Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F98 I0 R1 799 294.1 9752082 92.2% 8991419 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,324,553 C→A 27.4% A187A (GCG→GCT)  obgE ← GTPase involved in cell partioning and DNA repair

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,324,5530CA27.4% 598.0 / 222.6 283A187A (GCG→GCT) obgEGTPase involved in cell partioning and DNA repair
Reads supporting (aligned to +/- strand):  ref base C (104/101);  new base A (36/42);  total (140/143)
Fisher's exact test for biased strand distribution p-value = 5.09e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01

Only 100 of 286 total aligned reads displayed.

CGTTCCAGGTGCTTCAGGAAGCGAATGCCCAGACCTGCGCCTTCCGCAGCGCCTTCAATCAGTCCTGGAATATCGGCAACAACGAAGCTCTTTTCGTTGTCCATTCGTACCACACCCAGACTTGGCACCAGAGTGGTAAACGGATAATCCGCCACTTTCGGTTTAGCCGCCGATACCGCACGAATAAAGGTCGATTTACCCGCGTTTGGCATCCCCAACATACCGACGTCAGCCAGCAGCATCAGCTCCAGCAGCAGCTCGCGCTTATCGCCCGGCGTGCCGTTGGTTTTCTGCCGCGG  >  CP009273/3324404‑3324702
                                                                                                                                                     |                                                                                                                                                     
cGTTCCAGGTGCTTCAGGAAGCGAATGCCCAGACCTGCGCCTTCCGCAGCGCCTTCAATCAGTCCTGGAATATCGGCAACAACGAAGCTCTTTTCGTTGTCCATTCGTACCACACCCAGACTTGGCACCAGAGTGGTAAACGGATAATcc                                                                                                                                                       >  2:2390499/1‑150 (MQ=255)
      aGGTGCTTCAGGAAGCGAATGCCCAGACCTGCGCCTTCCGCAGCGCCTTCAATCAGTCCTGGAATATCGGCAACAACGAAGCTCTTTTCGTTGTCCATTCGTACCACACCCAGACTTGGCACCAGAGTGGTAAACGGATAAGCCGCCACt                                                                                                                                                 >  2:4225007/1‑150 (MQ=255)
         tGCTTCAGGAAGCGAATGCCCAGACCTGCGCCTTCCGCAGCGCCTTCAATCAGTCCTGGAATATCGGCAACAACGAAGCTCTTTTCGTTGTCCATTCGTACCACACCCAGACTTGGCACCAGAGTGGTAAACGGATAATCCGCCACTTTc                                                                                                                                              <  1:4304087/150‑1 (MQ=255)
             tCAGGAAGCGAATGCCCAGACCTGCGCCTTCCGCAGCGCCTTCAATCAGTCCTGGAATATCGGCAACAACGAAGCTCTTTTCGTTGTCCATTCGTACCACACCCAGACTTGGCACCAGAGTGGTAAACGGATAATCCGCCACTTTCGGtt                                                                                                                                          <  1:4231079/150‑1 (MQ=255)
             tCAGGAAGCGAATGCCCAGACCTGCGCCTTCCGCAGCGCCTTCAATCAGTCCTGGAATATCGGCAACAACGAAGCTCTTTTCGTTGTCCATTCGTACCACACCCAGACTTGGCACCAGAGTGGTAAACGGATAATCCGCCACTTTCGGtt                                                                                                                                          <  2:1072032/150‑1 (MQ=255)
              cAGGAAGCGAATGCCCAGACCTGCGCCTTCCGCAGCGCCTTCAATCAGTCCTGGAATATCGGCAACAACGAAGCTCTTTTCGTTGTCCATTCGTACCACACCCAGACTTGGCACCAGAGTGGTAAACGGATAATCCGCCACTTTCGGttt                                                                                                                                         <  2:173142/150‑1 (MQ=255)
               tggATGCGTATGCCCAGACCTGCGCCTTCCGCAGCGCCTTCAATCAGTCCTGGAATATCGGCATCATCGAAGCTCTTTTGGTTGTCCTTTCGTACCACTCCCAGTCTTGGCACCTGAGTGGTAAACGGATAATCAGCCTCTTTCGGTTTa                                                                                                                                        <  1:1002792/149‑1 (MQ=255)
               aGGAAGCGAATGCCCAGACCTGCGCCTTCCGCATCGCCTTCAATCAGTCCTGGAATATCGGCAACAACGAAGCTCTTTTCGTTGTCCATTCGTACCACACCCAGACTTGGCACCAGAGTGGTAAACGGATAATCAGCCACTTTCGGTTTa                                                                                                                                        <  1:4822370/150‑1 (MQ=255)
               aGGAAGCGAATGCCCAGACCTGCGCCTTCCGCAGCGCCTTCAATCAGTCCTGGAATATCGGCAACAACGAAGCTCTTTTCGTTGTCCATTCGTACCACACCCAGACTTGGCACCAGAGTGGTAAACGGATAATCCGCCACTTTCGGTTTa                                                                                                                                        >  1:3862910/1‑150 (MQ=255)
               aGGAAGCGAATGCCCAGACCTGCGCCTTCCGCAGCGCCTTCAATCAGTCCTGGAATATCGGCAACAACGAAGCTCTTTTCGTTGTCCATTCGTACCACACCCAGACTTGGCACCAGAGTGGTAAACGGATAATCCGCCACTTTCGGTTTa                                                                                                                                        >  2:2724250/1‑150 (MQ=255)
                 gAAGCGAATGCCCAGACCTGCGCCTTCCGCAGCGCCTTCAATCAGTCCTGGAATATCGGCAACAACGAAGCTCTTTTCGTTGTCCATTCGTACCACACCCAGACTTGGCACCAGAGTGGTAAACGGATAATCCGCCACTTTCGGTTTAgc                                                                                                                                      >  2:4512862/1‑150 (MQ=255)
                  aaGCGAATGCCCAGACCTGCGCCTTCCGCAGCGCCTTCAATCAGTCCTGGAATATCGGCAACAACGAAGCTCTTTTCGTTGTCCATTCGTACCACACCCAGACTTGGCACCAGAGTGGTAAACGGATAATCAGCCACTTTCGGTTTAgcc                                                                                                                                     <  2:2137050/150‑1 (MQ=255)
                    gCGAATGCCCAGACCTGCGCCTTCCGCAGCGCCTTCAATCAGTCCTGGAATATCGGCAACAACGAAGCTCTTTTCGTTGTCCATTCGTACCACACCCAGACTTGGCACCAGAGTGGTAAACGGATAATCCGCCACTTTCGGTTTAgccgc                                                                                                                                   <  2:3401506/150‑1 (MQ=255)
                     cGAATGCCCAGACCTGCGCCTTCCGCAGCGCCTTCAATCAGTCCTGGAATATCGGCAACAACGAAGCTCTTTTCGTTGTCCATTCGTACCACACCCAGACTTGGCACCAGAGTGGTAAACGGATAATCAGCCACTTTCGGTTTAgccgcc                                                                                                                                  >  1:502457/1‑150 (MQ=255)
                      gAATGCCCAGACCTGCGCCTTCCGCAGCGCCTTCAATCAGTCCTGGAATATCGGCAACAACGAAGCTCTTTTCGTTGTCCATTCGTACCACACCCAGACTTGGCACCAGAGTGGTAAACGGATAATCCGCCACTTTCGGTTTAGccgccg                                                                                                                                 >  1:2576539/1‑150 (MQ=255)
                      gAATGCCCAGACCTGCGCCTTCCGCAGCGCCTTCAATCAGTCCTGGAATATCGGCAACAACGAAGCTCTTTTCGTTGTCCATTCGTACCACACCCAGACTTGGCACCAGAGTGGTAAACGGATAATCAGCCACTTTCGGTTTAGccgccg                                                                                                                                 >  2:4605090/1‑150 (MQ=255)
                       aaTGCCCAGACCTGCGCCTTCCGCAGCGCCTTCAATCAGTCCTGGAATATCGGCAACAACGAAGCTCTTTTCGTTGTCCATTCGTACCACACCCAGACTTGGCACCAGAGTGGTAAACGGATAATCAGCCACTTTCGGTTTAGCCGCCGa                                                                                                                                >  1:3500862/1‑150 (MQ=255)
                        aTGCCCAGACCTGCGCCTTCCGCAGCGCCTTCAATCAGTCCTGGAATATCGGCAACAACGAAGCTCTTTTCGTTGTCCATTCGTACCACACCCAGACTTGGCACCAGAGTGGTAAACGGATAATCCGCCACTTTCGGTTTAGCCGCCGAt                                                                                                                               >  2:2243020/1‑150 (MQ=255)
                           cccAGACCTGCGCCTTCCGCAGCGCCTTCAATCAGTCCTGGAATATCGGCAACAACGAAGCTCTTTTCGTTGTCCATTCGTACCACACCCAGACTTGGCACCAGAGTGGTAAACGGATAATCCGCCACTTTCGGTTTAGCCGCCGATAcc                                                                                                                            <  1:2020968/150‑1 (MQ=255)
                           cccAGACCTGCGCCTTCCGCAGCGCCTTCAATCAGTCCTGGAATATCGGCAACAACGAAGCTCTTTTCGTTGTCCATTCGTACCACACCCAGACTTGGCACCAGAGTGGTAAACGGATAATCAGCCACTTTCGGTTTAGCCGCCGATAcc                                                                                                                            >  2:1659615/1‑150 (MQ=255)
                               gACCTGCGCCTTCCGCAGCGCCTTCAATCAGTCCTGGAATATCGGCAACAACGAAGCTCTTTTCGTTGTCCATTCGTACCACACCCAGACTTGGCACCAGAGTGGTAAACGGATAATCAGCCACTTTCGGTTTAGCCGCCGATACCGCAc                                                                                                                        <  1:2534666/150‑1 (MQ=255)
                                   tGCGCCTTCCGCAGCGCCTTCAATCAGTCCTGGAATATCGGCAACAACGAAGCTCTTTTCGTTGTCCATTCGTACCACACCCAGACTTGGCACCAGAGTGGTAAACGGATAATCCGCCACTTTCGGTTTAGCCGCCGATACCGCACGAAt                                                                                                                    <  1:4328173/150‑1 (MQ=255)
                                    gcgcCTTCCGCAGCGCCTTCAATCAGTCCTGGAATATCGGCAACAACGAAGCTCTTTTCGTTGTCCATTCGTACCACACCCAGACTTGGCACCAGAGTGGTAAACGGATAATCAGCCACTTTCGGTTTAGCCGCCGATACCGCACGAATa                                                                                                                   <  2:3084818/150‑1 (MQ=255)
                                       ccTTCCGCAGCGCCTTCAATCAGTCCTGGAATATCGGCAACAACGAAGCTCTTTTCGTTGTCCATTCGTACCACACCCAGACTTGGCACCAGAGTGGTAAACGGATAATCCGCCACTTTCGGTTTAGCCGCCGATACCGCACGAATAAAg                                                                                                                <  1:237722/150‑1 (MQ=255)
                                        cTTCCGCAGCGCCTTCAATCAGTCCTGGAATATCGGCAACAACGAAGCTCTTTTCGTTGTCCATTCGTACCACACCCAGACTTGGCACCAGAGTGGTAAACGGATAATCCGCCACTTTCGGTTTAGCCGCCGATACCGCACGAATAAAgg                                                                                                               >  1:4046393/1‑150 (MQ=255)
                                        cTTCCGCAGCGCCTTCAATCAGTCCTGGAATATCGGCAACAACGAAGCTCTTTTCGTTGTCCATTCGTACCACACCCAGACTTGGCACCAGAGTGGTAAACGGATAATCCGCCACTTTCGGTTTAGCCGCCGATACCGCACGAATAAAgg                                                                                                               <  1:803345/150‑1 (MQ=255)
                                           ccGCAGCGCCTTCAATCAGTCCTGGAATATCGGCAACAACGAAGCTCTTTTCGTTGTCCATTCGTACCACACCCAGACTTGGCACCAGAGTGGTAAACGGATAATCCGCCACTTTCGGTTTAGCCGCCGATACCGCACGAATAAAGGTCg                                                                                                            <  1:4495721/150‑1 (MQ=255)
                                           ccGCAGCGCCTTCAATCAGTCCTGGAATATCGGCAACAACGAAGCTCTTTTCGTTGTCCATTCGTACCACACCCAGACTTGGCACCAGAGTGGTAAACGGATAATCAGCCACTTTCGGTTTAGCCGCCGATACCGCACGAATAAAGGTCg                                                                                                            <  2:1514361/150‑1 (MQ=255)
                                            cGCAGCGCCTTCAATCAGTCCTGGAATATCGGCAACAACGAAGCTCTTTTCGTTGTCCATTCGTACCACACCCAGACTTGGCACCAGAGTGGTAAACGGATAATCCGCCACTTTCGGTTTAGCCGCCGATACCGCACGAATAAAGGTCGa                                                                                                           >  2:3299047/1‑150 (MQ=255)
                                              cAGCGCCTTCAATCAGTCCTGGAATATCGGCAACAACGAAGCTCTTTTCGTTGTCCATTCGTACCACACCCAGACTTGGCACCAGAGTGGTAAACGGATAATCCGCCACTTTCGGTTTAGCCGCCGATACCGCACGAATAAAGGTCGAtt                                                                                                         <  2:2317346/150‑1 (MQ=255)
                                              cAGCGCCTTCAATCAGTCCTGGAATATCGGCAACAACGAAGCTCTTTTCGTTGTCCATTCGTACCACACCCAGACTTGGCACCAGAGTGGTAAACGGATAATCCGCCACTTTCGGTTTAGCCGCCGATACCGCACGAATAAAGGTCGAtt                                                                                                         <  2:3390958/150‑1 (MQ=255)
                                               aGCGCCTTCAATCAGTCCTGGAATATCGGCAACAACGAAGCTCTTTTCGTTGTCCATTCGTACCACACCCAGACTTGGCACCAGAGTGGTAAACGGATAATCCGCCACTTTCGGTTTAGCCGCCGATACCGCACGAATAAAGGTCGAttt                                                                                                        >  2:2767196/1‑150 (MQ=255)
                                               aGCGCCTTCAATCAGTCCTGGAATATCGGCAACAACGAAGCTCTTTTCGTTGTCCATTCGTACCACACCCAGACTTGGCACCAGAGTGGTAAACGGATAATCCGCCACTTTCGGTTTAGCCGCCGATACCGCACGAATAAAGGTCGAttt                                                                                                        <  1:3811820/150‑1 (MQ=255)
                                                 cgcCTTCAATCAGTCCTGGAATATCGGCAACAACGAAGCTCTTTTCGTTGTCCATTCGTACCACACCCAGACTTGGCACCAGAGTGGTAAACGGATAATCCGCCACTTTCGGTTTAGCCGCCGATACCGCACGAATAAAGGTCGATTTAc                                                                                                      <  1:3323659/150‑1 (MQ=255)
                                                  gcCTTCAATCAGTCCTGGAATATCGGCAACAACGAAGCTCTTTTCGTTGTCCATTCGTACCACACCCAGACTTGGCACCAGAGTGGTAAACGGATAATCAGCCACTTTCGGTTTAGCCGCCGATACCGCACGAATAAAGGTCGATTTAcc                                                                                                     >  1:3202652/1‑150 (MQ=255)
                                                        aaTCAGTCCTGGAATATCGGCAACAACGAAGCTCTTTTCGTTGTCCATTCGTACCACACCCAGACTTGGCACCAGAGTGGTAAACGGATAATCCGCCACTTTCGGTTTAGCCGCCGATACCGCACGAATAAAGGTCGATTTACCCGCGtt                                                                                               >  2:3207703/1‑150 (MQ=255)
                                                         aTCAGTCCTGGAATATCGGCAACAACGAAGCTCTTTTCGTTGTCCATTCGTACCACACCCAGACTTGGCACCAGAGTGGTAAACGGATAATCCGCCACTTTCGGTTTAGCCGCCGATACCGCACGAATAAAGGTCGATTTACCCGCGttt                                                                                              <  1:3459901/150‑1 (MQ=255)
                                                          tCAGTCCTGGAATATCGGCAACAACGAAGCTCTTTTCGTTGTCCATTCGTACCACACCCAGACTTGGCACCAGAGTGGTAAACGGATAATCCGCCACTTTCGGTTTAGCCGCCGATACCGCACGAATAAAGGTCGATTTACCCGCGTTTg                                                                                             >  2:4691813/1‑150 (MQ=255)
                                                          tCAGTCCTGGAATATCGGCAACAACGAAGCTCTTTTCGTTGTCCATTCGTACCACACCCAGACTTGGCACCAGAGTGGTAAACGGATAATCCGCCACTTTCGGTTTAGCCGCCGATACCGCACGAATAAAGGTCGATTTACCCGCGTTTg                                                                                             <  1:1978136/150‑1 (MQ=255)
                                                          tCAGTCCTGGAATATCGGCAACAACGAAGCTCTTTTCGTTGTCCATTCGTACCACACCCAGACTTGGCACCAGAGTGGTAAACGGATAATCAGCCACTTTCGGTTTAGCCGCCGATACCGCACGAATAAAGGTCGATTTACCCGCGTTTg                                                                                             <  1:407954/150‑1 (MQ=255)
                                                          tCAGTCCTGGAATATCGGCAACAACGAAGCTCTTTTCGTTGTCCATTCGTACCACACCCAGACTTGGCACCAGAGTGGTAAACGGATAATCAGCCACTTTCGGTTTAGCCGCCGATACCGCACGAATAAAGGTCGATTTACCCGCGTTTg                                                                                             <  2:793042/150‑1 (MQ=255)
                                                           cAGTCCTGGAATATCGGCAACAACGAAGCTCTTTTCGTTGTCCATTCGTACCACACCCAGACTTGGCACCAGAGTGGTAAACGGATAATCCGCCACTTTCGGTTTAGCCGCCGATACCGCACGAATAAAGGTCGATTTACCCGCGTTTgg                                                                                            <  1:1413904/150‑1 (MQ=255)
                                                           cAGTCCTGGAATATCGGCAACAACGAAGCTCTTTTCGTTGTCCATTCGTACCACACCCAGACTTGGCACCAGAGTGGTAAACGGATAATCAGCCACTTTCGGTTTAGCCGCCGATACCGCACGAATAAAGGTCGATTTACCCGCGTTTgg                                                                                            >  1:148022/1‑150 (MQ=255)
                                                           cAGTCCTGGAATATCGGCAACAACGAAGCTCTTTTCGTTGTCCATTCGTACCACACCCAGACTTGGCACCAGAGTGGTAAACGGATAATCAGCCACTTTCGGTTTAGCCGCCGATACCGCACGAATAAAGGTCGATTTACCCGCGTTTgg                                                                                            >  1:148954/1‑150 (MQ=255)
                                                                  ggAATATCGTCAACAACGAAGCTCTTTTCGTTGTCCATTCGTACCACACCCAGACTTGGCACCAGAGTGGTAAACGGATAATCCGCCACTTTCGGTTTAGCCGCCGATACCGCACGAATAAAGGTCGATTTACCCGCGTTTGGCATcccc                                                                                     <  1:3621135/150‑1 (MQ=255)
                                                                  ggAATATCGGCAACAACGAAGCTCTTTTCGTTGTCCATTCGTACCACACCCAGACTTGGCACCAGAGTGGTAAACGGATAATCCGCCACTTTCGGTTTAGCCGCCGATACCGCACGAATAAAGGTCGATTTACCCGCGTTTGGCATcccc                                                                                     <  1:2784389/150‑1 (MQ=255)
                                                                    aaTATCGGCAACAACGAAGCTCTTTTCGTTGTCCATTCGTACCACACCCAGACTTGGCACCAGAGTGGTAAACGGATAATCAGCCACTTTCGGTTTAGCCGCCGATACCGCACGAATAAAGGTCGATTTACCCGCGTTTGGCATCCCCaa                                                                                   <  2:2688430/150‑1 (MQ=255)
                                                                     atatCGGCAACAACGAAGCTCTTTTCGTTGTCCATTCGTACCACACCCAGACTTGGCACCAGAGTGGTAAACGGATAATCCGCCACTTTCGGTTTAGCCGCCGATACCGCACGAATAAAGGTCGATTTACCCGCGTTTGGCATCCCCAAc                                                                                  <  2:3617661/150‑1 (MQ=255)
                                                                       atCGGCAACAACGAAGCTCTTTTCGTTGTCCATTCGTACCACACCCAGACTTGGCACCAGAGTGGTAAACGGATAATCAGCCACTTTCGGTTTAGCCGCCGATACCGCACGAATAAAGGTCGATTTACCCGCGTTTGGCATCCCCAACAt                                                                                <  1:3750989/150‑1 (MQ=255)
                                                                         cGGCAACAACGAAGCTCTTTTCGTTGTCCATTCGTACCACACCCAGACTTGGCACCAGAGTGGTAAACGGATAATCCGCCACTTTCGGTTTAGCCGCCGATACCGCACGAATAAAGGTCGATTTACCCGCGTTTGGCATCCCCAACATAc                                                                              >  1:630479/1‑150 (MQ=255)
                                                                             aacaacGAAGCTCTTTTCGTTGTCCATTCGTACCACACCCAGACTTGGCACCAGAGTGGTAAACGGATAATCCGCCACTTTCGGTTTAGCCGCCGATACCGCACGAATAAAGGTCGATTTACCCGCGTTTGGCATCCCCAACATACCGAc                                                                          >  2:3113944/1‑150 (MQ=255)
                                                                             aacaacGAAGCTCTTTTCGTTGTCCATTCGTACCACACCCAGACTTGGCACCAGAGTGGTAAACGGATAATCCGCCACTTTCGGTTTAGCCGCCGATACCGCACGAATAAAGGTCGATTTACCCGCGTTTGGCATCCCCAACATACCGAc                                                                          >  2:3159877/1‑150 (MQ=255)
                                                                             aacaacGAAGCTCTTTTCGTTGTCCATTCGTACCACACCCAGACTTGGCACCAGAGTGGTAAACGGATAATCCGCCACTTTCGGTTTAGCCGCCGATACCGCACGAATAAAGGTCGATTTACCCGCGTTTGGCATCCCCAACATACCGAc                                                                          >  2:3791509/1‑150 (MQ=255)
                                                                                aacGAAGCTCTTTTCGTTGTCCATTCGTACCACACCCAGACTTGGCACCAGAGTGGTAAACGGATAATCCGCCACTTTCGGTTTAGCCGCCGATACCGCACGAATAAAGGTCGATTTACCCGCGTTTGGCATCCCCAACATACCGACGTc                                                                       >  2:90467/1‑150 (MQ=255)
                                                                                aacGAAGCTCTTTTCGTTGTCCATTCGTACCACACCCAGACTTGGCACCAGAGTGGTAAACGGATAATCAGCCACTTTCGGTTTAGCCGCCGATACCGCACGAATAAAGGTCGATTTACCCGCGTTTGGCATCCCCAACATACCGACGTc                                                                       >  1:563484/1‑150 (MQ=255)
                                                                                aacGAAGCTCTTTTCGTTGTCCATTCGTACCACACCCAGACTTGGCACCAGAGTGGTAAACGGATAATCAGCCACTTTCGGTTTAGCCGCCGATACCGCACGAATAAAGGTCGATTTACCCGCGTTTGGCATCCCCAACATACCGACGTc                                                                       >  1:2408263/1‑150 (MQ=255)
                                                                                   gAAGCTCTTTTCGTTGTCCATTCGTACCACACCCAGACTTGGCACCAGAGTGGTAAACGGATAATCCGCCACTTTCGGTTTAGCCGCCGATACCGCACGAATAAAGGTCGATTTACCCGCGTTTGGCATCCCCAACATACCGACGTcagc                                                                    >  2:1546864/1‑150 (MQ=255)
                                                                                   gAAGCTCTTTTCGTTGTCCATTCGTACCACACCCAGACTTGGCACCAGAGTGGTAAACGGATAATCCGCCACTTTCGGATTAGCCGCCGATACCGCACGAATAAAGGTCGATTTACCCGCGTTAGGCATCCCCAACATACCGACGTcagc                                                                    >  2:2886915/1‑150 (MQ=255)
                                                                                   gAAGCTCTTTTCGTTGTCCATTCGTACCACACCCAGACTTGGCACCAGAGTGGTAAACGGATAATCAGCCACTTTCGGTTTAGCCGCCGATACCGCACGAATAAAGGTCGATTTACCCGCGTTTGGCATCCCCAACATACCGACGTcagc                                                                    >  1:3529325/1‑150 (MQ=255)
                                                                                    aaGCTCTTTTCGTTGTCCATTCGTACCACACCCAGACTTGGCACCAGAGTGGTAAACGGATAATCAGCCACTTTCGGTTTAGCCGCCGATACCGCACGAATAAAGGTCGATTTACCCGCGTTTGGCATCCCCAACATACCGACGTcagcc                                                                   <  2:2621560/150‑1 (MQ=255)
                                                                                     aGCTCTTTTCGTTGTCCATTCGTACCACACCCAGACTTGGCACCAGAGTGGTAAACGGATAATCCGCCACTTTCGGTTTAGCCGCCGATACCGCACGAATAAAGGTCGATTTACCCGCGTTTGGCATCCCCAACATACCGACGTcagcca                                                                  <  1:2699706/150‑1 (MQ=255)
                                                                                      gCTCTTTTCGTTGTCCATTCGTACCACACCCAGACTTGGCACCAGAGTGGTAAACGGATAATCCGCCACTTTCGGTTTAGCCGCCGATACCGCACGAATAAAGGTCGATTTACCCGCGTTTGGCATCCCCAACATACCGACGTcagccag                                                                 <  2:1126915/150‑1 (MQ=255)
                                                                                          ttttCGTTGTCCATTCGTACCACACCCAGACTTGGCACCAGAGTGGTAAACGGATAATCAGCCACTTTCGGTTTAGCCGCCGATACCGCACGAATAAAGGTCGATTTACCCGCGTTTGGCATCCCCAACATACCGACGTCAGCCagcagc                                                             <  1:1576963/150‑1 (MQ=255)
                                                                                           tttCGTTGTCCATTCGTACCACACCCAGACTTGGCACCAGAGTGGTAAACGGATAATCCGCCACTTTCGGTTTAGCCGCCGATACCGCACGAATAAAGGTCGATTTACCCGCGTTTGGCATCCCCAACATACCGACGTCAGCCAgcagca                                                            <  2:4374840/150‑1 (MQ=255)
                                                                                             tCGTTGTCCATTCGTACCACACCCAGACTTGGCACCAGAGTGGTAAACGGATAATCAGCCACTTTCGGTTTAGCCGCCGATACCGCACGAATAAAGGTCGATTTACCCGCGTTTGGCATCCCCAACATACCGACGTCAGCCAGCAGCATc                                                          <  2:4639083/150‑1 (MQ=255)
                                                                                              cGTTGTCCATTCGTACCACACCCAGACTTGGCACCAGAGTGGTAAACGGATAATCCGCCACTTTCGGTTTAGCCGCCGATACCGCACGAATAAAGGTCGATTTACCCGCGTTTGGCATCCCCAACATACCGACGTCAGCCAGCAGCATCa                                                         <  1:2151403/150‑1 (MQ=255)
                                                                                               gTTGTCCATTCGTACCACACCCAGACTTGGCACCAGAGTGGTAAACGGATAATCAGCCACTTTCGGTTTAGCCGCCGATACCGCACGAATAAAGGTCGATTTACCCGCGTTTGGCATCCCCAACATACCGACGTCAGCCAGCAGCATCAg                                                        <  2:2169272/150‑1 (MQ=255)
                                                                                                 tGTCCATTCGTACCACACCCAGACTTGGCACCAGAGTGGTAAACGGATAATCCGCCACTTTCGGTTTAGCCGCCGATACCGCACGAATAAAGGTCGATTTACCCGCGTTTGGCATCCCCAACATACCGACGTCAGCCAGCAGCATCAGCt                                                      >  1:2961110/1‑150 (MQ=255)
                                                                                                  gTCCATTCGTACCACACCCAGACTTGGCACCAGAGTGGTAAACGGATAATCCGCCACTTTCGGTTTAGCCGCCGATACCGCACGAATAAAGGTCGATTTACCCGCGTTTGGCATCCCCAACATACCGACGTCAGCCAGCAGCATCAGCTc                                                     >  1:1062110/1‑150 (MQ=255)
                                                                                                     cATTCGTACCACACCCAGACTTGGCACCAGAGTGGTAAACGGATAATCCGCCACTTTCGGTTTAGCCGCCGATACCGCACGAATAAAGGTCGATTTACCCGCGTTTGGCATCCCCAACATACCGACGTCAGCCAGCAGCATCAGCTCcag                                                  >  2:4755485/1‑150 (MQ=255)
                                                                                                          gTACCACACCCAGACTTGGCACCAGAGTGGTAAACGGATAATCCGCCACTTTCGGTTTAGCCGCCGATACCGCACGAATAAAGGTCGATTTACCCGCGTTTGGCATCCCCAACATACCGACGTCAGCCAGCAGCATCAGCTCcagcagca                                             <  1:2457016/150‑1 (MQ=255)
                                                                                                           tACCACACCCAGACTTGGCACCAGAGTGGTAAACGGATAATCCGCCACTTTCGGTTTAGCCGCCGATACCGCACGAATAAAGGTCGATTTACCCGCGTTTGGCATCCCCAACATACCGACGTCAGCCAGCAGCATCAGCTCcagcagcag                                            <  2:1209440/150‑1 (MQ=255)
                                                                                                           tACCACACCCAGACTTGGCACCAGAGTGGTAAACGGATAATCCGCCACTTTCGGTTTAGCCGCCGATACCGCACGAATAAAGGTCGATTTACCCGCGTTTGGCATCCCCAACATACCGACGTCAGCCAGCAGCATCAGCTCcagcagcag                                            <  2:2458503/150‑1 (MQ=255)
                                                                                                            aCCACACCCAGACTTGGCACCAGAGTGGTAAACGGATAATCAGCCACTTTCGGTTTAGCCGCCGATACCGCACGAATAAAGGTCGATTTACCCGCGTTTGGCATCCCCAACATACCGACGTCAGCCAGCAGCATCAGCTCCagcagcagc                                           >  2:1808761/1‑150 (MQ=255)
                                                                                                              cacaCCCAGACTTGGCACCAGAGTGGTAAACGGATAATCAGCCACTTTCGGTTTAGCCGCCGATACCGCACGAATAAAGGTCGATTTACCCGCGTTTGGCATCCCCAACATACCGACGTCAGCCAGCAGCATCAGCTCCAGCAGCAGCTc                                         <  1:39197/150‑1 (MQ=255)
                                                                                                                 acCCAGACTTGGCACCAGAGTGGTAAACGGATAATCCGCCACTTTCGGTTTAGCCGCCGATACCGCACGAATAAAGGTCGATTTACCCGCGTTTGGCATCCCCAACATACCGACGTCAGCCAGCAGCATCAGCTCCAGCAGCAGCTcgcg                                      <  1:1711396/150‑1 (MQ=255)
                                                                                                                   ccAGACTTGGCACCAGAGTGGTAAACGGATAATCCGCCACTTTCGGTTTAGCCGCCGATACCGCACGAATAAAGGTCGATTTACCCGCGTTTGGCATCCCCAACATACCGACGTCAGCCAGCAGCATCAGCTCCAGCAGCAGCTCGCGCt                                    <  2:2646637/150‑1 (MQ=255)
                                                                                                                   ccAGACTTGGCACCAGAGTGGTAAACGGATAATCCGCCACTTTCGGTTTAGCCGCCGATACCGCACGAATAAAGGTCGATTTACCCGCGTTTGGCATCCCCAACATACCGACGTCAGCCAGCAGCATCAGCTCCAGCAGCAGCTCGCGCt                                    <  1:4423852/150‑1 (MQ=255)
                                                                                                                          tGGCACCAGAGTGGTAAACGGATAATCAGCCACTTTCTGTTTAGCCGCCGATACCGCACGAATAAAGGTCGATTTACCCGCGTTTGGCATCCCCTACATACCGACGTCAGCCAGCAGCATCAGCTCCAGCAGCAGCTCGCGCTTATCGcc                             <  1:1877963/150‑1 (MQ=255)
                                                                                                                            gCACCAGAGTGGTAAACGGATAATCCGCCACTTTCGGTTTAGCCGCCGATACCGCACGAATAAAGGTCGATTTACCCGCGTTTGGCATCCCCAACATACCGACGTCAGCCAGCAGCATCAGCTCCAGCAGCAGCTCGCGCTTATCGCCCg                           >  1:2347951/1‑150 (MQ=255)
                                                                                                                             cACCAGAGTGGTAAACGGATAATCCGCCACTTTCGGTTTAGCCGCCGATACCGCACGAATAAAGGTCGATTTACCCGCGTTTGGCATCCCCAACATACCGACGTCAGCCAGCAGCATCAGCTCCAGCAGCAGCTCGCGCTTATCGCCCgg                          >  2:653603/1‑150 (MQ=255)
                                                                                                                               ccAGAGTGGTAAACGGATAATCCGCCACTTTCGGTTTAGCCGCCGATACCGCACGAATAAAGGTCGATTTACCCGCGTTTGGCATCCCCAACATACCGACGTCAGCCAGCAGCATCAGCTCCAGCAGCAGCTCGCGCTTATCGCCCGGCg                        <  1:2804860/150‑1 (MQ=255)
                                                                                                                                cAGAGTGGTAAACTGATAATCCGCCACTTTCGGTTTAGCCGCCGATACCGCACGAATAAAGGTCGATTTACCCGCGTTTGGCATCCCCAACATACCGACGTCAGCCAGCAGCATCAGCTCCAGCAGCAGCTCGCGCTTATCGCCCGGCGt                       <  1:2243020/150‑1 (MQ=255)
                                                                                                                                cAGAGTGGTAAACGGATAATCCGCCACTTTCGGTTTAGCCGCCGATACCGCACGAATAAAGGTCGATTTACCCGCGTTTGGCATCCCCAACATACCGACGTCAGCCAGCAGCATCAGCTCCAGCAGCAGCTCGCGCTTATCGCCCGGCGt                       >  1:4478261/1‑150 (MQ=255)
                                                                                                                                cAGAGTGGTAAACGGATAATCCGCCACTTTCGGTTTAGCCGCCGATACCGCACGAATAAAGGTCGATTTACCCGCGTTTGGCATCCCCAACATACCGACGTCAGCCAGCAGCATCAGCTCCAGCAGCAGCTCGCGCTTATCGCCCGGCGt                       <  2:2961110/150‑1 (MQ=255)
                                                                                                                                   agTGGTAAACGGATAATCCGCCACTTTCGGTTTAGCCGCCGATACCGCACGAATAAAGGTCGATTTACCCGCGTTTGGCATCCCCAACATACCGACGTCAGCCAGCAGCATCAGCTCCAGCAGCAGCTCGCGCTTATCGCCCGGCGTGcc                    <  2:514892/150‑1 (MQ=255)
                                                                                                                                       gTAAACGGATAATCCGCCACTTTCGGTTTAGCCGCCGATACCGCACGAATAAAGGTCGATTTACCCGCGTTTGGCATCCCCAACATACCGACGTCAGCCAGCAGCATCAGCTCCAGCAGCAGCTCGCGCTTATCGCCCGGCGTGCCGTTg                <  1:1609659/150‑1 (MQ=255)
                                                                                                                                        tAAACGGATAATCCGCCACTTTCGGTTTAGCCGCCGATACCGCACGAATAAAGGTCGATTTACCCGCGTTTGGCATCCCCAACATACCGACGTCAGCCAGCAGCATCAGCTCCAGCAGCAGCTCGCGCTTATCGCCCGGCGTGCCGTTgg               >  2:3830965/1‑150 (MQ=255)
                                                                                                                                         aaaCGGATAATCCGCCACTTTCGGTTTAGCCGCCGATACCGCACGAATAAAGGTCGATTTACCCGCGTTTGGCATCCCCAACATACCGACGTCAGCCAGCAGCATCAGCTCCAGCAGCAGCTCGCGCTTATCGCCCGGCGTGCCGTTGGt              <  1:1319874/150‑1 (MQ=255)
                                                                                                                                         aaaCGGATAATCCGCCACTTTCGGTTTAGCCGCCGATACCGCACGAATAAAGGTCGATTTACCCGCGTTTGGCATCCCCAACATACCGACGTCAGCCAGCAGCATCAGCTCCAGCAGCAGCTCGCGCTTATCGCCCGGCGTGCCGTTGGt              >  1:4618439/1‑150 (MQ=255)
                                                                                                                                          aaCGGATAATCCGCCACTTTCGGTTTAGCCGCCGATACCGCACGAATAAAGGTCGATTTACCCGCGTTTGGCATCCCCAACATACCGACGTCAGCCAGCAGCATCAGCTCCAGCAGCAGCTCGCGCTTATCGCCCGGCGTGCCGTTGGtt             >  1:4367555/1‑150 (MQ=255)
                                                                                                                                            cGGATAATCCGCCACTTTCGGTTTAGCCGCCGATACCGCACGAATAAAGGTCGATTTACCCGCGTTTGGCATCCCCAACATACCGACGTCAGCCAGCAGCATCAGCTCCAGCAGCAGCTCGCGCTTATCGCCCGGCGTGCCGTTGGtttt           <  2:2261712/150‑1 (MQ=255)
                                                                                                                                             ggATAATCCGCCACTTTCGGTTTAGCCGCCGATACCGCACGAATAAAGGTCGATTTACCCGCGTTTGGCATCCCCAACATACCGACGTCAGCCAGCAGCATCAGCTCCAGCAGCAGCTCGCGCTTATCGCCCGGCGTGCCGTTGGTTTTc          <  1:3675534/150‑1 (MQ=255)
                                                                                                                                             ggATAATCCGCCACTTTCGGTTTAGCCGCCGATACCGCACGAATAAAGGTCGATTTACCCGCGTTTGGCATCCCCAACATACCGACGTCAGCCAGCAGCATCAGCTCCAGCAGCAGCTCGCGCTTATCGCCCGGCGTGCCGTTGGTTTTc          >  2:4048427/1‑150 (MQ=255)
                                                                                                                                                tAATCCGCCACTTTCGGTTTAGCCGCCGATACCGCACGAATAAAGGTCGATTTACCCGCGTTTGGCATCCCCAACATACCGACGTCAGCCAGCAGCATCAGCTCCAGCAGCAGCTCGCGCTTATCGCCCGGCGTGCCGTTGGTTTTCTGc       <  2:982632/150‑1 (MQ=255)
                                                                                                                                                tAATCCGCCACTTTCGGTTTAGCCGCCGATACCGCACGAATAAAGGTCGATTTACCCGCGTTTGGCATCCCCAACATACCGACGTCAGCCAGCAGCATCAGCTCCAGCAGCAGCTCGCGCTTATCGCCCGGCGTGCCGTTGGTTTTCTGc       >  1:3401214/1‑150 (MQ=255)
                                                                                                                                                tAATCCGCCACTTTCGGTTTAGCCGCCGATACCGCACGAATAAAGGTCGATTTACCCGCGTTTGGCATCCCCAACATACCGACGTCAGCCAGCAGCATCAGCTCCAGCAGCAGCTCGCGCTTATCGCCCGGCGTGCCGTTGGTTTTCTGc       >  1:1199175/1‑150 (MQ=255)
                                                                                                                                                tAATCCGCCACTTTCGGTTTAGCCGCCGATACCGCACGAATAAAGGTCGATTTACCCGCGTTTGGCATCCCCAACATACCGACGTCAGCCAGCAGCATCAGCTCCAGCAGCAGCTCGCGCTTATCGCCCGGCGTGCCGTTGGTTTTCTGc       >  1:3950406/1‑150 (MQ=255)
                                                                                                                                                 aaTCCGCCACTTTCGGTTTAGCCGCCGATACCGCACGAATAAAGGTCGATTTACCCGCGTTTGGCATCCCCAACATACCGACGTCAGCCAGCAGCATCAGCTCCAGCAGCAGCTCGCGCTTATCGCCCGGCGTGCCGTTGGTTTTCTGcc      <  1:1151835/150‑1 (MQ=255)
                                                                                                                                                  aTCCGCCACTTTCGGTTTAGCCGCCGATACCGCACGAATAAAGGTCGATTTACCCGCGTTTGGCATCCCCAACATACCGACGTCAGCCAGCAGCATCAGCTCCAGCAGCAGCTCGCGCTTATCGCCCGGCGTGCCGTTGGTTTTCTGCcg     >  2:3471228/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
CGTTCCAGGTGCTTCAGGAAGCGAATGCCCAGACCTGCGCCTTCCGCAGCGCCTTCAATCAGTCCTGGAATATCGGCAACAACGAAGCTCTTTTCGTTGTCCATTCGTACCACACCCAGACTTGGCACCAGAGTGGTAAACGGATAATCCGCCACTTTCGGTTTAGCCGCCGATACCGCACGAATAAAGGTCGATTTACCCGCGTTTGGCATCCCCAACATACCGACGTCAGCCAGCAGCATCAGCTCCAGCAGCAGCTCGCGCTTATCGCCCGGCGTGCCGTTGGTTTTCTGCCGCGG  >  CP009273/3324404‑3324702

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A