Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F50 I1 R1	782	316.7	10427156	97.0%	10114341	138.2

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	4,542,898	(A)_6→7	pseudogene (27/602 nt)	fimB →	type 1 fimbria switch DNA invertase FimB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	4,542,892	1	.	A	98.4%	470.7 / ‑0.7	125	pseudogene (21/602 nt)	fimB	type 1 fimbria switch DNA invertase FimB
Reads supporting (aligned to +/- strand): ref base . (1/1); new base A (79/44); total (80/45)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 138 total aligned reads displayed.

CCACTTACGTTAGTTATTAAGCAAACGTTCGCTTTTATAAACATAATCAGGATAAAAATGTTGGATTATTGCTAACCCAGCACAGCTAGTGCGCGTCTGTAATTATAAGGGAAAAACGATGAAGAATAAGGCTGATAA‑C‑AAAAAAGGAACTTCCTGACCCATAGTGAAATCGAATCACTCCTTAAAGCAGCAAATACCGGGCCTCATGCAGCACGTAATTATTGTCTGACTTTGCTTTGTTTTATTCATGGTTTCCGGGCGAGTGAAATTTGT  >  NZ_CP025268/4542754‑4543026

      aCGTTAGTTATTAAGCAAACGTTCGCTTTTATAAACATAATCAGGATAAAAATGTTGGATTATTGCTAACCCAGCACAGCTAGTGCGCGTCTGTAATTATAAGGGAAAAACGATGAAGAATAAGGCTGATAA‑C‑aaaaaaa                                                                                                                                 >  2:1078032/1‑139 (MQ=255)

        gttagttaTTAAGCAAACGTTCGCTTTTATAAACATAATCAGGATAAAAATGTTGGATTATTGCTAACCCAGCACAGCTAGTGCGCGTCTGTAATTATAATGGAAAAACGATGAAGAATAAGGCTGATAA‑C‑aaaaacagg                                                                                                                               >  1:4209928/1‑136 (MQ=255)

        gttagttaTTAAGCAAACGTTCGCTTTTATAAACATAATCAGGATAAAAATGTTGGATTATTGCTAACCCAGCACAGCTAGTGCGCGTCTGTAATTATAAGGGAAAAACGATGAAGAATAAGTCTGCTAA‑C‑aaaaaaagg                                                                                                                               >  1:184837/1‑137 (MQ=255)

        gttagttaTTAAGCAAACGTTCGCTTTTATAAACATAATCAGGATAAAAATGTTGGATTATTGCTAACCCAGCACAGCTAGTGCGCGTCTGTAATTATAAGGGAAAAACGATGAAGAATAAGGCTGATAA‑C‑aaaaaaagg                                                                                                                               <  2:3463849/140‑4 (MQ=255)

        gttagttaTTAAGCAAACGTTCGCTTTTATAAACATAATCAGGATAAAAATGTTGGATTATTGCTAACCCAGCACAGCTAGTGCGCGTCTGTAATTATAAGGGAAAAACGATGAAGAATAAGGCTGATAA‑C‑aaaaaaagg                                                                                                                               >  1:2082726/1‑137 (MQ=255)

         ttagttaTTAAGCAAACGTTCGCTTTTATAAACATAATCAGGATAAAAATGTTGGATTATTGCTAACCCAGCACAGCTAGTGCGCGTCTGTAATTATAAGGGAAAAACGATGAAGAATAAGGCTGATAA‑C‑aaaaaaagga                                                                                                                              <  1:3417665/140‑5 (MQ=255)

         ttagttaTTAAGCAAACGGGCGCGGTTATAAACATAATCAGGATAAAAATGTTGGGTTATAGCTAACCCAGCACAGCGAGTGCGCGTCTGTAATTATAAGGGAAAAACGATGTAGAATAAGGCGGATAA‑C‑aaaatgaggt                                                                                                                              >  2:33320/1‑134 (MQ=255)

          tagttaTTAAGCAAACGTTCGCTTTTATAAACATAATCAGGATAAAAATGTTGGATTATTGCTAACCCAGCACAGCTAGTGCGCGTCTGTAATTATAAGGGAAAAACGATGAAGAATAAGGCTGATAA‑C‑aaaaaaaggaa                                                                                                                             <  1:1433721/140‑6 (MQ=255)

             ttattaAGCAAACGTTCGCTTTTATAAACATAATCAGGATAAAAATGTTGGATTATTGCTAACCCAGCACAGCTAGTGCGCGTCTGTAATTATAAGGGAAAAACGATGAAGAATAAGGCTGATAA‑CAAAAAAAGGAACtt                                                                                                                           >  2:5161228/1‑140 (MQ=255)

                ttaAGCAAACGTTCGCTTTTATAAACATAATCAGGATAAAAATGTTGGATTATTGCTAACCCAGCACAGCTAGTGCGCGTCTGTAATTATAAGGGAAAAACGATGAAGAATAAGGCTGATAA‑CAAAAAAAGGAACTTCCt                                                                                                                        <  2:2972608/140‑1 (MQ=255)

                ttaAGCAAACGTTCGCTTTTATAAACATAATCAGGATAAAAATGTTGGATTATTACTAACCCAGCACAGCTAGTGCGCGTCTGTAATTATAAGGGAAAAACGATGAAGAATAAGGCTGATAA‑CAAAAAAAGGAACTTCCt                                                                                                                        <  2:2971017/140‑1 (MQ=255)

                  aaGCAAACGTTCGCTTTTATAAACATAATCAGGATAAAAATGTTGGATTATTGCTAACCCAGCACAGCTAGTGCGCGTCTGTAATTATAAGGGAAAAACGATGAAGAATAAGGCTGATAA‑CAAAAAAAGGAACTTCCTGa                                                                                                                      >  1:724781/1‑140 (MQ=255)

                  aaGCAAACGTTCGCTTTTATAAACATAATCAGGATAAAAATGTTGGATTATTGCTAACCCAGCACAGCTAGTGCGCGTCTGTAATTATAAGGGAAAAACGATGAAGAATAAGGCTGATAA‑CAAAAAAAGGAACTTCCTGa                                                                                                                      >  1:724800/1‑140 (MQ=255)

                    gCAAACGTTCGCTTTTATAAACATAATCAGGATAAAAATGTTGGATTATTGCTAACCCAGCACAGCTAGTGCGCGTCTGTAATTATAAGGGAAAAACGATGAAGAATAAGGCTGATAA‑CAAAAAAAGGAACTTCCTGAcc                                                                                                                    <  2:1529770/140‑1 (MQ=255)

                    gCAAACGTTCGCTTTTATAAACATAATCAGGATAAAAATGTTGGATTATTGCTAACCCAGCACAGCTAGTGCGCGTCTGTAATTATAAGGGAAAAACGATGAAGAATAAGGCTGATAA‑CAAAAAAAGGAACTTCCTGAcc                                                                                                                    <  2:1621041/140‑1 (MQ=255)

                      aaaCGTTCGCTTTTATAAACATAATCAGGATAAAAATGTTGGATTATTGCTAACCCAGCACAGCTAGTGCGCGTCTGTAATTATAAGGGAAAAACGATGAAGAATAAGGCTGATAA‑CAAAAAAAGGAACTTCCTGACCCa                                                                                                                  >  1:2263725/1‑140 (MQ=255)

                             cGCTTTTATAAACATAATCAGGATAAAAATGTTGGATTATTGCTAACCCAGCACAGCTAGTGCGCGTCTGTAATTATAAGGGAAAAACGATGAAGAATAAGGCTGATAA‑CAAAAAAAGGAACTTCCTGACCCATAGTGaa                                                                                                           >  2:1608576/1‑140 (MQ=255)

                              gCTTTTATAAACATAATCAGGATAAAAATGTTGGATTATTGCTAACCCAGCACAGCTAGTGCGCGTCTGTAATTATAAGGGAAAAACGATGAAGAATAAGGCTGATAA‑CAAAAAAAGGAACTTCCTGACCCATAGTGaaa                                                                                                          <  1:2237729/140‑1 (MQ=255)

                              gCTTTTATAAACATAATCAGGATAAAAATGATGGATTAGTGCTAACCCAGCACAGCTAGTGCGCGTCTGTAATTATAAGGGAAAAACGATGAAGAATAAGGCTGATAA‑CAAAAAAAGGAACTTCCTGACCCATAGTGaaa                                                                                                          <  2:2021752/140‑1 (MQ=255)

                                  ttATAAACATAATCAGGATAAAAATGTTGGATTATTGCTAACCCAGCACAGCTAGTGCGCGTCTGTAATTATAAGGGAAAAACGATGAAGAATAAGGCTGATAA‑CAAAAAAAGGAACTTCCTGACCCATAGTGAAATCGa                                                                                                      >  2:1882171/1‑140 (MQ=255)

                                    ataAACATAATCAGGATAAAAATGTTGGATTATTGCTAACCCAGCACAGCTAGTGCGCGTCTGTAATTATAAGGGAAAAACGATGAAGAATAAGGCTGATAA‑CAAAAAAAGGAACTTCCTGACCCATAGTGAAATCGAAt                                                                                                    >  1:3839223/1‑140 (MQ=255)

                                      aaaCATAATCAGGATAAAAATGTTGGATTATTGCTAACCCAGCACAGCTAGTGCGCGTCTGTAATTATAAGGGAAAAACGATGAAGAATAAGGCTGATAA‑CAAAAAAAGGAACTTCCTGACCCATAGTGAAATCGAATCa                                                                                                  <  1:3144985/140‑1 (MQ=255)

                                      aaaCATAATCAGGATAAAAATGTTGGATTATTGCTAACCCAGCACAGCTAGTGCGCGTCTGTAATTATAAGGGAAAAACGATGAAGAATAAGGCTGATAA‑CAAAAAAAGGAACTTCCTGACCCATAGTGAAATCGAATCa                                                                                                  <  1:1428460/140‑1 (MQ=255)

                                      aaaCATAATCAGGATAAAAATGTTGGATTATTGCTAACCCAGCACAGCTAGTGCGCGTCTGTAATTATAAGGGAAAAACGATGAAGAATAAGGCTGATAA‑CAAAAAAAGGAACTTCCTGACCCATAGTGAAATCGAATCa                                                                                                  <  2:3449588/140‑1 (MQ=255)

                                      aaaCATAATCAGGATAAAAATGTTGGATTATTGCTAACCCAGCACAGCTAGTGCGCGTCTGTAATTATAAGGGAAAAACGATGAAGAATAAGGCTGATAA‑CAAAAAAAGGAACTTCCTGACCCATAGTGAAATCGAATCa                                                                                                  <  1:5090356/140‑1 (MQ=255)

                                      aaaCATAATCAGGATAAAAATGTTGGATTATTGCTAACCCAGCACAGCTAGTGCGCGTCTGTAATTATAAGGGAAAAACGATGAACAATAAGGCTGATAA‑CAAAAAAAGGAACTTCCTGACCCATAGTGAAATCGAATCa                                                                                                  <  1:4076798/140‑1 (MQ=255)

                                            aaTCAGGATAAAAATGTTGGATTATTGCTAACCCAGCACAGCTAGTGCGCGTCTGTAATTATAAGGGAAAAACGATGAAGAATAAGGCTGATAA‑CAAAAAAAGGAACTTCCTGACCCATAGTGAAATCGAATCACTCCtt                                                                                            >  1:2892238/1‑140 (MQ=255)

                                            aaTCAGGATAAAAATGTTGGATTATTGCTAACCCAGCACAGCTAGTGCGCGTCTGTAATTATAAGGGAAAAACGATGAAGAATAAGGCTGATAA‑CAAAAAAAGGAACTTCCTGACCCATAGTGAAATCGAATCACTCCtt                                                                                            >  2:606022/1‑140 (MQ=255)

                                            aaTCAGGATAAAAAGGTTGGGCTATTTCTAACACAGCAAAGCTAGTGACCGTCTGCAAGTATAAGGGAAATACGATGAAGAATAAGGCTGAGAA‑CAAAAAAAGGAAGTTCTTGACCCATACTGAAATCGAATGACTCAtt                                                                                            >  2:447638/1‑140 (MQ=255)

                                               cAGGATAAAAATGTTGGATTATTGCTAACCCAGCACAGCTAGTGCGCGTCTGTAATTATAAGGGAAAAACGATGAAGAATAAGGCTGATAA‑CAAAAAAAGGAACTTCCTGACCCATAGTGAAATCGAATCACTCCTTaaa                                                                                         <  2:3027468/140‑1 (MQ=255)

                                                  gATAAAAATGTTGGATTATTGCTAACCCAGCACAGCTAGTGCGCGTCTGTAATTATAAGGGAAAAACGATGAAGAATAAGGCTGATAA‑CAAAAAAAGGAACTTCCTGACCCATAGTGAAATCGAATCACTCCTTAAagca                                                                                      >  2:1344664/1‑140 (MQ=255)

                                                  gATAAAAAAGTTGGAATATTGCTAACCCAAAACAGCTAGAGATCGTCTGTAGTCATAAGGGGAAAGCGATGAAGAATAAGGCTGATAA‑CAAAAAAAGGAACTACATGACCCATAGGGAAGGAGAAGCACTCCTTAAagaa                                                                                      >  2:1442834/1‑138 (MQ=255)

                                                    tAAAAATGTTGGATTATTGCTAACCCAGCACAGCTAGTGCGCGTCTGTAATTATAAGGGAAAAACGATGAAGAATAAGGCTGATAA‑CAAAAAAAGGAACTTCCTGACCCATAGTGAAATCGAATCACTCCTTAAagcagc                                                                                    <  1:2656028/140‑1 (MQ=255)

                                                    tAAAAATGTTGGATTATTGCTAACCCACCACAGCTAGTGCGCGTCTGTAATTATAAGGGAAAAACGATGAAGAATAAGGCTGATAA‑CAAAAAAAGGAACTTCCTGACCCATAGTGAAATCGAATCACTCCTTAAagcagc                                                                                    <  1:2656599/140‑1 (MQ=255)

                                                     aaaaaTGTTGGATTATTGCTAACCCAGCACAGCTAGTGCGCGTCTGTAATTATAAGGGAAAAACGATGAAGAATAAGGCTGATAA‑CAAAAAAAGGAACTTCCTGACCCATAGTGAAATCGAATCACTCCTTAAAgcagca                                                                                   <  1:4089589/140‑1 (MQ=255)

                                                       aaaTGTTGGATTATTGCTAACCCAGCACAGCTAGTGCGCGTCTGTAATTATAAGGGAAAAACGATGAAGAATAAGGCTGATAA‑CAAAAAAAGGAACTTCCTGACCCATAGTGAAATCGAATCACTCCTTAAAGCAGCaaa                                                                                 >  2:2283324/1‑140 (MQ=255)

                                                           gTTGGATTATTGCTAACCCAGCACAGCTAGTGCGCGTCTGTAATTATAAGGGAAAAACGATGAAGAATAAGGCTGATAA‑CAAAAAAAGGAACTTCCTGACCCATAGTGAAATCGAATCACTCCTTAAAGCAGCAAATAcc                                                                             <  1:1538103/140‑1 (MQ=255)

                                                           gTTGGATTATTGCTAACCCAGCACAGCTAGTGCGCGTCTGTAATTATAAGGGAAAAACGATGAAGAATAAGGCTGATAA‑CAAAAAAAGGAACTTACTGACCCATAGTGAAATCGAATCACTCCTTAAAGCAGCAAATAcc                                                                             <  1:4433206/140‑1 (MQ=255)

                                                            ttGGATTATTGCTAACCCAGCACAGCTAGTGCGCGTCTGTAATTATAAGGGAAAAACGATGAAGAATAAGGCTGATAA‑CAAAAAAAGGAACTTCCTGACCCATAGTGAAATCGAATCACTCCTTAAAGCAGCAAATACCg                                                                            >  2:2674229/1‑140 (MQ=255)

                                                            ttGGATTATTGCTAACCCAGCACAGCTAGTGCGCGTCTGTAATTATAAGGGAAAAACGATGAAGAATAAGGCTGATAA‑CAAAAAAAGGAACTTCCTGACCCATAGTGAAATCGAATCACTCCTTAAAGCAGCAAATACCg                                                                            >  1:2682092/1‑140 (MQ=255)

                                                            ttGGATTATTGCTAACCCAGCACAGCTAGTGCGCGTCTGTAATTATAAGGGAAAAACGATGAAGAATAAGGCTGATAA‑CAAAAAAAGGAACTTCCTGACCCATAGTGAAATCGAATCACTCCTTAAAGCAGCAAATACCg                                                                            >  1:3984784/1‑140 (MQ=255)

                                                            ttGGATTATTGCTAACCCAGCACAGCTAGTGCGCGTCTGTAATTATAAGGGAAAAACGATGAAGAATAAGGCTGATAA‑CAAAAAAAGGAACTTCCTGACCCATAGTGAAATCGAATCACTCCTTAAAGCAGCAAATACCg                                                                            >  1:3008701/1‑140 (MQ=255)

                                                            ttGGATTATTGCTAACCCAGCACAGCTAGTGCGCGTCTGTAATTATAAGGGAAAAACGATGAAGAATAAGGCTGATAA‑CAAAAAAAGGAACTTCCTGACCCATAGTGAAATCGAATCACTCCTTAAAGCAGCAAATACCg                                                                            >  1:3301834/1‑140 (MQ=255)

                                                               gATTATTGCTAACCCAGCACAGCTAGTGCGCGTCTGTAATTATAAGGGAAAAACGATGAAGAATAAGGCTGATAA‑CAAAAAAAGGAACTTCCTGACCCATAGTGAAATCGAATCACTCCTTAAAGCAGCAAATACCGGGc                                                                         >  2:4884281/1‑140 (MQ=255)

                                                                      gCTAACCCAGCACAGCTAGTGCGCGTCTGTAATTATAAGGGAAAAACGATGAAGAATAAGGCTGATAA‑CAAAAAAAGGAACTTCCTGACCCATAGTGAAATCGAATCACTCCTTAAAGCAGCAAATACCGGGCCTCATgc                                                                  >  1:2808698/1‑140 (MQ=255)

                                                                       ccaaCCCAGCACAGCTAGTGCGCGTCTGTAATTATAAGGGAAAAACGATGAAGAATAAGGCTGATAA‑CAAAAAAAGGAACTTCCTGACCCATAGTGAAATCGAATCACTCCTTAAAGCAGCAAATACCGGGCCTCATgca                                                                 <  1:3441298/138‑1 (MQ=255)

                                                                       cTAACCCAGCACAGCTAGTGCGCGTCTGTAATTATAAGGGAAAAACGATGAAGAATAAGGCTGATAA‑CAAAAAAAGGAACTTCCTGACCCATAGTGAAATCGAATCACTCCTTAAAGCAGCAAATACCGGGCCTCATg                                                                   <  2:3392138/138‑1 (MQ=255)

                                                                       cTAACCCAGCACAGCTAGTGCGCGTCTGTAATTATAAGGGAAAAACGATGAAGAATAAGGCTGATAA‑CAAAAAAAGGAACTTCCTGACCCATAGTGAAATCGAATCACTCCTTAAAGCAGCAAATACCGGGCCTCATg                                                                   <  2:762993/138‑1 (MQ=255)

                                                                       cTAACCCAGCACAGCTAGTGCGCGTCTGTAATTATAAGGGAAAAACGATGAAGAATAAGGCTGATAA‑CAAAAAAAGGAACTTCCTGACCCATAGTGAAATCGAATCACTCCTTAAAGCAGCAAATACCGGGCCTCATg                                                                   >  1:3392138/1‑138 (MQ=255)

                                                                         aaCCCAGCACAGCTAGTGCGCGTCTGTAATTATAAGGGAAAAACGATGAAGAATAAGGCTGATAA‑CAAAAAAAGGAACTTCCTGACCCATAGTGAAATCGAATCACTCCTTAAAGCAGCAAATACCGGGCCTCATgcagc                                                               >  1:871352/1‑140 (MQ=255)

                                                                          aCCCAGCACAGCTAGTGCGCGTCTGTAATTATAAGGGAAAAACGATGAAGAATAAGGCTGATAA‑CAAAAAAAGGAACTTCCTGACCCATAGTGAAATCGAATCACTCCTTAAAGCAGCAAATACCGGGCCTCATgcagca                                                              >  2:389441/1‑140 (MQ=255)

                                                                          aCCCAGCACAGCTAGTGCGCGTCTGTAATTATAAGGGAAAAACGATGAAGAATAAGGCTGATAA‑CAAAAAAAGGAACTTCCTGACCCATAGTGAAATCGAATCACTCCTTAAAGCAGCAAATACCGGGCCTCATgcagca                                                              <  2:2439999/140‑1 (MQ=255)

                                                                          aCCCAGCACAGCTAGTGCGCGTCTGTAATTATAAGGGAAAAACGATGAAGAATAAGGCTGATAA‑CAAAAAAAGGAACTTCCTGACCCATAGTGAAATCGAATCACTCCTTAAAGCAGCAAATACCGGGCCTCATgcagca                                                              >  1:273623/1‑140 (MQ=255)

                                                                          aCCCAGCACAGCTAGTGCGCGTCTGTAATTATAAGGGAAAAACGATGAAGAATAAGGCTGATAA‑CAAAAAAAGGAACTTCCTGACCCATAGTGAAATCGAATCACTCCTTAAAGCAGCAAATACCGGGCCTCATgcagca                                                              >  2:4775174/1‑140 (MQ=255)

                                                                          aCCCAGCACAGCTAGTGCGCGTCTGTAATGATAAGGGACAAACGATGAAGAATAAGGGTGATAA‑CAAAAAAAGGAAGTTCCTGACGCATAGTGAAATCGAAACACTCCTTAAAGCAGCAAATACCAGGCCTCATgcggaa                                                              >  2:3033469/1‑136 (MQ=255)

                                                                            ccAGCACAGCTAGTGCGCGTCTGTAATTATAAGGGAAAAACGATGAAGAATAAGGCTGATAA‑CAAAAAAAGGAACTTCCTGACCCATAGTGAAATCGAATCACTCCTTAAAGCAGCAAATACCGGGCCTCATGCAGCACg                                                            <  2:1577353/140‑1 (MQ=255)

                                                                               gCACAGCTAGTGCGCGTCTGTAATTATAAGGGAAAAACGATGAAGAATAAGGCTGATAA‑CAAAAAAAGGAACTTCCTGACCCATAGTGAAATCGAATCACTCCTTAAAGCAGCAAATACCGGGCCTCATGCAGCACGTaa                                                         >  2:4036431/1‑140 (MQ=255)

                                                                               gCACAGCTAGTGCGCGTCTGTAATTATAAGGGAAAAACGATGAAGAATAAGGCTGATAA‑CAAAAAAAGGAACTTCCTGACCCATAGTGAAATCGAATCACTCCTTAAAGCAGCAAATACCGGGCCTCATGCAGCACGTaa                                                         >  1:1449319/1‑140 (MQ=255)

                                                                                cacaGCTAGTGCGCGTCTGTAATTATAAGGGAAAAACGATGAAGAATAAGGCTGATAA‑CAAAAAAAGGAACTTCCTGACCCATAGTGAAATCGAATCACTCCTTAAAGCAGCAAATACCGGGCCTCATGCAGCACGTAat                                                        <  2:4547461/140‑1 (MQ=255)

                                                                                cacaGCTAGTGCGCGTCTGTAACTATAAGGGAAAAACGATGAAGAATAAGGCTGATAA‑CAAAAAAAGGAACTTCCTGACCCATAGTGAAATCGAATCACTCCTTAAAGCAGCAAATACCGGGCCTCATGCAGCACGTAat                                                        >  2:3535811/1‑140 (MQ=255)

                                                                                 acaGCTAGTGCGCGTCTGTAATTATAAGGGAAAAACGATGAAGAATAAGGCTGATAA‑CAAAAAAAGGAACTTCCTGACCCATAGTGAAATCGAATCACTCCTTAAAGCAGCAAATACCGGGCCTCATGCAGCACGTAatt                                                       >  2:1878477/1‑140 (MQ=255)

                                                                                  caGCTAGTGCGCGTCTGTAATTATAAGGGAAAAACGATGAAGAATAAGGCTGATAA‑CAAAAAAAGGAACTTCCTGACCCATAGTGAAATCGAATCACTCCTTAAAGCAGCAAATACCGGGCCTCATGCAGCACGTAatta                                                      <  2:3487428/140‑1 (MQ=255)

                                                                                  caGCTAGTGCGCGTCTGTAATTATAAGGGAAAAACGATGAAGAATAAGGCTGATAA‑CAAAAAAAGGAACTTCCTGACCCATAGTGAAATCGAATCACTCCTTAAAGCAGCAAATACCGGGCCTCATGCAGCACGTAatta                                                      <  1:1883864/140‑1 (MQ=255)

                                                                                  caGCTAGTGCGCGTCTGTAATTATAAGGGAAAAACGATGAAGAATAAGGCTGATAA‑CAAAAAAAGGAACTTCCTGACCCATAGTGAAATCGAATCACTCCTTAAAGCAGCAAATACCGGGCCTCATGCAGCACGTAatta                                                      <  1:1884168/140‑1 (MQ=255)

                                                                                     cTAGTGCGCGTCTGTAATTATAAGGGAAAAACGATGAAGAATAAGGCTGATAA‑CAAAAAAAGGAACTTCCTGACCCATAGTGAAATCGAATCACTCCTTAAAGCAGCAAATACCGGGCCTCATGCAGCACGTAattattt                                                   >  1:211253/1‑139 (MQ=255)

                                                                                     cTAGTGCGCGTCTGTAATTATAAGGGAAAAACGATGAAGAATAAGGCTGATAA‑CAAAAAAAGGAACTTCCTGACCCATAGTGAAATCGAATCACTCCTTAAAGCAGCAAATACCGGGCCTCATGCAGCACGTAATTATTg                                                   >  1:1746445/1‑140 (MQ=255)

                                                                                     cTAGTGCGCGTCTGTAATTATAAGGGAAAAACGATGAAGAATAAGGCTGATAA‑CAAAAAAAGGAACTTCCTGACCCATAGTGAAATCGAATCACTCCTTAAAGCAGCAAATACCGGGCCTCATGCAGCACGTAATTATTg                                                   >  1:1732205/1‑140 (MQ=255)

                                                                                     cTAGTGCGCGTCTGTAATTATAAGGGAAAAACGATGAAGAATAAGGCTGATAA‑CAAAAAAAGGAACTTCCTGACCCATAGTGAAATCGAATCACTCCTTAAAGCAGCAAATACCGGGCCTCATGCAGCACGTAATTATTg                                                   >  2:2969634/1‑140 (MQ=255)

                                                                                     cTAGTGCGCGTCTGTAATTATAAGGGAAAAACGATGAAGAATAAGGCTGATAA‑CAAAAAAAGGAACTTCCTGACCCATAGTGAAATCGAATCACTCCTTAAAGCAGCAAATACCGGGCCTCATGCAGCACGTAATTATTg                                                   >  1:4243836/1‑140 (MQ=255)

                                                                                     cTAGTGCGCGTCTGTAATTATAAGGGAAAAACGATGAAGAATAAGGCTGATAA‑CAAAAAAAGGAACTTCCTGACCCATAGTGAAATCGAATCACTCCTTAAAGCAGCAAATACCGGGCCTCATGCAGCACGTAATTATTg                                                   >  1:211287/1‑140 (MQ=255)

                                                                                     cTAGTGCGCGTCTGTAATTATAAGGGAAAAACGATGAAGAATAAGGCTGATAA‑CAAAAAAAGGAACTTCCTGACCCATAGTGAAATCGAATCACTCCTTAAAGCAGCAAATACCGGGCCTCATGCAGCACGTAATTATTg                                                   >  1:3519620/1‑140 (MQ=255)

                                                                                     cTAGTGCGCGTCTGTAATTATAAGGGAAAAACGATGAAGAATAAGGCTGATAA‑CAAAAAAAGGAACTTCCTGACCCATAGTGAAATCGAATCACTCCTTAAAGCAGCAAATACCGGGCCTCATGCAGCACGTAATTATTg                                                   >  1:2794663/1‑140 (MQ=255)

                                                                                         tGCGCGTCTGTAATTATAAGGGAAAAACGATGAAGAATAAGGCTGATAA‑CAAAAAAAGGAACTTCCTGACCCATAGTGAAATCGAATCACTCCTTAAAGCAGCAAATACCGGGCCTCATGCAGCACGTAATTATTGTCTg                                               >  1:833043/1‑140 (MQ=255)

                                                                                             cgTCTGTAATTATAAGGGAAAAACGATGAAGAATAAGGCTGATAA‑CAAAAAAAGGAACTTCCTGACCCATAGTGAAATCGAATCACTCCTTAAAGCAGCAAATACCGGGCCTCATGCAGCACGTAATTATTGTCTGActt                                           >  2:1231046/1‑140 (MQ=255)

                                                                                             cgTCTGTAATTATAAGGGAAAAACGATGAAGAATAAGGCTGATAA‑CAAAAAAAGGAACTTCCTGACCCATAGTGAAATCGAATCACTCCTTAAAGCAGCAAATACCGGGCCTCATGCAGCACGTAATTATTGTCTGActt                                           >  2:1124172/1‑140 (MQ=255)

                                                                                               tCTGTAATTATAAGGGAAAAACGATGAAGAATAAGGCTGATAA‑CAAAAAAAGGAACTTCCTGACCCATAGTGAAATCGAATCACTCCTTAAAGCAGCAAATACCGGGCCTCATGCAGCACGTAATTATTGTCTGActttg                                         >  1:1592230/1‑140 (MQ=255)

                                                                                               tCTGTAATTATAAGGGAAAAACGATGAAGAATAAGGCTGATAA‑CAAAAAAAGGAACTTCCTGACCCATAGTGAAATCGAATCACTCCTTAAAGCAGCAAATACCGGGCCTCATGCAGCACGTAATTATTGTCTGActttg                                         >  1:1624609/1‑140 (MQ=255)

                                                                                               tCTGTAATTATAAGGGAAAAACGATGAAGAATAAGGCTGATAA‑CAAAAAAAGGAACTTCCTGACCCATAGTGAAATCGAATCACTCCTTAAAGCAGCAAATACCGGGCCTCATGCAGCACGTAATTATTGTCTGActttg                                         >  1:4662505/1‑140 (MQ=255)

                                                                                                 tGTAATTATAAGGGAAAAACGATGAAGAATAAGGCTGATAA‑CAAAAAAAGGAACTTCCTGACCCATAGTGAAATCGAATCACTCCTTAAAGCAGCAAATACCGGGCCTCATGCAGCACGTAATTATTGTCTGActttgct                                       <  2:880162/140‑1 (MQ=255)

                                                                                                 tGTAATTATAAGGGAAAAACGATGAAGAATAAGGCTGATAA‑CAAAAAAAGGAACTTCCTGACCCATAGTGAAATCGAATCACTCCTTAAAGCAGCAAATACCGGGCCTCATGCAGCACGTAATTATTGTCTGActttgct                                       <  2:1392398/140‑1 (MQ=255)

                                                                                                 tGTAATTATAAGGGAAAAACGATGAAGAATAAGGCTGATAA‑CAAAAAAAGGAACTTCCTGACCCATAGTGAAATCGAATCACTCCTTAAAGCAGCAAATACCGGGCCTCATGCAGCACGTAATTATTGTCTGActttgct                                       <  1:3509585/140‑1 (MQ=255)

                                                                                                  gTAATTATAAGGGAAAAACGATGAAGAATAAGGCTGATAA‑CAAAAAAAGGGACTTCCTGACCCATAGTGAAATCGAATCACTCCTTAAAGCAGCAAATACCGGGCCTCATGCAGCACGTAATTATTGTCTGActttgctt                                      >  2:4029248/1‑140 (MQ=255)

                                                                                                  gTAATTATAAGGGAAAAACGATGAAGAATAAGGCTGATAA‑CAAAAAAAGGAACTTCCTGACCCATAGTGAAATCGAATCACTCCTTAAAGCAGCAAATACCGGGCCTCATGCAGCACGTAATTATTGTCTGActttgctt                                      <  2:2694660/140‑1 (MQ=255)

                                                                                                        ataAGGGAAAAACGATGAAGAATAAGGCTGATAA‑CAAAAAAAGGAACTTCCTGACCCATAGTGAAATCGAATCACTCCTTAAAGCAGCAAATACCGGGCCTCATGCAGCACGTAATTATTGTCTGACTTTGCTTTGtttt                                >  1:3897719/1‑140 (MQ=255)

                                                                                                         taAGGGAAAAACGATGAAGAATAAGGCTGATAACC‑AAAAAAGGAACTTCCTGACCCATCGTGAAATCGAATCACTCCTTAAAGCAGCAAATACCGGGCCTCATGCAGCACGTAATTATTGTCTGACTTTGCTTTGTTTTa                               <  1:4307583/140‑1 (MQ=255)

                                                                                                          aaGGGAAAAACGATGAAGAATAAGGGTGATAAGA‑AAAAAAGGAACTTCCTGACCCCTGGTGAAATCGAATCACTCCTTAAAGCAGCAAATACCGGGCCTAATGCAGCAAGTAACTCTTGTCTGACTTTGGTTTGTGTTAt                              >  2:3275391/1‑140 (MQ=255)

                                                                                                          aaGGGAAAAACGATGAAGAATAAGGCTGATAA‑CAAAAAAAGGAACTTCCTGACCCATAGTGAAATCGAATCACTCCTTAAAGCAGCAAATACCGGGCCTCATGCAGCACGTAATTATTGTCTGACTTTGCTTTGTTTTAt                              >  2:1823026/1‑140 (MQ=255)

                                                                                                          aaGGGAAAAACGATGAAGAATAAGGCTGATAA‑CAAAAAAAGGAACTTCCTGACCCATAGTGAAATCGAATCACTCCTTAAAGCAGCAAATACCGGGCCTCATGCAGCACGTAATTATTGTCTGACTTTGCTTTGTTTTAt                              >  1:637797/1‑140 (MQ=255)

                                                                                                          aaGGGAAAAACGATGAAGAATAAGGCTGATAA‑CAAAAAAAGGAACTTCCTGACCCATAGTGAAATCGAATCACTCCTTAAAGCAGCAAATACCGGGCCTCATGCAGCACGTAATTATTGTCTGACTTTGCTTTGTTTTAt                              >  2:147927/1‑140 (MQ=255)

                                                                                                             ggAAAAACGATGAAGAATAAGGCTGATAA‑CAAAAAAAGGAACTTCCTGACCCATAGTGAAATCGAATCACTCCTTAAAGCAGCAAATACCGGGCCTCATGCAGCACGTAATTATTGTCTGACTTTGCTTTGTTTTATTCa                           <  1:4884281/140‑1 (MQ=255)

                                                                                                             ggAAAAACGATGAAGAATAAGGCTGATAA‑CAAAAAAAGGAACTTCCTGACCCATAGTGAAATCGAATCACTCCTTAAAGCAGCAAATACCGGGCCTCATGCAGCACGTAATTATTGTCTGACTTTGCTTTGTTTTATTCa                           <  2:641827/140‑1 (MQ=255)

                                                                                                               aaaaaCGATGAAGAATAAGGCTGATAA‑CAAAAAAAGGAACTTCCTGACCCATAGTGAAATCGAATCACTCCTTAAAGCAGCAAATACCGGGCCTCATGCAGCACGTAATTATTGTCTGACTTTGCTTTGTTTTATTCATg                         <  1:4775174/140‑1 (MQ=255)

                                                                                                                 aaaCGATGAAGAATGAGGCTGATAA‑CAAAAAAAGGAACTTCCTGACCCATAGTGAAATCGAATCACTCCTTAAAGCAGCAAATACCGGGCCTCATGCAGCACGTAATTATTGTCTGACTTTGCTTTGTTTTATTCATGGt                       >  1:457404/1‑140 (MQ=255)

                                                                                                                       tGAAGAATAAGGCTGATAA‑CAAAAAAAGGAACTGCCTGACCCATAGTGAAATCGAATCACTCCTTAAAGCAGCAAATACCGGGCCTCATGCAGCACGTAATTATTGTCTGACTTGGCTTTGTTTTATTCATGGTTTCCgg                 >  2:1765967/1‑140 (MQ=255)

                                                                                                                         aagaaTAAGGCTGATAA‑CAAAAAAAGGAACTTCCTGACCCATAGTGAAATCGAATCACTCCTTAAAGCAGCAAATACCGGGCCTCATGCAGCACGTAATTATTGTCTGACTTTGCTTTGTTTTATTCATGGTTTCCGGGc               >  1:862867/1‑140 (MQ=255)

                                                                                                                              tAAGGCTGATAA‑CAAAAAAAGGAACTTCCTGACCCATAGTGAAATCGAATCACTCCTTAAAGCAGCAAATACCGGGCCTCATGCAGCACGTAATTATTGTCTGACTTTGCTTTGTTTTATTCATGGTTTCCGGGCGAGTg          >  2:4270034/1‑140 (MQ=255)

                                                                                                                                      aTAA‑CAAAAAAAGGAACTTCCTGACCCATAGTGAAATCGAATCACTCCTTAAAGCAGCAAATACCGGGCCTCATGCAGCACGTAATTATTGTCTGACTTTGCTTTGTTTTATTCATGGTTTCCGGGCGAGTGAAATTTGt  >  2:4006593/1‑140 (MQ=255)

                                                                                                                                      aTAA‑CAAAAAAAGGAACTTCCTGACCCATAGTGAAATCGAATCACTCCTTAAAGCAGCAAATACCGGGCCTCATGCAGCACGTAATTATTGTCTGACTTTGCTTTGTTTTATTCATGGTTTCCGGGCGAGTGAAATTTGt  >  1:4847417/1‑140 (MQ=255)

                                                                                                                                      aTAA‑CAAAAAAAGGAACTTCCTGACCCATAGTGAAATCGAATCACTCCTTAAAGCAGCAAATACCGGGCCTCATGCAGCACGTAATTATTGTCTGACTTTGCTTTGTTTTATTCATGGTTTCCGGGCGAGTGAAATTTGt  >  1:3127515/1‑140 (MQ=255)

                                                                                                                                      aTAA‑CAAAAAAAGGAACTTCCTGACCCATAGTGAAATCGAATCACTCCTTAAAGCAGCAAATACCGGGCCTCATGCAGCACGTAATTATTGTCTGACTTTGCTTTGTTTTATTCATGGTTTCCGGGCGAGTGAAATTTGt  >  2:1147506/1‑140 (MQ=255)

CCACTTACGTTAGTTATTAAGCAAACGTTCGCTTTTATAAACATAATCAGGATAAAAATGTTGGATTATTGCTAACCCAGCACAGCTAGTGCGCGTCTGTAATTATAAGGGAAAAACGATGAAGAATAAGGCTGATAA‑C‑AAAAAAGGAACTTCCTGACCCATAGTGAAATCGAATCACTCCTTAAAGCAGCAAATACCGGGCCTCATGCAGCACGTAATTATTGTCTGACTTTGCTTTGTTTTATTCATGGTTTCCGGGCGAGTGAAATTTGT  >  NZ_CP025268/4542754‑4543026

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

CTGGTCCACTTACGTTAGTTATTAAGCAAACGTTCGCTTTTATAAACATAATCAGGATAAAAATGTTGGATTATTGCTAACCCAGCACAGCTAGTGCGCGTCTGTAATTATAAGGGAAAAACGATGAAGAATAAGGCTGATAA‑C‑AAAAA‑AGGAACTTCCTGACCCATAGTGAAATCGAATCACTCCTTAAAGCAGCAAATACCGGGCCTCATGCAGCACGTAATTATTGTCTGACTTTGCTTTGTTTTATTCATGGTTTCCGGGCGAGTGAAATTTGTCGATTGAGGATTTCGGATAT  >  NZ_CP025268/4542749‑4543046

CTGGTCCACTTACGTTAGTTATTAAGCAAACGTTCGCTTTTATAAACATAATCAGGATAAAAATGTTGGATTATTGCTAACCCAGCACAGCTAGTGCGCGTCTGTAATTATAAGGGAAAAACGATGAAGAATAAGGCTGATAA‑C‑AAAAA‑AA                                                                                                                                                     >  SRR14920191.3386670/1‑151 (MQ=60)

  GGTCCACTTACGTTAGTTATTAAGCAAACGTTCGCTTTTATAAACATAATCAGGATAAAAATGTTGGATTATTGCTAACCCAGCACAGCTAGTGCGCGTCTGTAATTATAATGGAAAAACGATGAAGAATAAGGCTGATAA‑C‑AAAAACAGG                                                                                                                                                    >  SRR14920191.4222153/1‑151 (MQ=60)

  GGTCCACTTACGTTAGTTATTAAGCAAACGTTCGCTTTTATAAACATAATCAGGATAAAAATGTTGGATTATTGCTAACCCAGCACAGCTAGTGCGCGTCTGTAATTATAAGGGAAAAACGATGAAGAATAAGTCTGCTAA‑C‑AAAAA‑Aagg                                                                                                                                                   >  SRR14920191.185408/1‑148 (MQ=60)

  GGTCCACTTACGTTAGTTATTAAGCAAACGTTCGCTTTTATAAACATAATCAGGATAAAAATGTTGGATTATTGCTAACCCAGCACAGCTAGTGCGCGTCTGTAATTATAAGGGAAAAACGATGAAGAATAAGGCTGATAA‑C‑AAAAA‑Aagg                                                                                                                                                   >  SRR14920191.2088408/1‑148 (MQ=60)

            CGTTAGTTATTAAGCAAACGTTCGCTTTTATAAACATAATCAGGATAAAAATGTTGGATTATTGCTAACCCAGCACAGCTAGTGCGCGTCTGTAATTATAAGGGAAAAACGATGAAGAATAAGGCTGATAA‑CAAAAAA‑AGGAACTTCCTGA                                                                                                                                          >  SRR14920191.726877/1‑151 (MQ=60)

            CGTTAGTTATTAAGCAAACGTTCGCTTTTATAAACATAATCAGGATAAAAATGTTGGATTATTGCTAACCCAGCACAGCTAGTGCGCGTCTGTAATTATAAGGGAAAAACGATGAAGAATAAGGCTGATAA‑CAAAAAA‑AGGAACTTCCTGA                                                                                                                                          >  SRR14920191.726858/1‑151 (MQ=60)

             GTTAGTTATTAAGCAAACGTTCGCTTTTATAAACATAATCAGGATAAAAATGTTGGATTATTGCTAACCCAGCACAGCTAGTGCGCGTCTGTAATTATAAGGGAAAAACGATGAAGAATAAGGCTGATAA‑CAAAAAA‑AGGAACTTCCTGAC                                                                                                                                         <  SRR14920191.1847165/151‑1 (MQ=60)

              TTAGTTATTAAGCAAACGTTCGCTTTTATAAACATAATCAGGATAAAAATGTTGGATTATTGCTAACCCAGCACAGCTAGTGCGCGTCTGTAATTATAAGGGAAAAACGATGAAGAATAAGGCTGATAA‑CAAAAAA‑AGGAACTTCCTGACC                                                                                                                                        <  SRR14920191.3427246/151‑1 (MQ=60)

               TAGTTATTAAGCAAACGTTCGCTTTTATAAACATAATCAGGATAAAAATGTTGGATTATTGCTAACCCAGCACAGCTAGTGCGCGTCTGTAATTATAAGGGAAAAACGATGAAGAATAAGGCTGATAA‑CAAAAAA‑AGGAACTTCCTGACCC                                                                                                                                       <  SRR14920191.1437573/151‑1 (MQ=60)

                AGTTATTAAGCAAACGTTCGCTTTTATAAACATAATCAGGATAAAAATGTTGGATTATTGCTAACCCAGCACAGCTAGTGCGCGTCTGTAATTATAAGGGAAAAACGATGAAGAATAAGGCTGATAA‑CAAAAAA‑AGGAACTTCCTGACCCA                                                                                                                                      >  SRR14920191.2269827/1‑151 (MQ=60)

                  TTATTAAGCAAACGTTCGCTTTTATAAACATAATCAGGATAAAAATGTTGGATTATTGCTAACCCAGCACAGCTAGTGCGCGTCTGTAATTATAAGGGAAAAACGATGAAGAATAAGGCTGATAA‑CAAAAAA‑AGGAACTTCCTGACCCATA                                                                                                                                    >  SRR14920191.269113/1‑151 (MQ=60)

                  TTATTAAGCAAACGTTCGCTTTTATAAACATAATCAGGATAAAAATGTTGGATTATTGCTAACCCAGCACAGCTAGTGCGCGTCTGTAATTATAAGGGAAAAACGATGAAGAATAAGGCTGATAA‑CAAAAAA‑AGGAACTTCCTGACCCATA                                                                                                                                    >  SRR14920191.3104895/1‑151 (MQ=60)

                              CGTTCGCTTTTATAAACATAATCAGGATAAAAATGTTGGATTATTGCTAACCCAGCACAGCTAGTGCGCGTCTGTAATTATAAGGGAAAAACGATGAAGAATAAGGCTGATAA‑CAAAAAA‑AGGAACTTCCTGACCCATAGTGAAATCGAAT                                                                                                                        >  SRR14920191.3850351/1‑151 (MQ=60)

                                   GCTTTTATAAACATAATCAGGATAAAAATGTTGGATTATTGCTAACCCAGCACAGCTAGTGCGCGTCTGTAATTATAAGGGAAAAACGATGAAGAATAAGGCTGATAA‑CAAAAAA‑AGGAACTTCCTGACCCATAGTGAAATCGAATCACTC                                                                                                                   <  SRR14920191.2243781/151‑1 (MQ=60)

                                      TTTATAAACATAATCAGGATAAAAATGTTGGATTATTGCTAACCCAGCACAGCTAGTGCGCGTCTGTAATTATAAGGGAAAAACGATGAAGAATAAGGCTGATAA‑CAAAAAA‑AGGAACTTCCTGACCCATAGTGAAATCGAATCACTCCTT                                                                                                                >  SRR14920191.2900450/1‑151 (MQ=60)

                                      TTTATAAACATAATCAGGATAAAAATGTTGGATTATTGCTAACCCAGCACAGCTAGTGCGCGTCTGTAATTATAAGGGAAAAACGATGAAGAATAAGGCTGATAA‑CAAAAAA‑AGGAACTTCCTGACCCATAGTGAAATCGAATCACTCCTT                                                                                                                >  SRR14920191.4334758/1‑151 (MQ=60)

                                         ATAAACATAATCAGGATAAAAATGTTGGATTATTGCTAACCCAGCACAGCTATTGCTCGTCTGTAATTATAAGGGAAAAACGATGAAGAATAAGGCTGATAA‑CAAAAAA‑AGGAACTTCCTGACCCATAGTGAAATCGAATCACTCCTTAAA                                                                                                             <  SRR14920191.3703988/151‑1 (MQ=60)

                                           AAACATAATCAGGATAAAAATGTTGGATTATTGCTAACCCAGCACAGCTAGTGCGCGTCTGTAATTATAAGGGAAAAACGATGAAGAATAAGGCTGATAA‑CAAAAAA‑AGGAACTTCCTGACCCATAGTGAAATCGAATCACTCCTTAAAGC                                                                                                           <  SRR14920191.3154036/151‑1 (MQ=60)

                                           AAACATAATCAGGATAAAAATGTTGGATTATTGCTAACCCAGCACAGCTAGTGCGCGTCTGTAATTATAAGGGAAAAACGATGAAGAATAAGGCTGATAA‑CAAAAAA‑AGGAACTTCCTGACCCATAGTGAAATCGAATCACTCCTTAAAGC                                                                                                           <  SRR14920191.5105008/151‑1 (MQ=60)

                                           AAACATAATCAGGATAAAAATGTTGGATTATTGCTAACCCAGCACAGCTAGTGCGCGTCTGTAATTATAAGGGAAAAACGATGAAGAATAAGGCTGATAA‑CAAAAAA‑AGGAACTTCCTGACCCATAGTGAAATCGAATCACTCCTTAAAGC                                                                                                           <  SRR14920191.1432298/151‑1 (MQ=60)

                                           AAACATAATCAGGATAAAAATGTTGGATTATTGCTAACCCAGCACAGCTAGTGCGCGTCTGTAATTATAAGGGAAAAACGATGAACAATAAGGCTGATAA‑CAAAAAA‑AGGAACTTCCTGACCCATAGTGAAATCGAATCACTCCTTAAAGC                                                                                                           <  SRR14920191.4088545/151‑1 (MQ=60)

                                                      GGATAAAAATGTTGGATTATTGCTAACCCAGCACAGCTAGTGCGCGTCTGTAATTATAAGGGAAAAACGATGAAGAATAAGGCTGATAA‑CAAAAAA‑AGGAACTTCCTGACCCATAGTGAAATCGAATCACTCCTTAAAGCAGCAAATACCG                                                                                                >  SRR14920191.3996311/1‑151 (MQ=60)

                                                      GGATAAAAATGTTGGATTATTGCTAACCCAGCACAGCTAGTGCGCGTCTGTAATTATAAGGGAAAAACGATGAAGAATAAGGCTGATAA‑CAAAAAA‑AGGAACTTCCTGACCCATAGTGAAATCGAATCACTCCTTAAAGCAGCAAATACCG                                                                                                >  SRR14920191.3017196/1‑151 (MQ=60)

                                                      GGATAAAAATGTTGGATTATTGCTAACCCAGCACAGCTAGTGCGCGTCTGTAATTATAAGGGAAAAACGATGAAGAATAAGGCTGATAA‑CAAAAAA‑AGGAACTTCCTGACCCATAGTGAAATCGAATCACTCCTTAAAGCAGCAAATACCG                                                                                                >  SRR14920191.2689236/1‑151 (MQ=60)

                                                      GGATAAAAATGTTGGATTATTGCTAACCCAGCACAGCTAGTGCGCGTCTGTAATTATAAGGGAAAAACGATGAAGAATAAGGCTGATAA‑CAAAAAA‑AGGAACTTCCTGACCCATAGTGAAATCGAATCACTCCTTAAAGCAGCAAATACCG                                                                                                >  SRR14920191.3311226/1‑151 (MQ=60)

                                                         TAAAAATGTTGGATTATTGCTAACCCAGCACAGCTAGTGCGCGTCTGTAATTATAAGGGAAAAACGATGAAGAATAAGGCTGATAA‑CAAAAAA‑AGGAACTTCCTGACCCATAGTGAAATCGAATCACTCCTTAAAGCAGCAAATACCGGGC                                                                                             <  SRR14920191.2663093/151‑1 (MQ=60)

                                                         TAAAAATGTTGGATTATTGCTAACCCACCACAGCTAGTGCGCGTCTGTAATTATAAGGGAAAAACGATGAAGAATAAGGCTGATAA‑CAAAAAA‑AGGAACTTCCTGACCCATAGTGAAATCGAATCACTCCTTAAAGCAGCAAATACCGGGC                                                                                             <  SRR14920191.2663664/151‑1 (MQ=60)

                                                          AAAAATGTTGGATTATTGCTAACCCAGCACAGCTAGTGCGCGTCTGTAATTATAAGGGAAAAACGATGAAGAATAAGGCTGATAA‑CAAAAAA‑AGGAACTTCCTGACCCATAGTGAAATCGAATCACTCCTTAAAGCAGCAAATACCGGGCC                                                                                            <  SRR14920191.4101427/151‑1 (MQ=60)

                                                                GTTGGATTATTGCTAACCCAGCACAGCTAGTGCGCGTCTGTAATTATAAGGGAAAAACGATGAAGAATAAGGCTGATAA‑CAAAAAA‑AGGAACTTCCTGACCCATAGTGAAATCGAATCACTCCTTAAAGCAGCAAATACCGGGCCTCATGC                                                                                      >  SRR14920191.2816188/1‑151 (MQ=60)

                                                                GTTGGATTATTGCTAACCCAGCACAGCTAGTGCGCGTCTGTAATTATAAGGGAAAAACGATGAAGAATAAGGCTGATAA‑CAAAAAA‑AGGAACTTCCTGACCCATAGTGAAATCGAATCACTCCTTAAAGCAGCAAATACCGGGCCTCATGC                                                                                      <  SRR14920191.2841787/151‑1 (MQ=60)

                                                                GTTGGATTATTGCTAACCCAGCACAGCTAGTGCGCGTCTGTAATTATAAGGGAAAAACGATGAAGAATAAGGCTGATAA‑CAAAAAA‑AGGAACTTCCTGACCCATAGTGAAATCGAATCACTCCTTAAAGCAGCAAATACCGGGCCTCATGC                                                                                      <  SRR14920191.1542258/151‑1 (MQ=60)

                                                                GTTGGATTATTGCTAACCCAGCACAGCTAGTGCGCGTCTGTAATTATAAGGGAAAAACGATGAAGAATAAGGCTGATAA‑CAAAAAA‑AGGAACTTACTGACCCATAGTGAAATCGAATCACTCCTTAAAGCAGCAAATACCGGGCCTCATGC                                                                                      <  SRR14920191.4446095/151‑1 (MQ=60)

                                                                 TTGGATTATTGCTAACCCAGCACAGCTAGTGCGCGTCTGTAATTATAAGGGAAAAACGATGAAGAATAAGGCTGATAA‑CAAAAAA‑AGGAACTTCCTGACCCATAGTGAAATCGAATCACTCCTTAAAGCAGCAAATACCGGGCCTCATGCA                                                                                     >  SRR14920191.3401685/1‑151 (MQ=60)

                                                                 TTGGATTATTGCTAACCCAGCACAGCTAGTGCGCGTCTGTAATTATAAGGGAAAAACGATGAAGAATAAGGCTGATAA‑CAAAAAA‑AGGAACTTCCTGACCCATAGTGAAATCGAATCACTCCTTAAAGCAGCAAATACCGGGCCTCATGCA                                                                                     >  SRR14920191.765172/1‑151 (MQ=60)

                                                                   GGATTATTGCTAACCCAGCACAGCTAGTGCGCGTCTGTAATTATAAGGGAAAAACGATGAAGAATAAGGCTGATAA‑CAAAAAA‑AGGAACTTCCTGACCCATAGTGAAATCGAATCACTCCTTAAAGCAGCAAATACCGGGCCTCATGCAGC                                                                                   >  SRR14920191.873780/1‑151 (MQ=60)

                                                                   GGATTATTGCTAACCCAGCACAGCTAGTGCGCGTCTGTAATTATAAGGGAAAAACGATGAAGAATAAGGCTGATAA‑CAAAAAA‑AGGAACTTCCTGACCCATAGTGAAATCGAATCACTCC‑TAAAGCAGCAAATACCGGGCCTCATGCAGCA                                                                                  >  SRR14920191.2584712/1‑151 (MQ=60)

                                                                    GATTATTGCTAACCCAGCACAGCTAGTGCGCGTCTGTAATTATAAGGGAAAAACGATGAAGAATAAGGCTGATAA‑CAAAAAA‑AGGAACTTCCTGACCCATAGTGAAATCGAATCACTCCTTAAAGCAGCAAATACCGGGCCTCATGCAGCA                                                                                  >  SRR14920191.274420/1‑151 (MQ=60)

                                                                     ATTATTGCTAACCCAGCACAGCTAGTGCGCGTCTGTAATTATAAGGGAAAAACGATGAAGAATAAGGCTGATAA‑CAAAAAA‑AGGAACTTCCTGACCCATAGTGAAATCGAATCACTCCTTAAAGCAGCAAATACCGGGCCTCATGCAGCAC                                                                                 <  SRR14920191.4124607/151‑1 (MQ=60)

                                                                        ATTGCTAACCCAGCACAGCTAGTGCGCGTCTGTAATTATAAGGGAAAAACGATGAAGAATAAGGCTGATAA‑CAAAAAA‑AGGAACTTCCTGACCCATAGTGAAATCGAATCACTCCTTAAAGCAGCAAATACCGGGCCTCATGCAGCACGTA                                                                              >  SRR14920191.4003895/1‑151 (MQ=60)

                                                                         TTGCTAACCCAGCACAGCTAGTGCGCGTCTGTAATTATAAGGGAAAAACGATGAAGAATAAGGCTGATAA‑CAAAAAA‑AGGAACTTCCTGACCCATAGTGAAATCGAATCACTCCTTAAAGCAGCAAATACCGGGCCTCATGCAGCACGTAA                                                                             >  SRR14920191.1453222/1‑151 (MQ=60)

                                                                            CCAACCCAGCACAGCTAGTGCGCGTCTGTAATTATAAGGGAAAAACGATGAAGAATAAGGCTGATAA‑CAAAAAA‑AGGAACTTCCTGACCCATAGTGAAATCGAATCACTCCTTAAAGCAGCAAATACCGGGCCTCATGCAGCACGTAATTA                                                                          <  SRR14920191.3450929/151‑1 (MQ=60)

                                                                               ACCCAGCACAGCTAGTGCGCGTCTGTAATTATAAGGGAAAAACGATGAAGAATAAGGCTGATAA‑CAAAAAA‑AGGAACTTCCTGACCCATAGTGAAATCGAATCACTCCTTAAAGCAGCAAATACCGGGCCTCATGCAGCACGTAATTATTT                                                                       >  SRR14920191.211893/1‑151 (MQ=60)

                                                                               ACCCAGCACAGCTAGTGCGCGTCTGTAATTATAAGGGAAAAACGATGAAGAATAAGGCTGATAA‑CAAAAAA‑AGGAACTTCCTGACCCATAGTGAAATCGAATCACTCCTTAAAGCAGCAAATACCGGGCCTCATGCAGCACGTAATTATTG                                                                       >  SRR14920191.1751180/1‑151 (MQ=60)

                                                                               ACCCAGCACAGCTAGTGCGCGTCTGTAATTATAAGGGAAAAACGATGAAGAATAAGGCTGATAA‑CAAAAAA‑AGGAACTTCCTGACCCATAGTGAAATCGAATCACTCCTTAAAGCAGCAAATACCGGGCCTCATGCAGCACGTAATTATTG                                                                       >  SRR14920191.709187/1‑151 (MQ=60)

                                                                               ACCCAGCACAGCTAGTGCGCGTCTGTAATTATAAGGGAAAAACGATGAAGAATAAGGCTGATAA‑CAAAAAA‑AGGAACTTCCTGACCCATAGTGAAATCGAATCACTCCTTAAAGCAGCAAATACCGGGCCTCATGCAGCACGTAATTATTG                                                                       <  SRR14920191.2051548/151‑1 (MQ=60)

                                                                               ACCCAGCACAGCTAGTGCGCGTCTGTAATTATAAGGGAAAAACGATGAAGAATAAGGCTGATAA‑CAAAAAA‑AGGAACTTCCTGACCCATAGTGAAATCGAATCACTCCTTAAAGCAGCAAATACCGGGCCTCATGCAGCACGTAATTATTG                                                                       >  SRR14920191.4256139/1‑151 (MQ=60)

                                                                               ACCCAGCACAGCTAGTGCGCGTCTGTAATTATAAGGGAAAAACGATGAAGAATAAGGCTGATAA‑CAAAAAA‑AGGAACTTCCTGACCCATAGTGAAATCGAATCACTCCTTAAAGCAGCAAATACCGGGCCTCATGCAGCACGTAATTATTG                                                                       >  SRR14920191.1736898/1‑151 (MQ=60)

                                                                               ACCCAGCACAGCTAGTGCGCGTCTGTAATTATAAGGGAAAAACGATGAAGAATAAGGCTGATAA‑CAAAAAA‑AGGAACTTCCTGACCCATAGTGAAATCGAATCACTCCTTAAAGCAGCAAATACCGGGCCTCATGCAGCACGTAATTATTG                                                                       >  SRR14920191.211927/1‑151 (MQ=60)

                                                                               ACCCAGCACAGCTAGTGCGCGTCTGTAATTATAAGGGAAAAACGATGAAGAATAAGGCTGATAA‑CAAAAAA‑AGGAACTTCCTGACCCATAGTGAAATCGAATCACTCCTTAAAGCAGCAAATACCGGGCCTCATGCAGCACGTAATTATTG                                                                       >  SRR14920191.3529396/1‑151 (MQ=60)

                                                                               ACCCAGCACAGCTAGTGCGCGTCTGTAATTATAAGGGAAAAACGATGAAGAATAAGGCTGATAA‑CAAAAAA‑AGGAACTTCCTGACCCATAGTGAAATCGAATCACTCCTTAAAGCAGCAAATACCGGGCCTCATGCAGCACGTAATTATTG                                                                       >  SRR14920191.2802131/1‑151 (MQ=60)

                                                                                   AGCACAGCTAGTGCGCGTCTGTAATTATAAGGGAAAAACGATGAAGAATAAGGCTGATAA‑CAAAAAA‑AGGAACTTCCTGACCCATAGTGAAATCGAATCACTCCTTAAAGCAGCAAATACCGGGCCTCATGCAGCACGTAATTATTGTCTG                                                                   >  SRR14920191.835381/1‑151 (MQ=60)

                                                                                       CAGCTAGTGCGCGTCTGTAATTATAAGGGAAAAACGATGAAGAATAAGGCTGATAA‑CAAAAAA‑AGGAACTTCCTGACCCATAGTGAAATCGAATCACTCCTTAAAGCAGCAAATACCGGGCCTCATGCAGCACGTAATTATTGTCTGACTT                                                               <  SRR14920191.1889263/151‑1 (MQ=60)

                                                                                       CAGCTAGTGCGCGTCTGTAATTATAAGGGAAAAACGATGAAGAATAAGGCTGATAA‑CAAAAAA‑AGGAACTTCCTGACCCATAGTGAAATCGAATCACTCCTTAAAGCAGCAAATACCGGGCCTCATGCAGCACGTAATTATTGTCTGACTT                                                               >  SRR14920191.4715110/1‑151 (MQ=60)

                                                                                       CAGCTAGTGCGCGTCTGTAATTATAAGGGAAAAACGATGAAGAATAAGGCTGATAA‑CAAAAAA‑AGGAACTTCCTGACCCATAGTGAAATCGAATCACTCCTTAAAGCAGCAAATACCGGGCCTCATGCAGCACGTAATTATTGTCTGACTT                                                               <  SRR14920191.1888959/151‑1 (MQ=60)

                                                                                         GCTAGTGCGCGTCTGTAATTATAAGGGAAAAACGATGAAGAATAAGGCTGATAA‑CAAAAAA‑AGGAACTTCCTGACCCATAGTGAAATCGAATCACTCCTTAAAGCAGCAAATACCGGGCCTCATGCAGCACGTAATTATTGTCTGACTTTG                                                             >  SRR14920191.1629002/1‑151 (MQ=60)

                                                                                         GCTAGTGCGCGTCTGTAATTATAAGGGAAAAACGATGAAGAATAAGGCTGATAA‑CAAAAAA‑AGGAACTTCCTGACCCATAGTGAAATCGAATCACTCCTTAAAGCAGCAAATACCGGGCCTCATGCAGCACGTAATTATTGTCTGACTTTG                                                             >  SRR14920191.1596528/1‑151 (MQ=60)

                                                                                         GCTAGTGCGCGTCTGTAATTATAAGGGAAAAACGATGAAGAATAAGGCTGATAA‑CAAAAAA‑AGGAACTTCCTGACCCATAGTGAAATCGAATCACTCCTTAAAGCAGCAAATACCGGGCCTCATGCAGCACGTAATTATTGTCTGACTTTG                                                             >  SRR14920191.4676305/1‑151 (MQ=60)

                                                                                                  CGTCTGTAATTATAAGGGAAAAACGATGAAGAATAAGGCTGATAA‑CAAAAAA‑AGGAACTTCCTGACCCATAGTGAAATCGAATCACTCCTTAAAGCAGCAAATACCGGGCCTCATGCAGCACGTAATTATTGTCTGACTTTGCTTTGTTTT                                                    >  SRR14920191.3908953/1‑151 (MQ=60)

                                                                                                    TCTGTAATTATAAGGGAAAAACGATGAAGAATAAGGCTGATAA‑CAAAAAA‑AGGAACTTCCTGACCCATAGTGAAATCGAATCACTCCTTAAAGCAGCAAATACCGGGCCTCATGCAGCACGTAATTATTGTCTGACTTTGCTTTGTTTTAT                                                  >  SRR14920191.639613/1‑151 (MQ=60)

                                                                                                      TGTAATTATAAGGGAAAAACGATGAAGAATAAGGCTGATAA‑CAAAAAA‑AGGAACTTCCTGACCCATAGTGAAATCGAATCACTCCTTAAAGCAGCAAATACCGGGCCTCATGCAGCACGTAATTATTGTCTGACTTTGCTTTGTTTTATTC                                                <  SRR14920191.3519345/151‑1 (MQ=60)

                                                                                                      TGTAATTATAAGGGAAAAACGATGAAGAATAAGGCTGATAA‑CAAAAAA‑AGGAACTTCCTGACCCATAGTGAAATCGAATCACTCCTTAAAGCAGCAAATACCGGGCCTCATGCAGCACGTAATTATTGTCTGACTTTGCTTTGTTTTATTC                                                <  SRR14920191.1678448/151‑1 (MQ=60)

                                                                                                       GTAATTATAAGGGAAAAACGATGAAGAATAAGGCTGATAA‑CAAAAAA‑AGGAACTTCCTGACCCATAGTGAAATCGAATCACTCCTTAAAGCAGCAAATACCGGGCCTCATGCAGCACGTAATTATTGTCTGACTTTGCTTTGTTTTATTCA                                               >  SRR14920191.4874289/1‑151 (MQ=60)

                                                                                                           TTATAAGGGAAAAACGATGAAGAATGAGGCTGATAA‑CAAAAAA‑AGGAACTTCCTGACCCATAGTGAAATCGAATCACTCCTTAAAGCAGCAAATACCGGGCCTCATGCAGCACGTAATTATTGTCTGACTTTGCTTTGTTTTATTCATGGT                                           >  SRR14920191.458715/1‑151 (MQ=60)

                                                                                                           TTATAAGGGAAAAACGATGAAGAATAAGGCTGATAA‑CAAAAAA‑AGGAACTTCCTGACCCATAGTGAAATCGAATCACTCCTTAAAGCAGCAAATACCGGGCCTCATGCAGCACGTAATTATTGTCTGACTTTGCTTTGTTTTATTCATGGT                                           >  SRR14920191.3428378/1‑151 (MQ=60)

                                                                                                              TAAGGGAAAAACGATGAAGAATAAGGCTGATAACC‑AAAAA‑AGGAACTTCCTGACCCATCGTGAAATCGAATCACTCCTTAAAGCAGCAAATACCGGGCCTCATGCAGCACGTAATTATTGTCTGACTTTGCTTTGTTTTATTCATGGTTTC                                        <  SRR14920191.4320051/151‑1 (MQ=60)

                                                                                                                  GGAAAAACGATGAAGAATAAGGCTGATAA‑CAAAAAA‑AGGAACTTCCTGACCCATAGTGAAATCGAATCACTCCTTAAAGCAGCAAATACCGGGCCTCATGCAGCACGTAATTATTGTCTGACTTTGCTTTGTTTTATTCATGGTTTCCGGG                                    <  SRR14920191.4898539/151‑1 (MQ=60)

                                                                                                                   GAAAAACGATGAAGAATAAGGCTGATAA‑CAAAAAA‑AGGAACTTCCTGACCCATAGTGAAATCGAATCACTCCTTAAAGCAGCAAATACCGGGCCTCATGCAGCACGTAATTATTGTCTGACTTTGCTTTGTTTTATTCATGGTTTCCGGGC                                   >  SRR14920191.865268/1‑151 (MQ=60)

                                                                                                                    AAAAACGATGAAGAATAAGGCTGATAA‑CAAAAAA‑AGGAACCTCCTGACCCATAGTGAAATCGAATCACTCCTTAAAGCAGCAAATACCGGGCCTCATGCAGCACGTAATTATTGTCTGACTTTGCTTTGTTTTATTCATGGTTTCCGGGCG                                  <  SRR14920191.4789222/151‑1 (MQ=60)

                                                                                                                                GAATAAGGCTGATAA‑CAAAAAA‑AGGAACTTCCTGACCCATAGTGAAATCGAATCACTCCTTAAAGCAGCAAATACCGGGCCTCATGCAGCACGTAATTATTGTCTGACTTTGCTTTGTTTTATTCATGGTTTCCGGGCGAGTGAAATTTGT                      >  SRR14920191.4861606/1‑151 (MQ=60)

                                                                                                                                GAATAAGGCTGATAA‑CAAAAAA‑AGGAACTTCCTGACCCATAGTGAAATCGAATCACTCCTTAAAGCAGCAAATACCGGGCCTCATGCAGCACGTAATTATTGTCTGACTTTGCTTTGTTTTATTCATGGTTTCCGGGCGAGTGAAATTTGT                      >  SRR14920191.3136548/1‑151 (MQ=60)

                                                                                                                                 AATAAGGCTGATAA‑CAAAAAA‑AGGAACTTCCTGACCCATAGTGAAATCGAATCACTCCTTAAAGCAGCAAATACCGGGCCTCATGCAGCACGTAATTATTGTCTGACTTTGCTTTGTTTTATTCATGGTTTCCGGGCGAGTGAAATTTGTC                     >  SRR14920191.57327/1‑151 (MQ=60)

                                                                                                                                           ATAA‑CAAAAAA‑AGGAACTTCCTGACCCATAGTGAAATCGAATCACTCCTTAAAGCAGCAAATACCGGGCCTCATGCAGCACGTAATTATTGTCTGACTTTGCTTTGTTTTATTCATGGTTTCCGGGCGAGTGAAATTTGTCGATTGAGGAT           <  SRR14920191.1471706/151‑1 (MQ=60)

                                                                                                                                                caAAAAA‑AGGAACTTCCTGACCCATAGTGAAATCGAATCACTCCTTAAAGCAGCAAATACCGGGCCTCATGCAGCACGTAATTATTGTCTGACTTTGCTTTGTTTTATTCATGGTTTCCGGGCGAGGGAAATTTGTCGATTGAGGATTTCG       >  SRR14920191.569704/3‑151 (MQ=60)

                                                                                                                                                    AAA‑AGGAACTTCCTGACCCATAGTGAAATCGAATCACTCCTTAAAGCAGCAAATACCGGGCCTCATGCAGCACGTAATTATTGTCTGACTTTGCTTTGTTTTATTCATGGTTTCCGGGCGAGTGAAATTTGTCGATTGAGGATTTCGGATA   >  SRR14920191.690325/1‑151 (MQ=60)

                                                                                                                                                     AA‑AGGAACTTCCTGACCCATAGTGAAATCGAATCACTCCTTAAAGCAGCAAATACCGGGCCTCATGCAGCACGTAATTATTGTCTGACTTTGCTTTGTTTTATTCATGGTTTCCGGGCGAGTGAAATTTGTCGATTGAGGATTTCGGATAT  <  SRR14920191.4813448/151‑1 (MQ=60)

CTGGTCCACTTACGTTAGTTATTAAGCAAACGTTCGCTTTTATAAACATAATCAGGATAAAAATGTTGGATTATTGCTAACCCAGCACAGCTAGTGCGCGTCTGTAATTATAAGGGAAAAACGATGAAGAATAAGGCTGATAA‑C‑AAAAA‑AGGAACTTCCTGACCCATAGTGAAATCGAATCACTCCTTAAAGCAGCAAATACCGGGCCTCATGCAGCACGTAATTATTGTCTGACTTTGCTTTGTTTTATTCATGGTTTCCGGGCGAGTGAAATTTGTCGATTGAGGATTTCGGATAT  >  NZ_CP025268/4542749‑4543046

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`