (MQ=255)
                                                 cAAAGAGATACCGATTTTGATTATGTGGTGATTGGGGACCGGCACTCGATCCGCCTTTACCATCCTCATCCGGAGAAAATTGGTTATCCTATGCAGTTAACCAAACAGGGCGCGCTGGAGAAAGGGGAGAGCTACTTCATTACCGGGaaa                                                                                                  >  2:4296546/1‑150 (MQ=255)
                                                  aaaGAGATACCGATTTTGATTATGTGGTGATTGGGGACCGGCACTCGATCCGCCTTTACCATCCTAATCCGGAGAAAATTGGTTATCCTATGCAGTTAACCAAACAGGGCGCGCTGGAGAAAGGGGAGAGCTACTTCATTACCGGGAAAg                                                                                                 >  2:3003255/1‑150 (MQ=255)
                                                   aaGAGATACCGATTTTGATTATGTGGTGATTGGGGACCGGCACTCGATCCGCCTTTACCATCCTAATCCGGAGAAAATTGGTTATCCTATGCAGTTAACCAAACAGGGCGCGCTGGAGAAAGGGGAGAGCTACTTCATTACCGGGAAAgg                                                                                                >  1:626815/1‑150 (MQ=255)
                                                   aaGAGATACCGATTTTGATTATGTGGTGATTGGGGACCGGCACTCGATCCGCCTTTACCATCCTAATCCGGAGAAAATTGGTTATCCTATGCAGTTAACCAAACAGGGCGCGCTGGAGAAAGGGGAGAGCTACTTCATTACCGGGAAAgg                                                                                                <  1:77158/150‑1 (MQ=255)
                                                    agagATACCGATTTTGATTATGTGGTGATTGGGGACCGGCACTCGATCCGCCTTTACCATCCTAATCCGGAGAAAATTGGTTATCCTATGCAGTTAACCAAACAGGGCGCGCTGGAGAAAGGGGAGAGCTACTTCATTACCGGGAAAggg                                                                                               <  1:40160/150‑1 (MQ=255)
                                                    agagATACCGATTTTGATTATGTGGTGATTGGGGACCGGCACTCGATCCGCCTTTACCATCCTAATCCGGAGAAAATTGGTTATCCTATGCAGTTAACCAAACAGGGCGCGCTGGAGAAAGGGGAGAGCTACTTCATTACCGGGAAAggg                                                                                               >  2:603652/1‑150 (MQ=255)
                                                          aCCGATTTTGATTATGTGGTGATTGGGGACCGGCACTCGATCCGCCTTTACCATCCTAATCCGGAGAAAATTGGTTATCCTATGCAGTTAACCAAACAGGGCGCGCTGGAGAAAGGGGAGAGCTACTTCATTACCGGGAAAGGGTCAATg                                                                                         >  1:4676012/1‑150 (MQ=255)
                                                             gATTTTGATTATGTGGTGATTGGGGACCGGCACTCGATCCGCCTTTACCATCCTAATCCGGAGAAAATTGGTTATCCTATGCAGTTAACCAAACAGGGCGCGCTGGAGAAAGGGGAGAGCTACTTCATTACCGGGAAAGGGTCAATgggg                                                                                      <  1:3486980/150‑1 (MQ=255)
                                                              aTTTTGATTATGTGGTGATTGGGGACCGGCACTCGATCCGCCTTTACCATCCTAATCCGGAGAAAATTGGTTATCCTATGCAGTTAACCAAACAGGGCGCGCTGGAGAAAGGGGAGAGCTACTTCATTACCGGGAAAGGGTCAATGGGGa                                                                                     >  1:2810106/1‑150 (MQ=255)
                                                               ttttGATTATGTGGTGATTGGGGACCGGCACTCGATCCGCCTTTACCATCCTAATCCGGAGAAAATTGGTTATCCTATGCAGTTCACCAAACAGGGCGCGCTGGAGAAAGGGGAGAGCTACTTCATTACCGGGAAAGGGTCAATGGGGAt                                                                                    >  1:2146893/1‑150 (MQ=255)
                                                                tttGATTATGTGGTGATTGGGGACCGGCACTCGATCCGCCTTTACCATCCTAATCCGGAGAAAATTGGTTATCCTATGCAGTTAACCAAACAGGGCGCGCTGGAGAAAGGGGAGAGCTACTTCATTACCGGGAAAGGGTCAATGGGGATg                                                                                   >  2:1031458/1‑150 (MQ=255)
                                                                 ttGATTATGTGGTGATTGGGGACCGGCACTCGATCCGCCTTTACCATCCTAATCCGGAGAAAATTGGTTATCCTATGCAGTTCACCAAACAGGGCGCGCTGGAGAAAGGGGAGAGCTACTTCATTACCGGGAAAGGGTCAATGGGGATgg                                                                                  <  2:2801033/150‑1 (MQ=255)
                                                                 ttGATTATGTGGTGATTGGGGACCGGCACTCGATCCGCCTTTACCATCCTAATCCGGAGAAAATTGGTTATCCTATGCAGTTAACCAAACAGGGCGCGCTGGAGAAAGGGGAGAGCTACTTCATTACCGGGAAAGGGTCAATGGGGATgg                                                                                  <  2:887611/150‑1 (MQ=255)
                                                                  tGATTATGTGGTGATTGGGGACCGGCACTCGATCCGCCTTTACCATCCTAATCCGGAGAAAATTGGTTATCCTATGCAGTTCACCAAACAGGGCGCGCTGGAGAAAGGGGAGAGCTACTTCATTACCGGGAAAGGGTCAATGGGGATGGc                                                                                 <  2:2509302/150‑1 (MQ=255)
                                                                   gATTATGTGGTGATTGGGGACCGGCACTCGATCCGCCTTTACCATCCTAATCCGGAGAAAATTGGTTATCCTATGCAGTTAACCAAACAGGGCGCGCTGGAGAAAGGGGAGAGCTACTTCATTACCGGGAAAGGGTCAATGGGGATGGCg                                                                                >  1:1796439/1‑150 (MQ=255)
                                                                     ttATGTGGTGATTGGGGACCGGCACTCGATCCGCCTTTACCATCCTAATCCGGAGAAAATTGGTTATCCTATGCAGTTAACCAAACAGGGCGCGCTGGAGAAAGGGGAGAGCTACTTCATTACCGGGAAAGGGTCAATGGGGATGGCGAt                                                                              >  2:586449/1‑150 (MQ=255)
                                                                        tgtgGTGATTGGGGACCGGCACTCGATCCGCCTTTACCATCCTAATCCGGAGAAAATTGGTTATCCTATGCAGTTAACCAAACAGGGCGCGCTGGAGAAAGGGGAGAGCTACTTCATTACCGGGAAAGGGTCAATGGGGATGGCGATgcg                                                                           >  1:1572231/1‑150 (MQ=255)
                                                                          tggtgATTGGGGACCGGCACTCGATCCGCCTTTACCATCCTAATCCGGAGAAAATTGGTTATCCTATGCAGTTAACCAAACAGGGCGCGCTGGAGAAAGGGGAGAGCTACTTCATTACCGGGAAAGGGTCAATGGGGATGGCGATgcgcg                                                                         >  2:4867726/1‑150 (MQ=255)
                                                                              gATTGGGGACCGGCACTCGATCCGCCTTTACCATCCTAATCCGGAGAAAATTGGTTATCCTATGCAGTTAACCAAACAGGGCGCGCTGGAGAAAGGGGAGAGCTACTTCATTACCGGGAAAGGGTCAATGGGGATGGCGATGCGCGCCaa                                                                     <  1:955309/150‑1 (MQ=255)
                                                                               aTTGGGGACCGGCACTCGATCCGCCTTTACCATCCTAATCCGGAGAAAATTGGTTATCCTATGCAGTTAACCAAACAGGGCGCGCTGGAGAAAGGGGAGAGCTACTTCATTACCGGGAAAGGGTCAATGGGGATGGCGATGCGCGCCaaa                                                                    >  1:140942/1‑150 (MQ=255)
                                                                                  ggggACCGGCACTCGATCCGCCTTTACCATCCTAATCCGGAGAAAATTGGTTATCCTATGCAGTTCACCAAACAGGGCGCGCTGGAGAAAGGGGAGAGCTACTTCATTACCGGGAAAGGGTCAATGGGGATGGCGATGCGCGCCAAAACg                                                                 >  2:4444239/1‑150 (MQ=255)
                                                                                     gACCGGCACTCGATCCGCCTTTACCATCCTAATCCGGAGAAAATTGGTTATCCTATGCAGTTAACCAAACAGGGCGCGCTGGAGAAAGGGGAGAGCTACTTCATTACCGGGAAAGGGTCAATGGGGATGGCGATGCGCGCCAAAACGCCa                                                              <  1:602361/150‑1 (MQ=255)
                                                                                      aCCGGCACTCGATCCGCCTTTACCATCCTAATCCGGAGAAAATTGGTTATCCTATGCAGTTCACCAAACAGGGCGCGCTGGAGAAAGGGGAGAGCTACTTCATTACCGGGAAAGGGTCAATGGGGATGGCGATGCGCGCCAAAACGCCaa                                                             >  1:795157/1‑150 (MQ=255)
                                                                                      aCCGGCACTCGATCCGCCTTTACCATCCTAATCCGGAGAAAATTGGTTATCCTATGCAGTTAACCAAACAGGGCGCGCTGGAGAAAGGGGAGAGCTACTTCATTACCGGGAAAGGGTCAATGGGGATGGCGATGCGCGCCAAAACGCCaa                                                             >  1:1926169/1‑150 (MQ=255)
                                                                                       ccGGAACTCGATCCGCCTTTACCATCATAATCCGGAGAAAATTGGTTATCCTATGCAGTTAACCAAACAGGGCGCGCTGGAGAAAGGGGAGAGCTACTTCATTACCGGGAAAGGGTCAATGGGGATGGCGATGCGCGCCAAAACGCCAAt                                                            <  2:4679549/150‑1 (MQ=255)
                                                                                        cGGCACTCGATCCGCCTTTACCATCCTAATCCGGAGAAAATTGGTTATCCTATGCAGTTAACCAAACAGGGCGCGCTGGAGAAAGGGGAGAGCTACTTCATTACCGGGAAAGGGTCAATGGGGATGGCGATGCGCGCCAAAACGCCAATc                                                           <  2:1485818/150‑1 (MQ=255)
                                                                                          gCACTCGATCCGCCTTTACCATCCTAATCCGGAGAAAATTGGTTATCCTATGCAGTTAACCAAACAGGGCGCGCTGGAGAAAGGGGAGAGCTACTTCATTACCGGGAAAGGGTCAATGGGGATGGCGATGCGCGCCAAAACGCCAATCtt                                                         <  2:2413227/150‑1 (MQ=255)
                                                                                           cACTCGATCCGCCTTTACCATCCTAATCCGGAGAAAATTGGTTATCCTATGCAGTTCACCAAACAGGGCGCGCTGGAGAAAGGGGAGAGCTACTTCATTACCGGGAAAGGGTCAATGGGGATGGCGATGCGCGCCAAAACGCCAATCttt                                                        <  2:481319/150‑1 (MQ=255)
                                                                                           cACTCGATCCGCCTTTACCATCCTAATCCGGAGAAAATTGGTTATCCTATGCAGTTAACCAAACAGGGCGCGCTGGAGAAAGGGGAGAGCTACTTCATTACCGGGAAAGGGTCAATGGGGATGGCGATGCGCGCCAAAACGCCAATCttt                                                        >  1:239091/1‑150 (MQ=255)
                                                                                            aCTCGATCCGCCTTTACCATCCTAATCCGGAGAAAATTGGTTATCCTATGCAGTTCACCAAACAGGGCGCGCTGGAGAAAGGGGAGAGCTACTTCATTACCGGGAAAGGGTCAATGGGGATGGCGATGCGCGCCAAAACGCCAATCTTtg                                                       <  2:3839464/150‑1 (MQ=255)
                                                                                             cTCGATCCGCCTTTACCATCCTAATCCGGAGAAAATTGGTTATCCTATGCAGTTCACCAAACAGGGCGCGCTGGAGAAAGGGGAGAGCTACTTCATTACCGGGAAAGGGTCAATGGGGATGGCGATGCGCGCCAAAACGCCAATCTTtga                                                      >  2:216142/1‑150 (MQ=255)
                                                                                              tCGATCCGCCTTTACCATCCTAATCCGGAGAAAATTGGTTATCCTATGCAGTTAACCAAACAGGGCGCGCTGGAGAAAGGGGAGAGCTACTTCATTACCGGGAAAGGGTCAATGGGGATGGCGATGCGCGCCAAAACGCCAATCTTtgat                                                     <  1:3150010/150‑1 (MQ=255)
                                                                                                gATCCGCCTTTACCATCCTAATCCGGAGAAAATTGGTTATCCTATGCAGTTAACCAAACAGGGCGCGCTGGAGAAAGGGGAGAGCTACTTCATTACCGGGAAAGGGTCAATGGGGATGGCGATGCGCGCCAAAACGCCAATCTTtgatga                                                   >  2:4157574/1‑150 (MQ=255)
                                                                                                      ccTTGACCATCCTACTCCGGAGAAAATTGGTTATCCTATGCAGTTAACCAAACCGGGCGCGCTGGAGAAAGGGGAGAGCTACTTCATTACCGGGAAAGGGTCAATGGGGATGGCGATGCGCGCCAAAACGCCAATCTTTGATGACGATgg                                             <  2:4390504/150‑1 (MQ=255)
                                                                                                              aTCCTAATCCGGAGAAAATTGGTTATCCTATGCAGTTAACCAAACAGGGCGCGCTGGAGAAAGGGGAGAGCTACTTCATTACCGGGAAAGGGTCAATGGGGATGGCGATGCGCGCCAAAACGCCAATCTTTGATGACGATGGAAAAGTCa                                     >  2:399839/1‑150 (MQ=255)
                                                                                                                ccTAATCCGGAGAAAATTGGTTATCCTATGCAGTTCACCAAACAGGGCGCGCTGGAGAAAGGGGAGAGCTACTTCATTACCGGGAAAGGGTCAATGGGGATGGCGATGCGCGCCAAAACGCCAATCTTTGATGACGATGGAAAAGTCATc                                   >  1:4518117/1‑150 (MQ=255)
                                                                                                                ccTAATCCGGAGAAAATTGGTTATCCTATGCAGTTAACCAAACAGGGCGCGCTGGAGAAAGGGGAGAGCTACTTCATTACCGGGAAAGGGTCAATGGGGATGGCGATGCGCGCCAAAACGCCAATCTTTGATGACGATGGAAAAGTCATc                                   >  2:1955144/1‑150 (MQ=255)
                                                                                                                ccTAATCCGGAGAAAATTGGTTATCCTATGCAGTTAACCAAACAGGGCGCGCTGGAGAAAGGGGAGAGCTACTTCATTACCGGGAAAGGGTCAATGGGGATGGCGATGCGCGCCAAAACGCCAATCTTTGATGACGATGGAAAAGTCATc                                   >  2:1955028/1‑150 (MQ=255)
                                                                                                                   aaTCCGGAGAAAATTGGTTATCCTATGCAGTTAACCAAACAGGGCGCGCTGGAGAAAGGGGAGAGCTACTTCATTACCGGGAAAGGGTCAATGGGGATGGCGATGCGCGCCAAAACGCCAATCTTTGATGACGATGGAAAAGTCATCGGc                                <  1:3003255/150‑1 (MQ=255)
                                                                                                                     tCCGGAGAAAATTGGTTATCCTATGCAGTTAACCAAACAGGGCGCGCTGGAGAAAGGGGAGAGCTACTTCATTACCGGGAAAGGGTCAATGGGGATGGCGATGCGCGCCAAAACGCCAATCTTTGATGACGATGGAAAAGTCATCGGCgt                              <  2:1994202/150‑1 (MQ=255)
                                                                                                                      ccGGAGAAAATTGGTTATCCTATGCAGTTAACCAAACAGGGCGCGCTGGAGAAAGGGGAGAGCTACTTCATTACCGGGAAAGGGTCAATGGGGATGGCGATGCGCGCCAAAACGCCAATCTTTGATGACGATGGAAAAGTCATCGGCgtg                             <  2:3611059/150‑1 (MQ=255)
                                                                                                                        ggAGAAAATTGGTTATCCTATGCAGTTAACCAAACAGGGCGCGCTGGAGAAAGGGGAGAGCTACTTCATTACCGGGAAAGGGTCAATGGGGATGGCGATGCGCGCCAAAACGCCAATCTTTGATGACGATGGAAAAGTCATCGGCgtggt                           >  2:2244650/1‑150 (MQ=255)
                                                                                                                            aaaaTTGGTTATCCTATGCAGTTAACCAAACAGGGCGCGCTGGAGAAAGGGGAGAGCTACTTCATTACCGGGAAAGGGTCAATGGGGATGGCGATGCGCGCCAAAACGCCAATCTTTGATGACGATGGAAAAGTCATCGGCGTGGTGTCg                       >  2:1471864/1‑150 (MQ=255)
                                                                                                                              aaTTGGTTATCCTATGCAGTTAACCAAACAGGGCGCGCTGGAGAAAGGGGAGAGCTACTTCATTACCGGGAAAGGGTCAATGGGGATGGCGATGCGCGCCTAAACGCCAATCTTTGATGACGATGGAAAAGTCATCGGCGTGGTGTCGAt                     >  2:1667370/1‑150 (MQ=255)
                                                                                                                               aTTGGTTATCCTATGCAGTTAACCAAACAGGGCGCGCTGGAGAAAGGGGAGAGCTACTTCATTACCGGGAAAGGGTCAATGGGGATGGCGATGCGCGCCAAAACGCCAATCTTTGATGACGATGGAAAAGTCATCGGCGTGGTGTCGAtt                    <  1:3766610/150‑1 (MQ=255)
                                                                                                                                   gTTATCCTATGCAGTTAACCAAACAGGGCGCGCTGGAGAAAGGGGAGAGCTACTTCATTACCGGGAAAGGGTCAATGGGGATGGCGATGCGCGCCAAAACGCCAATCTTTGATGACGATGGAAAAGTCATCGGCGTGGTGTCGATTGGCt                >  2:2116864/1‑150 (MQ=255)
                                                                                                                                     tATCCTATGCAGTTAACCAAACAGGGCGCGCTGGAGAAAGGGGAGAGCTACTTCATTACCGGGAAAGGGTCAATGGGGATGGCGATGCGCGCCAAAACGCCAATCTTTGATGACGATGGAAAAGTCATCGGCGTGGTGTCGATTGGCTAc              >  2:1701817/1‑150 (MQ=255)
                                                                                                                                      aTCCTATGCAGTTCACCAAACAGGGCGCGCTGGAGAAAGGGGAGAGCTACTTCATTACCGGGAAAGGGTCAATGGGGATGGCGATGCGCGCCAAAACGCCAATCTTTGATGACGATGGAACAGTCATCGGCGTGGTGTCGATTGGCTAcc             >  2:729316/1‑150 (MQ=255)
                                                                                                                                      aTCCTATGCAGTTCACCAAACAGGGCGCGCTGGAGAAAGGGGAGAGCTACTTCATTACCGGGAAAGGGTCAATGGGGATGGCGATGCGCGCCAAAACGCCAATCTTTGATGACGATGGAAAAGTCATCGGCGTGGTGTCGATTGGCTAcc             <  1:2645810/150‑1 (MQ=255)
                                                                                                                                      aTCCTATGCAGTTCACCAAACAGGGCGCGCTGGAGAAAGGGGAGAGCTACTTCATTACCGGGAAAGGGTCAATGGGGATGGCGATGCGCGCCAAAACGCCAATCTTTGATGACGATGGAAAAGTCATCGGCGTGGTGTCGATTGGCTAcc             >  2:2100546/1‑150 (MQ=255)
                                                                                                                                      aTCCTATGCAGTTAACCAAACAGGGCGCGCTGGAGAAAGGGGAGAGCTACTTCATTACCGGGAAAGGGTCAATGGGGATGGCGATGCGCGCCAAAACGCCAATCTTTGATGACGATGGAAAAGTCATCGGCGTGGTGTCGATTGGCTAcc             >  1:226756/1‑150 (MQ=255)
                                                                                                                                       tCCTATGCAGTTAACCAAACAGGGCGCGCTGGAGAAAGGGGAGAGCTACTTCATTACCGGGAAAGGGTCAATGGGGATGGCGATGCGCGCCAAAACGCCAATCTTTGATGACGATGGAAAAGTCATCGGCGTGGTGTCGATTGGCTACCt            <  2:1346660/150‑1 (MQ=255)
                                                                                                                                        ccTATGCAGTTCACCAAACAGGGCGCGCTGGAGAAAGGGGAGAGCTACTTCATTACCGGGAAAGGGTCAATGGGGATGGCGATGCGCGCCAAAACGCCAATCTTTGATGACGATGGAAAAGTCATCGGCGTGGTGTCGATTGGCTACCTg           <  2:3914101/150‑1 (MQ=255)
                                                                                                                                           aTGCAGTTAACCAAACCGGGCGCGCTGGAGAAAGGGGAGAGCTACTTCATTACCGGGAAAGGGTCAATGGGGATGGCGATGCGCGCCAAAACGCCAATCTTTGATGACGATGGAAAAGTCATCGGCGTGGTGTCGATTGGCTACCTGGTg        <  1:3290873/150‑1 (MQ=255)
                                                                                                                                              cAGTTAACCAAACAGGGCGCGCTGGAGAAAGGGGAGAGCTACTTCATTACCGGGAAAGGGTCAATGGGGATGGTGATGCGCGCCAAAACGCCAATCTTTGATGACGATGGAAAAGTCATCGGCGTGGTGTCGATTGGCTACCTGGTGAGt     >  1:112728/1‑150 (MQ=255)
                                                                                                                                               aGTTCACCAAACAGGGCGCGCTGGAGAAAGGGGAGAGCTACTTCATTACCGGGAAAGGGTCAATGGGGATGGCGATGCGCGCCAAAACGCCAATCTTTGATGACGATGGAAAAGTCATCGGCGTGGTGTCGATTGGCTACCTGGTGAGTa    >  1:382328/1‑150 (MQ=255)
                                                                                                                                                gTTAACCAAACAGGGCGCGCTGGAGAAAGGGGAGAGCTACTTCATTACCGGGAAAGGGTCAATGGGGATGGCGATGCGCGCCAAAACGCCAATCTTTGATGACGATGGAAAAGTCATCGGCGTGGTGTCGATTGGCTACCTGGTGAGTaa   >  1:296299/1‑150 (MQ=255)
                                                                                                                                                gTTAACCAAACAGGGCGCGCTGGAGAAAGGGGAGAGCTACTTCATTACCGGGAAAGGGTCAATGGGGATGGCGATGCGCGCCAAAACGCCAATCTTTGATGACGATGGAAAAGTCATCGGCGTGGTGTCGATTGGCTACCTGGTGAGTaa   <  1:1031458/150‑1 (MQ=255)
                                                                                                                                                 ttCACCAAACAGGGCGCGCTGGAGAAAGGGGAGAGCTACTTCATTACCGGGAAAGGGTCAATGGGGATGGCGATGCGCGCCAAAACGCCAATCTTTGATGACGATGGAAAAGTCATCGGCGTGGTGTCGATTGGCTACCTGGTGAGTaaa  <  2:3640945/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                   
GGTGAAAACGCGTGACTACAAACGGCTGGCGACCATCGCTAACAAATTACAAAGAGATACCGATTTTGATTATGTGGTGATTGGGGACCGGCACTCGATCCGCCTTTACCATCCTAATCCGGAGAAAATTGGTTATCCTATGCAGTTCACCAAACAGGGCGCGCTGGAGAAAGGGGAGAGCTACTTCATTACCGGGAAAGGGTCAATGGGGATGGCGATGCGCGCCAAAACGCCAATCTTTGATGACGATGGAAAAGTCATCGGCGTGGTGTCGATTGGCTACCTGGTGAGTAAA  >  CP009273/647909‑648203

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GCGGTGAAAACGCGTGACTACAAACGGCTGGCGACCATCGCTAACAAATTACAAAGAGATACCGATTTTGATTATGTGGTGATTGGGGACCGGCACTCGATCCGCCTTTACCATCCTAATCCGGAGAAAATTGGTTATCCTATGCAGTTCACCAAACAGGGCGCGCTGGAGAAAGGGGAGAGCTACTTCATTACCGGGAAAGGGTCAATGGGGATGGCGATGCGCGCCAAAACGCCAATCTTTGATGACGATGGAAAAGTCATCGGCGTGGTGTCGATTGGCTACCTGGTGAGTAA  >  CP009273/647907‑648202
                                                                                                                                                     |                                                                                                                                                  
GCGGTGAAAACGCGTGACTACAAACGGCTGGCGACCATCGCTAACAAATTACAAAGAGATACCGATTTTGATTATGTGGTGATTGGGGACCGGCACTCGATCCGCCTTTACCATCCTAATCCGGAGAAAATTGGTTATCCTATGCAGTTA                                                                                                                                                    <  SRR19973081.194374/150‑1 (MQ=60)
 CGGTGAAAACGCGTGACTACAAACGGCTGGCGACCATCGCTAACAAATTACAAAGAGATACCGATTTTGATTATGTGGTGATTGGGGACCGGCACTCGATCCGCCTTTACCATCCTAATCCGGAGAAAATTGGTTATCCTATGCAGTTAA                                                                                                                                                   <  SRR19973081.1553919/150‑1 (MQ=60)
  GGTGAAAACGCGTGACTACAAACGGNTGGCGACCATCGCTAACAAATTACANAGAGATANCGATTTTGATTATGTNGNGATTGGGGACCGNCACTCGATCCGCCTTTACCATCCTAATCCGGAGAAAATTGGTTATCCTATGCAGTTAAC                                                                                                                                                  >  SRR19973081.401931/1‑150 (MQ=60)
  GGTGAAAACGCGTGACTACAAACGGCTGGCGACCATCGCTAACAAATTACAAAGAGATACCGATTTTGATTATGTGGTGATTGGGGACCGGCACTCGATCCGCCTTTACCATCCTAATCCGGAGAAAATTGGTTATCCTATGCAGTTCAC                                                                                                                                                  <  SRR19973081.1839918/150‑1 (MQ=60)
  GGTGAAAACGCGTGACTACAAACGGCTGGCGACCATCGCTAACAAATTACAAAGAGATACCGATTTTGATTATGTGGTGATTGGGGACCGGCACTCGATCCGCCTTTACCATCCTAATCCGGAGAAAATTGGTTATCCTATGCAGTTCAC                                                                                                                                                  <  SRR19973081.4305387/150‑1 (MQ=60)
   GTGAAAACGCGTGACTACAAACGGCTGGCGACCATCGCTAACAAATTACAAAGAGATACCGATTTTGATTATGTGGTGATTGGGGACCGGCACTCGATCCGCCTTTACCATCCTAATCCGGAGAAAATTGGTTATCCTATGCAGTTAACC                                                                                                                                                 <  SRR19973081.866998/150‑1 (MQ=60)
    TGAAAACGCGTGACTACAAACGGCTGGCGACCATCGCTAACAAATTACAAAGAGATACCGATTTTGATTATGTGGTGATTGGGGACCGGCACTCGATCCGCCTTTACCATCCTAATCCGGAGAAAATTGGTTATCCTATGCAGTTCACCA                                                                                                                                                <  SRR19973081.3476575/150‑1 (MQ=60)
    TGAAAACGCGTGACTACAAACGGCTGGCGACCATCGCTAACAAATTACAAAGAGATACCGATTTTGATTATGTGGTGATTGGGGACCGGCACTCGATCCGCCTTTACCATCCTAATCCGGAGAAAATTGGTTATCCTATGCAGTTAACCA                                                                                                                                                <  SRR19973081.4232670/150‑1 (MQ=60)
        AACGCGTGACTACAAACGGCTGGCGACCATCGCTAACAAATTACAAAGAGATACCAATTTTGATTATGTGGTGATTGGGGACCGGCACTCGATCCGCCTTTACCATCCTAATCCGGAGAAAATTGGTTATCCTATGCAGTTAACCAAACA                                                                                                                                            <  SRR19973081.3474158/150‑1 (MQ=60)
          CGCGTGACTACAAACGGCTGGCGACCATCGCTAACAAATTACAAAGAGATACCGATTTTGATTATGTGGTGATTGGGGACCGGCACTCGATCCGCCTTTACCATCCTAATCCGGAGAAAATTGGTTATCCTATGCAGTTCACCAAACAGG                                                                                                                                          >  SRR19973081.3662871/1‑150 (MQ=60)
          CGCGTGACTACAAACGGCTGGCGACCATCGCTAACAAATTACAAAGAGATACCGATTTTGATTATGTGGTGATTGGGGACCGGCACTCGATCCGCCTTTACCATCCTAATCCGGAGAAAATTGGTTATCCTATGCAGTTCACCAAACAGG                                                                                                                                          >  SRR19973081.3662907/1‑150 (MQ=60)
             GTGACTACAAACGGCTGGCGACCATCGCTAACAAATTACAAAGAGATACCGATTTTGATTATGTGGTGATTGGGGACCGGCACTCGATCCGCCTTTACCATCCTAATCCGGAGAAAATTGGTTATCCTATGCAGTTAACCAAACAGGGCG                                                                                                                                       >  SRR19973081.3365710/1‑150 (MQ=60)
                 CTACAAACGGCTGGCGACCATCGCTAACAAATTACAAAGAGATACCGATTTTGATTATGTGGTGATTGGGGACCGGCACTCGATCCGCCTTTACCATCCTAATCCGGAGAAAATTGGTTATCCTATGCAGTTAACCAAACAGGGCGCGCT                                                                                                                                   <  SRR19973081.1323772/150‑1 (MQ=60)
                  TACAAACGGCTGGCGACCATCGCTAACAAATTACAAAGAGATACCGATTTTGATTATGTGGTGATTGGGGACCGGCACTCGATCCGCCTTTACCATCCTAATCCGGAGAAAATTGGTTATCCTATGCAGTTAACCAAACAGGGCGCGCTG                                                                                                                                  <  SRR19973081.4819716/150‑1 (MQ=60)
                   ACAAACGGCTGGCGACCATCGCTAACAAATTACAAAGAGATACCGATTTTGATTATGTGGTGATTGGGGACCGGCACTCGATCCGCCTTTACCATCCTAATCCGGAGAAAATTGGTTATCCTATGCAGTTAACCAAACAGGGCGCGCTGG                                                                                                                                 >  SRR19973081.2463212/1‑150 (MQ=60)
                   ACAAACGGCTGGCGACCATCGCTAACAAATTACAAAGAGATACCGATTTTGATTATGTGGTGATTGGGGACCGGCACTCGATCCGCCTTTACCATCCTAATCCGGAGAAAATTGGTTATCCTATGCAGTTAACCAAACAGGGCGCGCTGG                                                                                                                                 >  SRR19973081.2463126/1‑150 (MQ=60)
                     AAACGGCTGGCGACCATCGCTAACAAATTACAAAGAGATACCGATTTTGATTATGTGGTGATTGGGGACCGGCACTCGATCCGCCTTTACCATCCTAATCCGGAGAAAATTGGTTATCCTATGCAGTTCACCAAACAGGGCGCGCTGGAG                                                                                                                               <  SRR19973081.4727510/150‑1 (MQ=60)
                       ACGGCTGGCGACCATCGCTAACAAATTACAAAGAGATACCGATTTTGATTATGTGGTGATTGGGGACCGGCACTCGATCCGCCTTTACCATCCTAATCCGGAGAAAATTGGTTATCCTATGCAGTTAACCAAACAGGGCGCGCTGGAGAA                                                                                                                             <  SRR19973081.3705426/150‑1 (MQ=60)
                         GGCTGGCGACCATCGCTAACAAATTACAAAGAGATACCGATTTTGATTATGTGGTGATTGGGGACCGGCACTCGATCCGCCTTTACCATCCTAATCCGGAGAAAATTGGTTATCCTATGCAGTTAACCAAACAGGGCGCGCTGGAGAAAG                                                                                                                           <  SRR19973081.1085208/150‑1 (MQ=60)
                         GGCTGGCGACCATCGCTAACAAATTACAAAGAGATACCGATTTTGATTATGTGGTGATTGGGGACCGGCACTCGATCCGCCTTTACCATCCTAATCCGGAGAAAATTGGTTATCCTATGCAGTTAACCAAACAGGGCGCGCTGGAGAAAG                                                                                                                           <  SRR19973081.1085172/150‑1 (MQ=60)
                            TGGCGACTATCGCTAACAAATTACAAAGAGATACCGATTTTGATTATGTGGTGATTGGGGACCGGCACTCGATCCGCCTTTACCATCCTAATCCGGAGAAAATTGGTTATCCTATGCAGTTCACCAAACAGGGCGCGCTGGAGAAAGGGG                                                                                                                        >  SRR19973081.985932/1‑150 (MQ=60)
                            TGGCGACCATCGCTAACAAATTACAAAGAGATACCGATTTTGATTATGTGGTGATTGGGGACCGGCACTCGATCCGCCTTTACCATCCTAATCCGGAGAAAATTGGTTATCCTATGCAGTTCACCAAACAGGGCGCGCTGGAGAAAGGGG                                                                                                                        >  SRR19973081.2292028/1‑150 (MQ=60)
                                  CCATCGCTAACAAATTACAAAGAGATACCGATTTTGATTATGTGGTGATTGGGGACCGGCACTCGATCCGCCTTTACCATCCTAATCCGGAGAAAATTGGTTATCCTATGCAGTTCACCAAACAGGGCGCGCTGGAGAAAGGGGAGAGCT                                                                                                                  <  SRR19973081.2411930/150‑1 (MQ=60)
                                  CCATCGCTAACAAATTACAAAGAGATACCGATTTTGATTATGTGGTGATTGGGGACCGGCACTCGATCCGCCTTTACCATCCTAATCCGGAGAAAATTGGTTATCCTATGCAGTTAACCAAACAGGGCGCGCTGGAGAAAGGGGAGAGCT                                                                                                                  <  SRR19973081.4750973/150‑1 (MQ=60)
                                  CCATCGCTAACAAATTACAAAGAGATACCGATTTTGATTATGTGGTGATTGGGGACCGGCACTCGATCCGCCTTTACCATCCTAATCCGGAGAAAATTGGTTATCCTATGCAGTTAACCAAACAGGGCGCGCTGGAGAAAGGGGAGAGCT                                                                                                                  <  SRR19973081.2286078/150‑1 (MQ=60)
                                    ATCGCTAACAAATTACAAAGAGATACCGATTTTGATTATGTGGTGATTGGGGACCGGCACTCGATCCGCCTTTACCATCCTAATCCGGAGAAAATTGGTTATCCTATGCAGTTCACCAAACAGGGCGCGCTGGAGAAAGGGGAGAGCTAC                                                                                                                >  SRR19973081.2059783/1‑150 (MQ=60)
                                        CTAACAAATTACAAAGAGATACCGATTTTGATTATGTGGTGATTGGGGACCGGCACTCGATCCGCCTTTACCATCCTAATCCGGAGAAAATTGGTTATCCTATGCAGTTCACCAAACAGGGCGCGCTGGAGAAAGGGGAGAGCTACTTCA                                                                                                            >  SRR19973081.1732034/1‑150 (MQ=60)
                                        CTAACAAATTACAAAGAGATACCGATTTTGATTATGTGGTGATTGGGGACCGGCACTCGATCCGCCTTTACCATCCTAATCCGGAGAAAATTGGTTATCCTATGCAGTTAACCAAACAGGGCGCGCTGGAGAAAGGGGAGAGCTACTTCA                                                                                                            >  SRR19973081.792143/1‑150 (MQ=60)
                                        CTAACAAATTACAAAGAGATACCGATTTTGATTATGTGGTGATTGGGGACCGGCACTCGATCCGCCTTTACCATCCTAATCCGGAGAAAATTGGTTATCCTATGCAGTTAACCAAACAGGGCGCGCTGGAGAAAGGGGAGAGCTACTTCA                                                                                                            >  SRR19973081.1355696/1‑150 (MQ=60)
                                        CTAACAAATTACAAAGAGATACCGATTTTGATTATGTGGTGATTGGGGACCGGCACTCGATCCGCCTTTACCATCCTAATCCGGAGAAAATTGGTTATCCTATGCAGTTAACCAAACAGGGCGCGCTGGAGAAAGGGGAGAGCTACTTCA                                                                                                            >  SRR19973081.1554772/1‑150 (MQ=60)
                                         TAACAAATTACAAAGAGATACCGATTTTGATTATGTGGTGATTGGGGACCGGCACTCGATCCGCCTTTACCATCCTAATCCGGAGAAAATTGGTTATCCTATGCAGTTCACCAAACAGGGCGCGCTGGAGAAAGGGGAGAGCTACTTCAT                                                                                                           <  SRR19973081.4416024/150‑1 (MQ=60)
                                         TAACAAATTACAAAGAGATACCGATTTTGATTATGTGGTGATTGGGGACCGGCACTCGATCCGCCTTTACCATCCTAATCCGGAGAAAATTGGTTATCCTATGCAGTTCACCAAACAGGGCGCGCTGGAGAAAGGGGAGAGCTACTTCAT                                                                                                           <  SRR19973081.4416247/150‑1 (MQ=60)
                                          AACAAATTACAAAGAGATACCGATTTTGATTATGTGGTGATTGGGGACCGGCACTCGATCCGCCTTTACCATCCTAATCCGGAGAAAATTGGTTATCCTATGCAGTTCACCAAACAGGGCGCGCTGGAGAAAGGGGAGAGCTACTTCATT                                                                                                          <  SRR19973081.3181207/150‑1 (MQ=60)
                                          AACAAATTACAAAGAGATACCGATTTTGATTATGTGGTGATTGGGGACCGGCACTCGATCCGCCTTTACCATCCTAATCCGGAGAAAATTGGTTATCCTATGCAGTTAACCAAACAGGGCGCGCTGGAGAAAGGGGAGAGCTACTTCATT                                                                                                          <  SRR19973081.1977819/150‑1 (MQ=60)
                                          AACAAATTACAAAGAGATACCGATTTTGATTATGTGGTGATTGGGGACCGGCACTCGATCCGCCTTTACCATCCTAATCCGGAGAAAATTGGTTATCCTATGCAGTTAACCAAACAGGGCGCGCTGGAGAAAGGGGAGAGCTACTTCATT                                                                                                          >  SRR19973081.1897523/1‑150 (MQ=60)
                                          AACAAATTACAAAGAGATACCGATTTTGATTATGTGGTGATTGGGGACCGGCACTCGATCCGCCTTTACCATCCTAATCCGGAGAAAATTGGTTATCCTATGCAGTTAACCAAACAGGGCGCGCTGGAGAAAGGGGAGAGCTACTTCATT                                                                                                          <  SRR19973081.3080519/150‑1 (MQ=60)
                                             AAATTACAAAGAGATACCGATTTTGATTATGTGGTGATTGGGGACCGGCACTCGATCCGCCTTTACCATCCTAATCCGGAGAAAATTGGTTATCCTATGCAGTTAACCAAACAGGGCGCGCTGGAGAAAGGGGAGAGCTACTTCATTACC                                                                                                       >  SRR19973081.2410595/1‑150 (MQ=60)
                                              AATTACAAAGAGATACCGATTTTGATTATGTGGTGATTGGGGACCGGCACTCGATCCGCCTTTACCATCCTAATCCGGAGAAAATTGGTTATCCTATGCAGTTCACCAAACAGGGCGCGCTGGAGAAAGGGGAGAGCTACTTCATTACCG                                                                                                      <  SRR19973081.2512340/150‑1 (MQ=60)
                                              AATTACAAAGAGATACCGATTTTGATTATGTGGTGATTGGGGACCGGCACTCGATCCGCCTTTACCATCCTAATCCGGAGAAAATTGGTTATCCTATGCAGTTCACCAAACAGGGCGCGCTGGAGAAAGGGGAGAGCTACTTCATTACCG                                                                                                      <  SRR19973081.2512232/150‑1 (MQ=60)
                                              AATTACAAAGAGATACCGATTTTGATTATGTGGTGATTGGGGACCGGCACTCGATCCGCCTTTACCATCCTAATCCGGAGAAAATTGGTTATCCTATGCAGTTCACCAAACAGGGCGCGCTGGAGAAAGGGGAGAGCTACTTCATTACCG                                                                                                      <  SRR19973081.70564/150‑1 (MQ=60)
                                               ATTACAAAGAGATACCGATTTTGATTATGTGGTGATTGGGGACCGGCACTCGATCCGCCTTTACCATCCTAATCCGGAGAAAATTGGTTATCCTATGCAGTTCACCAAACAGGGCGCGCTGGAGAAAGGGGAGAGCTACTTCATTACCGG                                                                                                     >  SRR19973081.3938128/1‑150 (MQ=60)
                                               ATTACAAAGAGATACCGATTTTGATTATGTGGTGATTGGGGACCGGCACTCGATCCGCCTTTACCATCCTAATCCGGAGAAAATTGGTTATCCTATGCAGTTCACCAAACAGGGCGCGCTGGAGAAAGGGGAGAGCTACTTCATTACCGG                                                                                                     >  SRR19973081.3938122/1‑150 (MQ=60)
                                                TTACAAAGAGATACCGATTTTGATTATGTGGTGATTGGGGACCGGCACTCGATCCGCCTTTACCATCCTAATCCGGAGAAAATTGGTTATCCTATGCAGTTAACCAAACAGGGCGCGCTGGAGAAAGGGGAGAGCTACTTCATTACCGGG                                                                                                    >  SRR19973081.3611404/1‑150 (MQ=60)
                                                TTACAAAGAGATACCGATTTTGATTATGTGGTGATTGGGGACCGGCACTCGATCCGCCTTTACCATCCTAATCCGGAGAAAATTGGTTATCCTATGCAGTTAACCAAACAGGGCGCGCTGGAGAAAGGGGAGAGCTACTTCATTACCGGG                                                                                                    >  SRR19973081.3611447/1‑150 (MQ=60)
                                                TTACAAAGAGATACCGATTTTGATTATGTGGTGATTGGGGACCGGCACTCGATCCGCCTTTACCATCCTAATCCGGAGAAAATTGGTTATCCTATGCAGTTAACCAAACAGGGCGCGCTGGAGAAAGGGGAGAGCTACTTCATTACCGGG                                                                                                    >  SRR19973081.4369010/1‑150 (MQ=60)
                                                  ACAAAGAGATACCGATTTTGATTATGTGGTGATTGGGGACCGGCACTCGATCCGCCTTTACCATCCTAATCCGGAGAAAATTGGTTATCCTATGCAGTTAACCAAACAGGGCGCGCTGGAGAAAGGGGAGAGCTACTTCATTACCGGGAA                                                                                                  <  SRR19973081.1959235/150‑1 (MQ=60)
                                                   CAAAGAGATACCGATTTTGATTATGTGGTGATTGGGGACCGGCACTCGATCCGCCTTTACCATCCTAATCCGGAGAAAATTGGTTATCCTATGCAGTTAACCAAACAGGGCGCGCTGGAGAAAGGGGAGAGCTACTTCATTACCGGGAAA                                                                                                 >  SRR19973081.374434/1‑150 (MQ=60)
                                                     AAGAGATACCGATTTTGATTATGTGGTGATTGGGGACCGGCACTCGATCCGCCTTTACCATCCTAATCCGGAGAAAATTGGTTATCCTATGCAGTTAACCAAACAGGGCGCGCTGGAGAAAGGGGAGAGCTACTTCATTACCGGGAAAGG                                                                                               <  SRR19973081.78016/150‑1 (MQ=60)
                                                     AAGAGATACCGATTTTGATTATGTGGTGATTGGGGACCGGCACTCGATCCGCCTTTACCATCCTAATCCGGAGAAAATTGGTTATCCTATGCAGTTAACCAAACAGGGCGCGCTGGAGAAAGGGGAGAGCTACTTCATTACCGGGAAAGG                                                                                               >  SRR19973081.631180/1‑150 (MQ=60)
                                                     AAGAGATACCGATTTTGATTATGTGGTGATTGGGGACCGGCACTCGATCCGCCTTTACCATCCTAATCCGGAGAAAATTGGTTATCCTATGCAGTTAACCAAACAGGGCGCGCTGGAGAAAGGGGAGAGCTACTTCATTACCGGGAAAGG                                                                                               <  SRR19973081.2183004/150‑1 (MQ=60)
                                                      AGAGATACCGATTTTGATTATGTGGTGATTGGGGACCGGCACTCGATCCGCCTTTACCATCCTAATCCGGAGAAAATTGGTTATCCTATGCAGTTAACCAAACAGGGCGCGCTGGAGAAAGGGGAGAGCTACTTCATTACCGGGAAAGGG                                                                                              <  SRR19973081.41752/150‑1 (MQ=60)
                                                      AGAGATACCGATTTTGATTATGTGGTGATTGGGGACCGGCACTCGATCCGCCTTTACCATCCTAATCCGGAGAAAATTGGTTATCCTATGCAGTTAACCAAACAGGGCGCGCTGGAGAAAGGGGAGAGCTACTTCATTACCGGGAAAGGG                                                                                              <  SRR19973081.40638/150‑1 (MQ=60)
                                                        AGATACCGATTTTGATTATGTGGTGATTGGGGACCGGCACTCGATCCGCCTTTACCATCCTAATCCGGAGAAAATTGGTTATCCTATGCAGTTCACCAAACAGGGCGCGCTGGAGAAAGGGGAGAGCTACTTCATTACCGGGAAAGGGTC                                                                                            >  SRR19973081.2439333/1‑150 (MQ=60)
                                                           TACCGATTTTGATTATGTGGTGATTGGGGACCGGCACTCGATCCGCCTTTACCATCCTAATCCGGAGAAAATTGGTTATCCTATGCAGTTCACCAAACAGGGCGCGCTGGAGAAAGGGGAGAGCTACTTCATTACCGGGAAAGGGTCAAT                                                                                         <  SRR19973081.4759670/150‑1 (MQ=60)
                                                            ACCGATTTTGATTATGTGGTGATTGGGGACCGGCACTCGATCCGCCTTTACCATCCTAATCCGGAGAAAATTGGTTATCCTATGCAGTTAACCAAACAGGGCGCGCTGGAGAAAGGGGAGAGCTACTTCATTACCGGGAAAGGGTCAATG                                                                                        >  SRR19973081.4704382/1‑150 (MQ=60)
                                                             CCGATTTTGATTATGTGGTGATTGGGGACCGGCACTCGATCCGCCTTTACCATCCTAATCCGGAGAAAATTGGTTATCCTATGCAGTTAACCAAACAGGGCGCGCTGGAGAAAGGGGAGAGCTACTTCATTACCGGGAAAGGGTCAATGG                                                                                       <  SRR19973081.747975/150‑1 (MQ=60)
                                                               GATTTTGATTATGTGGTGATTGGGGACCGGCACTCGATCCGCCTTTACCATCCTAATCCGGAGAAAATTGGTTATCCTATGCAGTTAACCAAACAGGGCGCGCTGGAGAAAGGGGAGAGCTACTTCATTACCGGGAAAGGGTCAATGGGG                                                                                     <  SRR19973081.3508364/150‑1 (MQ=60)
                                                                ATTTTGATTATGTGGTGATTGGGGACCGGCACTCGATCCGCCTTTACCATCCTAATCCGGAGAAAATTGGTTATCCTATGCAGTTAACCAAACAGGGCGCGCTGGAGAAAGGGGAGAGCTACTTCATTACCGGGAAAGGGTCAATGGGGA                                                                                    >  SRR19973081.2827191/1‑150 (MQ=60)
                                                                 TTTTGATTATGTGGTGATTGGGGACCGGCACTCGATCCGCCTTTACCATCCTAATCCGGAGAAAATTGGTTATCCTATGCAGTTCACCAAACAGGGCGCGCTGGAGAAAGGGGAGAGCTACTTCATTACCGGGAAAGGGTCAATGGGGAT                                                                                   >  SRR19973081.2160579/1‑150 (MQ=60)
                                                                   TTGATTATGTGGTGATTGGGGACCGGCACTCGATCCGCCTTTACCATCCTAATCCGGAGAAAATTGGTTATCCTATGCAGTTCACCAAACAGGGCGCGCTGGAGAAAGGGGAGAGCTACTTCATTACCGGGAAAGGGTCAATGGGGATGG                                                                                 >  SRR19973081.2944211/1‑150 (MQ=60)
                                                                   TTGATTATGTGGTGATTGGGGACCGGCACTCGATCCGCCTTTACCATCCTAATCCGGAGAAAATTGGTTATCCTATGCAGTTAACCAAACAGGGCGCGCTGGAGAAAGGGGAGAGCTACTTCATTACCGGGAAAGGGTCAATGGGGATGG                                                                                 >  SRR19973081.603411/1‑150 (MQ=60)
                                                                     GATTATGTGGTGATTGGGGACCGGCACTCGATCCGCCTTTACCATCCTAATCCGGAGAAAATTGGTTATCCTATGCAGTTAACCAAACAGGGCGCGCTGGAGAAAGGGGAGAGCTACTTCATTACCGGGAAAGGGTCAATGGGGATGGCG                                                                               >  SRR19973081.1808177/1‑150 (MQ=60)
                                                                     GATTATGTGGTGATTGGGGACCGGCACTCGATCCGCCTTTACCATCCTAATCCGGAGAAAATTGGTTATCCTATGCAGTTAACCAAACAGGGCGCGCTGGAGAAAGGGGAGAGCTACTTCATTACCGGGAAAGGGTCAATGGGGATGGCG                                                                               >  SRR19973081.165100/1‑150 (MQ=60)
                                                                         ATGTGGTGATTGGGGACCGGCACTCGATCCGCCTTTACCATCCTAATCCGGAGAAAATTGGTTATCCTATGCAGTTCACCAAACAGGGCGCGCTGGAGAAAGGGGAGAGCTACTTCATTACCGGGAAAGGGTCAATGGGGATGGCGATGC                                                                           <  SRR19973081.2628323/150‑1 (MQ=60)
                                                                          TGTGGTGATTGGGGACCGGCACTCGATCCGCCTTTACCATCCTAATCCGGAGAAAATTGGTTATCCTATGCAGTTAACCAAACAGGGCGCGCTGGAGAAAGGGGAGAGCTACTTCATTACCGGGAAAGGGTCAATGGGGATGGCGATGCG                                                                          >  SRR19973081.1582387/1‑150 (MQ=60)
                                                                                GATTGGGGACCGGCACTCGATCCGCCTTTACCATCCTAATCCGGAGAAAATTGGTTATCCTATGCAGTTAACCAAACAGGGCGCGCTGGAGAAAGGGGAGAGCTACTTCATTACCGGGAAAGGGTCAATGGGGATGGCGATGCGCGCCAA                                                                    <  SRR19973081.961781/150‑1 (MQ=60)
                                                                                GATTGGGGACCGGCACTCGATCCGCCTTTACCATCCTAATCCGGAGAAAATTGGTTATCCTATGCAGTTAACCAAACAGGGCGCGCTGGAGAAAGGGGAGAGCTACTTCATTACCGGGAAAGGGTCAATGGGGATGGCGATGCGCGCCAA                                                                    <  SRR19973081.962025/150‑1 (MQ=60)
                                                                                 ATTGGGGACCGGCACTCGATCCGCCTTTACCATCCTAATCCGGAGAAAATTGGTTATCCTATGCAGTTAACCAAACAGGGCGCGCTGGAGAAAGGGGAGAGCTACTTCATTACCGGGAAAGGGTCAATGGGGATGGCGATGCGCGCCAAA                                                                   >  SRR19973081.142272/1‑150 (MQ=60)
                                                                                   TGGGGACCGGCACTCGATCCGCCTTTACCATCCTAATCCGGAGAAAATTGGTTATCCTATGCAGTTAACCAAACAGGGCGCGCTGGAGAAAGGGGAGAGCTACTTCATTACCGGGAAAGGGTCAATGGGGATGGCGATGCGCGCCAAAAC                                                                 >  SRR19973081.3243716/1‑150 (MQ=60)
                                                                                       GACCGGCACTCGATCCGCCTTTACCATCCTAATCCGGAGAAAATTGGTTATCCTTTGCAGTTAACCAAACAGGGCGCGCTGGAGAAAGGGGAGAGCTACTTCATTACCGGGAAAGGGTCAATGGGGATGGCGATGCGCGCCAAAACGCCA                                                             <  SRR19973081.606597/150‑1 (MQ=60)
                                                                                       GACCGGCACTCGATCCGCCTTTACCATCCTAATCCGGAGAAAATTGGTTATCCTATGCAGTTCACCAAACAGGGCGCGCTGGAGAAAGGGGAGAGCTACTTCATTACCGGGAAAGGGTCAATGGGGATGGCGATGCGCGCCAAAACGCCA                                                             <  SRR19973081.3418377/150‑1 (MQ=60)
                                                                                       GACCGGCACTCGATCCGCCTTTACCATCCTAATCCGGAGAAAATTGGTTATCCTATGCAGTTAACCAAACAGGGCGCGCTGGAGAAAGGGGAGAGCTACTTCATTACCGGGAAAGGGTCAATGGGGATGGCGATGCGCGCCAAAACGCCA                                                             <  SRR19973081.607897/150‑1 (MQ=60)
                                                                                        ACCGGCACTCGATCCGCCTTTACCATCCTAATCCGGAGAAAATTGGTTATCCTATGCAGTTCACCAAACAGGGCGCGCTGGAGAAAGGGGAGAGCTACTTCATTACCGGGAAAGGGTCAATGGGGATGGCGATGCGCGCCAAAACGCCAA                                                            >  SRR19973081.800180/1‑150 (MQ=60)
                                                                                        ACCGGCACTCGATCCGCCTTTACCATCCTAATCCGGAGAAAATTGGTTATCCTATGCAGTTAACCAAACAGGGCGCGCTGGAGAAAGGGGAGAGCTACTTCATTACCGGGAAAGGGTCAATGGGGATGGCGATGCGCGCCAAAACGCCAA                                                            >  SRR19973081.1938428/1‑150 (MQ=60)
                                                                                        ACCGGCACTCGATCCGCCTTTACCATCCTAATCCGGAGAAAATTGGTTATCCTATGCAGTTAACCAAACAGGGCGCGCTGGAGAAAGGGGAGAGCTACTTCATTACCGGGAAAGGGTCAATGGGGATGGCGATGCGCGCCAAAACGCCAA                                                            >  SRR19973081.1938442/1‑150 (MQ=60)
                                                                                          CGGCACTCGATCCGCCTTTACCATCCTAATCCGGAGAAAATTGGTTATCCTATGCAGTTAACCAAACAGGGCGCGCTGGAGAAAGGGGAGAGCTACTTCATTACCGGGAAAGGGTCAATGGGGATGGCGATGCGCGCCAAAACGCCAATC                                                          >  SRR19973081.4564231/1‑150 (MQ=60)
                                                                                            GCACTCGATCCGCCTTTACCATCCTAATCCGGAGAAAATTGGTTATCCTATGCAGTTAACCAAACAGGGCGCGCTGGAGAAAGGGGAGAGCTACTTCATTACCGGGAAAGGGTCAATGGGGATGGCGATGCGCGCCAAAACGCCAATCTT                                                        <  SRR19973081.3927475/150‑1 (MQ=60)
                                                                                             CACTCGATCCGCCTTTACCATCCTAATCCGGAGAAAATTGGTTATCCTATGCAGTTAACCAAACAGGGCGCGCTGGAGAAAGGGGAGAGCTACTTCATTACCGGGAAAGGGTCAATGGGGATGGCGATGCGCGCCAAAACGCCAATCTTT                                                       >  SRR19973081.241062/1‑150 (MQ=60)
                                                                                             CACTCGATCCGCCTTTACCATCCTAATCCGGAGAAAATTGGTTATCCTATGCAGTTAACCAAACAGGGCGCGCTGGAGAAAGGGGAGAGCTACTTCATTACCGGGAAAGGGTCAATGGGGATGGCGATGCGCGCCAAAACGCCAATCTTT                                                       >  SRR19973081.2686578/1‑150 (MQ=60)
                                                                                              ACTCGATCCGCCTTTACCATCCTAATCCGGAGAAAATTGGTTATCCTATGCAGTTCACCAAACAGGGCGCGCTGGAGAAAGGGGAGAGCTACTTCATTACCGGGAAAGGGTCAATGGGGATGGCGATGCGCGCCAAAACGCCAATCTTTG                                                      >  SRR19973081.1878849/1‑150 (MQ=60)
                                                                                                TCGATCCGCCTTTACCATCCTAATCCGGAGAAAATTGGTTATCCTATGCAGTTAACCAAACAGGGCGCGCTGGAGAAAGGGGAGAGCTACTTCATTACCGGGAAAGGGTCAATGGGGATGGCGATGCGCGCCAAAACGCCAATCTTTGAT                                                    <  SRR19973081.3169236/150‑1 (MQ=60)
                                                                                                 CGATCCGCCTTTACCATCCTAATCCGGAGAAAATTGGTTATCCTATGCAGTTAACCAAACAGGGCGCGCTGGAGAAAGGGGAGAGCTACTTCATTACCGGGAAAGGGTCAATGGGGATGGCGATGCGCGCCAAAACGCCAATCTTTGATG                                                   >  SRR19973081.3702905/1‑150 (MQ=60)
                                                                                                  GATCCGCCTTTACCATCCTAATCCGGAGAAAATTGGTTATCCTATGCAGTTCACCAAACAGGGCGCGCTGGAGAAAGGGGAGAGCTACTTCATTACCGGGAAAGGGTCAATGGGGATGGCGATGCGCGCCAAAACGCCAATCTTTGATGA                                                  >  SRR19973081.196266/1‑150 (MQ=60)
                                                                                                  GATCCGCCTTTACCATCCTAATCCGGAGAAAATTGGTTATCCTATGCAGTTCACCAAACAGGGCGCGCTGGAGAAAGGGGAGAGCTACTTCATTACCGGGAAAGGGTCAATGGGGATGGCGATGCGCGCCAAAACGCCAATCTTTGATGA                                                  >  SRR19973081.197329/1‑150 (MQ=60)
                                                                                                    TCCGCCTTTACCATCCTAATCCGGAGAAAATTGGTTATCCTATGCAGTTCACCAAACAGGGCGCGCTGGAGAAAGGGGAGAGCTACTTCATTACCGGGAAAGGGTCAATGGGGATGGCGATGCGCGCCAAAACGCCAATCTTTGATGACG                                                <  SRR19973081.4506940/150‑1 (MQ=60)
                                                                                                              CCATCCTAATCCGGAGAAAATTGGTTATCCTATGCAGTTAACCAAACAGGGCGCGCTGGAGAAAGGGGAGAGCTACTTCATTACCGGGAAAGGGTCAATGGGGATGGCGATGCGCGCCAAAACGCCAATCTTTGATGACGATGGAAAAGT                                      >  SRR19973081.2080844/1‑150 (MQ=60)
                                                                                                                  CCTAATCCGGAGAAAATTGGTTATCCTATGCAGTTCACCAAACAGGGCGCGCTGGAGAAAGGGGAGAGCTACTTCATTACCGGGAAAGGGTCAATGGGGATGGCGATGCGCGCCAAAACGCCAATCTTTGATGACGATGGAAAAGTCATC                                  >  SRR19973081.4545445/1‑150 (MQ=60)
                                                                                                                  CCTAATCCGGAGAAAATTGGTTATCCTATGCAGTTAACCAAACAGGGCGCGCTGGAGAAAGGGGAGAGCTACTTCATTACCGGGAAAGGGTCAATGGGGATGGCGATGCGCGCCAAAACGCCAATCTTTGATGACGATGGAAAAGTCATC                                  >  SRR19973081.1409566/1‑150 (MQ=60)
                                                                                                                     AATCCGGAGAAAATTGGTTATCCTATGCAGTTAACCAAACAGGGCGCGCTGGAGAAAGGGGAGAGCTACTTCATTACCGGGAAAGGGTCAATGGGGATGGCGATGCGCGCCAAAACGCCAATCTTTGATGACGATGGAAAAGTCATCGGC                               <  SRR19973081.3021764/150‑1 (MQ=60)
                                                                                                                          GGAGAAAATTGGTTATCCTATGCAGTTAACCAAACAGGGCGCGCTGGAGAAAGGGGAGAGCTACTTCATTACCGGGAAAGGGTCAATGGGGATGGCGATGCGCGCCAAAACGCCAATCTTTGATGACGATGGAAAAGTCATCGGCGTGGT                          <  SRR19973081.3484657/150‑1 (MQ=60)
                                                                                                                             GAAAATTGGTTATCCTATGCAGTTAACCAAACAGGGCGCGCTGGAGAAAGGGGAGAGCTACTTCATTACCGGGAAAGGGTCAATGGGGATGGCGATGCGCGCCAAAACGCCAATCTTTGATGACGATGGAAAAGTCATCGGCGTGGTGTC                       <  SRR19973081.739312/150‑1 (MQ=60)
                                                                                                                                 ATTGGTTATCCTATGCAGTTAACCAAACAGGGCGCGCTGGAGAAAGGGGAGAGCTACTTCATTACCGGGAAAGGGTCAATGGGGATGGCGATGCGCGCCAAAACGCCAATCTTTGATGACGATGGAAAAGTCATCGGCGTGGTGTCGATT                   <  SRR19973081.3789718/150‑1 (MQ=60)
                                                                                                                                  TTGGTTATCCTATGCAGTTCACCAAACAGGGCGCGCTGGAGAAAGGGGAGAGCTACTTCATTACCGGGAAAGGGTCAATGGGGATGGCGATGCGCGCCAAAACGCCAATCTTTGATGACGATGGAAAAGTCATCGGCGTGGTGTCGATTG                  <  SRR19973081.1814622/150‑1 (MQ=60)
                                                                                                                                   TGGTTATCCTATGCAGTTAACCAAACAGGGCGCGCTGGAGAAAGGGGAGAGCTACTTCATTACCGGGAAAGGGTCAATGGGGATGGCGATGCGCGCCAAAACGCCAATCTTTGATGACGATGGAAAAGTCATCGGCGTGGTGTCGATTGG                 <  SRR19973081.2058696/150‑1 (MQ=60)
                                                                                                                                   TGGTTATCCTATGCAGTTAACCAAACAGGGCGCGCTGGAGAAAGGGGAGAGCTACTTCATTACCGGGAAAGGGTCAATGGGGATGGCGATGCGCGCCAAAACGCCAATCTTTGATGACGATGGAAAAGTCATCGGCGTGGTGTCGATTGG                 <  SRR19973081.2058691/150‑1 (MQ=60)
                                                                                                                                     TTTATCCTATGCAGTTAACCAAACAGGGCGCGCTGGAGAAAGGGGAGAGCTACTTCATTACCGGGAAAGGGTCAATGGGGATGGCGATGCGCGCCAAAACGACAATCTTTGATGACGATGGAAAAGTCATCGGCGTGGTGTCGATTGGCT               <  SRR19973081.4894214/150‑1 (MQ=60)
                                                                                                                                     GTTATCCTATGCAGTTAACCAAACAGGGCGCGCTGGAGAAAGGGGAGAGCTACTTCATTACCGGGAAAGGGTCAATGGGGATGGCGATGCGCGCCAAAACGCCAATCTTTGATGACGATGGAAAAGTCATCGGCGTGGTGTCGATTGGCT               <  SRR19973081.3538966/150‑1 (MQ=60)
                                                                                                                                     GTTATCCTATGCAGTTAACCAAACAGGGCGCGCTGGAGAAAGGGGAGAGCTACTTCATTACCGGGAAAGGGTCAATGGGGATGGCGATGCGCGCCAAAACGCCAATCTTTGATGACGATGGAAAAGTCATCGGCGTGGTGTCGATTGGCT               <  SRR19973081.1917031/150‑1 (MQ=60)
                                                                                                                                      TTATCCTATGCAGTTCACCAAACAGGGCGCGCTGGAGAAAGGGGAGAGCTACTTCATTACCGGGAAAGGGTCAATGGGGATGGCGATGCGCGCCAAAACGCCAATCTTTGATGACGATGGAAAAGTCATCGGCGTGGTGTCGATTGGCTA              >  SRR19973081.4015865/1‑150 (MQ=60)
                                                                                                                                      TTATCCTATGCAGTTAACCAAACAGGGCGCGCTGGAGAAAGGGGAGAGCTACTTCATTACCGGGAAAGGGTCAATGGGGATGGCGATGCGCGCCAAAACGCCAATCTTTGATGACGATGGAAAAGTCATCGGCGTGGTGTCGATTGGCTA              <  SRR19973081.4368283/150‑1 (MQ=60)
                                                                                                                                       TATCCTATGCAGTTCACCAAACAGGGCGCGCTGGAGAAAGGGGAGAGCTACTTCATTACCGGGAAAGGGTCAATGGGGATGGCGATGCGCGCCAAAACGCCAATCTTTGATGACGATGGAAAAGTCATCGGCGTGGTGTCGATTGGCTAC             >  SRR19973081.653657/1‑150 (MQ=60)
                                                                                                                                        ATCCTATGCAGTTCACCAAACAGGGCGCGCTGGAGAAAGGGGAGAGCTACTTCATTACCGGGAAAGGGTCAATGGGGATGGCGATGCGCGCCAAAACGCCAATCTTTGATGACGATGGAAAAGTCATCGGCGTGGTGTCGATTGGCTACC            <  SRR19973081.2662276/150‑1 (MQ=60)
                                                                                                                                        ATCCTATGCAGTTAACCAAACAGGGCGCGCTGGAGAAAGGGGAGAGCTACTTCATTACCGGGAAAGGGTCAATGGGGATGGCGATGCGCGCCAAAACGCCAATCTTTGATGACGATGGACAAGTCATCGGCGTGGTGTCGATTGGCTACC            >  SRR19973081.4618510/1‑150 (MQ=60)
                                                                                                                                        ATCCTATGCAGTTAACCAAACAGGGCGCGCTGGAGAAAGGGGAGAGCTACTTCATTACCGGGAAAGGGTCAATGGGGATGGCGATGCGCGCCAAAACGCCAATCTTTGATGACGATGGAAAAGTCATCGGCGTGGTGTCGATTGGCTACC            >  SRR19973081.228656/1‑150 (MQ=60)
                                                                                                                                             ATGCAGTTAACCAAACCGGGCGCGCTGGAGAAAGGGGAGAGCTACTTCATTACCGGGAAAGGGTCAATGGGGATGGCGATGCGCGCCAAAACGCCAATCTTTGATGACGATGGAAAAGTCATCGGCGTGGTGTCGATTGGCTACCTGGTG       <  SRR19973081.3310920/150‑1 (MQ=60)
                                                                                                                                             ATGCAGTTAACCAAACAGGGCGCGCTGGAGAAAGGGGAGAGCTACTTCATTACCGGGAAAGGGTCAATGGGGATGGCGATGCGCGCCAAAACGCCAATCTTTGATGACGATGGAAAAGTCATCGGCGTGGTGTCGATTGGCTACCTGGTG       <  SRR19973081.3313280/150‑1 (MQ=60)
                                                                                                                                              TGCAGTTCACCAAACAGGGCGCGCGGGAGAAAGGGGAGAGCTACTTCATTACCGGGAAAGGGTCAATGGGGATGGCGATGCGCGCCAAAACGCCAATCTTTGATGACGATGGAAAAGTCATCGGCGTGGTGTCGATTGGCTACCTGGTGA      <  SRR19973081.1304148/150‑1 (MQ=60)
                                                                                                                                                CAGTTAACCAAACAGGGCGCGCTGGAGAAAGGGGAGAGCTACTTCATTACCGGGAAAGGGTCAATGGGGATGGTGATGCGCGCCAAAACGCCAATCTTTGATGACGATGGAAAAGTCATCGGCGTGGTGTCGATTGGCTACCTGGTGAGT    >  SRR19973081.113863/1‑150 (MQ=60)
                                                                                                                                                 AGTTCACCAAACAGGGCGCGCTGGAGAAAGGGGAGAGCTACTTCATTACCGGGAAAGGGTCAATGGGGATGGCGATGCGCGCCAAAACGCCAATCTTTGATGACGATGGAAAAGTCATCGGCGTGGTGTCGATTGGCTACCTGGTGAGTA   >  SRR19973081.384769/1‑150 (MQ=60)
                                                                                                                                                 AGTTCACCAAACAGGGCGCGCTGGAGAAAGGGGAGAGCTACTTCATTACCGGGAAAGGGTCAATGGGGATGGCGATGCGCGCCAAAACGCCAATCTTTGATGACGATGGAAAAGTCATCGGCGTGGTGTCGATTGGCTACCTGGTGAGTA   >  SRR19973081.384829/1‑150 (MQ=60)
                                                                                                                                                 AGTTCACCAAACAGGGCGCGCTGGAGAAAGGGGAGAGCTACTTCATTACCGGGAAAGGGTCAATGGGGATGGCGATGCGCGCCAAAACGCCAATCTTTGATGACGATGGAAAAGTCATCGGCGTGGTGTCGATTGGCTACCTGGTGAGTA   >  SRR19973081.2829187/1‑150 (MQ=60)
                                                                                                                                                  GTTAACCAAACAGGGCGCGCTGGAGAAAGGGGAGAGCTACTTCATTACCGGGAAAGGGTCAATGGGGATGGCGATGCGCGCCAAAACGCCAATCTTTGATGACGATGGAAAAGTCATCGGCGTGGTGTCGATTGGCTACCTGGTGAGTAA  <  SRR19973081.1038397/150‑1 (MQ=60)
                                                                                                                                                  GTTAACCAAACAGGGCGCGCTGGAGAAAGGGGAGAGCTACTTCATTACCGGGAAAGGGTCAATGGGGATGGCGATGCGCGCCAAAACGCCAATCTTTGATGACGATGGAAAAGTCATCGGCGTGGTGTCGATTGGCTACCTGGTGAGTAA  >  SRR19973081.298561/1‑150 (MQ=60)
                                                                                                                                                  GTTAACCAAACAGGGCGCGCTGGAGAAAGGGGAGAGCTACTTCATTACCGGGAAAGGGTCAATGGGGATGGCGATGCGCGCCAAAACGCCAATCTTTGATGACGATGGAAAAGTCATCGGCGTGGTGTCGATTGGCTACCTGGTGAGTAA  >  SRR19973081.298535/1‑150 (MQ=60)
                                                                                                                                                  GTTAACCAAACAGGGCGCGCTGGAGAAAGGGGAGAGCTACTTCATTACCGGGAAAGGGTCAATGGGGATGGCGATGCGCGCCAAAACGCCAATCTTTGATGACGATGGAAAAGTCATCGGCGTGGTGTCGATTGGCTACCTGGTGAGTAA  <  SRR19973081.1039811/150‑1 (MQ=60)
                                                                                                                                                     |                                                                                                                                                  
GCGGTGAAAACGCGTGACTACAAACGGCTGGCGACCATCGCTAACAAATTACAAAGAGATACCGATTTTGATTATGTGGTGATTGGGGACCGGCACTCGATCCGCCTTTACCATCCTAATCCGGAGAAAATTGGTTATCCTATGCAGTTCACCAAACAGGGCGCGCTGGAGAAAGGGGAGAGCTACTTCATTACCGGGAAAGGGTCAATGGGGATGGCGATGCGCGCCAAAACGCCAATCTTTGATGACGATGGAAAAGTCATCGGCGTGGTGTCGATTGGCTACCTGGTGAGTAA  >  CP009273/647907‑648202

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑