Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A4 F1 I1 R1 260 537.2 23268138 99.2% 23081992 151.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_008463 5,028,197 Δ1 bp intergenic (‑119/+18) PA14_RS22975 ← / ← PA14_RS22980 Nramp family divalent metal transporter/ribbon‑helix‑helix domain‑containing protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0084635,028,1950G.99.6% 2446.7 / 2.3 495intergenic (‑117/+20)PA14_RS22975/PA14_RS22980Nramp family divalent metal transporter/ribbon‑helix‑helix domain‑containing protein
Reads supporting (aligned to +/- strand):  ref base G (1/1);  new base . (250/243);  total (251/244)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.86e-01

Only 100 of 533 total aligned reads displayed.

TTGCGCAGCGGCGAGGCGTCGGCCGGCACCGCCACGCTGTGGCTGACCGCCGAGGGACAGAACGGCGCGGTGGCCCGTGGTGGGCAGGGAGTATTTCACGAGGCGACGCCGGATCGGGGAGGAAGTGCGACCAGCCTAAACGTTTCACCCCGGGTGGGTTCTTCCCTCCCCTCAGGCCTGGGCGCGCTGGCCGACCAGGCTCAGCTCGGCGACCTTCTCGCGCCGCTGCGCCAGGTAGCGGGTGCAACTGACACGCAGGAAGGAGGCGAAGTTGAGCACCTCGCCGCGATAGTCCA  >  NC_008463/5028044‑5028339
                                                                                                                                                       |                                                                                                                                                
ttGCGCAGCGGCGAGGCGTCGGCCGGCACCGCCACGCTGTGGCTGACCGCCGAGGGACAGAACGGCGCGGTGG‑CCGTGGTGGGCAGGGAGTATTTCACGAGGCGACGCCGGATCGGGGAGGAAGTGCGACCAGCCTAAACGTTTCACCCCg                                                                                                                                                  <  1:1970103/151‑1 (MQ=255)
ttGCGCAGCGGCGAGGCGTCGGCCGGCACCGCCACGCTGTGGCTGACCGCCGAGGGACAGAACGGCGCGGTGG‑CCGTGGTGGGCAGGGAGTATTTCACGAGGCGACGCCGGATCGGGGAGGAAGTGCGACCAGCCTAAACGTTTCACCCCg                                                                                                                                                  >  1:7428410/1‑151 (MQ=255)
  gcgcAGCGGCGAGGCGTCGGCCGGCACCGCCACGCTGTGGCTGACCGCCGAGGGACAGAACGGCGCGGTGG‑CCGTGGTGGGCAGGGAGTATTTCACGAGGCGACGCCGGATCGGGGAGGAAGTGCGACCAGCCTAAACGTTTCACCCCggt                                                                                                                                                <  1:8358048/151‑2 (MQ=255)
   cgcAGCGGCGAGGCGTCGGCCGGCACCGCCACGCTGTGGCTGACCGCCGAGGGACAGAACGGCGCGGTGG‑CCGTGGTGGGCAGGGAGTATTTCACGAGGCGACGCCGGATCGGGGAGGAAGTGCGACCAGCCTAAACGTTTCACCCCggtc                                                                                                                                               <  2:6296229/151‑3 (MQ=255)
    gcAGCGGCGAGGCGTCGGCCGGCACCGCCACGCTGTGGCTGACCGCCGAGGNACAGAACGGCGCGGTGG‑CCGTGGTGGGCAGGGAGTATTTCACGAGGCGACGCCGGATCGGGGAGGAAGTGCGACCAGCCTAAACGTTTCACCCCggtcg                                                                                                                                              >  1:6092669/1‑148 (MQ=255)
     cAGCGGCGAGGCGTCGGCCGGCACCGCCACGCTGTGGCTGACCGCCGAGGGACAGAACGGCGCGGTGG‑CCGTGGTGGGCAGGGAGTATTTCACGAGGCGACGCCGGATCGGGGAGGAAGTGCGACCAGCCTAAACGTTTCACCCCggtcgc                                                                                                                                             >  1:3142435/1‑147 (MQ=255)
     cAGCGGCGAGGCGTCGGCCGGCACCGCCACGCTGTGGCTGACCGCCGAGGGACAGAACGGCGCGGTGG‑CCGTGGTGGGCAGGGAGTATTTCACGAGGCGACGCCGGATCGGGGAGGAAGTGCGACCAGCCTAAACGTTTCACCCCggtcgc                                                                                                                                             >  1:6975506/1‑147 (MQ=255)
     cAGCGGCGAGGCGTCGGCCGGCACCGCCACGCTGTGGCTGACCGCCGAGGGACAGAACGGCGCGGTGG‑CCGTGGTGGGCAGGGAGTATTTCACGAGGCGACGCCGGATCGGGGAGGAAGTGCGACCAGCCTAAACGTTTCACCCCggtcgc                                                                                                                                             <  1:45529/151‑5 (MQ=255)
      aGCGGCGAGGCGTCGGCCGGCACCGCCACGCTGTGGCTGACCGCCGAGGGACAGAACGGCGCGGTGG‑CCGTGGTGGGCAGGGAGTATTTCACGAGGCGACGCCGGATCGGGGAGGAAGTGCGACCAGCCTAAACGTTTCACCCCggtctcg                                                                                                                                            >  2:1136080/1‑146 (MQ=255)
        cggcgAGGCGTCGGCCGGCACCGCCACGCTGTGGCTGACCGCCGAGGGACAGAACGGCGCGGTGG‑CCGTGGTGGGCAGGGAGTATTTCACGAGGCGACGCCGGATCGGGGAGGAAGTGCGACCAGCCTAAACGTTTCACCCCggtcgcgtc                                                                                                                                          >  2:9857362/1‑144 (MQ=255)
                  tCGGCCGGCACCGCCACGCTGTGGCTGACCGCCGAGGGACAGAACGGCGCGGTGG‑CCGTGGTGGGCAGGGAGTATTTCACGAGGCGACGCCGGATCGGGGAGGAAGTGCGACCAGCCTAAACGTTTCACCCC‑GGTCGCGTCTTccctcccc                                                                                                                               <  2:93450/151‑1 (MQ=255)
                    ggccggcACCGCCACGCTGTGGCTGACCGCCGAGGGACAGACCGGCGCGGTGG‑CCGTGGTGGGCAGGGAGTATTTCACGAGGCGACGCCGGATC‑GGGAGGAAGTGCGACCAGCCTAAACGTTTCACCCC‑GGTCGCGTCTTCCCTCCCCTCa                                                                                                                            <  2:8448773/151‑1 (MQ=255)
                    ggccggcACCGCCACGCTGTGGCTGACCGCCGAGGGACAGAACGGCGCGGTGG‑CCGTGGTGGGCAGGGAGTATTTCACGAGGCGACGCCGGATCGGGGAGGAAGTGCGACCAGCCTAAACGTTTCACCCC‑GGTCGCGTCTTccctcccctc                                                                                                                             >  1:11279071/1‑151 (MQ=255)
                     gccggcACCGCCACGCTGTGGCTGACCGCCGAGGGACAGAACGGCGCGGTGG‑CCGTGGTGGGCAGGGAGTATTTCACGAGGCGACGCCGGATCGGGGAGGAAGTGCGACCAGCCTAAACGTTTCACCCC‑GGTCGCGTCTTCCCTCCCCTCa                                                                                                                            <  2:4463476/151‑1 (MQ=255)
                       cggcACCGCCACGCTGTGGCTGACCGCCGAGGGACAGAACGGCGCGGTGG‑CCGTGGTGGGCAGGGAGTATTTCACGAGGCGACGCCGGATCGGGGAGGAAGTGCGACCAGCCTAAACGTTTCACCCC‑GGTCGCGTCTTCCCTCCCCTCAgg                                                                                                                          <  1:6706510/151‑1 (MQ=255)
                        ggcACCGCCACGCTGTGGCTGACCGCCGAGGGACAGAACGGCGCGGTGG‑CCGTGGTGGGCAGGGAGTATTTCACGAGGCGACGCCGGATCGGGGAGGAAGTGCGACCAGCCTAAACGTTTCACCCC‑GGTCGCGTCTTCCCTCCCCTCAGGc                                                                                                                         <  2:7643310/151‑1 (MQ=255)
                         gcACCGCCACGCTGTGGCTGACCGCCGAGGGACAGAACGGCGCGGTGG‑CCGTGGTGGGCAGGGAGTATTTCACGAGGCGACGCCGGATCGGGGAGGAAGTGCGACCAGCCTAAACGTTTCACCCC‑GGTCGCGTCTTCCCTCCCCTCAGGcc                                                                                                                        <  2:6094854/151‑1 (MQ=255)
                              gCCACGCTGTGGCTGACCGCCGAGGGACAGAACGGCGCGGTGG‑CCGTGGTGGGCAGGGAGTATTTCACGAGGCGACGCCGGATCGGGGAGTAAGTGCGACCAGCCTAAACGTTTCACCCC‑GGTCGCGTCTTCCCTCCCCTCAGGCCTGGgc                                                                                                                   >  2:1496363/1‑151 (MQ=255)
                              gCCACGCTGTGGCTGACCGCCGAGGGACAGAACGGCGCGGTGG‑CCGTGGTGGGCAGGGAGTATTTCACGAGGCGACGCCGGATCGGGGAGGAAGTGCGACCAGCCTAAACGTTTCACCCC‑GGTCGCGTCTTCCCTCCCCTCAGGCCTGGgc                                                                                                                   >  2:1086700/1‑151 (MQ=255)
                                     tgtgGCTGACCGCCGAGGGACAGAACGGCGCGGTGG‑CCGTGGTGGGCAGGGAGTATTTCACGAGGCGACGCCGGATCGGGGAGGAAGTGCGACCAGCCTAAACGTTTCACCCC‑GGTCGCGTCTTCCCTCCCCTCAGGCCTGGGCGCGCTgg                                                                                                            <  1:2228943/151‑1 (MQ=255)
                                      gtgGCTGACCGCCGAGGGACAGAACGGCGCGGTGG‑CCGTGGTGGGCAGGGAGTATTTCACGAGGCGTCGCCGGATCGGGGAGGAAGTGCGACCAGCCTAAACGTTTCACCCC‑GGTCGCGTCTTCCCTCCCCTCAGGCCTGGGCGCGCTGGc                                                                                                           <  1:321670/151‑1 (MQ=255)
                                       tgGCTGACCGCCGAGGGACAGAACGGCGCGGTGG‑CCGTGGTGGGCAGGGAGTATTTCACGAGGCGACGCCGGATCGGGGAGGAAGTGCGACCAGCCTAAACGTTTCACCCC‑GGTCGCGTCTTCCCTCCCCTCAGGCCTGGGCGCGCTGGcc                                                                                                          <  1:3370087/151‑1 (MQ=255)
                                        ggCTGACCGCCGAGGGACAGAACGGCGCGGTGG‑CCGTGGTGGGCAGGGAGTATTTCACGAGGCGACGCCGGATCGGGGAGGAAGTGCGACCAGCCTAAACGTTTCACCCC‑GGTCGCGTCTTCCCTCCCCTCAGGCCTGGGCGCGCTGGCCg                                                                                                         >  2:5115302/1‑151 (MQ=255)
                                          cTGACCGCCGAGGGACAGAACGGCGCGGTGG‑CCGTGGTGGGCAGGGAGTATTTCACGAGGCGACGCCGGATCGGGGAGGAAGTGCGACCAGCCTAAACGTTTCACCCC‑GGTCGCGTCTTCCCTCCCCTCAGGCCTGGGCGCGCTGGCCGAc                                                                                                       <  2:3691340/151‑1 (MQ=255)
                                            gACCGCCGAGGGACAGAACGGCGCGGTGG‑CCGTGGTGGGCAGGGAGTATTTCACGAGGCGACGCCGGATCGGGGAGGAAGTGCGAGCAGCCAGAACGTTTCTCCCC‑GGTCGCGTCTTCCCTCCCCTCAGGCCAGGGCGCGCTGGCCGAcct                                                                                                     >  1:4023009/1‑150 (MQ=255)
                                               cgccgAGGGACAGAACGGCGCGGTGG‑CCGTGGTGGGCAGGGAGTATTTCACGAGGCGAAGCCGGATCGGGGAGGAAGTGCGACCAGCCTAAACGTTTCACCCC‑GGTCGCGTCTTCCCTCCCCTCAGGCCTGGGCGCGCTGGCCGACCAGGc                                                                                                  >  1:4374065/1‑151 (MQ=255)
                                                 ccgAGGGACAGAACGGCGCGGTGG‑CCGTGGTGGGCAGGGAGTATTTCACGAGGCGACGCCGGATCGGGGAGGAAGTGCGACCAGCCTAAACGTTTCACCCC‑GGTCGCGTCTTCCCTCCCCTCAGGCCTGGGCGCGCTGGCCGACCAGGCTc                                                                                                >  1:10736320/1‑151 (MQ=255)
                                                        aCAGAACGGCGCGGTGG‑CCGTGGTGGGCAGGGAGTATTTCACGAGGCGACGCCGGATCGGGGAGGAAGTGCGACCAGCCTAAACGTTTCACCCC‑GGTCGCGTCTTCCCTCCCCTCAGGCCTGGGCGCGCTGGCCGACCAGGCTCAGCTCgg                                                                                         <  1:8509501/151‑1 (MQ=255)
                                                           gAACGGCGCGGTGG‑CCGTGGTGGGCAGGGAGTATTTCACGAGGCGACGCCGGATCGGGGAGGAAGTGCGACCAGCCTAAACGTTTCACCCC‑GGTCGCGTCTTCCCTCCCCTCAGGCCTGGGCGCGCTGGCCGACCAGGCTCAGCTCGGCGa                                                                                      >  1:10083916/1‑151 (MQ=255)
                                                            aaCGGCGCGGTGG‑CCGTGGTGGGCAGGGAGTATTTCACGAGGCGACGCCGGATCGGGGAGGAAGTGCGACCAGCCTAAACGTTTCACCCC‑GGTCGCGTCTTCCCTCCCCTCAGGCCTGGGCGCGCTGGCCGACCAGGCTCAGCTCGGCGAc                                                                                     >  2:10502986/1‑151 (MQ=255)
                                                            aaCGGCGCGGTGG‑CCGTGGTGGGCAGGGAGTATTTCACGAGGCGACGCCGGATCGGGGAGGAAGTGCGACCAGCCTAAACGTTTCACCCC‑GGTCGCGTCTTCCCTCCCCTCAGGCCTGGGCGCGCTGGCCGACCAGGCTCAGCTCGGCGAc                                                                                     <  2:890358/151‑1 (MQ=255)
                                                            aaCGGCGCGGTGG‑CCGTGGTGGGCAGGGAGTATTTCACGAGGCGACGCCGGATCGGGGAGGAAGTGCGACCAGCCTAAACGTTTCACCCC‑GGTCGCGTCTTCCCTCCCCTCAGGCCTGGGCGCGCTGGCCGACCAGGCTCAGCTCGGCGAc                                                                                     <  1:6224908/151‑1 (MQ=255)
                                                              cGGCGCGGTGG‑CCGTGGTGGGCAGGGAGTATTTCACGAGGCGACGCCGGATCGGGGAGGAAGTGCGACCAGCCTAAACGTTTCACCCC‑GGTCGCGTCTTCCCTCCCCTCAGGCCTGGGCGCGCTGGCCGACCAGGCTCAGCTCGGCGACCt                                                                                   <  1:3719762/151‑1 (MQ=255)
                                                                  gcgGTGG‑CCGTGGTGGGCAGGGAGTATTTCACGAGGCGACGCCGGATCGGGGAGGAAGTGCGACCAGCCTAAACGTTTCACCCC‑GGTCGCGTCTTCCCTCCCCTCAGGCCTGGGCGCGCTGGCCGACCAGGCTCAGCTCGGCGACCTtctc                                                                               <  1:10352719/151‑1 (MQ=255)
                                                                  gcgGTGG‑CCGTGGTGGGCAGGGAGTATTTCACGAGGCGACGCCGGATCGGGGAGGAAGTGCGACCAGCCTAAACGTTTCACCCC‑GGTCGCGTCTTCCCTCCCCTCAGGCCTGGGCGCGCTGGCCGACCAGGCTCAGCTCGGCGACCTtctc                                                                               <  1:7727371/151‑1 (MQ=255)
                                                                    ggTGG‑CCGTGGTGGGCAGGGAGTATTTCACGAGGCGACGCCGGATCGGGGAGGAAGTGCGACCAGCCTAACCGTTTCACCCC‑GGTCGCGTCTTCCCTCCCCTCAGGCCTGGGCGCGCTGGCCGACCAGGCTCAGCTCGGCGACCTTCTcgc                                                                             <  2:11518199/151‑1 (MQ=255)
                                                                      gtggccGTGGTGGGCAGGGAGTATTTCACGAGGCGACGCCGGATCGGGGAGGAAGTGCGACCAGCCTAAACGTTTCACCCC‑GGTCGCGTCTTCCCTCCCCTCAGGCCTGGGCGCGCTGGCCGACCAGGCTCAGCTCGGCGACCTTCTcgcg                                                                            >  2:6217644/5‑151 (MQ=255)
                                                                           cGTGGTGGGCAGGGAGTATTTCACGAGGCGACGCCGGATCGGGGAGGAAGTGCGACCAGCCTAAACGTTTCACCCC‑GGTCGCGTCTTCCCTCCCCTCAGGCCTGGGCGCGCTGGCCGACCAGGCTCAGCTCGGCGACCTTCTCGCGCCGCt                                                                       >  2:1306758/1‑151 (MQ=255)
                                                                            gtggtgGGCAGGGAGTATTTCACGAGGCGACGCCGGATCGGGGAGGAAGTGCGACCAGCCTAAACGTTTCACCCC‑GGTCGCGTCTTCCCTCCCCTCAGGCCTGGGCGCGCTGGCCGACCAGGCTCAGCTCGGCGACCTTCTCGCGCCGCTg                                                                      >  2:3932762/1‑151 (MQ=255)
                                                                             tggtggGCAGGGAGTATTTCACGAGGCGACGCCGGATCGGGGAGGAAGTGCGACCAGCCTAAACGTTTCACCCC‑GGTCGCGTCTTCCCTCCCCTCAGGCCTGGGCGCGCTGGCCGACCAGGCTCAGCTCGGCGACCTTCTCGCGCCGCTgc                                                                     >  1:8078038/1‑151 (MQ=255)
                                                                              ggtggGCAGGGAGTATTTCACGAGGCGACGCCGGATCGGGGAGGAAGTGCGACCAGCCTAAACGTTGCACCCC‑GGTCGCGACTTCCCTCCCCTCAGGCCTGGGCGCGCTGGCCGACCAGGCTCAGCTCGGCGACCTTCTCGCGCCGCTgcg                                                                    <  2:7142119/151‑1 (MQ=255)
                                                                                tggGCAGGGAGTATTTCACGAGGCGACGCCGGATCGGGGAGGAAGTGCGACCAGCCTAAACGTGTCACCCC‑GGTCGCGTCTTCCCTCCCCTCAGGCCTGGGCGCGCTGGCCGACCAGGCTCAGCTCGGCGACCTTCTCGCGCCGCTGCGcc                                                                  <  1:4954900/151‑1 (MQ=255)
                                                                                  ggCAGGGAGTATTTCACGAGGCGACGCCGGATCGGGGAGGAAGTGCGACCAGCCTAAACGTTTCACCCC‑GGTCGCGTCTTCCCTCCCCTCAGGCCTGGGCGCGCTGGCCGACCAGGCTCAGCTCGGCGACCTTCTCGCGCCGCTGCGCCAg                                                                >  2:2648243/1‑151 (MQ=255)
                                                                                   gCAGGGAGTATTTCACGAGGCGACGCCGGATCGGGGAGGAAGTGCGACCAGCCTAAACGTTTCACCCC‑GGTCGCGTCTTCCCTCCCCTCAGGCCTGGGCGCGCTGGCCGACCAGGCTCAGCTCGGCGACCTTCTCGCGCCGCTGCGCCAgg                                                               <  2:9734803/151‑1 (MQ=255)
                                                                                   gCAGGGAGTATTTCACGAGGCGACGCCGGATCGGGGAGGAAGTGCGACCAGCCTAAACGTTTCACCCC‑GGTCGCGTCTTCCCTCCCCTCAGGCCTGGGCGCGCTGGCCGACCAGGCTCAGCTCGGCGACCTTCTCGCGCCGCTGCGCCAgg                                                               >  2:3005345/1‑151 (MQ=255)
                                                                                    cAGGGAGTATTTCACGAGGCGACGCCGGATCGGGGAGGAAGTGCGACCAGCCTAAACGTTTCACCCC‑GGTCGCGTCTTCCCTCCCCTCAGGCCTGGGCGCGCTGGCCGACCAGGCTCAGCTCGGCGACCTTCTCGCGCCGCTGCGCCAGGt                                                              >  2:848841/1‑151 (MQ=255)
                                                                                     aGGGAGTATTTCACGAGGCGACGCCGGATCGGGGAGGAAGTGCGACCATCCTAAACGTTTCACCCC‑GGTCGCGTCTTCCCTCCCCTCAGGCCAGGGCGCGCTGGCCGACCAGGCGCAGCTCGGCCACCTTCTCGCGCCGCTGCGCCAggga                                                             >  2:4783026/1‑149 (MQ=255)
                                                                                     aGGGAGTATTTCACGAGGCGACGCCGGATCGGGGAGGAAGTGCGACCAGCCTAAACGTTTCACCCC‑GGTCGCGTCTTCCCTCCCCTCAGGCCTGGGCGCGCTGGCCGACCAGGCTCAGCTCGGCGACCTTCTCGCGCCGCTGCGCCAGGTa                                                             <  1:10171956/151‑1 (MQ=255)
                                                                                     aGGGAGTATTTCACGAGGCGACGCCGGATCGGGGAGGAAGTGCGACCAGCCTAAACGTTTCACCCC‑GGTCGCGTCTTCCCTCCCCTCAGGCCTGGGCGCGCTGGCCGACCAGGCTCAGCTCGGCGACCTTCTCGCGCCGCTGCGCCAGGTa                                                             >  2:3768448/1‑151 (MQ=255)
                                                                                      gggAGTATTTCACGAGGCGACGCCGGATCGGGGAGGACGTGCGACCAGCCTAAACGTTTCACCCC‑GGTCGCGTCTTCCCTCCCCTCAGGCCTGGGCGCGCTGGCCGACCAGGCTCAGCTCGGCGACCTTCTCGCGCCGCTGCGCCAGGTAg                                                            >  2:11183459/1‑151 (MQ=255)
                                                                                        gAGTATTTCACGAGGCGACGCCGGATCGGGGAGGAAGTGCGACCAGCCTAAACGTTTCACCCC‑GGTCGCGTCTTCCCTCCCCTCAGGCCTGGGCGCGCTGGCCGACCAGGCTCAGCTCGGCGACCTTCTCGCGCCGCTGCGCCAGGTAGCg                                                          <  2:182193/151‑1 (MQ=255)
                                                                                         aGTATTTCACGAGGCGACGCCGGATCGGGGAGGAAGTGCGACCAGCCTAAACGTTTCACCCC‑GGTCGCGTCTTCCCTCCCCTCAGGCCTGGGCGCGCTGGCCGACCAGGCTCAGCTCGGCGACCTTCTCGCGCCGCTGCGCCAGGTAGCgg                                                         <  1:256731/151‑1 (MQ=255)
                                                                                         aGTATTTCACGAGGCGACGCCGGATCGGGGAGGAAGTGCGACCAGCCTAAACGTTTCACCCC‑GGTCGCGTCTTCCCTCCCCTCAGGCCTGGGCGCGCTGGCCGACCAGGCTCAGCTCGGCGACCTTCTCGCGCCGCTGCGCCAGGTAGCgg                                                         >  1:5953229/1‑151 (MQ=255)
                                                                                           tATTTCACGAGGCGACGCCGGATCGGGGAGGAAGTGCGACCAGCCTAAACGTTTCACCCC‑GGTCGCGTCTTCCCTCCCCTCAGGCCTGGGCGCGCTGGCCGACCAGGCTCAGCTCGGCGACCTTCTCGCGCCGCTGCGCCAGGTAGCGGGt                                                       <  1:8956279/151‑1 (MQ=255)
                                                                                             tttCACGAGGCGACGCCGGATCGGGGAGGAAGTGCGACCAGCCTAAACGTTTCACCCC‑GGTCGCGTCTTCCCTCCCCTCAGGCCTGGGCGCGCTGGCCGACCAGGCTCAGCTCGGCGACCTTCTCGCGCCGCTGCGCCAGGTAGCGGGTGc                                                     <  1:5405887/151‑1 (MQ=255)
                                                                                             tttCACGAGGCGACGCCGGATCGGGGAGGAAGTGCGACCAGCCTAAACGTTTCACCCC‑GGTCGCGTCTTCCCTCCCCTCAGGCCTGGGCGCGCTGGCCGACCAGGCTCAGCTCGGCGACCTTCTCGCGCCGCTGCGCCAGGTAGCGGGTGc                                                     >  2:6658514/1‑151 (MQ=255)
                                                                                               tCACGAGGCGACGCCGGATCGGGGAGGAAGTGCGACCAGCCTAAACGTTTCACCCC‑GGTCGCGTCTTCCCTCCCCTCAGGCCTGGGCGCGCTGGCCGACCAGGCTCAGCTCGGCGACCTTCTCGCGCCGCTGCGCCAGGTAGCGGGTGCaa                                                   >  1:9252811/1‑151 (MQ=255)
                                                                                                cACGAGGCGACGCCGGATCGGGGAGGAAGTGCGACCAGCCTAAACGTTTCACCCC‑GGTCGCGTCTTCCCTCCCCTCAGGCCTGGGCGCGCTGGCCGACCAGGCTCAGCTCGGCGACCTTCTCGCGCCGCTGCGCCAGGTAGCGGGTGCAAc                                                  >  1:153486/1‑151 (MQ=255)
                                                                                                 aCGAGGCGACGCCGGATCGGGGAGGAAGTGCGACCAGCCTAAACGTTTCACCCC‑GGTCGCGTCTTCCCTCCCCTCAGGCCTGGGCGCGCTGGCCGACCAGGCTCAGCTCGGCGACCTTCTCGCGCCGCTGCGCCAGGTAGCGGGTGCAACt                                                 <  2:7512740/151‑1 (MQ=255)
                                                                                                    aGGCGACGCCGGATCGGGGAGGAAGTGCGACCAGCCTAAACGTTTCACCCC‑GGTCGCGTCTTCCCTCCCCTCAGGCCTGGGCGCGCTGGCCGACCAGGCTCAGCTCGGCGACCTTCTCGCGCCGCTGCGCCAGGTAGCGGGTGCAACTGac                                              >  1:6499517/1‑151 (MQ=255)
                                                                                                     ggCGACGCCGGATCGGGGAGGAAGTGCGACCAGCCTAAACGTTTCACCCC‑GGTCGCGTCTTCCCTCCCCTCAGGCCTGGGCGCGCTGGCCGACCAGGCTCAGCTCGGCGACCTTCTCGCGCCGCTGCGCCAGGTAGCGGGTGCAACTGaca                                             >  2:543981/1‑151 (MQ=255)
                                                                                                      gCGACGCCGGATCGGGGAGGAAGAGCGACCAGCCTAAACGTTTCACCCC‑GGTCGCGTCGTCCCTCCCCTCAGGCCTGGGCGCGCTGGCCGCCCAGGCTCAGCTCGGCGACATTCTCGCGCCGCTGCGCCAGGTAGCGGGTGCAACTGacac                                            <  2:2410300/151‑1 (MQ=255)
                                                                                                        gACGCCGGATCGGGGAGGAAGTGCGACCAGCCTAAACGTTTCACCCC‑GGTCGCGTCTTCCCTCCCCTCAGGCCTGGGCGCGCTGGCCGACCAGGCTCAGCTCGGCGACCTACTCGCGCCGCTGCGCCAGGTAGCGGGTGCAACTGACACGc                                          >  1:1765689/1‑151 (MQ=255)
                                                                                                           gCCGGATCGGGGAGGAAGTGCGACCAGCCTAAACGTTTCACCCC‑GGTCGCGTCTTCCCTCCCCTCAGGCCTGGGCGCGCTGGCCGACCAGGCTCAGCTCGGCGACCTTCTCGCGCCGCTGCGCCAGGTAGCGGGTGCAACTGACACGCagg                                       <  1:7497835/151‑1 (MQ=255)
                                                                                                           gCCGGATCGGGGAGGAAGTGCGACCAGCCTAAACGTTTCACCCC‑GGTCGCGTCTTCCCTCCCCTCAGGCCTGGGCGCGCTGGCCGACCAGGCTCAGCTCGGCGACCTTCTCGCGCCGCTGCGCCAGGTAGCGGGTGCAACTGACACGCagg                                       <  1:2999939/151‑1 (MQ=255)
                                                                                                             cGGATCGGGGAGGAAGTGCGACCAGCCTAAACGTTTCACCCC‑GGTCGCGTCTTCCCTCCCCTCAGGCCTGGGCGCGCTGGCCGACCAGGCTCAGCTCGGCGACCTTCTCGCGCCGCTGCGCCAGGTAGCGGGTGCAACTGACACGCaggaa                                     <  1:8769283/151‑1 (MQ=255)
                                                                                                              ggATCGGGGAGGAAGTGCGACCAGCCTAAACGTTTCACCCC‑GGTCGCGTCTTCCCTCCCCTCAGGCCTGGGCGCGCTGGCCGACCAGGCTCAGCTCGGCGACCTTCTCGCGCCGCTGCGCCAGGTAGCGGGTGCAACTGACACGCaggaag                                    >  2:4178957/1‑151 (MQ=255)
                                                                                                               gATCGGGGAGGAAGTGCGACCAGCCTAAACGTTTCACCCC‑GGTCGCGTCTTCCCTCCCCTCAGGCCTGGGCGCGCTGGCCGACCAGGCTCAGCTCGGCGACCTTCTCGCGCCGCTGCGCCAGGTAGCGGGTGCAACTGACACGCaggaagg                                   >  2:8675086/1‑151 (MQ=255)
                                                                                                               gATCGGGGAGGAAGTGCGACCAGCCTAAACGTTTCACCCC‑GGTCGCGTCTTCCCTCCCCTCAGGCCTGGGCGAGGTGGCCGACCAGGCTCAGCTCGGCGACCTTCTCGCGCCGCTGCGCCAGGTAGCGGGTGCAACTGACACGCaggacgg                                   >  2:2045974/1‑151 (MQ=255)
                                                                                                                aTCGGGGAGGAAGTGCGACCAGCCTAAACGTTTCACCCC‑GGTCGCGTCTTCCCTCCCCTCAGGCCTGGGCGCGCTGGCCGACCAGGCTCAGCTCGGCGACCTTCTCGCGCCGCTGCGCCAGGTAGCGGGTGCAACTGACACGCaggaagga                                  <  1:238812/151‑1 (MQ=255)
                                                                                                                aTCGGGGAGGAAGTGCGACCAGCCTAAACGTTTCACCCC‑GGTCGCGTCTTCCCTCCCCTCAGGCCTGGGCGCGCTGGCCGACCAGGCTCAGCTCGGCGACCTTCTCGCGCCGCTGCGCCAGGTAGCGGGTGCAACTGACACGCaggaagga                                  >  1:2798892/1‑151 (MQ=255)
                                                                                                                aTCGGGGAGGAAGTGCGACCAGCCTAAACGTTTCACCCC‑GGTCGCGTCTTCCCTCCCCTCAGGCCTGGGCGCGCTGGCCGACCAGGCTCAGCTCGGCGACCTTCTCGCGCCGCTGCGCCAGGTAGCGGGTGCAACTGACACGCaggaagga                                  <  1:3600476/151‑1 (MQ=255)
                                                                                                                 tCGGGGAGGAAGTGCGACCAGCCTAAACGTTTCACCCC‑GGTCGCGTCTTCCCTCCCCTCAGGCCTGGGCGCGCTGGCCGACCAGGCTCAGCTCGGCGACCTTCTCGCGCCGCTGCGCCAGGTAGCGGGTGCAACTGACACGCAGGAaggag                                 <  1:1105987/151‑1 (MQ=255)
                                                                                                                 tCGGGGAGGAAGTGCGACCAGCCTAAACGTTTCACCCC‑GGTCGCGTCTTCCCTCCCCTCAGGCCTGGGCGCGCTGGCCGACCAGGCTCAGCTCGGCGACCTTCTCGCGCCGCTGCGCCAGGTAGCGGGTGCAACTGACACGCAGGAaggag                                 <  2:678799/151‑1 (MQ=255)
                                                                                                                  cGGGGAGGAAGTGCGACCAGCCTAAACGTTTCACCCC‑GGTCGCGTCTTCCCTCCCCTCAGGCCTGGGCGCGCTGGCCGACCAGGCTCAGCTCGGCGACCTTCTCGCGCCGCTGCGCCAGGTAGCGGGTGCAACTGACACGCAGGAaggagg                                >  2:8796642/1‑151 (MQ=255)
                                                                                                                   ggggAGGAAGTGCGACCAGCCTAAACGTTTCACCCC‑GGTCGCGTCTTCCCTCCCCTCAGGCCTGGGCGCGCTGGCCGACCAGGCTCAGCTCGGCGACCTTCTCGCGCCGCTGCGCCAGGTAGCGGGTGCAACTGACACGCAGGAAGGAGGc                               >  2:650164/1‑151 (MQ=255)
                                                                                                                   ggggAGGAAGTGCGACCAGCCTAAACGTTTCACCCC‑GGTCGCGTCTTCCCTCCCCTCAGGCCTGGGCGCGCTGGCCGACCAGGCTCAGCTCGGCGACCTTCTCGCGCCGCTGCGCCAGGTAGCGGGTGCAACTGACACGCAGGAAGGAGGc                               >  2:598384/1‑151 (MQ=255)
                                                                                                                         gaAGTGCGACCAGCCTAAACGTTTCACCCC‑GGTCGCGTCTTCCCTCCCCTCAGGCCTGGGCGCGCTGGCCGACCAGGCTCAGCTCGGCGACCTTCTCGCGCCGCTGCGCCAGGTAGCGGGTGCAACTGACACGCAGGAAGGAGGCGAAGtt                         <  1:4944629/151‑1 (MQ=255)
                                                                                                                            gTGCGACCAGCCTAAACGTTTCACCCC‑GGTCGCGTCTTCCCTCCCCTCAGGCCTGGGCGCGCTGGCCGACCAGGCTCAGCTCGGCGACCTTCTCGCGCCGCTGCGCCAGGTAGCGGGTGCAACTGACACGCAGGAAGGAGGCGAAGTTGAg                      >  2:5683596/1‑151 (MQ=255)
                                                                                                                             tGCGACCAGCCTAAACGTTTCACCCC‑GGTCGCGTCTTCCCTCCCCTCAGGCCTGGGCGCGCTGGCCGACCAGGCTCAGCTCGGCGACCTTCTCGCGCCGCTGCGCCAGGTAGCGGGTGCAACTGACACGCAGGAAGGAGGCGAAGTTGAGc                     >  2:7854970/1‑151 (MQ=255)
                                                                                                                                 aCCAGCCTAAACGTTTCACCCC‑GGTCGCGTCTTCCCTCCCCTCAGGCCTTGGCGCGCTGGCCGACCAGGCTCAGCTCGGCGACCTTCTCGCGCCGCTGCGCCAGGTAGCGGGTGCAACTGACACGCAGGAAGGAGGCGAAGTTGATCAACt                 >  1:5532188/1‑151 (MQ=255)
                                                                                                                                 aCCAGCCTAAACGTTTCACCCC‑GGTCGCGACTTCCCTCCCCTCAGGCCTGGGCGCGCTGGCCGACCAGGCTCAGCTCGGCGACCTTCTCGCGCCGCTGCGCCAGGTAGCGGGTGCAACTGACACGCAGGAAGGAGGCGAAGTTGAGCACCt                 <  2:10237642/151‑1 (MQ=255)
                                                                                                                                  ccAGCCTAAACGTTTCACCCC‑GGTCGCGTCTTCCCTCCCCTCAGGCCTGGGCGCGCTGGCCGACCAGGCTCAGCTCGGCGACCTTCTCGCGCCGCTGCGCCAGGTAGCGGGTGCAACTGACACGCAGGAAGGAGGCGAAGTTGAGCACCTc                <  1:1308531/151‑1 (MQ=255)
                                                                                                                                  ccAGCCTAAACGTTTCACCCC‑GGTCGCGTCTTCCCTCCCCTCAGGCCTGGGCGCGCTGGCCGACCAGGCTCAGCTCGGCGACCTTCTCGCGCCGCTGCGCCAGGTAGCGGGTGCAACTGACACGCAGGAAGGAGGCGAAGTTGAGCACCTc                >  2:3201851/1‑151 (MQ=255)
                                                                                                                                   cAGCCTAAACGTTTCACCCC‑GGTCGCGTCTTCCCTCCCCTCAGGCCTGGGCGCGCTGGCCGACCAGGCTCAGCTCGGCGACCTTCTCGCGCCGCTGCGCCAGGTAGCGGGTGCAACTGACACGCAGGAAGGAGGCGAAGTTGAGCACCTcg               >  1:10961514/1‑151 (MQ=255)
                                                                                                                                     gCCTAAACGTTTCACCCC‑GGTCGCGTCTTCCCTCCCCTCAGGCCTGGGCGCGCTGGCCGACCAGGCTCAGCTCGGCGACCTTCTCGCGCCGCTGCGCCAGGTAGCGGGTGCAACTGACACGCAGGAAGGAGGCGAAGTTGAGCACCTcgcc             <  1:4652468/151‑1 (MQ=255)
                                                                                                                                     gCCTAAACGTTTCACCCC‑GGTCGCGTCTTCCCTCCCCTCAGGCCTGGGCGCGCTGGCCGACCAGGCTCAGCTCGGCGACCTTCTCGCGCCGCTGCGCCAGGTAGCGGGTGCAACTGACACGCAGGAAGGAGGCGAAGTTGAGCACCTcgcc             <  2:22444/151‑1 (MQ=255)
                                                                                                                                      ccTAAACGTTTCACCCC‑GGTCGCGTCTTCCCTCCCCTCAGGCCTGGGCGCGCTGGCCGACCAGGCTCAGCTCGGCGACCTTCTCGCGCCGCTGCGCCAGGTAGCGGGTGCAACTGACACGCAGGTAGGAGGCGAAGTTGAGCACCTcgccg            >  1:3915405/1‑151 (MQ=255)
                                                                                                                                      ccTAAACGTTTCACCCC‑GGTCGCGTCTTCCCTCCCCTCAGGCCTGGGCGCGCTGGCCGACCAGGCTCAGCTCGGCGACCTTCTCGCGCCGCTGCGCCAGGTAGCGGGTGCAACTGACACGCAGGAAGGAGGCGAAGTTGAGCACCTcgccg            <  2:8198625/151‑1 (MQ=255)
                                                                                                                                      ccTAAACGTTTCACCCC‑GGTCGCGTCTTCCCTCCCCTCAGGCCTGGGCGCGCTGGCCGACCAGGCTCAGCTCGGCGACCTTCTCGCGCCGCTGCGCCAGGTAGCGGGTGCAACTGACACGCAGGAAGGAGGCGAAGTTGAGCACCTcgccg            >  1:9901952/1‑151 (MQ=255)
                                                                                                                                        tAAACGTTTCACCCC‑GGTCGCGTCTTCCCTCCCCTCAGGCCTGGGCGCGCTGGCCGACCAGGCTCAGCTCGGCGACCTTCTCGCGCCGCTGCGCCAGGTAGCGGGTGCAACTGACACGCAGGAAGGAGGCGAAGTTGAGCACCTCGCcgcg          >  2:5816903/1‑151 (MQ=255)
                                                                                                                                        tAAACGTTTCACCCC‑GGTCGCGTCTTCCCTCCCCTCAGGCCTGGGCGCGCTGGCCGACCAGGCTCAGCTCGGCGACCTTCTCGCGCCGCTGCGCCAGGTAGCGGGTGCAACTGACACGCAGGAAGGAGGCGAAGTTGAGCACCTCGCcgcg          <  1:9418564/151‑1 (MQ=255)
                                                                                                                                          aaCGTTTCACCCC‑GGTCGCGTCTTCCCTCCCCTCAGGCCTGGGCGCGCTGGCCGACCAGGCTCAGCTCGGCGACCTTCTCGCGCCGCTGCGCCAGGTAGCGGGTGCAACTGACACGCAGGAAGGAGGCGAAGTTGAGCACCTCGCCGCGAt        >  2:6891102/1‑151 (MQ=255)
                                                                                                                                          aaCGTTTCACCCC‑GGTCGCGTCTTCCCTCCCCTCAGGCCTGGGCGCGCTGGCCGACCAGGCTCAGCTCGGCGACCTTCTCGCGCCGCTGCGCCAGGTAGCGGGTGCAACTGACACGCAGGAAGGAGGCGAAGTTGAGCACCTCGCCGCGAt        <  1:5035361/151‑1 (MQ=255)
                                                                                                                                          aaCGTTTCACCCC‑GGTCGCGTCTTCCCTCCCCTCAGGCCTGGGCGCGCTGGCCGACCAGGCTCAGCTCGGCGACCTTCTCGCGCCGCTGCGCCAGGTAGCGGGTGCAACTGACACGCAGGAAGGAGGCGAAGTTGAGCACCTCGCCGCGAt        >  1:5173293/1‑151 (MQ=255)
                                                                                                                                          aaCGTTTCACCCC‑GGTCGCGTCTTCCCTCCCCTCAGGCCTGGGCGCGCTGGCCGACCAGGCTCAGCTCGGCGACCTTCTCGCGCCGCTGCGCCAGGTAGCGGGTGCAACTGACACGCAGGAAGGAGGCGAAGTTGAGCACCTCGCCGCGAt        <  2:342088/151‑1 (MQ=255)
                                                                                                                                          aaCGTTTCACCCC‑GGTCGCGTCTTCCCTCCCCTCAGGCCTGGGCGCGCTGGCCGACCAGGCTCAGCTCGGCGACCTTCTCGCGCCGCTGCGCCAGGTAGCGGGTGCAACTGACACGCAGGAAGGAGGCGAAGTTGAGCACCTCGCCGCGAt        >  1:11595596/1‑151 (MQ=255)
                                                                                                                                          aaCGTTTCACCCC‑GGTCGCGTCGTCCCTCCCCTCAGGCCTGGGCGCGCTGGCCGACCAGGCTCAGCTCGGCGACCTTCTCGCGCCGCTGCGCCAGGTAGCGGGTGCAACTGACACGCAGGAAGGAGGCGAAGTTGAGCACCTCGCCGCGAt        <  2:1383801/151‑1 (MQ=255)
                                                                                                                                            cGTTTCACCCC‑GGTCGCGTCTTCCCTCCCCTCAGGCCTGGGCGCGCTGGCCGACCAGGCTCAGCTCGGCGACCTTCTCGCGCCGCTGCGCCAGGTAGCGGGTGCAACTGACACGCAGGAAGGAGGCGAAGTTGAGCACCTCGCCGCGATAg      <  1:11092227/151‑1 (MQ=255)
                                                                                                                                             gTTTCACCCC‑GGTCGCGTCTTCCCTCCCCTCAGGCCTGGGCGCGCTGGCCGACCAGGCTCAGCTCGGCGACCTTCTCGCGCCGCTGCGCCAGGTAGCGGGTGCCACTGACACGCAGGAAGGAGGCGAAGTTGAGCACCTCGCCGCGATAGt     >  1:7822423/1‑151 (MQ=255)
                                                                                                                                                       |                                                                                                                                                
TTGCGCAGCGGCGAGGCGTCGGCCGGCACCGCCACGCTGTGGCTGACCGCCGAGGGACAGAACGGCGCGGTGGCCCGTGGTGGGCAGGGAGTATTTCACGAGGCGACGCCGGATCGGGGAGGAAGTGCGACCAGCCTAAACGTTTCACCCCGGGTGGGTTCTTCCCTCCCCTCAGGCCTGGGCGCGCTGGCCGACCAGGCTCAGCTCGGCGACCTTCTCGCGCCGCTGCGCCAGGTAGCGGGTGCAACTGACACGCAGGAAGGAGGCGAAGTTGAGCACCTCGCCGCGATAGTCCA  >  NC_008463/5028044‑5028339

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A