Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F16 I0 R1 235 70.4 2387208 96.6% 2306042 149.2

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 8,732 T→C 70.6% R165R (CGT→CGC)  talB → transaldolase B

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092738,7320TC70.6% 320.7 / 189.7 218R165R (CGT→CGC) talBtransaldolase B
Reads supporting (aligned to +/- strand):  ref base T (32/32);  new base C (91/63);  total (123/95)
Fisher's exact test for biased strand distribution p-value = 2.33e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.48e-06

Only 100 of 220 total aligned reads displayed.

ATCAAACTCTACAACGATGCTGGTATTAGCAACGATCGTATTCTGATCAAACTGGCTTCTACCTGGCAGGGTATCCGTGCTGCAGAACAGCTGGAAAAAGAAGGCATCAACTGTAACCTGACCCTGCTGTTCTCCTTCGCTCAGGCTCGTGCTTGTGCGGAAGCGGGCGTGTTCCTGATCTCGCCGTTTGTTGGCCGTATTCTTGACTGGTACAAAGCGAATACCGATAAGAAAGAGTACGCTCCGGCAGAAGATCCGGGCGTGGTTTCTGTATCTGAAATCTACCAGTACTACAAAG  >  CP009273/8583‑8880
                                                                                                                                                     |                                                                                                                                                    
aTCAAACTCTACAACGATGCTGGTATTAGCAACGATCGTATTCTGATCAAACTGGCTTCTACCTGGCAGGGTATCCGTGCTGCAGAACAGCTGGAAAAAGAAGGCATCAACTGTAACCTGACCCTGCTGTTCTCCTTCGCTCAGGCTCGt                                                                                                                                                      >  2:535739/1‑150 (MQ=255)
   aagctgtACAACGACGCGGGCATCAGCAACGATCGCATTCTGATCAAACTGGCCTCCACCTGGCAGGGCATTCGCGCCGCTGAACAGCTGGAAAAAGAAGGCATTAACTGTAACCTGACGCTGCTGTTCTCCTTCGCTCAGGCTCGCGCt                                                                                                                                                   <  1:545735/144‑1 (MQ=255)
      ctgtACAACGACGCGGGCATCAGCAACGATCGCATTCTGATCAAACTGGCCTCCACCTGGCAGGGCATTCGCGCCGCTGAACAGCTGGAAAAAGAAGGCATTAACTGTAACCTGACGCTGCTGTTCTCCTTCGCTCAGGCTCGCGCTtgc                                                                                                                                                >  1:601115/4‑149 (MQ=255)
         tACAACGATGCTGGTATTAGCAACGATCGTATTCTGATCAAACTGGCTTCTACCTGGCAGGGTATCCGTGCTGCAGAACAGCTGGAAAAAGAAGGCATCAACTGTAACCTGACCCTGCTGTTCTCCTTCGCTCAGGCTCGTGCTTGTGCg                                                                                                                                             >  1:1180817/1‑150 (MQ=255)
         tACAACGACGCGGGCATCAGCAACGATCGCATTCTGATCAAACTGGCCTCCACCTGGCAGGGCATTCGCGCCGCTGAACAGCTGGAAAAAGAAGGCATTAACTGTAACCTGACGCTGCTGTTCTCCTTCGCTCAGGCTCGCGCTTgcgcc                                                                                                                                             <  2:726353/150‑2 (MQ=255)
         tACAACGACGCGGGCATCAGCAACGATCGCATTCTGATCAAACTGGCCTCCACCTGGCAGGGCATTCGCGCCGCTGAACAGCTGGAAAAAGAAGGCATTAACTGTAACCTGACGCTGCTGTTCTCCTTCGCTCAGGCTCGCGCTTgcgcc                                                                                                                                             >  1:710760/1‑149 (MQ=255)
          aCAACGACGCGGGCATCAGCAACGATCGCATTCTGATCAAACTGGCCTCCACCTGGCAGGGCATTCGCGCCGCTGAACAGCTGGAAAAAGAAGGCATTAACTGTAACCTGACGCTGCTGTTCTCCTTCGCTCAGGCTCGCGCTTgcgcc                                                                                                                                             >  1:641708/1‑148 (MQ=255)
           cAACGACGCGGGCATCAGCAACGATCGCATTCTGATCAAACTGGCCTCCACCTGGCAGGGCATTCGCGCCGCTGAACAGCTGGAAAAAGAAGGCATTAACTGTAACCTGACGCTGCTGTTCTCCTTCGCTCAGGCTCGCGCTTGCGCCGa                                                                                                                                           <  2:374581/150‑1 (MQ=255)
            aaCGACGCGGGCATCAGCAACGATCGCATTCTGATCAAACTGGCCTCCACCTGGCAGGGCATTCGCGCCGCTGAACAGCTGGAAAAAGAAGGCATTAACTGTAACCTGACGCTGCTGTTCTCCTTCGCTCAGGCTCGCGCTTgcgccg                                                                                                                                            >  1:435941/1‑146 (MQ=255)
             aCGATGCTGGTATTAGCAACGATCGTATTCTGATCAAACTGGCTTCTACCTGGCAGGGTATCCGTGCTGCAGAACAGCTGGAAAAAGAAGGCATCAACTGTAACCTGACCCTGCTGTTCTCCTTCGCTCAGGCTCGTGCTTGTGCGGAAg                                                                                                                                         <  1:657712/150‑1 (MQ=255)
              cGACGCGGGCATCAGCAACGATCGCATTCTGATCAAACTGGCCTCCACCTGGCAGGGCATTCGCGCCGCTGAACAGCTGGAAAAAGAAGGCATTAACTGTAACCTGACGCTGCTGTTCTCCTTCGCTCAGGCTCGCGCTTGCGCCGAAGc                                                                                                                                        <  1:612633/150‑1 (MQ=255)
                     ggTATTAGCAACGATCGTATTCTGATCAAACTGGCTTCTACCTGGCAGGGTATCCGTGCTGCAGAACAGCTGGAAAAAGAAGGCATCAACTGTAACCTGACCCTGCTGTTCTCCTTCGCTCAGGCTCGTGCTTGTGc                                                                                                                                              <  2:448338/137‑1 (MQ=255)
                     ggTATTAGCAACGATCGTATTCTGATCAAACTGGCTTCTACCTGGCAGGGTATCCGTGCTGCAGAACAGCTGGAAAAAGAAGGCATCAACTGTAACCTGACCCTGCTGTTCTCCTTCGCTCAGGCTCGTGCTTGTGCGGAAGCGGGCgtg                                                                                                                                 >  2:139655/1‑150 (MQ=255)
                      gTATTAGCAACGATCGTATTCTGATCAAACTGGCTTCTACCTGGCAGGGTATCCGTGCTGCAGAACAGCTGGAAAAAGAAGGCATCAACTGTAACCTGACCCTGCTGTTCTCCTTCGCTCAGGCTCGTGCTTGTGCGGAAGCGGGCgtgt                                                                                                                                >  2:849238/1‑150 (MQ=255)
                       tATTAGCAACGATCGTATTCTGATCAAACTGGCTTCTACCTGGCAGGGTATCCGTGCTGCAGAACAGCTGGAAAAAGAAGGCATCAACTGTAACCTGACCCTGCTGTTCTCCTTCGCTCAGGCTCGTGCTTGTGCGGAAGCGGGCGTGtt                                                                                                                               >  2:977741/1‑150 (MQ=255)
                        gtcaGCAACGATCGCATTCTGATCAAACTGGCCACCACCTGGCAGGGCATTCGCGCCGCTGAACAGCTGGAAAAAGAAGGCATTAACTGTAACCTGACGCTGCTGTTCTCCTTCGCTCAGGCTCGCGCTTGCGCCGAAGCGGGCGTCTTc                                                                                                                              >  2:358494/4‑150 (MQ=255)
                        accaGCAACGATCGCATTCCGATCAAACTGGCCTCCACCTGGCAGGGCATTCGCGCCGCTGAACAGCTGGAAAAAGCAGGCATTAACTGTAACCTGACGCTGCTGTTCTCCTTCGCTCAGGCTCGCGCTTGCGCCGAAGCGGGCGTCTTc                                                                                                                              >  2:659646/4‑150 (MQ=255)
                           aGCAACGATCGCATTCTGATCAAACTGGCCTCCACCTGGCAGGGCATTCGCGCCGCTGAACAGCTGGAAAAAGAAGGCATTAACTGTAACCTGACGCTGCTGTTCTCCTTCGCTCAGGCTCGCGCTTGCGCCGAAGCGGGCGTCTTCCt                                                                                                                            >  2:861480/1‑149 (MQ=255)
                            gCAACGATCGTATTCTGATCAAACTGGCTTCTACCTGGCAGGGTATCCGTGCTGCAGAACAGCTGGAAAAAGAAGGCATCAACTGTAACCTGACCCTGCTGTTCTCCTTCGCTCAGGCTCGTGCTTGTGCGGAAGCGGGCGTGTTCCTg                                                                                                                           <  1:594738/149‑1 (MQ=255)
                             cAACGATCGCATTCTGATCAAACTGGCCTCCACCTGGCAGGGCATTCGCGCCGCTGAACAGCTGGAAAAAGAAGGCATTAACTGTAACCTGACGCTGCTGTTCTCCTTCGCTCAGGCTCGCGCTTGCGCCGAAGCGGGCGTCtt                                                                                                                               >  1:1096672/1‑144 (MQ=255)
                              aaCGATCGTATTCTGATCAAACTGGCTTCTACCTGGCAGGGTATCCGTGCTGCAGAACAGCTGGAAAAAGAAGGCATCAACTGTAACCTGACCCTGCTGTTCTCCTTCGCTCAGGCTCGTGCTTGTGCGGAAGCGGGCGTGTTCCTGAtc                                                                                                                        >  1:685747/1‑150 (MQ=255)
                              aaCGATCGCATTCTGATCAAACTGGCCTCCACCTGGCAGGGCATTCGCGCCGCTGAACAGCTGGAAAAAGAAGGCATTAACTGTAACCTGACGCTGCTGTTCTCCTTCGCTCAGGCTCGCGCTTGCGCCGAAGCGGGCGTCTTCCTGAtt                                                                                                                        <  1:661181/150‑2 (MQ=255)
                                 gATCGCATTCTGATCAAACTGGCCTCCACCTGGCAGGGCATTCGCGCCGCTGAACAGCTGGAAAAAGAAGGCATTAACTGTAACCTGACGCTGCTGTTCTCCTTCGCTCAGGCTCGCGCTTGCGCCGAAGCGGGCGTCTTCCTGATTTCg                                                                                                                     >  2:215179/1‑150 (MQ=255)
                                  aTCGCATTCTGATCAAACTGGCCTCCACCTGGCAGGGCATTCGCGCCGCTGAACAGCTGGAAAAAGAAGGCATTAACTGTAACCTGACGCTGCTGTTCTCCTTCGCTCAGGCTCGCGCTTGCGCCGAAGCGGGCGTCTTCCTGATTTCGc                                                                                                                    >  2:794425/1‑150 (MQ=255)
                                   tCGCATTCTGATCAAACTGGCCTCCACCTGGCAGGGCATTCGCGCCGCTGAACAGCTGGAAAAAGAAGGCATTAACTGTAACCTGACGCTGCTGTTCTCCTTCGCTCAGGCTCGCGCTTGCGCCGAAGCGGGCGTCTTCCTGATTTCGcc                                                                                                                   <  1:580567/150‑1 (MQ=255)
                                    cgcaTTCTGATCAAACTGGCCTCCACCTGGCAGGGCATTCGCGCCGCTGAACAGCTGGAAAAAGAAGGCATTAACTGTAACCTGACGCTGCTGTTCTCCTTCGCTCAGGCTCGCGCTTGCGCCGAAGCGGGCGTCTTCCTGATTTCGCCg                                                                                                                  >  2:552482/4‑150 (MQ=255)
                                     gcaTTCTGATCAAACTGGCCTCCACCTGGCAGGGCATTCGCGCCGCTGAACAGCTGGAAAAAGAAGGCATTAACTGTAACCTGACGCTGCTGTTCTCCTTCGCTCAGGCTCGCGCTTGCGCCGAAGCGGGCGTCTTCCTGATTTCGCCGt                                                                                                                 <  2:42966/148‑1 (MQ=255)
                                        ttCTGATCAAACTGGCCTCCACCTGGCAGGGCATTCGCGCCGCTGAACAGCTGGAAAAAGAAGGCATTAACTGTAACCTGACGCTGCTGTTCTCCTTCGCTCAGGCTCGCGCTTGCGCCGAAGCGGGCGTCTTCCTGATTTCGCCGttcg                                                                                                              >  2:390912/1‑148 (MQ=255)
                                         tCTGATCAAACTGGCCTCCACCTGGCAGGGCATTCGCGCCGCTGAACAGCTGGAAAAAGAAGGCATTAACTGTAACCTGACGCTGCTGTTCTCCTTCGCTCAGGCTCGCGCTTGCGCCGAAGCGGGCGTCTTCCTGATTTCGCCGTtcgt                                                                                                             >  2:743061/1‑150 (MQ=255)
                                           tGATCAAACTGGCTTCTACCTGGCAGGGTATCCGTGCTGCAGAACAGCTGGAAAAAGAAGGCATCAACTGTAACCTGACCCTGCTGTTCTCCTTCGCTCAGGCTCGTGCTTGTGCGGAAGCGGGCGTGTTCCTGATCTCGCCGTttgttg                                                                                                           >  2:464164/1‑150 (MQ=255)
                                            gATCAAACTGGCCTCCACCTGGCAGGGCATTCGCGCCGCTGAACAGCTGGAAAAAGAAGGCATTAACTGTAACCTGACGCTGCTGTTCTCCTTCGCTCAGGCTCGCGCTTGCGCCGAAGCGGGCGTCTTCCTGATTTCGCCGTTCGTTgg                                                                                                          >  1:458366/1‑150 (MQ=255)
                                             aTCAAACTGGCTTCTACCTGGCAGGGTATCCGTGCTGCAGAACAGCTGGAAAAAGAAGGCATCAACTGTAACCTGACCCTGCTGTTCTCCTTCGCTCAGGCTCGTGCTTGTGCGGAAGCGGGCGTGTTCCTGATCTCGCCGTTTGTTgg                                                                                                          <  1:177087/149‑1 (MQ=255)
                                             aTCAAACTGGCCTCCACCTGGCAGGGCATTCGCGCCGCTGAACAGCTGGAAAAAGAAGGCATTAACTGTAACCTGACGCTGCTGTTCTCCTTCGCTCAGGCTCGCGCTTGCGCCGAAGCGGGCGTCTTCCTGATTTCGCCGTTCGTTGGc                                                                                                         >  1:385261/1‑150 (MQ=255)
                                             aTCAAACTGGCCTCCACCTGGCAGGGCATTCGCGCCGCTGAACAGCTGGAAAAAGAAGGCATTAACTGTAACCTGACGCTGCTGTTCTCCTTCGCACAGGCTCGCGCTTGCGCCGAAGCGGGCGTCTTCCTGATTTCGCcgttcgtt                                                                                                            <  2:1052632/147‑1 (MQ=255)
                                                 aaCTGGCTTCTACCTGGCAGGGTATCCGTGCTGCAGAACAGCTGGAAAAAGAAGGCATCAACTGTAACCTGACCCTGCTGTTCTCCTTCGCTCAGGCTCGTGCTTGTGCGGAAGCGGGCGTGTTCCTGATCTCGCCGTTTGTTGGCCGTa                                                                                                     <  2:583578/150‑1 (MQ=255)
                                                   cTGGCCTCCACCTGGCAGGGCATTCGCGCCGCTGAACAGCTGGCAAAAGAAGGCATTAACTGTAACCTGACGCTGCTGTTCTCCTTCGCTCAGGCTCGCGCTTGCGCCGAAGCGGGCGTCTTCCTGATTTCGCCGTTCGCTGGCCGTacc                                                                                                   >  2:543729/1‑148 (MQ=255)
                                                       cctccaCCTGGCAGGGCATTCGCGCCGCTGAACAGCTGGAAAAAGAAGGCATTAACTGTAACCTGACGCTGCTGTTCTCCTTCGCTCAGGCTCGCGCTTGCGCCGAAGCGGGCGTCTTCCTGATTTCGCCGTTCGTTGGCCGTATCCtcg                                                                                               <  1:1148130/145‑3 (MQ=255)
                                                       cctccaCCTGGCAGGGCATTCGCGCCGCTGAACAGCTGGAAAAAGAAGGCATTAACTGTAACCTGACGCTGCTGTTCTCCTTCGCTCAGGCTCGCGCTTGCGCCGAAGCGGGCGTCTTCCTGATTTCGCCGTTCGTTGGCCGTATCCtc                                                                                                >  2:120225/6‑148 (MQ=255)
                                                        ctccaCCTGGCAGGGCATTCGCGCCGCTGAACAGCTGGAAAAAGAAGGCATTAACTGTAACCTGACGCTGCTGTTCTCCTTCGCTCAGGCTCGCGCTTGCGCCGAAGCGGGCGTCTTCCTGATTTCGCCGTtcgt                                                                                                             >  1:401349/5‑135 (MQ=255)
                                                        ctccaCCTGGCAGGGCATTCGCGCCGCTGAACAGCTGGAAAAAGAAGGCATTAACTGTAACCTGACGCTGCTGTTCTCCTTCGCTCAGGCTCGCGCTTGCGCCGAAGCGGGCGTCTTCCTGATTTCGCCGTTCGTTGGCCGTATCCTCGa                                                                                              >  1:227386/5‑150 (MQ=255)
                                                         tccaCCTGGCAGGGCATTCGCGCCGCTGAACAGCTGGAAAAAGAAGGCATTAACTGTAACCTGACGCTGCTGTTCTCCTTCGCTCAGGCTCGCGCTTGCGCCGAAGCGGGCGTCTTCCTGATTTCGCcgttcgtt                                                                                                            <  2:401349/132‑1 (MQ=255)
                                                         tCTACCTGGCAGGGTATCCGTGCTGCAGAACAGCTGGAAAAAGAAGGCATCAACTGTAACCTGACCCTGCTGTTCTCCTTCGCTCAGGCTCGTGCTTGTGCGGAAGCGGGCGTGTTCCTGATCTCGCCGTTTGTTGGCCGTATTCTTGAc                                                                                             >  1:769300/1‑150 (MQ=255)
                                                          ccaCCTGGCAGGGCATTCGCGCCGCTGAACAGCTGGAAAAAGAAGGCATTAACTGTAACCTGACGCTGCTGTTCTCCTTCGCTCAGGCTCGCGCTTGCGCCGAAGCGGGCGTCTTCCTGATTTCGCCGTTCGTTGGCCGTATCCTCGACt                                                                                            >  1:308508/3‑150 (MQ=255)
                                                           caCCTGGCAGGGCATTCGCGCCGCTGAACAGCTGGAAAAAGAAGGCATTAACTGTAACCTGACGCTGCTGTTCTCCTTCGCTCAGGCTCGCGCTTGCGCCGAAGCGGGCGTCTTCCTGATTTCGCCGTTCGTTGGCCGTATCCTCGACt                                                                                            <  2:1093729/148‑1 (MQ=255)
                                                           caCCTGGCAGGGCATTCGCGCCGCTGAACAGCTGGAAAAAGAAGGCATTAACTGTAACCTGACGCTGCTGTTCTCCTTCGCTCAGGCTCGCGCTTGCGCCGAAGCGGGCGTCTTCCTGATTTCGCCGTTCGTTGGCCGTATCCTCGACt                                                                                            <  2:499941/148‑1 (MQ=255)
                                                           caCCTGGCAGGGCATTCGCGCCGCTGAACAGCTGGAAAAAGAAGGCATTAACTGTAACCTGACGCTGCTGTTCTCCTTCGCTCAGGCTCGCGCTTGCGCCGAAGCGGGCGTCTTCCTGATTTCGCCGTTCGTTGGCCGTATCCTCGACTg                                                                                           >  2:762904/2‑150 (MQ=255)
                                                             ccTGGCAGGGCATTCGCGCCGCTGAACAGCTGGAAAAAGAAGGCATTAACTGTAACCTGACGCTGCTGTTCTCCTTCGCTCAGGCTCGCGCTTGCGCCGAAGCGGGCGTCTTCCTGATTTCGCCGTTCGTTGGCCGTATCCTCGACTgg                                                                                          >  1:823405/1‑149 (MQ=255)
                                                              cTGGCAGGGCATTCGCGCCGCTGAACAGCTGGAAAAAGAAGGCATTAACTGTAACCTGACGCTGCTGTTCTCCTTCGCTCAGGCTCGCGCTTGCGCCGAAGCGGGCGTCTTCCTGATTTCGCCGTTCGTTGGCCGTATCCTCGACTGGTa                                                                                        >  2:946555/1‑150 (MQ=255)
                                                              cTGGCAGGGCATTCGCGCCGCTGAACAGCTGGAAAAAGAAGGCATTAACTGTAACCTGACGCTGCTGTTCTCCTTCGCTCAGGCTCGCGCTTGCGCCGAAGCGGGCGTCTTCCTGATTTCGCCGTTCGTTGGCCGTATCCTCGACTGGTa                                                                                        >  1:535215/1‑150 (MQ=255)
                                                                   aGGGTATCCGTGCTGCAGAACAGCTGGAAAAAGAAGGCATCAACTGTAACCTGACCCTGCTGTTCTCCTTCGCTCAGGCTCGTGCTTGTGCGGAAGCGGGCGTGTTCCTGATCTCGCCGTTTGTTGGCCGTATTCTTGACTGGTACAAAg                                                                                   <  1:749744/150‑1 (MQ=255)
                                                                   aGGGCATTCGCGCCGCTGAACAGCTGGAAAAAGAAGGCATTAACTGTAACCTGACGCTGCTGTTCTCCTTCGCTCAGGCTCGCGCTTGCGCCGAAGCGGGCGTCTTCCTGATTTCGCCGTTCGTTGGCCGTATCCTCGACTGGTACAAAg                                                                                   >  1:991476/1‑150 (MQ=255)
                                                                     ggTATCCGTGATGCAGAACAGCTGGAAAAAGAAGGCATCAACTGTAACCTGACCCTGCTGTTCTCCTTCGCTCAGGCTCGTGCTTGTGCGGAAGCGGGCGTGTTCCTGATCTCGCCGTTTGTTGGCCGTATTCTTGACTGGTACAAAGCg                                                                                 <  1:1007829/150‑1 (MQ=255)
                                                                          ccGTGCTGCAGAACAGCTGGAAAAAGAAGGCATCAACTGTAACCTGACCCTGCTGTTCTCCTTCGCTCAGGCTCGTGCTTGTGCGGAAGCGGGCGTGTTCCTGATCTCGCCGTTTGTTGGCCGTATTCTTGACTGGTACAAAGCGAATAc                                                                            >  2:336888/1‑150 (MQ=255)
                                                                            gTGCTGCAGAACAGCTGGAAAAAGAAGGCATCAACTGTAACCTGACCCTGCTGTTCTCCTTCGCTCAGGCTCGTGCTTGTGCGGAAGCGGGCGTGTTCCTGATCTCGCCGTTTGTTGGCCGTATTCTTGACTGGTACAAAGCGAATAcc                                                                           >  1:254516/1‑149 (MQ=255)
                                                                            gTGCTGCAGAACAGCTGGAAAAAGAAGGCATCAACTGTAACCTGACCCTGCTGTTCTCCTTCGCTCAGGCTCGTGCTTGTGCGGAAGCGGGCGTGTTCCTGATCTCGCCGTTTGTTGGCCGTATTCTTGACTGGTACAAAGCGAATAcc                                                                           >  2:717136/1‑149 (MQ=255)
                                                                              gctgcAGAACAGCTGGAAAAAGAAGGCATCAACTGTAACCTGACCCTGCTGTTCTCCTTCGCTCAGGCTCGTGCTTGTGCGGAAGCGGGCGTGTTCCTGATCTCGCCGTTTGTTGGCCGTATTCTTGACTGGTACAAAGCGAATACCGa                                                                         >  1:555750/1‑149 (MQ=255)
                                                                              gccgctgAACAGCTGGAAAAAGAAGGCATTAACTGTAACCTGACGCTGCTGTTCTCCTTCGCTCAGGCTCGCGCTTGCGCCGAAGCGGGCGTCTTCCTGATTTCGCCGTTAGTTGGCCGTATCCTCGACTGGTACAAAGCCAACACCGa                                                                         >  2:227932/7‑149 (MQ=255)
                                                                               ccgctgAACAGCTGGAAAAAGAAGGCATTAACTGTAACCTGACGCTGCTGTTCTCCTTCGCTCAGGCTCGCGCTTGCGCCGAAGCGGGCGTCTTCCTGATTTCGCCGTTCGTTGGCCGTATCCTCGACTGGTACAAAGCCAACACCGaca                                                                       <  2:253992/145‑3 (MQ=255)
                                                                                tgcAGAACAGCTGGAAAAAGAAGGCATCAACTGTAACCTGACCCTGCTGTTCTCCTTCGCTCAGGCTCGTGCTTGTGCGGAAGCGGGCGTGTTCCTGATCTCGCCGTTTGTTGGCCGTATTCTTGACTGGTACAAAGCGAATACCGAt                                                                        >  2:905955/1‑148 (MQ=255)
                                                                                cgctgAACAGCTGGAAAAAGAAGGCATTAACTGTAACCTGACGCTGCTGTTCTCCTTCGCTCAGGCTCGCGCTTGCGCCGAAGCGGGCGTCTTCCTGATTTCGCCGTTCGTTGGCCGTATCCTCGACTGGTACAAAGCCAACACCGac                                                                        >  2:1135411/5‑147 (MQ=255)
                                                                                 gctgAACAGCTGGAAAAAGAAGGCATTAACTGTAACCTGACGCTGCTGTTCTCCTTCGCTCAGGCTCGCGCTTGCGCCGAAGCGGGCGTCTTCCTGATTTCGCCGTTCGTTGGCCGTATCCTCGACTGGTACAAAGCCAACACCGACaag                                                                     <  2:1078511/147‑1 (MQ=255)
                                                                                  ctgAACAGCTGGAAAAAGAAGGCATTAACTGTAACCTGACGCTGCTGTTCTCCTTCGCTCAGGCTCGCGCTTGCGCCGAAGCGGGCGTCTTCCTGATTTCGCCGTTCGTTGGCCGTATCCTCGACTGGTACAAAGCCAACACCGACaaga                                                                    <  1:487828/148‑1 (MQ=255)
                                                                                  cAGAACAGCTGGAAAAAGAAGGCATCAACTGTAACCTGACCCTGCTGTTCTCCTTCGCTCAGGCTCGTGCTTGTGCGGAAGCGGGCGTGTTCCTGATCTCGCCGTTTGTTGGCCGTATTCTTGACTGGTACAAAGCGAATACCGATaaga                                                                    <  1:1089560/150‑1 (MQ=255)
                                                                                   tgAACAGCTGGAAAAAGAAGGCATTAACTGTAACCTGACGCTGCTGTTCTCCTTCGCTCAGGCTCGCGCTTGCGCCGAAGCGGGCGTCTTCCTGATTTCGCCGTTCGTTGGCCGTATCCTCGACTGGTACAAAGCCAACACCGACaaga                                                                    >  2:1128087/2‑149 (MQ=255)
                                                                                   tgAACAGCTGGAAAAAGAAGGCATTAACTGTAACCTGACGCTGCTGTTCTCCTTCGCTCAGGCTCGCGCTTGCGCCGAAGCGGGCGTCTTCCTGATTTCGCCGTTCGTTGGCCGTATCCTCGACTGGTACAAAGCCAACACCGACaaga                                                                    >  1:69941/2‑149 (MQ=255)
                                                                                     aaCAGCTGGAAAAAGAAGGCATTAACTGTAACCTGACGCTGCTGTTCTCCTTCGCTCAGGCTCGCGCTTGCGCCGAAGCGGGCGTCTTCCTGATTTCGCCGTTCGTTGGCCGTATCCTCGACTGGTACAAAGCCAACACCGACaagaaag                                                                 <  1:52560/150‑1 (MQ=255)
                                                                                       cAGCTGGAAAAAGAAGGCATTAACTGTAACCTGACGCTGCTGTTCTCCTTCGCTCAGGCTCGCGCTTGCGCCGAAGCGGGCGTCTTCCTGATTTCGCCGTTCGTTGGCCGTATCCTCGACTGGTACAAAGCCAACACCGACAAGAAagag                                                               >  1:331839/1‑150 (MQ=255)
                                                                                       cAGCTGGAAAAAGAAGGCATTAACTGTAACCTGACGCTGCTGTTCTCCTTCGCTCAGGCTCGCGCTTGCGCCGAAGCGGGCGTCTTCCTGATTTCGCCGTTCGTTGGCCGTATCCTCGACTGGTACAAAGCCAACACCGACAAGAAagag                                                               <  2:811658/150‑1 (MQ=255)
                                                                                        aGCTGGAAAAAGAAGGCATTAACTGTAACCTGACGCTGCTGTTCTCCTTCGCTCAGGCTCGCGCTTGCGCCGAAGCGGGCGTCTTCCTGATTTCGCCGTTCGTTGGCCGTATCCTCGACTGGTACAAAGCCAACACCGACAAGAAagag                                                               >  2:987068/1‑149 (MQ=255)
                                                                                          cTGGAAAAAGTAGGCATCAACTGTAACCTGACCCTGCTGTTCTCCTTCGCTCAGGCTCGTGCTTGTGCGGAAGCGGGCGTGTTCCTGATCTCGCCGTTTGTTGGCCGTATTCTTGACTGGTACAAAGCGAATACCGATAAGAAAGAGTAc                                                            <  1:248138/150‑1 (MQ=255)
                                                                                          cTGGAAAAAGAAGGCATTAACTGTAACCTGACGCTGCTGTTCTCCTTCGCTCAGGCTCGCGCTTGCGCCGAAGCGGGCGTCTTCCTGATTTCGCCGTTCGTTGGCCGTATCCTCGACTGGTACAAAGCCAACACCGACAAGAAAGAGTAc                                                            <  2:641708/150‑1 (MQ=255)
                                                                                          cTGGAAAAAGAAGGCATTAACTGTAACCTGACGCTGCTGTTCTCCTTCGCTCAGGCTCGCGCTTGCGCCGAAGCGGGCGTCTTCCTGATTTCGCCGTTCGTTGGCCGTATCCTCGACTGGTACAAAGCCAACACCGACAAGAAAGAGTAc                                                            >  1:921305/1‑150 (MQ=255)
                                                                                             gAAAAAGAAGGCATCAACTGTAACCTGACCCTGCTGTTCTCCTTCGCTCAGGCTCGTGCTTGTGCGGAAGCGGGCGTGTTCCTGATCTCGCCGTTTGTTGGCCGTATTCTTGACTGGTACAAAGCGAATACCGATAAGAAAGAGTACGc                                                          <  2:528366/149‑1 (MQ=255)
                                                                                               aaaaGAAGGCATTAACTGTAACCTGACGCTGCTGTTCTCCTTCGCTCAGGCTCGCGCTTGCGCCGAAGCGGGCGTCTTCCTGATTTCGCCGTTCGTTGGCCGTATCCTCGACTGGTACAAAGCCAACACCGACAAGAAAGAGTACGCAcc                                                       <  2:1028648/150‑1 (MQ=255)
                                                                                                 aagaagGCATTAACTGTAACCTGACGCTGCTGTTCTCCTTCGCTCAGGCTCGCGCTTGCGCCGAAGCGGGCGTCTTCCTGATTTCGCCGTTCGTTGGCCGTATCCTCGACTGGTACAAAGCCAACACCGACAAGAAAGAGTACGCACCgg                                                     <  1:743061/150‑1 (MQ=255)
                                                                                                  agaagGCATCAACTGTAACCTGACCCTGCTGTTCTCCTTCGCTCAGGCTCGTGCTTGTGCGGAAGCGGGCGTGTTCCTGATCTCGCCGTTTGTTGGCCGTATTCTTGACTGGTACAAAGCGAATACCGATAAGAAAGAGTACGCTCCGGc                                                    <  2:624994/150‑1 (MQ=255)
                                                                                                    aagGCATTAACTGTAACCTGACGCTGCTGTTCTCCTTCGCTCAGGCTCGCGCTTGCGCCGAAGCGGGCGTCTTCCTGATTTCGCCGTTCGTTGGCCGTATCCTCGACTGGTACAAAGCCAACACCGACAAGAAAGAGTACGCACCgg                                                     >  2:496012/1‑147 (MQ=255)
                                                                                                     agGCATCAACTGTAACCTGACCCTGCTGTTCTCCTTCGCTCAGGCTCGTGCTTGTGCGGAAGCGGGCGTGTTCCTGATCTCGCCGTTTGTTGGCCGTATTCTTGACTGGTACAAAGCGAATACCGATAAGAAAGAGTACGCTCCGGCag                                                  >  2:1107345/1‑149 (MQ=255)
                                                                                                       gCATTAACTGTAACCTGACGCTGCTGTTCTCCTTCGCTCAGGCTCGCGCTTGCGCCGAAGCGGGCGTCTTCCTGATTTCGCCGTTCGTTGGCCGTATCCTCGACTGGTACAAAGCCAACACCGACAAGAAAGAGTACGCACCGGCag                                                  >  2:730865/1‑147 (MQ=255)
                                                                                                         attaaCTGTAACCTGACGCTGCTGTTCTCCTTCGCTCAGGCTCGCGCTTGCGCCGAAGCGGGCGTCTTCCTGATTTCGCCGTTCGTTGGCCGTATCCTCGACTGGTACAAAGCCAACACCGACAAGAAAGAGTACGCACCGGCag                                                  >  1:1067948/4‑145 (MQ=255)
                                                                                                         attaaCTGTAACCTGACGCTGCTGTTCTCCTTCGCTCAGGCTCGCGCTTGCGCCGAAGCGGGCGTCTTCCTGATTTCGCCGTTCGTTGGCCGTATCCTCGACTGGTACAAAGCCAACACCGACAAGAAAGAGTACGCACCGGCAGAAGAt                                             <  1:805406/147‑1 (MQ=255)
                                                                                                          ttaaCTGTAACCTGACGCTGCTGTTCTCCTTCGCTCAGGCTCGCGCTTGCGCCGAAGCGGGCGTCTTCCTGATTTCGCCGTTCGTTGGCCGTATCCTCGACTGGTACAAAGCCAACACCGACAAGAAAGAGTACGCACCGGCAGAAGATc                                            <  1:354409/148‑1 (MQ=255)
                                                                                                             aCTGTAACCTGACGCTGCTGTTCTCCTTCGCTCAGGCTCGCGCTTGCGCCGAAGCGGGCGTCTTCCTGATTTCGCCGTTCGTTGGCCGTATCCTCGACTGGTACAAAGCCAACACCGACAAGAAAGAGTACGCACCGGCAGAAGATCCg                                          <  1:1161944/149‑1 (MQ=255)
                                                                                                              cTGTAACCTGACCCTGCTGTTCTCCTTCGCTCAGGCTCGTGCTTGTGCGGAAGCGGGCGTGTTCCTGATCTCGCCGTTTGTTGGCCGTATTCTTGACTGGTACAAAGCGAATACCGATAAGAAAGAGTACGCTCCGGCAGAAGATCCggg                                        >  1:930989/1‑150 (MQ=255)
                                                                                                                gTAACCTGACGCTGCTGTTCTCCTTCGCTCAGGCTCGCGCTTGCGCCGAAGCGGGCGTCTTCCTGATTTCGCCGTTCGTTGGCCGTATCCTCGACTGGTACAAAGCCAACACCGACAAGAAAGAGTACGCACCGGCAGAAGATCCGGGCg                                      >  2:676016/1‑150 (MQ=255)
                                                                                                                   aCCTGACGCTGCTGTTCTCCTTCGCTCAGGCTCGCGCTTGCGCCGAAGCGGGCGTCTTCCTGATTTCGCCGTTCGTTGGCCGTATCCTCGACTGGTACAAAGCCAACACCGACAAGAAAGAGTACGCACCGGCAGAAGATCCGGGCGTg                                    <  1:253196/149‑1 (MQ=255)
                                                                                                                    ccTGACGCTGCTGTTCTCCTTCGCTCAGGCTCGCGCTTGCGCCGAAGCGGGCGTCTTCCTGATTTCGCCGTTCGTTGGCCGTATCCTCGACTGGTACAAAGCCAACACCGACAAGAAAGAGTACGCACCGGCAGAAGATCCGGGCGTGGt                                  >  1:259078/1‑150 (MQ=255)
                                                                                                                     cTGACCCTGCTGTTCTCCTTCGCTCAGGCTCGTGCTTGTGCGGAAGCGGGCGTGTTCCTGATCTCGCCGTTTGTTGGCCGTATTCTTGACTGGTACAAAGCGAATACCGATAAGAAAGAGTACGCTCCGGCAGAAGATCCGGGCGTGGtt                                 <  1:905955/150‑1 (MQ=255)
                                                                                                                       gACCCTGCTGTTCTCCTTCGCTCAGGCTCGTGCTTGTGCGGAAGCGGGCGTGTTCCTGATCTCGCCGTTTGTTGGCCGTATTCTTGACTGGTACAAAGCGAATACCGATAAGAAAGAGTACGCTCCGGCAGAAGATCCGGGCGTGGTTTc                               >  2:941380/1‑150 (MQ=255)
                                                                                                                              ctgTTCTCCTTCGCTCAGGCTCGCGCTTGCGCCGAAGCGGGCGTCTTCCTGATTTCGCCGTTCGTTGGCCGTATCCTCGACTGGTACAAAGCCAACACCGACAAGAAAGAGTACGCACCGGCAGAAGATCCGGGCGTGGTTTCCGtcagc                        >  2:836858/1‑146 (MQ=255)
                                                                                                                               tgTTCTCCTTCGCTCAGGCTCGTGCTTGTGCGGAAGCGGGCGTGTTCCTGATCTCGCCGTTTGTTGGCCGTATTCTTGACTGGTACAAAGCGAATACCGATAAGAAAGAGTACGCTCCGGCAGAAGATCCGGGCGTGGTTTCTGTATCt                        >  2:117719/1‑149 (MQ=255)
                                                                                                                                       ttCGCTCAGGCTCGTGCTTGTGCGGAAGCGGGCGTGTTCCTGATCTCGCCGTTTGTTGGCCGTATTCTTGACTGGTACAAAGCGAATACCGATAAGAAAGAGTACGCTCCGGCAGAAGATCCGGGCGTGGTTTCTGTATCTGAAATCTAc               >  2:593328/1‑150 (MQ=255)
                                                                                                                                         cGCTCAGGCTCGTGCTTGTGCGGAAGCGGGCGTGTTCCTGATCTCGCCGTTTGTTGGCCGTATTCTTGACTGGTACAAAGCGAATACCGATAAGAAAGAGTACGCTCCGGCAGAAGATCCGGGCGTGGTTTCTGTATCTGAAATCTAcc              <  1:86830/149‑1 (MQ=255)
                                                                                                                                          gCTCAGGCTCGCGCTTGCGCCGAAGCGGGCGTCTTCCTGATTTCGCCGTTCGTTGGCCGTATCCTCGACTGGTACAAAGCCAACACCGACAAGAAAGAGTACGCACCGGCAGAAGATCCGGGCGTGGTTTCCGTCAGCGAAATCTAcga             <  1:394302/149‑3 (MQ=255)
                                                                                                                                              aGGCTCGTGCTTGTGCGGAAGCGGGCGTGTTCCTGATCTCGCCGTTTGTTGGCCGTATTCTTGACTGGTACAAAGCGAATACCGATAAGAAAGAGTACGCTCCGGCAGAAGATCCGGGCGTGGTTTCTGTATCTGAAATCTACCAGtac         <  1:139655/149‑1 (MQ=255)
                                                                                                                                               ggCTCGCGCTTGCGCCGAAGCGGGCGTGTTCCTGATTTCGCCGTTCGTTGGCCGTATCCTCGACTGGTACAAAGCCAACACCGACAAGAAAGAGTACGCACCGGCAGAAGATCCGGGCGTGGTTTCCGTCAGCGAAATCTACGAGtatta       <  2:161465/150‑1 (MQ=255)
                                                                                                                                                 cTCGCGCTTGCGCCGAAGCGGGCGTCTTCCTGATTTCGCCGTTCGTTGGCCGTATCCTCGACTGGTACAAAGCCAACACCGACAAGAAAGAGTACGCACCGGCAGAAGATCCGGGCGTGGTTTCCGTCAGCGAAATCTACGAGtattac      >  2:238509/1‑149 (MQ=255)
                                                                                                                                                 cTCGCGCTTGCGCCGAAGCGGGCGTCTTCCTGATTTCGCCGTTCGTTGGCCGTATCCTCGACTGGTACAAAGCCAACACCGACAAGAAAGAGTACGCACCGGCAGAAGATCCGGGCGTGGTTTCCGTCAGCGAAATCTACGAGTATTACa     <  1:125370/150‑1 (MQ=255)
                                                                                                                                                    gTGCTTGTGCGGAAGCGGGCGTGTTCCTGATCTCGCCGTTTGTTGGCCGTATTCTTGACTGGTACAAAGCGAATACCGATAAGAAAGAGTACGCTCCGGCAGAAGATCCGGGCGTGGTTTCTGTATCTGAAATCTACCAGTACTACaaa   <  1:634668/149‑1 (MQ=255)
                                                                                                                                                    gTGCTTGTGCGGAAGCGGGCGTGTTCCTGATCTCGCCGTTTGTTGGCCGTATTCTTGACTGGTACAAAGCGAATACCGATAAGAAAGAGTACGCTCCGGCAGAAGATCCGGGCGTGGTTTCTGTATCTGAAATCTACCAGTACTACAAag  <  1:117719/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                    
ATCAAACTCTACAACGATGCTGGTATTAGCAACGATCGTATTCTGATCAAACTGGCTTCTACCTGGCAGGGTATCCGTGCTGCAGAACAGCTGGAAAAAGAAGGCATCAACTGTAACCTGACCCTGCTGTTCTCCTTCGCTCAGGCTCGTGCTTGTGCGGAAGCGGGCGTGTTCCTGATCTCGCCGTTTGTTGGCCGTATTCTTGACTGGTACAAAGCGAATACCGATAAGAAAGAGTACGCTCCGGCAGAAGATCCGGGCGTGGTTTCTGTATCTGAAATCTACCAGTACTACAAAG  >  CP009273/8583‑8880

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GATCAAACTCTACAACGATGCTGGTATTAGCAACGATCGTATTCTGATCAAACTGGCTTCTACCTGGCAGGGTATCCGTGCTGCAGAACAGCTGGAAAAAGAAGGCATCAACTGTAACCTGACCCTGCTGTTCTCCTTCGCTCAGGCTCGTGCTTGTGCGGAAGCGGGCGTGTTCCTGATCTCGCCGTTTGTTGGCCGTATTCTTGACTGGTACAAAGCGAATACCGATAAGAAAGAGTACGCTCCGGCAGAAGATCCGGGCGTGGTTTCTGTATCTGAAATCTACCAGTACTACAAAG  >  CP009273/8582‑8880
                                                                                                                                                      |                                                                                                                                                    
GATCAAACTCTACAACGATGCTGGTATTAGCAACGATCGTATTCTGATCAAACTGGCTTCTACCTGGCAGGGTATCCGTGCTGCAGAACAGCTGGAAAAAGAAGGCATCAACTGTAACCTGACCCTGCTGTTCTCCTTCGCTCAGGCTCGT                                                                                                                                                      >  SRR12117898.530432/1‑151 (MQ=60)
   caagctgtacaacgacgcgggcatcAGCAACGATCGCATTCTGATCAAACTGGCCTCCACCTGGCAGGGCATTCGCGCCGCTGAACAGCTGGAAAAAGAAGGCATTAACTGTAACCTGACGCTGCTGTTCTCCTTCGCTCAGGCTCGCGCT                                                                                                                                                   <  SRR12117898.547863/126‑1 (MQ=60)
    aagctgtacaacgacgcgggcatcAGCAACGATCGCATTCTGATCAAACTGGCCTCCACCTGGCAGGGCATTCGCGCCGCTGAACAGCTGGAAAAAGAAGGCATTAACTGTAACCTGACGCTGCTGTTCTCCTTCGCTCAGGCTCGCGCTT                                                                                                                                                  <  SRR12117898.1122201/127‑1 (MQ=60)
       CTGTACAACGACGCGGGCATCAGCAACGATCGCATTCTGATCAAACTGGCCTCCACCTGGCAGGGCATTCGCGCCGCTGAACAGCTGGAAAAAGAAGGCATTAACTGTAACCTGACGCTGCTGTTCTCCTTCGCTCAGGCTCGCGCTTGCG                                                                                                                                               >  SRR12117898.603465/1‑151 (MQ=60)
          TACAACGATGCTGGTATTAGCAACGATCGTATTCTGATCAAACTGGCTTCTACCTGGCAGGGTATCCGTGCTGCAGAACAGCTGGAAAAAGAAGGCATCAACTGTAACCTGACCCTGCTGTTCTCCTTCGCTCAGGCTCGTGCTTGTGCGG                                                                                                                                            >  SRR12117898.1185541/1‑151 (MQ=60)
          TACAACGACGCGGGCATCAGCAACGATCGCATTCTGATCAAACTGGCCTCCACCTGGCAGGGCATTCGCGCCGCTGAACAGCTGGAAAAAGAAGGCATTAACTGTAACCTGACGCTGCTGTTCTCCTTCGCTCAGGCTCGCGCTTGcgccg                                                                                                                                            >  SRR12117898.713562/1‑146 (MQ=60)
           ACAACGACGCGGGCATCAGCAACGATCGCATTCTGATCAAACTGGCCTCCACCTGGCAGGGCATTCGCGCCGCTGAACAGCTGGAAAAAGAAGGCATTAACTGTAACCTGACGCTGCTGTTCTCCTTCGCTCAGGCTCGCGCTTGcgccg                                                                                                                                            >  SRR12117898.644211/1‑145 (MQ=60)
             aacgacgcgggcatcAGCAACGATCGCATTCTGATCAAACTGGCCTCCACCTGGCAGGGCATTCGCGCCGCTGAACAGCTGGAAAAAGAAGGCATTAACTGTAACCTGACGCTGCTGTTCTCCTTCGCTCAGGCTCGCGCTTGCGCCGA                                                                                                                                           >  SRR12117898.437663/16‑149 (MQ=60)
             AACGATGCTGGTATTAGCAACGATCGTATTCTGATCAAACTGGCTTCTACCTGGCAGGGTATCCGTGCTGCAGAACAGCTGGAAAAAGAAGGCATCAACTGTAACCTGACCCTGCTGTTCTCCTTCGCTCAGGCTCGTGCTTGTGCGGAAG                                                                                                                                         <  SRR12117898.660278/151‑1 (MQ=60)
              acgacgcgggcatcAGCAACGATCGCATTCTGATCAAACTGGCCTCCACCTGGCAGGGCATTCGCGCCGCTGAACAGCTGGAAAAAGAAGGCATTAACTGTAACCTGACGCTGCTGTTCTCCTTCGCTCAGGCTCGCGCTTGCGCCGAAGC                                                                                                                                        <  SRR12117898.615029/137‑1 (MQ=60)
              acgacgcgggcatcAGCAACGATCGCATTCTGATCAAACTGGCCTCCACCTGGCAGGGCATTCGCGCCGCTGAACAGCTGGAAAAAGAAGGCATTAACTGTAACCTGACGCTGCTGTTCTCCTTCGCTCAGGCTCGCGCTTGCGCCGAAGC                                                                                                                                        <  SRR12117898.644262/137‑1 (MQ=60)
               ggactcgggcatcAGCAACGATCGCATTCTGATCAAACTGGCCTCCACCTGGCAGGGCATTCGCGCCGCTGAACAGCTGGAAAAAGAAGGCATTAACTGTAACCTGACGCTGCTGTTCTCCTTCGCTCAGGCTCGCGCTTGCGCCGAAGCG                                                                                                                                       <  SRR12117898.1197026/138‑1 (MQ=60)
               cgacgcgggcatcAGCAACGATCGCATTCTGATCAAACTGGCCTCCACCTGGCAGGGCATTCGCGCCGCTGAACAGCTGGAAAAAGAAGGCATTAACTGTAACCTGACGCTGCTGTTCTCCTTCGCTCAGGCTCGCGCTTGCGCCGAAGCG                                                                                                                                       <  SRR12117898.1160888/138‑1 (MQ=60)
               cgacgcgggcatcAGCAACGATCGCATTCTGATCAAACTGGCCTCCACCTGGCAGGGCATTCGCGCCGCTGAACAGCTGGAAAAAGAAGGCATTAACTGTAACCTGACGCTGCTGTTCTCCTTCGCTCAGGCTCGCGCTTGCGCCGAAGCG                                                                                                                                       <  SRR12117898.66100/138‑1 (MQ=60)
                gacgcgggcatcAGCAACGATCGCATTCTGATCAAACTGGCCTCCACCTGGCAGGGCATTCGCGCCGCTGAACAGCTGGAAAAAGAAGGCATTAACTGTAACCTGACGCTGCTGTTCTCCTTCGCTCAGGCTCGCGCTTGCGCCGAAGCGG                                                                                                                                      <  SRR12117898.418293/139‑1 (MQ=60)
                gacgcgggcatcAGCAACGATCGCATTCTGATCAAACTGGCCTCCACCTGGCAGGGCATTCGCGCCGCTGAACAGCTGGAAAAAGAAGGCATTAACTGTAACCTGACGCTGCTGTTCTCCTTCGCTCAGGCTCGCGCTTGCGCCGAAGCGG                                                                                                                                      <  SRR12117898.729364/139‑1 (MQ=60)
                    cgggcatcAGCAACGATCGCATTCTGATCAAACTGGCCTCCACCTGGCAGGGCATTCGCGCCGCTGAACAGCTGGAAAAAGAAGGCATTAACTGTAACCTGACGCTGCTGTTCTCCTTCGCTCAGGCTCGCGCTTGCGCCGAAGCGGGCGT                                                                                                                                  >  SRR12117898.643723/9‑151 (MQ=60)
                     TGGTATTAGCAACGATCGTATTCTGATCAAACTGGCTTCTACCTGGCAGGGTATCCGTGCTGCAGAACAGCTGGAAAAAGAAGGCATCAACTGTAACCTGACCCTGCTGTTCTCCTTCGCTCAGGCTCGTGCTTGTGC                                                                                                                                              >  SRR12117898.450091/1‑138 (MQ=60)
                      GGCATCAGCAACGATCGCATTCTGATCAAACTGGCCTCCACCTGGCAGGGCATTCGCGCCGCTGAACAGCTGGAAAAAGAAGGCATTAACTGTAACCTGACGCTGCTGTTCTCCTTCGCTCAGGCTCGCGCTTGCGCCGAAGCGGGCGTCT                                                                                                                                >  SRR12117898.924484/1‑151 (MQ=60)
                         ATCAGCAACGATCGCATTCTGATCAAACTGGCCTCCACCTGGCAGGGCATTCGCGCCGCTGAACAGCTGGAAAAAGAAGGCATTAACTGTAACCTGACGCTGCTGTTCTCCTTCGCTCAGGCTCGCGCTTGCGCCGAAGCGGGCGTCTTCC                                                                                                                             >  SRR12117898.631100/1‑151 (MQ=60)
                         ATCAGCAACGATCGCATTCTGATCAAACTGGCCTCCACCTGGCAGGGCATTCGCGCCGCTGAACAGCTGGAAAAAGAAGGCATTAACTGTAACCTGACGCTGCTGTTCTCCTTCGCTCAGGCTCGCGCTTGCGCCGAAGCGGGCGTCTTCC                                                                                                                             >  SRR12117898.255670/1‑151 (MQ=60)
                            AGCAACGATCGTATTCTGATCAAACTGGCTTCTACCTGGCAGGGTATCCGTGCTGCAGAACAGCTGGAAAAAGAAGGCATCAACTGTAACCTGACCCTGCTGTTCTCCTTCGCTCAGGCTCGTGCTTGTGCGGAAGCGGGCGTGTTCCTG                                                                                                                           <  SRR12117898.597060/150‑1 (MQ=60)
                            AGCAACGATCGCATTCTGATCAAACTGGCCTCCACCTGGCAGGGCATTCGCGCCGCTGAACAGCTGGAAAAAGAAGGCATTAACTGTAACCTGACGCTGCTGTTCTCCTTCGCTCAGGCTCGCGCTTGGGCCGAAGCGGGCGTCTTCCTG                                                                                                                           >  SRR12117898.99562/1‑150 (MQ=60)
                              CAACGATCGCATTCTGATCAAACTGGCCTCCACCTGGCAGGGCATTCGCGCCGCTGAACAGCTGGAAAAAGAAGGCATTAACTGTAACCTGACGCTGCTGTTCTCCTTCGCTCAGGCTCGCGCTTGCGCCGAAGCGGGCGTCTTCCTGATT                                                                                                                        <  SRR12117898.663763/151‑1 (MQ=60)
                              CAACGATCGCATTCTGATCAAACTGGCCTCCACCTGGCAGGGCATTCGCGCCGCTGAACAGCTGGAAAAAGAAGGCATTAACTGTAACCTGACGCTGCTGTTCTCCTTCGCTCAGGCTCGCGCTTGCGCCGAAGCGGGCGTCTTC                                                                                                                              >  SRR12117898.1101081/1‑145 (MQ=60)
                               AACGATCGTATTCTGATCAAACTGGCTTCTACCTGGCAGGGTATCCGTGCTGCAGAACAGCTGGAAAAAGAAGGCATCAACTGTAACCTGACCCTGCTGTTCTCCTTCGCTCAGGCTCGTGCTTGTGCGGAAGCGGGCGTGTTCCTGATCT                                                                                                                       >  SRR12117898.688442/1‑151 (MQ=60)
                               AACGATCGTATTCTGATCAAACTGGCTTCTACCTGGCAGGGTATCCGTGCTGCAGAACAGCTGGAAAAAGAAGGCATCAACTGTAACCTGACCCTGCTGTTCTCCTTCGCTCAGGCTCGTGCTTGTGCGGAAGCGGGCGTGTTCCTGATCT                                                                                                                       <  SRR12117898.249045/151‑1 (MQ=60)
                                ACGATCGCATTCTGATCAAACTGGCCTCCACCTGGCAGGGCATTCGCGCCGCTGAACAGCTGGAAAAAGAAGGCATTAACTGTAACCTGACGCTGCTGTTCTCCTTCGCTCAGGCTCGCGCTTGCGCCGAAGCGGGCGTCTTCCTGATTTC                                                                                                                      >  SRR12117898.183800/1‑151 (MQ=60)
                                 CGATCGTATTCTGATCAAACTGGCTTCTACCTGGCAGGGTATCCGTGCTGCAGAACAGCTGGAAAAAGAAGGCATCAACTGTAACCTGACCCTGCTGTTCTCCTTCGCTCAGGCTCGTGCTTGTGCGGAAGCGGGCGTGTTCCTGATCTCG                                                                                                                     <  SRR12117898.495777/151‑1 (MQ=60)
                                 CGATCGCATTCTGATCAAACTGGCCTCCACCTGGCAGGGCATTCGCGCCGCTGAACAGCTGGAAAAAGAAGGCATTAACTGTAACCTGACGCTGCTGTTCTCCTTCGCTCAGGCTCGCGCTTGCGCCGAAGCGGGCGTCTTCCTGATTTCG                                                                                                                     >  SRR12117898.402598/1‑151 (MQ=60)
                                 CGATCGCATTCTGATCAAACTGGCCTCCACCTGGCAGGGCATTCGCGCCGCTGAACAGCTGGAAAAAGAAGGCATTAACTGTAACCTGACGCTGCTGTTCTCCTTCGCTCAGGCTCGCGCTTGCGCCGAAGCGGGCGTCTTCCTGATTTCG                                                                                                                     >  SRR12117898.322549/1‑151 (MQ=60)
                                   ATCGCATTCTGATCAAACTGGCCTCCACCTGGCAGGGCATTCGCGCCGCTGAACAGCTGGAAAAAGAAGGCATTAACTGTAACCTGACGCTGCTGTTCTCCTTCGCTCAGGCTCGCGCTTGCGCCGAAGCGGGCGTCTTCCTGATTTCGCC                                                                                                                   <  SRR12117898.582829/151‑1 (MQ=60)
                                   ATCGCATTCTGATCAAACTGGCCTCCACCTGGCAGGGCATTCGCGCCGCTGAACAGCTGGAAAAAGAAGGCATTAACTGTAACCTGACGCTGCTGTTCTCCTTCGCTCAGGCTCGCGCTTGCGCCGAAGCGGGCGTCTTCCTGATTTCGCC                                                                                                                   <  SRR12117898.1161375/151‑1 (MQ=60)
                                     CGTATTCTGATCAAACTGGCTTCTACCTGGCAGGGTATCCGTGCTGCAGAACAGCTGGAAAAAGAAGGCATCAACTGTAACCTGACCCTGCTGTTCTCCTTCGCTCAGGCTCGTGCTTGTGCGGAAGCGGGCGTGTTCCTGATCTCGCCG                                                                                                                  <  SRR12117898.381010/150‑1 (MQ=60)
                                      GCATTCTGATCAAACTGGCCTCCACCTGGCAGGGCATTCGCGCCGCTGAACAGCTGGAAAAAGAAGGCATTAACTGTAACCTGACGCTGCTGTTCTCCTTCGCTCAGGCTCGCGCTTGCGCCGAAGCGGGCGTCTTCCTGATTTCGCCGTT                                                                                                                >  SRR12117898.750592/1‑151 (MQ=60)
                                          TCTGATCAAACTGGCCTCCACCTGGGAGGGCATTCGCGCCGCTGAACAGCTGGAAAAAGAAGGCATTAACTGTAACCTGACGCTGCTGTTCTCCTTCGCTCAGGCTCGCGCTTGCGCCGAAGCGGGCGTCTTCCTGATTTCGCCGTTCGT                                                                                                             <  SRR12117898.1096695/150‑1 (MQ=60)
                                             GATCAAACTGGCTTCTACCTGGCAGGGTATCCGTGCTGCAGAACAGCTGGAAAAAGAAGGCATCAACTGTAACCTGACCCTGCTGTTCTCCTTCGCTCAGGCTCGTGCTTGTGCGGAAGCGGGCGTGTTCCTGATCTCGCCGTTTGTTGGC                                                                                                         >  SRR12117898.122349/1‑151 (MQ=60)
                                             GATCAAACTGGCTTCTACCTGGCAGGGTATCCGTGCTGCAGAACAGCTGGAAAAAGAAGGCATCAACTGTAACCTGACCCTGCTGTTCTCCTTCGCTCAGGCTCGTGCTTGTGCGGAAGCGGGCGTGTTCCTGATCTCGCCGTTTGTTGG                                                                                                          <  SRR12117898.177781/150‑1 (MQ=60)
                                             GATCAAACTGGCCTCCACCTGGCAGGGCATTCGCGCCGCTGAACAGCTGGAAAAAGAAGGCATTAACTGTAACCTGACGCTGCTGTTCTCCTTCGCTCAGGCTCGCGCTTGCGCCGAAGCGGGCGTCTTCCTGATTTCGCCGTTCGTTGGC                                                                                                         >  SRR12117898.460161/1‑151 (MQ=60)
                                              ATCAAACTGGCCTCCACCTGGCAGGGCATTCGCGCCGCTGAACAGCTGGAAAAAGAAGGCATTAACTGTAACCTGACGCTGCTGTTCTCCTTCGCTCAGGCTCGCGCTTGCGCCGAAGCGGGCGTCTTCCTGATTTCGCCGTTCGTTGGCC                                                                                                        >  SRR12117898.386781/1‑151 (MQ=60)
                                                   ACTGGCCTCCACCTGGCAGGGCATTCGCGCCGCTGAACAGCTGGAAAAAGAAGGCATTAACTGTAACCTGACGCTGCTGTTCTCCTTCGCTCAGGCTCGCGCTTGCGCCGAAGCGGGCGTCTTCCTGATTTCGCCGTTCGTTGGCCGTATC                                                                                                   <  SRR12117898.533187/151‑1 (MQ=60)
                                                       gcctccacctggcagggcattcgcgccgctGAACAGCTGGAAAAAGAAGGCATTAACTGTAACCTGACGCTGCTGTTCTCCTTCGCTCAGGCTCGCGCTTGCGCCGAAGCGGGCGTCTTCCTGATTTCGCCGTTCGTTGGCCGTATcctcg                                                                                               <  SRR12117898.1152724/121‑6 (MQ=60)
                                                         ctccacctggcagggcattcgcgccgctGAACAGCTGGAAAAAGAAGGCATTAACTGTAACCTGACGCTGCTGTTCTCCTTCGCTCAGGCTCGCGCTTGCGCCGAAGCGGGCGTCTTCCTGATTTCGCCGTTCGTTGGCCGTATCCTCGAC                                                                                             >  SRR12117898.228275/29‑151 (MQ=60)
                                                         ctccacctggcagggcattcgcgccgctGAACAGCTGGAAAAAGAAGGCATTAACTGTAACCTGACGCTGCTGTTCTCCTTCGCTCAGGCTCGCGCTTGCGCCGAAGCGGGCGTCTTCCTGATTTCGCCGTTCGTT                                                                                                            >  SRR12117898.402924/29‑136 (MQ=60)
                                                          TCTACCTGGCAGGGTATCCGTGCTGCAGAACAGCTGGAAAAAGAAGGCATCAACTGTAACCTGACCCTGCTGTTCTCCTTCGCTCAGGCTCGTGCTTGTGCGGAAGCGGGCGTGTTCCTGATCTCGCCGTTTGTTGGCCGTATTCTTGACT                                                                                            >  SRR12117898.772370/1‑151 (MQ=60)
                                                           CTACCTGGCAGGGTATCCGTGCTGCAGAACAGCTGGAAAAAGAAGGCATCAACTGTAACCTGACCCTGCTGTTCTCCTTCGCTCAGGCTCGTGCTTGTGCGGAAGCGGGCGTGTTCCTGATCTCGCCGTTTGTTGGCCGTATTCTTGACTG                                                                                           >  SRR12117898.698483/1‑151 (MQ=60)
                                                           CCACCTGGCAGGGCATTCGCGCCGCTGAACAGCTGGAAAAAGAAGGCATTAACTGTAACCTGACGCTGCTGTTCTCCTTCGCTCAGGCTCGCGCTTGCGCCGAAGCGGGCGTCTTCCTGATTTCGCCGTTCGTTGGCCGTATCCTCGACTG                                                                                           >  SRR12117898.309700/1‑151 (MQ=60)
                                                              CCTGGCAGGGCATTCGCGCCGCTGAACAGCTGGAAAAAGAAGGCATTAACTGTAACCTGACGCTGCTGTTCTCCTTCGCTCAGGCTCGCGCTTGCGCCGAAGCGGGCGTCTTCCTGATTTCGCCGTTCGTTGGCCGTATCCTCGACTGGT                                                                                         >  SRR12117898.826746/1‑150 (MQ=60)
                                                               CTGGCAGGGCATTCGCGCCGCTGAACAGCTGGAAAAAGAAGGCATTAACTGTAACCTGACGCTGCTGTTCTCCTTCGCTCAGGCTCGCGCTTGCGCCGAAGCGGGCGTCTTCCTGATTTCGCCGTTCGTTGGCCGTATCCTCGACTGGTAC                                                                                       >  SRR12117898.537307/1‑151 (MQ=60)
                                                                TGGCAGGGTATCCGTGCTGCAGAACAGCTGGAAAAAGAAGGCATCAACTGTAACCTGACCCTGCTGTTCTCCTTCGCTCAGGCTCGTGCTTGTGCGGAAGCGGGCGTGTTCCTGATCTCGCCGTTTGTTGGCCGTATTCTTGACTGGTAC                                                                                       >  SRR12117898.387076/1‑150 (MQ=60)
                                                                TGGCAGGGCATTCGCGCCGCTGAACAGCTGGAAAAAGAAGGCATTAACTGTAACCTGACGCTGCTGTTCTCCTTCGCTCAGGCTCGCGCTTGCGCCGAAGCGGGCGTCTTCCTGATTTCGCCGTTCGTTGGCCGTATCCTCGACTGGTAC                                                                                       >  SRR12117898.392435/1‑150 (MQ=60)
                                                                TGGCAGGGCATTCGCGCCGCTGAACAGCTGGAAAAAGAAGGCATTAACTGTAACCTGACGCTGCTGTTCTCCTTCGCTCAGGCTCGCGCTTGCGCCGAAGCGGGCGTCTTCCTGATTTCGCCGTTCGTTGGCCGTATCCTCGACTGGTAC                                                                                       >  SRR12117898.578093/1‑150 (MQ=60)
                                                                 ggcagggcattcgcgccgctGAACAGCTGGAAAAAGAAGGCATTAACTGTAACCTGACGCTGCTGTTCTCCTTCGCTCAGGCTCGCGCTTGCGCCGAAGCGGGCGTCTTCCTGATTTCGCCGTTCGTTGGCCGTATCCTCGACTGGTACA                                                                                      <  SRR12117898.436245/130‑1 (MQ=60)
                                                                  gcagggcattcgcgccgctGAACAGCTGGAAAAAGAAGGCATTAACTGTAACCTGACGCTGCTGTTCTCCTTCGCTCAGGCTCGCGCTTGCGCCGAAGCGGGCGTCTTCCTGATTTCGCCGTTCGTTGGCCGTATCCTCGACTGGTACAA                                                                                     >  SRR12117898.688360/20‑150 (MQ=60)
                                                                   cagggcattcgcgccgctGAACAGCTGGAAAAAGAAGGCATTAACTGTAACCTGACGCTGCTGTTCTCCTTCGCTCAGGCTCGCGCTTGCGCCGAAGCGGGCGTCTTCCTGATTTCGCCGTTCGTTGGCCGTATCCTCGACTGGTACAA                                                                                     >  SRR12117898.134797/19‑149 (MQ=60)
                                                                   CAGGGTATCCGTGCTGCAGAACAGCTGGAAAAAGAAGGCATCAACTGTAACCTGACCCTGCTGTTCTCCTTCGCTCAGGCTCGTGCTTGTGCGGAAGCGGGCGTGTTCCTGATCTCGCCGTTTGTTGGCCGTATTCTTGACTGGTACAAAG                                                                                   <  SRR12117898.847102/151‑1 (MQ=60)
                                                                   CAGGGTATCCGTGCTGCAGAACAGCTGGAAAAAGAAGGCATCAACTGTAACCTGACCCTGCTGTTCTCCTTCGCTCAGGCTCGTGCTTGTGCGGAAGCGGGCGTGTTCCTGATCTCGCCGTTTGTTGGCCGTATTCTTGACTGGTACAAAG                                                                                   <  SRR12117898.752714/151‑1 (MQ=60)
                                                                    agggcattcgcgccgctGAACAGCTGGAAAAAGAAGGCATTAACTGTAACCTGACGCTGCTGTTCTCCTTCGCTCAGGCTCGCGCTTGCGCCGAAGCGGGCGTCTTCCTGATTTCGCCGTTCGTTGGCCGTATCCTCGACTGGTACAAAGC                                                                                  >  SRR12117898.995468/18‑151 (MQ=60)
                                                                     GGGTATCCGTGCTGCAGAACAGCTGGAAAAAGAAGGCATCAACTGTAACCTGACCCTGCTGTTCTCCTTCGCTCAGGCTCGTGCTTGTGCGGAAGCGGGCGTGTTCCTGATCTCGCCGTTTGTTGGCCGTATTCTTGACTGGTACAAAGCG                                                                                 >  SRR12117898.723394/1‑151 (MQ=60)
                                                                     GGGTATCCGTGATGCAGAACAGCTGGAAAAAGAAGGCATCAACTGTAACCTGACCCTGCTGTTCTCCTTCGCTCAGGCTCGTGCTTGTGCGGAAGCGGGCGTGTTCCTGATCTCGCCGTTTGTTGGCCGTATTCTTGACTGGTACAAAGCG                                                                                 <  SRR12117898.1011874/151‑1 (MQ=60)
                                                                      ggcattcgcgccgctGAACAGCTGGAAAAAGAAGGCATTAACTGTAACCTGACGCTGCTGTTCTCCTTCGCTCAGGCTCGCGCTTGCGCCGAAGCGGGCGTCTTCCTGATTTCGCCGTTCGTTGGCCGTATCCTCGACTGGTACAAAGCC                                                                                 >  SRR12117898.431799/16‑150 (MQ=60)
                                                                        cattcgcgccgctGAACAGCTGGAAAAAGAAGGCATTAACTGTAACCTGACGCTGCTGTTCTCCTTCGCTCAGGCTCGCGCTTGCGCCGAAGCGGGCGTCTTCCTGATTTCGCCGTTCGTTGGCCGTATCCTCGACTGGTACAAAGCcaac                                                                              <  SRR12117898.203331/138‑5 (MQ=60)
                                                                        cattcgcgccgctGAACAGCTGGAAAAAGAAGGCATTAACTGTAACCTGACGCTGCTGTTCTCCTTCGCTCAGGCTCGCGCTTGCGCCGAAGCGGGCGTCTTCCTGATTTCGCCGTTCGTTGGCCGTATCCTCGACTGGTACAAAGCcaac                                                                              >  SRR12117898.452578/14‑147 (MQ=60)
                                                                            cgcgccgctGAACAGCTGGAAAAAGAAGGCATTAACTGTAACCTGACGCTGCTGTTCTCCTTCGCTCAGGCTCGCGCTTGCGCCGAAGCGGGCGTCTTCCTGATTTCGCCGTTCGTTGGCCGTATCCTCGACTGGTACAAAGCCAACACC                                                                           <  SRR12117898.666404/141‑1 (MQ=60)
                                                                            cgcgccgctGAACAGCTGGAAAAAGAAGGCATTAACTGTAACCTGACGCTGCTGTTCTCCTTCGCTCAGGCTCGCGCTTGCGCCGAAGCGGGCGTCTTCCTGATTTCGCCGTTCGTTGGCCGTATCCTCGACTGGTACAAAGCCAACACC                                                                           <  SRR12117898.983166/141‑1 (MQ=60)
                                                                             GTGCTGCAGAACAGCTGGAAAAAGAAGGCATCAACTGTAACCTGACCCTGCTGTTCTCCTTCGCTCAGGCTCGTGCTTGTGCGGAAGCGGGCGTGTTCCTGATCTCGCCGTTTGTTGGCCGTATTCTTGACTGGTACAAAGCGAATACCG                                                                          >  SRR12117898.255526/1‑150 (MQ=60)
                                                                               GCTGCAGAACAGCTGGAAAAAGAAGGCATCAACTGTAACCTGACCCTGCTGTTCTCCTTCGCTCAGGCTCGTGCTTGTGCGGAAGCGGGCGTGTTCCTGATCTCGCCGTTTGTTGGCCGTATTCTTGACTGGTACAAAGCGAATACCGAT                                                                        >  SRR12117898.557926/1‑150 (MQ=60)
                                                                               GCCGCTGAACAGCTGGAAAAAGAAGGCATTAACTGTAACCTGACGCTGCTGTTCTCCTTCGCTCAGGCTCGCGCTTGCGCCGAAGCGGGCGTCTTCCTGATTTCGCCGTTCGTTGGCCGTATCCTCGACTGGTACAAAGCcaacaccgac                                                                        >  SRR12117898.745286/1‑140 (MQ=60)
                                                                                  GCTGAACAGCTGGAAAAAGAAGGCATTAACTGTAACCTGACGCTGCTGTTCTCCTTCGCTCAGGCTCGCGCTTGCGCCGAAGCGGGCGTCTTCCTGATTTCGCCGTTCGTTGGCCGTATCCTCGACTGGTACAAAGCCAACACCGACAAGA                                                                    <  SRR12117898.489742/151‑1 (MQ=60)
                                                                                  GCAGAACAGCTGGAAAAAGAAGGCATCAACTGTAACCTGACCCTGCTGTTCTCCTTCGCTCAGGCTCGTGCTTGTGCGGAAGCGGGCGTGTTCCTGATCTCGCCGTTTGTTGGCCGTATTCTTGACTGGTACAAAGCGAATACCGATAAGA                                                                    <  SRR12117898.1093939/151‑1 (MQ=60)
                                                                                    TGAACAGCTGGAAAAAGAAGGCATTAACTGTAACCTGACGCTGCTGTTCTCCTTCGCTCAGGCTCGCGCTTGCGCCGAAGCGGGCGTCTTCCTGATTTCGCCGTTCGTTGGCCGTATCCTCGACTGGTACAAAGCCAACACCGACAAGAA                                                                   >  SRR12117898.654828/1‑150 (MQ=60)
                                                                                    TGAACAGCTGGAAAAAGAAGGCATTAACTGTAACCTGACGCTGCTGTTCTCCTTCGCTCAGGCTCGCGCTTGCGCCGAAGCGGGCGTCTTCCTGATTTCGCCGTTCGTTGGCCGTATCCTCGACTGGTACAAAGCCAACACCGACAAGAA                                                                   >  SRR12117898.70223/1‑150 (MQ=60)
                                                                                     GAACAGCTGGAAAAAGAAGGCATTAACTGTAACCTGACGCTGCTGTTCTCCTTCGCTCAGGCTCGCGCTTGCGCCGAAGCGGGCGTCTTCCTGATTTCGCCGTTCGTTGGCCGTATCCTCGACTGGTACAAAGCCAACACCGACAAGAAAG                                                                 <  SRR12117898.52773/151‑1 (MQ=60)
                                                                                       ACAGCTGGAAAAAGAAGGCATTAACTGTAACCTGACGCTGCTGTTCTCCTTCGCTCAGGCTCGCGCTTGCGCCGAAGCGGGCGTCTTCCTGATTTCGCCGTTCGTTGGCCGTATCCTCGACTGGTACAAAGCCAACACCGACAAGAAAG                                                                 >  SRR12117898.277383/1‑149 (MQ=60)
                                                                                        CAGCTGGAAAAAGAAGGCATTAACTGTAACCTGACGCTGCTGTTCTCCTTCGCTCAGGCTCGCGCTTGCGCCGAAGCGGGCGTCTTCCTGATTTCGCCGTTCGTTGGCCGTATCCTCGACTGGTACAAAGCCAACACCGACAAGAAAGAGT                                                              >  SRR12117898.333112/1‑151 (MQ=60)
                                                                                         AGCTGGAAAAAGAAGGCATCAACTGTAACCTGACCCTGCTGTTCTCCTTCGCTCAGGCTCGTGCTTGTGCGGAAGCGGGCGTGTTCCTGATCTCGCCGTTTGTTGGCCGTATTCTTGACTGGTACAAAGCGAATACCGATAAGAAAGAGT                                                              >  SRR12117898.949266/1‑150 (MQ=60)
                                                                                          GCTGGAAAAAGTAGGCATCAACTGTAACCTGACCCTGCTGTTCTCCTTCGCTCAGGCTCGTGCTTGTGCGGAAGCGGGCGTGTTCCTGATCTCGCCGTTTGTTGGCCGTATTCTTGACTGGTACAAAGCGAATACCGATAAGAAAGAGTAC                                                            <  SRR12117898.249106/151‑1 (MQ=60)
                                                                                           CTGGAAAAAGAAGGCATTAACTGTAACCTGACGCTGCTGTTCTCCTTCGCTCAGGCTCGCGCTTGCGCCGAAGCGGGCGTCTTCCTGATTTCGCCGTTCGTTGGCCGTATCCTCGACTGGTACAAAGCCAACACCGACAAGAAAGAGTACG                                                           >  SRR12117898.925005/1‑151 (MQ=60)
                                                                                                 AAAGAAGGCATTAACTGTAACCTGACGCTGCTGTTCTCCTTCGCTCAGGCTCGCGCTTGCGCCGAAGCGGGCGTCTTCCTGATTTCGCCGTTCGTTGGCCGTATCCTCGACTGGTACAAAGCCAACACCGACAAGAAAGAGTACGCACCGG                                                     <  SRR12117898.746005/151‑1 (MQ=60)
                                                                                                      AGGCATTAACTGTAACCTGACGCTGCTGTTCTCCTTCGCTCAGGCTCGCGCTTGCGCCGAAGCGGGCGTCTTCCTGATTTCGCCGTTCGTTGGCCGTATCCTCGACTGGTACAAAGCCAACACCGACAAGAAAGAGTACGCACCGGCAGA                                                 >  SRR12117898.1077967/1‑150 (MQ=60)
                                                                                                       GGCATTAACTGTAACCTGACGCTGCTGTTCTCCTTCGCTCAGGCTCGCGCTTGCGCCGAAGCGGGCGTCTTCCTGATTTCGCCGTTCGTTGGCCGTATCCTCGACTGGTACAAAGCCAACACCGACAAGAAAGAGTACGCACCGGCAGAAG                                               <  SRR12117898.552022/151‑1 (MQ=60)
                                                                                                         CATTAACTGTAACCTGACGCTGCTGTTCTCCTTCGCTCAGGCTCGCGCTTGCGCCGAAGCGGGCGTCTTCCTGATTTCGCCGTTCGTTGGCCGTATCCTCGACTGGTACAAAGCCAACACCGACAAGAAAGAGTACGCACCGGCAGAAGAT                                             <  SRR12117898.808660/151‑1 (MQ=60)
                                                                                                          ATTAACTGTAACCTGACGCTGCTGTTCTCCTTCGCTCAGGCTCGCGCTTGCGCCGAAGCGGGCGTCTTCCTGATTTCGCCGTTCGTTGGCCGTATCCTCGACTGGTACAAAGCCAACACCGACAAGAAAGAGTACGCACCGGCAGAAGATC                                            <  SRR12117898.355784/151‑1 (MQ=60)
                                                                                                          ATTAACTGTAACCTGACGCTGCTGTTCTCCTTCGCTCAGGCTCGCGCTTGCGCCGAAGCGGGCGTCTTCCTGATTTCGCCGTTCGTTGGCCGTATCCTCGACTGGTACAAAGCCAACACCGACAAGAAAGAGTACGCACCGGCAGA                                                 >  SRR12117898.1072244/1‑146 (MQ=60)
                                                                                                            CAACTGTAACCTGACCCTGCTGTTCTCCTTCGCTCAGGCTCGTGCTTGTGCGGAAGCGGGCGTGTTCCTGATCTCGCCGTTTGTTGGCCGTATTCTTGACTGGTACAAAGCGAATACCGATAAGAAAGAGTACGCTCCGGCAGAAGATCC                                           <  SRR12117898.661824/150‑1 (MQ=60)
                                                                                                             AACTGTAACCTGACGCTGCTGTTCTCCTTCGCTCAGGCTCGCGCTTGCGCCGAAGCGGGCGTCTTCCTGATTTCGCCGTTCGTTGGCCGTATCCTCGACTGGTACAAAGCCAACACCGACAAGAAAGAGTACGCACCGGCAGAAGATCCG                                          <  SRR12117898.1166604/150‑1 (MQ=60)
                                                                                                             AACTGTAACCTGACGCTGCTGTTCTCCTTCGCTCAGGCTCGCGCTTGCGCCGAAGCGGGCGTCTTCCTGATTTCGCCGTTCGTTGGCCGTATCCTCGACTGGTACAAAGCCAACACCGACAAGAAAGAGTACGCACCGGCAGAAGATCCG                                          <  SRR12117898.171996/150‑1 (MQ=60)
                                                                                                             AACTGTAACCTGACCCTGCTGTTCTCCTTCGCTCAGGCTCGTGCTTGTGCGGAAGCGGGCGTGTTCCTGATCTCGCCGTTTGTTGGCCGTATTCTTGACTGGTACAAAGCGAATACCGATAAGAAAGAGTACGCTCCGGCAGAAGATCCGG                                         <  SRR12117898.537834/151‑1 (MQ=60)
                                                                                                              ACTGTAACCTGACGCTGCTGTTCTCCTTCGCTCAGGCTCGCGCTTGCGCCGAAGCGGGCGTCTTCCTGATTTCGCCGTTCGTTGGCCGTATCCTCGACTGGTACAAAGCCAACACCGACAAGAAAGAGTACGCACCGGCAGAAGATCCGGG                                        >  SRR12117898.254229/1‑151 (MQ=60)
                                                                                                               CTGTAACCTGACGCTGCTGTTCTCCTTCGCTCAGGCTCGCGCTTGCGCCGAAGCGGGCGTCTTCCTGATTTCGCCGTTCGTTGGCCGTATCCTCGACTGGTACAAAGCCAACACCGACAAGAAAGAGTACGCACCGGCAGAAGATCCGGGC                                       >  SRR12117898.767006/1‑151 (MQ=60)
                                                                                                               CTGTAACCTGACCCTGCTGTTCTCCTTCGCTCAGGCTCGTGCTTGTGCGGAAGCGGGCGTGTTCCTGATCTCGCCGTTTGTTGGCCGTATTCTTGACTGGTACAAAGCGAATACCGATAAGAAAGAGTACGCTCCGGCAGAAGATCCGGGC                                       >  SRR12117898.934720/1‑151 (MQ=60)
                                                                                                                  TAACCTGACGCTGCTGTTCTCCTTCGCTCAGGCTCGCGCTTGCGCCGAAGCGGGCGTCTTCCTGATTTCGCCGTTCGTTGGCCGTATCCTCGACTGGTACAAAGCCAACACCGACAAGAAAGAGTACGCACCGGCAGAAGATCCGGGCGTG                                    >  SRR12117898.711856/1‑151 (MQ=60)
                                                                                                                   AACCTGACGCTGCTGTTCTCCTTCGCTCAGGCTCGCGCTTGCGCCGAAGCGGGCGTCTTCCTGATTTCGCCGTTCGTTGGCCGTATCCTCGACTGGTACAAAGCCAACACCGACAAGAAAGAGTACGCACCGGCAGAAGATCCGGGCGTG                                    <  SRR12117898.81632/150‑1 (MQ=60)
                                                                                                                   AACCTGACGCTGCTGTTCTCCTTCGCTCAGGCTCGCGCTTGCGCCGAAGCGGGCGTCTTCCTGATTTCGCCGTTCGTTGGCCGTATCCTCGACTGGTACAAAGCCAACACCGACAAGAAAGAGTACGCACCGGCAGAAGATCCGGGCGTG                                    <  SRR12117898.254202/150‑1 (MQ=60)
                                                                                                                   AACCTGACGCTGCTGTTCTCCTTCGCTCAGGCTCGCGCTTGCGCCGAAGCGGGCGTCTTCCTGATTTCGCCGTTCGTTGGCCGTATCCTCGACTGGTACAAAGCCAACACCGACAAGAAAGAGTACGCACCGGCAGAAGATCCGGGCGTG                                    <  SRR12117898.591460/150‑1 (MQ=60)
                                                                                                                   AACCTGACCCTGCTGTTCTCCTTCGCTCAGGCTCGTGCTTGTGCGGAAGCGGGCGTGTTCCTGATCTCGCCGTTTGTTGGCCGTATTCTTGACTGGTACAAAGCGAATACCGATAAGAAAGAGTACGCTCCGGCAGAAGATCCGGGCGTG                                    <  SRR12117898.465981/150‑1 (MQ=60)
                                                                                                                     CCTGACGCTGCTGTTCTCCTTCGCTCAGGCTCGCGCTTGCGCCGAAGCGGGCGTCTTCCTGATTTCGCCGTTCGTTGGCCGTATCCTCGACTGGTACAAAGCCAACACCGACAAGAAAGAGTACGCACCGGCAGAAGATCCGGGCGTGGTT                                 >  SRR12117898.260101/1‑151 (MQ=60)
                                                                                                                     CCTGACGCTGCTGTTCTCCTTCGCTCAGGCTCGCGCTTGCGCCGAAGCGGGCGTCTTCCTGATTTCGCCGTTCGTTGGCCGTATCCTCGACTGGTACAAAGCCAACACCGACAAGAAAGAGTACGCACCGGCAGAAGATCCGGGCGTGGTT                                 >  SRR12117898.1178554/1‑151 (MQ=60)
                                                                                                                     CCTGACCCTGCTGTTCTCCTTCGCTCAGGCTCGTGCTTGTGCGGAAGCGGGCGTGTTCCTGATCTCGCCGTTTGTTGGCCGTATTCTTGACTGGTACAAAGCGAATACCGATAAGAAAGAGTACGCTCCGGCAGAAGATCCGGGCGTGGTT                                 <  SRR12117898.909615/151‑1 (MQ=60)
                                                                                                                       TGACGCTGCTGTTCTCCTTCGCTCAGGCTCGCGCTTGCGCCGAAGCGGGCGTCTTCCTGATTTCGCCGTTCGTTGGCCGTATCCTCGACTGGTACAAAGCCAACACCGACAAGAAAGAGTACGCACCGGCAGAAGATCCGGGCGTGGTTTC                               >  SRR12117898.1183407/1‑151 (MQ=60)
                                                                                                                       TGACCCTGCTGTTCTCCTTCGCTCAGGCTCGTGCTTGTGCGGAAGCGGGCGTGTTCCTGATCTCGCCGTTTGTTGGCCGTATTCTTGACTGGTACAAAGCGAATACCGATAAGAAAGAGTACGCTCCGGCAGAAGATCCGGGCGTGGTTTC                               >  SRR12117898.881938/1‑151 (MQ=60)
                                                                                                                        GACGCTGCTGTTCTCCTTCGCTCAGGCTCGCGCTTGCGCCGAAGCGGGCGTCTTCCTGATTTCGCCGTTCGTTGGCCGTATCCTCGACTGGTACAAAGCCAACACCGACAAGAAAGAGTACGCACCGGCAGAAGATCCGGGCGTGGTTTCC                              >  SRR12117898.253484/1‑151 (MQ=60)
                                                                                                                         ACGCTGCTGTTCTCCTTCGCTCAGGCTCGCGCTTGCGCCGAAGCGGGCGTCTTCCTGATTTCGCCGTTCGTTGGCCGTATCCTCGACTGGTACAAAGCCAACACCGACAAGAAAGAGTACGCACCGGCAGAAGATCCGGGCGTGGTTTCCG                             <  SRR12117898.351964/151‑1 (MQ=60)
                                                                                                                              GCTGTTCTCCTTCGCTCAGGCTCGCGCTTGCGCCGAAGCGGGCGTCTTCCTGATTTCGCCGTTCGTTGGCCGTATCCTCGACTGGTACAAAGCCAACACCGACAAGAAAGAGTACGCACCGGCAGAAGATCCGGGCGTGGTTTCcgtcagc                        <  SRR12117898.1020411/151‑8 (MQ=60)
                                                                                                                               CTGTTCTCCTTCGCTCAGGCTCGCGCTTGCGCCGAAGCGGGCGTCTTCCTGATTTCGCCGTTCGTTGGCCGTATCCTCGACTGGTACAAAGCCAACACCGACAAGAAAGAGTACGCACCGGCAGAAGATCCGGGCGTGGTTTCcgtcagcg                       >  SRR12117898.1153949/1‑143 (MQ=60)
                                                                                                                                   TCTCCTTCGCTCAGGCTCGCGCTTGCGCCGAAGCGGGCGTCTTCCTGATTTCGCCGTTCGTTGGCCGTATCCTCGACTGGTACAAAGCCAACACCGACAAGAAAGAGTACGCACCGGCAGAAGATCCGGGCGTGGTTTCcgtcagcgaaat                   >  SRR12117898.1158113/1‑139 (MQ=60)
                                                                                                                                   TCTCCTTCGCTCAGGCTCGCGCTTGCGCCGAAGCGGGCGTCTTCCTGATTTCGCCGTTCGTTGGCCGTATCCTCGACTGGTACAAAGCCAACACCGACAAGAAAGAGTACGCACCGGCAGAAGATCCGGGCGTGGTTTCcgtcagcgaaa                    <  SRR12117898.429293/150‑12 (MQ=60)
                                                                                                                                   TCTCCTTCGCTCAGGCTCGCGCTTGCGCCGAAGCGGGCGTCTTCCTGATTTCGCCGTTCGTTGGCCGTATCCTCGACTGGTACAAAGCCAACACCGACAAGAAAGAGTACGCACCGGCAGAAGATCCGGGCGTGGTTTCcgtcagcgaaa                    <  SRR12117898.849991/150‑12 (MQ=60)
                                                                                                                                     TCCTTCGCTCAGGCTCGCGCTTGCGCCGAAGCGGGCGTCTTCCTGATTTCGCCGTTCGTTGGCCGTATCCTCGACTGGTACAAAGCCAACACCGACAAGAAAGAGTACGCACCGGCAGAAGATCCGGGCGTGGTTTCcgtcagcgaaatct                 >  SRR12117898.963124/1‑137 (MQ=60)
                                                                                                                                      CCTTCGCTCAGGCTCGCGCTTGCGCCGAAGCGGGCGTCTTCCTGATTTCGCCGTTCGTTGGCCGTATCCTCGACTGGTACAAAGCCAACACCGACAAGAAAGAGTACGCACCGGCAGAAGATCCGGGCGTGGTTTCcgtcagcgaaatct                 >  SRR12117898.222324/1‑136 (MQ=60)
                                                                                                                                       CTTCGCTCAGGCTCGCGCTTGCGCCGAAGCGGGCGTCTTCCTGATTTCGCCGTTCGTTGGCCGTATCCTCGACTGGTACAAAGCCAACACCGACAAGAAAGAGTACGCACCGGCAGAAGATCCGGGCGTGGTTTCcgtcagcgaaatctac               >  SRR12117898.60896/1‑135 (MQ=60)
                                                                                                                                         TCGCTCAGGCTCGTGCTTGTGCGGAAGCGGGCGTGTTCCTGATCTCGCCGTTTGTTGGCCGTATTCTTGACTGGTACAAAGCGAATACCGATAAGAAAGAGTACGCTCCGGCAGAAGATCCGGGCGTGGTTTCTGTATCTGAAATCTACC              <  SRR12117898.87180/150‑1 (MQ=60)
                                                                                                                                         TCGCTCAGGCTCGTGCTTGTGCGGAAGCGGGCGTGTTCCTGATCTCGCCGTTTGTTGGCCGTATTCTTGACTGGTACAAAGCGAATACCGATAAGAAAGAGTACGCTCCGGCAGAAGATCCGGGCGTGGTTTCTGTATCTGAAATCTAC               <  SRR12117898.599437/149‑1 (MQ=60)
                                                                                                                                          CGCTCAGGCTCGCGCTTGCGCCGAAGCGGGCGTCTTCCTGATTTCGCCGTTCGTTGGCCGTATCCTCGACTGGTACAAAGCCAACACCGACAAGAAAGAGTACGCACCGGCAGAAGATCCGGGCGTGGTTTCcgtcagcgaaatctacga             <  SRR12117898.395851/150‑19 (MQ=60)
                                                                                                                                           GCTCAGGCTCGCGCTTGCGCCGAAGCGGGCGTGTTCCTGATTTCGCCGTTCGTTGGCCGTATCCTCGACTGGTACAAAGCCAACACCGACAAGAAAGAGTACGCACCGGCAGAAGATCCGGGCGTGGTTTCcgtcagcgaaatctacgagt           >  SRR12117898.162092/1‑131 (MQ=60)
                                                                                                                                            CTCAGGCTCGTGCTTGTGCGGAAGCGGGCGTGTTCCTGATCTCGCCGTTTGTTGGCCGTATTCTTGACTGGTACAAAGCGAATACCGATAAGAAAGAATACGCTCCGGCAGAAGATCCGGGCGTGGTTTCTGTATCTGAAATCTACCAGT           >  SRR12117898.256677/1‑150 (MQ=60)
                                                                                                                                             TCAGGCTCGTGCTTGTGCGGAAGCGGGCGTGTTCCTGATCTCGCCGTTTGTTGGCCGTATTCTTGACTGGTACAAAGCGAATACCGATAAGAAAGAGTACGCTCCGGCAGAAGATCCGGGCGTGGTTTCTGTATCTGAA                     <  SRR12117898.637820/139‑1 (MQ=60)
                                                                                                                                             TCAGGCTCGCGCTTGCGCCGAAGCGGGCGTCTTCCTGATTTCGCCGTTCGTTGGCCGTATCCTCGACTGGTACAAAGCCAACACCGACAAGAAAGAGTACGCACCGGCAGAAGATCCGGGCGTGGTTTCcgtcagcgaaatctacgagtat         >  SRR12117898.842954/1‑129 (MQ=60)
                                                                                                                                              CAGGCTCGTGCTTGTGCGGAAGCGGGCGTGTTCCTGATCTCGCCGTTTGTTGGCCGTATTCTTGACTGGTACAAAGCGAATACCGATAAGAAAGAGTACGCTCCGGCAGAAGATCCGGGCGTGGTTTCTGTATCTGAAATCTACCAGTAC         <  SRR12117898.140208/150‑1 (MQ=60)
                                                                                                                                               AGGCTCGCGCTTGCGCCGAAGCGGGCGTCTTCCTGATTTCGCCGTTCGTTGGCCGTATCCTCGACTGGTACAAAGCCAACACCGACAAGAAAGAGTACGCACCGGCAGAAGATCCGGGCGTGGTTTCcgtcagcgaaatctacgagtatt        >  SRR12117898.788988/1‑127 (MQ=60)
                                                                                                                                                 GCTCGCGCTTGCGCCGAAGCGGGCGTCTTCCTGATTTCGCCGTTCGTTGGCCGTATCCTCGACTGGTACAAAGCCAACACCGACAAGAAAGAGTACGCACCGGCAGAAGATCCGGGCGTGGTTTCcgtcagcgaaatctacgagtattaca     <  SRR12117898.125877/151‑27 (MQ=60)
                                                                                                                                                 GCTCGCGCTTGCGCCGAAGCGGGCGTCTTCCTGATTTCGCCGTTCGTTGGCCGTATCCTCGACTGGTACAAAGCCAACACCGACAAGAAAGAGTACGCACCGGCAGAAGATCCGGGCGTGGTTTCcgtcagcgaaatctacgagtattaca     <  SRR12117898.596735/151‑27 (MQ=60)
                                                                                                                                                   TCGCGCTTGCGCCGAAGCGGGCGTCTTCCTGATTTCGCCGTTCGTTGGCCGTATCCTCGACTGGTACAAAGCCAACACCGACAAGAAAGAGTACGCACCGGCAGAAGATCCGGGCGTGGTTTCcgtcagcgaaatctacgagtattaca     >  SRR12117898.519797/1‑123 (MQ=60)
                                                                                                                                                    CGTGCTTGTGCGGAAGCGGGCGTGTTCCTGATCTCGCCGTTTGTTGGCCGTATTCTTGACTGGTACAAAGCGAATACCGATAAGAAAGAGTACGCTCCGGCAGAAGATCCGGGCGTGGTTTCTGTATCTGAAATCTTCCAGTACTACAAAG  <  SRR12117898.925242/151‑1 (MQ=60)
                                                                                                                                                    CGTGCTTGTGCGGAAGCGGGCGTGTTCCTGATCTCGCCGTTTGTTGGCCGTATTCTTGACTGGTACAAAGCGAATACCGATAAGAAAGAGTACGCTCCGGCAGAAGATCCGGGCGTGGTTTCTGTATCTGAAATCTACCAGTACTACAAAG  <  SRR12117898.118193/151‑1 (MQ=60)
                                                                                                                                                    CGTGCTTGTGCGGAAGCGGGCGTGTTCCTGATCTCGCCGTTTGTTGGCCGTATTCTTGACTGGTACAAAGCGAATACCGATAAGAAAGAGTACGCTCCGGCAGAAGATCCGGGCGTGGTTTCTGTATCTGAAATCTACCAGTACTACAAA   <  SRR12117898.637139/150‑1 (MQ=60)
                                                                                                                                                    CGCGCTTGCGCCGAAGCGGGCGTCTTCCTGATTTCGCCGTTCGTTGGCCGTATCCTCGACTGGTACAAAGCCAACACCGACAAGAAAGAGTACGCACCGGCAGAAGATCCGGGCGTGGTTTCcgtcagcgaaatctacgagtattacaagc  <  SRR12117898.225083/151‑30 (MQ=60)
                                                                                                                                                      |                                                                                                                                                    
GATCAAACTCTACAACGATGCTGGTATTAGCAACGATCGTATTCTGATCAAACTGGCTTCTACCTGGCAGGGTATCCGTGCTGCAGAACAGCTGGAAAAAGAAGGCATCAACTGTAACCTGACCCTGCTGTTCTCCTTCGCTCAGGCTCGTGCTTGTGCGGAAGCGGGCGTGTTCCTGATCTCGCCGTTTGTTGGCCGTATTCTTGACTGGTACAAAGCGAATACCGATAAGAAAGAGTACGCTCCGGCAGAAGATCCGGGCGTGGTTTCTGTATCTGAAATCTACCAGTACTACAAAG  >  CP009273/8582‑8880

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑