Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F50 I1 R2	775	327.3	10718052	97.2%	10417946	138.8

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	4,354,806	A→G	99 (TAA→TAG)	ghoS →	type V toxin‑antitoxin system endoribonuclease antitoxin GhoS

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	4,354,806	0	A	G	100.0%	358.0 / NA	106	99 (TAA→TAG)	ghoS	type V toxin‑antitoxin system endoribonuclease antitoxin GhoS
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (30/76); total (30/76)

Only 100 of 106 total aligned reads displayed.

CTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTCAAATGCTTAGACTTACCGGATATTGATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAACCAGGAAGGATAAGAAGAACATAAAAAATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACATGAGAAAACACATATTCGTTTACTTA  >  NZ_CP025268/4354671‑4354939

catCGCAATAACGACATCGTTAGCCAAAAGTGAAATCTAAGATTTAATCACGCTCAAACTCTTAAACTCACCGCATCTTGATTTTGCCCTCCTCATTATAACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAGCCag                                                                                                                                   <  2:4645781/138‑1 (MQ=255)

 ttCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTCAAATGCTTAGACTTACCGGATATTGATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAGCCagg                                                                                                                                  <  1:2500359/140‑1 (MQ=255)

 ttCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTCAAATGCTTAGACTTACCGGATATTGATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAGCCagg                                                                                                                                  <  2:3590682/140‑1 (MQ=255)

 ttCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTCAAATGCTTAGACTTACCGGATATTGATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAGCCagg                                                                                                                                  >  2:4125011/1‑140 (MQ=255)

  tCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTCAAATGCTTAGACTTACCGGATATTGATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAGCCagga                                                                                                                                 <  1:3650967/140‑1 (MQ=255)

  tCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTCAAATGCTTAGACTTACCGGATATTGATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAGCCagga                                                                                                                                 <  1:3781339/140‑1 (MQ=255)

  tCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTCAAATGCTTAGACTTACCGGATATTGATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAGCCagga                                                                                                                                 <  1:3781674/140‑1 (MQ=255)

  tCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTCAAATGCTTAGACTTACCGGATATTGATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAGCCagga                                                                                                                                 <  2:167118/140‑1 (MQ=255)

  tCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTCAAATGCTTAGACTTACCGGATATTGATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAGCCagga                                                                                                                                 <  2:2516945/140‑1 (MQ=255)

  tCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTCAAATGCTTAGACTTACCGGATATTGATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAGCCagga                                                                                                                                 <  2:4356978/140‑1 (MQ=255)

  tCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTCAAATGCTTAGACTTACCGGATATTGATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAGCCagga                                                                                                                                 <  2:560250/140‑1 (MQ=255)

  tCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTCAAATGCTTAGACTTACCGGATATTGATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAGCCagga                                                                                                                                 <  2:673936/140‑1 (MQ=255)

  tCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTCAAATGCTTAGACTTACCGGATATTGATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAGCCagga                                                                                                                                 <  2:695640/140‑1 (MQ=255)

  tCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTCAAATGCTTAGACTTACCGGATATTGATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAGCCagga                                                                                                                                 <  1:1412819/140‑1 (MQ=255)

   cGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTCAAATGCTTAGACTTACCGGATATTGATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAGCCaggaa                                                                                                                                >  2:4812098/1‑140 (MQ=255)

          aCGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTCAAATGCTTAGACTTACCGGATATTGATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAGCCAGGAAGGATaag                                                                                                                         <  1:4114339/140‑1 (MQ=255)

            gACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTCAAATGCTTAGACTTACCGGATATTGATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAGCCAGGAAGGATaagaa                                                                                                                       <  2:971265/140‑1 (MQ=255)

                tCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTCAAATGCTTAGACTTACCGGATATTGATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAGCCAGGAAGGATaag                                                                                                                         >  1:2000011/1‑134 (MQ=255)

                tCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTCAAATGCTTAGACTTACCGGATATTGATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAGCCAGGAAGGATaag                                                                                                                         <  2:2000097/134‑1 (MQ=255)

                tCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTCAAATGCTTAGACTTACCGGATATTGATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAGCCAGGAAGGATaag                                                                                                                         >  1:2000097/1‑134 (MQ=255)

                tCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGATCAAATGCTTAGACTTACCGGATATTGATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAGCCAGGAAGGATaag                                                                                                                         <  2:2000011/134‑1 (MQ=255)

                 cGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTCAAATGCTTAGACTTACCGGATATTGATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAGCCAGGAAGGATAAGAAGAACa                                                                                                                  <  1:3009995/140‑1 (MQ=255)

                 cGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTCAAATGCTTAGACTTACCGGATATTGATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAGCCAGGAAGGATAAGAAGAACa                                                                                                                  <  1:3010587/140‑1 (MQ=255)

                   ttAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTCAAATGCTTAGACTTACCGGATATTGATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAGCCAGGAAGGATAAGAAGAACATa                                                                                                                >  1:3460384/1‑140 (MQ=255)

                      gCCGCAAGTGAAATCGAAGATTTAATCAGGCTCAAATGCTTAGACTTACCGGATATTGATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAGCCAGGAAGGATAAGAAGAACATaaaa                                                                                                             >  2:3308157/1‑140 (MQ=255)

                      gCCGCAAGTGAAATCGAAGATTTAATCAGGCTCAAATGCTTAGACTTACCGGATATTGATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAGCCAGGAAGGATAAGAAGAACATaaaa                                                                                                             <  1:641677/140‑1 (MQ=255)

                      gCCGCAAGTGAAATCGAAGATTTAATCAGGCTCAAATGCTTAGACTTACCGGATATTGATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAGCCAGGAAGGATAAGAAGAACATaaaa                                                                                                             <  1:1442337/140‑1 (MQ=255)

                        cGCAAGTGAAATCGAAGATTTAATCAGGCTCAAATGCTTAGACTTACCGGATATTGATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAGCCAGGAAGGATAAGAAGAACATaaaaaa                                                                                                           <  1:1891851/140‑1 (MQ=255)

                        cGCAAGTGAAATCGAAGATTTAATCAGGCTCAAATGCTTAGACTTACCGGATATTGATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAGCCAGGAAGGATAAGAAGAACATaaaaaa                                                                                                           <  2:3460384/140‑1 (MQ=255)

                        cGCAAGTGAAATCGAAGATTTAATCAGGCTCAAATGCTTAGACTTACCGGATATTGATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAGCCAGGAAGGATAAGAAGAACATaaaaaa                                                                                                           <  1:1703061/140‑1 (MQ=255)

                        cGCAAGTGAAATCGAAGATTTAATCAGGCTCAAATGCTTAGACTTACCGGATATTGATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAGCCAGGAAGGATAAGAAGAACATaaaaaa                                                                                                           <  2:2069320/140‑1 (MQ=255)

                        cGCAAGTGAAATCGAAGATTTAATCAGGCTCAAATGCTTAGACTTACCGGATATTGATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAGCCAGGAAGGATAAGAAGAACATaaaaaa                                                                                                           <  2:646196/140‑1 (MQ=255)

                        cGCAAGTGAAATCGAAGATTTAATCAGGCTCAAATGCTTAGACTTACCGGATATTGATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAGCCAGGAAGGATAAGAAGAACATaaaaaa                                                                                                           <  2:1187996/140‑1 (MQ=255)

                           aaGTGAAATCGAAGATTTAATCAGGCTCAAATGCTTAGACTTACCGGATTATGATTTTGACCTCAACATTATGACAGATGATGACTATTTCCGTCAGTTTTACAAGTAGCCAGGAAGGATAAGAAGAACATAAAAAATgg                                                                                                        <  2:4281841/140‑1 (MQ=255)

                             gTGAAATCGAAGATTTAATCAGGCTCAAATGCTTAGACTTACCGGATATTGATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAGCCAGGAAGGATAAGAAGAACATAAAAAATGGCa                                                                                                      <  1:789015/140‑1 (MQ=255)

                               gAAATCGAAGATTTAATCAGGCTCAAATGCTTAGACTTACCGGATATTGATTTTGACCTCAACGTTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAGCCAGGAAGGATAAGAAGAACATAAAAAATGGCACt                                                                                                    <  1:4896371/140‑1 (MQ=255)

                                       aGATTTAATCAGGCTCAAATGCTTAGACTTACCGGATATTGATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAGCCAGGAAGGATAAGAAGAACATAAAAAATGGCACTATTCTCTa                                                                                            <  2:5315373/140‑1 (MQ=255)

                                             aaTCAGGCTCAAATGCTTAGACTTACCGGATATTGATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAGCCAGGAAGGATAAGAAGAACATAAAAAATGGCACTATTCTCTAAAatat                                                                                      >  2:1972137/1‑140 (MQ=255)

                                                       aaaTGCTTAGACTTACCGGATATTGATTTTGACCTCAACATTATGACAGTTGCTGAATATTTCCGTCAGTTTTACAAGTAGCCAGGAAGGATAAGAAGAACATAAAAAATGGCACTATTCTCTAAAATATTAATTTTTTa                                                                            <  1:3815900/140‑1 (MQ=255)

                                                       aaaTGCTTAGACTTACCGGATATTGATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAGCCAGGAAGGATAAGAAGAACATAAAAAATGGCACTATTCTCTAAAATATTAATTTTTTa                                                                            <  1:4384644/140‑1 (MQ=255)

                                                            cTTAGACTTACCGGATATTGATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAGCCAGGAAGGATAAGAAGAACATAAAAAATGGCACTATTCTCTAAAATATTAATTTTTTATGTGa                                                                       <  2:85383/140‑1 (MQ=255)

                                                            cTTAGACTTACCGGATATTGATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAGCCAGGAAGGATAAGAAGAACATAAAAAATGGCACTATTCTCTAAAATATTAATTTTTTATGTGa                                                                       <  1:962756/140‑1 (MQ=255)

                                                              tAGACTTAGCCGATATTGATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAGCCAGGAAGGATAAGAAGTACATAAAAAATGGCACTATTGTCTAAAATATTAATTTTTTATGTGAtt                                                                     <  2:3923420/140‑1 (MQ=255)

                                                              tAGACTTACCAGATATTGATTTTGACCTCAACATTATGACAGTTGATGACTATTTCTGTCAGTTTTACAAGTAGCCAGGAAGGATAAGAAGCACATAAAAAATGGCACTATTTTCTAAAATATTAATTTTTTATGTGAtt                                                                     <  2:3923579/140‑1 (MQ=255)

                                                                 aCTTACCGGATATTGACTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGATTTACAAGTAGCCAGGAAGGATAAGAAGAACATAAAAAATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGgt                                                                  <  1:2945575/140‑1 (MQ=255)

                                                                 aCTTACCGGATATTGAATTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAGCCAGGAAGGATAAGAAGAACATAAAAAGTGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGgt                                                                  <  1:2957589/140‑1 (MQ=255)

                                                                  cTTACCGGATATTGATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAGCCAGGAAGGATAAGAAGAACATAAAAAATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGgtg                                                                 <  1:1920234/140‑1 (MQ=255)

                                                                  cTTACCGGATATTGATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAGCCAGGAAGGATAAGAAGAACATAAAAAATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGgtg                                                                 <  1:4072494/140‑1 (MQ=255)

                                                                  cTTACCGGATATTGATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAGCCAGGAAGGATAAGAAGAACATAAAAAATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGgtg                                                                 <  2:1925684/140‑1 (MQ=255)

                                                                  cTTACCGGATATTGATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAGCCAGGAAGGATAAGAAGAACATAAAAAATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGgtg                                                                 <  2:4065996/140‑1 (MQ=255)

                                                                   ttACCGGATATTGCTTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAGCCAGGAAGGATAAGAAGAACATAAAAAATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGgtgt                                                                <  1:4706191/140‑1 (MQ=255)

                                                                   ttACCGGATATTGATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAGCCAGGAAGGATAAGAAGAACATAAAAAATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGgtgt                                                                >  1:1846951/1‑140 (MQ=255)

                                                                     cccGGATATTGATTTTGACCTAAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTAGAAGTAGCCAGGAAGGATAAGAAGAACATAAAAAATGGCACTATTCTCTAACATATTAATTTTATATGTGATCGGTGTGa                                                              >  2:4439859/2‑140 (MQ=255)

                                                                     aCCGGCTATTGATTTTGACCTCAACATTATGACAGTTGATGACTATTTCAGTCAGTTTTACAAGTAGCCAGGAAGGATAAGAAGAACATAAAACATGGCACTATTCTCTAAAATATTAATTCTTTATGCGATAGATGTGa                                                              >  2:2710954/1‑140 (MQ=255)

                                                                     aCCGGATATTGATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAGCCAGGAAGGATAAGAAGAACATAAAAAATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGa                                                              >  2:2934883/1‑140 (MQ=255)

                                                                     aCCGGATATTGATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAGCCAGGAAGGATAAGAAGAACATAAAAAATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGa                                                              >  1:4354906/1‑140 (MQ=255)

                                                                     aCCGGATATTGATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAGCCAGGAAGGATAAGAAGAACATAAAAAATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGa                                                              >  1:5091226/1‑140 (MQ=255)

                                                                     aCCGGATATTGATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAGCCAGGAAGGATAAGAAGAACATAAAAAATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGa                                                              >  1:5090901/1‑140 (MQ=255)

                                                                      ccGGATATTGATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAGCCAGGAAGGATAAGAAGAACATAAAAAATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGgtg                                                                 >  2:3734457/1‑136 (MQ=255)

                                                                      ccGGATATTGATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAGCCAGGAAGGATAAGAAGAACATAAAAAATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGgtg                                                                 <  1:3734457/136‑1 (MQ=255)

                                                                         gATATTGATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAGCCAGGAAGGATAAGAAGAACATAAAAAATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACat                                                          <  1:562121/140‑1 (MQ=255)

                                                                          atatTGATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAGCCAGGAAGGATAAGAAGAACATAAAAAATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACata                                                         <  2:1597497/140‑1 (MQ=255)

                                                                              tGATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAGCCAGGAAGGATAAGAAGAACATAAAAAATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCt                                                     <  2:504784/140‑1 (MQ=255)

                                                                               gATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAGCCAGGAAGGATAAGAAGAACATAAAAAATGGCACTATTCTCTAAAATATTAATTTTTTATGTGAt                                                                      <  1:3611180/122‑1 (MQ=255)

                                                                               gATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAGCCAGGAAGGATAAGAAGAACATAAAAAATGGCACTATTCTCTAAAATATTAATTTTTTATGTGAt                                                                      >  2:3611180/1‑122 (MQ=255)

                                                                                 ttttGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAGCCAGGAAGGATAAGAAGAACATAAAAAATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTg                                                  <  1:1859593/140‑1 (MQ=255)

                                                                                   ttGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAGCCAGGAAGGATAAGAAGAACATAAAAAATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTc                                                <  1:1387812/140‑1 (MQ=255)

                                                                                       ccTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAGCCAGGAAGGATAAGAAGAACATAAAAAATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTa                                            <  1:3216/140‑1 (MQ=255)

                                                                                        cTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAGCCAGGAAGGATAAGAAGAACATAAAAAATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTAt                                           <  2:2695263/140‑1 (MQ=255)

                                                                                        cTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAGCCAGGAAGGATAAGAAGAACATAAAAAATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTAt                                           <  1:2675450/140‑1 (MQ=255)

                                                                                          cAATATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAGCCAGGAAGGATACGAAGAACATAAAAAATGGCACTATTCTCTAAAATATTAATTTTTTATGTTATTGGTGTGAACATATCCTCTGTCATTATCt                                         >  2:2271872/1‑140 (MQ=255)

                                                                                           caCATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAGCCAGGAAGGATAAGAAGAACATAAAAAATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTg                                        >  1:1957346/2‑140 (MQ=255)

                                                                                                   gACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAGCCAGGAAGGATAAGAAGAACATAAAAAATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTAtct                                <  1:2263508/140‑1 (MQ=255)

                                                                                                   gACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAGCCAGGAAGGATAAGAAGAACATAAAAAATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTAtct                                <  1:2934883/140‑1 (MQ=255)

                                                                                                     cAGTTGATGACTATTTCCGTCAGTTTTACAAGTAGCCAGGAAGGATACGAAGAACATAAAAAATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTca                              >  2:4201081/1‑140 (MQ=255)

                                                                                                       gTTGATGACTATTTCCGTCAGTTTTACAAGTAGCCAGGAAGGATAAGAAGAACATAAAAAATGGCACTATTGTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTcaca                            >  2:4930270/1‑140 (MQ=255)

                                                                                                       gTTGATGACTATTTCCGTCAGTTTTACAAGTAGCCAGGAAGGATAAGAAGAACATAAAAAATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTcaca                            <  2:1628311/140‑1 (MQ=255)

                                                                                                       gTTGATGACTATTTCCGTCAGTTGTAAAAGTAGCCAGGAAGGATAAGAAGAACATAAAAAATGGCACTATTCTCTAAAATATTAATTTTTAATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTcaca                            >  1:3191513/1‑140 (MQ=255)

                                                                                                        ttGATGACTATTTCCGTCAGTTTTACAAGTAGCCAGGAAGGATAAGAAGAACATAAAAAATGGCa                                                                                                      >  2:1709010/1‑65 (MQ=255)

                                                                                                        ttGATGACTATTTCCGTCAGTTTTACAAGTAGCCAGGAAGGATAAGAAGAACATAAAAAATGGCa                                                                                                      <  1:1709010/65‑1 (MQ=255)

                                                                                                        ttGATGACTATTTCCGTCAGTTTTACAAGTAGCCAGGAAGGATAAGAAGAACATAAAAAATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACAt                           <  2:3693277/140‑1 (MQ=255)

                                                                                                          gatgaCTATTTCCGTCAGTTTTACAAGTAGCCAGGAAGGATAAGAAGAACATAAAAAATGGCACTATTCTCTAAAATATTAATTTTCTATGTGATTGGTGTGAATATATCCTTTGTCATTATCTGGTTTATCTCACATga                         >  1:3237706/1‑140 (MQ=255)

                                                                                                           atgaCTATTTCCGTCAGTTTTACAAGTAGCCAGGAAGGATAAGAAGAACATAAAAAATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACATgag                        <  1:3928119/140‑1 (MQ=255)

                                                                                                             gaCTATTTCCGTCAGTTTTACAAGTAGCCAGGAAGGATAAGAAGAACATAAAAAATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACATGAGaa                      <  1:145429/140‑1 (MQ=255)

                                                                                                              tgtATTTCCGTCAGTTTTACAAGTAGCCAGGAAGGATAAGAAGAACATATAAAATGGCACTATGCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACATGAGaaa                     <  2:1171684/138‑1 (MQ=255)

                                                                                                                tATTTCCGTCAGTTTTACAAGTAGCCAGGAAGGATAAGAAGAACATAAAAAATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTCTGAACATATCCTTTGTCATTATCTGGTTTATCTCACATGAGAAAac                   <  2:4648132/140‑1 (MQ=255)

                                                                                                                tATTGCCGTCAGTCTTACAAGTAGCCAGGAAGGATAAGAAGAACATAAAAAATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTCTGAACATAGCCTTTGTCATTATCTGGTTTATCTCACATGAGAAAac                   <  2:4648902/140‑1 (MQ=255)

                                                                                                                     ccGTCAGTTTTACAAGTAGCCAGGAAGGATAAGAAGAACATAAAAAATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACATGAGAAAACACata              <  1:776635/140‑1 (MQ=255)

                                                                                                                        tCAGTTTTACAAGTAGCCGGGAAGGATAAGAAGAACATAAAAAATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACATGAGAAAACACATATTc           >  2:4671120/1‑140 (MQ=255)

                                                                                                                        tCAGTTTTACAAGTAGCCAGGAAGGATAAGAAGAACATAAAAAATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACATGAGAAAACACATATTc           >  1:4364469/1‑140 (MQ=255)

                                                                                                                        tCAGTTTTACAAGTAGCCAGGAAGGATAAGAAGAACATAAAAAATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACATGAGAAAACACATATTc           >  1:4936142/1‑140 (MQ=255)

                                                                                                                          aGTTTTACAAGTAGCCAGGAAGGATAAGAAGAACATAAAAAATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACATGAGAAAACACATATTCGt         <  1:4812098/140‑1 (MQ=255)

                                                                                                                          aGTTTTACAAGTAGCCAGGAAGGATAAGAAGAACATAAAAAATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACATGAGAAAACACATATTCGt         >  1:148239/1‑140 (MQ=255)

                                                                                                                               tACAAGTAGCCAGGAAGGATAAGAAGAACATAAAAAATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACATGAGAAAACACATATTCGTTTACt    <  2:5090901/140‑1 (MQ=255)

                                                                                                                               tACAAGTAGCCAGGAAGGATAAGAAGAACATAAAAAATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACATGAGAAAACACATATTCGTTTACt    <  2:5091226/140‑1 (MQ=255)

                                                                                                                               tACAAGTAGCCAGGAAGGATAAGAAGAACATAAAAAATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACATGAGAAAACACATATTCGTTTACt    <  1:3067130/140‑1 (MQ=255)

                                                                                                                               tACAAGTAGCCAGGAAGGATAAGAAGAACATAAAAAATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACATGAGAAAACACATATTCGTTTACt    <  2:3407783/140‑1 (MQ=255)

                                                                                                                               tACAAGTAGCCAGGAAGGATAAGAAGAACATAAAAAATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACATGAGAAAACACATATTCGTTTACt    <  2:3407512/140‑1 (MQ=255)

                                                                                                                               tACAAGTAGCCAGGAAGGATAAGAAGAACATAAAAAATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACATGAGAAAACACATATTCGTTTACt    <  2:3407477/140‑1 (MQ=255)

                                                                                                                                 cAAGTAGCCAGGAAGGATAAGAAGAACATAAAAAATTGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACATGAGAAAACACATATTCGTTTACTTa  <  1:3532472/140‑1 (MQ=255)

CTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTCAAATGCTTAGACTTACCGGATATTGATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAACCAGGAAGGATAAGAAGAACATAAAAAATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACATGAGAAAACACATATTCGTTTACTTA  >  NZ_CP025268/4354671‑4354939

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

AATGAAAACTCCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTCAAATGCTTAGACTTACCGGATATTGATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAACCAGGAAGGATAAGAAGAACATAAAAAATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACATGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGA  >  NZ_CP025268/4354660‑4354956

AATGAAAACTCATTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTCAAATGCTTAGACTTACCGGATATTGATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAGCCAG                                                                                                                                                    <  SRR14920189.4536277/151‑1 (MQ=60)

 tttggctgtctcacacaaacaaactacccctatttttttcaagcagaagacggcatacgagattagctgcagtctcgtgggctcggagatgtgtataagagacagTTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAGCCAGG                                                                                                                                                   <  SRR14920189.835524/46‑1 (MQ=60)

   GAAAACTCCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTCAAATGCTTAGACTTACCGGATATTGATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAGCCAGGAA                                                                                                                                                 <  SRR14920189.1658078/151‑1 (MQ=60)

   GAAAACTCCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTCAAATGCTTAGACTTACCGGATATTGATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAGCCAGGAA                                                                                                                                                 <  SRR14920189.2518714/151‑1 (MQ=60)

        CTCCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTCAAATGCTTAGACTTACCGGATATTGATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAGCCAGGGAGGATA                                                                                                                                            <  SRR14920189.2293749/151‑1 (MQ=60)

        CTCCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTCAAATGCTTAGACTTACCGGATATTGATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAGCCAGGAAGGATA                                                                                                                                            <  SRR14920189.2809614/151‑1 (MQ=60)

            TTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTCAAATGCTTAGACTTACCGGATATTGATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAGCCAGGAAGGATAAGAA                                                                                                                                        <  SRR14920189.2506001/151‑1 (MQ=60)

             TCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGACGATTTAATCAGGCTCAAATGCTTAGACTTACCGGATATTGATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAGCCAGGAAGGATAAGAAG                                                                                                                                       <  SRR14920189.3790122/151‑1 (MQ=60)

             TCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTCAAATGCTTAGACTTACCGGATATTGATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAGCCAGGAAGGATAAGAAG                                                                                                                                       <  SRR14920189.1416018/151‑1 (MQ=60)

             TCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTCAAATGCTTAGACTTACCGGATATTGATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAGCCAGGAAGGATAAGAAG                                                                                                                                       <  SRR14920189.3659526/151‑1 (MQ=60)

             TCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTCAAATGCTTAGACTTACCGGATATTGATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAGCCAGGAAGGATAAGAAG                                                                                                                                       <  SRR14920189.3790457/151‑1 (MQ=60)

                CAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTCAAATGCTTAGACTTACCGGATATTGATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAATTAGCCAGGAAGGATAAGAAGAAA                                                                                                                                    >  SRR14920189.2004708/1‑151 (MQ=60)

                CAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTCAAATGCTTAGACTTACCGGATATTGATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAGCCAGGAATGATAAGAAGAAC                                                                                                                                    >  SRR14920189.2004622/1‑151 (MQ=60)

                   TAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTCAAATGCTTAGACTTACCGGATATTGATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAGCCAGGAAGGATAAGAAGAACATA                                                                                                                                 >  SRR14920189.3468610/1‑151 (MQ=60)

                     ACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTCAAATGCTTAGACTTACCGGATATTGATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAGCCAGGAAGGATAAGAAGAACATAAA                                                                                                                               <  SRR14920189.4124498/151‑1 (MQ=60)

                            CGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTCAAATGCTTAGACTTACCGGATATTGATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAGCCAGGAAGGATAAGAAGAACATAAAAAATGGC                                                                                                                        <  SRR14920189.3017122/151‑1 (MQ=60)

                            CGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTCAAATGCTTAGACTTACCGGATATTGATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAGCCAGGAAGGATAAGAAGAACATAAAAAATGGC                                                                                                                        <  SRR14920189.3017715/151‑1 (MQ=60)

                                 GCCGCAAGTGAAATCGAAGATTTAATCAGGCTCAAATGCTTAGACTTACCGGATATTGATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAGCCAGGAAGGATAAGAAGAACATAAAAAATGGCACTAT                                                                                                                   <  SRR14920189.1445625/151‑1 (MQ=60)

                                 GCCGCAAGTGAAATCGAAGATTTAATCAGGCTCAAATGCTTAGACTTACCGGATATTGATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAGCCAGGAAGGATAAGAAGAACATAAAAAATGGCACTAT                                                                                                                   <  SRR14920189.643271/151‑1 (MQ=60)

                                   CGCAAGTGAAATCGAAGATTTAATCAGGCTCAAATGCTTAGACTTACCGGATATTGATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAGCCAGGAAGGATAAGAAGAACATAAAAAATGGCACTATTC                                                                                                                 <  SRR14920189.1896189/151‑1 (MQ=60)

                                   CGCAAGTGAAATCGAAGATTTAATCAGGCTCAAATGCTTAGACTTACCGGATATTGATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAGCCAGGAAGGATAAGAAGAACATAAAAAATGGCACTATTC                                                                                                                 <  SRR14920189.1706953/151‑1 (MQ=60)

                                        agcagaagacggcatacgagattagctgcagtctcgtgggctcggagatgtgtataagagacagCATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAGCCAGGAAGGATAAGAAGAACATAAAAAATGGCACTATTCTCTAA                                                                                                            <  SRR14920189.1712918/87‑1 (MQ=60)

                                        GTGAAATCGAAGATTTAATCAGGCTCAAATGCTTAGACTTACCGGATATTGATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAGCCAGGAAGGATAAGAAGAACATAAAAAATGGCACTATTCTCTAA                                                                                                            <  SRR14920189.790952/151‑1 (MQ=60)

                                          GAAATCGAAGATTTAATCAGGCTCAAATGCTTAGACTTACCGGATATTGATTTTGACCTCAACGTTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAGCCAGGAAGGATAAGAAGAACATAAAAAATGGCACTATTCTCTAAAA                                                                                                          <  SRR14920189.4908733/151‑1 (MQ=60)

                                                   GATTTAATCAGGCTCAAATGCTTAGACTTACCGGATATTGATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAGCCAGGAAGGATAAGAAGAACATAAAAAATGGCACTATTCTCTAAAATATTAATTT                                                                                                 >  SRR14920189.85661/1‑151 (MQ=60)

                                                   GATTTAATCAGGCTCAAATGCTTAGACTTACCGGATATTGATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAGCCAGGAAGGATAAGAAGAACATAAAAAATGGCACTATTCTCTAAAATATTAATTT                                                                                                 >  SRR14920189.3887359/1‑151 (MQ=60)

                                                                  AAATGCTTAGACTTACCGGATATTGATTTTGACCTCAACATTATGACAGTTGCTGAATATTTCCGTCAGTTTTACAAGTAGCCAGGAAGGATAAGAAGAACATAAAAAATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTG                                                                                  <  SRR14920189.3824973/151‑1 (MQ=60)

                                                                  AAATGCTTAGACTTACCGGATATTGATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAGCCAGGAAGGATAAGAAGAACATAAAAAATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTG                                                                                  <  SRR14920189.4395439/151‑1 (MQ=60)

                                                                   AATGCTTAGACTTACCGGATATTGATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAGCCAGGAAGGATAAGAAGAACATAAAAAATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGT                                                                                 >  SRR14920189.1851184/1‑151 (MQ=60)

                                                                     TGCTTAGACTTACCGGATATTGATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAGCCAGGAAGGATAAGAAGAACATAAAAAATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGA                                                                               >  SRR14920189.5103896/1‑151 (MQ=60)

                                                                     TGCTTAGACTTACCGGATATTGATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAGCCAGGAAGGATAAGAAGAACATAAAAAATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGA                                                                               >  SRR14920189.4365651/1‑151 (MQ=60)

                                                                     TGCTTAGACTTACCGGATATTGATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAGCCAGGAAGGATAAGAAGAACATAAAAAATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGA                                                                               >  SRR14920189.5103571/1‑151 (MQ=60)

                                                                       CTTAGACTTACCGGATATTGATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAGCCAGGAAGGATAAGAAGAACATAAAAAATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAAC                                                                             <  SRR14920189.965019/151‑1 (MQ=60)

                                                                        gagacagTACCGGATATTGATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAGCCAGGAAGGATAAGAAGAACATAAAAAATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACA                                                                            <  SRR14920189.3619657/144‑1 (MQ=60)

                                                                            ACTTACCGGATATTGACTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGATTTACAAGTAGCCAGGAAGGATAAGAAGAACATAAAAAATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATC                                                                        <  SRR14920189.2952189/151‑1 (MQ=60)

                                                                            ACTTACCGGATATTGAATTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAGCCAGGAAGGATAAGAAGAACATAAAAAGTGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATC                                                                        <  SRR14920189.2964443/151‑1 (MQ=60)

                                                                             CTTACCGGATATTGATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAGCCAGGAAGGATAAGAAGAACATAAAAAATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCC                                                                       <  SRR14920189.3743165/151‑1 (MQ=60)

                                                                             CTTACCGGATATTGATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAGCCAGGAAGGATAAGAAGAACATAAAAAATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCC                                                                       <  SRR14920189.1924648/151‑1 (MQ=60)

                                                                             CTTACCGGATATTGATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAGCCAGGAAGGATAAGAAGAACATAAAAAATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCC                                                                       <  SRR14920189.4082521/151‑1 (MQ=60)

                                                                              TTACCGGATATTGCTTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAGCCAGGAAGGATAAGAAGAACATAAAAAATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCT                                                                      <  SRR14920189.4718019/151‑1 (MQ=60)

                                                                                    GATATTGATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAGCCAGGAAGGATAAGAAGAACATAAAAAATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCA                                                                <  SRR14920189.563563/151‑1 (MQ=60)

                                                                                           ATTTTGACCTCCACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAGCCAGGAAGGATAAGAAGAACATAAAAAATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTG                                                         >  SRR14920189.1961844/1‑151 (MQ=60)

                                                                                            TTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAGCCAGGAAGGATAAGAAGAACATAAAAAATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGG                                                        <  SRR14920189.1863853/151‑1 (MQ=60)

                                                                                              TTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAGCCAGGAAGGATAAGAAGAACATAAAAAATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTT                                                      <  SRR14920189.1390945/151‑1 (MQ=60)

                                                                                                  CCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAGCCAGGAAGGATAAGAAGAACATAAAAAATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATC                                                  <  SRR14920189.3222/151‑1 (MQ=60)

                                                                                                   CTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAGCCAGGAAGGATAAGAAGAACATAAAAAATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCT                                                 <  SRR14920189.2681443/151‑1 (MQ=60)

                                                                                                       ACATTATGACAGTTGATGACTATTTCCGTCAGTTGTAAAAGTAGCCAGGAAGGATAAGAAGAACATAAAAAATGGCACTATTCTCTAAAATATTAATTTTTAATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA                                             >  SRR14920189.3199137/1‑151 (MQ=60)

                                                                                                          TTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAGCCAGGAAGGATAAGAAGAACATAAAAAATGGCACTATTCTCTAAAATATTAATTTTCTATGTGATTGGTGTGAATATATCCTTTGTCATTATCTGGTTTATCTCACATGA                                          >  SRR14920189.3245521/1‑151 (MQ=60)

                                                                                                              GACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAGCCAGGAAGGATAAGAAGAACATAAAAAATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACATGAGAAA                                      <  SRR14920189.2941454/151‑1 (MQ=60)

                                                                                                              GACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAGCCAGGAAGGATAAGAAGAACATAAAAAATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACATGAGAAA                                      <  SRR14920189.2268706/151‑1 (MQ=60)

                                                                                                                  GTTGATGACTATTTCCGTCAGTTTTACAAGTAGCCAGGAAGGATAAGAAGAACATAAAAAATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACATGAGAAAACAC                                  <  SRR14920189.2848758/151‑1 (MQ=60)

                                                                                                                      ATGACTATTTCCGTCAGTTTTACAAGTAGCCAGGAAGGATAAGAAGAACATAAAAAATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACATGAGAAAACACATAT                              <  SRR14920189.3937849/151‑1 (MQ=60)

                                                                                                                        GACTATTTCCGTCAGTTTTACAAGTAGCCAGGAAGGATAAGAAGAACATAAAAAATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACATGAGAAAACACATATTC                            >  SRR14920189.4948578/1‑151 (MQ=60)

                                                                                                                        GACTATTTCCGTCAGTTTTACAAGTAGCCAGGAAGGATAAGAAGAACATAAAAAATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACATGAGAAAACACATATTC                            >  SRR14920189.4375231/1‑151 (MQ=60)

                                                                                                                        GACTATTTCCGTCAGTTTTACAAGTAGCCAGGAAGGATAAGAAGAACATAAAAAATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACATGAGAAAACACATATTC                            <  SRR14920189.145866/151‑1 (MQ=60)

                                                                                                                          CTATTTCCGTCAGTTTTACAAGTAGCCAGGAAGGATAAGAAGAACATAAAAAATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACATGAGAAAACACATATTCGT                          >  SRR14920189.148679/1‑151 (MQ=60)

                                                                                                                                CCGTCAGTTTTACAAGTAGCCAGGAAGGATAAGAAGAACATAAAAAATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACATGAGAAAACACATATTCGTTTACTT                    <  SRR14920189.778537/151‑1 (MQ=60)

                                                                                                                                     AGTTTTACAAGTAGCCAGGAAGGATAAGAAGAACATAAAAAATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACATGAGAAAACACATATTCGTTTACTTAGTGC               <  SRR14920189.4824296/151‑1 (MQ=60)

                                                                                                                                          TACAAGTAGCCAGGAAGGATAAGAAGAACATAAAAAATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACATGAGAAAACACATATTCGTTTACTTAGTGCATTCC          <  SRR14920189.3074401/151‑1 (MQ=60)

                                                                                                                                            CAAGTAGCCAGGAAGGATAAGAAGAACATAAAAAATTGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACATGAGAAAACACATATTCGTTTACTTAGTGCATTCCTG        <  SRR14920189.3540807/151‑1 (MQ=60)

                                                                                                                                               GTAGCCAGGAAGGATAAGAAGAACATAAAAAATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACATGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTC     >  SRR14920189.1579571/1‑151 (MQ=60)

                                                                                                                                                  GCCAGGAAGGATAAGAAGAACATAAAAAATGGCACTATTCTCTAAAATATTAATTTTTTATGctgtctcttatacacatctccgagcccacgagactgcagctaatctcgtatgccgtcttctgcttgaaaaaataacccctgcgactgtt  >  SRR14920189.3609731/1‑62 (MQ=60)

AATGAAAACTCCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTCAAATGCTTAGACTTACCGGATATTGATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAACCAGGAAGGATAAGAAGAACATAAAAAATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACATGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGA  >  NZ_CP025268/4354660‑4354956

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`