Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F98 I0 R1	799	294.1	9752082	92.2%	8991419	150.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	1,338,978	G→A	21.6%	intergenic (‑112/+36)	yciZ ← / ← gmr	uncharacterized protein/cyclic‑di‑GMP phosphodiesterase; csgD regulator; modulator of Rnase II stability

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	1,338,978	0	G	A	21.6%	467.7 / 124.2	208	intergenic (‑112/+36)	yciZ/gmr	uncharacterized protein/cyclic‑di‑GMP phosphodiesterase; csgD regulator; modulator of Rnase II stability
Reads supporting (aligned to +/- strand): ref base G (97/66); new base A (32/13); total (129/79)
Fisher's exact test for biased strand distribution p-value = 1.69e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.63e-01

Only 100 of 208 total aligned reads displayed.

TACGTTCGGCAACGCGCTGGGCATCAAATTCAGACATCAAGGACATCCTTATAGCAAAAGGGGGAAAATGAATAATGCAAAAATTGCCGCTAAAAGAGAAGTGTTTAACAGCAACGGCTAATTATCATCCAGGAATACGATATATAATGACGGGATATAGCGCTAAGTATATATATTCATCTACTTATGCGCGCTTCAGATAGCGTTTATACCAGCGTTCGAAGGCGACGGCGGGCATCGGTTTGGCAAACAAAAATCCTTGCCGCTCATTGATCCCGTTCTTGGTTAAAAAAGCAT  >  CP009273/1338830‑1339126

tACGTTCGGCAACGCGCTGGGCATCAAATTCAGACATCAAGGACATCCTTATAGCAAAAGGGGGAAAATGAATAATGCAAAAATTGCCGCTAAAAGAGAAGTGTTTAACAGCAACGGCTAATTATCATCCAGGAATACGATATATAATGa                                                                                                                                                     <  1:3288974/150‑1 (MQ=255)

  cGTTCGGCAACGCGCTGGGCATCAAATTCAGACATCAAGGACATCCTTATAGCAAAAGGGGGAAAATGAATAATGCAAAAATTGCCGCTAAAAGAGAAGTGTTTAACAGCAACGGCTAATTATCATCCAGGAATACGATATATAATGACg                                                                                                                                                   <  1:1148731/150‑1 (MQ=255)

  cGTTCGGCAACGCGCTGGGCATCAAATTCAGACATCAAGGACATCCTTATAGCAAAAGGGGGAAAATGAATAATGCAAAAATTGCCGCTAAAAGAGAAGTGTTTAACAGCAACGGCTAATTATCATCCAGGAATACGATATATAATGACg                                                                                                                                                   <  1:2872756/150‑1 (MQ=255)

      cGGCAACGCGCTGGGCATCAAATTCAGACATCAAGGACATCCTTATAGCAAAAGGGGGAAAATGAATAATGCAAAAATTGCCGCTAAAAGAGAAGTGTTTAACAGCAACGGCTAATTATCATCCAGGAATACGATATATAATGACGGGat                                                                                                                                               >  2:917609/1‑150 (MQ=255)

      cGGCAACGCGCTGGGCATCAAATTCAGACATCAAGGACATCCTTATAGCAAAAGGGGGAAAATGAATAATGCAAAAATTGCCGCTAAAAGAGAAGTGTTTAACAGCAACGGCTAATTATCATCCAGGAATACGATATATAATGACGGGat                                                                                                                                               >  2:4339112/1‑150 (MQ=255)

         cAACGCGCTGGGCATCAAATTCAGACATCAAGGACATCCTTATAGCAAAAGGGGGAAAATGAATAATGCAAAAATTGCCGCTAAAAGAGAAGTGTTTAACAGCAACGGCTAATTATCATCCAGGAATACGATATATAATGACGGGAtata                                                                                                                                            >  1:3319766/1‑150 (MQ=255)

         cAACGCGCTGGGCATCAAATTCAGACATCAAGGACATCCTTATAGCAAAAGGGGGAAAATGAATAATGCAAAAATTGCCGCTAAAAGAGAAGTGTTTAACAGCAACGGCTAATTATCATCCAGGAATACGATATATAATGACGGGAtata                                                                                                                                            >  1:3636804/1‑150 (MQ=255)

          aaCGCGCTGGGCATCAAATTCAGACATCAAGGACATCCTTATAGCAAAAGGGGGAAAATGAATAATGCAAAAATTGCCGCTAAAAGAGAAGTGTTTAACAGCAACGGCTAATTATCATCCAGGAATACGATATATAATGACGGGATATAg                                                                                                                                           >  2:3778856/1‑150 (MQ=255)

              cgcTGGGCATCAAATTCAGACATCAAGGACATCCTTATAGCAAAAGGGGGAAAATGAATAATGCAAAAATTGCCGCTAAAAGAGAAGTGTTTAACAGCAACGGCTAATTATCATCCAGGAATACGATATATAATAACGGGATATAGCGCt                                                                                                                                       <  2:462940/150‑1 (MQ=255)

                 tGGGCATCAAATTCAGACATCAAGGACATCCTTATAGCAAAAGGGGGAAAATGAATAATGCAAAAATTGCCGCTAAAAGAGAAGTGTTTAACAGCAACGGCTAATTATCATCCAGGAATACGATATATAATAACGGGATATAGCGCTAAg                                                                                                                                    <  2:1141377/150‑1 (MQ=255)

                 tGGGCATCAAATTCAGACATCAAGGACATCCTTATAGCAAAAGGGGGAAAATGAATAATGCAAAAATTGCCGCTAAAAGAGAAGTGTTTAACAGCAACGGCTAATTATCATCCAGGAATACGATATATAATAACGGGATATAGCGCTAAg                                                                                                                                    <  1:857749/150‑1 (MQ=255)

                  gggCATCAAATTCAGACATCAAGGACATCCTTATAGCAAAAGGGGGAAAATGAATAATGCAAAAATTGCCGCTAAAAGAGAAGTGTTTAACAGCAACGGCTAATTATCATCCAGGAATACGATATATAATGACGGGATATAGCGCTAAGt                                                                                                                                   >  1:3363649/1‑150 (MQ=255)

                  gggCATCAAATTCAGACATCAAGGACATCCTTATAGCAAAAGGGGGAAAATGAATAATGCAAAAATTGCCGCTAAAAGAGAAGTGTTTAACAGCAACGGCTAATTATCATCCAGGAATACGATATATAATGACGGGATATAGCGCTAAGt                                                                                                                                   <  1:2424868/150‑1 (MQ=255)

                    gCATCAAATTCAGACATCAAGGACATCCTTATAGCAAAAGGGGGAAAATGAATAATGCAAAAATTGCCGCTAAAAGAGAAGTGTTTAACAGCAACGGCTAATTATCATCCAGGAATACGATATATAATAACGGGATATAGCGCTAAGtat                                                                                                                                 <  1:445792/150‑1 (MQ=255)

                     cATCAAATTCAGACATCAAGGACATCCTTATAGCAAAAGGGGGAAAATGAATAATGCAAAAATTGCCGCTAAAAGAGAAGTGTTTAACAGCAACGGCTAATTATCATCCAGGAATACGATATATAATGACGGGATATAGCGCTAAGtata                                                                                                                                <  2:547509/150‑1 (MQ=255)

                      aTCAAATTCAGACATCAAGGACATCCTTATAGCAAAAGGGGGAAAATGAATAATGCAAAAATTGCCGCTAAAAGAGAAGTGTTTAACAGCAACGGCTAATTATCATCCAGGAATACGATATATAATGACGGGATATAGCGCTAAGtatat                                                                                                                               >  2:2166035/1‑150 (MQ=255)

                       tCAAATTCAGACATCAAGGACATCCTTATAGCAAAAGGGGGAAAATGAATAATGCAAAAATTGCCGCTAAAAGAGAAGTGTTTAACAGCAACGGCTAATTATCATCCAGGAATACGATATATAATGACGGGATATAGCGCTAAGtatata                                                                                                                              <  2:2278068/150‑1 (MQ=255)

                         aaaTTCAGACATCAAGGACATCCTTATAGCAAAAGGGGGAAAATGAATAATGCAAAAATTGCCGCTAAAAGAGAAGTGTTTAACAGCAACGGCTAATTATCATCCAGGAATACGATATATAATGACGGGATATAGCGCTAAGtatatata                                                                                                                            >  2:2083324/1‑150 (MQ=255)

                         aaaTTCAGACATCAAGGACATCCTTATAGCAAAAGGGGGAAAATGAATAATGCAAAAATTGCCGCTAAAAGAGAAGTGTTTAACAGCAACGGCTAATTATCATCCAGGAATACGATATATAATAACGGGATATAGCGCTAAGtatatata                                                                                                                            >  1:2654132/1‑150 (MQ=255)

                         aaaTTCAGACATCAAGGACATCCTTATAGCAAAAGGGGGAAAATGAATAATGCAAAAATTGCCGCTAAAAGAGAAGTGTTTAACAGCAACGGCTAATTATCATCCAGGAATACGATATATAATAACGGGATATAACGCTAAGtatatata                                                                                                                            >  2:1885518/1‑150 (MQ=255)

                          aaTTCAGACATCAAGGACATCCTTATAGCAAAAGGGGGAAAATGAATAATGCAAAAATTGCCGCTAAAAGAGAAGTGTTTAACAGCAACGGCTAATTATCATCCAGGAATACGATATATAATGACGGGATATAGCGCTAAGTatatatat                                                                                                                           >  1:4501362/1‑150 (MQ=255)

                                gACATCAAGGACATCCTTATAGCAAAAGGGGGAAAATGAATAATGCAAAAATTGCCGCTAAAAGAGAAGTGTTTAACAGCAACGGCTAATTATCATCCAGGAATACGATATATAATGACGGGATATAGCGCTAAGTATATATATTCATCt                                                                                                                     >  1:2274425/1‑150 (MQ=255)

                                 aCATCAAGGACATCCTTATAGCAAAAGGGGGAAAATGAATAATGCAAAAATTGCCGCTAAAAGAGAAGTGTTTAACAGCAACGGCTAATTATCATCCAGGAATACGATATATAATGACGGGATATAGCGCTAAGTATATATATTCATCTa                                                                                                                    <  2:298958/150‑1 (MQ=255)

                                 aCATCAAGGACATCCTTATAGCAAAAGGGGGAAAATGAATAATGCAAAAATTGCCGCTAAAAGAGAAGTGTTTAACAGCAACGGCTAATTATCATCCAGGAATACGATATATAATGACGGGATATAGCGCTAAGTATATATATTCATCTa                                                                                                                    <  2:16634/150‑1 (MQ=255)

                                  cATCAAGGACATCCTTATAGCAAAAGGGGGAAAATGAATAATGCAAAAATTGCCGCTAAAAGAGAAGTGTTTAACAGCAACGGCTAATTATCATCCAGGACTACGATATATAATGACGGGATATAGCGCTAAGGCTCTATATTCATCTAc                                                                                                                   >  2:45155/1‑150 (MQ=255)

                                  cATCAAGGACATCCTTATAGCAAAAGGGGGAAAATGAATAATGCAAAAATTGCCGCTAAAAGAGAAGTGTTTAACAGCAACGGCTAATTATCATCCAGGAATACGATATATAATGACGGGATATAGCGCTAAGTATATATATTCATCTAc                                                                                                                   <  2:3655520/150‑1 (MQ=255)

                                         gACATCCTTATATCAAAAGGGGGAAAATGAATAATGCAAAAATTGCCGCTAAAAGAGAAGTGTTTAACAGCAACGGCTAATTATCATCCAGGAATACGATATATAATGACGGGATATAGCGCTAAGTATATATATTCATCTACTTATgcg                                                                                                            <  2:389656/150‑1 (MQ=255)

                                           cATCCTTATAGCAAAAGGGGGAAAATGAATAATGCAAAAATTGCCGCTAAAAGAGAAGTGTTTAACAGCAACGGCTAATTATCATCCAGGAATACGATATATAATGACGGGATATAGCGCTAAGTATATATATTCATCTACTTATgcgcg                                                                                                          >  1:4797930/1‑150 (MQ=255)

                                              ccTTATAGCAAAAGGGGGAAAATGAATAATGCAAAAATTGCCGCTAAAAGAGAAGTGTTTAACAGCAACGGCTAATTATCATCCAGGAATACGATATATAATGACGGGATATAGCGCTAAGTATATATATTCATCTACTTATGCGCGCtt                                                                                                       <  2:1795509/150‑1 (MQ=255)

                                              ccTTATAGCAAAAGGGGGAAAATGAATAATGCAAAAATTGCCGCTAAAAGAGAAGTGTTTAACAGCAACGGCTAATTATCATCCAGGAATACGATATATAATGACGGGATATAGCGCTAAGTATATATATTCATCTACTTATGCGCGCtt                                                                                                       >  2:3918364/1‑150 (MQ=255)

                                                ttATAGCAAAAGGGGGAAAATGAATAATGCAAAAATTGCCGCTAAAAGAGAAGTGTTTAACAGCAACGGCTAATTATCATCCAGGAATACGATATATAATGACGGGATATAGCGCTAAGTATATATATTCATCTACTTATGCGCGCTTCa                                                                                                     <  1:2363090/150‑1 (MQ=255)

                                                ttATAGCAAAAGGGGGAAAATGAATAATGCAAAAATTGCCGCTAAAAGAGAAGTGTTTAACAGCAACGGCTAATTATCATCCAGGAATACGATATATAATGACGGGATATAGCGCTAAGTATATATATTCATCTACTTATGCGCGCTTCa                                                                                                     >  2:3760994/1‑150 (MQ=255)

                                                ttATAGCAAAAGGGGGAAAATGAATAATGCAAAAATTGCCGCTAAAAGAGAAGTGTTTAACAGCAACGGCTAATTATCATCCAGGAATACGATATATAATGACGGGATATAGCGCTAAGTATATATATTCATCTACTTATGCGCGCTTCa                                                                                                     >  1:1244116/1‑150 (MQ=255)

                                                  ataGCAAAAGGGGGAAAATGAATAATGCAAAAATTGCCGCTAAAAGAGAAGTGTTTAACAGCAACGGCTAATTATCATCCAGGAATACGATATATAATGACGGGATATAGCGCTAAGTATATATATTCATCTACTTATGCGCGCTTCAGa                                                                                                   <  1:4403851/150‑1 (MQ=255)

                                                  ataGCAAAAGGGGGAAAATGAATAATGCAAAAATTGCCGCTAAAAGAGAAGTGTTTAACAGCAACGGCTAATTATCATCCAGGAATACGATATATAATAACGGGATATAGCGCTAAGTCTATATATTCATCTACTTATGCGCGCTTCAGa                                                                                                   >  1:3858726/1‑150 (MQ=255)

                                                  ataGCAAAAGGGGGAAAATGAATAATGCAAAAATTGCCGCTAAAAGAGAAGTGTTTAACAGCAACGGCTAATTATCATCCAGGAATACGATATATAATAACGGGATATAGCGCTAAGTATATATATTCATCTACTTATGCGCGCTTCAGa                                                                                                   >  1:3483390/1‑150 (MQ=255)

                                                        aaaGGGGGAAAATGAATAATGCAAAAATTGCCGCTAAAAGAGAAGTGTTTAACAGCAACGGCTAATTATCATCCAGGAATACGATATATAATGACGGGATATAGCGCTAAGTATATATATTCATCTACTTATGCGCGCTTCAGATAGCGt                                                                                             >  2:679679/1‑150 (MQ=255)

                                                           gggggAAAATGAATAATGCAAAAATTGCCGCTAAAAGAGAAGTGTTTAACATCAACGGCTAATTATCATCCAGGAATACGATATATAATGACGGGATATAGCGCTAAGTATATATATTCATCTACTTATGCGCGCTTCAGATAGCGTTta                                                                                          >  1:4023504/1‑150 (MQ=255)

                                                           gggggAAAATGAATAATGCAAAAATTGCCGCTAAAAGAGAAGTGTTTAACAGCAACGGCTAATTATCATCCAGGAATACGATATATAATGACGGGATATAGCGCTAAGTATATATATTCATCTACTTATGCGCGCTTCAGATAGCGTTta                                                                                          >  1:689815/1‑150 (MQ=255)

                                                              ggAAAATGAATAATGCAAAAATTGCCGCTAAAAGAGAAGTGTTTAACAGCAACGGCTAATTATCATCCAGGAATACGATATATAATGACGGGATATAGCGCTAAGTATATATATTCATCTACTTATGCGCGCTTCAGATAGCGTTTATAc                                                                                       >  1:2488746/1‑150 (MQ=255)

                                                               gAAAATGAATAATGCAAAAATTGCCGCTAAAAGAGAAGTGTTTAACAGCAACGGCTAATTATCATCCAGGAATACGATATATAATAACGGGATATAGCGCTAAGTATATATATTCATCTACTTATGCGCGCTTCAGATAGCGTTTATAcc                                                                                      <  2:1775959/150‑1 (MQ=255)

                                                                aaaaTGAATAATGCAAAAATTGCCGCTAAAAGAGAAGTGTTTAACAGCAACGGCTAATTATCATCCAGGAATACGATATATAATAACGGGATATAGCGCTAAGTATATATATTCATCTACTTATGCGCGCTTCAGATAGCGTTTATACCa                                                                                     >  2:3706492/1‑150 (MQ=255)

                                                                aaaaTGAATAATCCAAAAATTGCCGCTAAAAGAGAAGTGTTTAACAGCAACGGCTAATTATCATCCAGGAATACGATATATAATGACGGGATATAGCGCTAAGTATATATATTCATCTACTTATGCGCGCTTCAGATAGCGTTTATACCa                                                                                     <  2:4152300/150‑1 (MQ=255)

                                                                     gAATAATGCAAAAATTGCCGCTAAAAGAGAAGTGTTTAACAGCAACGGCTAATTATCATCCAGGAATACGATATATAATAACGGGATATAGCGCTAAGTATATATATTCATCTACTTATGCGCGCTTCAGATAGCGTTTATACCAGCGtt                                                                                <  1:2826699/150‑1 (MQ=255)

                                                                      aataatGCAAAAATTGCCGCTAAAAGAGAAGTGTTTAACAGCAACGGCTAATTATCATCCAGGAATACGATATATAATGACGGGATATAGCGCTAAGTCTATATATTCATCTACTTATGCGCGCTTCAGATAGCGTTTATACCAGCGTTc                                                                               >  1:2921848/1‑150 (MQ=255)

                                                                            gCAAAAATTGCCGCTAAAAGAGAAGTGTTTAACAGCAACGGCTAATTATCATCCAGGAATACGATATATAATGACGGGATATAGCGCTAAGTATATATATTCATCTACTTATGCGCGCTTCAGATAGCGTTTATACCAGCGTTCGAAGGc                                                                         >  1:1290727/1‑150 (MQ=255)

                                                                            gCAAAAATTGCCGCTAAAAGAGAAGTGTTTAACAGCAACGGCTAATTATCATCCAGGAATACGATATATAATGACGGGATATAGCGCTAAGTATATATATTCATCTACTTATGCGCGCTTCAGATAGCGTTTATACCAGCGTTCGAAGGc                                                                         >  1:609923/1‑150 (MQ=255)

                                                                            gCAAAAATTGCCGCTAAAAGAGAAGTGTTTAACAGCAACGGCTAATTATCATCCAGGAATACGATATATAATGACGGGATATAGCGCTAAGTATATATATTCATCTACTTATGCGCGCTTCAGATAGCGTTTATACCAGCGTTCGAAGGc                                                                         >  1:2257324/1‑150 (MQ=255)

                                                                             cAAAAATTGCCGCTAAAAGAGAAGTGTTTAACAGCAACGGCTAATTATCATCCAGGAATACGATATATAATGACGGGATATAGCGCTAAGTATATATATTCATCTACTTATGCGCGCTTCAGATAGCGTTTATACCAGCGTTCGAAGGCg                                                                        >  2:3589710/1‑150 (MQ=255)

                                                                               aaaaTTGCCGCTAAAAGAGAAGTGTTTAACAGCAACGGCTAATTATCATCCAGGAATACGATATATAATGACGGGATATAGCGCTAAGTATATATATTCATCTACTTATGCGCGCTTCAGATAGCGTTTATACCAGCGTTCGAAGGCGAc                                                                      >  2:1567755/1‑150 (MQ=255)

                                                                               aaaaTTGCCGCTAAAAGAGAAGTGTTTAACAGCAACGGCTAATTATCATCCAGGAATACGATATATAATAACGGGATATAGCGCTAAGTATATATATTCATCTACTTATGCGCGCTTCAGATAGCGTTTATACCAGCGTTCGAAGGCGAc                                                                      >  1:4166897/1‑150 (MQ=255)

                                                                                  aTTGCCGCTAAAAGAGAAGTGTTTAACAGCAACGGCTAATTATCATCCAGGAATACGATATATAATGACGGGATATAGCGCTAAGTATATATATTCATCTACTTATGCGCGCTTCAGATAGCGTTTATACCAGCGCTCGAAGGCGAcggc                                                                   <  2:3801847/150‑1 (MQ=255)

                                                                                   ttGCCGCTAAAAGAGAAGTGTTTAACAGCAACGGCTAATTATCATCCAGGAATACGATATATAATGACGGGATATAGCGCTAAGTATATATATTCATCTACTTATGCGCGCTTCAGATAGCGTTTATACCAGCGTTCGAAGGCGAcggcg                                                                  >  2:320474/1‑150 (MQ=255)

                                                                                   ttGCCGCTAAAAGAGAAGTGTTTAACAGCAACGGCTAATTATCATCCAGGAATACGATATATAATGACGGGATATAGCGCTAAGTATATATATTCATCTACTTATGCGCGCTTCAGATAGCGTTTATACCAGCGTTCGAAGGCGAcggcg                                                                  >  2:556877/1‑150 (MQ=255)

                                                                                        gCTAAAAGAGAAGTGTTTAACAGCAACGGCTAATTATCATCCAGGAATACGATATATAATGACGGGATATAGCGCTAAGTATATATATTCATCTACTTATGCGCGCTTCAGATAGCGTTTATACCAGCGTTCGAAGGCGACGGCGGGCAt                                                             <  1:586462/150‑1 (MQ=255)

                                                                                         cTAAAAGAGAAGTGTTTAACAGCAACGGCTAATTATCATCCAGGAATACGATATATAATGACGGGATATAGCGCTAAGTATATATATTCATCTACTTATGCGCGCTTCAGATAGCGTTTATACCAGCGTTCGAAGGCGACGGCGGGCATc                                                            >  2:1574546/1‑150 (MQ=255)

                                                                                          tAAAAGAGAAGTGTTTAACAGCAACGGCTAATTATCATCCAGGAATACGATATATAATGACGGGATATAGCGCTAAGTATATATATTCATCTACTTATGCGCGCTTCAGATAGCGTTTATACCAGCGTTCGAAGGCGACGGCGGGCATCg                                                           <  2:4263674/150‑1 (MQ=255)

                                                                                            aaaGAGAAGTGTTTAACAGCAACGGCTAATTATCATCCAGGAATACGATATATAATGACGGGATATAGCGCTAAGTATATATATTCATCTACTTATGCGCGCTTCAGATAGCGTTTATACCAGCGTTCGAAGGCGACGGCGGGCATCGGt                                                         >  2:921231/1‑150 (MQ=255)

                                                                                            aaaGAGAAGTGTTTAACAGCAACGGCTAATTATCATCCAGGAATACGATATATAATGACGGGATATAGCGCTAAGTATATATATTCATCTACTTATGCGCGCTTCAGATAGCGTTTATACCAGCGTTCGAAGGCGACGGCGGGCATCGGt                                                         >  2:3362059/1‑150 (MQ=255)

                                                                                            aaaGAGAAGTGTTTAACAGCAACGGCTAATTATCATCCAGGAATACGATATATAATGACGGGATATAGCGCTAAGTATATATATTCATCTACTTATGCGCGCTTCAGATAGCGTTTATACCAGCGTTCGAAGGCGACGGCGGGCATCGGt                                                         >  2:3520523/1‑150 (MQ=255)

                                                                                            aaaGAGAAGTGTTTAACAGCAACGGCTAATTATCATCCAGGAATACGATATATAATGACGGGATATAGCGCTAAGTATATATATTCATCTACTTATGCGCGCTTCAGATAGCGTTTATACCAGCGTTCGAAGGCGACGGCGGGCATCGGt                                                         >  1:2565234/1‑150 (MQ=255)

                                                                                              agagAAGTGTTTAACAGCAACGGCTAATTATCATCCAGGAATACGATATATAATGACGGGATATAGCGCTAAGTATATATATTCATCTACTTATGCGCGCTTCAGATAGCGTTTATACCCGCGTTCGAAGGCGACGGCGGGCATCGGttt                                                       >  2:1357286/1‑150 (MQ=255)

                                                                                               gagaAGTGTTTAACAGCAACGGCTAATTATCATCCAGGAATACGATATATAATGACGGGATATAGCGCTAAGTATATATATTCATCTACTTATGCGCGCTTCAGATAGCGTTTATACCAGCGTTCGAAGGCGACGGCGGGCATCGGTTTg                                                      <  2:2967720/150‑1 (MQ=255)

                                                                                               gagaAGTGTTTAACAGCAACGGCTAATTATCATCCAGGAATACGATATATAATGACGGGATATAGCGCTAAGTATATATATTCATCTACTTATGCGCGCTTCAGATAGCGTTTATACCAGCGTTCGAAGGCGACGGCGGGCATCGGTTTg                                                      >  1:4303310/1‑150 (MQ=255)

                                                                                                agaAGTGTTTAACAGCAACGGCTAATTATCATCCAGGAATACGATATATAATGACGGGATATAGCGCTAAGTATATATATTCATCTACTTATGCGCGCTTCAGATAGCGTTTATACCAGCGTTCGAAGGCGACGGCGGGCATCGGTTTgg                                                     >  1:2355902/1‑150 (MQ=255)

                                                                                                     tgtTTAACAGCAACGGCTAATTATCATCCAGGAATACGATATATAATAACGGGATATAGCGCTAAGTATATATATTCATCTACTTATGCGCGCTTCAGATAGCGTTTATACCAGCGTTCGAAGGCGACGGCGGGCATCGGTTTGGcaaac                                                <  1:3133870/150‑1 (MQ=255)

                                                                                                           aCAGCAACGGCTAATTATCATCCAGGAATACGATATATAATAACGGGATATAGCGCTAAGTATATATATTCATCTACTTATGCGCGCTTCAGATAGCGTTTATACCAGCGTTCGAAGGCGACGGCGGGCATCGGTTTGGCAAACAAAAAt                                          <  1:1851420/150‑1 (MQ=255)

                                                                                                             aGCAACGGCTAATTATCATCCAGGAATACGATATATAATGACGGGATATAGCGCTAAGTATATATATTCATCTACTTATGCGCGCTTCAGATAGCGTTTATACCAGCGTTCGAAGGCGACGGCGGGCATCGGTTTGGCAAACAAAAATcc                                        >  2:1067522/1‑150 (MQ=255)

                                                                                                             aGCAACGGCTAATTATCATCCAGGAATACGATATATAATGACGGGATATAGCGCTAAGTATATATATTCATCTACTTATGCGCGCTTCAGATAGCGTTTATACCAGCGTTCGAAGGCGACGGCGGGCATCGGTTTGGCAAACAAAAATcc                                        <  1:3564694/150‑1 (MQ=255)

                                                                                                             aGCAACGGCTAATTATCATCCAGGAATACGATATATAATAACGGGATATAGCGCTAAGTATATATATTCATCTACTTATGCGCGCTTCAGATAGCGTTTATACCAGCGTTCGAAGGCGACGGCGGGCATCGGTTTGGCAAACAAAAATcc                                        >  1:474682/1‑150 (MQ=255)

                                                                                                             aGCAACGGATTATTATCATACATGAATTCGATATATAATGACGGGATATATCGATAAGTATATATATTCATATACTTTTGCGCGCTTCATATAGCGTTTATACCAGCGTTCGAAGTCGACTTCGGGCATCGGTTTGGCAAACAAAAATcc                                        <  1:1110219/150‑1 (MQ=255)

                                                                                                               cAACGGCTAATTATCATCCAGGAATACGATATATAATGACGGGATATAGCGCTAAGTATATATATTCATCTACTTATGCGCGCTTCAGATAGCGTTTATACCAGCGTTCGAAGGCGACGGCGGGCATCGGTTTGGCAAACAAAAATCCtt                                      >  1:2155599/1‑150 (MQ=255)

                                                                                                                aaCGGCTAATTATCATCCAGGAATACGATATATAATGACGGGATATAGCGCTAAGTATATATATTCATCTACTTATGCGCGCTTCAGATAGCGTTTATACCAGCGTTCGAAGGCGACGGCGGGCATCGGTTTGGCAAACAAAAATCCTTg                                     >  2:2374315/1‑150 (MQ=255)

                                                                                                                aaCGGCTAATTATCATCCAGGAATACGATATATAATAACGGGATATAGCGCTAAGTATATATATTCATCTACTTATGCGCGCTTCAGATAGCGTTTATACCAGCGTTCGAAGGCGACGGCGGGCATCGGTTTGGCAAACAAAAATCCTTg                                     >  1:3132568/1‑150 (MQ=255)

                                                                                                                 aCGGCTAATTATCATCCAGGAATACGATATATAATGACGGGATATAGCGCTAAGTATATATATTCATCTACTTATGCGCGCTTCAGATAGCGTTTATACCAGCGTTCGAAGGCGACGGCGGGCATCGGTTTGGCAAACAAAAATCCTTGc                                    >  1:63609/1‑150 (MQ=255)

                                                                                                                  cGGCTAATTATCATCCAGGAATACGATATATAATGACGGGATATAGCGCTAAGTATATATATTCATCTACTTATGCGCGCTTCAGATAGCGTTTATACCAGCGTTCGAAGGCGACGGCGGGCATCGGTTTGGCAAACAAAAATCCTTGcc                                   <  2:1180341/150‑1 (MQ=255)

                                                                                                                     cTAATTATCATCCAGGAATACGATATATAATGACGGGATATAGCGCTAAGTATATATATTCATCTACTTATGCGCGCTTCAGATAGCGTTTATACCAGCGTTCGAAGGCGACGGCGGGCATCGGTTTGGCAAACAAAAATCCTTGCCGCt                                >  2:1321560/1‑150 (MQ=255)

                                                                                                                      tAATTATCATCCAGGAATACGATATATAATGACGGGATATAGCGCTAAGTATATATATTCATCTACTTATGCGCGCTTCAGATAGCGTTTATACCAGCGTTCGAAGGCGACGGCGGGCATCGGTTTGGCAAACAAAAATCCTTGCCGCTc                               <  1:4564113/150‑1 (MQ=255)

                                                                                                                      tAATTATCATCCAGGAATACGATATATAATAACGGGATATAGCGCTAAGTATATATATTCATCTACTTATGCGCGCTTCAGATAGCGTTTATACCAGCGTTCGAAGGCGACGGCGGGCATCGGTTTGGCAAACAAAAATCCTTGCCGCTc                               >  1:3793807/1‑150 (MQ=255)

                                                                                                                       aaTTATCATCCATGAATACGATATATAATGACGGGATATAGCGCTAAGTATATATATTCATCTACTTATGCGCGCTTCAGATAGCGTTTATACCAGCGTTCGAAGGCGACGGCGGGCATCGGTTTGGCAAACAAAAATCCTTGCCGCTCa                              <  2:2634950/150‑1 (MQ=255)

                                                                                                                         ttATCATCCAGGAATACGATATATAATGACGGGATATAGCGCTAAGTATATATATTCATCTACTTATGCGCGCTTCAGATAGCGTTTATACCAGCGTTCGAAGGCGACGGCGGGCATCGGTTTGGCAAACAAAAATCCTTGCCGCTCAtt                            >  1:200957/1‑150 (MQ=255)

                                                                                                                               tcCAGGAATACGATATATAATAACGGGATATAGCGCTAAGTATATATATTCATCTACTTATGCGCGCTTCAGATAGCGTTTATACCAGCGTTCGAAGGCGACGGCGGGCATCGGTTTGGCAAACAAAAATCCTTGCCGCTCATTGATccc                      >  1:2048690/1‑150 (MQ=255)

                                                                                                                               tcCAGGAATACGATATATAATAACGGGATATAGCGCTAAGTATATATATTCATCTACTTATGAGCGCTTCAGAAATCGTTTAAACCAGCGTTCGAAGTCGACGGCGGGCATCGGTTTGGCAAACAAAAATCCTTGCAGCTCATTGATccc                      >  1:1706400/1‑150 (MQ=255)

                                                                                                                                 cAGGAATACGATATATAATGACGGGATATAGCGCTAAGTATATATATTCATCTACTTATGCGCGCTTCAGATAGCGTTTATACCAGCGTTCGAAGGCGACGGCGGGCATCGGTTTGGCAAACAAAAATCCTTGCCGCTCATTGATCCCGt                    >  2:1929068/1‑150 (MQ=255)

                                                                                                                                 cAGGAATACGATATATAATAACGGGATATAGCGCTAAGTATATATCTTCATCTACTTATGCGCGCTTCAGATAGCGTTTATACCAGCGTTCGAAGGCGACGGCGGGCCTCGGTTTGGCAAACAAAAATCCTTGCCGCTCATTGATCCCGt                    >  2:655117/1‑150 (MQ=255)

                                                                                                                                  aGGAATACGATATATAATGACGGGATATAGCGCTAAGTATATATATTCATCTACTTATGCGCGCTTCAGATAGCGTTTATACCAGCGTTCGAAGGCGACGGCGGGCATCGGTTTGGCAAACAAAAATCCTTGCCGCTCATTGATCCCGtt                   <  2:2566474/150‑1 (MQ=255)

                                                                                                                                     aaTACGATATATAATGACGGGATATAGCGCTAAGTATATATATTCATCTACTTATGCGCGCTTCAGATAGCGTTTATACCAGCGTTCGAAGGCGACGGCGGGCATCGGTTTGGCAAACAAAAATCCTTGCCGCTCATTGATCCCGTTCtt                <  2:4788227/150‑1 (MQ=255)

                                                                                                                                      aTACGATATATAATGACGGGATATAGCGCTAAGTATATATATTCATCTACTTATGCGCGCTTCAGATAGCGTTTATACCAGCGTTCGAAGGCGACGGCGGGCATCGGTTTGGCAAACAAAAATCCTTGCCGCTCATTGATCCCGTTCTTg               <  1:122801/150‑1 (MQ=255)

                                                                                                                                       tACGATATATAATGACGGGATATAGCGCTAAGTATATATATTCATCTACTTATGCGCGCTTCAGATAGCGTTTATACCAGCGTTCGAAGGCGACGGCGGGCATCGGTTTGGCAAACAAAAATCCTTGCCGCTCATTGATCCCGTTCTTgg              <  1:2166035/150‑1 (MQ=255)

                                                                                                                                         cGATATATAATGACGGGATATAGCGCTAAGTATATATATTCATCTACTTATGCGCGCTTCAGATAGCGTTTATACCAGCGTTCGAAGGCGACGGCGGGCATCGGTTTGGCAAACAAAAATCCTTGCCGCTCATTGATCCCGTTCTTGGtt            <  2:1734907/150‑1 (MQ=255)

                                                                                                                                          gATATATAATGACGGGATATAGCGCTAAGTATATATATTCATCTACTTATGCGCGCTTCAGATAGCGTTTATACCAGCGTTCGAAGGCGACGGCGGGCATCGGTTTGGCAAACAAAAATCCTTGCCGCTCATTGATCCCGTTCTTGGTTa           <  1:1604707/150‑1 (MQ=255)

                                                                                                                                          gATATATAATGACGGGATATAGCGCTAAGTATATATATTCATCTACTTATGCGCGCTTCAGATAGCGTTTATACCAGCGTTCGAAGGCGACGGCGGGCATCGGTTTGGCAAACAAAAATCCTTGCCGCTCATTGATCCCGTTCTTGGTTa           <  1:2443009/150‑1 (MQ=255)

                                                                                                                                          gATATATAATGACGGGATATAGCGCTAAGTATATATATTCATCTACTTATGCGCGCTTCAGATAGCGTTTATACCAGCGTTCGAAGGCGACGGCGGGCATCGGTTTGGCAAACAAAAATCCTTGCCGCTCATTGATCCCGTTCTTGGTTa           <  2:2758281/150‑1 (MQ=255)

                                                                                                                                           atatatAATGACGGGATATAGCGCTAAGTATATATATTCATCTACTTATGCGCGCTTCAGAGAGCGTTTATACCAGCGTTCGAAGGCGACGGCGGGCATCGGTTTGGCAAACAAAAATCCTTGCCGCTCATTGATCCCGTTCTTGGTTaa          <  1:2050397/150‑1 (MQ=255)

                                                                                                                                            tatataATAACGGGATATAGCGCTAAGTATATATATTCATCTACTTATGCGCGCTTCAGATAGCGTTTATACCAGCGTTCGAAGGCGACGGCGGGCATCGGTTTGGCAAACAAAAATCCTTGCCGCTCATTGATCCCGTTCTTGGTTaaa         >  2:4716694/1‑150 (MQ=255)

                                                                                                                                             atataATGACGGGATATAGCGCTAAGTATATATATTCATCTACTTATGCGCGCTTCAGATAGCGTTTATACCAGCGTTCGAAGGCGACGGCGGGCATCGGTTTGGCAAACAAAAATCCTTGCCGCTCATTGATCCCGTTCTTGGTTaaaa        >  1:4646892/1‑150 (MQ=255)

                                                                                                                                               ataATGACGGGATATAGCGCTAAGTATATATATTCATCTACTTATGCGCGCTTCAGATAGCGTTTATACCAGCGTTCGAAGGCGACGGCGGGCATCGGTTTGGCAAACAAAAATCCTTGCCGCTCATTGATCCCGTTCTTGGTTaaaaaa      <  1:1041622/150‑1 (MQ=255)

                                                                                                                                                 aaTAACGGGATATAGCGCTAAGTATATATATTCATCTACTTATGCGCGCTTCAGATAGCGTTTATACCAGCGTTCGAAGGCGACGGCGGGCATCGGTTTGGCAAACAAAAATCCTTGCCGCTCATTGATCCCGTTCTTGGTTAAAAAAGc    >  2:4478871/1‑150 (MQ=255)

                                                                                                                                                  aTGACGGGATATAGCGCTAAGTATATATATTCATCTACTTATGCGCGCTTCAGATAGCGTTTATACCAGCGTTCGAAGGCGACGGCGGGCATCGGTTTGGCAAACAAAAATCCTTGCCGCTCATTGATCCCGTTCTTGGTTAAAAAAGCa   <  2:423921/150‑1 (MQ=255)

                                                                                                                                                   tGACGGGATATAGCGCTAAGTATATATATTCATCTACTTATGCGCGCTTCAGATAGCGTTTATACCAGCGTTCGAAGGCGACGGCGGGCATCGGTTTGGCAAACAAAAATCCTTGCCGCTCATTGATCCCGTTCTTGGTTAAAAAAGCAt  >  1:1765401/1‑150 (MQ=255)

TACGTTCGGCAACGCGCTGGGCATCAAATTCAGACATCAAGGACATCCTTATAGCAAAAGGGGGAAAATGAATAATGCAAAAATTGCCGCTAAAAGAGAAGTGTTTAACAGCAACGGCTAATTATCATCCAGGAATACGATATATAATGACGGGATATAGCGCTAAGTATATATATTCATCTACTTATGCGCGCTTCAGATAGCGTTTATACCAGCGTTCGAAGGCGACGGCGGGCATCGGTTTGGCAAACAAAAATCCTTGCCGCTCATTGATCCCGTTCTTGGTTAAAAAAGCAT  >  CP009273/1338830‑1339126

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A