Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F98 I0 R1	799	294.1	9752082	92.2%	8991419	150.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	955,662	G→A	30.9%	intergenic (+111/‑58)	aroA → / → ycaL	5‑enolpyruvylshikimate‑3‑phosphate synthetase/putative peptidase‑related chaperone

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	955,662	0	G	A	30.9%	167.3 / 101.1	121	intergenic (+111/‑58)	aroA/ycaL	5‑enolpyruvylshikimate‑3‑phosphate synthetase/putative peptidase‑related chaperone
Reads supporting (aligned to +/- strand): ref base G (19/60); new base A (8/29); total (32/89)
Fisher's exact test for biased strand distribution p-value = 8.19e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 123 total aligned reads displayed.

TATTTCGAGCAGCTGGCGCGGATTAGCCAGGCAGCCTGAATGAACAACGGGCAATAAATAGCCAAATCTTTCTTTATCAAAACGTCGGCACATTGTCGGCGTTTTTTTTCGGACCTTGTGAGTCATTTTGATTAATGGTAGCGTCGCTTGTCAATGTAAGTTGTTGATACATAATATTTATATATGATTAATCAACGGATGATTCACATGAAGAATACTAAATTACTGCTGGCGATTGCGACCTCTGCAGCATTACTGACAGGGTGTCAAAATACCCACGGTATTGATACCAATAT  >  CP009273/955513‑955808

  tttCGAGCAGCTGGCGCGGATTAGCCAGGCAGCCTGAATGAACAACGGGCAATAAATAGCCAAATCTTTCTTTATCAAAACGTCGGCACATTGTCGGCGTTTTTTTTAGGAACATGTGTGTGATTTTGATTTATTGTATCGTGGCgtgtg                                                                                                                                                  >  1:4687726/1‑149 (MQ=255)

   ttCGAGCAGCTGGCGCGGATTAGCCAGGCAGCCTGAATGAACAACGGGCAATAAATAGCCAAATCTTTCTTTATCAAAACGTCGGCACATTGTCGGCGTTTTTTTTCGGACCTTGTGAGTCATTTTGATTAATGGTAGCGTCGCTTGTCa                                                                                                                                                 <  2:4218199/150‑1 (MQ=255)

    tCGAGCAGCTGGCGCGGATTAGCCAGGCAGCCTGAATGAACAACGGGCAATAAATAGCCAAATCTTTCTTTATCAAAACGTCGGCACATTGTCGGCGTTTTTTTTCGGAACTTGTGAGTCATTTTTTTTAATGGTAGCGTCGCGTATCaa                                                                                                                                                >  1:3220743/1‑150 (MQ=255)

    tCGAGCAGCTGGCGCGGATTAGCCAGGCAGCCTGAATGAACAACGGGCAATAAATAGCCAAATCTTTCTTTATCAAAACGTCGGCACATTGTCGGCG‑TTTTTTTCGGACCTTGTGAGTCAATTTGTTTAATTGTAGCGTCGCTTGTaaaa                                                                                                                                               >  1:4233149/1‑149 (MQ=255)

    tCGAGCAGCTGGCGCGGATTAGCCAGGCAGCCTGAATGAACAACGGGCAATAAATAGCCAAATCTTTCTTTATCAAAACGTCGGCACATTGTCGGCG‑TTTTTTTAGGACCATGTGAGTCATTTTGATTAATGGTAGCGTCGCGTGTCaaa                                                                                                                                               >  1:4233169/1‑149 (MQ=255)

        gcagcTGGCGCGGATTAGCCAGGCAGCCTGAATGAACAACGGGCAATAAATAGCCAAATCTTTCTTTATCAAAACGTCGGCACATTGTCGGCGTTTTTTTTCGGACCTTGTGAGTCATTTTGATTAATGGTAGCGTCGCTTGTCAATGTa                                                                                                                                            <  2:4796871/150‑1 (MQ=255)

            cTGGCGCGGATTAGCCAGGCAGCCTGAATGAACAACGGGCAATAAATAGCCAAATCTTTCTTTATCAAAACGTCGGCACATTGTCGGCGTTTTTTTTCGGACCTTGTGAGTCATTTTGATTAATGGTAGCGTCGCTTATCAATGTAAgtt                                                                                                                                        <  2:560821/150‑1 (MQ=255)

            cTGGCGCGGATTAGCCAGGCAGCCTGAATGAACAACGGGCAATAAATAGCCAAATCTTTCTTTATCAAAACGTCGGCACATTGTCGGCGTTTTTTTTCGGACCTTGTGAGTCATTTTGATTAATGGTAGCGTCGCTTATCAATGTAAgtt                                                                                                                                        <  2:560757/150‑1 (MQ=255)

             tGGCGCGGATTAGCCAGGCAGCCTGAATGAACAACGGGCAATAAATAGCCAAATCTTTCTTTATCAAAACGTCGGCACATTGTCGGCGTTTTTTTTCGGACCTTGTGAGTCATTTTGATTAATGGTAGCGTCGCTTGTCAATGTAAgttg                                                                                                                                       <  1:4128439/150‑1 (MQ=255)

             tGGCGCGGATTAGCCAGGCAGCCTGAATGAACAACGGGCAATAAATAGCCAAATCTTTCTTTATCAAAACGTCGGCACATTGTCGGCGTTTTTTTTCGGACCTTGTGAGTCATTTTGATTAATGGTAGCGTCGCTTGTCAATGTAAgttg                                                                                                                                       <  1:4675103/150‑1 (MQ=255)

             tGGCGCGGATTAGCCAGGCAGCCTGAATGAACAACGGGCAATAAATAGCCAAATCTTTCTTTATCAAAACGTCGGCACATTGTCGGCGTTTTTTTTCGGACCTTGTGAGTCATTTTGATTAATGGTAGCGTCGCTTATCAATGTAAgttg                                                                                                                                       <  1:116811/150‑1 (MQ=255)

             tGGCGCGGATTAGCCAGGCAGCCTGAATGAACAACGGGCAATAAATAGCCAAATCTTTCTTTATCAAAACGTCGGCACATTGTCGGCGTTTTTTTTCGGACCTTGTGAGTCATTTTGATTAATGGTAGCGTCGCTTATCAATGTAAgttg                                                                                                                                       <  1:2578281/150‑1 (MQ=255)

             tGGCGCGGATTAGCCAGGCAGCCTGAATGAACAACGGGCAATAAATAGCCAAATCTTTCTTTATCAAAACGTCGGCACATTGTCGGCGTTTTTTTTAGGACCTTTTTATTAATTTTGATTAAATGTATCGTCGCGTTTGAAAGTAAattt                                                                                                                                       >  1:3937306/1‑149 (MQ=255)

             tGGCGCGGATTAGCCAGGCAGCCTGAATGAACAACGGGCAATAAATAGCCAAATCTTTCTTTATCAAAACGTCGGCACATTGTCGGCGTTTTTTTTAGGACCTTTTGTGTCATTTTGTTTAATTGTATCGTCGCGTTTGAATTTTAgttt                                                                                                                                       >  1:3936221/1‑149 (MQ=255)

                cgcgGATTAGCCAGGCAGCCTGAATGAACAACGGGCAATAAATAGCCAAATCTTTCTTTATCAAAACGTCGGCACATTGTCGGCGTTTTTTTTCGGACCTTGTGAGTCATTTTGATTAATGGTAGCGTCGCTTGTCAATGTAAGttgttg                                                                                                                                    <  1:2416461/150‑1 (MQ=255)

                cgcgGATTAGCCAGGCAGCCTGAATGAACAACGGGCAATAAATAGCCAAATCTTTCTTTATCAAAACGTCGGCACATTGTCGGCGTTTTTTTTCGGACCTTGTGAGTCATTTTGATTAATGGTAGCGTCGCTTGTCAATGTAAGttgttg                                                                                                                                    <  1:3540540/150‑1 (MQ=255)

                cgcgGATTAGCCAGGCAGCCTGAATGAACAACGGGCAATAAATAGCCAAATCTTTCTTTATCAAAACGTCGGCACATTGTCGGCGTTTTTTTTCGGACCTTGTGAGTCATTTTGATTAATGGTAGCGTCGCTTGTCAATGTAAGttgttg                                                                                                                                    <  1:1085480/150‑1 (MQ=255)

                cgcgGATTAGCCAGGCAGCCTGAATGAACAACGGGCAATAAATAGCCAAATCTTTCTTTATCAAAACGTCGGCACATTGTCGGCGTTTTTTTTCGGACCTTGTGAGTCATTTTGATTAATGGTAGCGTCGCTTGTCAATGTAAGttgttg                                                                                                                                    <  1:1085478/150‑1 (MQ=255)

                cgcgGATTAGCCAGGCAGCCTGAATGAACAACGGGCAATAAATAGCCAAATCTTTCTTTATCAAAACGTCGGCACATTGTCGGCGTTTTTTTTCGGACCTTGTGAGTCATTTTGATTAATGGTAGCGTCGCTTGTCAATGTAAGttgttg                                                                                                                                    <  1:1908363/150‑1 (MQ=255)

                 gcgGATTAGCCAGGCAGTCTGAATGAACAACGGGCAATAAATAGCCAAATCTTTCTTTATCAAAACGTCGGCACATTGTCGGCGTTTTTTTTGGGACCTTTTGTTTAATTTTTATTAATTGTAGCGGCGCGTATAAATTTAAtttttttt                                                                                                                                   >  1:751824/1‑148 (MQ=255)

                  cgGATTAGCCAGGCAGCCTGAATGAACAACGGGCAATAAATAGCCAAATCTTTCTTTATCAAAACGTCGGCACATTGTCGGCGTTTTTTTTCGGACCTTGTGAGTCATTTTGATTAATGGTAGCGTCGCTTGTCAATGTAAGTTGTTGAt                                                                                                                                  <  1:2364839/150‑1 (MQ=255)

                  cgGATTAGCCAGGCAGCCTGAATGAACAACGGGCAATAAATAGCCAAATCTTTCTTTATCAAAACGTCGGCACATTGTCGGCGTTTTTTTTCGGACCTTGTGAGTCATTTTGATTAATGGTAGCGTCGCTTGTCAATGTAAGTTGTTGAt                                                                                                                                  <  1:2367351/150‑1 (MQ=255)

                    gATTAGCCAGGCAGCCTGAATGAACAACGGGCAATAAATAGCCAAATCTTTCTTTATCAAAACGTCGGCACATTGTCGGCGTTTTTTTTCGGACCTTGTGAGTCATTTTGATTAATGGTAGCGTCGCTTGTCAATGTAAGTTGTTGATAc                                                                                                                                <  2:532464/150‑1 (MQ=255)

                       tAGCCAGGCAGCCTGAATGAACAACGGGCAATAAATAGCCAAATCTTTCTTTATCAAAACGTCGGCACATTGTCGGCGTTTTTTTTCGGACCTTGTGAGTCATTTTGATTAATGGTAGCGTCGCTTGTCAATGTAAGTTGTTGATACata                                                                                                                             <  2:3823012/150‑1 (MQ=255)

                        aGCCAGGCAGCCTGAATGAACAACGGGCAATAAATAGCCAAATCTTTCTTTATCAAAACGTCGGCACATTGTCGGCGTTTTTTTTCGGACCTTGTGAGTCATTTTGATTAATGGTAGCGTCGCTTGTCAATGTAAGTTGTTGATACataa                                                                                                                            <  1:4378540/150‑1 (MQ=255)

                        aGCCAGGCAGCCTGAATGAACAACGGGCAATAAATAGCCAAATCTTTCTTTATCAAAACGTCGGCACATTGTCGGCGTTTTTTTTCGGACCTTGTGAGTCATTTTGATTAATGGTAGCGTCGCTTGTCAATGTAAGTTGTTGATACataa                                                                                                                            <  1:2514795/150‑1 (MQ=255)

                             ggCAGCCTGAATGAACAACGGGCAATAAATAGCCAAATCTTTCTTTATCAAAACGTCGGCACATTGTCGGCGTTTTTTTTCGGACCTTGTGAGTCATTTTGATTAATGGTAGCGTCGCTTGTCAATGTAAGTTGTTGATACATAATAttt                                                                                                                       <  1:3448109/150‑1 (MQ=255)

                             ggCAGCCTGAATGAACAACGGGCAATAAATAGCCAAATCTTTCTTTATCAAAACGTCGGCACATTGTCGGCGTTTTTTTTCGGACCTTGTGAGTCATTTTGATTAATGGTAGCGTCGCTTGTCAATGTAAGTTGTTGATACATAATAttt                                                                                                                       <  1:1546684/150‑1 (MQ=255)

                             ggCAGCCTGAATGAACAACGGGCAATAAATAGCCAAATCTTTCTTTATCAAAACGTCGGCACATTGTCGGCGTTTTTTTTCGGACCTTGTGAGTCATTTTGATTAATGGTAGCGTCGCTTATCAATGTAAGTTGTTGATACATAATAttt                                                                                                                       <  2:3290910/150‑1 (MQ=255)

                                aGCCTGAATGAACAACGGGCAATAAATAGCCAAATCTTTCTTTATCAAAACGTCGGCACATTGTCGGCGTTTTTTTTCGGACCTTGTGAGTCATTTTGATTAATGGTAGCGTCGCTTATCAATGTAAGTTGTTGATACATAATATTtata                                                                                                                    <  1:1301201/150‑1 (MQ=255)

                                    tgaatgaaCAACGGGCAATAAATAGCCAAATCTTTCTTTATCAAAACGTCGGCACATTGTCGGCGTTTTTTTTAGGACCATTTGATTCATTTTTATTAATTGTAGCGTCGCGTTTAAAATTAAGTTTTTGTTAAAAAAAAATtatataag                                                                                                                >  2:2570586/1‑148 (MQ=255)

                                     gaatgaaCAACGGGCAATAAATAGCCAAATCTTTCTTTATCAAAACGTCGGCACATTGTCGGCGTTTTTTTTCGGACCTTGTGAGTCATTTTGATTAATGGTAGCGTCGCTTGTCAATGTAAGTTGTTGATACATAATATTTATATATGa                                                                                                               <  2:116801/150‑1 (MQ=255)

                                      aatgaaCAACGGGCAATAAATAGCCAAATCTTTCTTTATCAAAACGTCGGCACATTGTCGGCGTTTTTTTTCGGACCTTGTGAGTCATTTTGATTAATGGTAGCGTCGCTTGTCAATGTAAGTTGTTGATACATAATATTTATATATGAt                                                                                                              <  2:4107376/150‑1 (MQ=255)

                                      aatgaaCAACGGGCAATAAATAGCCAAATCTTTCTTTATCAAAACGTCGGCACATTGTCGGCGTTTTTTTTCGGACCTTGTGAGTCATTTTGATTAATGGTAGCGTCGCTTGTCAATGTAAGTTGTTGATACATAATATTTATATATGAt                                                                                                              <  1:4141107/150‑1 (MQ=255)

                                       atgaaCAACGGGCAATAAATAGCCAAATCTTTCTTTATCAAAACGTCGGCACATTGTCGGCGTTTTTTTTCGGACCTTGTGAGTCATTTTGATTAATGGTAGCGTCGCTTATCAATGTAAGTTGTTGATACATAATATTTATATATGAtt                                                                                                             <  2:4507709/150‑1 (MQ=255)

                                         gaaCAACGGGCAATAAATAGCCAAATCTTTCTTTATCAAAACGTCGGCACATTGTCGGCGTTTTTTTTCGGACCTTGTGAGTCATTTTGATTAATGGTAGCGTCGCTTGTCAATGTAAGTTGTTGATACATAATATTTATATATGATTaa                                                                                                           <  1:1331770/150‑1 (MQ=255)

                                          aacaacGGGCAATAAATAGCCAAATCTTTCTTTATCAAAACGTCGGCACATTGTCGGCGTTTTTTTTCGGACCGTGTGAGTCATTTTGATTAATGGTAGCGTCGCTTATAAAGGTAAGTTGTTGGTTCATAAAATTTATATATGATTAAt                                                                                                          >  2:4741563/1‑150 (MQ=255)

                                           acaacGGGCAATAAATAGCCAAATCTTTCTTTATCAAAACGTCGGCACATTGTCGGCGTTTTTTTTCGGACCTTGTGAGTCATTTTGATTAATGGTAGCGTCGCTTGTCAATGTAAGTTGTTGATACATAATATTTATATATGATTAATc                                                                                                         <  2:2446394/150‑1 (MQ=255)

                                           acaacGGGCAATAAATAGCCAAATCTTTCTTTATCAAAACGTCGGCACATTGTCGGCGTTTTTTTTCGGACCTTGTGAGTCATTTTGATTAATGGTAGCGTCGCTTGTCAATGTAAGTTGTTGATACATAATATTTATATATGATTAATc                                                                                                         <  1:2060929/150‑1 (MQ=255)

                                            caacGGGCAATAAATAGCCAAATCTTTCTTTATCAAAACGTCGGCACATTGTCGGCGTTTTTTTTAGGGCCATGTGAGTGAATTTGTTTAATGGTGGCGTCGCGGGTGCCAGGGAGTTTTTGATAAAAAAAAATTATAAATGAtttaaaa                                                                                                        >  1:1707735/1‑145 (MQ=255)

                                                gtgaaataaataGCCAAATCTTTCTTTATCAAAACGTCGGCACATTGTCGGCGTTTTTTTTCGGACCTTGTGAGTCATTTTGATTAATGGTAGCGTCGCTTATCAATGTAAGTTGTTGATACATAATATTTATATATGATTAATCAACgg                                                                                                    <  2:3128991/146‑1 (MQ=255)

                                                gggAAATAAATAGCCAAATCTTTCTTTATCAAAACGTCGGCACATTGTCGGCGTTTTTTTTCGGACCTTGTGAGTCATTTTGATTAATGGTAGCGTCGCTTATCAATGTAAGTTGTTGATACATAATATTTATATATGATTAATCAACgg                                                                                                    <  2:3600670/150‑1 (MQ=255)

                                                  gCAATAAATAGCCAAATCTTTCTTTATCAAAACGTCGGCACATTGTCGGCGTTTTTTTTCGGACCTTGTGAGTCATTTTGATTAATGGTAGCGTCGCTTGTCAATGTAAGTTGTTGATACATAATATTTATATATGATTAATCAACGgat                                                                                                  <  2:120462/150‑1 (MQ=255)

                                                  gCAATAAATAGCCAAATCTTTCTTTATCAAAACGTCGGCACATTGTCGGCGTTTTTTTTCGGACCTTGTGAGTCATTTTGATTAATGGTAGCGTCGCTTGTCAATGTAAGTTGTTGATACATAATATTTATATATGATTAATCAACGgat                                                                                                  <  2:2547769/150‑1 (MQ=255)

                                                  gCAATAAATAGCCAAATCTTTCTTTATCAAAACGTCGGCACATTGTCGGCGTTTTTTTTCGGACCTTGTGAGTCATTTTGATTAATGGTAGCGTCGCTTATCAATGTAAGTTGTTGATACATAATATTTATATATGATTAATCAACGgat                                                                                                  <  1:4359952/150‑1 (MQ=255)

                                                  gCAATAAATAGCCAAATCTTTCTTTATCAAAACGTCGGCACATTGTCGGCGTTTTTTTTCGGACCTTGTGAGTCATTTTGATTAATGGTAGCGTCGCTTATCAATGTAAGTTGTTGATACATAATATTTATATATGATTAATCAACGgat                                                                                                  <  1:1527742/150‑1 (MQ=255)

                                                    aataaataGCCAAATCTTTCTTTATCAAAACGTCGGCACATTGTCGGCGTTTTTTTTCGGACCTTGTGAGTCATTTTGATTAATGGTAGCGTCGCTTATCAATGTAAGTTGTTGATACATAATATTTATATATGATTAATCAACGgatga                                                                                                <  2:4608117/150‑1 (MQ=255)

                                                    aataaataGCCAAATCTTTCTTTATCAAAACGTCGGCACATTGTCGGCGTTTTTTTTCGGACCTTGTGAGTCATTTTGATTAATGGTAGCGTCGCTTATCAATGTAAGTTGTTGATACATAATATTTATATATGATTAATCAACGgatga                                                                                                <  2:1113104/150‑1 (MQ=255)

                                                    aataaataGCCAAATCTTTATTTATCAAAACGTCGGCACATTGTCGGCGTTTTTTTTCGGACCTTGTGAGTCATTTTGATTAATGGTAGCGTCGCTTATCAATGTAAGTTGTTGATACATAATATTTATATATGATTAATCAACGgatga                                                                                                <  2:1113109/150‑1 (MQ=255)

                                                     ataaataGCCAAATCTTTCTTTATCAAAACGTCGGCACATTGTCGGCGTTTTTTTTAGGACCTTGGGAGTAATTTTTATTAATGGTAGCGTCGCTTGTGAATTTAAGTTTTTGTTACAAAAAATTTTTTTATTTTTAAAAAAAGgatgtt                                                                                               >  2:3301635/1‑148 (MQ=255)

                                                      taaataGCCAAATCTTTCTTTATCAAAACGTCGGCACATTGTCGGCGTTTTTTTTCGGACCTTGTGAGTCATTTTGATTAATGGTAGCGTCGCTTGTCAATGTAAGTTGTTGATACATAATATTTATATATGATTAATCAACGGATGAtt                                                                                              <  2:4858379/150‑1 (MQ=255)

                                                          taGCCAAATCTTTCTTTATCAAAACGTCGGCACATTGTCGGCGTTTTTTTTCGGACCTTGTGAGTCATTTTGATTAATGGTAGCGTCGCTTGTCAATGTAAGTTGTTGATACATAATATTTATATATGATTAATCAACGGATGATTcaca                                                                                          <  1:1411739/150‑1 (MQ=255)

                                                           aGCCAAATCTTTCTTTATCAAAACGTCGGCACATTGTCGGCGTTTTTTTTCGGACCTTGTGAGTCATTTTGATTAATGGTAGCGTCGCTTGTCAATGTAAGTTGTTGATACATAATATTTATATATGATTAATCAACGGATGATTCACAt                                                                                         <  1:2010262/150‑1 (MQ=255)

                                                            gCCAAATCTTTCTTTATCAAAACGTCGGCACATTGTCGGCGTTTTTTTTCGGACCTTGTGAGTCATTTTGATTAATGGTAGCGTCGCTTGTCAATGTAAGTTGTTGATACATAATATTTATATATGATTAATCAACGGATGATTCACATg                                                                                        <  2:1649498/150‑1 (MQ=255)

                                                                 aTCTTTCTTTATCAAAACGTCGGCACATTGTCGGCGTTTTTTTTCGGACCTTGTGAGTCATTTTGATTAATGGTAGCGTCGCTTGTCAATGTAAGTTGTTGATACATAATATTTATATATGATTAATCAACGGATGATTCACATgaagaa                                                                                   <  2:1654606/150‑1 (MQ=255)

                                                                      tctttATCAAAACGTCGGCACATTGTCGGCGTTTTTTTTCGGTCCTTGGGAGTCATTTTTATTAATGGTAGCGTCGCTTGTCAATGTTAGTTGTTGATACATAATATTTATATATGATTAATAAACGGATGTTTCAAAAGAAGAATaaaa                                                                              >  2:334403/1‑147 (MQ=255)

                                                                      tctttATCAAAACGTCGGCACATTGTCGGCGTTTTTTTTCGGACCTTGTGAGTCATTTTGATTAATGGTAGCGTCGCTTATCAATGTAAGTTGTTGATACATAATATTTATATATGATTAATCAACGGATGATTCACATGAAGAATACTa                                                                              <  1:280270/150‑1 (MQ=255)

                                                                            tCAAAACGTCGGCACATTGTCGGCGTTTTTTTTCGGACCTTTTGAGTCATTTTGATTAATGGTAGCGTCGCTTGTCAATGTAAGTTGTTTTTACATAAAATTTATATATGATTAATCAACGGATGATTAACATGAAGTATAATAAATTAc                                                                        >  2:4031669/1‑150 (MQ=255)

                                                                            tCAAAACGTCGGCACATTGTCGGCGTTTTTTTTCGGACCTTGTGAGTCATTTTGATTAATGGTAGCGTCGCTTGTCAATGTAAGTTGTTGTTAAATAAAATTTATATATTATTAATAAAAGGATGATTAACATGAAGAATACTAAATTaa                                                                        >  2:4032431/1‑149 (MQ=255)

                                                                            tCAAAACGTCGGCACATTGTCGGCGTTTTTTTTCGGACCTTGTGAGTCATTTTGATTAATGGTAGCGTCGCTTGTCAATGTAAGTTGTTGATACATAATATTTATATATGATTAATCAACGGATGATTCACATGAAGAATACTAAATTAc                                                                        <  1:668587/150‑1 (MQ=255)

                                                                            tCAAAACGTCGGCACATTGTCGGCGTTTTTTTTCGGACCTTGTGAGTCATTTTGATTAATGGTAGCGTCGCTTGTCAATGTAAGTTGTTGATACATAATATTTATATATGATTAATCAACGGATGATTCACATGAAGAATACTAAATTAc                                                                        <  1:668659/150‑1 (MQ=255)

                                                                            tCAAAACGTCGGCACATTGTCGGCGTTTTTTTTCGGACCTTGTGAGTCATTTTGATTAATGGTAGCGTCGCTTGTCAATGTAAGTTGTTGATACATAATATTTATATATGATTAATCAACGGATGATTCACATGAAGAATACTAAATTAc                                                                        <  1:668548/150‑1 (MQ=255)

                                                                              aaaaCGTCGGCACATTGTCGGCGTTTTTTTTCGGACCTTGTGAGTCATTTTGATTAATGGTAGCGTCGCTTGTCAATGTAAGTTGTTGATACATAATATTTATATATGATTAATCAACGGATGATTCACATGAAGAATACTAAATTActg                                                                      <  1:1953879/150‑1 (MQ=255)

                                                                                 aCGTCGGCACATTGTCGGCGTTTTTTTTCGGACCTTGTGAGTCATTTTGATTAATGGTAGCGTCGCTTGTCAATGTAAGTTGTTGATACATAATATTTATATATGATTAATCAACGGATGATTCACATGAAGAATACTAAATTActgctg                                                                   <  1:1007591/150‑1 (MQ=255)

                                                                                 aCGTCGGCACATTGTCGGCGTTTTTTTTCGGACCTTGTGAGTCATTTTGATTAATGGTAGCGTCGCTTGTCAATGTAAGTTGTTGATACATAATATTTATATATGATTAATCAACGGATGATTCACATGAAGAATACTAAATTActgctg                                                                   <  1:1007060/150‑1 (MQ=255)

                                                                                  cGTCGGAAATTTGTGGGGTTTTTTTTTCGGACCTTGTGAGTCATTTTGATTAATGGTAGCGTCGCTTGTCAATGTAAGTTGTTGATACATAATATTTATATATGATTAATCAACGGATGATTCACATGAAGAATACTAAATTACTGCTgg                                                                  <  1:3263046/150‑1 (MQ=255)

                                                                                    tCGGCACATTGTCGGCGTTTTTTTTCGGACCTTGTGAGTCATTTTGATTAATGGTAGCGTCGCTTGTCAATGTAAGTTGTTGATACATAATATTTATATATGATTAATCAACGGATGATTCACATGAAGAATACTAAATTACTGCTGGCg                                                                <  2:253591/150‑1 (MQ=255)

                                                                                        cacaTTGTCGGCGTTTTTTTTCGGCCCCTGTGAGTCATTTTGATTAATGGTATCGTCGCGTGTGAAAGTAAGTTTTTGTTACAAAAAATTTATATATGATTAATAAAACGATTATTCAACAGAAAAATAAAAAAATACATCTGGCGaatt                                                            >  2:3742140/1‑147 (MQ=255)

                                                                                        cacaTTGTCGGCGTTTTTTTTCGGACCTTGTGAGTCATTTTGATTAATGGTAGCGTCGCTTGTCAATGTAAGTTGTTGATACATAATATTTATATATGATTAATCAACGGATGATTCACATGAAGAATACTAAATTACTGCTGGCGATTg                                                            <  2:3941582/150‑1 (MQ=255)

                                                                                        cacaTTGTCGGCGTTTTTTTTCGGACCTTGTGAGTCATTTTGATTAATGGTAGCGTCGCTTGTCAATGTAAGTTGTTGATACATAATATTTATATATGATTAATCAACGGATGATTCACATGAAGAATACTAAATTACTGCTGGCGATTg                                                            <  1:375068/150‑1 (MQ=255)

                                                                                        cacaTTGTCGGCGTTTTTTTTCGGACCATGTGTGTAATTTTTATTTATGGTAGCGTCGCTTTTGAAATTAAGTTTTTTTTAAAAAAAATTTTTATTTTATTAATAAAACGGTGATTAAAATTAATAATAAAAAAATAAAGATGGCGAttt                                                            >  2:2967220/1‑149 (MQ=255)

                                                                                          caTTGTCGGCGTTTTTTTTCGGACCTTGTGAGTCATTTTGATTAATGGTAGCGTCGCTTATCAATGTAAGTTGTTGATACATAATATTTATATATGATTAATCAACGGATGATTCACATGAAGAATACTAAATTACTGCTGGCGATTGCg                                                          <  1:2815302/150‑1 (MQ=255)

                                                                                          caTTGTCGGCGTTTTTTTTCGGACCTTGTGAGTCATTTTGATTAATGGTAGCGTCGCTTATCAATGTAAGTTGTTGATACATAATATTTATATATGATTAATCAACGGATGATTCACATGAAGAATACTAAATTACTGCTGGCGATTGCg                                                          <  1:2816055/150‑1 (MQ=255)

                                                                                          caTTGTCGGCGTTTTTTTTCGGACCTTGTGAGTCATTTTGATTAATGGTAGCGTCGCTTATCAATGTAAGTTGTTGATACATAATATTTATATATGATTAATCAACGGATGATTCACATGAAGAATACTAAATTACTGCTGGCGATTGCg                                                          <  1:3613051/150‑1 (MQ=255)

                                                                                                 ggCGTTTTTTTTCGGACCTTGTGAGTCATTTTGATTAATGGTAGCGTCGCTTGTCAATGTAAGTTGTTGATACATAATATTTATATATGATTAATCAACGGATGATTCACATGAAGAATACTAAATTACTGCTGGCGATTGCGACCTCTg                                                   <  2:1743485/150‑1 (MQ=255)

                                                                                                   cGTTTTTTTTCGGACCTTGTGAGTCATTTTGATTAATGGTAGCGTCGCTTGTCAATGTAAGTTGTTGATACATAATATTTATATATGATTAATCAACGGATGATTCACATGAAGAATACTAAATTACTGCTGGCGATTGCGACCTCTgca                                                 <  2:4418950/150‑1 (MQ=255)

                                                                                                   cGTTTTTTTTCGGACCTTGTGAGTCATTTTGATTAATGGTAGCGTCGCTTATCAATGTAAGTTTTTGATAAATAATATTTATATATTATTAATAAACGGATTATTAACATGAAGAATACTAAAATAATTCTGGCGATTTCGACCACTtca                                                 >  1:145876/1‑150 (MQ=255)

                                                                                                        tttttCGGACCTTGTGAGTCATTTTGATTAATGGTAGCGTCGCTTATCAATGTAAGTTGTTGATACATAATATTTATATATGATTAATCAACGGATGATTCACATGAAGAATACTAAATTACTGCTGGCGATTGCGACCTCTGCAGCAtt                                            <  1:2623074/150‑1 (MQ=255)

                                                                                                              ggACCTTGTGAGTCATTTTGATTAATGGTAGCGTCGCTTGTCAATGTAAGTTTTTGATACATAATATTTATATATGATTAATCAACGGATGATTAACATGAAGAATACTAAATTACTGCTGGCGATTGCGACCTCTGCAGCATTAATGAc                                      >  2:29836/1‑150 (MQ=255)

                                                                                                                aCCTTGTGAGTCATTTTGATTAATGGTAGCGTCGCTTATCAATGTAAGTTGTTGATACATAATATTTATATATGATTAATCAACGGATGATTCACATGAAGAATACTAAATTACTGCTGGCGATTGCGACCTCTGCAGCATTACTGACAg                                    <  2:3535041/150‑1 (MQ=255)

                                                                                                                  cTTGTGAGTCATTTTGATTAATGGTAGCGTCGCTTGTCAATGTAAGTTGTTGATACATAATATTTATATATGATTAATCAACGGATGATTCACATGAAGAATACTAAATTACTGCTGGCGATTGCGACCTCTGCAGCATTACTGACAggg                                  >  2:242927/1‑150 (MQ=255)

                                                                                                                    tgtgAGTCATTTTGATTAATGGTAGCGTCGCTTGTCAATGTAAGTTGTTGATACATAATATTTATATATGATTAATCAACGGATGATTCACATGAAGAATACTAAATTACTGCTGGCGATTGCGACCTCTGCAGCATTACTGACAGGgtg                                <  2:3936221/150‑1 (MQ=255)

                                                                                                                    tgtgAGTCATTTTGATTAATGGTAGCGTCGCTTGTCAATGTAAGTTGTTGATACATAATATTTATATATGATTAATCAACGGATGATTCACATGAAGAATACTAAATTACTGCTGGCGATTGCGACCTCTGCAGCATTACTGACAGGgtg                                <  2:3937306/150‑1 (MQ=255)

                                                                                                                       gAGTCATTTTGATTAATGGTAGCGTCGCTTGTCAATGTAAGTTGTTGATACATAATATTTATATATGATTAATCAACGGATGATTCACATGAAGAATACTAAATTACTGCTGGCGATTGCGACCTCTGCAGCATTACTGACAGGGTGTCa                             <  2:4687726/150‑1 (MQ=255)

                                                                                                                       gAGTCATTTTGATTAATGGTAGCGTCGCTTGTCAATGTAAGTTGTTGATACATAATATTTATATATGATTAATCAACGGATGATTCACATGAAGAATACTAAATTACTGCTGGCGATTGCGACCTCTGCAGCATTACTGACAGGGTGTCa                             <  1:1297502/150‑1 (MQ=255)

                                                                                                                       gAGTCATTTTGATTAATGGTAGCGTCGCTTGTCAATGTAAGTTGTTGATACATAATATTTATATATGATTAATCAACGGATGATTCACATGAAGAATACTAAATTACTGCTGGCGATTGCGACCTCTGCAGCATTACTGACAGGGTGTCa                             <  1:1758856/150‑1 (MQ=255)

                                                                                                                       gAGTCATTTTGATTAATGGTAGCGTCGCTTGTCAATGTAAGTTGTTGATACATAATATTTATATATGATTAATCAACGGATGATTCACATGAAGAATACTAAATTACTGCTGGCGATTGCGACCTCTGCAGCATTACTGACAGGGTGTCa                             <  1:4213158/150‑1 (MQ=255)

                                                                                                                           cATTTTGATTAATGGTAGCGTCGCTTATCAATGTAAGTTGTTGATACATAATATTTATATATTATTAATCAACGGATGATTCACATGAAGAATACTAAATTACTGCTGGCGATTTCGACCTCTGCAGCATTACTGACAGGGTGTCAAAAt                         >  2:389040/1‑150 (MQ=255)

                                                                                                                            aTTTTGATTAATGGTAGCGTCGCTTGTCAATGTAAGTTGTTGATACATAATATTTATATATGATTAATCAACGGATGATTCACATGAAGAATACTAAATTACTGCTGGCGATTGCGACCTCTGCAGCATTACTGACAGGGTGTCAAAATa                        <  2:555557/150‑1 (MQ=255)

                                                                                                                            aTTTTGATTAATGGTAGCGTCGCTTGTCAATGTAAGTTGTTGATACATAATATTTATATATGATTAATAAACGGATGATTAACATGAAGAATACTAAATTACTTCTGGCGATTTCGACCTCTGCAGCATTACTGACAGGGTGTCAAAATa                        >  1:4827489/1‑150 (MQ=255)

                                                                                                                                tGATTAATGGTAGCGTCGCTTGTCAATGTAAGTTGTTGATACATAATATTTATATATGATTAATCAACGGATGATTCACATGAAGAATACTAAATTACTTCTGGCGATTGCGACCTCTGCAGCATTACTGACAGGGTGTCAAAATAACCa                    >  2:4783494/1‑150 (MQ=255)

                                                                                                                                        ggTAGCGTCGCTTATCAATGTAAGTTGTTGATACATAATATTTATATATGATTAATCAACGGATGATTCACATGAAGAATACTAAATTACTGCTGGCGATTGCGACCTCTGCAGCATTACTGACAGGGTGTCAAAATACCCACGGTATTg            <  1:4341631/150‑1 (MQ=255)

                                                                                                                                           aGCGTCGCTTATCAATGTAAGTTGTTGATACATAATATTTATATATGATTAATCAACGGATGATTCACATGAAGAATACTAAATTACTGCTGGCGATTGCGACCTCTGCAGCATTACTGACAGGGTGTCAAAATACCCACGGTATTGATa         <  1:2787606/150‑1 (MQ=255)

                                                                                                                                            gCGTCGCTTGTCAATGTAAGTTGTTGATACATAATATTTATATATGATTAATCAACGGATGATTCACATGAAGAATACTAAATTACTGCTGGCGATTGCGACCTCAGCAGCATTACTGACAGGGTGTCAAAATACCCACGGTATTGATAc        >  2:3335717/1‑150 (MQ=255)

                                                                                                                                             cGTCGCTTGTCAATGTAAGTTGTTGATACATAATATTTATATATGATTAATCAACGGATGATTCACATGAAGAATACTAAATTACTGCTGGCGATTGCGACCTCTGCAGCATTACTGACAGGGTGTCAAAATACCCACGGTATTGATAcc       >  2:3873811/1‑150 (MQ=255)

                                                                                                                                             cGTCGCTTGTCAATGTAAGTTGTTGATACATAATATTTATATATGATTAATCAACGGATGATTCACATGAAGAATACTAAATTACTGCTGGCGATTGCGACCTCTGCAGCATTACTGACAGGGTGTCAAAATACCCACGGTATTGATAcc       <  2:4802629/150‑1 (MQ=255)

                                                                                                                                              gTCGCTTGTCAATGTAAGTTGTTGATACATAATATTTATATATGATTAATCAACGGATGATTCACATGAAGAATACTAAATTACTGCTGGCGATTGCGACCTCTGCAGCATTACTGACAGGGTGTCAAAATACCCACGGTATTGATACCa      <  2:149345/150‑1 (MQ=255)

                                                                                                                                               tCGCTTGTCAATGTAAGTTGTTGATACATAATATTTATATATGATTAATCAACGGATGATTCACATGAAGAATACTAAATTACTGCTGGCGATTGCGACCTCTGCAGCATTACTGACAGGGTGTCAAAATACCCACGGTATTGATACCaa     >  1:3624737/1‑150 (MQ=255)

                                                                                                                                               tCGCTTATCAATGTAAGTTGTTGATACATAATATTTATATATGATTAATCAACGGATGATTCACATGAAGAATACTAAATTACTGCTGGCGATTGCGACCTCTGCAGCATTACTGACAGGGTGTCAAAATACCCACGGTATTGATACCaa     <  1:1238383/150‑1 (MQ=255)

                                                                                                                                                  cTTGTCAATGTAAGTTGTTGATACATAATATTTATATATGATTAATCAACGGATGATTCACATGAAGAATACTAAATTACTGCTGGCGATTGCGACCTCTGCAGCATTACTGACAGGGTGTCAAAATACCCACGGTATTGATACCAatat  >  2:3123191/1‑150 (MQ=255)

TATTTCGAGCAGCTGGCGCGGATTAGCCAGGCAGCCTGAATGAACAACGGGCAATAAATAGCCAAATCTTTCTTTATCAAAACGTCGGCACATTGTCGGCGTTTTTTTTCGGACCTTGTGAGTCATTTTGATTAATGGTAGCGTCGCTTGTCAATGTAAGTTGTTGATACATAATATTTATATATGATTAATCAACGGATGATTCACATGAAGAATACTAAATTACTGCTGGCGATTGCGACCTCTGCAGCATTACTGACAGGGTGTCAAAATACCCACGGTATTGATACCAATAT  >  CP009273/955513‑955808

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A