Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F50 I1 R2	870	341.9	11209456	97.2%	10895591	138.9

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	1,629,176	(T)_7→8	pseudogene (141/392 nt)	ydeI ←	YdeI family stress tolerance OB fold protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	1,629,169	1	.	T	97.0%	490.7 / 5.8	137	pseudogene (148/392 nt)	ydeI	YdeI family stress tolerance OB fold protein
Reads supporting (aligned to +/- strand): ref base . (0/4); new base T (29/102); total (30/107)
Fisher's exact test for biased strand distribution p-value = 5.77e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 144 total aligned reads displayed.

GCCGCAGGAATAACGACATTAATTTCACCGCTCTTATCGCGAAAAACGTAACGGTCCTCTCCTTTGTGAGAAATCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCA‑T‑TTT‑TT‑TGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATCCTGTTTTAAACCGCCCTGGTCATCTGCCAACGCATAAGGCATGACAAGAAAACTTGCTAATACAATG  >  NZ_CP025268/1629034‑1629302

gcggCAGGAATAACGACATTAATTTCACCGCTCTTATCGCGAAAAACGTAACGGTCCTCTCCGTTGTGAGAAATCAAATTACCGCGTAGTGAAACCGAAGCGTCATCGTGGAGGGTTTTTGCGAAATCAACGGTCA‑t‑ttt                                                                                                                                     >  2:411110/4‑140 (MQ=255)

gCCGCAGGAATAACGACATTAATTTCACCGCTCTTATCGCGAAAAACGTAACGGTCCTCTCCTTTGTGAGAAATCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCA‑t‑ttt                                                                                                                                     >  1:380789/1‑140 (MQ=255)

gCCGCAGGAATAACGACATTAATTTCACCGCTCTTATCGCGAAAAACGTAACGGTCCTCTCCTTTGTGAGAAATCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCA‑t‑ttt                                                                                                                                     >  1:5459931/1‑140 (MQ=255)

gCCGCAGGAATAACGACATTAATTTCACCGCTCTTATCGCGAAAAACGTAACGGTCCTCTCCTTTGTGAGAAATCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCA‑t‑ttt                                                                                                                                     >  2:2611189/1‑140 (MQ=255)

gCCGCAGGAATAACGACATTAATTTCACCGCTCTTATCGCGAAAAACGTAACGGTCCTCTCCTTTGTGAGAAATCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCA‑t‑ttt                                                                                                                                     >  1:4321158/1‑140 (MQ=255)

   gCAGGAATAACGACATTAATTTCACCGCTCTTATCGCGAAAAACGTAACGGTCCTCTCCTTTGTGAGAAATCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCA‑t‑ttt‑tt‑t                                                                                                                                <  2:296874/140‑1 (MQ=255)

      ggAATAACGACATTAATTTCACCGCTCTTATCGCGAAAAACGTAACGGTCCTCTCCTTTGTGAGAAATCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCA‑t‑ttt‑tt‑ttgc                                                                                                                             <  1:4760172/140‑4 (MQ=255)

           aaCGACATTAATTTCACCGCTCTTATCGCGAAAAACGTAACGGTCCTCTCCTTTGTGAGAAATCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGAATGGTGTTTGCGAAATCAACGGTCATT‑TTT‑TT‑TGcatcat                                                                                                                         <  2:1750994/140‑1 (MQ=255)

             cGAGATTAATTTCACCGCTCTTATCGCGAAAAACGTAACGGTCCTCTCCTTTGTGAGAAATCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCATT‑TTT‑TT‑TGCATCATCg                                                                                                                       >  1:3560903/1‑140 (MQ=255)

               aCATTAATTTCACCGCTCTTATCGCGAAAAACGTAACGGTCCTCTCCTTTGTGAGAAATCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCATT‑TTT‑TT‑TGCATCATCGGt                                                                                                                     <  1:2652129/140‑1 (MQ=255)

                 aTTAATTTCCCCGCTCTTATCGCGAAAAACGTAACGGTCCTCTCCTTTGTGAGAAATCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCATT‑TTT‑TT‑TGCATCATCGGTTc                                                                                                                   <  2:3856402/140‑1 (MQ=255)

                 aTTAATTTCACCGCTCTTATCGCGAAAAACGTAACGGTCCTCTCCTTTGTGAGAAATCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCATT‑TTT‑TT‑TGCATCATCGGTTc                                                                                                                   >  2:4062417/1‑140 (MQ=255)

                  ttAATTTCACCGCTCTTATCGCGAAAAACGTAACGGTCCTCTCCTTTGTGAGAAATCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCATT‑TTT‑TT‑TGCATCATCGGTTcc                                                                                                                  >  2:4513961/1‑140 (MQ=255)

                   tAATTTCACCGCTCTTATCGCGAAAAACGTAACGGTCCTCTCCTTTGTGAGAAATCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCATT‑TTT‑TT‑TGCATCATCGGTTCcg                                                                                                                 <  1:4342407/140‑1 (MQ=255)

                     aTTTCACCGCTCTTATCGCGAAAAACGTAACGGTCCTCTCCTTTGTGAGAAATCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCATT‑TTT‑TT‑TGCATCATCGGTTCcgcg                                                                                                               >  2:2248102/1‑140 (MQ=255)

                       ttCACCGCTCTTATCGCGAAAAACGTAACGGTCCTCTCCTTTGTGAGAAATCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCATT‑TTT‑TT‑TGCATCATCGGTTCCGCGAt                                                                                                             <  1:5179350/140‑1 (MQ=255)

                         cACCGCTCTTATCGCGAAAAACGTAACGGTCCTCTCCTTTGTGAGAAATCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCATT‑TTT‑TT‑TGCATCATCGGTTCCGCGATAg                                                                                                           <  1:169482/140‑1 (MQ=255)

                           ccGCTCTTGTCGCGAAAAGCGTAACGGTCCGCTCCTTTGGGCGGAATCAAATTACCGCGTAGTGAACCCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCA‑T‑TTT‑TTGTGCATCATCGGTTCCGCGATAGcc                                                                                                         <  2:1040768/140‑1 (MQ=255)

                           ccGCTCTTATCGCGAAAAACGTAACGGTCCTCTCCTTTGTGAGAAATCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCATT‑TTT‑TT‑TGCATCATCGGTTCCGCGATAGcc                                                                                                         <  2:1293129/140‑1 (MQ=255)

                           ccGCTCTTATCGCGAAAAACGTAACGGTCCTCTCCTTTGTGAGAAATCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCATT‑TTT‑TT‑TGCATCATCGGTTCCGCGATAGcc                                                                                                         <  1:4955274/140‑1 (MQ=255)

                           ccGCTCTTATCGCGAAAAACGTAACGGTCCTCTCCTTTGTGAGAAATCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCATT‑TTT‑TT‑TGCATCATCGGTTCCGCGATAGcc                                                                                                         <  1:945279/140‑1 (MQ=255)

                           ccGCTCTTATCGCGAAAAACGTAACGGTCCTCTCCTTTGTGAGAAATCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCATT‑TTT‑TT‑TGCATCATCGGTTCCGCGATAGcc                                                                                                         <  1:1065162/140‑1 (MQ=255)

                           ccGCTCTTATCGCGAAAAACGTAACGGTCCTCTCCTTTGTGAGAAATCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCATT‑TTT‑TT‑TGCATCATCGGTTCCGCGATAGcc                                                                                                         <  2:628610/140‑1 (MQ=255)

                           ccGCTCTTATCGCGAAAAACGTAACGGTCCTCTCCTTTGTGAGAAATCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCATT‑TTT‑TT‑TGCATCATCGGTTCCGCGATAGcc                                                                                                         <  1:3291060/140‑1 (MQ=255)

                           ccGCTCTTATCGCGAAAAACGTAACGGTCCTCTCCTTTGTGAGAAATCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCATT‑TTT‑TT‑TGCATCATCGGTTCCGCGATAGcc                                                                                                         >  2:4955274/1‑140 (MQ=255)

                           ccGCTCTTACCGCGAAAACCGTAACGGTCCTCTCCTTTGTGAGAAATCAAATTACCGCGTAGTGAAACCGAAGCGCCCTCGTGCATGGTTTTTGCGAAATCAACGGTCATT‑TTT‑TT‑TGCATCATCGGTTCCGCGATAGcc                                                                                                         <  2:4897027/140‑1 (MQ=255)

                            cGCTCTTATCGCGAAAAACGTAACGGTCCTCTCCTTTGTGAGAAATCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCATT‑TTT‑TT‑TGCATCATCGGTTCCGCGATAGCCa                                                                                                        <  2:4164024/140‑1 (MQ=255)

                             gCTCTTATCGCGAAAAACGTAACGGTCCTCTCCTTTGTGAGAAATCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCATT‑TTT‑TT‑TGCATCATCGGTTCCGCGATAGCCAt                                                                                                       <  1:2281799/140‑1 (MQ=255)

                              ctctTATCGCGCAAAACGTAACGGTCCGCGCCTTTGTGAGAAATCAAATTACCGCGTAGTGAAACCGAAGCCCCATCGGGCCTGGTTGTTGCGAAATCAACGGTCACT‑TTT‑TT‑TGCATCATCGGTTCCGCGATAGCCAtc                                                                                                      <  1:4502326/140‑1 (MQ=255)

                              ctctTATCGCGAAAAACGTAACGGTCCTCTCCTTTGTGAGAAATCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCATT‑TTT‑TT‑TGCATCATCGGTTCCGCGATAGCCAtc                                                                                                      >  2:3822106/1‑140 (MQ=255)

                              ctctTATCGCGAAAAACGTAACGGTCCTCTCCTTTGTGAGAAATCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCATT‑TTT‑TT‑TGCATCATCGGTTCCGCGATAGCCAtc                                                                                                      >  1:2052657/1‑140 (MQ=255)

                                ctTATCGCGAAAAACGTAACGGTCCTCTCCTTTGTGAGAAATCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCATT‑TTT‑TT‑TGCATCATCGGTTCCGCGATAGCCAtctt                                                                                                    <  1:3812811/140‑1 (MQ=255)

                                  tATCGCGAAAACCGTAACGGTCCACTCCTTTGTGAGAAATCAAATTACCGCGTAGTGAATCCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCATT‑TTT‑TT‑TGCATCATCGGTTCCGCGATAGCCAtcttct                                                                                                  <  1:5069256/140‑1 (MQ=255)

                                   aTCGCGAAAAACGTAACGGTCCTCTCCTTTGTGAGAAATCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCATT‑TTT‑TT‑TGCATCATCGGTTCCGCGATAGCCATCTTCTa                                                                                                 <  2:2728286/140‑1 (MQ=255)

                                   aTCGCGAAAAACGTAACGGTCCTCTCCTTTGTGAGAAATCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCATT‑TTT‑TT‑TGCATCATCGGTTCCGCGATAGCCATCTTCTa                                                                                                 >  1:1879803/1‑140 (MQ=255)

                                        gaacaccGTAACGGTCCTCTCCTTTGTGCGAAATCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCATT‑TTT‑TT‑TGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCa                                                                                            <  2:5066862/134‑1 (MQ=255)

                                        gAAAAACGTAACGGTCCTCTCCTTTGTGAGAAATCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCATT‑TTT‑TT‑TGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCa                                                                                            <  1:3842391/140‑1 (MQ=255)

                                        gAAAAACGTAACGGACCTCTCCTTTGTGAGAAATCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCATT‑TTT‑TT‑TGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCa                                                                                            <  2:4593867/140‑1 (MQ=255)

                                         aaaaaCGTAACGGTCCTCTCCTTTGTGAGAAATCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCATT‑TTT‑TT‑TGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCAt                                                                                           >  1:3132821/1‑140 (MQ=255)

                                         aaaaaCGTAACGGTCCTCTCCTTTGTGAGAAATCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCATT‑TTT‑TT‑TGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCAt                                                                                           <  1:4792573/140‑1 (MQ=255)

                                           aaaCGTAACGGTCCTCTCCTTTGTGAGAAATCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCATT‑TTT‑TT‑TGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGa                                                                                         >  1:1124110/1‑140 (MQ=255)

                                                tAACGGTCCTCTCCTTTGTGAGAAATCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCATT‑TTT‑TT‑TGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAggcgg                                                                                    <  1:2268472/140‑1 (MQ=255)

                                                tAACGGTCCTCTCCTTTGTGAGAAATCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCATT‑TTT‑TT‑TGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAggcgg                                                                                    <  1:3922229/140‑1 (MQ=255)

                                                   cGGTCCTCTCCTTTGTGAGAAATCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCATT‑TTT‑TT‑TGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGcggcgg                                                                                 <  1:4129718/140‑1 (MQ=255)

                                                      tCCTCTCCTTTGTGAGAAATCAAATTACCGCGTAGTGAAACCGAAGCGCTATCGTGCATGGTTTTTGCGAAATCAACGGTCATT‑TTT‑TT‑TGCATCATCGGTTCCGCAATAGCCATCTTCTATTGCATGAGGCGGCGGTgg                                                                              >  2:2701563/1‑140 (MQ=255)

                                                        ctctCCTTTGTGAGAAATCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCATT‑TTT‑TT‑TGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGgcg                                                                            >  2:4288220/1‑140 (MQ=255)

                                                        ctctCCTTTGTGAGAAATAAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCATT‑TTT‑TT‑TGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGgcg                                                                            >  2:4885596/1‑140 (MQ=255)

                                                               ttGTGAGAAATCAAATTTCCGCGTCGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCATT‑TTT‑TT‑TGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATc                                                                     <  1:3277621/140‑1 (MQ=255)

                                                               ttGTGAGAAATCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCATT‑TTT‑TT‑TGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATc                                                                     <  2:181820/140‑1 (MQ=255)

                                                               ttGTGAGAAATCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCATT‑TTT‑TT‑TGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATc                                                                     <  2:4511145/140‑1 (MQ=255)

                                                               ttGTGAGAAATCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCATT‑TTT‑TT‑TGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATc                                                                     <  1:862258/140‑1 (MQ=255)

                                                               ttGTGAGAAATCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCATT‑TTT‑TT‑TGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATc                                                                     <  1:1443089/140‑1 (MQ=255)

                                                                tgtgAGAAATCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGTTCATT‑TTT‑TT‑TGCATCATCGGTTCCGCGATAGCCAGCTTCTATTGCATGATGCGGCGGTGGCGCTGTATcc                                                                    >  2:4328430/1‑140 (MQ=255)

                                                                tgtgAGAAATCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCATT‑TTT‑TT‑TGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATcc                                                                    >  1:5574737/1‑140 (MQ=255)

                                                                tgtgAGAAATCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCATT‑TTT‑TT‑TGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATcc                                                                    <  1:3721184/140‑1 (MQ=255)

                                                                        aTCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCATT‑TTT‑TT‑TGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATCCTGTTTTaa                                                            <  2:4616824/140‑1 (MQ=255)

                                                                         tCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCATT‑TTT‑TT‑TGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATCCTGTTTTaaa                                                           <  1:4609522/140‑1 (MQ=255)

                                                                         tCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCATT‑TTT‑TT‑TGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATCCTGTTTTaaa                                                           <  1:426009/140‑1 (MQ=255)

                                                                          cAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCATT‑TTT‑TT‑TGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATCCTGTTTTAAAc                                                          <  2:3501333/140‑1 (MQ=255)

                                                                             aTTACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCATT‑TTT‑TT‑TGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATCCTGTTTTAAACCGc                                                       <  2:336072/140‑1 (MQ=255)

                                                                              ttcgcgcgTAGTGAAACCGACGCGCCACCGTGCATGGTTTTTGCGAAATCAACGGTCA‑T‑TTTGTT‑TGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATCCTGTTTTAAACCGcc                                                      <  2:3837562/136‑1 (MQ=255)

                                                                              ttACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCATT‑TTT‑TT‑TGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATCCTGTTTTAAACCGcc                                                      <  1:2980502/140‑1 (MQ=255)

                                                                                aCCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCATT‑TTT‑TT‑TGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATCCTGTTTTAAACCGCCCt                                                    <  2:2478185/140‑1 (MQ=255)

                                                                                aCCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCATT‑TTT‑TT‑TGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATCCTGTTTTAAACCGCCCt                                                    >  1:4705115/1‑140 (MQ=255)

                                                                                   gcgTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCATT‑TTT‑TT‑TGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATCCTGTTTTAAACCGCCCTGGt                                                 >  2:2159517/1‑140 (MQ=255)

                                                                                    cgTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCATT‑TTT‑TT‑TGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATCCTGTTTTAAACCGCCCTGGTc                                                >  1:4114273/1‑140 (MQ=255)

                                                                                           aaaCCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCATT‑TTT‑TT‑TGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATCCTGTTTTAAACCGCCCTGGTCATCTGcc                                         <  1:4045458/140‑1 (MQ=255)

                                                                                           aaaCCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCATT‑TTT‑TT‑TGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATCCTGTTTTAAACCGCCCTGGTCATCTGcc                                         <  1:1852053/140‑1 (MQ=255)

                                                                                            aaCCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCATT‑TTT‑TT‑TGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATCCTGTTTTAAACCGCCCTGGTCATCTGCCa                                        <  1:5219127/140‑1 (MQ=255)

                                                                                            aaCCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCATT‑TTT‑TT‑TGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATCCTGTTTTAAACCGCCCTGGTCATCTGCCa                                        <  1:4014726/140‑1 (MQ=255)

                                                                                            aaCCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCATT‑TTT‑TT‑TGCATCATCGGGTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATCCTGTTTTAAACCGCCCTGGTCATCTGCCa                                        <  2:310914/140‑1 (MQ=255)

                                                                                             aCCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCATT‑TTT‑TT‑TGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATCCTGTTTTAAACCGCCCTGGTCATCTGCCaa                                       <  2:3467418/140‑1 (MQ=255)

                                                                                                 aaGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCATT‑TTT‑TT‑TGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATCCTGTTTTAAACCGCCCTGGTCATCTGCCAACGCa                                   <  1:2611189/140‑1 (MQ=255)

                                                                                                 aaGCGCCATCGTGCATGGTTTTTGCAAAATCAACGGTCATT‑TTT‑TT‑TGCATCCTCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATACTCTTTTAAACCGCCCTGGTCATCTGCCAACGCa                                   <  2:3593245/140‑1 (MQ=255)

                                                                                                   gcgcCATCGTGCATGGTTTTTGCGAAATCAACGGTCATT‑TTT‑TT‑TGCGTCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATCCTGTTTTAAACCGCCCTGGTCATCTGCCAACGCATa                                 >  2:4376896/1‑140 (MQ=255)

                                                                                                         tCGTTCATGGTTTTTGCGAAATCAACGGTTATT‑TTT‑TT‑TGCATCATCGGTTTCGCTATAGCCATCTTCTATTGCATGAGGCTGCGGTGGCGCTGCATCCTGTTTTAAACCGCCCTGGTCATCTGCCAACGCATAAGGCAt                           <  2:1923143/140‑1 (MQ=255)

                                                                                                         tCGTGCATGGTTTTTGCGAAATCAACGGTCATT‑TTT‑TT‑TGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATCCTGTTTTAAACCGCCCTGGTCATCTGCCAACGCATAAGGCAt                           <  2:508013/140‑1 (MQ=255)

                                                                                                         tCGTGCATGGTTTTTGCGAAATCAACGGTCA‑TGTTT‑TT‑TGCAGCATCGGTTCCGCGATAGCCATCCTCTATTGCATGAGGCGGCGGTGGCGCTGCATCCTGTTTTAAACCGCCCTGGGCATCTCCCAACGCATAAGGCAt                           <  2:4962726/140‑1 (MQ=255)

                                                                                                          cGTGCATGGTTTTTGCGAAATCAACGGTCATT‑TTT‑TT‑TGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATCCTGTTTTAAACCGCCCTGGTCATCTGCCAACGCATAAGGCATg                          <  1:1420178/140‑1 (MQ=255)

                                                                                                            tGCATGGTTTTTGCGAAATCAACGGTCATT‑TTT‑TT‑TGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATCCTGTTTTAAACCGCCCTGGTCATCTGCCAACGCATAAGGCATGAc                        >  1:2421654/1‑140 (MQ=255)

                                                                                                              cATGGTTTTTGCGAAATCAACGGTCATT‑TTT‑TT‑TGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATCCTGTTTTAAACCGCCCTGGTCATCTGCCAACGCATAAGGCATGACaa                      >  1:1077103/1‑140 (MQ=255)

                                                                                                                tGGTTTTTGCGAAATCAACGGTCATT‑TTT‑TT‑TGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATCCTGTTTTAAACCGCCCTGGTCATCTGCCAACGCATAAGGCATGACAAGa                    <  1:1551576/140‑1 (MQ=255)

                                                                                                                 ggTTTTTGCGAAATCAACGGTCATT‑TTT‑TT‑TGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATCCTGTTTTAAACCGCCCTGGTCATCTGCCAACGCATAAGGCATGACAAGaa                   <  2:3056612/140‑1 (MQ=255)

                                                                                                                   tttttGCGATATCAACGGTCATT‑TTT‑TT‑TGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATCCTGTTTTAAACCGCCCTGGTCATCTGCCAACGCATAAGGCATGACAAGaaaa                 <  2:2638592/140‑1 (MQ=255)

                                                                                                                   tttttGCGAAATCAACGGTCATT‑TTT‑TT‑TGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATCCTGTTTTAAACCGCCCTGGTCATCTGCCAACGCATAAGGCATGACAAGaaaa                 <  2:4321158/140‑1 (MQ=255)

                                                                                                                   tttttGCGAAATCAACGGTCATT‑TTT‑TT‑TGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATCCTGTTTTAAACCGCCCTGGTCATCTGCCAACGCATAAGGCATGACAAGaaaa                 <  2:5495639/140‑1 (MQ=255)

                                                                                                                   tttttGCGAAATCAACGGTCATT‑TTT‑TT‑TGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATCCTGTTTTAAACCGCCCTGGTCATCTGCCAACGCATAAGGCATGACAAGaaaa                 <  1:1556589/140‑1 (MQ=255)

                                                                                                                   tttttGCGAAATCAACGGTCATT‑TTT‑TT‑TGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATCCTGTTTTAAACCGCCCTGGTCATCTGCCAACGCATAAGGCATGACAAGaaaa                 <  2:4694591/140‑1 (MQ=255)

                                                                                                                   tttttGCGAAATCAACGGTCATT‑TTT‑TT‑TGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATCCTGTTTTAAACCGCCCTGGTCATCTGCCAACGCATAAGGCATGACAAGaaaa                 <  1:4513961/140‑1 (MQ=255)

                                                                                                                   tttttGCGAAATCAACGGTCATT‑TTT‑TT‑TGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATCCTGTTTTAAACCGCCCTGGTCATCTGCCAACGCATAAGGCATGACAAGaaaa                 <  2:2052657/140‑1 (MQ=255)

                                                                                                                   tttttGCGAAATCAACGGTCATT‑TTT‑TT‑TGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATCCTGTTTTAAACCGCCCTGGTCATCTGCCAACGCATAAGGCATGACAAGaaaa                 <  1:2737668/140‑1 (MQ=255)

                                                                                                                   tttttGCGAAATCAACGGTCATT‑TTT‑TT‑TGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATACTGTTTTAAACCGCCCTGGTCATCTGCCAACGCATAAGGCATGACAAGaaaa                 <  2:3163928/140‑1 (MQ=255)

                                                                                                                      ttGCGAAATCAACGGTCATT‑TTT‑TT‑TGCATCCTCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATCCTGTTTTAAACCGCCCTGGTCATCTGCCAACGCATAAGGCATGACAAGAAAACtt              <  2:295075/140‑1 (MQ=255)

                                                                                                                        gCGAAATCAACGGTCATT‑TTT‑TT‑TGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATCCTGTTTTAAACCGCCCTGGTCATCTGCCAACGCATAAGGCATGACAAGAAAACTTGc            <  1:3074346/140‑1 (MQ=255)

                                                                                                                        gCGAAAGCAACGGTCATT‑TTT‑TT‑TGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATCCTGTTTTAAACCGCCCTGGTCATCTGCCAACGCATAAGGCATGACAAGAAAACTTGc            <  1:2000401/140‑1 (MQ=255)

                                                                                                                        ccgcaaTCAACGGTCATT‑TTT‑TT‑TGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATCCTGTTTTAAACCGCCCTGGTCATCTGCCAACGCATAAGGCATGACAAGAAAACTTGc            <  2:4894973/136‑1 (MQ=255)

                                                                                                                                aaCGGTCATT‑TTT‑TT‑TGCATCATTGGTTCCGCGATACCCATCTTCTATTGCATGAGGCTGCGGTGGCGCTGCATCCTGTTTTAATCCGCACTGGTCATCTGACAACGCATAAGTCATGACAAGAAAACTTGCTCATATaa    <  2:4679477/140‑1 (MQ=255)

                                                                                                                                aaCGGTCATT‑TTT‑TT‑TGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATCCTGTTTTAAACCGCCCTGGTCATCTGCCAACGCATAAGGCATGACAAGAAAACTTGCTAATACaa    <  1:3418146/140‑1 (MQ=255)

                                                                                                                                aaCGGTCATT‑TTT‑TT‑TGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATCCTGTTTTAAACCGCCCTGGTCATCTGCCAACGCATAAGGAATGACAAGAAAACTTGCTAATACaa    <  2:1109621/140‑1 (MQ=255)

                                                                                                                                  cGGTCATT‑TTT‑TT‑TGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATCCTGTTTTAAACCGCCCTGGTCATCTGCCAACGCATAAGGCATGACAAGAAAACTTGCTAATACAATg  <  1:4636592/140‑1 (MQ=255)

GCCGCAGGAATAACGACATTAATTTCACCGCTCTTATCGCGAAAAACGTAACGGTCCTCTCCTTTGTGAGAAATCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCA‑T‑TTT‑TT‑TGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATCCTGTTTTAAACCGCCCTGGTCATCTGCCAACGCATAAGGCATGACAAGAAAACTTGCTAATACAATG  >  NZ_CP025268/1629034‑1629302

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

CGCAGGAATAACGACATTAATTTCACCGCTCTTATCGCGAAAAACGTAACGGTCCTCTCCTTTGTGAGAAATCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCA‑TTTTTTTGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATCCTGTTTTAAACCGCCCTGGTCATCTGCCAACGCATAAGGCATGACAAGAAAACTTGCTAATACAATGGCCTGAAATTTCATACTAACTC  >  NZ_CP025268/1629036‑1629324

CGCAGGAATAACGAGATTAATTTCACCGCTCTTATCGCGAAAAACGTAACGGTCCTCTCCTTTGTGAGAAATCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCATTTTTTTTGCATCATCG                                                                                                                                             >  SRR14920167.3568719/1‑151 (MQ=60)

    GGAATAACGACATTAATTTCACCGCTCTTATCGCGAAAAACGTAACGGTCCTCTCCTTTGTGAGAAATCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCATTTTTTTTGCATCATCGGTTC                                                                                                                                         <  SRR14920167.4770938/151‑1 (MQ=60)

    GGAATAACGACATTAATTTCACCGCTCTTATCGCGAAAAACGTAACGGTCCTCTCCTTTGTGAGAAATCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCATTTTTTTTGCATCATCGGTTC                                                                                                                                         >  SRR14920167.1951438/1‑151 (MQ=60)

     GAATAACGACATTAATTTCACCGCTCTTATCGCGAAAAACGTAACGGTCCTCTCCTTTGTGAGAAATCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCATTTTTTTTGCATCATCGGTTCC                                                                                                                                        >  SRR14920167.3170775/1‑151 (MQ=60)

             ACATTAATTTCACCGCTCTTATCGCGAAAAACGTAACGGTCCTCTCCTTTGTGAGAAATCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCATTTTTTTTGCATCATCGGTTCCGCGATAGC                                                                                                                                <  SRR14920167.2657590/151‑1 (MQ=60)

               ATTAATTTCACCGCTCTTATCGCGAAAAACGTAACGGTCCTCTCCTTTGTGAGAAATCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCATTTTTTTTGCATCATCGGTTCCGCGATAGCCA                                                                                                                              <  SRR14920167.4597010/151‑1 (MQ=60)

                 TAATTTCACCGCTCTTATCGCGAAAAACGTAACGGTCCTCTCCTTTGTGAGAAATCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCATTTTTTTTGCATCATCGGTTCCGCGATAGCCATC                                                                                                                            >  SRR14920167.2056948/1‑151 (MQ=60)

                 TAATTTCACCGCTCTTATCGCGAAAAACGTAACGGTCCTCTCCTTTGTGAGAAATCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCATTTTTTTTGCATCATCGGTTCCGCGATAGCCATC                                                                                                                            <  SRR14920167.4352100/151‑1 (MQ=60)

                     TTCACCGCTCTTATCGCGAAAAACGTAACGGTCCTCTCCTTTGTGAGAAATCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCATTTTTTTTGCATCATCGGTTCCGCGATAGCCATCTTCT                                                                                                                        <  SRR14920167.5191154/151‑1 (MQ=60)

                      TCACCGCTCTTATCGCGAAAAACGTAACGGTCCTCTCCTTTGTGAGAAATCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCATTTTTTTTGCATCATCGGTTCCGCGATAGCCATCTTCTA                                                                                                                       >  SRR14920167.1883664/1‑151 (MQ=60)

                       CACCGCTCTTATCGCGAAAAACGTAACGGTCCTCTCCTTTGTGAGAAATCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCATTTTTTTTGCATCATCGGTTCCGCGATAGCCATCTTCTAT                                                                                                                      <  SRR14920167.169922/151‑1 (MQ=60)

                         CCGCTCTTATCGCGAAAAACGTAACGGTCCTCTCCTTTGTGAGAAATCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCATTTTTTTTGCATCATCGGTTCCGCGATAGCCATCTTCTATTG                                                                                                                    <  SRR14920167.947279/151‑1 (MQ=60)

                         CCGCTCTTATCGCGAAAAACGTAACGGTCCTCTCCTTTGTGAGAAATCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCATTTTTTTTGCATCATCGGTTCCGCGATAGCCATCTTCTATTG                                                                                                                    <  SRR14920167.3298172/151‑1 (MQ=60)

                         CCGCTCTTATCGCGAAAAACGTAACGGTCCTCTCCTTTGTGAGAAATCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCATTTTTTTTGCATCATCGGTTCCGCGATAGCCATCTTCTATTG                                                                                                                    <  SRR14920167.1067332/151‑1 (MQ=60)

                         CCGCTCTTATCGCGAAAAACGTAACGGTCCTCTCCTTTGTGAGAAATCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCATTTTTTTTGCATCATCGGTTCCGCGATAGCCATCTTCTATTG                                                                                                                    <  SRR14920167.4966688/151‑1 (MQ=60)

                           GCTCTTATCGCGAAAAACGTAACGGTCCTCTCCTTTGTGAGAAATCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCATTTTTTTTGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCA                                                                                                                  <  SRR14920167.2286596/151‑1 (MQ=60)

                            CTCTTATCGCGCAAAACGTAACGGTCCGCGCCTTTGTGAGAAATCAAATTACCGCGTAGTGAAACCGAAGCCCCATCGGGCCTGGTTGTTGCGAAATCAACGGTCACTTTTTTTGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCAT                                                                                                                 <  SRR14920167.4512415/151‑1 (MQ=60)

                            CTCTTATCGCGAAAAACGTAACGGTCCTCTCCTTTGTGAGAAATCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCATTTTTTTTGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCAT                                                                                                                 >  SRR14920167.3139618/1‑151 (MQ=60)

                            CTCTTATCGCGAAAAACGTAACGGTCCTCTCCTTTGTGAGAAATCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCATTTTTTTTGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCAT                                                                                                                 <  SRR14920167.2345019/151‑1 (MQ=60)

                              CTTATCGCGAAAAACGTAACGGTCCTCTCCTTTGTGAGAAATCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCATTTTTTTTGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGA                                                                                                               <  SRR14920167.3820996/151‑1 (MQ=60)

                              CTTATCGCGAAAAACGTAACGGTCCTCTCCTTTGTGAGAAATCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCATTTTTTTTGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGA                                                                                                               >  SRR14920167.1126392/1‑151 (MQ=60)

                                TATCGCGAAAACCGTAACGGTCCACTCCTTTGTGAGAAATCAAATTACCGCGTAGTGAATCCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCATTTTTTTTGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGG                                                                                                             <  SRR14920167.5080858/151‑1 (MQ=60)

                                      GAAAAACGTAACGGTCCTCTCCTTTGTGAGAAATCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCATTTTTTTTGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGG                                                                                                       <  SRR14920167.2643705/151‑1 (MQ=60)

                                      GAAAAACGTAACGGTCCTCTCCTTTGTGAGAAATCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCATTTTTTTTGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGG                                                                                                       <  SRR14920167.3850616/151‑1 (MQ=60)

                                       AAAAACGTAACGGTCCTCTCCTTTGTGAGAAATCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCATTTTTTTTGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGT                                                                                                      <  SRR14920167.4803396/151‑1 (MQ=60)

                                         AAACGTAACGGTCCTCTCCTTTGTGAGAAATCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCATTTTTTTTGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGG                                                                                                    >  SRR14920167.3062939/1‑151 (MQ=60)

                                              TAACGGTCCTCTCCTTTGTGAGAAATCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCATTTTTTTTGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTG                                                                                               <  SRR14920167.2273254/151‑1 (MQ=60)

                                              TAACGGTCCTCTCCTTTGTGAGAAATCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCATTTTTTTTGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTG                                                                                               <  SRR14920167.3930585/151‑1 (MQ=60)

                                                 CGGTCCTCTCCTTTGTGAGAAATCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCATTTTTTTTGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCAT                                                                                            <  SRR14920167.4138956/151‑1 (MQ=60)

                                                   GTCCTCTCCTTTGTGAGAAATCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCATTTTTTTTGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATCC                                                                                          <  SRR14920167.5127406/151‑1 (MQ=60)

                                                   GTCCTCTCCTTTGTGAGAAATCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCATTTTTTTTGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATCC                                                                                          >  SRR14920167.5587132/1‑151 (MQ=60)

                                                          CCTTTGTGAGAAATCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCACTTTTTTTGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATCCTGTTTTA                                                                                   >  SRR14920167.4451512/1‑151 (MQ=60)

                                                             TTGTGAGAAATCAAATTTCCGCGTCGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCATTTTTTTTGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATCCTGTTTTAAAC                                                                                <  SRR14920167.3284717/151‑1 (MQ=60)

                                                             TTGTGAGAAATCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCATTTTTTTTGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATCCTGTTTTAAAC                                                                                <  SRR14920167.864121/151‑1 (MQ=60)

                                                             TTGTGAGAAATCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCATTTTTTTTGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATCCTGTTTTAAAC                                                                                <  SRR14920167.4299589/151‑1 (MQ=60)

                                                             TTGTGAGAAATCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCATTTTTTTTGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATCCTGTTTTAAAC                                                                                <  SRR14920167.4060738/151‑1 (MQ=60)

                                                             TTGTGAGAAATCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCATTTTTTTTGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATCCTGTTTTAAAC                                                                                <  SRR14920167.1461066/151‑1 (MQ=60)

                                                             TTGTGAGAAATCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCATTTTTTTTGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATCCTGTTTTAAAC                                                                                <  SRR14920167.1445986/151‑1 (MQ=60)

                                                             TTGTGAGAAATCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCATTTTTTTTGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATCCTGTTTTAAAC                                                                                <  SRR14920167.817441/151‑1 (MQ=60)

                                                              TGTGAGAAATCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCATTTTTTTTGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATCCTGTTTTAAACC                                                                               <  SRR14920167.3729240/151‑1 (MQ=60)

                                                                   GAAATCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCATTTTTTTTGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATCCTGTTTTAAACCGCCCT                                                                          >  SRR14920167.4715793/1‑151 (MQ=60)

                                                                       TCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCATTTTTTTTGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATCCTGTTTTAAACCGCCCTGGTC                                                                      >  SRR14920167.4123491/1‑151 (MQ=60)

                                                                       TCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCATTTTTTTTGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATCCTGTTTTAAACCGCCCTGGTC                                                                      <  SRR14920167.4619867/151‑1 (MQ=60)

                                                                       TCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCATTTTTTTTGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATCCTGTTTTAAACCGCCCTGGTC                                                                      <  SRR14920167.427016/151‑1 (MQ=60)

                                                                            TTACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCATTTTTTTTGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATCCTGTTTTAAACCGCCCTGGTCATCTG                                                                 <  SRR14920167.4905723/151‑1 (MQ=60)

                                                                            TTACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCATTTTTTTTGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATCCTGTTTTAAACCGCCCTGGTCATCTG                                                                 <  SRR14920167.2986645/151‑1 (MQ=60)

                                                                            TTACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCATTTTTTTTGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATCCTGTTTTAAACCGCCCTGGTCATCTG                                                                 <  SRR14920167.3425666/151‑1 (MQ=60)

                                                                            TTACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCATTTTTTTTGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATCCTGTTTTAAACCGCCCTGGTCATCTG                                                                 <  SRR14920167.1603117/151‑1 (MQ=60)

                                                                                         AAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCATTTTTTTTGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATCCTGTTTTAAACCGCCCTGGTCATCTGCCAACGCATAAGG                                                    <  SRR14920167.4054246/151‑1 (MQ=60)

                                                                                         AAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCATTTTTTTTGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATCCTGTTTTAAACCGCCCTGGTCATCTGCCAACGCATAAGG                                                    <  SRR14920167.1855865/151‑1 (MQ=60)

                                                                                          AACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCATTTTTTTTGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATCCTGTTTTAAACCGCCCTGGTCATCTGCCAACGCATAAGGC                                                   <  SRR14920167.5230998/151‑1 (MQ=60)

                                                                                          AACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCATTTTTTTTGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATCCTGTTTTAAACCGCCCTGGTCATCTGCCAACGCATAAGGC                                                   <  SRR14920167.4023468/151‑1 (MQ=60)

                                                                                          AACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCATTTTTTTTGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATCCTGTTTTAAACCGCCCTGGTCATCTGCCAACGCATAAGGC                                                   <  SRR14920167.3968528/151‑1 (MQ=60)

                                                                                               AAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCATTTTTTTTGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATCCTGTTTTAAACCGCCCTGGTCATCTGCCAACGCATAAGGCATGAC                                              <  SRR14920167.5273923/151‑1 (MQ=60)

                                                                                               AAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCATTTTTTTTGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATCCTGTTTTAAACCGCCCTGGTCATCTGCCAACGCATAAGGCATGAC                                              <  SRR14920167.2616569/151‑1 (MQ=60)

                                                                                               AAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCATTTTTTTTGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATCCTGTTTTAAACCGCCCTGGTCATCTGCCAACGCATAAGGCATGAC                                              >  SRR14920167.2426676/1‑151 (MQ=60)

                                                                                                 GCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCATTTTTTTTGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATCCTGTTTTAAACCGCCCTGGTCATCTGCCAACGCATAAGGCATGACAA                                            >  SRR14920167.1079292/1‑151 (MQ=60)

                                                                                                       TCGTGCATGGTTTTTGCGAAATCAACGGTCATTTTTTTTGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATCCTGTTTTAAACCGCCCTGGTCATCTGCCAACGCATAAGGCATGACAAGAAAAC                                      <  SRR14920167.3685566/151‑1 (MQ=60)

                                                                                                       TCGTGCATGGTTTTTGCGAAATCAACGGTCATTTTTTTTGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATCCTGTTTTAAACCGCCCTGGTCATCTGCCAACGCATAAGGCATGACAAGAAAAC                                      <  SRR14920167.127433/151‑1 (MQ=60)

                                                                                                       TCGTGCATGGTTTTTGCGAAATCAACGGTCATTTTTTTTGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATCCTGTTTTAAACCGCCCTGGTCATCTGCCAACGCATAAGGCATGACAAGAAAAC                                      <  SRR14920167.3830306/151‑1 (MQ=60)

                                                                                                        CGTGCATGGTTTTTGCGAAATCAACGGTCATTTTTTTTGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATCCTGTTTTAAACCGCCCTGGTCATCTGCCAACGCATAAGGCATGACAAGAAAACT                                     <  SRR14920167.1423025/151‑1 (MQ=60)

                                                                                                              TGGTTTTTGCGAAATCAACGGTCATTTTTTTTGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATCCTGTTTTAAACCGCCCTGGTCATCTGCCAACGCATAAGGCATGACAAGAAAACTTGCTAA                               <  SRR14920167.1554722/151‑1 (MQ=60)

                                                                                                                 TTTTTGCGAAATCAACGGTCATTTTTTTTGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATCCTGTTTTAAACCGCCCTGGTCATCTGCCAACGCATAAGGCATGACAAGAAAACTTGCTAATAC                            <  SRR14920167.1559755/151‑1 (MQ=60)

                                                                                                                 TTTTTGCGAAATCAACGGTCATTTTTTTTGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATCCTGTTTTAAACCGCCCTGGTCATCTGCCAACGCATAAGGCATGACAAGAAAACTTGCTAATAC                            <  SRR14920167.4524069/151‑1 (MQ=60)

                                                                                                                 TTTTTGCGAAATCAACGGTCATTTTTTTTGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATCCTGTTTTAAACCGCCCTGGTCATCTGCCAACGCATAAGGCATGACAAGAAAACTTGCTAATAC                            <  SRR14920167.2236257/151‑1 (MQ=60)

                                                                                                                 TTTTTGCGAAATCAACGGTCATTTTTTTTGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATCCTGTTTTAAACCGCCCTGGTCATCTGCCAACGCATAAGGCATGACAAGAAAACTTGCTAATAC                            <  SRR14920167.2743312/151‑1 (MQ=60)

                                                                                                                      GCGAAATCAACGGTCATTTTTTTTGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATCCTGTTTTAAACCGCCCTGGTCATCTGCCAACGCATAAGGCATGACAAGAAAACTTGCTAATACAATGG                       <  SRR14920167.3080708/151‑1 (MQ=60)

                                                                                                                      GCGAAAGCAACGGTCATTTTTTTTGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATCCTGTTTTAAACCGCCCTGGTCATCTGCCAACGCATAAGGCATGACAAGAAAACTTGCTAATACAATGG                       <  SRR14920167.2004556/151‑1 (MQ=60)

                                                                                                                              AACGGTCATTTTTTTTGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATCCTGTTTTAAACCGCCCTGGTCATCTGCCAACGCATAAGGCATGACAAGAAAACTTGCTAATACAATGGCCTGAAAT               <  SRR14920167.3425716/151‑1 (MQ=60)

                                                                                                                                CGGTCATTTTTTTTGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATCCTGTTTTAAACCGCCCTGGTCATCTGCCAACGCATAAGGCATGACAAGAAAACTTGCTAATACAATGGCCTGAAATTT             <  SRR14920167.4647501/151‑1 (MQ=60)

                                                                                                                                CGGTCATTTTTTTTGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATCCTGTTTTAAACCGCCCTGGTCATCTGCCAACGCATAAGGCATGACAAGAAAACTTGCTAATACAATGGCCTGAAATTT             <  SRR14920167.4647005/151‑1 (MQ=60)

                                                                                                                                     atTTTTTTTGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATCCTGTTTTAAACCGCCCTGGTCATCTGCCAACGCATAAGGCATGACAAGAAAACTTGCTAATACAATGGCCTGAAATTTCATAC        <  SRR14920167.2732491/149‑1 (MQ=60)

                                                                                                                                           TTTGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATCCTGTTTTAAACCGCCCTGGTCATCTGCCAACGCATAAGGCATGACAAGAAAACTTGCTAATACAATGGCCTGAAATTTCATACTAACTC  <  SRR14920167.5421127/151‑1 (MQ=60)

                                                                                                                                           TTTGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATCCTGTTTTAAACCGCCCTGGTCATCTGCCAACGCATAAGGCATGACAAGAAAACTTGCTAATACAATGGCCTGAAATTTCATACTAACTC  <  SRR14920167.5421360/151‑1 (MQ=60)

                                                                                                                                           TTTGCATCATCGCTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATCCTGTTTTAAACCGCCCTGGTCATCTGCCAACGCATAAGGCATGACAAGAAAACTTGCTAATACAATGGCCTGAAATTTCATACTAACTC  <  SRR14920167.4797951/151‑1 (MQ=60)

                                                                                                                                           TTTGCATCATCGCTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATCCTGTTTTAAACCGCCCTGGTCATCTGCCAACGCATAAGGCATGACAAGAAAACTTGCTAATACAATGGCCTGAAATTTCATACTAACTC  <  SRR14920167.4798021/151‑1 (MQ=60)

CGCAGGAATAACGACATTAATTTCACCGCTCTTATCGCGAAAAACGTAACGGTCCTCTCCTTTGTGAGAAATCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCA‑TTTTTTTGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATCCTGTTTTAAACCGCCCTGGTCATCTGCCAACGCATAAGGCATGACAAGAAAACTTGCTAATACAATGGCCTGAAATTTCATACTAACTC  >  NZ_CP025268/1629036‑1629324

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`