Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A0 F0 I1 R1
|
145 |
28.3 |
2389566 |
89.0% |
2126713 |
107.1 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
AM260479 |
3,615,722 |
+A |
intergenic (+48/‑141) |
prfA → / → h16_A3341 |
protein chain release factor A (RF‑1)/Hypothetical protein |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | AM260479 | 3,615,720 | 1 | . | A | 100.0%
| 154.0
/ NA
| 41 | intergenic (+46/‑143) | prfA/h16_A3341 | protein chain release factor A (RF‑1)/Hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base . (0/0); new base A (19/22); total (19/22) |
GCGACATGGATGAGCTGCTGTCGGCACTGGCTTCGGAACACCAGGCTGATCAGCTTGCTGCACTTGGCGAAGAGTCGTAAGCACCACAAAATGTCGTGCAACCGGCCTTCCGCCCCGAACA‑CGCGT‑AAGTCGCCGATATCTTTGTATTTCTTGTAAATGCTTGAAACAGCACTGGTAGGGGTAAGGCGGCTGTCTATAATCGATTTCGACCGGCAAACGGAGAATGGTGCCGGGTAGTCACTCCCGA > AM260479/3615595‑3615841
|
gCGACATGGATGAGCTGCTGTCGGCACTGGCTTCGGAACACCAGGCTGATCAGCTTGCTGCACTTGGCGAAGAGTCGTAAGCACCACAAAATGTCGTGCAACCGGCCTTCCGCCCCGAACACCGCGT‑aaagtcg < 2:289303/134‑6 (MQ=255)
aTGGATGAGCTGCTGTCGGCACTGGCTTCGGAACACCAGGCTGATCAGCTTGCTGCACTTGGCGAAGAGTCGTAAGCACCACAAAATGTCGTGCAACCGGCCTTCCGCCCCGAACACCGCGTAAAGTCGCCGat < 2:240190/134‑1 (MQ=255)
aTGGATGAGCTGCTGTCGGCACTGGCTTCGGAACACCAGGCTGATCAGCTTGCTGCACTTGGCGAAGAGTCGTAAGCACCACAAAATGTCGTGCAACCGGCCTTCCGCCCCGAACACCGCGTAAAGTCGCCGa > 2:910692/1‑133 (MQ=255)
aTGGATGAGCTGCTGTCGGCACTGGCTTCGGAACACCAGGCTGATCAGCTTGCTGCACTTGGCGAAGAGTCGTAAGCACCACAAAATGTCGTGCAACCGGCCTTCCGCCCCGAACACCGCGTAAAGTCGCCGa > 2:444645/1‑133 (MQ=255)
aTGGATGAGCTGCTGTCGGCACTGGCTTCGGAACACCAGGCTGATCAGCTTGCTGCACTTGGCGAAGAGTCGTAAGCACCACAAAATGTCGTGCAACCGGCCTTCCGCCCCGAACACCGCGTAAAGTCGCCGa > 2:331279/1‑133 (MQ=255)
tGAGCTGCTGTCGGCACTGGCTTCGGAACACCAGGCTGATCAGCTTGCTGCACTTGGCGAAGAGTCGTAAGCACCACAAAATGTCGTGCAACCGGCCTTCCGCCCCGAACACCGCGTAAAGTCGCCGATATCtt < 2:218656/134‑1 (MQ=255)
aCTGGCTTCGGAACACCAGGCTGATCAGCTTGCTGCACTTGGCGAAGAGTCGTAAGCACCACAAAATGTCGTGCAACCGGCCTTCCGCCCCGAACACCGCGTAAAGTCGCCGATATCTTTGTATTTCTTGTaaa < 1:910692/134‑1 (MQ=255)
aCTGGCTTCGGAACACCAGGCTGATCAGCTTGCTGCACTTGGCGAAGAGTCGTAAGCACCACAAAATGTCGTGCAACCGGCCTTCCGCCCCGAACACCGCGTAAAGTCGCCGATATCTTTGTATTTCTTGTaaa < 1:477333/134‑1 (MQ=255)
cTTCGGAACACCAGGCTGATCAGCTTGCTGCACTTGGCGAAGAGTCGTAAGCACCACAAAATGTCGTGCAACCGGCCTTCCGCCCCGAACACCGCGTAAAGTCGCCGATATCTTTGTATTTCTTGTAAATGCtt < 1:1189067/134‑1 (MQ=255)
cTTCGGAACACCAGGCTGATCAGCTTGCTGCACTTGGCGAAGAGTCGTAAGCACCACAAAATGTCGTGCAACCGGCCTTCCGCCCCGAACACCGCGTAAAGTCGCCGATATCTTTGTATTTCTTGTAAATGCtt < 1:444645/134‑1 (MQ=255)
gAACACCAGGCTGATCAGCTTGCTGCACTTGGCGAAGAGTCGTAAGCACCACAAAATGTCGTGCAACCGGCCTTCCGCCCCGAACACCGCGTAAAGTCGCCGATATCTTTGTATTTCTTGTAAATGCTTGAAAc < 2:899152/134‑1 (MQ=255)
gCTTGCTGCACTTGGCGAAGAGTCGTAAGCACCACAAAATGTCGTGCAACCGGCCTTCCGCCCCGAACACCGCGTAAAGTCGCCGATATCTTTg > 2:709237/1‑94 (MQ=255)
gCTTGCTGCACTTGGCGAAGAGTCGTAAGCACCACAAAATGTCGTGCAACCGGCCTTCCGCCCCGAACACCGCGTAAAGTCGCCGATATCTTTg < 1:709237/94‑1 (MQ=255)
tGGCGAAGAGTCGTAAGCACCACAAAATGTCGTGCAACCGGCCTTCCGCCCCGAACACCGCGTAAAGTCGCCGATATCTTTGTATTTCTTGTAAATGCtt > 2:1194748/1‑100 (MQ=255)
tGGCGAAGAGTCGTAAGCACCACAAAATGTCGTGCAACCGGCCTTCCGCCCCGAACACCGCGTAAAGTCGCCGATATCTTTGTATTTCTTGTAAATGCtt < 1:1194748/100‑1 (MQ=255)
aaGAGTCGTAAGCACCACAAAATGTCGTGCAACCGGCCTTCCGCCCCGAACACCGCGTAAAGTCGCCGATATCTTTGTATTTCTTGTAAATGCTTGAAACAGCACTGGTAGGGGTAAg > 1:204834/1‑118 (MQ=255)
aaGAGTCGTAAGCACCACAAAATGTCGTGCAACCGGCCTTCCGCCCCGAACACCGCGTAAAGTCGCCGATATCTTTGTATTTCTTGTAAATGCTTGAAACAGCACTGGTAGGGGTAAg < 2:204834/118‑1 (MQ=255)
tCGTAAGCACCACAAAATGTCGTGCAACCGGCCTTCCGCCCCGAACACCGCGTAAAGTCGCCGATATCTTTGTATTTCTTGTAAATGCTTGAAACAGCACTGGTAGGGGTAAGGCGGCTGTCTATAATCGAttt > 1:302299/1‑134 (MQ=255)
tAAGCACCACAAAATGTCGTGCAACCGGCCTTCCGCCCCGAACACCGCGTAAAGTCGCCGATATCTTTGTATTTCTTGTAAATGCTTGAAACAGCACTGGTAGGGGTAAggc > 2:424282/1‑112 (MQ=255)
tAAGCACCACAAAATGTCGTGCAACCGGCCTTCCGCCCCGAACACCGCGTAAAGTCGCCGATATCTTTGTATTTCTTGTAAATGCTTGAAACAGCACTGGTAGGGGTAAggc < 1:424282/112‑1 (MQ=255)
ccacAAAATGTCGTGCAACCGGCCTTCCGCCCCGAACACCGCGTAAAGTCGCCGATATCTTTGTATTTCTTGTAAATGCTTGAAACAGCACTGGTAGGGGTAAGGCGGCTGTCTATAATCGATTTCGACCGGCa < 2:302299/134‑1 (MQ=255)
caAAATGTCGTGCAACCGGCCTTCCACCCCGAACACCGCGTAAAGTCGCCGATATCTTTGTATTTCTTGTAAATGCTTGAAACAGCACTGGTAGGGGTAAg < 2:424662/101‑1 (MQ=255)
caAAATGTCGTGCAACCGGCCTTCCACCCCGAACACCGCGTAAAGTCGCCGATATCTTTGTATTTCTTGTAAATGCTTGAAACAGCACTGGTAGGGGTAAg > 1:424662/1‑101 (MQ=255)
aaaTGTCGTGCAACCGGCCTTCCGCCCCGAACACCGCGTAAAGTCGCCGATATCTTTGTATTTCTTGTAAATGCTTGAAACAGCACTGGTAGGGGTAAg > 1:151071/1‑99 (MQ=255)
aaaTGTCGTGCAACCGGCCTTCCGCCCCGAACACCGCGTAAAGTCGCCGATATCTTTGTATTTCTTGTAAATGCTTGAAACAGCACTGGTAGGGGTAAg > 2:731726/1‑99 (MQ=255)
aaaTGTCGTGCAACCGGCCTTCCGCCCCGAACACCGCGTAAAGTCGCCGATATCTTTGTATTTCTTGTAAATGCTTGAAACAGCACTGGTAGGGGTAAg < 2:151071/99‑1 (MQ=255)
aaaTGTCGTGCAACCGGCCTTCCGCCCCGAACACCGCGTAAAGTCGCCGATATCTTTGTATTTCTTGTAAATGCTTGAAACAGCACTGGTAGGGGTAAg < 1:731726/99‑1 (MQ=255)
aTGTCGTGCAACCGGCCTTCCGCCCCGAACACCGCGTAAAGTCGCCGATATCTTTGTATTTCTTGTAAATGCTTGAAACAGCACTGGTAGGGGTAAGGCGGCTGTCTATAATCGATTTCGAc > 2:129740/1‑122 (MQ=255)
aTGTCGTGCAACCGGCCTTCCGCCCCGAACACCGCGTAAAGTCGCCGATATCTTTGTATTTCTTGTAAATGCTTGAAACAGCACTGGTAGGGGTAAGGCGGCTGTCTATAATCGATTTCGAc < 1:129740/122‑1 (MQ=255)
tCGTGCAACCGGCCTTCCGCCCCGAACACCGCGTAAAGTCGCCGATATCTTTGTATTTCTTGTAAATGCTTGAAACAGCACTGGTAGGGGTAAGGCGGCTGTCTATAATCGATTTCGACCGGCAAACGGAGAAt < 1:607950/134‑1 (MQ=255)
gTGCAACCGGCCTTCCGCCCCGAACACCGCGTAAAGTCGCCGATATCTTTGTATTTCTTGTAAATGCTTGAAACAGCACTGATAGGGGTAAGGCGGCTGTCTATAATCGATTTCGACCGGCAAACGGAGAATgg > 1:1027539/1‑134 (MQ=255)
cAACCGGCCTTCCGCCCCGAACACCGCGTAAAGTCGCCGATATCTTTGTATTTCTTGTAAATGCTTGAAACAGCACTGGTAGGGGTAAGGCGGCTGTCTATAATCGATTTCGACCg > 1:167502/1‑116 (MQ=255)
cAACCGGCCTTCCGCCCCGAACACCGCGTAAAGTCGCCGATATCTTTGTATTTCTTGTAAATGCTTGAAACAGCACTGGTAGGGGTAAGGCGGCTGTCTATAATCGATTTCGACCg < 2:167502/116‑1 (MQ=255)
aCCGGCCTTCCGCCCCGAACACCGCGTAAAGTCGCCGATATCTTTGTATTTCTTGTAAATGCTTGAAACAGCACTGGTAGGGGTAAGGCGGCTGTCTATAATCGATTTCGACCGGCAAACGGAGAATGGTGCCg > 2:194610/1‑134 (MQ=255)
cGCCCCGAACACCGCGTAAAGTCGCCGATATCTTTGTATTTCTTGTAAATGCTTGAAACAGCACTGGTAGGGGTAAGGCGGCTGTCTATAATCGATTTCGACCGGCAAACGGAGAATGGTGCCGGGTAGTCACt > 1:551094/1‑134 (MQ=255)
cGAACACCGCGTAAAGTCGCCGATATCTTTGTATTTCTTGTAAATGCTTGAAACAGCACTGGTAGGGGTAAGGCGGCTGTCTATAATCGATTTCGACCGGCAAACGGAGAATGGTGCCGGGTAGTCACTCCCga < 1:331279/134‑1 (MQ=255)
ccgcgTAAAGTCGCCGATATCTTTGTATTTCTTGTAAATGCTTGAAACAGCACTGGTAGGGGTAAg > 2:510026/2‑66 (MQ=255)
ccgcgTAAAGTCGCCGATATCTTTGTATTTCTTGTAAATGCTTGAAACAGCACTGGTAGGGGTAAg > 2:502250/2‑66 (MQ=255)
ccgcgTAAAGTCGCCGATATCTTTGTATTTCTTGTAAATGCTTGAAACAGCACTGGTAGGGGTAAg < 1:510026/65‑1 (MQ=255)
ccgcgTAAAGTCGCCGATATCTTTGTATTTCTTGTAAATGCTTGAAACAGCACTGGTAGGGGTAAg < 1:931155/65‑1 (MQ=255)
ccgcgTAAAGTCGCCGATATCTTTGTATTTCTTGTAAATGCTTGAAACAGCACTGGTAGGGGTAAg < 1:502250/65‑1 (MQ=255)
ccgcgTAAAGTCGCCGATATCTTTGTATTTCTTGTAAATGCTTGAAACAGCACTGGTAGGGGTAAg > 2:931155/2‑66 (MQ=255)
|
GCGACATGGATGAGCTGCTGTCGGCACTGGCTTCGGAACACCAGGCTGATCAGCTTGCTGCACTTGGCGAAGAGTCGTAAGCACCACAAAATGTCGTGCAACCGGCCTTCCGCCCCGAACA‑CGCGT‑AAGTCGCCGATATCTTTGTATTTCTTGTAAATGCTTGAAACAGCACTGGTAGGGGTAAGGCGGCTGTCTATAATCGATTTCGACCGGCAAACGGAGAATGGTGCCGGGTAGTCACTCCCGA > AM260479/3615595‑3615841
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
BRESEQ :: bam2aln output
GATCATGGACGGCGACATGGATGAGCTGCTGTCGGCACTGGCTTCGGAACACCAGGCTGATCAGCTTGCTGCACTTGGCGAAGAGTCGTAAGCACCACAAAATGTCGTGCAACCGGCCTTCCGCCCCGAACA‑CGCGT‑AAGTCGCCGATATCTTTGTATTTCTTGTAAATGCTTGAAACAGCACTGGTAGGGGTAAGGCGGCTGTCTATAATCGATTTCGACCGGCAAACGGAGAATGGTGCCGGGTAGTCACTCCCGAGAACATACCTAGGAC > AM260479/3615584‑3615856
|
GATCATGGACGGCGACATGGATGAGCTGCTGTCGGCACTGGCTTCGGAACACCAGGCTGATCAGCTTGCTGCACTTGGCGAAGAGTCGTAAGCACCACAAAATGTCGTGCAACCGGCCTTCCGCCCCGAACACCGCGTAAAGTCGCCGAT < GWNJ‑0478:712:GW2002102894th:2:2205:20512:3868/150‑1 (MQ=60)
tgtttactttttttttttttcaagcagaagacggcatacgagattagcgagtgtctcgtgggctcggagatgtgtataagagacagACAAAATGTCGTGCAACCGGCCTTCCGCCCCGAACACCGCGTAAAGTCGCCGATATCTTTGTA < GWNJ‑0478:712:GW2002102894th:2:1201:10469:32610/63‑1 (MQ=60)
cgagtgtctcgtgggctcggagatgtgtataagagacagCACCAGGCTGATCAGCTTGCTGCACTTGGCGAAGAGTCGTAAGCACCACAAAATGTCGTGCAACCGGCCTTCCGCCCCGAACACCGCGTAAAGTCGCCGATATCTTTGTAT < GWNJ‑0478:712:GW2002102894th:2:2106:16194:67488/111‑1 (MQ=60)
ggctcggagatgtgtataagagacagACACCAGGCTGATCAGCTTGCTGCACTTGGCGAAGAGTCGTAAGCACCACAAAATGTCGTGCAACCGGCCTTCCGCCCCGAACACCGCGTAAAGTCGCCGATATCTTTGTATTTCTTGTAAATG < GWNJ‑0478:712:GW2002102894th:2:2106:17418:58473/124‑1 (MQ=60)
CACTGGCTTCGGAACACCAGGCTGATCAGCTTGCTGCACTTGGCGAAGAGTCGTAAGCACCACAAAATGTCGTGCAACCGGCCTTCCGCCCCGAACACCGCGTAAAGTCGCCGATATCTTTGTATTTCTTGTAAATGCTTGAAACAGCAC < GWNJ‑0478:712:GW2002102894th:2:2203:2597:4410/150‑1 (MQ=60)
CACTGGCTTCGGAACACCAGGCTGATCAGCTTGCTGCACTTGGCGAAGAGTCGTAAGCACCACAAAATGTCGTGCAACCGGCCTTCCGCCCCGAACACCGCGTAAAGTCGCCGATATCTTTGTATTTCTTGTAAATGCTTGAAACAGCAC < GWNJ‑0478:712:GW2002102894th:2:1210:5669:23154/150‑1 (MQ=60)
gagatgtgtataagagacagTCAGCTTGCTGCACTTGGCGAAGAGTCGTAAGCACCACAAAATGTCGTGCAACCGGCCTTCCGCCCCGAACACCGCGTAAAGTCGCCGATATCTTTGTATTTCTTGTAAATGCTTGAAACAGCACTGGTA < GWNJ‑0478:712:GW2002102894th:2:2216:3207:101041/130‑1 (MQ=60)
GCTTCGGAACACCAGGCTGATCAGCTTGCTGCACTTGGCGAAGAGTCGTAAGCACCACAAAATGTCGTGCAACCGGCCTTCCGCCCCGAACACCGCGTAAAGTCGCCGATATCTTTGTATTTCTTGTAAATGCTTGAAACAGCACTGGTA < GWNJ‑0478:712:GW2002102894th:2:1209:2278:57832/150‑1 (MQ=60)
GCTTCGGAACACCAGGCTGATCAGCTTGCTGCACTTGGCGAAGAGTCGTAAGCACCACAAAATGTCGTGCAACCGGCCTTCCGCCCCGAACACCGCGTAAAGTCGCCGATATCTTTGTATTTCTTGTAAATGCTTGAAACAGCACTGGTA < GWNJ‑0478:712:GW2002102894th:2:2216:3184:73781/150‑1 (MQ=60)
ggcatacgagattagcgagtgtctcgtgggctcggagatgtgtataagagacagCTTCCGCCCCGAACACCGCGTAAAGTCGCCGATATCTTTGTATTTCTTGTAAATGCTTGAAACAGCACTGGTAGGGGTAAGGCGGCTGTCTATAAT < GWNJ‑0478:712:GW2002102894th:2:1213:20169:83272/96‑1 (MQ=60)
ggcatacgagattagcgagtgtctcgtgggctcggagatgtgtataagagacagCTTCCGCCCCGAACACCGCGTAAAGTCGCCGATATCTTTGTATTTCTTGTAAATGCTTGAAACAGCACTGGTAGGGGTAAGGCGGCTGTCTATAAT < GWNJ‑0478:712:GW2002102894th:2:1213:5539:47880/96‑1 (MQ=60)
ggcatacgagattagcgagtgtctcgtgggctcggagatgtgtataagagacagCTTCCGCCCCGAACACCGCGTAAAGTCGCCGATATCTTTGTATTTCTTGTAAATGCTTGAAACAGCACTGGTAGGGGTAAGGCGGCTGTCTATAAT < GWNJ‑0478:712:GW2002102894th:2:2202:2298:27213/96‑1 (MQ=60)
ggagatgtgtataagagacagGTCGTAAGCACCACAAAATGTCGTGCAACCGGCCTTCCGCCCCGAACACCGCGTAAAGTCGCCGATATCTTTGTATTTCTTGTAAATGCTTGAAACAGCACTGGTAGGGGTAAGGCGGCTGTCTATAAT < GWNJ‑0478:712:GW2002102894th:2:2108:1711:82946/129‑1 (MQ=60)
agagacagCTTGGCGAAGAGTCGTAAGCACCACAAAATGTCGTGCAACCGGCCTTCCGCCCCGAACACCGCGTAAAGTCGCCGATATCTTTGTATTTCTTGTAAATGCTTGAAACAGCACTGGTAGGGGTAAGGCGGCTGTCTATAATCG < GWNJ‑0478:712:GW2002102894th:2:1208:9188:51144/142‑1 (MQ=60)
TTGCTGCACTTGGCGAAGAGTCGTAAGCACCACAAAATGTCGTGCAACCGGCCTTCCGCCCCGAACACCGCGTAAAGTCGCCGATATCTTTGTATTTCTTGTAAATGCTTGAAACAGCACTGGTAGGGGTAAGGCGGCTGTCTATAATCT > GWNJ‑0478:712:GW2002102894th:2:1113:3424:56581/1‑150 (MQ=60)
GCACTTGGCGAAGAGTCGTAAGCACCACAAAATGTCGTGCAACCGGCCTTCCGCCCCGAACACCGCGTAAAGTCGCCGATATCTTTGTATTTCTTGTAAATGCTTGAAACAGCACTGGTAGGGGTAAGGCGGCTGTCTATAATCGATTTC > GWNJ‑0478:712:GW2002102894th:2:1201:14578:48173/1‑150 (MQ=60)
GCGAAGAGTCGTAAGCACCACAAAATGTCGTGCAACCGGCCTTCCGCCCCGAACACCGCGTAAAGTCGCCGATctgtctcttatacacatctccgagcccacgagacactcgctaatctcgtatgccgtcttctgcttgaaaaaaaaaa > GWNJ‑0478:712:GW2002102894th:2:1204:9411:29434/1‑73 (MQ=60)
GAGTCGTAAGCACCACAAAATGTCGTGCAACCGGCCTTCCACCCCGAACACCGCGTAAAGTCGCCGATATCTTTGTATTTCTTGTAAATGCTTGAAACAGCACTGGTAGGGGTAAGGCGGCTGTCTATAATCtgtctcttatacacatct > GWNJ‑0478:712:GW2002102894th:2:1208:19247:53039/1‑132 (MQ=60)
GTCGTAAGCACCACAAAATGTCGTGCAACCGGCCTTCCGCCCCGAACACCGCGTAAAGTCGCCGATATCTTTGTATTTCTTGTAAATGCTTGAAACAGCACTGGTAGGGGTAAGGCGGCTGTCTATAATCtgtctcttatacacatctcc > GWNJ‑0478:712:GW2002102894th:2:1109:19972:81500/1‑130 (MQ=60)
AAGCACCACAAAATGTCGTGCAACCGGCCTTCCGCCCCGAACACCGCGTAAAGTCGCCGATATCTTTGTATTTCTTGTAAATGCTTGAAACAGCACTGGTAGGGGTAAGGCGGCTGTCTATAATCGATTTCGACCGGCAAACGGAGAAT < GWNJ‑0478:712:GW2002102894th:2:1108:14694:66579/149‑1 (MQ=60)
GCACCACAAAATGTCGTGCAACCGGCCTTCCGCCCCGAACACCGCGTAAAGTCGCCGATATCTTTGTATTTCTTGTAAATGCTTGAAACAGCACTGATAGGGGTAAGGCGGCTGTCTATAATCGATTTCGACCGGCAAACGGAGAATGGT > GWNJ‑0478:712:GW2002102894th:2:2207:2618:77293/1‑150 (MQ=60)
CCACAAAATGTCGTGCAACCGGCCTTCCGCCCCGAACACCGCGTAAAGTCGCCGATATCTTTGTATTTCTTGTAAATGCTTGAAACAGCACTGGTAGGGGTAAGGCGGCTGTCTATAATCGATTTCGACCGGCAAACGGAGAATGGctgt > GWNJ‑0478:712:GW2002102894th:2:1111:20488:66740/1‑146 (MQ=60)
GTCGTGCAACCGGCCTTCCGCCCCGAACACCGCGTAAAGTCGCCGATATCTTTGTATTTCTTGTAAATGCTTGAAACAGCACTGGTAGGGGTAAGGCGGCTGTCTATAATCGATTTCGACCGGCAAACGGAGAATGGTGCCGGGTAGTCA < GWNJ‑0478:712:GW2002102894th:2:2101:10617:54212/150‑1 (MQ=60)
GTGCAACCGGCCTTCCGCCCCGAACACCGCGTAAAGTCGCCGATATCTTctgtctcttatacacatctccgagcccacgagacactcgctaatctcgtatgccgtcttctgcttgaaaaaaaaaaataaatactaaaacaacttaaattg > GWNJ‑0478:712:GW2002102894th:2:1109:20317:11016/1‑49 (MQ=60)
GTGCAACCGGCCTTCCGCCCCGAACACCGCGTAAAGTCGCCGATATCTTTGTATTTCTTGTAAATGCTTGAAACAGCACTGGTAGGGGTAAGGCGGCTGTCTATAATCGATTTCGACCGGCAAACGGAGAATGGTGCCGGGTAGTCACTC > GWNJ‑0478:712:GW2002102894th:2:1215:15417:74412/1‑150 (MQ=60)
CCCGAACACCGCGTAAAGTCGCCGATATCTTTGTATTTCTTGTAAATGCTTGAAACAGCACTGGTAGGGGTAAGGCGGCTGTCTATAATCctgtctcttatacacatctccgagcccacgagacactcgctaatctcgtatgccgtcttc > GWNJ‑0478:712:GW2002102894th:2:1114:8233:60799/1‑90 (MQ=60)
CCGAACACCGCGTAAAGTCGCCGATATCTTTGTATTTCTTGTAAATGCTTGAAACAGCACTGGTAGGGGTAAGGCGGCTGTCTATAATCGATTTCGACCGGCAAACGGAGAATGGTGCCGGGTAGTCACTCCCGAGAACATACCTAGGAC < GWNJ‑0478:712:GW2002102894th:2:1202:11902:24576/150‑1 (MQ=60)
|
GATCATGGACGGCGACATGGATGAGCTGCTGTCGGCACTGGCTTCGGAACACCAGGCTGATCAGCTTGCTGCACTTGGCGAAGAGTCGTAAGCACCACAAAATGTCGTGCAACCGGCCTTCCGCCCCGAACA‑CGCGT‑AAGTCGCCGATATCTTTGTATTTCTTGTAAATGCTTGAAACAGCACTGGTAGGGGTAAGGCGGCTGTCTATAATCGATTTCGACCGGCAAACGGAGAATGGTGCCGGGTAGTCACTCCCGAGAACATACCTAGGAC > AM260479/3615584‑3615856
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
