breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation annotation gene description
RA 41,753 T→C S207S (AGT→AGC tpsB2 → two‑partner secretion system transporter TpsB2
RA 41,756 G→A R208R (CGG→CGA tpsB2 → two‑partner secretion system transporter TpsB2
RA 72,440 T→C K132E (AAG→GAG)  PA14_RS00315 ← hypothetical protein
RA 96,307 A→C pseudogene (327/723 nt) tagH ← type VI secretion system‑associated FHA domain protein TagH
RA 96,350 T→C pseudogene (284/723 nt) tagH ← type VI secretion system‑associated FHA domain protein TagH
RA 96,353 T→G pseudogene (281/723 nt) tagH ← type VI secretion system‑associated FHA domain protein TagH
RA 96,356 A→C pseudogene (278/723 nt) tagH ← type VI secretion system‑associated FHA domain protein TagH
RA 96,360 T→C pseudogene (274/723 nt) tagH ← type VI secretion system‑associated FHA domain protein TagH
RA 96,391 A→G pseudogene (243/723 nt) tagH ← type VI secretion system‑associated FHA domain protein TagH
RA 96,393 A→C pseudogene (241/723 nt) tagH ← type VI secretion system‑associated FHA domain protein TagH
RA 96,401 G→C pseudogene (233/723 nt) tagH ← type VI secretion system‑associated FHA domain protein TagH
RA 96,406 T→G pseudogene (228/723 nt) tagH ← type VI secretion system‑associated FHA domain protein TagH
RA 96,408 G→C pseudogene (226/723 nt) tagH ← type VI secretion system‑associated FHA domain protein TagH
RA 96,452 +A pseudogene (182/723 nt) tagH ← type VI secretion system‑associated FHA domain protein TagH
RA 151,966 A→G intergenic (‑743/‑415) guaD ← / → PA14_RS00690 guanine deaminase/ABC transporter ATP‑binding protein
RA 155,993 Δ1 bp coding (922/981 nt) PA14_RS00700 → ABC transporter permease
RA 208,430 T→G intergenic (‑36/+52) atsA ← / ← PA14_RS00940 arylsulfatase AtsA/ABC transporter ATP‑binding protein
RA 208,433 G→C intergenic (‑39/+49) atsA ← / ← PA14_RS00940 arylsulfatase AtsA/ABC transporter ATP‑binding protein
RA 480,880 T→C A204A (GCA→GCG PA14_RS02220 ← 16S rRNA (uracil(1498)‑N(3))‑methyltransferase
RA 480,915 T→C K193E (AAA→GAA)  PA14_RS02220 ← 16S rRNA (uracil(1498)‑N(3))‑methyltransferase
RA 559,051 +T intergenic (+619/+70) PA14_RS02545 → / ← rarD glycine cleavage system protein R/EamA family transporter RarD
RA 732,660 G→A intergenic (+114/‑426) tyrS → / → PA14_RS03450 tyrosine‑‑tRNA ligase/16S ribosomal RNA
RA 791,848 C→G P610A (CCC→GCC)  pchF → pyochelin non‑ribosomal peptide synthetase PchF
RA 791,862 3 bp→GCT coding (1842‑1844/5430 nt) pchF → pyochelin non‑ribosomal peptide synthetase PchF
RA 791,890 A→G N624D (AAC→GAC)  pchF → pyochelin non‑ribosomal peptide synthetase PchF
RA 888,038 2 bp→TG coding (1562‑1563/1878 nt) PA14_RS04165 → sigma‑54‑dependent Fis family transcriptional regulator
RA 888,497 A→C intergenic (+143/‑62) PA14_RS04165 → / → PA14_RS04170 sigma‑54‑dependent Fis family transcriptional regulator/LysE family translocator
RA 927,917 T→C I56T (ATC→ACC)  PA14_RS04340 → PAS domain S‑box protein
RA 945,885 Δ1 bp intergenic (‑252/‑69) PA14_RS04400 ← / → PA14_RS04405 2,4'‑dihydroxyacetophenone dioxygenase family protein/helix‑turn‑helix domain‑containing protein
RA 945,897 (C)5→4 intergenic (‑264/‑57) PA14_RS04400 ← / → PA14_RS04405 2,4'‑dihydroxyacetophenone dioxygenase family protein/helix‑turn‑helix domain‑containing protein
RA 966,217 T→C intergenic (+77/‑568) PA14_RS04485 → / → PA14_RS04490 fimbrial protein/response regulator transcription factor
RA 982,940 A→G F293F (TTT→TTC PA14_RS04570 ← ABC transporter permease
JC 1,026,758 (CCA)4→3 coding (353‑355/1356 nt) mpl → UDP‑N‑acetylmuramate:L‑alanyl‑gamma‑D‑glutamyl‑ meso‑diaminopimelate ligase
RA 1,082,923 Δ1 bp pseudogene (230/2518 nt) hrpB → ATP‑dependent helicase HrpB
RA 1,082,944 G→C pseudogene (251/2518 nt) hrpB → ATP‑dependent helicase HrpB
RA 1,082,958 A→G pseudogene (265/2518 nt) hrpB → ATP‑dependent helicase HrpB
RA 1,128,156 3 bp→GCG intergenic (+17/‑42) PA14_RS05280 → / → PA14_RS05285 DUF1302 domain‑containing protein/DUF1329 domain‑containing protein
RA 1,128,160 N→A intergenic (+21/‑40) PA14_RS05280 → / → PA14_RS05285 DUF1302 domain‑containing protein/DUF1329 domain‑containing protein
RA 1,128,162 N→C intergenic (+23/‑38) PA14_RS05280 → / → PA14_RS05285 DUF1302 domain‑containing protein/DUF1329 domain‑containing protein
RA 1,128,165 N→G intergenic (+26/‑35) PA14_RS05280 → / → PA14_RS05285 DUF1302 domain‑containing protein/DUF1329 domain‑containing protein
RA 1,144,646 C→G intergenic (+239/‑13) PA14_RS05355 → / → PA14_RS05360 phosphatidic acid‑binding protein/alkaline phosphatase
RA 1,239,152 N→C intergenic (+150/‑43) PA14_RS05795 → / → lptF alpha/beta hydrolase/LPS export ABC transporter permease LptF
RA 1,239,163 (C)5→4 intergenic (+161/‑32) PA14_RS05795 → / → lptF alpha/beta hydrolase/LPS export ABC transporter permease LptF
RA 1,271,763 Δ1 bp intergenic (+20/+69) PA14_RS05965 → / ← PA14_RS05970 YidB family protein/aldo/keto reductase
RA 1,280,717 (C)6→5 intergenic (‑46/+41) PA14_RS06005 ← / ← PA14_RS06010 DUF4345 domain‑containing protein/M23 family metallopeptidase
RA 1,356,548 G→C R69G (CGT→GGT)  PA14_RS06375 ← MFS transporter
RA 1,375,947 A→G intergenic (+18/‑38) PA14_RS06470 → / → PA14_RS06475 PspA/IM30 family protein/DUF1449 family protein
RA 1,441,164 T→C I804I (ATT→ATC mexK → multidrug efflux RND transporter permease subunit MexK
RA 1,441,861 (C)6→4 intergenic (+31/+11) mexK → / ← PA14_RS06750 multidrug efflux RND transporter permease subunit MexK/DUF4197 domain‑containing protein
RA 1,558,205 A→G Q390Q (CAA→CAG PA14_RS07290 → propionyl‑CoA synthetase
RA 1,647,551 Δ1 bp intergenic (+11/‑91) apbC → / → dcd iron‑sulfur cluster carrier protein ApbC/dCTP deaminase
RA 1,880,872 C→G A502G (GCC→GGC)  PA14_RS08740 → DEAD/DEAH box helicase
RA 2,001,809 T→G D65E (GAT→GAG pgl → 6‑phosphogluconolactonase
RA 2,001,811 A→C Q66P (CAG→CCG)  pgl → 6‑phosphogluconolactonase
RA 2,001,813 G→C V67L (GTG→CTG)  pgl → 6‑phosphogluconolactonase
RA 2,149,425 T→C Q549Q (CAA→CAG PA14_RS09965 ← M14 family metallopeptidase
RA 2,155,075 Δ1 bp intergenic (‑123/‑339) rmf ← / → rlmKL ribosome modulation factor/bifunctional 23S rRNA (guanine(2069)‑N(7))‑methyltransferase RlmK/23S rRNA (guanine(2445)‑N(2))‑methyltransferase RlmL
RA 2,156,071 C→G pseudogene (658/2179 nt) rlmKL → bifunctional 23S rRNA (guanine(2069)‑N(7))‑methyltransferase RlmK/23S rRNA (guanine(2445)‑N(2))‑methyltransferase RlmL
RA 2,156,146 C→A pseudogene (733/2179 nt) rlmKL → bifunctional 23S rRNA (guanine(2069)‑N(7))‑methyltransferase RlmK/23S rRNA (guanine(2445)‑N(2))‑methyltransferase RlmL
RA 2,156,197 Δ1 bp pseudogene (784/2179 nt) rlmKL → bifunctional 23S rRNA (guanine(2069)‑N(7))‑methyltransferase RlmK/23S rRNA (guanine(2445)‑N(2))‑methyltransferase RlmL
RA 2,263,699 Δ1 bp intergenic (‑449/‑100) PA14_RS10520 ← / → cobW CbtB‑domain containing protein/cobalamin biosynthesis protein CobW
RA 2,318,606 A→G E190E (GAA→GAG sbrI → ECF family RNA polymerase sigma factor SbrI
RA 2,341,570 Δ1 bp pseudogene (530/957 nt) PA14_RS10890 → DUF58 domain‑containing protein
RA 2,341,611 Δ1 bp pseudogene (571/957 nt) PA14_RS10890 → DUF58 domain‑containing protein
RA 2,341,704 Δ1 bp pseudogene (664/957 nt) PA14_RS10890 → DUF58 domain‑containing protein
RA 2,354,149 A→G intergenic (‑34/+17) PA14_RS10945 ← / ← lipA lipase secretion chaperone/triacylglycerol lipase
RA 2,354,163 (G)5→4 intergenic (‑48/+3) PA14_RS10945 ← / ← lipA lipase secretion chaperone/triacylglycerol lipase
RA 2,362,330 A→C pseudogene (139/252 nt) oprI ← outer membrane lipoprotei OprI
RA 2,362,363 G→C pseudogene (106/252 nt) oprI ← outer membrane lipoprotei OprI
RA 2,407,435 C→G T115T (ACC→ACG PA14_RS11255 → glutathione S‑transferase
RA 2,407,463 A→G K125E (AAG→GAG)  PA14_RS11255 → glutathione S‑transferase
RA 2,465,074 (G)5→4 intergenic (+73/+253) PA14_RS11645 → / ← PA14_RS11650 DUF3509 domain‑containing protein/hypothetical protein
RA 2,545,609 T→C I509I (ATT→ATC tssI → type VI secretion system tip protein TssI/VgrG
RA 2,545,615 T→C V511V (GTT→GTC tssI → type VI secretion system tip protein TssI/VgrG
RA 2,545,633 2 bp→CT coding (1551‑1552/2070 nt) tssI → type VI secretion system tip protein TssI/VgrG
RA 2,545,663 T→C I527I (ATT→ATC tssI → type VI secretion system tip protein TssI/VgrG
RA 2,589,402 C→T intergenic (‑45/+1) nuoK ← / ← nuoJ NADH‑quinone oxidoreductase subunit NuoK/NADH‑quinone oxidoreductase subunit J
RA 2,594,570 (G)6→5 coding (1096/1350 nt) nuoF ← NADH‑quinone oxidoreductase subunit NuoF
RA 2,594,625 Δ1 bp coding (1041/1350 nt) nuoF ← NADH‑quinone oxidoreductase subunit NuoF
RA 2,594,659 Δ1 bp coding (1007/1350 nt) nuoF ← NADH‑quinone oxidoreductase subunit NuoF
RA 2,651,339 T→C F68L (TTC→CTC)  PA14_RS12480 → MFS transporter
RA 2,651,357 A→G N74D (AAC→GAC)  PA14_RS12480 → MFS transporter
RA 2,707,727 N→G ?75P (CNT→CCT)  PA14_RS12740 ← DUF2285 domain‑containing protein
RA 2,730,678 C→T intergenic (+16/+99) PA14_RS12860 → / ← ctpH anti‑virulence regulator CigR family protein/methyl‑accepting chemotaxis protein CtpH
RA 2,730,681 2 bp→CG intergenic (+19/+95) PA14_RS12860 → / ← ctpH anti‑virulence regulator CigR family protein/methyl‑accepting chemotaxis protein CtpH
RA 2,762,006 A→G K119E (AAG→GAG)  PA14_RS12995 → lysophospholipid acyltransferase family protein
RA 2,762,024 A→G N125D (AAC→GAC)  PA14_RS12995 → lysophospholipid acyltransferase family protein
RA 2,762,027 A→G N126D (AAC→GAC)  PA14_RS12995 → lysophospholipid acyltransferase family protein
RA 2,762,030 A→G K127E (AAG→GAG)  PA14_RS12995 → lysophospholipid acyltransferase family protein
RA 2,787,777 C→G pseudogene (855/3090 nt) PA14_RS13085 → CusA/CzcA family heavy metal efflux RND transporter
RA 2,787,784 T→G pseudogene (862/3090 nt) PA14_RS13085 → CusA/CzcA family heavy metal efflux RND transporter
RA 2,787,820 +A pseudogene (898/3090 nt) PA14_RS13085 → CusA/CzcA family heavy metal efflux RND transporter
RA 2,787,829 2 bp→AG pseudogene (907‑908/3090 nt) PA14_RS13085 → CusA/CzcA family heavy metal efflux RND transporter
RA 2,787,861 3 bp→GTG pseudogene (939‑941/3090 nt) PA14_RS13085 → CusA/CzcA family heavy metal efflux RND transporter
RA 2,787,867 T→A pseudogene (945/3090 nt) PA14_RS13085 → CusA/CzcA family heavy metal efflux RND transporter
RA 2,787,871 Δ1 bp pseudogene (949/3090 nt) PA14_RS13085 → CusA/CzcA family heavy metal efflux RND transporter
RA 2,787,873 A→C pseudogene (951/3090 nt) PA14_RS13085 → CusA/CzcA family heavy metal efflux RND transporter
RA 2,787,877 C→G pseudogene (955/3090 nt) PA14_RS13085 → CusA/CzcA family heavy metal efflux RND transporter
RA 2,787,882 +G pseudogene (960/3090 nt) PA14_RS13085 → CusA/CzcA family heavy metal efflux RND transporter
RA 2,787,895 Δ1 bp pseudogene (973/3090 nt) PA14_RS13085 → CusA/CzcA family heavy metal efflux RND transporter
RA 2,807,266 G→C V137L (GTG→CTG)  PA14_RS13175 → leucine‑rich repeat‑containing protein kinase family protein
RA 2,840,442 T→C intergenic (+42/‑47) PA14_RS13335 → / → PA14_RS13340 LysR substrate‑binding domain‑containing protein/sigma‑70 family RNA polymerase sigma factor
RA 2,840,444 T→C intergenic (+44/‑45) PA14_RS13335 → / → PA14_RS13340 LysR substrate‑binding domain‑containing protein/sigma‑70 family RNA polymerase sigma factor
RA 2,885,933 G→C G71G (GGG→GGC gcvH → glycine cleavage system protein GcvH
RA 2,955,357 A→G L314L (TTG→CTG)  PA14_RS13700 ← PepSY domain‑containing protein
RA 2,955,433 A→G A288A (GCT→GCC PA14_RS13700 ← PepSY domain‑containing protein
RA 2,955,468 A→G L277L (TTG→CTG)  PA14_RS13700 ← PepSY domain‑containing protein
RA 2,985,345 A→G K2233E (AAA→GAA)  pvdD → pyoverdine non‑ribosomal peptide synthetase PvdD
RA 3,051,401 T→C N373D (AAC→GAC)  PA14_RS13990 ← SfnB family sulfur acquisition oxidoreductase
RA 3,051,413 T→C K369E (AAG→GAG)  PA14_RS13990 ← SfnB family sulfur acquisition oxidoreductase
RA 3,051,443 T→C K359E (AAG→GAG)  PA14_RS13990 ← SfnB family sulfur acquisition oxidoreductase
RA 3,114,336 A→G intergenic (+69/‑63) PA14_RS14235 → / → PA14_RS14240 glucose/quinate/shikimate family membrane‑bound PQQ‑dependent dehydrogenase/TonB‑dependent receptor
RA 3,150,987 Δ1 bp intergenic (‑192/+24) pvcA ← / ← PA14_RS14420 paerucumarin biosynthesis protein PvcA/asparaginase
RA 3,198,146 Δ1 bp coding (230/1980 nt) PA14_RS14610 ← cation/acetate symporter
RA 3,198,441 T→G A547A (GCA→GCC PA14_RS14615 ← acyl‑CoA synthetase
RA 3,281,477 G→C intergenic (‑25/+44) katE ← / ← PA14_RS14985 catalase HPII/general stress protein
RA 3,356,495 G→C intergenic (+22/+49) PA14_RS15330 → / ← PA14_RS31420 hypothetical protein/hypothetical protein
RA 3,373,667 G→C V352V (GTC→GTG PA14_RS15385 ← aminotransferase class V‑fold PLP‑dependent enzyme
RA 3,374,601 A→G F41S (TTC→TCC)  PA14_RS15385 ← aminotransferase class V‑fold PLP‑dependent enzyme
RA 3,387,854 A→C I38L (ATC→CTC)  cynS → cyanase
RA 3,387,865 A→C T41T (ACA→ACC cynS → cyanase
RA 3,387,868 A→C L42F (TTA→TTC cynS → cyanase
RA 3,387,881 A→C M47L (ATG→CTG)  cynS → cyanase
RA 3,387,884 T→C F48L (TTC→CTC)  cynS → cyanase
RA 3,387,941 A→C M67L (ATG→CTG)  cynS → cyanase
RA 3,390,474 Δ1 bp pseudogene (440/1997 nt) PA14_RS15455 → alkaline phosphatase D family protein
RA 3,390,485 Δ1 bp pseudogene (451/1997 nt) PA14_RS15455 → alkaline phosphatase D family protein
RA 3,390,498 A→C pseudogene (464/1997 nt) PA14_RS15455 → alkaline phosphatase D family protein
RA 3,423,281 A→G K130K (AAA→AAG mexY → multidrug efflux RND transporter permease subunit MexY
RA 3,423,414 C→G Q175E (CAG→GAG)  mexY → multidrug efflux RND transporter permease subunit MexY
RA 3,424,152 C→G Q421E (CAA→GAG)  mexY → multidrug efflux RND transporter permease subunit MexY
RA 3,424,154 A→G Q421E (CAA→GAG mexY → multidrug efflux RND transporter permease subunit MexY
RA 3,424,176 A→G T429A (ACC→GCC)  mexY → multidrug efflux RND transporter permease subunit MexY
RA 3,424,199 A→G Q436Q (CAA→CAG mexY → multidrug efflux RND transporter permease subunit MexY
RA 3,426,133 Δ1 bp intergenic (+104/+138) mexY → / ← PA14_RS15615 multidrug efflux RND transporter permease subunit MexY/YjiK family protein
RA 3,443,292 C→G P459A (CCC→GCC)  PA14_RS15680 → GntP family permease
RA 3,489,979 (G)5→4 pseudogene (154/718 nt) PA14_RS15905 ← DUF1294 domain‑containing protein
RA 3,515,863 T→C intergenic (+617/+267) PA14_RS16005 → / ← PA14_RS16010 AAA family ATPase/DUF4354 family protein
RA 3,543,662 A→G T494A (ACG→GCG)  PA14_RS16110 → PucR family transcriptional regulator
RA 3,566,665 Δ1 bp pseudogene (1016/1275 nt) PA14_RS16215 → fatty acid desaturase
RA 3,566,716 A→G pseudogene (1067/1275 nt) PA14_RS16215 → fatty acid desaturase
RA 3,566,730 A→G pseudogene (1081/1275 nt) PA14_RS16215 → fatty acid desaturase
RA 3,566,749 A→G pseudogene (1100/1275 nt) PA14_RS16215 → fatty acid desaturase
RA 3,566,751 A→G pseudogene (1102/1275 nt) PA14_RS16215 → fatty acid desaturase
RA 3,566,769 A→G pseudogene (1120/1275 nt) PA14_RS16215 → fatty acid desaturase
RA 3,566,788 A→G pseudogene (1139/1275 nt) PA14_RS16215 → fatty acid desaturase
RA 3,566,823 +A pseudogene (1174/1275 nt) PA14_RS16215 → fatty acid desaturase
RA 3,644,364 Δ1 bp intergenic (‑115/‑120) sohB ← / → PA14_RS16560 protease SohB/histidine phosphatase family protein
RA 3,662,614 T→C F10F (TTT→TTC gloB → hydroxyacylglutathione hydrolase
RA 3,670,384 A→G Q128Q (CAA→CAG PA14_RS16675 → ABC transporter permease
RA 3,670,537 Δ1 bp coding (537/1020 nt) PA14_RS16675 → ABC transporter permease
RA 3,670,543 +A coding (543/1020 nt) PA14_RS16675 → ABC transporter permease
RA 3,673,519 N→C intergenic (+68/+30) fabI → / ← PA14_RS16690 enoyl‑ACP reductase FabI/SurA N‑terminal domain‑containing protein
RA 3,673,524 A→G intergenic (+73/+25) fabI → / ← PA14_RS16690 enoyl‑ACP reductase FabI/SurA N‑terminal domain‑containing protein
RA 3,673,534 3 bp→CTC intergenic (+83/+13) fabI → / ← PA14_RS16690 enoyl‑ACP reductase FabI/SurA N‑terminal domain‑containing protein
RA 3,711,749 T→C L37L (TTG→CTG)  cobA → uroporphyrinogen‑III C‑methyltransferase
RA 3,711,791 G→C V51L (GTG→CTG)  cobA → uroporphyrinogen‑III C‑methyltransferase
RA 3,769,180 C→G A44A (GCG→GCC pscL ← SctL family type III secretion system stator protein PscL
RA 3,879,553 A→G F52L (TTC→CTC)  PA14_RS17695 ← LysE family translocator
RA 3,909,637 (G)5→4 intergenic (‑133/+14) PA14_RS17840 ← / ← brnQ DUF599 domain‑containing protein/branched‑chain amino acid transport system II carrier protein
RA 3,933,352 C→G P346P (CCC→CCG PA14_RS17955 → MFS transporter
RA 4,167,196 Δ1 bp intergenic (+41/‑131) PA14_RS19010 → / → PA14_RS19015 VOC family protein/MFS transporter
RA 4,407,236 A→G intergenic (+107/‑205) PA14_RS20115 → / → toxA ATP‑grasp domain‑containing protein/exotoxin A
RA 4,565,005 C→G G319A (GGG→GCG)  PA14_RS20885 ← anthranilate synthase component 1
RA 4,565,040 A→G F307F (TTT→TTC PA14_RS20885 ← anthranilate synthase component 1
RA 4,565,093 C→G G290R (GGC→CGC)  PA14_RS20885 ← anthranilate synthase component 1
RA 4,659,805 A→G intergenic (+45/+54) alpR → / ← PA14_RS21395 transcriptional regulator AlpR/tRNA‑Arg
RA 4,659,822 A→G intergenic (+62/+37) alpR → / ← PA14_RS21395 transcriptional regulator AlpR/tRNA‑Arg
RA 4,659,831 Δ1 bp intergenic (+71/+28) alpR → / ← PA14_RS21395 transcriptional regulator AlpR/tRNA‑Arg
RA 4,700,807 Δ1 bp intergenic (+165/‑150) PA14_RS21585 → / → pbpG aspartate/tyrosine/aromatic aminotransferase/D‑alanyl‑D‑alanine endopeptidase
RA 4,707,658 G→C A165A (GCG→GCC PA14_RS21625 → zinc‑binding alcohol dehydrogenase family protein
RA 4,707,787 G→C A208A (GCG→GCC PA14_RS21625 → zinc‑binding alcohol dehydrogenase family protein
RA 4,760,743 A→G L73P (CTT→CCT)  PA14_RS21850 ← HNH endonuclease
RA 4,819,717 (C)5→4 intergenic (‑115/+55) PA14_RS22115 ← / ← lepB DUF4845 domain‑containing protein/signal peptidase I
RA 4,947,626 A→G K147R (AAG→AGG)  PA14_RS22670 → DNA polymerase Y family protein
RA 4,947,631 A→C M149L (ATG→CTG)  PA14_RS22670 → DNA polymerase Y family protein
RA 4,947,633 +G coding (447/1416 nt) PA14_RS22670 → DNA polymerase Y family protein
RA 4,947,636 C→A R150R (CGC→CGA PA14_RS22670 → DNA polymerase Y family protein
RA 4,947,639 Δ1 bp coding (453/1416 nt) PA14_RS22670 → DNA polymerase Y family protein
RA 4,947,641 2 bp→GC coding (455‑456/1416 nt) PA14_RS22670 → DNA polymerase Y family protein
RA 4,947,645 G→C Q153H (CAG→CAC PA14_RS22670 → DNA polymerase Y family protein
RA 4,947,649 2 bp→GG coding (463‑464/1416 nt) PA14_RS22670 → DNA polymerase Y family protein
RA 4,947,652 A→C I156M (ATT→CTG)  PA14_RS22670 → DNA polymerase Y family protein
RA 4,947,654 2 bp→GG coding (468‑469/1416 nt) PA14_RS22670 → DNA polymerase Y family protein
RA 4,947,683 A→G H166R (CAC→CGC)  PA14_RS22670 → DNA polymerase Y family protein
RA 4,957,532 A→G intergenic (‑354/‑318) PA14_RS22700 ← / → PA14_RS22705 16S ribosomal RNA/exonuclease SbcCD subunit D C‑terminal domain‑containing protein
RA 4,957,549 2 bp→AT intergenic (‑371/‑300) PA14_RS22700 ← / → PA14_RS22705 16S ribosomal RNA/exonuclease SbcCD subunit D C‑terminal domain‑containing protein
RA 4,978,469 Δ1 bp intergenic (+125/‑20) PA14_RS31455 → / → PA14_RS22760 hypothetical protein/inorganic phosphate transporter
RA 4,997,786 T→C K173E (AAG→GAG)  PA14_RS22840 ← DUF853 family protein
RA 5,028,119 Δ1 bp intergenic (‑41/+96) PA14_RS22975 ← / ← PA14_RS22980 Nramp family divalent metal transporter/ribbon‑helix‑helix domain‑containing protein
RA 5,028,197 Δ1 bp intergenic (‑119/+18) PA14_RS22975 ← / ← PA14_RS22980 Nramp family divalent metal transporter/ribbon‑helix‑helix domain‑containing protein
RA 5,028,199 G→C intergenic (‑121/+16) PA14_RS22975 ← / ← PA14_RS22980 Nramp family divalent metal transporter/ribbon‑helix‑helix domain‑containing protein
RA 5,028,201 2 bp→CG intergenic (‑123/+13) PA14_RS22975 ← / ← PA14_RS22980 Nramp family divalent metal transporter/ribbon‑helix‑helix domain‑containing protein
RA 5,103,224 2 bp→CA coding (818‑819/1185 nt) ftsZ ← cell division protein FtsZ
RA 5,103,259 T→G M262L (ATG→CTG)  ftsZ ← cell division protein FtsZ
RA 5,103,291 C→T G251E (GGA→GAA)  ftsZ ← cell division protein FtsZ
RA 5,103,303 G→T T247N (ACC→AAC)  ftsZ ← cell division protein FtsZ
RA 5,103,322 G→T P241T (CCC→ACC)  ftsZ ← cell division protein FtsZ
RA 5,118,016 C→T G59D (GGC→GAC)  ftsL ← cell division protein FtsL
MC JC 5,131,729 Δ34 bp pseudogene (288‑321/1180 nt) PA14_RS23490 ← acyl‑CoA dehydrogenase family protein
RA 5,198,405 T→C intergenic (+31/+37) PA14_RS23800 → / ← mdpA ABC transporter substrate‑binding protein/metallopeptidase MdpA
RA 5,256,362 N→C ?82A (GNC→GCC)  dnaB → replicative DNA helicase
RA 5,256,376 T→C F87L (TTC→CTC)  dnaB → replicative DNA helicase
RA 5,256,379 C→G P88A (CCC→GCC)  dnaB → replicative DNA helicase
RA 5,256,382 A→G N89D (AAC→GAC)  dnaB → replicative DNA helicase
RA 5,315,218 Δ1 bp coding (325/330 nt) PA14_RS31685 → FimD/PapC N‑terminal domain‑containing protein
RA 5,358,513 Δ1 bp pseudogene (774/1285 nt) PA14_RS24540 → site‑specific integrase
RA 5,395,470 Δ1 bp intergenic (‑103/+50) PA14_RS24730 ← / ← PA14_RS24735 hypothetical protein/ATP‑binding protein
RA 5,395,476 (G)5→4 intergenic (‑109/+44) PA14_RS24730 ← / ← PA14_RS24735 hypothetical protein/ATP‑binding protein
RA 5,514,060 (G)5→4 intergenic (+15/+136) PA14_RS25260 → / ← PA14_RS25265 tRNA‑Met/HigA family addiction module antitoxin
RA 5,530,315 A→G K229K (AAA→AAG PA14_RS25330 → iron ABC transporter substrate‑binding protein
RA 5,648,548 A→G intergenic (‑223/‑38) PA14_RS25880 ← / → PA14_RS25885 AraC family transcriptional regulator/MaoC/PaaZ C‑terminal domain‑containing protein
RA 5,648,573 A→G intergenic (‑248/‑13) PA14_RS25880 ← / → PA14_RS25885 AraC family transcriptional regulator/MaoC/PaaZ C‑terminal domain‑containing protein
RA 5,757,525 G→C Q27E (CAG→GAG)  PA14_RS26415 ← ferredoxin reductase
RA 5,757,527 G→C P26R (CCC→CGC)  PA14_RS26415 ← ferredoxin reductase
RA 5,791,938 A→G intergenic (+32/‑103) nadE → / → choE ammonia‑dependent NAD(+) synthetase/cholinesterase ChoE
RA 5,791,973 Δ1 bp intergenic (+67/‑68) nadE → / → choE ammonia‑dependent NAD(+) synthetase/cholinesterase ChoE
RA 5,792,010 A→G intergenic (+104/‑31) nadE → / → choE ammonia‑dependent NAD(+) synthetase/cholinesterase ChoE
JC 5,809,296 Δ3 bp intergenic (‑182/+35) rpsF ← / ← rlmB 30S ribosomal protein S6/23S rRNA (guanosine(2251)‑2'‑O)‑methyltransferase RlmB
RA 5,809,308 Δ1 bp intergenic (‑194/+25) rpsF ← / ← rlmB 30S ribosomal protein S6/23S rRNA (guanosine(2251)‑2'‑O)‑methyltransferase RlmB
RA 5,809,309 C→G intergenic (‑195/+24) rpsF ← / ← rlmB 30S ribosomal protein S6/23S rRNA (guanosine(2251)‑2'‑O)‑methyltransferase RlmB
RA 5,809,311 C→G intergenic (‑197/+22) rpsF ← / ← rlmB 30S ribosomal protein S6/23S rRNA (guanosine(2251)‑2'‑O)‑methyltransferase RlmB
RA 5,809,315 G→C intergenic (‑201/+18) rpsF ← / ← rlmB 30S ribosomal protein S6/23S rRNA (guanosine(2251)‑2'‑O)‑methyltransferase RlmB
RA 5,809,318 G→C intergenic (‑204/+15) rpsF ← / ← rlmB 30S ribosomal protein S6/23S rRNA (guanosine(2251)‑2'‑O)‑methyltransferase RlmB
RA 5,809,365 T→C K239E (AAG→GAG)  rlmB ← 23S rRNA (guanosine(2251)‑2'‑O)‑methyltransferase RlmB
RA 5,866,730 T→G A733A (GCA→GCC PA14_RS26890 ← ATP‑binding protein
RA 5,905,079 A→G F731F (TTT→TTC glnE ← bifunctional [glutamate‑‑ammonia ligase]‑adenylyl‑L‑tyrosine phosphorylase/[glutamate‑‑ammonia‑ligase] adenylyltransferase
RA 5,931,597 Δ1 bp intergenic (‑16/‑162) PA14_RS27130 ← / → PA14_RS27135 LysR family transcriptional regulator/MFS transporter
RA 5,931,608 (T)6→5 intergenic (‑27/‑151) PA14_RS27130 ← / → PA14_RS27135 LysR family transcriptional regulator/MFS transporter
RA 5,968,025 C→G P426A (CCC→GCC)  phaC → class II poly(R)‑hydroxyalkanoic acid synthase
RA 6,070,064 Δ1 bp pseudogene (533/1086 nt) PA14_RS27735 → oxidoreductase
RA 6,070,078 T→C pseudogene (547/1086 nt) PA14_RS27735 → oxidoreductase
RA 6,070,084 T→C pseudogene (553/1086 nt) PA14_RS27735 → oxidoreductase
RA 6,070,093 Δ1 bp pseudogene (562/1086 nt) PA14_RS27735 → oxidoreductase
RA 6,070,098 G→C pseudogene (567/1086 nt) PA14_RS27735 → oxidoreductase
RA 6,070,100 T→C pseudogene (569/1086 nt) PA14_RS27735 → oxidoreductase
RA 6,070,106 Δ1 bp pseudogene (575/1086 nt) PA14_RS27735 → oxidoreductase
RA 6,070,122 T→C pseudogene (591/1086 nt) PA14_RS27735 → oxidoreductase
RA 6,076,066 G→C R105R (CGG→CGC PA14_RS27775 → methyltransferase
RA 6,102,509 (G)5→4 intergenic (+35/+18) yrfG → / ← lysM GMP/IMP nucleotidase/peptidoglycan‑binding protein LysM
RA 6,128,635 Δ1 bp intergenic (+100/‑108) PA14_RS28015 → / → gshA PaaI family thioesterase/glutamate‑‑cysteine ligase
RA 6,205,906 Δ1 bp intergenic (‑11/+186) PA14_RS28345 ← / ← PA14_RS28350 type VI secretion system tip protein VgrG/Hcp family type VI secretion system effector
RA 6,250,873 Δ1 bp intergenic (+16/‑163) dadR → / → PA14_RS28560 transcriptional regulator DadR/FAD‑binding oxidoreductase

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NC_008463 1639794–1640248 1641126–1640395 148–1333 264 [260] [262] 264 tssI type VI secretion system tip protein TssI/VgrG
* * ÷ NC_008463 3555798–3558066 3559468–3558092 27–3671 265 [262] [258] 264 [phzG]–[phzD] [phzG],phzF,phzE,[phzD]
* * ÷ NC_008463 4952227–4952922 4953910–4952951 30–1684 265 [262] [261] 271 [PA14_RS22685] [PA14_RS22685]

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_008463 3175697 =367 (0.720)492 (1.060) 206/274 0.2 63.0% intergenic (+390/+106) PA14_RS30505/PA14_RS14515 hypothetical protein/hypothetical protein
?NC_008463 = 3177289 242 (0.520)intergenic (‑69/‑218) PA14_RS14525/PA14_RS14530 hypothetical protein/Tn3 family transposase