Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F16 I0 R1 235 70.4 2387208 96.6% 2306042 149.2

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 9,068 T→C 60.0% T277T (ACT→ACC)  talB → transaldolase B

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092739,0680TC60.0% 108.8 / 196.4 155T277T (ACT→ACC) talBtransaldolase B
Reads supporting (aligned to +/- strand):  ref base T (28/34);  new base C (36/57);  total (64/91)
Fisher's exact test for biased strand distribution p-value = 5.06e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.36e-09

Only 100 of 157 total aligned reads displayed.

GTAACATCGGCGAAATTCTGGAACTGGCAGGCTGCGACCGTCTGACCATCGCACCGGCACTGCTGAAAGAGCTGGCGGAGAGCGAAGGGGCTATCGAACGTAAACTGTCTTACACCGGCGAAGTGAAAGCGCGTCCGGCGCGTATCACTGAGTCCGAGTTCCTGTGGCAGCACAACCAGGATCCAATGGCAGTAGATAAACTGGCGGAAGGTATCCGTAAGTTTGCTATTGACCAGGAAAAACTGGAAAAAATGATCGGCGATCTGCTGTAATCATTCTTAGCGTGACCGGGAAGTCG  >  CP009273/8920‑9217
                                                                                                                                                    |                                                                                                                                                     
  aaCATCGGCGAAATTCTGGAACTGGCAGGCTGCGACCGTCTGACCATCGCACCGGCACTGCTGAAAGAGCTGGCGGAGAGCGAAGGGGCTATCGAACGTAAACTGTCTTACACCGGCGAAGTGAAAGCGCGTCCGGCGCGTATCACTGAg                                                                                                                                                    >  2:407673/1‑150 (MQ=255)
     gttggCGAAATTCTGGAACTGGCTGGCTGTGACCGCCTGACCATCGCCCCGGCGCTGCTGAAAGAGCTGTCCGAAAGCGAAGGCGCCGTTGAGCGTAAGCTGGTATATACCGGTGAAGTGAAAGCGCGTCCGGAGCGTATTACCGAATct                                                                                                                                                 >  2:640525/4‑149 (MQ=255)
       tggCGAAATTCTGGAACTGGCTGGCTGTGACCGCCTGACCATCGCCCCGGCGCTGCTGAAAGAGCTGTCCGAAAGCGAAGGCGCCGTTGAGCGCAAGCTGGCATATACCGGTGAAGTGAAAGCGCGTCCGGAGCGTATTACCGAATct                                                                                                                                                 >  2:420504/2‑147 (MQ=255)
         gCGAAATTCTGGAACTGGCTGGCTGTGACCGCCTGACCATCGCCCCGGCGCTGCTGAAAGAGCTGTCCGAAAGCGAAGGCGCCGTTGAGCGTAAGCTGGTATATACCGGTGAAGTGAAAGCGCGTCCGGAGCGTATTACCGAATCTGAg                                                                                                                                              <  1:1117900/149‑1 (MQ=255)
         gCGAAATTCTGGAACTGGCAGGCTGCGACCGTCTGACCATCGCACCGGCACTGCTGAAAGAGCTGGCGGAGAGCGAAGGGGCTATCGAACGTAAACTGTCTTACACCGGCGAAGTGAAAGCGCGTCCGGCGCGTATCACTGAGTCCGAGt                                                                                                                                             <  1:624767/150‑1 (MQ=255)
          cGAAATTCTGGAACTGGCAGGCTGCGACCGTCTGACCATCGCACCGGCACTGCTGAAAGAGCTGGCGGAGAGCGAAGGGGCTATCGAACGTAAACTGTCTTACACCGGCGAAGTGAAAGCGCGTCCGGCGCGTATCACTGAGTCCGAGtt                                                                                                                                            <  2:878377/150‑1 (MQ=255)
           gAAATTCTGGAACTGGCTGGCTGTGACCGCCTGACCATCGCCCCGGCGCTGCTGAAAGAGCTGTCCGAAAGCGAAGGCGCCGTTGAGCGTAAGCTGGTATATACCGGTGAAGTGAAAGCGCGTCCGGAGCGTATTACCGAATCTGAGTTc                                                                                                                                           >  1:830186/1‑150 (MQ=255)
           gAAATTCTGGAACTGGCAGGCTGCGACCGTCTGACCATCGCACCGGCACTGCTGAAAGAGCTGGCGGAGAGCGAAGGGGCTATCGAACGTAAACTGTCTTACACCGGCGAAGTGAAAGCGCGTCCGGCGCGTATCACTGAGTCCGAGTTc                                                                                                                                           <  2:337745/150‑1 (MQ=255)
            aaaTTCTGGAACTGGCTGGCTGTGACCGCCTGACCATCGCCCCGGCGCTGCTGAAAGAGCTGTCCGAAAGCGAAGGCGCCGTTGAGCGTAAGCTGGTATATACCGGTGAAGTGAAAGCGCGTCCGGAGCGTATTACCGAATCTGAGTTcc                                                                                                                                          <  1:552482/150‑1 (MQ=255)
            aaaTTCTGGAACTGGCTGGCTGTGACCGCCTGACCATCGCCCCGGCGCTGCTGAAAGAGCTGTCCGAAAGCGAAGGCGCCGTTGAGCGTAAGCTGGTATATACCGGTGAAGTGAAAGCGCGTCCGGAGCGTATTACCGAATCTGAGTTcc                                                                                                                                          <  1:611081/150‑1 (MQ=255)
            aaaTTCTGGAACTGGCTGGCTGTGACCGCCTGACCATCGCCCCGGCGCTGCTGAAAGAGCTGTCCGAAAGCGAAGGCGCCGTTGAGCGTAAGCTGGTATATACCGGTGAAGTGAAAGCGCGTCCGGAGCGTATTACCGAATCTGAGTTcc                                                                                                                                          <  1:460038/150‑1 (MQ=255)
               ttCTGGAACTGGCAGGCTGCGACCGTCTGACCATCGCACCGGCACTGCTGAAAGAGCTGGCGGAGAGCGAAGGGGCTATCGAACGTAAACTGTCTTACACCGGCGAAGTGAAAGCGCGTCCGGCGCGTATCACTGAGTCCGAGTTCCtgt                                                                                                                                       >  1:46191/1‑150 (MQ=255)
                       ctggctggCTGTGACCGCCTGACCATCGCCCCGGCGCTGCTGAAAGAGCTGTCCGAAAGCGAAGGCGCCGTTGAGCGTAAGCTGGTATATACCGGTGAAGTGAAAGCGCGTCCGGAGCGTATTACCGAATCTGAGTTCCTGTGgcagca                                                                                                                                >  2:946813/1‑149 (MQ=255)
                          gCAGGCTGCGACCGTCTGACCATCGCACCGGCACTGCTGAAAGAGCTGGCGGAGAGCGAAGGGGCTATCGAACGTAAACTGTCTTACACCGGCGAAGTGAAAGCGCGTCCGGCGCGTATCACTGAGTCCGAGTTCCTGTGGCAGCACaa                                                                                                                             <  2:925497/149‑1 (MQ=255)
                                   gACCGCCTGACCATCGCCCCGGCGCTGCTGAAAGAGCTGTCCGAAAGCGAAGGCGCCGTTGAGCGTAAGCTGGTATATACCGGTGAAGTGAAAGCGCGTCCGGAGCGTATTACCGAATCTGAGTTCCTGTGGCAGCATAACCAGGATcc                                                                                                                    >  1:552802/1‑149 (MQ=255)
                                       gccTGACCATCGCCCCGGCGCTGCTGAAAGAGCTGTCCGAAAGCGAAGGCGCCGTTGAGCGTAAGCTGGTATATACCGGTGAAGTGAAAGCGCGTCCGGAGCGTATTACCGAATCTGAGTTCCTGTGGCAGCATAACCAGGATCCGATgg                                                                                                               >  2:379293/3‑150 (MQ=255)
                                        ccTGACCATCGCCCCGGCGCTGCTGAAAGAGCTGTCCGAAAGCGAAGGCGCCGTTGAGCGTAAGCTGGTATATACCGGTGAAGTGAAAGCGCGTCCGGAGCGTATTACCGAATCTGAGTTCCTGTGGCAGCATAACCAGGATCCGATGGc                                                                                                              <  2:7601/149‑1 (MQ=255)
                                            aCCATCGCACCGGCACTGCTGAAAGAGCTGGCGGAGAGCGAAGGGGCTATCGAACGTAAACTGTCTTACACCGGCGAAGTGAAAGCGCGTCCGGCGCGTATCACTGAGTCCGAGTTCCTGTGGCAGCACAACCAGGATCCAATGGCAGTa                                                                                                          <  2:206543/150‑1 (MQ=255)
                                              cATCGCACCGGCACTGCTGAAAGAGCTGGCGGAGAGCGAAGGGGCTATCGAACGTAAACTGTCTTACACCGGCGAAGTGAAAGCGCGTCCGGCGCGTATCACTGAGTCCGAGTTCCTGTGGCAGCACAACCAGGATCCAATGGCAGTAGa                                                                                                        <  1:841011/150‑1 (MQ=255)
                                                    aCCGGCACTGCTGAAAGAGCTGGCGGAGAGCGAAGGGGCTATCGAACGTAAACTGTCTTACACCGGCGAAGTGAAAGCGCGTCCGGCGCGTATCACTGAGTCCGAGTTCCTGTGGCAGCACAACCAGGATCCAATGGCAGTAGATAAACt                                                                                                  >  2:69323/1‑150 (MQ=255)
                                                          aCTGCTGAAAGAGCTGGCGGAGAGCGAAGGGGCTATCGAACGTAAACTGTCTTACACCGGCGAAGTGAAAGCGCGTCCGGCGCGTATCACTGAGTCCGAGTTCCTGTGGCAGCACAACCAGGATCCAATGGCAGTAGATAAACTGGCgg                                                                                             >  1:1060469/1‑149 (MQ=255)
                                                           ctgctgAAAGAGCTGTCCGAAAGCGAAGGCGCCGTTGAGCGTAAGCTGGTATATACCGGTGAAGTGAAAGCGCGTCCGGAGCGTATTACCGAATCTGAGTTCCTGTGGCAGCATAACCAGGATCCGATGGCGGTAGACAAACTGGCgg                                                                                             >  2:1099073/1‑148 (MQ=255)
                                                           ctgctgAAAGAGCTGGCGGAGAGCGAAGGGGCTATCGAACGTAAACTGTCTTACACCGGCGAAGTGAAAGCGCGTCCGGCGCGTATCACTGAGTCCGAGTTCCTGTGGCAGCACAACCAGGATCCAATGGCAGTAGATAAACTGGCgg                                                                                             >  2:497110/1‑148 (MQ=255)
                                                                 aaaGAGCTGTCCGAAAGCGAAGGCGCCGTTGAGCGTAAGCTGGTATATACCGGTGAAGTGAAAGCGCGTCCGGAGCGTATTACCGAATCTGAGTTCCTGTGGCAGCATAACCAGGATCCGATGGCGGTAGACAAACTGGCGGAAGGTAt                                                                                      <  2:440491/149‑1 (MQ=255)
                                                                  aaGAGCTGTCCGAAAGCGAAGGCGCCGTTGAGCGTAAGCTGGTATATACCGGTGAAGTGAAAGCGCGTCCGGAGCGTATTACCGAATCTGAGTTCCTGTGGCAGCATAACCAGGATCCGATGGCGGTAGACAAACTGGCGGAAGGTATcc                                                                                    <  2:565520/150‑1 (MQ=255)
                                                                   agagCTGGCGGAGAGCGAAGGGGCTATCGAACGTAAACTGTCTTACACCGGCGAAGTGAAAGCGCGTCCGGCGCGTATCACTGAGTCCGAGTTCCTGTGGCAGCACAACCAGGATCCAATGGCAGTAGATAAACTGGCGGAAGGTATCCg                                                                                   <  2:500970/150‑1 (MQ=255)
                                                                   agagCTGGCGGAGAGCGAAGGGGCTATCGAACGTAAACTGTCTTACACCGGCGAAGTGAAAGCGCGTCCGGCGCGTATCACTGAGTCCGAGTTCCTGTGGCAGCACAACCAGGATCCAATGGCAGTAGATAAACTGGCGGAAGGTATCCg                                                                                   <  2:107499/150‑1 (MQ=255)
                                                                    gagCTGTCCGAAAGCGAAGGCGCCGTTGAGCGTAAGCTGGTATATACCGGTGAAGTGAAAGCGCGTCCGGAGCGTATTACCGAATCTGAGTTCCTGTGGCAGCATAACCAGGATCCGATGGCGGTAGACAAACTGGCGGAAGGTATCCGt                                                                                  <  2:617690/150‑1 (MQ=255)
                                                                    gagCTGTCCGAAAGCGAAGGCGCCGTTGAGCGTAAGCTGGTATATACCGGTGAAGTGAAAGCGCGTCCGGAGCGTATTACCGAATCTGAGTTCCTGTGGCAGCATAACCAGGATCCGATGGCGGTAGACAAACTGGCGGAAGGTATCCGt                                                                                  <  1:296084/150‑1 (MQ=255)
                                                                     agCTGTCCGAAAGCGAAGGCGCCGTTGAGCGTAAGCTGGTATATACCGGTGAAGTGAAAGCGCGTCCGGAGCGTATTACCGAATCTGAGTTCCTGTGGCAGCATAACCAGGATCCGATGGCGGTAGACAAACTGGCGGAAGGTATCCGTa                                                                                 <  2:822847/150‑1 (MQ=255)
                                                                     agCTGGCGGAGAGCGAAGGGGCTATCGAACGTAAACTGTCTTACACCGGCGAAGTGAAAGCGCGTCCGGCGCGTATCACTGAGTCCGAGTTCCTGTGGCAGCACAACCAGGATCCAATGGCAGTAGATAAACTGGCGGAAGGTATCCGt                                                                                  >  1:771723/1‑149 (MQ=255)
                                                                      gCTGTCCGAAAGCGAAGGCGCCGTTGAGCGTAAGCTGGTATATACCGGTGAAGTGAAAGCGCGTCCGGAGCGTATTACCGAATCTGAGTTCCTGTGGCAGCATAACCAGGATCCGATGGCGGTAGACAAACTGGCGGAAGGTATCCGTaa                                                                                <  2:304233/150‑1 (MQ=255)
                                                                      gCTGTCCGAAAGCGAAGGCGCCGTTGAGCGTAAGCTGGTATATACCGGTGAAGTGAAAGCGCGTCCGGAGCGTATTACCGAATCTGAGTTCCTGTGGCAGCATAACCAGGATCCGATGGCGGTAGACAAACTGGCGGAAGGTATCCGTaa                                                                                <  2:825504/150‑1 (MQ=255)
                                                                      gCTGTCCGAAAGCGAAGGCGCCGTTGAGCGTAAGCTGGTATATACCGGTGAAGTGAAAGCGCGTCCGGAGCGTATTACCGAATCTGAGTTCCTGTGGCAGCATAACCAGGATCCGATGGCGGTAGACAAACTGGCGGAAGGTATCCGTaa                                                                                <  2:89771/150‑1 (MQ=255)
                                                                         gtccgaaagCGAAGGCGCCGTTGAGCGTAAGCTGGTATATACCGGTGAAGTGAAAGCGCGTCCGGAGCGTATTACCGAATCTGAGTTCCTGTGGCAGCATAACCAGGATCCGATGGCGGTAGACAAACTGGCGGAAGGTATCCGTAAGtt                                                                             >  1:305181/8‑150 (MQ=255)
                                                                          tccgaaagCGAAGGCGCCGTTGAGCGTAAGCTGGTATATACCGGTGAAGTGAAAGCGCGTCCGGAGCGTATTACCGAATCTGAGTTCCTGTGGCAGCATAACCAGGATCCGATGGCGGTAGACAAACTGGCGGAAGGTATCCGTAAGttt                                                                            <  2:604448/144‑1 (MQ=255)
                                                                          tccgaaagCGAAGGCGCCGTTGAGCGTAAGCTGGTATATACCGGTGAAGTGAAAGCGCGTCCGGAGCGTATTACCGAATCTGAGTTCCTGTGGCAGCATAACCAGGATCCGATGGCGGTAGACAAACTGGCGGAAGGTATCCGTAAGttt                                                                            <  2:1095317/144‑1 (MQ=255)
                                                                          tccgaaagCGAAGGCGCCGTTGAGCGTAAGCTGGTATATACCGGTGAAGTGAAAGCGCGTCCGGAGCGTATTACCGAATCTGAGTTCCTGTGGCAGCATAACCAGGATCCGATGGCGGTAGACAAACTGGCGGAAGGTATCCGTAAGtt                                                                             <  1:192822/143‑1 (MQ=255)
                                                                          tccgaaagCGAAGGCGCCGTTGAGCGTAAGCTGGTATATACCGGTGAAGTGAAAGCGCGTCCGGAGCGTATTACCGAATCTGAGTTCCTGTGGCAGCATAACCAGGATCCGATGGCGGTAGACAAACTGGCGGAAGGTATCCGTAAGtt                                                                             <  1:292146/143‑1 (MQ=255)
                                                                          tccgaaagCGAAGGCGCCGTTGAGCGTAAGCTGGTATATACCGGTGAAGTGAAAGCGCGTCCGGAGCGTATTACCGAATCTGAGTTCCTGTGGCAGCATAACCAGGATCCGATGGCGGTAGACAAACTGGCGGAAGGTATCCGTAAGtt                                                                             <  1:122294/143‑1 (MQ=255)
                                                                          gCGGAGAGCGAAGGGGCTATCGAACGTAAACTGTCTTACACCGGCGAAGTGAAAGCGCGTCCGGCGCGTATCACTGAGTCCGAGTTCCTGTGGCAGCACAACCAGGATCCAATGGCAGTAGATAAACTGGCGGAAGGTATCCGTAAGttt                                                                            >  1:120707/1‑150 (MQ=255)
                                                                             gagaGCGAAGGGGCTATCGAACGTAAACTGTCTTACACCGGCGAAGTGAAAGCGCGTCCGGCGCGTATCACTGAGTCCGAGTTCCTGTGGCAGCACAACCAGGATCCAATGGCAGTAGATAAACTGGCGGAAGGTATCCGTAAGTTTGCt                                                                         <  1:677555/150‑1 (MQ=255)
                                                                               aagCGAAGGCGCCGTTGAGCGTAAGCTGGTATATACCGGTGAAGTGAAAGCGCGTCCGGAGCGTATTACCGAATCTGAGTTCCTGTGGCAGCATAACCAGGATCCGATGGCGGTAGACAAACTGGCGGAAGGTATCCGTAAGTTTGCGAt                                                                       <  1:1096495/149‑1 (MQ=255)
                                                                                agCGAAGGCGCCGTTGAGCGTAAGCTGGTATATACCGGTGAAGTGAAAGCGCGTCCGGAGCGTATTACCGAATCTGAGTTCCTGTGGCAGCATAACCAGGATCCGATGGCGGTAGACAAACTGGCGGAAGGTATCCGTAAGTTTGCGAtc                                                                      <  1:516100/150‑2 (MQ=255)
                                                                                agCGAAGGCGCCGTTGAGCGTAAGCTGGTATATACCGGTGAAGTGAAAGCGCGTCCGGAGCGTATTACCGAATCTGAGTTCCTGTGGCAGCATAACCAGGATCCGATGGCGGTAGACAAACTGGCGGAAGGTATCCGTAAGTTTGCGAtc                                                                      >  1:201946/1‑149 (MQ=255)
                                                                                agCGAAGGCGCCGTTGAGCGTAAGCTGGTATATACCGGTGAAGTGAAAGCGCGTCCGGAGCGTATTACCGAATCTGAGTTCCTGTGGCAGCATAACCAGGATCCGATGGCGGTAGACAAACTGGCGGAAGGTATCCGTAAGTTTGCGAtc                                                                      <  2:552802/150‑2 (MQ=255)
                                                                                agCGAAGGCGCCGTTGAGCGTAAGCTGGTATATACCGGTGAAGTGAAAGCGCGTCCGGAGCGTATTACCGAATCTGAGTTCCTGTGGCAGCATAACCAGGATCCGATGGCGGTAGACAAACTGGCGGAAGGTATCCGTAAGTTTGCGAtc                                                                      <  2:680495/150‑2 (MQ=255)
                                                                                 gCGAAGGCGCCGTTGAGCGTAAGCTGGTATATACCGGTGAAGTGAAAGCGCGTCCGGAGCGTATTACCGAATCTGAGTTCCTGTGGCAGCATAACCAGGATCCGATGGCGGTAGACAAACTGGCGGAAGGTATCCGTAAGTTTGCGAtcg                                                                     <  2:1040356/150‑3 (MQ=255)
                                                                                 gCGAAGGCGCCGTTGAGCGTAAGCTGGTATATACCGGTGAAGTGAAAGCGCGTCCGGAGCGTATTACCGAATCTGAGTTCCTGTGGCAGCATAACCAGGATCCGATGGCGGTAGACAAACTGGCGGAAGGTATCCGTAAGTTTGCGAtc                                                                      >  1:173339/1‑148 (MQ=255)
                                                                                  cGAAGGGGCTATCGAACGTAAACTGTCTTACACCGGCGAAGTGAAAGCGCGTCCGGCGCGTATCACTGAGTCCGAGTTCCTGTGGCAGCACAACCAGGATCCAATGGCAGTAGATAAACTGGCGGAAGGTATCCGTAAGTTTGCTATTGa                                                                    <  2:518964/150‑1 (MQ=255)
                                                                                  cGAAGGGGCTATCGAACGTAAACTGTCTTACACCGGCGAAGTGAAAGCGCGTCCGGCGCGTATCACTGAGTCCGAGTTCCTGTGGCAGCACAACCAGGATCCAATGGCAGTAGATAAACTGGCGGAAGGTATCCGTAAGTTTGCTATTGa                                                                    >  1:355758/1‑150 (MQ=255)
                                                                                  cGAAGGGGCTATCGAACGTAAACTGTCTTACACCGGCGAAGTGAAAGCGCGTCCGGCGCGTATCACTGAGTCCGAGTTCCTGTGGCAGCACAACCAGGATCCAATGGCAGTAGATAAACTGGCGGAAGGTATCCGTAAGTTTGCTATTGa                                                                    >  2:24321/1‑150 (MQ=255)
                                                                                      ggggCTATCGAACGTAAACTGTCTTACACCGGCGAAGTGAAAGCGCGTCCGGCGCGTATCACTGAGTCCGAGTTCCTGTGGCAGCACAACCAGGATCCAATGGCAGTAGATAAACTGGCGGAAGGTATCCGTAAGTTTGCTATTGACCAg                                                                >  2:1134194/1‑150 (MQ=255)
                                                                                         gCTATCGAACGTAAACTGTCTTACACCGGCGAAGTGAAAGCGCGTCCGGCGCGTATCACTGAGTCCGAGTTCCTGTGGCAGCACAACCAGGATCCAATGGCAGTAGATAAACTGGCGGAAGGTATCCGTAAGTTTGCTATTGACCAGGa                                                              >  1:1042522/1‑149 (MQ=255)
                                                                                           tATCGAACGTAAACTGTCTTACACCGGCGAAGTGAAAGCGCGTCCGGCGCGTATCACTGAGTCCGAGTTCCTGTGGCAGCACAACCAGGATCCAATGGCAGTAGATAAACTGGCGGAAGGTATCCGTAAGTTTGCTATTGACCAGGaaa                                                            >  1:391232/1‑149 (MQ=255)
                                                                                           tATCGAACGTAAACTGTCTTACACCGGCGAAGTGAAAGCGCGTCCGGCGCGTATCACTGAGTCCGAGTTCCTGTGGCAGCACAACCAGGATCCAATGGCAGTAGATAAACTGGCGGAAGGTATCCGTAAGTTTGCTATTGACCAGGaaa                                                            >  2:250373/1‑149 (MQ=255)
                                                                                            aTCGAACGTAAACTGTCTTACACCGGCGAAGTGAAAGCGCGTCCGGCGCGTATCACTGAGTCCGAGTTCCTGTGGCAGCACAACCAGGATCCAATGGCAGTAGATAAACTGGCGGAAGGTATCCGTAAGTTTGCTATTGACCAGGaaaaa                                                          >  1:758653/1‑150 (MQ=255)
                                                                                             tCGAACGTAAACTGTCTTACACCGGCGAAGTGAAAGCGCGTCCGGCGCGTATCACTGAGTCCGAGTTCCTGTGGCAGCACAACCAGGATCCAATGGCAGTAGATAAACTGGCGGAAGGTATCCGTAAGTTTGCTATTGACCAGGAAAAAc                                                         <  1:696100/150‑1 (MQ=255)
                                                                                               gAACGTAAACTGTCTTACACCGGCGAAGTGAAAGCGCGTCCGGCGCGTATCACTGAGTCCGAGTTCCTGTGGCAGCACAACCAGGATCCAATGGCAGTAGATAAACTGGCGGAAGGTATCCGTAAGTTTGCTATTGACCAGGAAAAACTg                                                       >  1:980262/1‑150 (MQ=255)
                                                                                                      aaCTGTCTTACACCGGCGAAGTGAAAGCGCGTCCGGCGCGTATCACTGAGTCCGAGTTCCTGTGGCAGCACAACCAGGATCCAATGGCAGTAGATAAACTGGCGGAAGGTATCCGTAAGTTTGCTATTGACCAGGAAAAACTGGaaaaa                                                 <  1:1134194/149‑1 (MQ=255)
                                                                                                         tGTCTTACACCGGCGAAGTGAAAGCGCGTCCGGCGCGTATCACTGAGTCCGAGTTCCTGTGGCAGCACAACCAGGATCCAATGGCAGTAGATAAACTGGCGGAAGGTATCCGTAAGTTTGCTATTGACCAGGAAAAACTGGAAAAAATGa                                             >  1:168140/1‑150 (MQ=255)
                                                                                                           gtatatacCGGTGAAGTGAAAGCGCGTCCGGAGCGTATTACCGAATCTGAGTTCCTGTGGCAGCATAACCAGGATCCGATGGCGGTAGACAAACTGGCGGAAGGTATCCGTAAGTTTGCGATCGACCAGGAAAAACTGGAAAAAATGa                                             <  2:748836/142‑1 (MQ=255)
                                                                                                           gtatatacCGGTGAAGTGAAAGCGCGTCCGGAGCGTATTACCGAATCTGAGTTCCTGTGGCAGCATAACCAGGATCCGATGGCGGTAGACAAACTGGCGGAAGGTATCCGTAAGTTTGCGATCGACCAGGAAAAACTGGAAAAAATGa                                             <  1:65398/142‑1 (MQ=255)
                                                                                                           gtatatacCGGTGAAGTGAAAGCGCGTCCGGAGCGTATTACCGAATCTGAGTTCCTGTGGCAGCATAACCAGGATCCGATGGCGGTAGACAAACTGGCGGAAGGTATCCGTAAGTTTGCGATCGACCAGGAAAAACTGGAAAAAATGATc                                           >  2:645393/7‑150 (MQ=255)
                                                                                                           gtatatacCGGTGAAGTGAAAGCGCGTCCGGAGCGTATTACCGAATCTGAGTTCCTGTGGCAGCATAACCAGGATCCGATGGCGGTAGACAAACTGGCGGAAGGTATCCGTAAGTTTGCGATCGACCAGGAAAAACTGGAAAAAATGATc                                           >  2:1145094/7‑150 (MQ=255)
                                                                                                           gtatatacCGGTGAAGTGAAAGCGCGTCCGGAGCGTATTACCGAATCTGAGTTCCTGTGGCAGCATAACCAGGATCCGATGGCGGTAGACAAACTGGCGGAAGGTATCCGTAAGTTTGCGATCGACCAGGAAAAACTGGAAAAAATGATc                                           <  2:1119199/144‑1 (MQ=255)
                                                                                                              tatacCGGTGAAGTGAAAGCGCGTCCGGAGCGTATTACCGAATCTGAGTTCCTGTGGCAGCATAACCAGGATCCGATGGCGGTAGACAAACTGGCGGAAGGTATCCGTAAGTTTGCGATCGACCAGGAAAAACTGGAAAAAATGATCgac                                        <  1:977653/147‑3 (MQ=255)
                                                                                                              tatacCGGTGAAGTGAAAGCGCGTCCGGAGCGTATTACCGAATCTGAGTTCCTGTGGCAGCATAACCAGGATCCGATGGCGGTAGACAAACTGGCGGAAGGTATCCGTAAGTTTGCGATCGACCAGGAAAAACTGGAAAAAATGATCgac                                        >  1:1025510/4‑148 (MQ=255)
                                                                                                               acacCGGCGAAGTGAAAGCGCGTCCGGCGCGTATCACTGAGTCCGAGTTCCTGTGGCAGCACAACCAGGATCCAATGGCAGTAGATAAACTGGCGGAAGGTATCCGTAAGTTTGCTATTGACCAGGAAAAACTGGAAAAAATGATCGGc                                        <  1:900858/149‑1 (MQ=255)
                                                                                                                tacCGGTGAAGTGAAAGCGCGTCCGGAGCGTATTACCGAATCTGAGTTCCTGTGGCAGCATAACCAGGATCCGATGGCGGTAGACAAACTGGCGGAAGGTATCCGTAAGTTTGCGATCGACCAGGAAAAACTGGAAAAAATGATCGACg                                       <  1:384967/148‑1 (MQ=255)
                                                                                                                tacCGGTGAAGTGAAAGCGCGTCCGGAGCGTATTACCGAATCTGAGTTCCTGTGGCAGCATAACCAGGATCCGATGGCGGTAGACAAACTGGCGGAAGGTATCCGTAAGTTTGCGATCGACCAGGAAAAACTGGAAAAAATGATCGACg                                       <  1:727675/148‑1 (MQ=255)
                                                                                                                cacCGGCGAAGTGAAAGCGCGTCCGGCGCGTATCACTGAGTCCGAGTTCCTGTGGCAGCACAACCAGGATCCAATGGCAGTAGATAAACTGGCGGAAGGTATCCGTAAGTTTGCTATTGACCAGGAAAAACTGGAAAAAATGATCGGCg                                       <  1:1064288/149‑1 (MQ=255)
                                                                                                                  ccGGCGAAGTGAAAGCGCGTCCGGCGCGTATCACTGAGTCCGAGTTCCTGTGGCAGCACAACCAGGATCCAATGGCAGTAGATAAACTGGCGGAAGGTATCCGTAAGTTTGCTATTGACCAGGAAAAACTGGAAAAAATGATCGGCGATc                                    >  2:522803/1‑150 (MQ=255)
                                                                                                                   cGGTGAAGTGAAAGCGCGTCCGGAGCGTATTACCGAATCTGAGTTCCTGTGGCAGCATAACCAGGATCCGATGGCGGTAGACAAACTGGCGGAAGGTATCCGTAAGTTTGCGATCGACCAGGAAAAACTGGAAAAAATGATCGACGAAct                                   >  2:1024700/1‑150 (MQ=255)
                                                                                                                   cGGCGAAGTGAAAGCGCGTCCGGCGCGTATCACTGGGTCCGAGTTCCTGTGGCAGCACAACCAGGATCCAATGGCAGTAGATAAACTGGCGGAAGGTATCCGTAAGTTTGCTATTGACCAGGAAAAACTGGAAAAAATGATCGGCGATct                                   >  2:424514/1‑150 (MQ=255)
                                                                                                                   cGGCGAAGTGAAAGCGCGTCCGGCGCGTATCACTGAGTCCGAGTTCCTGTGGCAGCACAACCAGGATCCAATGGCAGTAGATAAACTGGCGGAAGGTATCCGTAAGTTTGCTATTGACCAGGAAAAACTGGAAAAAATGATCGGCGATct                                   <  1:407673/150‑1 (MQ=255)
                                                                                                                    ggtgAAGTGAAAGCGCGTCCGGAGCGTATTACCGAATCTGAGTTCCTGTGGCAGCATAACCAGGATCCGATGGCGGTAGACAAACTGGCGGAAGGTATCCGTAAGTTTGCGATCGACCAGGAAAAACTGGAAAAAATGATCGACGAActg                                  >  2:326476/4‑150 (MQ=255)
                                                                                                                    ggtgAAGTGAAAGCGCGTCCGGAGCGTATTACCGAATCTGAGTTCCTGTGGCAGCATAACCAGGATCCGATGGCGGTAGACAAACTGGCGGAAGGTATCCGTAAGTTTGCGATCGACCAGGAAAAACTGGAAAAAATGATCGACGAActg                                  <  2:723468/147‑1 (MQ=255)
                                                                                                                    ggCGAAGTGAAAGCGCGTCCGGCGCGTATCACTGAGTCCGAGTTCCTGTGGCAGCACAACCAGGATCCAATGGCAGTAGATAAACTGGCGGAAGGTATCCGTAAGTTTGCTATTGACCAGGAAAAACTGGAAAAAATGATCGGCGATctg                                  <  2:796720/150‑1 (MQ=255)
                                                                                                                      tgAAGTGAAAGCGCGTCCGGAGCGTATTACCGAATCTGAGTTCCTGTGGCAGCATAACCAGGATCCGATGGCGGTAGACAAACTGGCGGAAGGTATCCGTAAGTTTGCGATCGACCAGGAAAAACTGGAAAAAATGATCGACGAActgct                                <  1:946813/149‑1 (MQ=255)
                                                                                                                      tgAAGTGAAAGCGCGTCCGGAGCGTATTACCGAATCTGAGTTCCTGTGGCAGCATAACCAGGATCCGATGGCGGTAGACAAACTGGCGGAAGGTATCCGTAAGTTTGCGATCGACCAGGAAAAACTGGAAAAAATGATCGACGAActgct                                <  1:668224/149‑1 (MQ=255)
                                                                                                                       gAAGTGAAAGCGCGTCCGGCGCGTATCACTGAGTCCGAGTTCCTGTGGCAGCACAACCAGGATCCAATGGCAGTAGATAAACTGGCGGAAGGTATCCGTAAGTTTGCTATTGACCAGGAAAAACTGGAAAAAATGATCGGCGATctgctg                               >  2:1013747/1‑150 (MQ=255)
                                                                                                                       gAAGTGAAAGCGCGTCCGGAGCGTATTACCGAATCTGAGTTCCTGTGGCAGCATAACCAGGATCCGATGGCGGTAGACAAACTGGCGGAAGGTATCCGTAAGTTTGCGAt                                                                       >  1:670427/1‑110 (MQ=255)
                                                                                                                       gAAGTGAAAGCGCGTCCGGAGCGTATTACCGAATCTGAGTTCCTGTGGCAGCATAACCAGGATCCGATGGCGGTAGACAAACTGGCGGAAGGTATCCGTAAGTTTGCGATCGACCAGGAAAAACTGGAAAAAATGATCGACGAActgctg                               <  2:864323/150‑1 (MQ=255)
                                                                                                                        aaGTGAAAGCGCGTCCGGAGCGTATTACCGAATCTGAGTTCCTGTGGCAGCATAACCAGGATCCGATGGCGGTAGACAAACTGGCGGAAGGTATCCGTAAGTTTGCGAtc                                                                      <  2:670427/110‑2 (MQ=255)
                                                                                                                        aaGTGAAAGCGCGTCCGGAGCGTATTACCGAATCTGAGTTCCTGTGGCAGCATAACCAGGATCCGATGGCGGTAGACAAACTGGCGGAAGGTATCCGTAAGTTTGCGATCGACCAGGAAAAACTGGAAAAAATGATCGACGAActgctg                               >  1:598130/1‑149 (MQ=255)
                                                                                                                        aaGTGAAAGCGCGTCCGGAGCGTATTACCGAATCTGAGTTCCTGTGGCAGCATAACCAGGATCCGATGGCGGTAGACAAACTGGCGGAAGGTATCCGTAAGTTTGCGATCGACCAGGAAAAACTGGAAAAAATGATCGACGAActgctg                               >  2:408503/1‑149 (MQ=255)
                                                                                                                         aGTGAAAGCGCGTCCGGAGCGTATTACCGAATCTGAGTTCCTGTGGCAGCATAACCAGGATCCGATGGCGGTAGACAAACTGGCGGAAGGTATCCGTAAGTTTGCGATCGACCAGGAAAAACTGGAAAAAATGATCGACGAACTGCTGTa                             <  1:642624/150‑1 (MQ=255)
                                                                                                                          gTGAAAGCGCGTCCGGAGCGTATTACCGAATCTGAGTTCCTGTGGCAGCATAACCAGGATCCGATGGCGGTAGACAAACTGGCGGAAGGTATCCGTAAGTTTGCGATCGACCAGGAAAAACTGGAAAAAATGATcgacgaa                                     >  1:1169086/1‑140 (MQ=255)
                                                                                                                           tGAAAGCGCGTCCGGAGCGTATTACCGAATCTGAGTTCCTGTGGCAGCATAACCAGGATCCGATGGCGGTAGACAAACTGGCGGAAGGTATCCGTAAGTTTGCGATCGACCAGGAAAAACTGGAAAAAATGATcgacgaac                                    <  2:1169086/141‑3 (MQ=255)
                                                                                                                           tGAAAGCGCGTCCGGAGCGTATTACCGAATCTGAGTTCCTGTGGCAGCATAACCAGGATCCGATGGCGGTAGACAAACTGGCGGAAGGTATCCGTAAGTTTGCGATCGACCAGGAAAAACTGGAAAAAATGATCGACGAACTGCTGt                              >  1:1120585/1‑147 (MQ=255)
                                                                                                                           tGAAAGCGCGTCCGGAGCGTATTACCGAATCTGAGTTCCTGTGGCAGCATAACCAGGATCCGATGGCGGTAGACAAACTGGCGGAAGGTATCCGTAAGTTTGCGATCGACCAGGAAAAACTGGAAAAAATGATCGACGAACTGCTGt                              >  1:737485/1‑147 (MQ=255)
                                                                                                                            gAAAGCGCGTCCGGAGCGTATTACCGAATCTGAGTTCCTGTGGCAGCATAACCAGGATCCGATGGCGGTAGACAAACTGGCGGAAGGTATCCGTAAGTTTGCGATCGACCAGGAAAAACTGGAATAAATGATCGACGAACTGCTGTaa                            >  1:661029/1‑148 (MQ=255)
                                                                                                                             aaaGCGCGTCCGGCGCGTATCACTGAGTCCGAGTTCCTGTGGCAGCACAACCAGGATCCAATGGCAGTAGATAAACTGGCGGAAGGTATCCGTAAGTTTGCTATTGACCAGGAAAAACTGGAAAAAATGATCGGCGATCTGCTGTAATc                          <  2:460422/149‑1 (MQ=255)
                                                                                                                             aaaGCGCGTCCGGAGCGTATTACCGAATCTGAGTTCCTGTGGCAGCATAACCAGGATCCGATGGCGGTAGACAAACTGGCGGAAGGTATCCGTAAGTTTGCGATCGACCAGGAAAAACTGGAAAAAATGATCGACGAACTGCTGTaaga                          <  1:849142/149‑3 (MQ=255)
                                                                                                                              aaGCGCGTCCGGAGCGTATTACCGAATCTGAGTTCCTGTGGCAGCATAACCAGGATCCGATGGCGGTAGACAAACTGGCGGAAGGTATCCGTAAGTTTGCGATCGACCAGGAAAAACTGGAAAAAATGATCGACGAACTGCTGTaagag                         >  2:572042/1‑146 (MQ=255)
                                                                                                                               aGCGCGTCCGGAGCGTATTACCGAATCTGAGTTCCTGTGGCAGCATAACCAGGATCCGATGGCGGTAGACAAACTGGCGGAAGGTATCCGTAAGTTTGCGATCGACCAGGAAAAACTGGAAAAAATGATCGACGAACTGCTGTaagagaa                       >  2:1190756/1‑145 (MQ=255)
                                                                                                                                       cGGCGCGTATCACTGAGTCCGAGTTCCTGTGGCAGCACAACCAGGATCCAATGGCAGTAGATAAACTGGCGGAAGGTATCCGTAAGTTTGCTATTGACCAGGAAAAACTGGAAAAAATGATCGGCGATCTGCTGTAATCATTCTTAGCGt               <  2:461997/150‑1 (MQ=255)
                                                                                                                                                  aCTGAGTCCGAGTTCCTGTGGCAGCACAACCAGGATCCAATGGCAGTAGATAAACTGGCGGAAGGTATCCGTAAGTTTGCTATTGACCAGGAAAAACTGGAAAAAATGATCGGCGATCTGCTGTAATCATTCTTAGCGTGACCGGGAAg     <  2:695746/149‑1 (MQ=255)
                                                                                                                                                    tGAGTCCGAGTTCCTGTGGCAGCACAACCAGGATCCAATGGCAGTAGATAAACTGGCGGAAGGTATCCGTAAGTTTGCTATTGACCAGGAAAAACTGGAAAAAATGATCGGCGATCTGCTGTAATCATTCTTAGCGTGACCGGGAAGTCg  >  2:356791/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                                     
GTAACATCGGCGAAATTCTGGAACTGGCAGGCTGCGACCGTCTGACCATCGCACCGGCACTGCTGAAAGAGCTGGCGGAGAGCGAAGGGGCTATCGAACGTAAACTGTCTTACACCGGCGAAGTGAAAGCGCGTCCGGCGCGTATCACTGAGTCCGAGTTCCTGTGGCAGCACAACCAGGATCCAATGGCAGTAGATAAACTGGCGGAAGGTATCCGTAAGTTTGCTATTGACCAGGAAAAACTGGAAAAAATGATCGGCGATCTGCTGTAATCATTCTTAGCGTGACCGGGAAGTCG  >  CP009273/8920‑9217

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GTAACATCGGCGAAATTCTGGAACTGGCAGGCTGCGACCGTCTGACCATCGCACCGGCACTGCTGAAAGAGCTGGCGGAGAGCGAAGGGGCTATCGAACGTAAACTGTCTTACACCGGCGAAGTGAAAGCGCGTCCGGCGCGTATCACTGAGTCCGAGTTCCTGTGGCAGCACAACCAGGATCCAATGGCAGTAGATAAACTGGCGGAAGGTATCCGTAAGTTTGCTATTGACCAGGAAAAACTGGAAAAAATGATCGGCGATCTGCTGTAATCATTCTTAGCGTGACCGGGAAGTC  >  CP009273/8920‑9216
                                                                                                                                                    |                                                                                                                                                    
GTAACATCGGCGAAATTCTGGAACTGGCAGGCTGCGACCGTCTGACCATCGCACCGGCACTGCTGAAAGAGCTGGCGGAGAGCGAAGGGGCTATCGAACGTAAACTGTCTTACACCGGCGAAGTGAAAGCGCGTCCGGCGCGTATCACTG                                                                                                                                                     >  SRR12117898.520992/1‑150 (MQ=60)
        GGCGAAATTCTGGAACTGGCAGGCTGCGACCGTCTGACCATCGCACCGGCACTGCTGAAAGAGCTGGCGGAGAGCGAAGGGGCTATCGAACGTAAACTGTCTTACACCGGCGAAGTGAAAGCGCGTCCGGCGCGTATCACTGAGTCCGAGT                                                                                                                                            <  SRR12117898.627208/151‑1 (MQ=60)
               TTCTGGAACTGGCAGGCTGCGACCGTCTGACCATCGCACCGGCACTGCTGAAAGAGCTGGCGGAGAGCGAAGGGGCTATCGAACGTAAACTGTCTTACACCGGCGAAGTGAAAGCGCGTCCGGCGCGTATCACTGAGTCCGAGTTCCTGTG                                                                                                                                     >  SRR12117898.46376/1‑151 (MQ=60)
                      ACTGGCTGGCTGTGACCGCCTGACCATCGCCCCGGCGCTGCTGAAAGAGCTGTCCGAAAGCGAAGGCGCCGTTGAGCGTAAGCTGGTATATACCGGTGAAGTGAAAGCGCGTCCGGAGCGTATTACCGAATCTGAGTTCCTGTGGCAGCAT                                                                                                                              <  SRR12117898.472439/151‑1 (MQ=12)
                      ACTGGCTGGCTGTGACCGCCTGACCATCGCCCCGGCGCTGCTGAAAGAGCTGTCCGAAAGCGAAGGCGCCGTTGAGCGTAAGCTGGTATATACCGGTGAAGTGAAAGCGCGTCCGGAGCGTATTACCGAATCTGAGTTCCTGTGGCAGCAT                                                                                                                              <  SRR12117898.652832/151‑1 (MQ=12)
                          GCAGGCTGCGACCGTCTGACCATCGCACCGGCACTGCTGAAAGAGCTGGCGGAGAGCGAAGGGGCTATCGAACGTAAACTGTCTTACACCGGCGAAGTGAAAGCGCGTCCGGCGCGTATCACTGAGTCCGAGTTCCTGTGGCAGCACAACC                                                                                                                          <  SRR12117898.120866/151‑1 (MQ=60)
                           CAGGCTGCGACCGTCTGACCATCGCACCGGCACTGCTGAAAGAGCTGGCGGAGAGCGAAGGGGCTATCGAACGTAAACTGTCTTACACCGGCGAAGTGAAAGCGCGTCCGGCGCGTATCACTGAGTCCGAGTTCCTGTGGCAGCACAACCA                                                                                                                         <  SRR12117898.294492/151‑1 (MQ=60)
                           CAGGCTGCGACCGTCTGACCATCGCACCGGCACTGCTGAAAGAGCTGGCGGAGAGCGAAGGGGCTATCGAACGTAAACTGTCTTACACCGGCGAAGTGAAAGCGCGTCCGGCGCGTATCACTGAGTCCGAGTTCCTGTGGCAGCACAACC                                                                                                                          >  SRR12117898.462226/1‑150 (MQ=60)
                             ggctgtgaccgcctgaccatcgccccggcgctgctgaaagagctgtccgaaagcgaaggcgccgttgagcgtaagctggtatatACCGGTGAAGTGAAAGCGCGTCCGGAGCGTATTACCGAATCTGAGTTCCTGTGGCAGCATAACCAGG                                                                                                                       >  SRR12117898.867839/85‑151 (MQ=26)
                             GGCTGTGACCGCCTGACCATCGCCCCGGCGCTGCTGAAAGAGCTGTCCGAAAGCGAAGGCGCCGTTGAGCGTAAGCTGGTATATACCGGTGAAGTGAAAGCGCGTCCGGAGCGTATTACCGAATCTGAGTTCCTGTGGCAGCATAACCAGG                                                                                                                       <  SRR12117898.409603/151‑1 (MQ=14)
                             GGCTGTGACCGCCTGACCATCGCCCCGGCGCTGCTGAAAGAGCTGTCCGAAAGCGAAGGCGCCGTTGAGCGTAAGCTGGTATATACCGGTGAAGTGAAAGCGCGTCCGGAGCGTATTACCGAATCTGAGTTCCTGTGGCAGCATAACCAG                                                                                                                        <  SRR12117898.460772/150‑1 (MQ=13)
                                 gtgaccgcctgaccatcgccccggcgctgctgaaagagctgtccgaaagcgaaggcgccgttgagcgtaagctggtatatACCGGTGAAGTGAAAGCGCGTCCGGAGCGTATTACCGAATCTGAGTTCCTGTGGCAGCATAACCAGGATCC                                                                                                                   >  SRR12117898.726348/81‑151 (MQ=33)
                                 gtgaccgcctgaccatcgccccggcgctgctgaaagagctgtccgaaagcgaaggcgccgttgagcgtaagctggtatatACCGGTGAAGTGAAAGCGCGTCCGGAGCGTATTACCGAATCTGAGTTCCTGTGGCAGCATAACCAGGAT                                                                                                                     <  SRR12117898.756155/69‑1 (MQ=30)
                                  tgaccgcctgaccatcgccccggcgctgctgaaagagctgtccgaaagcgaaggcgccgttgagcgtaagctggtatatACCGGTGAAGTGAAAGCGCGTCCGGAGCGTATTACCGAATCTGAGTTCCTGTGGCAGCATAACCAGGATCCG                                                                                                                  >  SRR12117898.248900/80‑151 (MQ=33)
                                   gaccgcctgaccatcgccccggcgctgctgaaagagctgtccgaaagcgaaggcgccgttgagcgtaagctggtatatACCGGTGAAGTGAAAGCGCGTCCGGAGCGTATTACCGAATCTGAGTTCCTGTGGCAGCATAACCAGGATCCG                                                                                                                  >  SRR12117898.554965/79‑150 (MQ=33)
                                    ACCGCCTGACCATCGCCCCGGCGCTGCTGAAAGAGCTGTCCGAAAGCGAAGGCGCCGTTGAGCGTAAGCTGGTATATACCGGTGAAGTGAAAGCGCGTCCGGAGCGTATTACCGAATCTGAGTTCCTGTGGCAGCATGACCAGGATCCGAT                                                                                                                <  SRR12117898.996561/151‑1 (MQ=13)
                                        TCTGACCATCGCACCGGCACTGCTGAAAGAGCTGGCGGAGAGCGAAGGGGCTATCGAACGTAAACTGTCTTACACCGGCGAAGTGAAAGCGCGTCCGGCGCGTATCACTGAGTCCGAGTTCCTGTGGCAGCACAACCAGGATCCAATGGC                                                                                                             >  SRR12117898.661507/1‑150 (MQ=60)
                                          tgaccatcgccccggcgctgctgaaagagctgtccgaaagcgaaggcgccgttgagcgtaagctggtatatACCGGTGAAGTGAAAGCGCGTCCGGAGCGTATTACCGAATCTGAGTTCCTGTGGCAGCATAACCAGGATCCGATGGCGGT                                                                                                          >  SRR12117898.1010957/72‑151 (MQ=32)
                                           gaccatcgccccggcgctgctgaaagagctgtccgaaagcgaaggcgccgttgagcgtaagctggtatatACCGGTGAAGTGAAAGCGCGTCCGGAGCGTATTACCGAATCTGAGTTCCTGTGGCAGCATAACCAGGATCCGATGGCGGT                                                                                                          <  SRR12117898.864987/80‑1 (MQ=32)
                                            accatcgccccggcgctgctgaaagagctgtccgaaagcgaaggcgccgttgagcgtaagctggtatatACCGGTGAAGTGAAAGCGCGTCCGGAGCGTATTACCGAATCTGAGTTCCTGTGGCAGCATAACCAGGATCCGATGGCGGT                                                                                                          >  SRR12117898.74440/70‑149 (MQ=32)
                                             CCATCGCACCGGCACTGCTGAAAGAGCTGGCGGAGAGCGAAGGGGCTATCGAACGTAAACTGTCTTACACCGGCGAAGTGAAAGCGCGTCCGGCGCGTATCACTGAGTCCGAGTTCCTGTGGCAGCACAACCAGGATCCAATGGCAGTAGA                                                                                                       <  SRR12117898.844428/151‑1 (MQ=60)
                                              catcgccccggcgctgctgaaagagctgtccgaaagcgaaggcgccgttgagcgtaagctggtatatACCGGTGAAGTGAAAGCGCGTCCGGAGCGTATTACCGAATCTGAGTTCCTGTGGCAGCATAACCAGGATCCGATGGCGGTAGAC                                                                                                      >  SRR12117898.88671/68‑151 (MQ=33)
                                                 cgccccggcgctgctgaaagagctgtccgaaagcgaaggcgccgttgagcgtaagctggtatatACCGGTGAAGTGAAAGCGCGTCCGGAGCGTATTACCGAATCTGAGTTCCTGTGGCAGCATAACCAGGATCCGATGGCGGTAGACAA                                                                                                    >  SRR12117898.1192827/65‑150 (MQ=32)
                                                 cgccccggcgctgctgaaagagctgtccgaaagcgaaggcgccgttgagcgtaagctggtatatACCGGTGAAGTGAAAGCGCGTCCGGAGCGTATTACCGAATCTGAGTTCCTGTGGCAGCATAACCAGGATCCGATGGCGGTAGACAA                                                                                                    >  SRR12117898.1048591/65‑150 (MQ=32)
                                                  gccccggcgctgctgaaagagctgtccgaaagcgaaggcgccgttgagcgtaagctggtatatACCGGTGAAGTGAAAGCGCGTCCGGAGCGTATTACCGAATCTGAGTTCCTGTGGCAGCATAACCAGGATCCGATGGCGGTAGACAAAC                                                                                                  <  SRR12117898.1145290/88‑1 (MQ=32)
                                                   ccccggcgctgctgaaagagctgtccgaaagcgaaggcgccgttgagcgtaagctggtatatACCGGTGAAGTGAAAGCGCGTCCGGAGCGTATTACCGAATCTGAGTTCCTGTGGCAGCATAACCAGGATCCGATGGCGGTAGACAAACT                                                                                                 >  SRR12117898.353348/63‑151 (MQ=33)
                                                   ccccggcgctgctgaaagagctgtccgaaagcgaaggcgccgttgagcgtaagctggtatatACCGGTGAAGTGAAAGCGCGTCCGGAGCGTATTACCGAATCTGAGTTCCTGTGGCAGCATAACCAGGATCCGATGGCGGTAGACAAACT                                                                                                 >  SRR12117898.459596/63‑151 (MQ=33)
                                                   ccccggcgctgctgaaagagctgtccgaaagcgaaggcgccgttgagcgtaagctggtatatACCGGTGAAGTGAAAGCGCGTCCGGAGCGTATTACCGAATCTGAGTTCCTGTGGCAGCATAACCAGGATCCGATGGCGGTAGACAAACT                                                                                                 <  SRR12117898.705572/89‑1 (MQ=33)
                                                    cccggcgctgctgaaagagctgtccgaaagcgaaggcgccgttgagcgtaagctggtatatACCGGTGAAGTGAAAGCGCGTCCGGAGCGTATTACCGAATCTGAGTTCCTGTGGCAGCATAACCAGGATCCGATGGCGGTAGACAAACTG                                                                                                >  SRR12117898.100887/62‑151 (MQ=35)
                                                    cccggcgctgctgaaagagctgtccgaaagcgaaggcgccgttgagcgtaagctggtatatACCGGTGAAGTGAAAGCGCGTCCGGAGCGTATTACCGAATCTGAGTTCCTGTGGCAGCATAACCAGGATCCGATGGCGGTAGACAAACTG                                                                                                >  SRR12117898.411410/62‑151 (MQ=35)
                                                    cccggcgctgctgaaagagctgtccgaaagcgaaggcgccgttgagcgtaagctggtatatACCGGTGAAGTGAAAGCGCGTCCGGAGCGTATTACCGAATCTGAGTTCCTGTGGCAGCATAACCAGGATCCGATGGCGGTAGACAAACTG                                                                                                >  SRR12117898.1107632/62‑151 (MQ=35)
                                                      cggcgctgctgaaagagctgtccgaaagcgaaggcgccgttgagcgtaagctggtatatACCGGTGAAGTGAAAGCGCGTCCGGAGCGTATTACCGAATCTGAGTTCCTGTGGCAGCATAACCAGGATCCGATGGCGGTAGACAAACTGGC                                                                                              >  SRR12117898.174460/60‑151 (MQ=38)
                                                         cgctgctgaaagagctgtccgaaagcgaaggcgccgttgagcgtaagctggtatatACCGGTGAAGTGAAAGCGCGTCCGGAGCGTATTACCGAATCTGAGTTCCTGTGGCAGCATAACCAGGATCCGATGGCGGTAGACAAACTGGCGG                                                                                            >  SRR12117898.431716/57‑150 (MQ=42)
                                                          gctgctgaaagagctgtccgaaagcgaaggcgccgttgagcgtaagctggtatatACCGGTGAAGTGAAAGCGCGTCCGGAGCGTATTACCGAATCTGAGTTCCTGTGGCAGCATAACCAGGATCCGATGGCGGTAGACAAACTGGCGGA                                                                                           >  SRR12117898.395328/56‑150 (MQ=44)
                                                          ACTGCTGAAAGAGCTGGCGGAGAGCGAAGGGGCTATCGAACGTAAACTGTCTTACACCGGCGAAGTGAAAGCGCGTCCGGCGCGTATCACTGAGTCCGAGTTCCTGTGGCAGCACAACCAGGATCCAATGGCAGTAGATAAACTGGCGGA                                                                                           >  SRR12117898.1064725/1‑150 (MQ=60)
                                                           ctgctgaaagagctggccgaaagcgaaggcgccgttgagcgtaagctggtatatACCGGTGAAGTGAAAGCGCGTCCGGAGCGTATTACCGAATCTGAGTTCCTGTGGCAGCATAACCAGGATCCGATGGCGGTAGACAAACTGGCGGA                                                                                           >  SRR12117898.672060/55‑149 (MQ=44)
                                                            tgctgaaagagctgtccgaaagcgaaggcgccgttgagcgtaagctggtatatACCGGTGAAGTGAAAGCGCGTCCGGAGCGTATTACCGAATCTGAGTTCCTGTGGCAGCATAACCAGGATCCGATGGCGGTAGACAAACTGGCGGAAGG                                                                                        >  SRR12117898.924748/54‑151 (MQ=49)
                                                             gctgaaagagctgtccgaaagcgaaggcgccgttgagcgtaagctggtatatACCGGTGAAGTGAAAGCGCGTCCGGAGCGTATTACCGAATCTGAGTTCCTGTGGCAGCATAACCAGGATCCGATGGCGGTAGACAAACTGGCGGAAGGT                                                                                       <  SRR12117898.781365/99‑1 (MQ=50)
                                                                 aaagagctgtccgaaagcgaaggcgccgttgagcgtaagctggtatatACCGGTGAAGTGAAAGCGCGTCCGGAGCGTATTACCGAATCTGAGTTCCTGTGGCAGCATAACCAGGATCCGATGGCGGTAGACAAACTGGCGGAAGGTATCC                                                                                   <  SRR12117898.38708/103‑1 (MQ=57)
                                                                 aaagagctgtccgaaagcgaaggcgccgttgagcgtaagctggtatatACCGGTGAAGTGAAAGCGCGTCCGGAGCGTATTACCGAATCTGAGTTCCTGTGGCAGCATAACCAGGATCCGATGGCGGTAGACAAACTGGCGGAAGGTATCC                                                                                   >  SRR12117898.400044/49‑151 (MQ=57)
                                                                  aagagctgtccgaaagcgaaggcgccgttgagcgtaagctggtatatACCGGTGAAGTGAAAGCGCGTCCGGAGCGTATTACCGAATCTGAGTTCCTGTGGCAGCATAACCAGGATCCGATGGCGGTAGACAAACTGGCGGAAGGTATCCG                                                                                  <  SRR12117898.573468/104‑1 (MQ=59)
                                                                   agagctgtccgaaagcgaaggcgccgttgagcgtaagctggtatatACCGGTGAAGTGAAAGCGCGTCCGGAGCGTATTACCGAATCTGAGTTCCTGTGGCAGCATAACCAGGATCCGATGGCGGTAGACAAACTGGCGGAAGGTATCCGT                                                                                 <  SRR12117898.297243/105‑1 (MQ=60)
                                                                   agagctgtccgaaagcgaaggcgccgttgagcgtaagctggtatatACCGGTGAAGTGAAAGCGCGTCCGGAGCGTATTACCGAATCTGAGTTCCTGTGGCAGCATAACCAGGATCCGATGGCGGTAGACAAACTGGCGGAAGGTATCCGT                                                                                 >  SRR12117898.1077646/47‑151 (MQ=60)
                                                                   agagctgtccgaaagcgaaggcgccgttgagcgtaagctggtatatACCGGTGAAGTGAAAGCGCGTCCGGAGCGTATTACCGAATCTGAGTTCCTGTGGCAGCATAACCAGGATCCGATGGCGGTAGACAAACTGGCGGAAGGTATCCGT                                                                                 <  SRR12117898.189090/105‑1 (MQ=60)
                                                                    gagctgtccgaaagcgaaggcgccgttgagcgtaagctggtatatACCGGTGAAGTGAAAGCGCGTCCGGAGCGTATTACCGAATCTGAGTTCCTGTGGCAGCATAACCAGGATCCGATGGCGGTAGACAAACTGGCGGAAGGTATCCGT                                                                                 >  SRR12117898.853865/46‑150 (MQ=60)
                                                                    GAGCTGGCGGAGAGCGAAGGGGCTATCGAACGTAAACTGTCTTACACCGGCGAAGTGAAAGCGCGTCCGGCGCGTATCACTGAGTCCGAGTTCCTGTGGCAGCACAACCAGGATCCAATGGCAGTAGATAAACTGGCGGAAGGTATCCGTA                                                                                <  SRR12117898.791652/151‑1 (MQ=60)
                                                                     AGCTGGCGGAGAGCGAAGGGGCTATCGAACGTAAACTGTCTTACACCGGCGAAGTGAAAGCGCGTCCGGCGCGTATCACTGAGTCCGAGTTCCTGTGGCAGCACAACCAGGATCCAATGGCAGTAGATAAACTGGCGGAAGGTATCCGTA                                                                                >  SRR12117898.774809/1‑150 (MQ=60)
                                                                        tgtccgaaagcgaaggcgccgttgagcgtaagctggtatatACCGGTGAAGTGAAAGCGCGTCCGGAGCGTATTACCGAATCTGAGTTCCTGTGGCAGCATAACCAGGATCCGATGGCGGTAGACAAACTGGCGGAAGGTATCCGTAAGTT                                                                            >  SRR12117898.640943/42‑151 (MQ=60)
                                                                         gtccgaaagcgaaggcgccgttgagcgtaagctggtatatACCGGTGAAGTGAAAGCGCGTCCGGAGCGTATTACCGAATCTGAGTTCCTGTGGCAGCATAACCAGGATCCGATGGCGGTAGACAAACTGGCGGAAGGTATCCGTAAGTTT                                                                           >  SRR12117898.306361/41‑151 (MQ=60)
                                                                         gtccgaaagcgaaggcgccgttgagcgtaagctggtatatACCGGTGAAGTGAAAGCGCGTCCGGAGCGTATTACCGAATCTGAGTTCCTGTGGCAGCATAACCAGGATCCGATGGCGGTAGACAAACTGGCGGAAGGTATCCGTAAGTT                                                                            <  SRR12117898.122785/110‑1 (MQ=60)
                                                                         gtccgaaagcgaaggcgccgttgagcgtaagctggtatatACCGGTGAAGTGAAAGCGCGTCCGGAGCGTATTACCGAATCTGAGTTCCTGTGGCAGCATAACCAGGATCCGATGGCGGTAGACAAACTGGCGGAAGGTATCCGTAAGTT                                                                            <  SRR12117898.193582/110‑1 (MQ=60)
                                                                         gtccgaaagcgaaggcgccgttgagcgtaagctggtatatACCGGTGAAGTGAAAGCGCGTCCGGAGCGTATTACCGAATCTGAGTTCCTGTGGCAGCATAACCAGGATCCGATGGCGGTAGACAAACTGGCGGAAGGTATCCGTAAGTT                                                                            <  SRR12117898.293281/110‑1 (MQ=60)
                                                                          GCGGAGAGCGAAGGGGCTATCGAACGTAAACTGTCTTACACCGGCGAAGTGAAAGCGCGTCCGGCGCGTATCACTGAGTCCGAGTTCCTGTGGCAGCACAACCAGGATCCAATGGCAGTAGATAAACTGGCGGAAGGTATCCGTAAGTTTG                                                                          >  SRR12117898.121191/1‑151 (MQ=60)
                                                                           ccgaaagcgaaggcgccgttgagcgtaagctggtatatACCGGTGAAGTGAAAGCGCGTCCGGAGCGTATTACCGAATCTGAGTTCCTGTGGCAGCATAACCAGGATCCGATGGCGGTAGACAAACTGGCGGAAGGTATCCGTAAGTTTGC                                                                         <  SRR12117898.114023/113‑1 (MQ=60)
                                                                            GGAGAGCGAAGGGGCTATCGAACGTAAACTGTCTTACACCGGCGAAGTGAAAGCGCGTCCGGCGCGTATCACTGAGTCCGAGTTCCTGTGGCAGCACAACCAGGATCCAATGGCAGTAGATAAACTGGCGGAAGGTATCCGTAAGTTTGCT                                                                        <  SRR12117898.680199/151‑1 (MQ=60)
                                                                              aaagcgaaggcgccgttgagcgtaagctggtatatACCGGTGAAGTGAAAGCGCGTCCGGAGCGTATTACCGAATCTGAGTTCCTGTGGCAGCATAACCAGGATCCGATGGCGGTAGACAAACTGGCGGAAGGTATCCGTAAGTTTGCGAT                                                                      <  SRR12117898.1100904/116‑1 (MQ=60)
                                                                               aagcgaaggcgccgttgagcgtaagctggtatatACCGGTGAAGTGAAAGCGCGTCCGGAGCGTATTACCGAATCTGAGTTCCTGTGGCAGCATAACCAGGATCCGATGGCGGTAGACAAACTGGCGGAAGGTATCCGTAAGTTTGCgatc                                                                     <  SRR12117898.518124/117‑5 (MQ=60)
                                                                                agcgaaggcgccgttgagcgtaagctggtatatACCGGTGAAGTGAAAGCGCGTCCGGAGCGTATTACCGAATCTGAGTTCCTGTGGCAGCATAACCAGGATCCGATGGCGGTAGACAAACTGGCGGAAGGTATCCGTAAGTTTGCgatcg                                                                    >  SRR12117898.202734/34‑146 (MQ=60)
                                                                                 gcgaaggcgccgttgagcgtaagctggtatatACCGGTGAAGTGAAAGCGCGTCCGGAGCGTATTACCGAATCTGAGTTCCTGTGGCAGCATAACCAGGATCCGATGGCGGTAGACAAACTGGCGGAAGGTATCCGTAAGTTTGCgatcg                                                                    >  SRR12117898.174019/33‑145 (MQ=60)
                                                                                  cgaaggcgccgttgagcgtaagctggtatatACCGGTGAAGTGAAAGCGCGTCCGGAGCGTATTACCGAATCTGAGTTCCTGTGGCAGCATAACCAGGATCCGATGGCGGTAGACAAACTGGCGGAAGGTATCCGTAAGTTTGCGATCGAC                                                                  <  SRR12117898.619659/120‑1 (MQ=60)
                                                                                  CGAAGGGGCTATCGAACGTAAACTGTCTTACACCGGCGAAGTGAAAGCGCGTCCGGCGCGTATCACTGAGTCCGAGTTCCTGTGGCAGCACAACCAGGATCCAATGGCAGTAGATAAACTGGCGGAAGGTATCCGTAAGTTTGCTATTGAC                                                                  >  SRR12117898.357141/1‑151 (MQ=60)
                                                                                   gaaggcgccgttgagcgtaagctggtatatACCGGTGAAGTGAAAGCGCGTCCGGAGCGTATTACCGAATCTGAGTTCCTGTGGCAGCATAACCAGGATCCGATGGCGGTAGACAAACTGGCGGAAGGTATCCGTAAGTTTGCGATCGACC                                                                 >  SRR12117898.1128451/31‑151 (MQ=60)
                                                                                    aaggcgccgttgagcgtaagctggtatatACCGGTGAAGTGAAAGCGCGTCCGGAGCGTATTACCGAATCTGAGTTCCTGTGGCAGCATAACCAGGATCCGATGGCGGTAGACAAACTGGCGGAAGGTATCCGTAAGTTTGCGATCGACCA                                                                <  SRR12117898.825001/122‑1 (MQ=60)
                                                                                    aaggcgccgttgagcgtaagctggtatatACCGGTGAAGTGAAAGCGCGTCCGGAGCGTATTACCGAATCTGAGTTCCTGTGGCAGCATAACCAGGATCCGATGGCGGTAGACAAACTGGCGGAAGGTATCCGTAAGTTTGCGATCGACC                                                                 >  SRR12117898.1054696/30‑150 (MQ=60)
                                                                                     aggcgccgttgagcgtaagctggtatatACCGGTGAAGTGAAAGCGCGTCCGGAGCGTATTACCGAATCTGAGTTCCTGTGGCAGCATAACCAGGATCCGATGGCGGTAGACAAACTGGCGGAAGGTATCCGTAAGTTTGCGATCGACCAG                                                               <  SRR12117898.427282/123‑1 (MQ=60)
                                                                                     aggcgccgttgagcgtaagctggtatatACCGGTGAAGTGAAAGCGCGTCCGGAGCGTATTACCGAATCTGAGTTCCTGTGGCAGCATAACCAGGATCCGATGGCGGTAGACAAACTGGCGGAAGGTATCCGTAAGTTTGCGATCGACC                                                                 >  SRR12117898.773659/29‑149 (MQ=60)
                                                                                        cgccgttgagcgtaagctggtatatACCGGTGAAGTGAAAGCGCGTCCGGAGCGTATTACCGAATCTGAGTTCCTGTGGCAGCATAACCAGGATCCGATGGCGGTAGACAAACTGGCGGAAGGTATCCGTAAGTTTGCGATCGACCAGGAA                                                            <  SRR12117898.820065/126‑1 (MQ=60)
                                                                                        cgccgttgagcgtaagctggtatatACCGGTGAAGTGAAAGCGCGTCCGGAGCGTATTACCGAATCTGAGTTCCTGTGGCAGCATAACCAGGATCCGATGGCGGTAGACAAACTGGCGGAAGGTATCAGTAAGTTTGCGATCGACCAGGAA                                                            <  SRR12117898.660938/126‑1 (MQ=60)
                                                                                         GCTATCGAACGTAAACTGTCTTACACCGGCGAAGTGAAAGCGCGTCCGGCGCGTATCACTGAGTCCGAGTTCCTGTGGCAGCACAACCAGGATCCAATGGCAGTAGATAAACTGGCGGAAGGTATCCGTAAGTTTGCTATTGACCAGGAA                                                            >  SRR12117898.1046714/1‑150 (MQ=60)
                                                                                           TATCGAACGTAAACTGTCTTACACCGGCGAAGTGAAAGCGCGTCCGGCGCGTATCACTGAGTCCGAGTTCCTGTGGCAGCACAACCAGGATCCAATGGCAGTAGATAAACTGGCGGAAGGTATCCGTAAGTTTGCTATTGACCAGGAAAA                                                          >  SRR12117898.392772/1‑150 (MQ=60)
                                                                                            gttgagcgtaagctggtatatACCGGTGAAGTGAAAGCGCGTCCGGAGCGTATTACCGAATCTGAGTTCCTGTGGCAGCATAACCAGGATCCGATGGCGGTAGACAAACTGGCGGAAGGTATCCGTAAGTTTGCGATCGACCAGGAAAAAC                                                        >  SRR12117898.447195/22‑151 (MQ=60)
                                                                                            ATCGAACGTAAACTGTCTTACACCGGCGAAGTGAAAGCGCGTCCGGCGCGTATCACTGAGTCCGAGTTCCTGTGGCAGCACAACCAGGATCCAATGGCAGTAGATAAACTGGCGGAAGGTATCCGTAAGTTTGCTATTGACCAGGAAAAAC                                                        >  SRR12117898.761656/1‑151 (MQ=60)
                                                                                            ATCGAACGTAAACTGTCTTACACCGGCGAAGTGAAAGCGCGTCCGGCGCGTATCACTGAGTCCGAGTTCCTGTGGCAGCACAACCAGGATCCAATGGCAGTAGATAAACTGGCGGAAGGTATCCGTAAGTTTGCTATTGACCAGGAAAAAC                                                        <  SRR12117898.698838/151‑1 (MQ=60)
                                                                                               gagcgtaagctggtatatACCGGTGAAGTGAAAGCGCGTCCGGAGCGTATTACCGAATCTGAGTTCCTGTGGCAGCATAACCAGGATCCGATGGCGGTAGACAAACTGGCGGAAGGTATCCGTAAGTTTGCGATCGACCAGGAAAAACTGG                                                     >  SRR12117898.567027/19‑151 (MQ=60)
                                                                                               GAACGTAAACTGTCTTACACCGGCGAAGTGAAAGCGCGTCCGGCGCGTATCACTGAGTCCGAGTTCCTGTGGCAGCACAACCAGGATCCAATGGCAGTAGATAAACTGGCGGAAGGTATCCGTAAGTTTGCTATTGACCAGGAAAAACTGG                                                     >  SRR12117898.984205/1‑151 (MQ=60)
                                                                                                 ACGTAAACTGTCTTACACCGGCGAAGTGAAAGCGCGTCCGGCGCGTATCACTGAGTCCGAGTTCCTGTGGCAGCACAACCAGGATCCAATGGCAGTAGATAAACTGGCGGAAGGTATCCGTAAGTTTGCTATTGACCAGGAAAAACTGGA                                                    >  SRR12117898.626699/1‑150 (MQ=60)
                                                                                                  cgtaagctggtatatACCGGTGAAGTGAAAGCGCGTCCGGAGCGTATTACCGAATCTGAGTTCCTGTGGCAGCATAACCAGGATCCGATGGCGGTAGACAAACTGGCGGAAGGTATCCGTAAGTTTGCGATCGACCAGGAAAAACTGGAA                                                   >  SRR12117898.1061538/16‑150 (MQ=60)
                                                                                                     aagctggtatatACCGGTGAAGTGAAAGCGCGTCCGGAGCGTATTACCGAATCTGAGTTCCTGTGGCAGCATAACCAGGATCCGATGGCGGTAGACAAACTGGCGGAAGGTATCCGTAAGTTTGCGATCGACCAGGAAAAACTGGAAAAA                                                <  SRR12117898.96870/138‑1 (MQ=60)
                                                                                                     AAACTGTCTTACACCGGCGAAGTGAAAGCGCGTCCGGCGCGTATCACTGAGTCCGAGTTCCTGTGGCAGCACAACCAGGATCCAATGGCAGTAGATAAACTGGCGGAAGGTATCCGTAAGTTTGCTATTGACCAGGAAAAACTGGAAAAA                                                <  SRR12117898.1138748/150‑1 (MQ=60)
                                                                                                      agctggtatatACCGGTGAAGTGAAAGCGCGTCCGGAGCGTATTACCGAATCTGAGTTCCTGTGGCAGCATAACCAGGATCCGATGGCGGTAGACAAACTGGCGGAAGGTATCCGTAAGTTTGCGATCGACCAGGAAAAACTGGAAAAAAT                                              <  SRR12117898.890187/140‑1 (MQ=60)
                                                                                                      agctggtatatACCGGTGAAGTGAAAGCGCGTCCGGAGCGTATTACCGAATCTGAGTTCCTGTGGCAGCATAACCAGGATCCGATGGCGGTAGACAAACTGGCGGAAGGTATCCGTAAGTTTGCGATCGACCAGGAAAAACTGGAAAAAAT                                              >  SRR12117898.1077501/12‑151 (MQ=60)
                                                                                                      agctggtatatACCGGTGAAGTGAAAGCGCGTCCGGAGCGTATTACCGAATCTGAGTTCCTGTGGCAGCATAACCAGGATCCGATGGCGGTAGACAAACTGGCGGAAGGTATCCGTAAGTTTGCGATCGACCAGGAAAAACTGGAAAAAAT                                              <  SRR12117898.735891/140‑1 (MQ=60)
                                                                                                       ACTGTCTTACACCGGCGAAGTGAAAGCGCGTCCGGCGCGTATCACTGAGTCCGAGTTCCTGTGGCAGCACAACCAGGATCCAATGGCAGTAGATAAACTGGCGGAAGGTATCCGTAAGTTTGCTATTGACCAGGAAAAACTGGAAAAAATG                                             <  SRR12117898.69602/151‑1 (MQ=60)
                                                                                                         tggtatatACCGGTGAAGTGAAAGCGCGTCCGGAGCGTATTACCGAATCTGAGTTCCTGTGGCAGCATAACCAGGATCCGATGGCGGTAGACAAACTGGCGGAAGGTATCCGTAAGTTTGCGATCGACCAGGAAAAACTGGAAAAAATGAT                                           >  SRR12117898.1172315/9‑151 (MQ=60)
                                                                                                         tggtatatACCGGTGAAGTGAAAGCGCGTCCGGAGCGTATTACCGAATCTGAGTTCCTGTGGCAGCATAACCAGGATCCGATGGCGGTAGACAAACTGGCGGAAGGTATCCGTAAGTTTGCGATCGACCAGGAAAAACTGGAAAAAATGAT                                           >  SRR12117898.577794/9‑151 (MQ=60)
                                                                                                         TGTCTTACACCGGCGAAGTGAAAGCGCGTCCGGCGCGTATCACTGAGTCCGAGTTCCTGTGGCAGCACAACCAGGATCCAATGGCAGTAGATAAACTGGCGGAAGGTATCCGTAAGTTTGCTATTGACCAGGAAAAACTGGAAAAAATGAT                                           >  SRR12117898.168802/1‑151 (MQ=60)
                                                                                                          ggtatatACCGGTGAAGTGAAAGCGCGTCCGGAGCGTATTACCGAATCTGAGTTCCTGTGGCAGCATAACCAGGATCCGATGGCGGTAGACAAACTGGCGGAAGGTATCCGTAAGTTTGCGATCGACCAGGAAAAACTGGAAAAAATGA                                            <  SRR12117898.65661/142‑1 (MQ=60)
                                                                                                             atatACCGGTGAAGTGAAAGCGCGTCCGGAGCGTATTACCGAATCTGAGTTCCTGTGGCAGCATAACCAGGATCCGATGGCGGTAGACAAACTGGCGGAAGGTATCCGTAAGTTTGCGATCGACCAGGAAAAACTGGAAAAAATGATCGAC                                       <  SRR12117898.981583/147‑1 (MQ=60)
                                                                                                              TATACCGGTGAAGTGAAAGCGCGTCCGGAGCGTATTACCGAATCTGAGTTCCTGTGGCAGCATAACCAGGATCCGATGGCGGTAGACAAACTGGCGGAAGGTATCCGTAAGTTTGCGATCGACCAGGAAAAACTGGAAAAAATGATCGACG                                      >  SRR12117898.1029640/1‑151 (MQ=60)
                                                                                                              TACACCGGCGAAGTGAAAGCGCGTCCGGCGCGTATCACTGAGTCCGAGTTCCTGTGGCAGCACAACCAGGATCCAATGGCAGTAGATAAACTGGCGGAAGGTATCCGTAAGTTTGCTATTGACCAGGAAAAACTGGAAAAAATGATCGGC                                       <  SRR12117898.904499/150‑1 (MQ=60)
                                                                                                               ATACCGGTGAAGTGAAAGCGCGTCCGGAGCGTATTACCGAATCTGAGTTCCTGTGGCAGCATAACCAGGATCCGATGGCGGTAGACAAACTGGCGGAAGGTATCCGTAAGTTTGCGATCGACCAGGAAAAACTGGAAAAAATGATCGACG                                      <  SRR12117898.386487/150‑1 (MQ=60)
                                                                                                               ATACCGGTGAAGTGAAAGCGCGTCCGGAGCGTATTACCGAATCTGAGTTCCTGTGGCAGCATAACCAGGATCCGATGGCGGTAGACAAACTGGCGGAAGGTATCCGTAAGTTTGCGATCGACCAGGAAAAACTGGAAAAAATGATCGACG                                      <  SRR12117898.730567/150‑1 (MQ=60)
                                                                                                               ACACCGGCGAAGTGAAAGCGCGTCCGGCGCGTATCACTGAGTCCGAGTTCCTGTGGCAGCACAACCAGGATCCAATGGCAGTAGATAAACTGGCGGAAGGTATCCGTAAGTTTGCTATTGACCAGGAAAAACTGGAAAAAATGATCGGCG                                      <  SRR12117898.1068566/150‑1 (MQ=60)
                                                                                                                  CCGGCGAAGTGAAAGCGCGTCCGGCGCGTATCACTGAGTCCGAGTTCCTGTGGCAGCACAACCAGGATCCAATGGCAGTAGATAAACTGGCGGAAGGTATCCGTAAGTTTGCTATTGACCAGGAAAAACTGGAAAAAATGATCGGCGATCT                                  <  SRR12117898.409267/151‑1 (MQ=60)
                                                                                                                     GTGAAGTGAAAGCGCGTCCGGAGCGTATTACCGAATCTGAGTTCCTGTGGCAGCATAACCAGGATCCGATGGCGGTAGACAAACTGGCGGAAGGTATCCGTAAGTTTGCGATCGACCAGGAAAAACTGGAAAAAATGATCGACGAACTGCT                               <  SRR12117898.950614/151‑1 (MQ=60)
                                                                                                                     GTGAAGTGAAAGCGCGTCCGGAGCGTATTACCGAATCTGAGTTCCTGTGGCAGCATAACCAGGATCCGATGGCGGTAGACAAACTGGCGGAAGGTATCCGTAAGTTTGCGATCGACCAGGAAAAACTGGAAAAAATGATCGACGAACTGCT                               <  SRR12117898.670836/151‑1 (MQ=60)
                                                                                                                       GAAGTGAAAGCGCGTCCGGCGCGTATCACTGAGTCCGAGTTCCTGTGGCAGCACAACCAGGATCCAATGGCAGTAGATAAACTGGCGGAAGGTATCCGTAAGTTTGCTATTGACCAGGAAAAACTGGAAAAAATGATCGGCGATCTGCTGT                             <  SRR12117898.220649/151‑1 (MQ=60)
                                                                                                                       GAAGTGAAAGCGCGTCCGGAGCGTATTACCGAATCTGAGTTCCTGTGGCAGCATAACCAGGATCCGATGGCGGTAGACAAACTGGCGGAAGGTATCCGTAAGTTTGCgatc                                                                     >  SRR12117898.673049/1‑107 (MQ=60)
                                                                                                                        AAGTGAAAGCGCGTCCGGAGCGTATTACCGAATCTGAGTTCCTGTGGCAGCATAACCAGGATCCGATGGCGGTAGACAAACTGGCGGAAGGTATCCGTAAGTTTGCGATCGACCAGGAAAAACTGGAAAAAATGATCGACGAACTGCTGTA                            <  SRR12117898.645130/151‑1 (MQ=60)
                                                                                                                        AAGTGAAAGCGCGTCCGGAGCGTATTACCGAATCTGAGTTCCTGTGGCAGCATAACCAGGATCCGATGGCGGTAGACAAACTGGCGGAAGGTATCCGTAAGTTTGCGATCGACCAGGAAAAACTGGAAAAAATGATCGACGAACTGCTGT                             >  SRR12117898.600467/1‑150 (MQ=60)
                                                                                                                         AGTGAAAGCGCGTCCGGAGCGTATTACCGAATCTGAGTTCCTGTGGCAGCATAACCAGGATCCGATGGCGGTAGACAAACTGGCGGAAGGTATCCGTAAGTTTGCGATCGACCAGGAAAAACTGGAAAAAATGATCGACGAACTGCTGTAA                           <  SRR12117898.1062112/151‑1 (MQ=60)
                                                                                                                          GTGAAAGCGCGTCCGGAGCGTATTACCGAATCTGAGTTCCTGTGGCAGCATAACCAGGATCCGATGGCGGTAGACAAACTGGCGGAAGGTATCCGTAAGTTTGCGATCGACCAGGAAAAACTGGAAAAAATGATCGACGAAC                                   >  SRR12117898.1173774/1‑142 (MQ=60)
                                                                                                                           TGAAAGCGCGTCCGGAGCGTATTACCGAATCTGAGTTCCTGTGGCAGCATAACCAGGATCCGATGGCGGTAGACAAACTGGCGGAAGGTATCCGTAAGTTTGCGATCGACCAGGAAAAACTGGAAAAAATGATCGACGAACTGCTGTA                            >  SRR12117898.1125091/1‑148 (MQ=60)
                                                                                                                           TGAAAGCGCGTCCGGAGCGTATTACCGAATCTGAGTTCCTGTGGCAGCATAACCAGGATCCGATGGCGGTAGACAAACTGGCGGAAGGTATCCGTAAGTTTGCGATCGACCAGGAAAAACTGGAAAAAATGATCGACGAACTGCTGTA                            >  SRR12117898.740411/1‑148 (MQ=60)
                                                                                                                            GAAAGCGCGTCCGGAGCGTATTACCGAATCTGAGTTCCTGTGGCAGCATAACCAGGATCCGATGGCGGTAGACAAACTGGCGGAAGGTATCCGTAAGTTTGCGATCGACCAGGAAAAACTGGAATAAATGATCGACGAACTGCTGTAAG                          >  SRR12117898.663611/1‑149 (MQ=60)
                                                                                                                            GAAAGCGCGTCCGGAGCGTATTACCGAATCTGAGTTCCTGTGGCAGCATAACCAGGATCCGATGGCGGTAGACAAACTGGCGGAAGGTATCCGTAAGTTTGCGATCGACCAGGAAAAACTGGAAAAAATGATCGACGAACTGCTGTAAga                         <  SRR12117898.852601/150‑3 (MQ=60)
                                                                                                                             AAAGCGCGTCCGGAGCGTATTACCGAATCTGAGTTCCTGTGGCAGCATAACCAGGATCCGATGGCGGTAGACAAACTGGCGGAAGGTATCCGTAAGTTTGCGATCGACCAGGAAAAACTGGAAAAAATGATCGACGAACTGCTGTAAgaga                       <  SRR12117898.976529/151‑5 (MQ=60)
                                                                                                                                 CGCGTCCGGAGCGTATTACCGAATCTGAGTTCCTGTGGCAGCATAACCAGGATCCGATGGCGGTAGACAAACTGGCGGAAGGTATCCGTAAGTTTGCGATCGACCAGGAAAAACTGGAAAAAATGATCGACGAACTGCTGTAAgagaat                     >  SRR12117898.999851/1‑143 (MQ=60)
                                                                                                                                   CGTCCGGAGCGTATTACCGAATCTGAGTTCCTGTGGCAGCATAACCAGGATCCGATGGCGGTAGACAAACTGGCGGAAGGTATCCGTAAGTTTGCGATCGACCAGGAAAAACTGGAAAAAATGATCGACGAACTGCTGTAAgagaataga                  >  SRR12117898.124196/1‑141 (MQ=60)
                                                                                                                                    GTCCGGAGCGTATTACCGAATCTGAGTTCCTGTGGCAGCATAACCAGGATCCGATGGCGGTAGACAAACTGGCGGAAGGTATCCGTAAGTTTGCGATCGACCAGGAAAAACTGGAAAAAATGATCGACGAACTGCTGTAAgagaatagaa                 >  SRR12117898.1094622/1‑140 (MQ=60)
                                                                                                                                      CCGGAGCGTATTACCGAATCTGAGTTCCTGTGGCAGCATAACCAGGATCCGATGGCGGTAGACAAACTGGCGGAAGGTATCCGTAAGTTTGCGATCGACCAGGAAAAACTGGAAAAAATGATCGACGAGCTGCTGTAAgagaatagaaag               <  SRR12117898.422158/150‑13 (MQ=60)
                                                                                                                                                  ACTGAGTCCGAGTTCCTGTGGCAGCACAACCAGGATCCAATGGCAGTAGATAAACTGGCGGAAGGTATCCGTAAGTTTGCTATTGACCAGGAAAAACTGGAAAAAATGATCGGCGATCTGCTGTAATCATTCTTAGCGTGACCGGGAAGTC  >  SRR12117898.845060/1‑151 (MQ=60)
                                                                                                                                                    |                                                                                                                                                    
GTAACATCGGCGAAATTCTGGAACTGGCAGGCTGCGACCGTCTGACCATCGCACCGGCACTGCTGAAAGAGCTGGCGGAGAGCGAAGGGGCTATCGAACGTAAACTGTCTTACACCGGCGAAGTGAAAGCGCGTCCGGCGCGTATCACTGAGTCCGAGTTCCTGTGGCAGCACAACCAGGATCCAATGGCAGTAGATAAACTGGCGGAAGGTATCCGTAAGTTTGCTATTGACCAGGAAAAACTGGAAAAAATGATCGGCGATCTGCTGTAATCATTCTTAGCGTGACCGGGAAGTC  >  CP009273/8920‑9216

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑