Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A3 F1 I1 R1	255	508.1	22210536	99.3%	22055062	151.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_008463	5,028,201	2 bp→CG	intergenic (‑123/+13)	PA14_RS22975 ← / ← PA14_RS22980	Nramp family divalent metal transporter/ribbon‑helix‑helix domain‑containing protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_008463	5,028,201	0	G	C	99.0%	1925.6 / 5.7	496	intergenic (‑123/+14)	PA14_RS22975/PA14_RS22980	Nramp family divalent metal transporter/ribbon‑helix‑helix domain‑containing protein
Reads supporting (aligned to +/- strand): ref base G (2/3); new base C (229/262); total (231/265)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.73e-01
*	NC_008463	5,028,202	0	T	G	99.0%	1932.5 / 6.2	496	intergenic (‑124/+13)	PA14_RS22975/PA14_RS22980	Nramp family divalent metal transporter/ribbon‑helix‑helix domain‑containing protein
Reads supporting (aligned to +/- strand): ref base T (0/0); major base G (229/262); minor base . (2/3); total (231/265)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 496 total aligned reads displayed.

GCGTCGGCCGGCACCGCCACGCTGTGGCTGACCGCCGAGGGACAGAACGGCGCGGTGGCCCGTGGTGGGCAGGGAGTATTTCACGAGGCGACGCCGGATCGGGGAGGAAGTGCGACCAGCCTAAACGTTTCACCCCGGGTGGGTTCTTCCCTCCCCTCAGGCCTGGGCGCGCTGGCCGACCAGGCTCAGCTCGGCGACCTTCTCGCGCCGCTGCGCCAGGTAGCGGGTGCAACTGACACGCAGGAAGGAGGCGAAGTTGAGCACCTCGCCGCGATAGTCCA  >  NC_008463/5028059‑5028339

||

 cGTCGGCCGGCACCGCCACGCTGTGGCTGACCGCCGAGGGACAGAACGGCGCGGTGG‑CCGTGGTGGGCAGGGAGTATTTCACGAGGCGACGCCGGATCGGGGAGGAAGTGCGACCAGCCTAAACGTTTCACCCC‑GGTCGCGTCTtccctcc                                                                                                                                 <  1:2751040/151‑1 (MQ=255)

  gTCGGCCGGCACCGCCACGCTGTGGCTGACCGCCGAGGGACAGAACGGCGCGGTGG‑CCGTGGTGGGCAGGGAGTATTTCACGAGGCGACGCCGGATCGGGGAGGAAGTGCGACCAGCCTAAACGTTTCACCCC‑GGTCGCGTCTtccctccc                                                                                                                                <  2:837897/151‑1 (MQ=255)

  gTCGGCCGGCACCGCCACGCTGTGGCTGACCGCCGAGGGACAGAACGGCGCGGTGG‑CCGTGGTGGGCAGGGAGTATTTCACGAGGCGACGCCGGATCGGGGAGGAAGTGCGACCAGCCTAAACGTTTCACCCC‑GGTCGCGTCTtccctccc                                                                                                                                >  2:7102965/1‑151 (MQ=255)

   tCGGCCGGCACCGCCACGCTGTGGCTGACCGCCGAGGGACAGAACGGCGCGGTGG‑CCGTGGTGGGCAGGGGGTATTTCACGAGGCGACGCCGGATCGGGGAGGAAGTGCGACCAGCCTAAACGTTTCACCCC‑GGTCGCGTCTTccctcccc                                                                                                                               <  1:144834/151‑1 (MQ=255)

     ggccggcACCGCCACGCTGTGGCTGACCGCCGAGGGACAGAACGGCGCGGTGG‑CCGTGGTGGGCAGGGAGTATTTCACGAGGCGACGCCGGATCGGGGAGGAAGTGCGACCAGCCTAAACGTTTCACCCC‑GGTCGCGTCTTccctcccctc                                                                                                                             >  1:9940675/1‑151 (MQ=255)

     ggccggcACCGCCACGCTGTGGCTGACCGCCGAGGGACAGAACGGCGCGGTGG‑CCGTGGTGGGCAGGGAGTATTTCACGAGGCGACGCCGGATCGGGGAGGAAGTGCGACCAGCCTAAACGTTTCACCCC‑GGTCGCGTCTTccctcccctc                                                                                                                             <  1:1304858/151‑1 (MQ=255)

       ccggcACCGCCACGCTGTGGCTGACCGCCGAGGGACAGAACGGCGCGGTGG‑CCGTGGTGGGCAGGGAGTATTTCACGAGGCGACGCCGGATCGGGGAGGAAGTGCGACCAGCCTAAACGTTTCACCCC‑GGTCGCGTCTTCCCTCCCCTCAg                                                                                                                           >  2:8129646/1‑151 (MQ=255)

        cggcACCGCCACGCTGTGGCTGACCGCCGAGGGACAGAACGGCGCGGTGG‑CCGTGGTGGGCAGGGAGTATTTCACGAGGCGACGCCGGATCGGGGAGGAAGTGCGACCAGCCTAAACGTTTCACCCC‑GGTCGCGTCTTCCCTCCCCTCAgg                                                                                                                          >  1:2179594/1‑151 (MQ=255)

             ccGCCACGCTGTGGCTGACCGCCGAGGGACAGAACGGCGCGGTGG‑CCGTGGTGGGCAGGGCGTAGTGCTCGAGGCGACGCCGGATCGGTGAGGAAGTGCGAACAGCCTAATCGTTTCACCCC‑GGTCGCGTCTTCCCTCCCCTCTGGCCTgg                                                                                                                     >  2:6591208/1‑151 (MQ=255)

             ccGCCACGCTGTGGCTGACCGCCGAGGGACAGAACGGCGCGGTGG‑CCGTGGTGGGCAGGGAGTATTTCACGAGGCGACGCCGGATCGGGGAGGAAGTGCGACCAGCCTAAACGTTTCACCCC‑GGTCGCGTCTTCCCTCCCCTCAGGCCTgg                                                                                                                     <  2:4516698/151‑1 (MQ=255)

              cGCCACGCTGTGGCTGACCGCCGAGGGACAGAACGGCGCGGTGG‑CCGTGGTGGGCAGGGAGTATTTCACGAGGCGACGCCGGATCGGGGAGGAAGTGCGACCAGCCTAAACGTTTCACCCC‑GGTCGCGTCTTCCCTCCCCTCAGGCCTggg                                                                                                                    <  1:5472428/151‑1 (MQ=255)

                  aCGCTGTGGCTGACCGCCGAGGGACAGAACGGCGCGGTGG‑CCGGGGTGGGCAGGGAGTATTTCTCGAGGCGACGGCGGATCGGGGAGGAAGTGCGACAAGCCTAAACGTTTCACCCC‑GGTCGCGTCTTCCCTCCCCTCAGGCCGGGgcgcg                                                                                                                >  2:10912366/1‑151 (MQ=255)

                   cGCTGTGGCTGACCGCCGAGGGACAGAACGGCGCGGTGG‑CCGTGGTGGGCAGGGAGTATTTCACGAGGCGACGCCGGATCGGGGAGGAAGTGCGACCAGCCTAAACGTTTCACCCC‑GGTCGCGTCTTCCCTCCCCTCAGGCCTGGgcgcgc                                                                                                               >  1:4640590/1‑151 (MQ=255)

                    gCTGTGGCTGACCGCCGAGGGACAGAACGGCGCGGTGG‑CCGTGGTGGGCAGGGAGTATTTCACGAGGCGACGCCGGATCGGGGAGGAAGTGCGACCAGCCTAAACGTTTCACCCC‑GGTCGCGTCTTCCCTCCCCTCAGGCCTGGGCGCGCt                                                                                                              <  1:4870108/151‑1 (MQ=255)

                    gCTGTGGCTGACCGCCGAGGGACAGAACGGCGCGGTGG‑CCGGGGAGGGCAGGGAGTATTTCAAGAGGCGACGCAGGATCGGGGAGGTAGTGCGACCAGCGTAAAAGTTTCACCCC‑GGTCGCGTCTTCCCTCCCCTCAGGCCGGGgcgcggt                                                                                                              >  2:7563017/1‑149 (MQ=255)

                      tgtgGCTGACCGCCGAGGGACAGAACGGCGCGGTGG‑CCGTGGTGGGCAGGGAGTATTTCACGAGGCGACGCCGGATCGGGGAGGAAGTGCGACCAGCCTAAACGTTTCACCCC‑GGTCGCGTCTTCCCTCCCCTCAGGCCTGGGCGCGCTgg                                                                                                            >  1:3895762/1‑151 (MQ=255)

                      tgtgGCTGACCGCCGAGGGACAGAACGGCGCGGTGG‑CCGTGGGGGGCAGGGAGTATTTCTCGAGGCGACGCCGGATCGGGGTGGAAGTGCGACCAGGCTCAACGTTTCAACCCGGGCGCG‑GCTTCCCTCCGCGCGGGCCTGGGCGCGCTgg                                                                                                            >  2:1988235/1‑151 (MQ=255)

                        tgGCTGACCGCCGAGGGACAGAACGGCGCGGTGG‑CCGTGGTGGGCAGGGAGTATTTCACGAGGCGACGCCGGATCGGGGAGGAAGTGCGACCAGCCTAAACGTTTCACCCC‑GGTCGCGTCTTCCCTCCCCTCAGGCCTGGGCGCGCTGGcc                                                                                                          <  1:10982296/151‑1 (MQ=255)

                            tGACCGCCGAGGGACAGAACGGCGCGGTGG‑CCGTGGTGGGCAGGGAGTATTTCACGAGTCGACGCCGGATCGGGGAGGAAGTGCGACCAGCCTAAACGTTTCACCCC‑GGTCGCGTCTTCCCTCCCCTCAGGCCTGGGCGCGCTGGCCGAcc                                                                                                      >  1:10339765/1‑151 (MQ=255)

                            tGACCGCCGAGGGACAGAACGGCGCGGTGG‑CCGTGGTGGGCAGGGAGTATTTCACGAGGCGACGCCGGATCGGGGAGGAAGTGCGACCAGCCTAAACGTTTCACCCC‑GGTCGCGTCTTCCCTCCCCTCAGGCCTGGGCGCGCTGGCCGAcc                                                                                                      >  2:5376608/1‑151 (MQ=255)

                            ggACCGCCGCGGGACAGACCGGCGCGGTGG‑CCGGGGTGGGCAGGGAGTATTTCACGAGGCGACGCCGGATCGGGGAGGAAGGGCGACCAGCCTAAACGTTTCACCCC‑GGTCGCGTCTTCCCTCCCCTCAGGCCTGGGCGCGCTGGCCGAcc                                                                                                      <  2:10626959/150‑1 (MQ=255)

                             gACCGCCGAGGGACAGAACGGCGCGGTGG‑CCGTGGTGGGCAGGGAGTATTTCACGAGGCGACGCCGGATCGGGGAGGAAGTGCGACCAGCCTAAACGTTTCACCCC‑GGTCGCGTCTTCCCTCCCCTCAGGCCTGGGCGCGCTGGCCGACCa                                                                                                     <  1:9489883/151‑1 (MQ=255)

                               ccgccgAGGGACAGCACGGCGCGGTGG‑CCGTGGTGGGCAGGGAGTATTTCTCGAGGCGACGCCGGATCGGGGAGGAAGTGCGACCAGCCTAAACGTTTCACCCC‑GGTCGCGTCTTCCCTCCCCACAGGCCTGGGCGCGCTGGCCGACCAgg                                                                                                   <  1:3172379/151‑1 (MQ=255)

                                cgccgAGGGACAGAACGGCGCGGTGG‑CCGTGGTGGGCAGGGAGTATTTCACGAGGCGACGCCGGATCGGGGAGGAAGTGCGACCAGCCTAAACGTTTCACCCC‑GGTCGCGTCTTCCCTCCCCTCAGGCCTGGGCGCGCTGGCCGACCAGGc                                                                                                  >  1:7002400/1‑151 (MQ=255)

                                   cgAGGGACAGAACGGCGCGGTGG‑CCGTGGTGGGCAGGGAGTATTTCACGAGGCGACGCCGGATCGGGGAGGAAGTGCGACCAGCCTAAACGTTTCACCCC‑GGTCGCGTCTTCCCTCCCCTCAGGCCTGGGCGCGCTGGCCGACCAGGCTCa                                                                                               <  1:3037594/151‑1 (MQ=255)

                                     aGGGACAGAACGGCGCGGTGG‑CCGTGGTGGGCAGGGAGTATTTCACGAGGCGACGCCGGATCGGGGAGGAAGTGCGACCAGCCTAAACGTTTCACCCC‑GGTCGCGTCTTCCCTCCCCTCAGGCCTGGGCGCGCTGGCCGACCAGGCTCAGc                                                                                             >  2:1177974/1‑151 (MQ=255)

                                       ggACAGAACGGCGCGGTGG‑CCGTGGTGGGCAGGGAGTATTTCACGAGGCGACGCCGGATCGGGGAGGAAGTGCGACCAGCCTAAACGTTTCACCCC‑GGTCGCGTCTTCCCTCCCCTCAGGCCTGGGCGCGCTGGCCGACCAGGCTCAGCTc                                                                                           <  2:5832139/151‑1 (MQ=255)

                                        gACAGAACGGCGCGGTGG‑CCGTGGTGGGCAGGGAGTATTTCACGAGGCGACGCCGGATCGGGGAGGAAGTGCGACCAGCCTAAACGTTTCACCCC‑GGTCGCGTCGTCCCTCCCCTCAGGCCTGGGCGCGCTGGCCGACCAGGCTCAGCTCg                                                                                          >  1:971353/1‑151 (MQ=255)

                                          cAGAACGGCGCGGTGG‑CCGTGGTGGGCCGGGAGTATTTCACGAGGCGACGCCGGATCGGGGAGGAAGTGCGACCAGCCTAAACGTTTCACCCC‑GGTCGCGTCTTCCCTCCCCTCAGGCCTGGGCGCGCTGGCCGACCAGGCTCAGCTCGGc                                                                                        >  2:3844552/1‑151 (MQ=255)

                                          cAGAACGGCGCGGTGG‑CCGTGGTGGGCAGGGAGTATTTCACGAGGCGACGCCGGATCGGGGAGGAAGTGCGACCAGCCTAAACGTTTCACCCC‑GGTCGCGTCTTCCCTCCCCTCAGGCCTGGGCGCGCTGGCCGACCAGGCTCAGCTCGGc                                                                                        <  1:10454283/151‑1 (MQ=255)

                                           aGAACGGCGCGGTGG‑CCGTGGTGGGCAGGGAGTATTTCACGAGGCGACGCCGGATCGGGGAGGAAGTGCGACCAGCCTAAACGTTTCACCCC‑GGTCGCGTCTTCCCTCCCCTCAGGCCTGGGCGCGCTGGCCGACCAGGCTCAGCTCGGCg                                                                                       >  2:6148729/1‑151 (MQ=255)

                                           aGAACGGCGCGGTGG‑CCGTGGTGGGCAGGGAGTATTTCACGAGGCGACGCCGGATCGGGGAGGAAGTGCGACCAGCCTAAACGTTTCACCCC‑GGTCGCGTCTTCCCTCCCCTCAGGCCTGGGCGCGCTGGCCGACCAGGCTCAGCTCGGCg                                                                                       <  2:4887077/151‑1 (MQ=255)

                                               cGGCGCGGTGG‑CCGTGGTGGGCAGGGAGTATTTCACGAGGCGACGCCGGATCGGGGAGGAAGTGCGACCAGCCTAAACGTTTCACCCC‑GGTCGCGTCTTCCCTCCCCTCAGGCCTGGGCGCGCTGGCCGACCAGGCTCAGCTCGGCGACCt                                                                                   <  1:7105929/151‑1 (MQ=255)

                                                ggCGCGGTGG‑CCGTGGTGGGCAGGGAGTATTTCACGAGGCGACGCCGGATCGGGGAGGAAGTGCGACCAGCCTAAACGTTTCACCCC‑GGTCGCGTCTTCCCTCCCCTCAGGCCTGGGCGCGCTGGCCGACCAGGCTCAGCTCGGCGACCtt                                                                                  <  2:6634727/151‑1 (MQ=255)

                                                ggCGCGGTGG‑CCGTGGTGGGCAGGGAGTATTTCACGAGGCGACGCCGGATCGGGGAGGAAGTGCGACCAGCCTAAACGTTTCACCCC‑GGTCGCGTCTTCCCTCCCCTCAGGCCTGGGCGCGCTGGCCGACCAGGCTCAGCTCGGCGACCtt                                                                                  >  1:1626437/1‑151 (MQ=255)

                                                   gcgGTGG‑CCGTGGTGGGCAGGGAGTATTTCACGAGGCGACGCCGGATCGGGGAGGAAGTGCGACCAGCCTAAACGTTTCACCCC‑GGTCGCGTCTTCCCTCCCCTCAGGCCTGGGCGCGCTGGCCGACCAGGCTCAGCTCGGCGACCTtctc                                                                               <  1:8571498/151‑1 (MQ=255)

                                                   gcgGTGG‑CCGTGGTGGGCAGGGAGTATTTCACGAGGCGACGCCGGATCGGGGAGGAAGTGCGACCAGCCTAAACGTTTCACCCC‑GGTCGCGTCTTCCCTCCCCTCAGGCCTGGGCGCGCTGGCCGACCAGGCTCAGCTCGGCGACCTtctc                                                                               <  2:4314571/151‑1 (MQ=255)

                                                    cgGTGG‑CCGTGGTGGGCAGGGAGTATTTCACGAGGCGACGCCGGATCGGGGAGGAAGTGCGACCAGCCTAAACGTTTCACCCC‑GGTCGCGTCTTCCCTCCCCTCAGGCCTGGGCGCGCTGGCCGACCAGGCTCAGCTCGGCGACCTTCTcg                                                                              <  2:4756368/151‑1 (MQ=255)

                                                     ggTGG‑CCGTGGTGGGCAGGGAGTATTTCACGAGGCGACGCCGGATCGGGGAGGAAGTTCGACCAGCCTAAACGTTTCACCCC‑GGTCGCGTCTTCCCTCCCCTCAGGCCTGGGCGCGCTGGCCGACCAGGCTCAGCTCGGCGACCTTCTcgc                                                                             <  2:9581498/151‑1 (MQ=255)

                                                     ggTGG‑CCGTGGTGGGCAGGGAGTATTTCACGAGGCGACGCCGGATCGGGGAGGAAGTGCGACCAGCCTAAACGTTTCACCCC‑GGTCGCGTCTTCCCTCCCCTCAGGCCTGGGCGCGCTGGCCGACCAGGCTCAGCTCGGCGACCTTCTcgc                                                                             <  1:3752510/151‑1 (MQ=255)

                                                        tggccGTGGTGGGCAGGGAGTATTTCACGAGGCGACGCCGGATCGGGGAGGAAGTGCGACCAGCCTAAACGTTTCACCCC‑GGTCGCGTCTTCCCTCCCCTCAGGCCTGGGCGCGCTGGCCGACCAGGCTCAGCTCGGCGACCTTCTCgcgc                                                                           >  2:5060076/4‑151 (MQ=255)

                                                        tggccGTGGTGGGCAGGGAGTATTTCACGAGGCGACGCCGGATCGGGGAGGAAGTGCGACCAGCCTAAACGTTTCACCCC‑GGTCGCGTCTTCCCTCCCCTCAGGCCTGGGCGCGCTGGCCGACCAGGCTCAGCTCGGCGACCGTCTCgcgc                                                                           >  2:5701893/4‑151 (MQ=255)

                                                         ggccGTGGTGGGCAGGGAGTATTTCACGAGGCGACGCCGGATCGGGGAGGAAGTGCGACCAGCCTAAACGTTTCACCCC‑GGTCGCGTCTTCCCTCCCCTCAGGCCTGGGCGCGCTGGCCGACCAGGCTCAGCTCGGCGACCTTCTCGcgcc                                                                          >  2:770564/3‑151 (MQ=255)

                                                          gccGTGGTGGGCAGGGAGTATTTCACGAGGCGACGCCGGATCGGGGAGGAAGTGCGACCAGCCTAAACGTTTCACCCC‑GGTCGCGTCTTCCCTCCCCTCAGGCCTGGGCGCGCTGGCCGACCAGGCTCAGCTCGGCGACCTTCTCGcgccg                                                                         >  2:9258125/2‑151 (MQ=255)

                                                           ccGTGGTGGGCAGGGAGTATTTCACGAGGCGACGCCGGATCGGGGAGGAAGTGCGACCAGCCTAAACGTTTCACCCC‑GGTCGCGTCTTCCCTCCCCTCCGGCCTGGGCGCGCTGGCCGACCAGGCTCAGCTCGGCGACCTTCTCGcgccgc                                                                        >  2:5926731/1‑151 (MQ=255)

                                                           ccGTGGTGGGCAGGGAGTATTTCACGAGGCGACGCCGGATCGGGGAGGAAGTGCGACCAGCCTAAACGTTTCACCCC‑GGTCGCGTCTTCCCTCCCCTCAGGCCTGGGCGCGCTGGCCGACCAGGCTCAGCTCGGCGACCTTCTCGcgccgc                                                                        >  1:7763742/1‑151 (MQ=255)

                                                            cGTGGTGGGCAGGGAGTATTTCACGAGGCGACGCCGGATCGGGGAGGAAGTGCGACCAGCCTAAACGTGTCACCCC‑GGTCGCGTCTTCCCTCCCCTCAGGCCTGGGCGCGCTGGCCGACCAGGCTCAGCTCGGCGACCTTCTCGCGCCGCt                                                                       <  2:2727045/151‑1 (MQ=255)

                                                              tggtggGCAGGGAGTATTTCACGAGGCGACGCCGGATCGGGGAGGAAGTGCGACCAGCCTAAACGTTTCACCCC‑GGTCGCGTCTTCCCTCCCCTCAGGCCTGGGCGCGCTGGCCGACCAGGCTCAGCTCGGCGACCTTCTCGCGCCGCTgc                                                                     <  2:1539993/151‑1 (MQ=255)

                                                               ggtggGCAGGGAGTATTTCACGAGGCGACGCCGGATCGGGGAGGAAGTGCGACCAGCCTAAACGTTTCACCCC‑GGTCGCGTCTTCCCTCCCCTCAGGCCTGGGCGCGCTGGCCGACCAGGCTCAGCTCGGCGACCTTCTCGCGCCGCTgcg                                                                    >  2:6839388/1‑151 (MQ=255)

                                                                gtggGCAGGGAGTATTTCACGAGGCGACGCCGGATCGGGGAGGAAGTGCGACCAGCCTAAACGTTTCACCCC‑GGTCGCGTCTTCCCTCCCCTCAGGCCTGGGCGCGCTGGCCGACCAGGCTCAGCTCGGCGACCTTCTCGCGCCGCTgcgc                                                                   <  1:3366143/151‑1 (MQ=255)

                                                                  gggCAGGGAGTATTTCACGAGGCGACGCCGGATCGGGGAGGAAGTGCGACCAGCCTAAACGTTTCACCCC‑GGTCGCGTCTTCCCTCCCCTCAGGCCTGGGCGCGCTGGCCGACCAGGCTCAGCTCGGCGACCTTCTCGCGCCGCTGCGCCa                                                                 >  1:7299245/1‑151 (MQ=255)

                                                                  gggCAGGGAGTATTTCACGAGGCGACGCCGGATCGGGGAGGAAGTGCGACCAGCCTAAACGTTTCACCCC‑GGTCGCGTCTTCCCTCCCCTCAGGCCTGGGCGCGCTGGCCGACCAGGCTCAGCTCGGCGACCTTCTCGCGCCGCTGCGCCa                                                                 <  1:10635789/151‑1 (MQ=255)

                                                                   ggCAGGGAGTATTTCACGAGGCGACGCCGGATCGGGGAGGAAGTGCGACCAGCCTAAACGTTTCACCCC‑GGTCGCGTCTTCCCTCCCCTCAGGCCTGGGCGCGCTGGCCGACCAGGCTCAGCTCGGCGACCTTCTCGCGCCGCTGCGCCAg                                                                >  2:8453178/1‑151 (MQ=255)

                                                                   ggCAGGGAGTATTTCACGAGGCGACGCCGGATCGGGGACGAAGTGCGACCAGCCTAAACGTTTCACCCC‑GGTCGCGTCTTCCCTCCCCTCAGGCCTGGGCGCGCTGGCCGACCAGGCTCAGCTCGGCGACCTTCTCGCGCCGCTGCGCCAg                                                                >  1:10860191/1‑151 (MQ=255)

                                                                    gCAGGGAGTATTTCACGAGGCGACGCCGGATCGGGGAGGAAGTGCGACCAGCCTAAACGTTTCACCCC‑GGTCGCGTCTTCCCTCCCCTCAGGCCTGGGCGCGCTGGCCGACCAGGCTCAGCTCGGCGACCTTCTCGCGCCGCTGCGCCAgg                                                               <  1:8325501/151‑1 (MQ=255)

                                                                      aGGGAGTATTTCACGAGGCGACGCCGGATCGGGGAGGAAGTGCGACCAGCCTAAACGTTTCACCCC‑GGTCGCGTCTTCCCTCCCCTCAGGCCTGGGCGCGCTGGCCGACCAGGCTCAGCTCGGCGACCTTCTCGCGCCGCTGCGCCAGGTa                                                             <  2:3285772/151‑1 (MQ=255)

                                                                       gggAGTATTTCACGAGGCGACGCCGGATCGGGGAGGAAGTGCGACCAGCCTAAACGTTTCACCCC‑GGTCGCGTCTTCCCTCCCCTCAGGCCTGGGCGCGCTGGCCGACCAGGCTCAGCTCGGCGACCTTCTCGCGCCGCTGCGCCAGGTAg                                                            <  1:2615797/151‑1 (MQ=255)

                                                                            tATTTCACGAGGCGACGCCGGATCGGGGAGGAAGTGCGACCAGCCTAAACGTTTCACCCC‑GGTCGCGTCTTCCCTCCCCTCAGGCCTGGGCGCGCTGGCCGACCAGGCTCAGCTCGGCGACCTTCTCGCGCCGCTGCGCCAGGTAGCGGGt                                                       >  2:10758846/1‑151 (MQ=255)

                                                                               ttCACGAGGCGACGCCGGATCGGGGAGGAAGTGCGACCAGCCTAAACGTTTCACCCC‑GGTCGCGTCTTCCCTCCCCTCAGGCCTGGGCGCGCTGGCCGACCAGGCTCAGCTCGGCGACCTTCTCGCGCCGCTGCGCCAGGTAGCGGGTGCa                                                    <  1:2892045/151‑1 (MQ=255)

                                                                                tCACGAGGCGACGCCGGATCGGGGAGGAAGTGCGACCAGCCTAAACGTTTCACCCC‑GGTCGCGTCTTCCCTCCCCTCAGGCCTGGGCGCGCTGGCCGACCAGGCTCAGCTCGGCGACCTTCTCGCGCCGCTGCGCCAGGTAGCGGGTGCaa                                                   <  2:10113275/151‑1 (MQ=255)

                                                                                 cACGAGGCGACGCCGGATCGGGGAGGAAGTGCGACCAGCCTAAACGTTTCACCCC‑GGTCGCGTCTTCCCTCCCCTCAGGCCTGGGCGCGCTGGCCGACCAGGCTCAGCTCGGCGACCTTCTCGCGCCGCTGCGCCAGGTAGCGGGTGCacc                                                  >  1:8038700/1‑149 (MQ=255)

                                                                                    gAGGCGACGCCGGATCGGGGAGGAAGTGCGACCAGCCTAAACGTGTCACCCC‑GGTCGCGTCGTCCCTCCCCTCAGGCCGGGGCGCGCTGGCCGACCCGGCGGAGCTCGGCGCCCTTCTCGCGCCGCTGCGCGAGGGCGCGGGGGCAACGGa                                               >  2:940729/1‑151 (MQ=255)

                                                                                       gCGACGCCGGATCGGGGAGGAAGTGCGACCAGCCTACACGTTGCACCCC‑GGTCGCGTCTTCCCTCCCCTCAGGCCTGGGCGCGCTGGCCGACCAGGCTCAGCTCGGCGACCTTCTCGCGCCGCTGCGCCAGGTAGCGGGTGCAACTGacac                                            <  2:1296568/151‑1 (MQ=255)

                                                                                         gACGCCGGATCGGGGAGGAAGTGCGACCAGCCTAAACGTTTCACCCC‑GGTCGCGTCTTCCCTCCCCTCAGGCCTGGGCGCGCTGGCCGACCAGGCTCAGCTCGGCGACCTTCTCGCGCCGCTGCGCCAGGTAGCGGGTGCAACTGACACGc                                          >  1:5761357/1‑151 (MQ=255)

                                                                                         gACGCCGGATCGGGGAGGAAGTGCGACCAGCCTAAACGTTTCACCCC‑GGTCGCGTCTTCCCTCCCCTCAGGCCTGGGCGCGCTGGCCGACCAGGCTCAGCTCGGCGACCTTCTCGCGCCGCTGCGCCAGGTAGCGGGTGCAACTGACACGc                                          <  1:7923486/151‑1 (MQ=255)

                                                                                          aCGCCGGATCGGGGAGGAAGTGCGACCAGCCTAAACGTTTCACCCC‑GGTCGCGTCTTCCCTCCCCTCAGGCCTGGGCGCGCTGGCCGACCAGGCTCAGCTCGGCGACCTTCTCGCGCCGCTGCGCCAGGTAGCGGGTGCAACTGACACGCa                                         >  1:6746114/1‑151 (MQ=255)

                                                                                          aCGCCGGATCGGGGAGGAAGTGCGACCAGCCTAAACGTTTCACCCC‑GGTCGCGTCTTCCCTCCCCTCAGGCCTGGGCGCGCTGGCCGACCAGGCTCAGCTCGGCGACCTTCTCGCGCCGCTGCGCCAGGTAGCGGGTGCAACTGACACGCa                                         <  1:8250266/151‑1 (MQ=255)

                                                                                            gCCGGATCGGGGAGGAAGTGCGACCAGCCTAAACGTTTCACCCC‑GGTCGCGTCTTCCCTCCCCTCAGGCCTGGGCGCGCTGGCCGACCAGGCTCAGCTCGGCGACCTTCTCGCGCCGCTGCGCCAGGTAGCGGGTGCAACTGACACGCagg                                       <  1:10032921/151‑1 (MQ=255)

                                                                                              cGGATCGGGGAGGAAGTGCGACCAGCCTAAACGTTTCACCCC‑GGTCGCGTCTTCCCTCCCCTCAGGCCTGGGCGCGCTGGCCGACCAGGCTCAGCTCGGCGACCTTCTCGCGCCGCTGCGCCAGGTAGCGGGTGCAACTGACACGCaggaa                                     >  2:7046898/1‑151 (MQ=255)

                                                                                               ggATCGGGGAGGAAGTGCGACCAGCCTAAACGTTTCACCCC‑GGTCGCGTCTTCCCTCCCCTCAGGCCTGGGCGCGCTGGCCGACCAGGCTCAGCTCGGCGACCTTCTCGCGCCGCTGCGCCAGGTAGCGGGTGCAACTGACACGCaggaag                                    >  1:4480435/1‑151 (MQ=255)

                                                                                                 aTCGGGGAGGAAGTGCGACCAGCCTAAACGTTTCACCCC‑GGTCGCGTCTTCCCTCCCCTCAGGCCTGGGCGCGCTGGCCGACCAGGCTCAGCTCGGCGACCTTCTCGCGCCGCTGCGCCAGGTAGCGGGTGCAACTGACACGCaggaagga                                  >  1:9396841/1‑151 (MQ=255)

                                                                                                  tCGGGGAGGAAGTGCGACCAGCCTAAACGTTTCACCCC‑GGTCGCGTCTTCCCTCCCCTCAGGCCTGGGCGCGCTGGCCGACCAGGCTCAGCTCGGCGACCTTCTCGCGCCGCTGCGCCAGGTAGCGGGTGCAACTGACACGCAGGAaggag                                 <  1:8139799/151‑1 (MQ=255)

                                                                                                  tCGGGGAGGAAGTGCGACCAGCCTAAACGTTTCACCCC‑GGTCGCGTCTTCCCTCCCCTCAGGCCTGGGCGCGCTGGCCGACCAGGCTCAGCTCGGCGACCTTCTCGCGCCGCTGCGCCAGGTAGCGGGTGCAACTGACACGCAGGAaggag                                 <  1:6370399/151‑1 (MQ=255)

                                                                                                  tCGGGGAGGAAGTGCGACCAGCCTAAACGTTTCACCCC‑GGTCGCGTCTTCCCTCCCCTCAGGCCTGGGCGCGCTGGCCGACCAGGCTCAGCTCGGCGACCTTCTCGCGCCGCTGCGCCAGGTAGCGGGTGCAACTGACACGCAGGAaggag                                 >  1:6927/1‑151 (MQ=255)

                                                                                                  tCGGGGAGGAAGTGCGACCAGCCTAAACGTTTCACCCC‑GGTCGCGTCTTCCCTCCCCTCAGGCCTGGGCGCGCTGGCCGACCAGGCTCAGCTCGGCGACCTTCTCGCGCCGCTGCGCCAGGTAGCGGGTGCAACTGACACGCAGGAaggag                                 <  1:8698084/151‑1 (MQ=255)

                                                                                                   cGGGGAGGAAGTGCGACCAGCCTAAACGTTTCACCCC‑GGTCGCGTCTTCCCTCCCCTCAGGCCTGGGCGCGCTGGCCGACCAGGCTCAGCTCGGCGACCTTCTCGCGCCGCTGCGCCAGGTAGCGGGTGCAACTGACACGCAGGAaggagg                                >  1:5207673/1‑151 (MQ=255)

                                                                                                    ggggAGGAAGTGCGACCAGCCTAAACGTTTCACCCC‑GGTCGCGTCTTCCCTCCCCTCAGGCCGGGGCGCGCTGGCCGACCAGGCTCAGCTCGGCGACCTTCTCGCGCCGCTGCGCCAGGTAGCGGGTGCAACTGACACGCAGGAAGGAGGc                               <  2:1740258/151‑1 (MQ=255)

                                                                                                      ggtggaAGTGCGACCAGCCTAAACGTTTCACGCC‑GGTCGCGTCTTCCCGCCCCTCCGGCCTGGGCGAGCTGGCCGACCAGGCGCAGCGCGGCGGCCTTCTCGCGCCGCTGCGCCAGGGCGCGGGTGCAACTGACACGCTGGTCGgcggcgg                             >  2:3224162/4‑150 (MQ=255)

                                                                                                        aggaAGTGCGACCAGCCTAAACGTTTCACCCC‑GGTCGCGTCTTCCCTCCCCTCAGGCCTGGGCGCGCTGGCCGACCAGGCTCAGCTCGGCGACCTTCTCGCGCCGCTGCGCCAGGTAGCGGGTGCAACTGACACGCAGGAAGGAGGCGAAg                           >  2:2537803/1‑151 (MQ=255)

                                                                                                        aggaAGTGCGACCAGCCTAAACGTTTCACCCC‑GGTCGCGTCTTCCCTCCCCTCAGGCCTGGGCGCGCTGGCCGACCAGGCTCAGCTCGGCGACCTTCTCGCGCCGCTGCGCCAGGTAGCGGGTGCAACTGACACGCAGGAAGGAGGCGAAg                           >  2:5577655/1‑151 (MQ=255)

                                                                                                        aggaAGTGCGACCAGCCTAAACGTTT‑AACCCAGGTCGCGTCTTCCCTCCCCTCAGGCCTGGGCGAGCTGGCCGACCAGGCTCAGCTCGGCGACCTTCTCGCGCCGATGAGCCAGGTAGCGGGTGCAACAGACACGCAGGAAGGAGGCGacg                           >  2:9617057/1‑149 (MQ=255)

                                                                                                           aaGTGCGACCAGCCTAAACGTTTCACCCC‑GGTCGCGTCTTCCCTCCCCTCAGGCCTGGGCGCGCTGGCCGACCAGGCTCAGCTCGGCGACCTTCTCGCGCCGCTGCGCCAGGTAGCGGGTGCAACTGACACGCAGGAAGGAGGCGAAGTTg                        >  1:60052/1‑151 (MQ=255)

                                                                                                             gTGCGACCAGCCTAAACGTTTCACCCC‑GGTCGCGTCTTCCCTCCCCTCAGGCCTGGGCGCGCTGGCCGACCAGGCTCAGCTCGGCGACCTTCTCGCGCCGCTGCGCCAGGTAGCGGGTGCAACTGACACGCAGGAAGGAGGCGAAGTTGAg                      >  2:8765412/1‑151 (MQ=255)

                                                                                                              tGCGACCAGCCTAAACGTTTCACCCCGGGCGCG‑TCTTCCCTCCCCTCAGGCCTGGGCGCGCTGGCCGACCAGGCTCAGCTCGGCGACCTTCTCGCGCCGCTGCGCCAGGTAGCGGGTGCAACTGACACGCAGGAAGGAGGCGAAGTTGAGc                     <  2:10362974/151‑1 (MQ=255)

                                                                                                              tGCGACCAGCCTAAACGTTTCACCCC‑GGTCGCGTCTTCCCTCCCCTCAGGCCTGGGCGCGCTGGCCGACCAGGCTCAGCTCGGCGACCTTCTCGCGCCGCTGCGCCAGGTAGCGGGTGCAACTGACACGCAGGAAGGAGGCGAAGTTGAGc                     <  2:4003147/151‑1 (MQ=255)

                                                                                                               gCGACCAGCCTAAACGTTTCACCCC‑GGTCGCGTCTTCCCTCCCCTCAGGCCTGGGCGCGCGGGCCGACCAGGCTCAGCTCGGCGACCTTCTCGCGCCGCTGCGCCAGGTAGCGGGTGCAACTGACACGCAGGAAGGAGGCGAAGTTGAGCa                    <  2:3462881/151‑1 (MQ=255)

                                                                                                                cGACCAGCCTAAACGTTTCACCCC‑GGTCGCGTCTTCCCTCCCCTCAGGCCTGGGCGCGGTGGCCGACCAGGCTCAGCTCGGCGACCGTCTCGCGCCGCTGCGCCAGGTAGCGGGTGCAACTGACACGCCGGACGGAGGCGACGTTGCGCAc                   >  2:7239487/1‑151 (MQ=255)

                                                                                                                 gACCAGCCTAAACGTTTCACCCC‑GGTCGCGTCTTCCCTCCCCTCAGGCCTGGGCGCGCTGGCCGACCAGGCTCAGCTCGGCGACCTTCTCGCGCCGCTGCGCCAGGTAGCGGGTGCAACTGACACGCAGGAAGGAGGCGAAGTTGAGCAcc                  <  1:1514474/151‑1 (MQ=255)

                                                                                                                  aCCAGCCTAAACGTTTCACCCC‑GGTCGCGTCTTCCCTCCCCTCAGGCCTGGGCGCGCTGGCCGACCAGGCTCAGCTCGGCGACCTTCTCGCGCCGCTGCGCCAGGTAGCGGGTGCAACTGACACGCAGGAAGGAGGCGAAGTTGAGCACCt                 <  1:6137480/151‑1 (MQ=255)

                                                                                                                  aCCAGCCTAAACGTTTCACCCC‑GGTCGCGTCTTCCCTCCCCTCAGGCCTGGGCGCGCTGGCCGACCAGGCTCAGCTCGGCGACCTTCTCGCGCCGCTGCGCCAGGTAGCGGGTGCAACTGACACGCAGGAAGGAGGCGAAGTTGAGCACCt                 <  1:2352735/151‑1 (MQ=255)

                                                                                                                  aCCAGCCTAAACGTTTCACCCC‑GGTCGCGTCTTCCCTCCCCTCAGGCCTGGGCGCGCTGGCCGACCAGGCTCAGCGCGGCGACCTTCTCGCGCCGCTGCGCCAGGGAGCGGGTGCAACTGACACGCAGGAAGGAGGCGAAGTTGAGCACCt                 >  2:2348369/1‑151 (MQ=255)

                                                                                                                   ccAGCCTAAACGTTTCACCCC‑GGTCGCGTCTTCCCTCCCCTCAGGCCTGGGCGCGCTGGCCGACCAGGCTCAGCTCGGCGACCTTCTCGCGCCGCTGCGCCAGGTAGCGGGTGCAACTGACACGCAGGAAGGAGGCGAAGTTGAGCACCTc                <  1:9218924/151‑1 (MQ=255)

                                                                                                                   ccAGCCTAAACGATCCACCCC‑GGTCGCGACTTCCCTCCCCTCAGGCCAGGGCGCGCTGGCCGACCAGGCACAGCGCGGCGACCTTCTCGCGCCGCTGCGCCAGGTAGCGGGTGCAACTGCCCCGCAGGAAGGAGGCGAAGTTGAGCAccac                <  1:3525017/151‑3 (MQ=255)

                                                                                                                     aGCCTAAACGTTTCACCCC‑GGTCGCGTCTTCCCTCCCCTCAGGCCTGGGCGCGCTGGCCGACCAGGCTCAGCTCGGCGACCTTCTCGCGCCGCTGCGCCAGGTAGCGGGTGCAACTGACACGCAGGAAGGAGGCGAAGTTGAGCACCTcgc              >  1:4303178/1‑151 (MQ=255)

                                                                                                                     aGCCTAAACGTTTCACCCC‑GGTCGCGTCTTCCCTCCCCTCAGGCCTGGGCGCGCTGGCCGACCAGGCTCAGCTCGGCGACCTTCTCGCGCCGCTGCGCCAGGTAGCGGGTGCAACTGACACGCAGGAAGGAGGCGAAGTTGAGCACCTcgc              >  2:2814848/1‑151 (MQ=255)

                                                                                                                      gCCTAAACGTTTCACCCC‑GGTCGCGTCTTCCCTCCCCTCAGGCCTGGGCGCGCTGGCCGACCAGGCTCAGCTCGGCGACCTTCTCGCGCCGCTGCGCCAGGGAGCGGGTGCAACTGCCACGCAGGACGGAGGCGAAGTTGAGCGCCTcgcc             >  2:1889187/1‑151 (MQ=255)

                                                                                                                       ccTAAACGTTTCACCCC‑GGTCGCGTCTTCCCTCCCCTCAGGGCTGGGCGCGCTGGGCGACCCGGCTCAGCTCGGCGACCTTCTCGCGCCGGTGCGCCCGGTAGCGGGTGCAACTGACACGCCGGAAGGTGGCGAAGTTGAGCACCTcgccg            >  1:7633514/1‑151 (MQ=255)

                                                                                                                            aCGTTTCACCCC‑GGTCGCGTCCTCCCTCCCCTCAGGCCTGGGCGCGCTGGCCGACCAGGCTCAGCTCGGCGACCTTCTCGCGCCGCTGCGCCAGGTAGCGGGTGCAACTGACACGCAGGAAGGAGGCGAAGTTGAGCACCTCGCCGCGATa       >  1:6639350/1‑151 (MQ=255)

                                                                                                                             cGTTTCACCCC‑GGTCGCGTCTTCCCTCCCCTCAGGCCTGGGCGCGCTGGCCGACCAGGCTCAGCGCGGCGACCTTCTCGCGCCGCTGCGCCAGGTAGCGGGTGCAACTGACACGCAGGACGGAGGCGAAGTTGAGCACCTCGCCGCGATAg      >  1:5017595/1‑151 (MQ=255)

                                                                                                                               tttCACCCC‑GGTCGCGTCTTCCCTCCCCTCAGGCCTGGGCGCGCTGGCCGACCAGGCTCAGCTCGGCGACCTTCTCGCGCCGCTGCGCCAGGTAGCGGGTGCAACTGACACGCAGGAAGGAGGCGAAGTTGAGCACCTCGCCGCGATAGTc    <  2:2119900/151‑1 (MQ=255)

||

GCGTCGGCCGGCACCGCCACGCTGTGGCTGACCGCCGAGGGACAGAACGGCGCGGTGGCCCGTGGTGGGCAGGGAGTATTTCACGAGGCGACGCCGGATCGGGGAGGAAGTGCGACCAGCCTAAACGTTTCACCCCGGGTGGGTTCTTCCCTCCCCTCAGGCCTGGGCGCGCTGGCCGACCAGGCTCAGCTCGGCGACCTTCTCGCGCCGCTGCGCCAGGTAGCGGGTGCAACTGACACGCAGGAAGGAGGCGAAGTTGAGCACCTCGCCGCGATAGTCCA  >  NC_008463/5028059‑5028339

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A