Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F50 I1 R1	775	316.5	10245680	97.2%	9958800	138.8

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	4,542,898	(A)_6→7	pseudogene (27/602 nt)	fimB →	type 1 fimbria switch DNA invertase FimB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	4,542,892	1	.	A	97.6%	471.6 / 3.0	125	pseudogene (21/602 nt)	fimB	type 1 fimbria switch DNA invertase FimB
Reads supporting (aligned to +/- strand): ref base . (3/0); new base A (77/45); total (80/45)
Fisher's exact test for biased strand distribution p-value = 5.52e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.69e-01

Only 100 of 134 total aligned reads displayed.

CCACTTACGTTAGTTATTAAGCAAACGTTCGCTTTTATAAACATAATCAGGATAAAAATGTTGGATTATTGCTAACCCAGCACAGCTAGTGCGCGTCTGTAATTATAAGGGAAAAACGATGAAGAATAAGGCTGATAAC‑AAA‑AAAGGAACTTCCTGACCCATAGTGAAATCGAATCACTCCTTAAAGCAGCAAATACCGGGCCTCATGCAGCACGTAATTATTGTCTGACTTTGCTTTGTTTTATTCATGGTTTCCGGGCGAGTGAAATTTGT  >  NZ_CP025268/4542754‑4543026

ccACTTACGTTAGTTATTAAGCAAACGTTCGCTTTTATAAACATAATCAGTATAAAAATGTTGGATTATTGCTAACCCAGCACAGCTAGTGCGCGTCTGTAATTATAAGGGAAAAACGAGGAAGAATAAGGCGGATAAC‑a                                                                                                                                        >  2:2206360/1‑140 (MQ=255)

ccACTTACGTTAGTTATTAAGCAAACGTTCGCTTTTATAAACATAATCAGGATAAAAATGTTGGATTATTGCTAACCCAGCACAGCTAGTGCGCGTCTGTAATTATAAGGGAAAAACGATGAAGAATAAGGCTGATAAC‑a                                                                                                                                        >  2:3541721/1‑140 (MQ=255)

ccACTTACGTTAGTTATTAAGCAAACGTTCGCTTTTATAAACATAATCAGGATAAAAATGTTGGATTATTGCTAACCCAGCACAGCTAGTGCGCGTCTGTAATTATAAGGGAAAAACGATGAAGAATAAGGCTGATAAC‑a                                                                                                                                        >  1:2720578/1‑140 (MQ=255)

   cTTACGTTAGTTATTAAGCAAACGTTCGCTTTTATAAACATAATCAGGATAAAAATGTTGGATTATTGCTAACCCAGCACAGCTAGTGCGCGTCTGTAATTATAAGGGAAAAACGATGAAGAATAAGGCTGATAAC‑aaa‑a                                                                                                                                    >  2:4197819/1‑140 (MQ=255)

   cTTACGTTAGTTATTAAGCAAACGTTCGCTTTTATAAACATAATCAGGATAAAAATGTTGGATTATTGCTAACCCAGCACAGCTAGTGCGCGTCTGTAATTATAAGGGAAAAACGATGAAGAATAAGGCTGATAAC‑aaa‑a                                                                                                                                    >  1:1438017/1‑140 (MQ=255)

      aCGTTAGTTATTAAGCAAACGTTCGCTTTTATAAACATAATCAGGATAAAAATGTTGGATTATTGCTAACCCAGCACAGCTAGTGCGCGTCTGTAATTATAAGGGAAAAACGATGAAGAATAAGGCTGATAAC‑aaa‑aaaa                                                                                                                                 >  2:211557/1‑139 (MQ=255)

         ttagttaTTAAGCAAACGTTCGCTTTTATAAACATAATCAGGATAAAAATGTTGGATTATTGCTAACCCAGCACAGCTAGTGCGCGTCTGTAATTATAAGGGAAAAACGATGAAGAATAAGGCTGATAAC‑aaa‑aaaagga                                                                                                                              <  1:694002/140‑5 (MQ=255)

             ttattaAGCAAACGTTCGCTTTTATAAACATAATCAGGATAAAAATGTTGGATTATTGCTAACCCAGCACAGCTAGTGCGCGTCTGTAATTATAAGGGAAAAACGATGAAGAATAAGGCTGATAACAAAA‑AAAGGAACtt                                                                                                                           >  2:3507462/1‑140 (MQ=255)

             ttattaAGCAAACGTTCGCTTTTATAAACATAATCAGGATAAAAATGTTGGATTATTGCTAACCCAGCACAGCTAGTGCGCGTCTGTAATTATAAGGGAAAAACGATGAAGAATAAGGCTGATAACAAAA‑AAAGGAACtt                                                                                                                           >  1:2257166/1‑140 (MQ=255)

              tattaAGCAAACGTTCGCTTTTATAAACATAATCAGGATAAAAATGTTGGATTATTGCTAACCCAGCACAGCTAGTGCGCGTCTGTAATTATAAGGGAAAAACGATGAAGAATAAGGCTGATAACAAAA‑AAAGGAACTTc                                                                                                                          <  2:12429/140‑1 (MQ=255)

               attaAGCAAACGTTCGCTTTTATAAACATAATCAGGATAAAAATGTTGGATTATTGCTAACCCAGCACAGCTAGTGCGCGTCTGTAATTATAAGGGAAAAACGATGAAGAATAAGGCTGATAACAAAA‑AAAGGAACTTcc                                                                                                                         <  2:407611/140‑1 (MQ=255)

                ttaAGCAAACGTTCGCTTTTATAAACATAATCAGGATAAAAATGTTGGATTATTGCTAACCCAGCACAGCTAGTGCGCGTCTGTAATTATAAGGGAAAAACGATGAAGAATAAGGCTGATAACAAAA‑AAAGGAACTTCCt                                                                                                                        <  1:5044965/140‑1 (MQ=255)

                  aaGCAAACGTTCGCTTTTATAAACATAATCAGGATAAAAATGTTGGATTATTGCTAACCCAGCACAGCTAGTGCGCGTCTGTAATTATAAGGGAAAAACGATGAAGAATAAGGCTGATAACAAAA‑AAAGGAACTTCCTGa                                                                                                                      >  2:1701938/1‑140 (MQ=255)

                  aaGCAAACGTTCGCTTTTATAAACATAATCAGGATAAAAACGTTGGATTATTGCTAACCCAGCACAGCTAGTGCGCGTCTGTAATTATAAGGGAAAAACGATGAAGAATAAGGCTGATAACAAAA‑AAAGGAACTTCCTGa                                                                                                                      >  2:1288909/1‑140 (MQ=255)

                       aaCGTTCGCTTTTATAAACATAATCAGGATAAAAATGTTGGATTATTGCTAACCCAGCACAGCTAGTGCGCGTCTGTAATTATAAGGGAAAAACGATGAAGAATAAGGCTGATAACAAAA‑AAAGGAACTTCCTGACCCAt                                                                                                                 >  1:2507579/1‑140 (MQ=255)

                       aaCGTTCGCTTTTATAAACATAATCAGGATAAAAATGTTGGATTATTGCTAACCCAGCACAGCTAGTGCGCGTCTGTAATTATAAGGGAAAAACGATGAAGAATAAGGCTGATAACAAAA‑AAAGGAACTTCCTGACCCAt                                                                                                                 >  2:58700/1‑140 (MQ=255)

                        aCGTTCGCTTTTATAAACATAATCAGGATAAAAATGTTGGATTATTGCTAACCCAGCACAGCTAGTGCGCGTCTGTAATTATAAGGGAAAAACGATGAAGAATAAGGCTGATAACAAAA‑AAAGGAACTTCCTGACCCATa                                                                                                                >  2:1033129/1‑140 (MQ=255)

                             cGCTTTTATAAACATAATCAGGATAAAAATGTTGGATTATTGCTAACCCAGCACAGCTAGTGCGCGTCTGTAATTATAAGGGAAAAACGATGAAGAATAAGGCTGATAACAAAA‑AAAGGAACTTCCTGACCCATAGTGaa                                                                                                           >  1:1167333/1‑140 (MQ=255)

                               cTTTTATAAACATAATCAGGATAAAAATGTTGGATTATTGCTAACCCAGCACAGCTAGTGCGCGTCTGTAATTATAAGGGAAAAACGATGAAGAATAAGGCTGATAACAAAA‑AAAGGAACTTCCTGACCCATAGTGAAAt                                                                                                         <  2:2720578/140‑1 (MQ=255)

                                    ataAACATAATCAGGATAAAAATGTTGGATTATTGCTAACCCAGCACAGCTAGTGCGCGTCTGTAATTATAAGGGAAAAACGATGAAGAATAAGGCTGATAACAAAA‑AAAGGAACTTCCTGACCCATAGTGAAATCGAAt                                                                                                    <  2:2946482/140‑1 (MQ=255)

                                    ataAACATAATCAGGATAAAAATGTTGGATTATTGCTAACCCAGCACAGCTAGTGCGCGTCTGTAATTATAAGGGAAAAACGATGAAGAATAAGGCTGATAACAAAA‑AAAGGAACTTCCTGACCCATAGTGAAATCGAAt                                                                                                    <  1:533041/140‑1 (MQ=255)

                                    ataAACATAATCAGGATAAAAATGTTGGATTATTGCTAACCCAGCACAGCTAGTGCGCGTCTGTAATTATAAGGGAAAAACGATGAAGAATAAGGCTGATAACAAAA‑AAAGGAACTTCCTGACCCATAGTGAAATCGAAt                                                                                                    <  1:3152102/140‑1 (MQ=255)

                                      aaaCATAATCAGGATAAAAATGTTGGATTATTGCTAACCCAGCACAGCTAGTGCGCGTCTGTAATTATAAGGGAAAAACGATGAAGAATAAGGCTGATAACAAAA‑AAAGGAACTTCCTGACCCATAGTGAAATCGAATCa                                                                                                  <  2:3658858/140‑1 (MQ=255)

                                      aaaCATAATCAGGATAAAAATGTTGGATTATTGCTAACCCAGCACAGCTAGTGCGCGTCTGTAATTATAAGGGAAAAACGATGAAGAATAAGGCTGATAACAAAA‑AAAGGAACTTCCTGACCCATAGTGAAATCGAATCa                                                                                                  <  1:1212025/140‑1 (MQ=255)

                                      aaaCATAATCAGGATAAAAATGTTGGATTATTGCTAACCCAGCACAGCTAGTGCGCGTCTGTAATTATAAGGGAAAAACGATGAAGAATAAGGCTGATAACAAAA‑AAAGGAACTTCCTGACCCATAGTGAAATCGAATCa                                                                                                  <  1:1212084/140‑1 (MQ=255)

                                          aTAATCAGGATAAAAATGTTGGATTATTGCTAACCCAGCACAGCTAGTGCGCGTCTGTAATTATAAGGGAAAAACGATGAAGAATAAGGCTGATAACAAAA‑AAAGGAACTTCCTGACCCATAGTGAAATCGAATCa                                                                                                  <  2:5011140/136‑1 (MQ=255)

                                          aTAATCAGGATAAAAATGTTGGATTATTGCTAACCCAGCACAGCTAGTGCGCGTCTGTAATTATAAGGGAAAAACGATGAAGAATAAGGCTGATAACAAAA‑AAAGGAACTTCCTGACCCATAGTGAAATCGAATCa                                                                                                  >  1:5011140/1‑136 (MQ=255)

                                            aaTCAGGATAAAAATGTTGGATTATTGCTAACCCAGCACAGCTAGTGCGCGTCTGTAATTATAAGGGAAAAACGATGAAGAATAAGGCTGATAACAAAA‑AAAGGAACTTCCTGACCCATAGTGAAATCGAATCACTCCtt                                                                                            >  2:3788052/1‑140 (MQ=255)

                                               cAGGATAAAAATGTTGGATTATTGCTAACCCAGCACAGCTAGTGCGCGTCTGTAATTATAAGGGAAAAACGATGAAGAATAAGGCTGATAACAAAA‑AAAGGAACTTCCTGACCCATAGTGAAATCGAATCACTCCTTaaa                                                                                         <  2:3125255/140‑1 (MQ=255)

                                               cAGGATAAAAATGTTGGATTATTGCTAACCCAGCACAGCTAGTGCGCGTCTGTAATTATAAGGGAAAAACGATGAAGAATAAGGCTGATAACAAAA‑AAAGGAACTTCCTGACCCATAGTGAAATCGAATCACTCCTTaaa                                                                                         <  1:3138454/140‑1 (MQ=255)

                                                           gTTGGATTATTGCTAACCCAGCACAGCTAGTGCGCGTCTGTAATTATAAGGGAAAAACGATGAAGAATAAGGCTGATAACAAAA‑AAAGGAACTTCCTGACCCATAGTGAAATCGAATCACTCCTTAAAGCAGCAAATAcc                                                                             <  2:1545897/140‑1 (MQ=255)

                                                           gTTGGATTATTGCTAACCCAGCACAGCTAGTGCGCGTCTGTAATTATAAGGGAAAAACGATGAAGAATAAGGCTGATAACAAAA‑AAAGGAACTTCCTGACCCATAGTGAAATCGAATCACTCCTTAAAGCAGCAAATAcc                                                                             <  2:570645/140‑1 (MQ=255)

                                                           gTTGGATTATTGCTAACCCAGCACAGCTAGTGCGCGTCTGTAATTATAAGGGAAAAACGATGAAGAATAAGGCTGATAACAAAA‑AAAGGAACTTCCTGACCCATAGTGAAATCGAATCACTCCTTAAAGCAGCAAATAcc                                                                             <  2:4571577/140‑1 (MQ=255)

                                                            ttGGATTATTGCTAACCCAGCACAGCTAGTGCGCGTCTGTAATTATAAGGGAAAAACGATGAAGAATAAGGCTGATAACAAAA‑AAAGGAACTTCCTGACCCATAGTGAAATCGAATCACTCCTTAAAGCAGCAAATACCg                                                                            >  1:3833564/1‑140 (MQ=255)

                                                            ttGGATTATTGCTAACCCAGCACAGCTAGTGCGCGTCTGTAATTATAAGGGAAAAACGATGAAGAATAAGGCTGATAACAAAA‑AAAGGAACTTCCTGACCCATAGTGAAATCGAATCACTCCTTAAAGCAGCAAATACCg                                                                            >  1:1970019/1‑140 (MQ=255)

                                                             tGGATTATTGCTAACCCAGCACAGCTAGTGCGCGTCTGTAATTATAAGGGAAAAACGATGAAGAATAAGGCTGATAACAAAA‑AAAGGAACTTCCTGACCCATAGTGAAATCGAATCACTCCTTAAAGCAGCAAATACCgg                                                                           <  1:2634388/140‑1 (MQ=255)

                                                             tGGATTATTGCTAACCCAGCACAGCTAGTGCGCGTCTGTAATTATAAGGGAAAAACGATGAAGAATAAGGCTGATAACAAAA‑AAAGGAACTTCCTGACCCATAGTGAAATCGAATCACTCCTTAAAGCAGCAAATACCgg                                                                           <  1:58700/140‑1 (MQ=255)

                                                                  tattGCTAACCCAGCACAGCTAGTGCGCGTCTGTAATTATAAGGGAAAAACGATGAAGAATAAGGCTGATAACAAAA‑AAAGGAACTTCCTGCCCCATAGTGAAATCGACTCACTCCTTAAAGCAGCAAATACCGGGCCTc                                                                      <  2:1289900/140‑1 (MQ=255)

                                                                    ttGCTAACCCAGCACAGCTAGTGCGCGTCTGTAATTATAAGGGAAAAACGATGAAGAATAAGGCTGATAACAAAA‑AAAGGAACTTCCTGACCCATAGTGAAATCGAATCACTCCTTAAAGCAGCAAATACCGGGCCTCAt                                                                    >  2:4335022/1‑140 (MQ=255)

                                                                      gCTAACCCAGCACAGCTAGTGCGCGTCTGTAATTATAAGGGAAAAACGATGAAGAATAAGGCTGATAACAAAA‑AAAGGAACTTCCTGACCCATAGTGAAATCGAATCACTCCTTAAAGCAGCAAATACCGGGCCTCATgc                                                                  <  1:2028751/140‑1 (MQ=255)

                                                                        tAACCCAGCACAGCTAGTGCGCGTCTGTAATTATAAGGGAAAAACGATGAAGAATAAGGCTGATAACAAAA‑AAAGGAACTTCCTGACCCATAGTGAAATCGAATCACTCCTTAAAGCAGCAAATACCGGGCCTCATgcag                                                                <  2:965688/140‑1 (MQ=255)

                                                                        tAACCCAGCACAGCTAGTGCGCGTCTGTAATTATAAGGGAAAAACGATGAAGAATAAGGCTGATAACAAAA‑AAAGGAACTTCCTGACCCATAGTGAAATCGAATCACTCCTTAAAGCAGCAAATACCGGGCCTCATgcag                                                                <  1:3715563/140‑1 (MQ=255)

                                                                        tAACCCAGCACAGCTAGTGCGCGTCTGTAATTATAAGGGAAAAACGATGAAGAATAAGGCTGATAACAAAA‑AAAGGAACTTCCTGACCCATAGTGAAATCGAATCACTCCTTAAAGCAGCAAATACCGGGCCTCATgcag                                                                <  2:4782987/140‑1 (MQ=255)

                                                                         aaCCCAGCACAGCTAGTGCGCGTCTGTAATTATAAGGGAAAAACGATGAAGAATAAGGCTGATAACAAAA‑AAAGGAACTTCCTGACCCATAGTGAAATCGAATCACTCCTTAAAGCAGCAAATACCGGGCCTCATgcagc                                                               >  1:209016/1‑140 (MQ=255)

                                                                          aCCCAGCACAGCTAGTGCGCGTCTGTAATTATAAGGGAAAAACGATGAAGAATAAGGCTGATAACAAAA‑AAAGGAACTTCCTGACCCATAGTGAAATCGAATCACTCCTTAAAGCAGCAAATACCGGGCCTCATgcagca                                                              <  1:2060031/140‑1 (MQ=255)

                                                                          aCCCAGCACAGCTAGTGCGCGTCTGTAATTATAAGGGAAAAACGATGAAGAATAAGGCTGATAACAAAA‑AAAGGAACTTCCTGACCCATAGTGAAATCGAATCACTCCTTAAAGCAGCAAATACCGGGCCTCATgcagca                                                              <  1:972089/140‑1 (MQ=255)

                                                                              aGCACAGCTAGTGCGCGTCTGTAATTATAAGGGAAAAACGATGAAGAATAAGGCTGATAACAAAA‑AAAGGAACTTCCTGACCCATAGTGAAATCGAATCACTCCTTAAAGCAGCAAATACCGGGCCTCATGCAGCACGTa                                                          >  2:2595400/1‑140 (MQ=255)

                                                                              aGCACAGCTAGTGCGCGTCTGTAATTATAAGGGAAAAACGATGAAGAATAAGGCTGATAACAAAA‑AAAGGAACTTCCTGACCCATAGTGAAATCGAATCACTCCTTAAAGCAGCAAATACCGGGCCTCATGCAGCACGTa                                                          >  1:475897/1‑140 (MQ=255)

                                                                              aGCACAGCTAGTGCGCGTCTGTAATTATAAGGGAAAAACGATGAAGAATAAGGCTGATAACAAAA‑AAAGGAACTTCCTGACCCATAGTGAAATCGAATCACTCCTTAAAGCAGCAAATACCGGGCCTCATGCAGCACGTa                                                          >  1:4107247/1‑140 (MQ=255)

                                                                               gCACAGCTAGTGCGCGTCTGTAATTATAAGGGAAAAACGATGAAGAATAAGGCTGATAACAAAA‑AAAGGAACTTCCTGACCCATAGTGAAATCGAATCACTCCTTAAAGCAGCAAATACCGGGCCTCATGCAGCACGTaa                                                         >  2:4265825/1‑140 (MQ=255)

                                                                                cacaGCTAGTGCGCGTCTGTAATTATAAGGGAAAAACGATGAAGAATAAGGCTGATAACAAAA‑AAAGGAACTTCCTGACCCATAGTGAAATCGAATCACTCCTTAAAGCAGCAAATACCGGGCCTCATGCAGCACGTAat                                                        >  2:706279/1‑140 (MQ=255)

                                                                                caaaGCTAGTGCGCGTCTGTAATTATAAGGG‑AAAACGATGAAGAATAAGGCTGATAACAAAA‑AAAGGAACTTCCTGACCCATAGTGAAATCGAATCACTCCTTAAAGCAGCAAAGACCGGGCCTCATGCAGCACGTaagt                                                       >  2:4613648/4‑138 (MQ=255)

                                                                                 acaGCTAGTGCGCGTCTGTAATTATAAGGGAAAAACGATGAAGAATAAGGCTGATAACAAAA‑AAAGGAACTTCCTGACCCATAGTGAAATCGAATCACTCCTTAAAGCAGCAAATACCGGGCCTCATGCAGCACGTAatt                                                       >  1:692847/1‑140 (MQ=255)

                                                                                     cTAGTGCGCGTCTGTAATTATAAGGGAAAAACGATGAAGAATAAGGCTGATAACAAAA‑AAAGGAACTTCCTGACCCATAGTGAAATCGAATCACTCCTTAAAgcagca                                                                                   <  2:1449832/108‑1 (MQ=255)

                                                                                     cTAGTGCGCGTCTGTAATTATAAGGGAAAAACGATGAAGAATAAGGCTGATAACAAAA‑AAAGGAACTTCCTGACCCATAGTGAAATCGAATCACTCCTTAAAgcagca                                                                                   >  1:1449832/1‑108 (MQ=255)

                                                                                     cTAGTGCGCGTCTGTAATTATAAGGGAAAAACGATGAAGAATAAGGCTGATAACAAAA‑AAAGGAACTTCCTGACCCATAGTGAAATCGAATCACTCCTTAAAGCAGCAAATACCGGGCCTCATGCAGCACGTAATTATTg                                                   >  1:2626113/1‑140 (MQ=255)

                                                                                     cTAGTGCGCGTCTGTAATTATAAGGGAAAAACGATGAAGAATAAGGCTGATAACAAAA‑AAAGGAACTTCCTGACCCATAGTGAAATCGAATCACTCCTTAAAGCAGCAAATACCGGGCCTCATGCAGCACGTAATTATTg                                                   >  2:1633036/1‑140 (MQ=255)

                                                                                     cTAGTGCGCGTCTGTAATTATAAGGGAAAAACGATGAAGAATAAGGCTGATAACAAAA‑AAAGGAACTTCCTGACCCATAGTGAAATCGAATCACTCCTTAAAGCAGCAAATACCGGGCCTCATGCAGCACGTAATTATTg                                                   >  2:3458846/1‑140 (MQ=255)

                                                                                     cTAGTGCGCGTCTGTAATTATAAGGGAAAAACGATGAAGAATAAGGCTGATAACAAAA‑AAAGGAACTTCCTGACCCATAGTGAAATCGAATCACTCCTTAAAGCAGCAAATACCGGGCCTCATGCAGCACGTAATTATTg                                                   >  1:598803/1‑140 (MQ=255)

                                                                                     cTAGTGCGCGTCTGTAATTATAAGGGAAAAACGATGAAGAATAAGGCTGATAACAAAA‑AAAGGAACTTCCTGACCCATAGTGAAATCGAATCACTCCTTAAAGCAGCAAATACCGGGCCTCATGCAGCACGTAATTATTg                                                   >  1:661090/1‑140 (MQ=255)

                                                                                     cTAGTGCGCGTCTGTAATTATAAGGGAAAAACGATGAAGAATAAGGCTGATAACAAAA‑AAAGGAACTTCCTGACCCATAGTGAAATCGAATCACTCCTTAAAGCAGCAAATACCGGGCCGCATGCAGCACGTAATTATTg                                                   >  1:2740474/1‑140 (MQ=255)

                                                                                     cTAGTGCGCGTCTGTAATTATAAGGGAAAAACGATGAAGAATAAGGCTGATAACAAAA‑AAAGGAACTTCCTGACCCATAGGGAAATCGAATCACTCCTTAAAGCAGCAAATACCGGGCCTCATGCAGCACGTAATTATTg                                                   >  1:2862004/1‑140 (MQ=255)

                                                                                       aGTGCGCGTCTGTAATTATAAGGGAAAAACGATGAAGAATAAGGCTGATAACAAAA‑AAAGGAACTTCCTGACCCATAGTGAAATCGAATCACTCCTTAAAGCAGCAAATACCGGGCCTCATGCAGCACGTAATTATTGTc                                                 <  2:4975208/140‑1 (MQ=255)

                                                                                         tGCGCGTCTGTAATTATAAGGGAAAAACGATGAAGAATAAGGCTGATAACAAAA‑AAAGGAACTTCCTGACCCATAGTGAAATCGAATCa                                                                                                  <  2:991172/89‑1 (MQ=255)

                                                                                         tGCGCGTCTGTAATTATAAGGGAAAAACGATGAAGAATAAGGCTGATAACAAAA‑AAAGGAACTTCCTGACCCATAGTGAAATCGAATCACTCCTTAAAGCAGCAAATACCGGGCCTCATGCAGCACGTAATTATTGTCTg                                               >  2:3567792/1‑140 (MQ=255)

                                                                                          gcgcGTCTGTAATTATAAGGGAAAAACGATGAAGAATAAGGCTGATAACAAAA‑AAAGGAACTTCCTGACCCATAGTGAAATCGAATCACTCCTTAAAGCAGCAAATACCGGGCCTCATGCAGCACGTAATTATTGTCTGa                                              >  1:4811652/1‑140 (MQ=255)

                                                                                             cgTCTGTAATTATAAGGGAAAAACGATGAAGAATAAGGCTGATAACAAAA‑AAAGGAACTTCCTGACCCATAGTGAAATCGAAt                                                                                                    <  2:4014936/83‑1 (MQ=255)

                                                                                             cgTCTGTAATTATAAGGGAAAAACGATGAAGAATAAGGCTGATAACAAAA‑AAAGGAACTTCCTGACCCATAGTGAAATCGAAt                                                                                                    >  1:4014936/1‑83 (MQ=255)

                                                                                             cgTCTGTAATTATAAGGGAAAAACGATGAAGAATAAGGCTGATAACAAAA‑AAAGGAACTTCCTGACCCATAGTGAAATCGAATCACTCCTTAAAGCAGCAAATACCGGGCCTCATGCAGCACGTAATTATTGTCTGActt                                           >  1:4724898/1‑140 (MQ=255)

                                                                                             cgTCTGTAATTATAAGGGAAAAACGATGAAGAATAAGGCTGATAACAAAA‑AAAGGAACTTCCTGACCCATAGTGAAATCGAATCACTCCTTAAAGCAGCAAATACCGGGCCTCATGCAGCACGTAATTATTGTCTGActt                                           >  1:769940/1‑140 (MQ=255)

                                                                                             cgTCTGTAATTATAAGGGAAAAACGATGAAGAATAAGGCTGATAAC‑AAAGAAAGGAACTTCCGGACCCATAGTGAAATCGAATCACTCCTTAAAGCAGCAAATACCGGGCCTCAGGCAGCACGTAATTATTGTGTGActt                                           >  2:2907330/1‑140 (MQ=255)

                                                                                               tCTGTAATTATAAGGGAAAAACGATGAAGAATAAGGCTGATAACAAAA‑AAAGGAACTTCCTGACCCATAGTGAAATCGAATCACTCCTTAAAGCAGCAAATACCGGGCCTCATGCAGCACGTAATTATTGTCTGActttg                                         >  1:5027900/1‑140 (MQ=255)

                                                                                               tCTGTAATTATAAGGGAAAAACGATGAAGAATAAGGCTGATAACAAAA‑AAAGGAACTTCCTGACCCATAGTGAAATCGAATCACTCCTTAAAGCAGCAAATACCGGGCCTCATGCAGCACGTAATTATTGTCTGActttg                                         >  1:1548267/1‑140 (MQ=255)

                                                                                               tCTGTAATTATAAGGGAAAAACGATGAAGAATAAGGCTGATAACAAAA‑AAAGGAACTTCCTGACCCATAGTGAAATCGAATCACTCCTTAAAGCAGCAAATACCGGGCCTCATGCAGCACGTAATTATTGTCTGActttg                                         >  1:1759632/1‑140 (MQ=255)

                                                                                               tCTGTAATTATAAGGGAAAAACGATGAAGAATAAGGCTGATAACAAAA‑AAAGGAACTTCCTGACCCATAGTGAAATCGAATCACTCCTTAAAGCAGCAAATACCGGGCCTCATGCAGCACGTAATTATTGTCTGActttg                                         <  2:4887911/140‑1 (MQ=255)

                                                                                               tCTGTAATTATAAGGGAAAAACGATGAAGAATAAGGCTGATAACAAAA‑AAAGGAACTTCCTGACCCATAGTGAAATCGAATCACTCCTTAAAGCAGCAAATACCGGGCCTCATGCAGCACGTAATTATTGTCTGActttg                                         >  2:4819924/1‑140 (MQ=255)

                                                                                               tCTGTAATTATAAGGGAAAAACGATGAACAATAAGGCTGATAACAAAA‑AAAGGAACTTCCTGACGCAGAGTGAAAGCGAATCAATCCTTAAAGCAGCAAAAACCAGGCCTCATGCAGCACGTAATTATTGTCTAActttg                                         >  2:1159569/1‑140 (MQ=255)

                                                                                                 tGTAATTATAAGGGAAAAACGATGAAGAATAAGGCTGATAACAAAA‑AAAGGAACTTCCTGACCCATAGTGAAATCGAATCACTCCTTAAAGCAGCAAATACCGGGCCTCATGCAGCACGTAATTATTGTCTGActttgct                                       <  2:2477968/140‑1 (MQ=255)

                                                                                                 tGTAATTATAAGGGAAAAACGATGAAGAATAAGGCTGATAACAAAA‑AAAGGAACTTCCTGACCCATAGTGAAATCGAATCACTCCTTAAAGCAGCAAATACCGGGCCTCATGCAGCACGTAATTATTGTCTGActttgct                                       <  2:2477830/140‑1 (MQ=255)

                                                                                                 tGTAATTATAAGGGAAAAACGATGAAGAATAAAGCTGATAACAAAA‑AAAGGAACTTCCTGACCCATAGTGAAATCGAATCACTCCTTAAAGCAGCAAATACCGGGCCTCATGCAGCACGTAATTATTGGCTGActttgct                                       <  1:4327503/140‑1 (MQ=255)

                                                                                                  gTAATTATAAGGGAAAAACGATGAAGAATAAGGCTGATAACAAAA‑AAAGGAACTTCCTGACCCATAGTGAAATCGAATCACTCCTTAAAGCAGCAAATACCGGGCCTCATGCAGCACGTAATTATTGTCTGActttgctt                                      >  2:1511538/1‑140 (MQ=255)

                                                                                                  gTAATTATAAGGGAAAAACGATGAAGAATAAGGCTGATAACAAAA‑AAAGGAACTTCCTGACCCATAGTGAAATCGAATCACTCCTTAAAGCAGCAAATACCGGGCCTCATGCAGCACGTAATTATTGTCTGActttgctt                                      >  2:1511327/1‑140 (MQ=255)

                                                                                                     aTTATAAGGGAAAAACGATGAAGAATAAGGCTGATAACAAAA‑AAAGGAACTTCCTGACCCATAGTGAAATCGAATCACTCCTTAAAGCAGCAAATACCGGGCCTCATGCAGCACGTAATTATTGTCTGACTTTGCTTTGt                                   <  1:720046/140‑1 (MQ=255)

                                                                                                          aaGGGAAAAACGGTGAAGAAGAAGGCTGATAACAAAA‑AAAGGAACTTCCTGACCCATAGTGAAATCGAATCACTCCTTAAAGCAGCAAATACCGGGCCTCATGCAGCACGTAATTATTGTCTGACTTTGCTTTGTTTTAt                              >  2:5048344/1‑140 (MQ=255)

                                                                                                          aaGGGAAAAACGATGAAGAATAAGGCTGATAACAAAA‑AAAGGAACTTCCTGACCCATAGTGAAATCGAATCACTCCTTAAAGCAGCAAATACCGGGCCTCATGCAGCACGTAATTATTGTCTGACTTTGCTTTGTTTTAt                              >  2:3458188/1‑140 (MQ=255)

                                                                                                          aaGGGAAAAACGATGAAGAATAAGGCTGATAACAAAA‑AAAGGAACTTCCTGACCCATAGTGAAATCGAATCACTCCTTAAAGCAGCAAATACCGGGCCTCATGCAGCACGTAATTATTGTCTGACTTTGCTTTGTTTTAt                              >  1:1769519/1‑140 (MQ=255)

                                                                                                             ggAAAAACGATGAAGAATAAGGCTGATAACAAAA‑AAAGGAACTTCCTGACCCATAGTGAAATCGAATCACTCCTTAAAGCAGCAAATACCGGGCCTCATGCAGCACGTAATTATTGTCTGACTTTGCTTTGTTTTATTCa                           >  2:839243/1‑140 (MQ=255)

                                                                                                             ggAAAAACGATGAAGAATAAGGCTGATAACAAAA‑AAAGGAACTTCCTGACCCATAGTGAAATCGAATCACTCCTTAAAGCAGCAAATACCGGGCCTCATGCAGCACGTAATTATTGTCTGACTTTGCTTTGTTTTATTCa                           <  2:3785144/140‑1 (MQ=255)

                                                                                                             ggAAAAACGATGAAGAATAAGGCTGATAACAAAA‑AAAGGAACTTCCAGACCCATAGTGAAATCGAATCACTCCTTAATGCAGCAAATACCGGGCCTCATGCAGCACGTAATTATTGTCTGACTTTGCTTTGTTTAATTCa                           >  2:4502031/1‑140 (MQ=255)

                                                                                                               aaaaaCGATGAAGAATAAGGCTGATAACAAAA‑AAAGGAACTTCCTGACCCATAGTGAAATCGAATCACTCCTTAAAGCAGCAAATACCGGGCCTCATGCAGCACGTAATTATTGTCTGACTTTGCTTTGTTTTATTCATg                         <  1:1400559/140‑1 (MQ=255)

                                                                                                                aaaaCGATGAAGAATAAGGCTGATAACAAAA‑AAAGGAACTTCCTGACCCATAGTGAAATCGAATCACTCCTTAAAGCAGCAAATACCGGGCCTCATGCAGCACGTAATTATTGTCTGACTTTGCTTTGTTTTATTCATgg                        >  1:3495859/1‑140 (MQ=255)

                                                                                                                 aaaCGATGAAGAATAAGGCTGATAACAAAA‑AAAGGAACTTCCTGACCCATAGTGAAATCGAATCACTCCTTAAAGCAGCAAATACCGGGCCTCATGCAGCACGTAATTATTGTCTGACTTTGCTTTGTTTTATTCATGGt                       >  1:3298506/1‑140 (MQ=255)

                                                                                                                                 gtcTGATAACAAAA‑AAAGGAACTTCCTGACCCATAGTGAAATCGAATCACTCCTTAAAGCAGCAAATACCGGGCCTCATGCAGCACGTAATTATTGTCTGACGTTGCTTTGTTTTATTCATGGTTTCCGGGCGAGTGaaa       >  2:2158215/3‑140 (MQ=255)

                                                                                                                                      aTAACAAAA‑AAAGGACCTTCCTGACCCATAGTGAAATCGAATCACTCCTTAAAGCAGCAAATACCGGGCCTCATGCAGCACGTAATTATTGTCTGACTTTGCTTTGTTTTATTCATGGTTTCCGGGCGAGTGAAATTTGt  <  1:2991137/140‑1 (MQ=255)

                                                                                                                                      aTAACAAAA‑AAAGGAACTTCCTGACCCATAGTGAAATCGAATCACTCCTTAAAGCAGCAAATACCGGGCCTCATGCAGCACGTAATTATTGTCTGACTTTGCTTTGTTTTATTCATGGTTTCCGGGCTAGTGAAAttttt  >  2:2438213/1‑138 (MQ=255)

                                                                                                                                      aTAACAAAA‑AAAGGAACTTCCTGACCCATAGTGAAATCGAATCACTCCTTAAAGCAGCAAATACCGGGCCTCATGCAGCACGTAATTATTGTCTGACTTTGCTTTGTTTTATTCATGGTTTCCGGGCGAGTGAAATTTGt  >  2:4849284/1‑140 (MQ=255)

                                                                                                                                      aTAACAAAA‑AAAGGAACTTCCTGACCCATAGTGAAATCGAATCACTCCTTAAAGCAGCAAATACCGGGCCTCATGCAGCACGTAATTATTGTCTGACTTTGCTTTGTTTTATTCATGGTTTCCGGGCGAGTGAAATTTGt  >  2:2665557/1‑140 (MQ=255)

                                                                                                                                      aTAACAAAA‑AAAGGAACTTCCTGACCCATAGTGAAATCGAATCACTCCTTAAAGCAGCAAATACCGGGCCTCATGCAGCACGTAATTATTGTCTGACTTTGCTTTGTTTTATTCATGGTTTCCGGGCGAGTGAAATTTGt  >  2:4308168/1‑140 (MQ=255)

                                                                                                                                      aTAACAAAA‑AAAGGAACTTCCTGACCCATAGTGAAATCGAATCACTCCTTAAAGCAGCAAATACCGGGCCTCATGCAGCACGTAATTATTGTCTGACTTTGCTTTGTTTTATTCATGGTTTCCGGGCGAGTGAAATTTGt  >  2:787329/1‑140 (MQ=255)

                                                                                                                                      aTAACAAAA‑AAAGGAACTTCCTGACCCATAGTGAAATCGAATCACTCCTTAAAGCAGCAAATACCGGGCCTCATGCAGCACGTAATTATTGTCTGACTTTGCTTTGTTTTATTCATGGTTTCCGGGCGAGTGAAATTTGt  >  2:2909755/1‑140 (MQ=255)

CCACTTACGTTAGTTATTAAGCAAACGTTCGCTTTTATAAACATAATCAGGATAAAAATGTTGGATTATTGCTAACCCAGCACAGCTAGTGCGCGTCTGTAATTATAAGGGAAAAACGATGAAGAATAAGGCTGATAAC‑AAA‑AAAGGAACTTCCTGACCCATAGTGAAATCGAATCACTCCTTAAAGCAGCAAATACCGGGCCTCATGCAGCACGTAATTATTGTCTGACTTTGCTTTGTTTTATTCATGGTTTCCGGGCGAGTGAAATTTGT  >  NZ_CP025268/4542754‑4543026

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

GGTCCACTTACGTTAGTTATTAAGCAAACGTTCGCTTTTATAAACATAATCAGGATAAAAATGTTGGATTATTGCTAACCCAGCACAGCTAGTGCGCGTCTGTAATTATAAGGGAAAAACGATGAAGAATAAGGCTGATAAC‑AAAAAAGGAACTTCCTGACCCATAGTGAAATCGAATCACTCCTTAAAGCAGCAAATACCGGGCCTCATGCAGCACGTAATTATTGTCTGACTTTGCTTTGTTTTATTCATGGTTTCCGGGCGAGTGAAATTTGTCGATTGAGGATTTCG  >  NZ_CP025268/4542751‑4543041

GGTCCACTTACGTTAGTTATTAAGCAAACGTTCGCTTTTATAAACATAATCAGGATAAAAATGTTGGATTATTGCTAACCCAGCACAGCTAGTGCGCGTCTGTAATTATAAGGGAAAAACGATGAAGAATAAGGCTGATAAC‑AAAAAAagg                                                                                                                                              <  SRR14920169.1365681/151‑4 (MQ=60)

     ACTTACGTTAGTTATTAAGCAAACGTTCGCTTTTATAAACATAATCAGGATAAAAATGTTGGATTATTGCTAACCCAGCACAGCTAGTGCGCGTCTGTAATTATAAGGGAAAAACGATGAAGAATAAGGCTGATAACAAAAAAAGGAACTT                                                                                                                                          >  SRR14920169.2262244/1‑151 (MQ=60)

            TTAGTTATTAAGCAAACGTTCGCTTTTATAAACATAATCAGGATAAAAATGTTGGATTATTGCTAACCCAGCACAGCTAGTGCGCGTCTGTAATTATAAGGGAAAAACGATGAAGAATAAGGCTGATAACAAAAAAAGGAACTTCCTGACC                                                                                                                                   <  SRR14920169.695636/151‑1 (MQ=60)

               GTTATTAAGCAAACGTTCGCTTTTATAAACATAATCAGGATAAAAATGTTGGATTATTGCTAACCCAGCACAGCTAGTGCGCGTCTGTAATTATAAGGGAAAAACGATGAAGAATAAGGCTGATAACAAAAAAAGGAACTTCCTGACCCAT                                                                                                                                >  SRR14920169.2513156/1‑151 (MQ=60)

                   TTAAGCAAACGTTCGCTTTTATAAACATAATCAGGATAAAAATGTTGGATTATTGCTAACCCAGCACAGCTAGTGCGCGTCTGTAATTATAAGGGAAAAACGATGAAGAATAAGGCTGATAACAAAAAAAGGAACTTCCTGACCCATAGTG                                                                                                                            <  SRR14920169.5056924/151‑1 (MQ=60)

                     AAGCAAACGTTCGCTTTTATAAACATAATCAGGATAAAAATGTTGGATTATTGCTAACCCAGCACAGCTAGTGCGCGTCTGTAATTATAAGGGAAAAACGATGAAGAATAAGGCTGATAACAAAAAAAGGAACTTCCTGACCCATAGTGAA                                                                                                                          >  SRR14920169.1169934/1‑151 (MQ=60)

                                  CTTTTATAAACATAATCAGGATAAAAATGTTGGATTATTGCTAACCCAGCACAGCTAGTGCGCGTCTGTAATTATAAGGGAAAAACGATGAAGAATAAGGCTGATAACAAAAAAAGGAACTTCCTGACCCATAGTGAAATCGAATCACTCC                                                                                                             >  SRR14920169.5023058/1‑151 (MQ=60)

                                    TTTATAAACATAATCAGGATAAAAATGTTGGATTATTGCTAACCCAGCACAGCTAGTGCGCGTCTGTAATTATAAGGGAAAAACGATGAAGAATAAGGCTGATAACAAAAAAAGGAACTTCCTGACCCATAGTGAAATCGAATCACTCCTT                                                                                                           >  SRR14920169.3498035/1‑151 (MQ=60)

                                       ATAAACATAATCAGGATAAAAATGTTGGATTATTGCTAACCCAGCACAGCTAGTGCGCGTCTGTAATTATAAGGGAAAAACGATGAAGAATAAGGCTGATAACAAAAAAAGGAACTTCCTGACCCATAGTGAAATCGAATCACTCCTTAAA                                                                                                        <  SRR14920169.3159595/151‑1 (MQ=60)

                                       ATAAACATAATCAGGATAAAAATGTTGGATTATTGCTAACCCAGCACAGCTAGTGCGCGTCTGTAATTATAAGGGAAAAACGATGAAGAATAAGGCTGATAACAAAAAAAGGAACTTCCTGACCCATAGTGAAATCGAATCACTCCTTAAA                                                                                                        <  SRR14920169.534289/151‑1 (MQ=60)

                                         AAACATAATCAGGATAAAAATGTTGGATTATTGCTAACCCAGCACAGCTAGTGCGCGTCTGTAATTATAAGGGAAAAACGATGAAGAATAAGGCTGATAACAAAAAAAGGAACTTCCTGACCCATAGTGAAATCGAATCACTCCTTAAAGC                                                                                                      <  SRR14920169.1214721/151‑1 (MQ=60)

                                         AAACATAATCAGGATAAAAATGTTGGATTATTGCTAACCCAGCACAGCTAGTGCGCGTCTGTAATTATAAGGGAAAAACGATGAAGAATAAGGCTGATAACAAAAAAAGGAACTTCCTGACCCATAGTGAAATCGAATCACTCCTTAAAGC                                                                                                      <  SRR14920169.1214780/151‑1 (MQ=60)

                                                  CAGGATAAAAATGTTGGATTATTGCTAACCCAGCACAGCTAGTGCGCGTCTGTAATTATAAGGGAAAAACGATGAAGAATAAGGCTGATAACAAAAAAAGGAACTTCCTGACCCATAGTGAAATCGAATCACTCCTTAAAGCAGCAAATAC                                                                                             <  SRR14920169.3145908/151‑1 (MQ=60)

                                                    GGATAAAAATGTTGGATTATTGCTAACCCAGCACAGCTAGTGCGCGTCTGTAATTATAAGGGAAAAACGATGAAGAATAAGGCTGATAACAAAAAAAGGAACTTCCTGACCCATAGTGAAATCGAATCACTCCTTAAAGCAGCAAATACCG                                                                                           >  SRR14920169.1974509/1‑151 (MQ=60)

                                                    GGATAAAAATGTTGGATTATTGCTAACCCAGCACAGCTAGTGCGCGTCTGTAATTATAAGGGAAAAACGATGAAGAATAAGGCTGATAACAAAAAAAGGAACTTCCTGACCCATAGTGAAATCGAATCACTCCTTAAAGCAGCAAATACCG                                                                                           >  SRR14920169.3842695/1‑151 (MQ=60)

                                                    GGATAAAAATGTTGGATTATTGCTAACCCAGCACAGCTAGTGCGCGTCTGTAATTATAAGGGAAAAACGATGAAGAATAAGGCTGATAACAAAAAAAGGAACTTCCTGACCCATAGTGAAATCGAATCACTCCTTAAAGCAGCAAATACCG                                                                                           >  SRR14920169.3140000/1‑151 (MQ=60)

                                                                TGGATTATTGCTAACCCAGCACAGCTAGTGCGCGTCTGTAATTATAAGGGAAAAACGATGAAGAATAAGGCTGATAACAAAAAAAGGAACTTCCTGACCCATAGTGAAATCGAATCACTCCTTAAAGCAGCAAATACCGGGCCTCATGCAG                                                                               <  SRR14920169.58886/151‑1 (MQ=60)

                                                                TGGATTATTGCTAACCCAGCACAGCTAGTGCGCGTCTGTAATTATAAGGGAAAAACGATGAAGAATAAGGCTGATAACAAAAAAAGGAACTTCCTGACCCATAGTGAAATCGAATCACTCCTTAAAGCAGCAAATACCGGGCCTCATGCAG                                                                               <  SRR14920169.2640201/151‑1 (MQ=60)

                                                                 GGATTATTGCTAACCCAGCACAGCTAGTGCGCGTCTGTAATTATAAGGGAAAAACGATGAAGAATAAGGCTGATAACAAAAAAAGGAACTTCCTGACCCATAGTGAAATCGAATCACTCCTTAAAGCAGCAAATACCGGGCCTCATGCAGC                                                                              >  SRR14920169.209517/1‑151 (MQ=60)

                                                                   ATTATTGCTAACCAAGCACAGCTAGTGCGCGTCTGTAATTATAAGGGAAAAACGATGAAGAATAAGGCTGATAACAAAAAAAGGAACTTCCTGACCCATAGTGAAATCGAATCACTCCTTAAAGCAGCAAATACCGGGCCTCATGCAGCAC                                                                            <  SRR14920169.1903255/151‑1 (MQ=60)

                                                                      ATTGCTAACCCAGCACAGCTAGTGCGCGTCTGTAATTATAAGGGAAAAACGATGAAGAATAAGGCTGATAACAAAAAAAGGAACTTCCTGACCCATAGTGAAATCGAATCACTCCTTAAAGCAGCAAATACCGGGCCTCATGCAGCACGTA                                                                         >  SRR14920169.4117118/1‑151 (MQ=60)

                                                                      ATTGCTAACCCAGCACAGCTAGTGCGCGTCTGTAATTATAAGGGAAAAACGATGAAGAATAAGGCTGATAACAAAAAAAGGAACTTCCTGACCCATAGTGAAATCGAATCACTCCTTAAAGCAGCAAATACCGGGCCTCATGCAGCACGTA                                                                         >  SRR14920169.477024/1‑151 (MQ=60)

                                                                         GCTAACCCAGCACAGCTAGTGCGCGTCTGTAATTATAAGGGAAAAACGATGAAGAATAAGGCTGATAACAAAAAAAGGAACTTCCTGACCCATAGTGAAATCGAATCACTCCTTAAAGCAGCAAATACCGGGCCTCATGCAGCACGTAATT                                                                      >  SRR14920169.694479/1‑151 (MQ=60)

                                                                         GCTAACCCAGCACAGCTAGTGCGCGTCTGTAATTATAAGGGAAAAACGATGAAGAATAAGGCTGATAACAAAAAAAGGAACTTCCTGACCCATAGTGAAATCGAATCACTCCTTAAAGCAGCAAATACCGGGCCTCATGCAGCACGTAATT                                                                      <  SRR14920169.2033378/151‑1 (MQ=60)

                                                                           TAACCCAGCACAGCTAGTGCGCGTCTGTAATTATAAGGGAAAAACGATGAAGAATAAGGCTGATAACAAAAAAAGGAACTTCCTGACCCATAGTGAAATCGAATCACTCCTTAAAGCAGCAAATACCGGGCCTCATGCAGCACGTAATTAT                                                                    <  SRR14920169.3724206/151‑1 (MQ=60)

                                                                             ACCCAGCACAGCTAGTGCGCGTCTGTAATTATAAGGGAAAAACGATGAAGAATAAGGCTGATAACAAAAAAAGGAACTTCCTGACCCATAGTGAAATCGAATCACTCCTTAAAGCAGCAAATACCGGGCCTCATGCAGCACGTAATTATTG                                                                  >  SRR14920169.2631917/1‑151 (MQ=60)

                                                                             ACCCAGCACAGCTAGTGCGCGTCTGTAATTATAAGGGAAAAACGATGAAGAATAAGGCTGATAACAAAAAAAGGAACTTCCTGACCCATAGTGAAATCGAATCACTCCTTAAAGCAGCAAATACCGGGCCTCATGCAGCACGTAATTATTG                                                                  >  SRR14920169.2591697/1‑151 (MQ=60)

                                                                             ACCCAGCACAGCTAGTGCGCGTCTGTAATTATAAGGGAAAAACGATGAAGAATAAGGCTGATAACAAAAAAAGGAACTTCCTGACCCATAGTGAAATCGAATCACTCCTTAAAGCAGCAAATACCGGGCCTCATGCAGCACGTAATTATTG                                                                  <  SRR14920169.974303/151‑1 (MQ=60)

                                                                             ACCCAGCACAGCTAGTGCGCGTCTGTAATTATAAGGGAAAAACGATGAAGAATAAGGCTGATAACAAAAAAAGGAACTTCCTGACCCATAGTGAAATCGAATCACTCCTTAAAGCAGCAAATACCGGGCCTCATGCAGCACGTAATTATTG                                                                  >  SRR14920169.662657/1‑151 (MQ=60)

                                                                             ACCCAGCACAGCTAGTGCGCGTCTGTAATTATAAGGGAAAAACGATGAAGAATAAGGCTGATAACAAAAAAAGGAACTTCCTGACCCATAGTGAAATCGAATCACTCCTTAAAGCAGCAAATACCGGGCCTCATGCAGCACGTAATTATTG                                                                  >  SRR14920169.600213/1‑151 (MQ=60)

                                                                             ACCCAGCACAGCTAGTGCGCGTCTGTAATTATAAGGGAAAAACGATGAAGAATAAGGCTGATAACAAAAAAAGGAACTTCCTGACCCATAGTGAAATCGAATCACTCCTTAAAGCAGCAAATACCGGGCCTCATGCAGCACGTAATTATTG                                                                  <  SRR14920169.2064722/151‑1 (MQ=60)

                                                                             ACCCAGCACAGCTAGTGCGCGTCTGTAATTATAAGGGAAAAACGATGAAGAATAAGGCTGATAACAAAAAAAGGAACTTCCTGACCCATAGTGAAATCGAATCACTCCTTAAAGCAGCAAATACCGGGCCTCATGCAGCACGTAATTATTG                                                                  >  SRR14920169.4616247/1‑151 (MQ=60)

                                                                             ACCCAGCACAGCTAGTGCGCGTCTGTAATTATAAGGGAAAAACGATGAAGAATAAGGCTGATAACAAAAAAAGGAACTTCCTGACCCATAGTGAAATCGAATCACTCCTTAAAGCAGCAAATACCGGGCCGCATGCAGCACGTAATTATTG                                                                  >  SRR14920169.2746557/1‑151 (MQ=60)

                                                                             ACCCAGCACAGCTAGTGCGCGTCTGTAATTATAAGGGAAAAACGATGAAGAATAAGGCTGATAACAAAAAAAGGAACTTCCTGACCCATAGGGAAATCGAATCACTCCTTAAAGCAGCAAATACCGGGCCTCATGCAGCACGTAATTATTG                                                                  >  SRR14920169.2868724/1‑151 (MQ=60)

                                                                             ACCCAGCACAGCTAGTGCGCGCCGGTAATTATAAGGGAAAAACGATGAAGAATAAGGCTGATAACAAAAAAAGGAACTTCCTGACCCATAGTGAAATCGAATCACTCCTTAAAGCAGCAAATACCGGGCgctgtcgcttatacacatctcc                                                                  >  SRR14920169.1453068/1‑129 (MQ=60)

                                                                               CCAGCACAGCTAGTGCGCGTCTGTAATTATAAGGGAAAAACGATGAAGAATAAGGCTGATAACAAAAAAAGGAACTTCCTGACCCATAGTGAAATCGAATCACTCCTTAAAGCAGCAAATACCGGGCCTCATGCAGCACGTAATTATTGTC                                                                >  SRR14920169.2730033/1‑151 (MQ=60)

                                                                                 AGCACAGCTAGTGCGCGTCTGTAATTATAAGGGAAAAACGATGAAGAATAAGGCTGATAACAAAAAAAGGAACTTCCTGACCCATAGTGAAATCGAATCACTCCTTAAAGCctgtctcttatacacatctccgagcccacgagaccggagc                                                              >  SRR14920169.993427/1‑111 (MQ=60)

                                                                                  GCACAGCTAGTGCGCGTCTGTAATTATAAGGGAAAAACGATGAAGAATAAGGCTGATAACAAAAAAAGGAACTTCCTGACCCATAGTGAAATCGAATCACTCCTTAAAGCAGCAAATACCGGGCCTCATGCAGCACGTAATTATTGTCTGA                                                             >  SRR14920169.4823260/1‑151 (MQ=60)

                                                                                     CAGCTAGTGCGCGTCTGTAATTATAAGGGAAAAACGATGAAGAATAAGGCTGATAACAAAAAAAGGAACTTCCTGACCCATAGTGAAATCGAATCACTCCTTAAActgtctcttatacacatctccgagcccacgagaccggagcctatct                                                          >  SRR14920169.4024568/1‑105 (MQ=60)

                                                                                     CAGCTAGTGCGCGTCTGTAATTATAAGGGAAAAACGATGAAGAATAAGGCTGATAACAAAAAAAGGAACTTCCTGACCCATAGTGAAATCGAATCACTCCTTAAAGCAGCAAATACCGGGCCTCATGCAGCACGTAATTATTGTCTGACTT                                                          <  SRR14920169.1338885/151‑1 (MQ=60)

                                                                                     CAGCTAGTGCGCGTCTGTAATTATAAGGGAAAAACGATGAAGAATAAGGCTGATAACAAAAAAAGGAACTTCCTGACCCATAGTGAAATCGAATCACTCCTTAAAGCAGCAAATACCGGGCCTCATGCAGCACGTAATTATTGTCTGACTT                                                          >  SRR14920169.771731/1‑151 (MQ=60)

                                                                                     CAGCTAGTGCGCGTCTGTAATTATAAGGGAAAAACGATGAAGAATAAGGCTGATAACAAAAAAAGGAACTTCCTGACCCATAGTGAAATCGAATCACTCCTTAAAGCAGCAAATACCGGGCCTCATGCAGCACGTAATTATTGTCTGACTT                                                          >  SRR14920169.4736383/1‑151 (MQ=60)

                                                                                       GCTAGTGCGCGTCTGTAATTATAAGGGAAAAACGATGAAGAATAAGGCTGATAACAAAAAAAGGAACTTCCTGACCCATAGTGAAATCGAATCACTCCTTAAAGCAGCAAATACCGGGCCTCATGCAGCACGTAATTATTGTCTGACTTTG                                                        >  SRR14920169.5039837/1‑151 (MQ=60)

                                                                                       GCTAGTGCGCGTCTGTAATTATAAGGGAAAAACGATGAAGAATAAGGCTGATAACAAAAAAAGGAACTTCCTGACCCATAGTGAAATCGAATCACTCCTTAAAGCAGCAAATACCGGGCCTCATGCAGCACGTAATTATTGTCTGACTTTG                                                        >  SRR14920169.2069409/1‑151 (MQ=60)

                                                                                       GCTAGTGCGCGTCTGTAATTATAAGGGAAAAACGATGAAGAATAAGGCTGATAACAAAAAAAGGAACTTCCTGACCCATAGTGAAATCGAATCACTCCTTAAAGCAGCAAATACCGGGCCTCATGCAGCACGTAATTATTGTCTGACTTTG                                                        >  SRR14920169.1763566/1‑151 (MQ=60)

                                                                                       GCTAGTGCGCGTCTGTAATTATAAGGGAAAAACGATGAAGAATAAGGCTGATAACAAAAAAAGGAACTTCCTGACCCATAGTGAAATCGAATCACTCCTTAAAGCAGCAAATACCGGGCCTCATGCAGCACGTAATTATTGTCTGACTTTG                                                        >  SRR14920169.1551730/1‑151 (MQ=60)

                                                                                       GCTAGTGCGCGTCTGTAATTATAAGGGAAAAACGATGAAGAATAAGGCTGATAACAAAAAAAGGAACTTCCTGACCCATAGTGAAATCGAATCACTCCTTAAAGCAGCAAATACCGGGCCTCATGCAGCACGTAATTATTGTCTGACTTTG                                                        >  SRR14920169.148828/1‑151 (MQ=60)

                                                                                                  TCTGTAATTATAAGGGAAAAACGATGAAGAATAAGGCTGATAACAAAAAAAGGAACTTCCTGACCCATAGTGAAATCGAATCACTCCTTAAAGCAGCAAATACCGGGCCTCATGCAGCACGTAATTATTGTCTGACTTTGCTTTGTTTTAT                                             >  SRR14920169.1773480/1‑151 (MQ=60)

                                                                                                    TGTAATTATAAGGGAAAAACGATGAAGAATAAAGCTGATAACAAAAAAAGGAACTTCCTGACCCATAGTGAAATCGAATCACTCCTTAAAGCAGCAAATACCGGGCCTCATGCAGCACGTAATTATTGGCTGACTTTGCTTTGTTTTATTC                                           <  SRR14920169.4337983/151‑1 (MQ=60)

                                                                                                     GTAATTATAAGGGAAAAACGATGAAGAATAAGGCTGATAACAAAAAAAGGAACTTCCTGACCCATAGTGAAATCGAATCACTCCTTAAAGCAGCAAATACCGGGCCTCATGCAGCACGTAATTATTGTCTGACTTTGCTTTGTTTTATTCA                                          >  SRR14920169.487341/1‑151 (MQ=60)

                                                                                                        ATTATAAGGGAAAAACGATGAAGAATAAGGCTGATAACAAAAAAAGGAACTTCCTGACCCATAGTGAAATCGAATCACTCCTTAAAGCAGCAAATACCGGGCCTCATGCAGCACGTAATTATTGTCTGACTTTGCTTTGTTTTATTCATGG                                       >  SRR14920169.3503884/1‑151 (MQ=60)

                                                                                                        ATTATAAGGGAAAAACGATGAAGAATAAGGCTGATAACAAAAAAAGGAACTTCCTGACCCATAGTGAAATCGAATCACTCCTTAAAGCAGCAAATACCGGGCCTCATGCAGCACGTAATTATTGTCTGACTTTGCTTTGTTTTATTCATGG                                       <  SRR14920169.721737/151‑1 (MQ=60)

                                                                                                         TTATAAGGGAAAAACGATGAAGAATAAGGCTGATAACAAAAAAAGGAACTTCCTGACCCATAGTGAAATCGAATCACTCCTTAAAGCAGCAAATACCGGGCCTCATGCAGCACGTAATTATTGTCTGACTTTGCTTTGTTTTATTCATGGT                                      >  SRR14920169.3306253/1‑151 (MQ=60)

                                                                                                                GGAAAAACGATGAAGAATAAGGCTGATAACAAAAAAAGGAACTTCCTGACCCATAGTGAAATCGAATCACTCCTTAAAGCAGCAAATACCGGGCCTCATGCAGCACGTAATTATTGTCTGACTTTGCTTTGTTTTATTCATGGTTTCCGGG                               <  SRR14920169.629423/151‑1 (MQ=60)

                                                                                                                  AAAAACGATGAAGAATAAGGCTGATAACAAAAAAAGGAACTTCCTGACCCATAGTGAAATCGAATCACTCCTTAAAGCAGCAAATACCGGGCCTCATGCAGCACGTAATTATTGTCTGACTTTGCTTTGTTTTATTCATGGTTTCCGGGCG                             <  SRR14920169.1403685/151‑1 (MQ=60)

                                                                                                                           GAAGAATAAGGCTGATAACAAAAAAAGGAACTTCCTGACCCATAGTGAAATCGAATCACTCCTTAAAGCAGCAAATACCGGGCCTCATGCAGCACGTAATTATTGTCTGACTTTGCTTTGTTTTATTCATGGTTTCCGGGCGAGTGAAATT                    >  SRR14920169.5089376/1‑151 (MQ=60)

                                                                                                                              GAATAAGGCTGATAACAAAAAAAGGAACTTCCTGACCCATAGTGAAATCGAATCACTCCTTAAAGCAGCAAATACCGGGCCTCATGCAGCACGTAATTATTGTCTGACTTTGCTTTGTTTTATTCATGGTTTCCGGGCGAGTGAAATTTGT                 >  SRR14920169.3866539/1‑151 (MQ=60)

                                                                                                                              GAATAAGGCTGATAACAAAAAAAGGAACTTCCTGACCCATAGTGAAATCGAATCACTCCTTAAAGCAGCAAATACCGGGCCTCATGCAGCACGTAATTATTGTCTGACTTTGCTTTGTTTTATTCATGGTTTCCGGGCGAGTGAAATTTGT                 >  SRR14920169.707581/1‑151 (MQ=60)

                                                                                                                                         ATAACAAAAAAAGGACCTTCCTGACCCATAGTGAAATCGAATCACTCCTTAAAGCAGCAAATACCGGGCCTCATGCAGCACGTAATTATTGTCTGACTTTGCTTTGTTTTATTCATGGTTTCCGGGCGAGTGAAATTTGTCGATTGAGGAT      <  SRR14920169.2998118/151‑1 (MQ=60)

                                                                                                                                             caAAAAAAGGAACTTCCTGACCCATAGTGAAATCGAATCACTCCTTAAAGCAGCAAATACCGGGCCTCATGCAGCACGTAATTATTGTCTGACTTTGCTTTGTTTTATTCATGGTTTCCGGGCGAGTGAAATTTGTCGATTGAGGATTTCG  >  SRR14920169.932266/3‑151 (MQ=60)

                                                                                                                                             caAAAAAAGGAACTTCCTGACCCATAGTGAAATCGAATCACTCCTTAAAGCAGCAAATACCGGGCCTCATGCAGCACGTAATTATTGTCTGACTTTGCTTTGTTTTATTCATGGTTTCCGGGCGAGTGAAATTTGTCGATTGAGGATTTCG  >  SRR14920169.4611270/3‑151 (MQ=60)

                                                                                                                                             caAAAAAAGGAACTTCCTGACCCATAGTGAAATCGAATCACTCCTTAAAGCAGCAAATACCGGGCCTCATGCAGCACGTAATTATTGTCTGACTTTGCTTTGTTTTATTCATGGTTTCCGGGCGAGTGAAATTTGTCGATTGAGGATTTCG  >  SRR14920169.4609731/3‑151 (MQ=60)

GGTCCACTTACGTTAGTTATTAAGCAAACGTTCGCTTTTATAAACATAATCAGGATAAAAATGTTGGATTATTGCTAACCCAGCACAGCTAGTGCGCGTCTGTAATTATAAGGGAAAAACGATGAAGAATAAGGCTGATAAC‑AAAAAAGGAACTTCCTGACCCATAGTGAAATCGAATCACTCCTTAAAGCAGCAAATACCGGGCCTCATGCAGCACGTAATTATTGTCTGACTTTGCTTTGTTTTATTCATGGTTTCCGGGCGAGTGAAATTTGTCGATTGAGGATTTCG  >  NZ_CP025268/4542751‑4543041

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`