Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F1 I1 R1
|
152 |
24.9 |
2083062 |
91.6% |
1908084 |
109.2 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
AM260479 |
1,231,226 |
2 bp→CA |
intergenic (+63/‑151) |
glnE → / → h16_A1128 |
glutamine synthetase adenylyltransferase/Membrane protein |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | AM260479 | 1,231,226 | 0 | A | C | 100.0%
| 78.6
/ NA
| 22 | intergenic (+63/‑152) | glnE/h16_A1128 | glutamine synthetase adenylyltransferase/Membrane protein |
| Reads supporting (aligned to +/- strand): ref base A (0/0); new base C (10/12); total (10/12) |
| * | AM260479 | 1,231,227 | 0 | C | A | 100.0%
| 79.4
/ NA
| 22 | intergenic (+64/‑151) | glnE/h16_A1128 | glutamine synthetase adenylyltransferase/Membrane protein |
| Reads supporting (aligned to +/- strand): ref base C (0/0); new base A (10/12); total (10/12) |
TCGAATTCACGGGTGCAGTACCTGGTGCTGCTGCACAACCCGGGCGCACCCGCAGCTGACGCGCAACGCGGGGCAATATCGCGCTGCTGCGCGCAGCGGGCGAGCTGGGCCTGATCGACGCACCGCTGGCACTGGCGGTGGGCGATGCCTACCGGCTGTTCCGCGCGCGCCAGCACCAGCTGCGGCTCGAGGGGCAGGCGCATGCGCGCGTCGCGCCCGAGTCGGTGGCGCAGCAGACCGGACAGGT > AM260479/1231105‑1231351
||
tCGAATTCAC‑GGTGCAGTACCTGGTGCTGCTGCAC‑AGCCGGGCGCACCCGCAGCTGACGCGCAACGC‑GGGCAATATCGCGCTGCTGCGCGCAGCGGGCGAGCTGGGCCTGATCGACGCCACGCTGGCACTGGCg > 1:97127/1‑134 (MQ=255)
cggTGCAGTACCTGGTGCTGCTGCAC‑AGCCGGGCGCACCCGCAGCTGACGCGCAACGC‑GGGCAATATCGCGCTGCTGCGCGCAGCGGGCGAGCTGGGCCTGATCGACGCCACGCTGGCACTGGCGGTGGGCGAt < 1:354377/133‑1 (MQ=255)
gTGCAGTACCTGGTGCTGCTGCAC‑AGCCGGGCGCACCCGCAGCTGACGCGCAACGC‑GGGCAATATCGCGCTGCTGCGCGCAGCGGGCGAGCTGGGCCTGATCGACGCCACGCTGGCACTGGCGGTGGGCGATGc < 2:97127/134‑1 (MQ=255)
tgctgctgcAC‑AGCCGGGCGCACCCGCAGCTGACGCGCAACGC‑GGGCAATATCGCGCTGCTGCGCGCAGCGGGCGAGCTGGGCCTGATCGACGCCACGCTGGCACTGGCGGTGGGCGATGCCTACCGGCTGTTc < 2:223529/134‑1 (MQ=255)
ctgctgcAC‑AGCCGGGCGCACCCGCAGCTGACGCGCAACGC‑GGGCAATATCGCGCTGCTGCGCGCAGCGGGCGAGCTGGGCCTGATCGACGCCACGCTGGCACTGGCGGTGGGCGATGCCTACCGGCTGTTCcg > 1:536645/1‑134 (MQ=255)
gcacagccGGGCGCACCCGCAGCTGACGCGCAACGC‑GGGCAATATCGCGCTGCTGCGCGCAGCGGGCGAGCTGGGCCTGATCGACGCCACGCTGGCACTGGCGGTGGGCGATGCCTACCGGCTGTTCcgcgcgc > 1:476511/7‑134 (MQ=255)
acagccGGGCGCACCCGCAGCTGACGCGCAACGC‑GGGCAATATCGCGCTGCTGCGCGCAGCGGGCGAGCTGGGCCTGATCGACGCCACGCTGGCACTGGCgg < 1:945747/98‑1 (MQ=255)
acagccGGGCGCACCCGCAGCTGACGCGCAACGC‑GGGCAATATCGCGCTGCTGCGCGCAGCGGGCGAGCTGGGCCTGATCGACGCCACGCTGGCACTGGCgg > 2:945747/5‑102 (MQ=255)
cACCCGCAGCTGACGCGCAACGC‑GGGCAATATCGCGCTGCTGCGCGCAGCGGGCGAGCTGGGCCTGATCGACGCCACGCTGGCACTGGCGGTGGGCGATGCCTACCGGCTGTTCCGCGCGCGCCAGCACCAGCt > 1:690779/1‑134 (MQ=255)
cccGCAGCTGACGCGCAACGC‑GGGCAATATCGCGCTGCTGCGCGCAGCGGGCGAGCTGGGCCTGATCGACGCCACGCTGGc > 2:125360/1‑81 (MQ=255)
cccGCAGCTGACGCGCAACGC‑GGGCAATATCGCGCTGCTGCGCGCAGCGGGCGAGCTGGGCCTGATCGACGCCACGCTGGc < 1:125360/81‑1 (MQ=255)
acgcgggCAATATCGCGCTGCTGCGCGCAGCGGGCGAGCTGGGCCTGATCGACGCCACGCTGGCACTGGCGGTGGGCGATGCCTACCGGCTGTTCcgcg > 1:964348/5‑99 (MQ=255)
acgcgggCAATATCGCGCTGCTGCGCGCAGCGGGCGAGCTGGGCCTGATCGACGCCACGCTGGCACTGGCGGTGGGCGATGCCTACCGGCTGTTCcgcg < 2:964348/95‑1 (MQ=255)
gcgggCAATATCGCGCTGCTGCGCGCAGCGGGCGAGCTGGGCCTGATCGACGCCACGCTGGCACTGGCGGTGGGCGATGCCTACCGGCTGTTCCGCGCGCGCCAGCACCAGCTGCGGCTCGAGGGGCAGGCGCa > 2:441832/3‑134 (MQ=255)
gcgggCAATATCGCGCTGCTGCGCGCAGCGGGCGAGCTGGGCCTGATCGACGCCACGCTGGCACTGGCGGTGGGCGATGCCTACCGGCTGTTCCGCGCGCGCCAGCACCAGCTGCGGCTCGAGGGGCAGGCGCa < 2:476511/132‑1 (MQ=255)
gcgcTGCTGCGCGCAGCGGGCGAGCTGGGCCTGATCGACGCCACGCTGGCACTGGCGGTGGGCGATGCCTACCGGCTGTTCCGCGCGCGCCAGCACCAGCTGCGGCTCGAGGGGCAGGCGCATGCGCGCGTcgc < 2:536645/134‑1 (MQ=255)
gcgcTGCTGCGCGCAGCGGGCGAGCTGGGCCTGATCGACGCCACGCTGGCACTGGCGGTGGGCGATGCCTACCGGCTGTTCCGCGCGCGCCAGCACCAGCTGCGGCTCGAGGGGCAGGCGCATGCGCGCGTcgc < 2:754221/134‑1 (MQ=255)
gcgcTGCTGCGCGCAGCGGGCGAGCTGGGCCTGATCGACGCCACGCTGGCACTGGCGGTGGGCGATGCCTACCGGCTGTTCCGCGCGCGCCAGCACCAGCTGCGGCTCGAGGGGCAGGCGCATGCGCGCGTcg < 1:441832/133‑1 (MQ=255)
ctgctgCGCGCAGCGGGCGAGCTGGGCCTGATCGACGCCACGCTGGCACTGGCGGTGGGCGATGCCTACCGGCTGTTCCGCGCGCGcc < 2:1445/88‑1 (MQ=255)
ctgctgCGCGCAGCGGGCGAGCTGGGCCTGATCGACGCCACGCTGGCACTGGCGGTGGGCGATGCCTACCGGCTGTTCCGCGCGCGcc > 1:1445/1‑88 (MQ=255)
gctgcGCGCAGCGGGCGAGCTGGGCCTGATCGACGCCACGCTGGCACTGGCGGTGGGCGATGCCTACCGGCTGTTCCGCGCGCGCCAGCACCAGCTGCGGCTCGAGGGGCAGGCGCATGCGCGCGTCGCGccc > 2:550276/1‑133 (MQ=255)
aTCGACGCCACGCTGGCACTGGCGGTGGGCGATGCCTACCGGCTGTTCCGCGCGCGCCAGCACCAGCTGCGGCTCGAGGGGCAGGCGCATGCGCGCGTCGCGCCCGAGTCGGTGGCGCAGCAGACCGGACAGGt < 2:690779/134‑1 (MQ=255)
||
TCGAATTCACGGGTGCAGTACCTGGTGCTGCTGCACAACCCGGGCGCACCCGCAGCTGACGCGCAACGCGGGGCAATATCGCGCTGCTGCGCGCAGCGGGCGAGCTGGGCCTGATCGACGCACCGCTGGCACTGGCGGTGGGCGATGCCTACCGGCTGTTCCGCGCGCGCCAGCACCAGCTGCGGCTCGAGGGGCAGGCGCATGCGCGCGTCGCGCCCGAGTCGGTGGCGCAGCAGACCGGACAGGT > AM260479/1231105‑1231351
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
BRESEQ :: bam2aln output
GCGGCATGGTCGATATCGAATTCACGGGTGCAGTACCTGGTGCTGCTGCACAACCCGGGCGCACCCGCAGCTGACGCGCAACGCGGGGCAATATCGCGCTGCTGCGCGCAGCGGGCGAGCTGGGCCTGATCGACGCACCGCTGGCACTGGCGGTGGGCGATGCCTACCGGCTGTTCCGCGCGCGCCAGCACCAGCTGCGGCTCGAGGGGCAGGCGCATGCGCGCGTCGCGCCCGAGTCGGTGG > AM260479/1231090‑1231332
|
GCGGCATGGTCGATATCGAATTCAC‑GGTGCAGTACCTGGTGCTGCTGCAC‑AGCCGGGCGCACCCGCAGCTGACGCGCAACGC‑GGGCAATATCGCGCTGCTGCGCGCAGCGGGCGAGCTGGGCCTGATCGACGCCACGCTGGCACTGGCGG > GWNJ‑0478:712:GW2002102894th:2:1106:9037:61153/1‑150 (MQ=60)
gctccgtctcgtgggctcggagatgtgtataagagacaggcacagCCGGGCGCACCCGCAGCTGACGCGCAACGC‑GGGCAATATCGCGCTGCTGCGCGCAGCGGGCGAGCTGGGCCTGATCGACGCCACGCTGGCACTGGCGGTaggcat < GWNJ‑0478:712:GW2002102894th:2:1109:17858:38812/105‑7 (MQ=60)
gatgtgtataagagacagACCTGGTGCTGCTGCAC‑AGCCGGGCGCACCCGCAGCTGACGCGCAACGC‑GGGCAATATCGCGCTGCTGCGCGCAGCGGGCGAGCTGGGCCTGATCGACGCCACGCTGGCACTGGCGGTGGGCGATGCCTACC < GWNJ‑0478:712:GW2002102894th:2:2210:18969:79339/132‑1 (MQ=60)
acGGTGCAGTACCTGGTGCTGCTGCAC‑AGCCGGGCGCACCCGCAGCTGACGCGCAACGC‑GGGCAATATCGCGCTGCTGCGCGCAGCGGGCGAGCTGGGCCTGATCGACGCCACGCTGGCACTGGCGGTGGGCGATGCCTACCGGCTGTTC < GWNJ‑0478:712:GW2002102894th:2:1206:20406:51894/148‑1 (MQ=60)
GTGCAGTACCTGGTGCTGCTGCAC‑AGCCGGGCGCACCCGCAGCTGACGCGCAACGC‑GGGCAATATCGCGCTGCTGCGCGCAGCGGGCGAGCTGGGCCTGATCGACGCCACGCTGGCACTGGCGGTGGGCGATGCCTACCGGCTGTTCCGC > GWNJ‑0478:712:GW2002102894th:2:2101:6963:97154/1‑150 (MQ=60)
GTACCTGGTGCTGCTGCAC‑AGCCGGGCGCACCCGCAGCTGACGCGCAACGC‑GGGCAATATCGCGCTGCTGCGCGCAGCGGGCGAGCTGGGCCTGATCGACGCCACGCTGGCACTGGCGGTGGGCGATGCCTACCGGCTGTTCCGCGCGCG > GWNJ‑0478:712:GW2002102894th:2:1215:16735:47668/1‑150 (MQ=60)
CTGCAC‑AGCCGGGCGCACCCGCAGCTGACGCGCAACGC‑GGGCAATATCGCGCTGCTGCGCGCAGCGGGCGAGCTGGGCCTGATCGACGCCACGCTGGCACTGGCGGTGGGCGATGCCTACCGGCTGTTCCGCGCGCGCCAGCACCAGCTG > GWNJ‑0478:712:GW2002102894th:2:2113:12849:5448/1‑150 (MQ=60)
caagcagaagacggcatacgagatgtagctccgtctcgtgggctcggagatgtgtataagagacagGATCGACGCCACGCTGGCACTGGCGGTGGGCGATGCCTACCGGCTGTTCCGCGCGCGCCAGCACCAGCTGCGGCTCGAGGGGCA < GWNJ‑0478:712:GW2002102894th:2:1212:3345:5380/84‑1 (MQ=60)
CGCAGCTGACGCGCAACGC‑GGGCAATATCGCGCTGCTGCGCGCAGCGGGCGAGCTGGGCCTGATCGACGCCACGCTGGCACTGGCGGTGGGCGATGCCTACCGGCTGTTCCGCGCGCGCCAGCACCAGCctgtctcttatacacatctcc > GWNJ‑0478:712:GW2002102894th:2:2212:18323:86167/1‑129 (MQ=60)
gcGGGCAATATCGCGCTGCTGCGCGCAGCGGGCGAGCTGGGCCTGATCGACGCCACGCTGGCACTGGCGGTGGGCGATGCCTACCGGCTGTTCCGCGCGCGCCAGCACCAGCTGCGGCctgtctcttatacacctctccgagcccacg > GWNJ‑0478:712:GW2002102894th:2:1101:1953:11826/3‑118 (MQ=60)
CGCGCTGCTGCGCGCAGCGGGCGAGCTGGGCCTGATCGACGCCACGCTGGCACTGGCGGTGGGCGATGCCTACCGGCTGTTCCGCGCGCGCCAGCACCAGCTGCGGCTCGAGGGGCAGGCGCATGCGCGCGTCGCGCCCGAGTCGGTGG < GWNJ‑0478:712:GW2002102894th:2:1213:16757:60717/149‑1 (MQ=60)
|
GCGGCATGGTCGATATCGAATTCACGGGTGCAGTACCTGGTGCTGCTGCACAACCCGGGCGCACCCGCAGCTGACGCGCAACGCGGGGCAATATCGCGCTGCTGCGCGCAGCGGGCGAGCTGGGCCTGATCGACGCACCGCTGGCACTGGCGGTGGGCGATGCCTACCGGCTGTTCCGCGCGCGCCAGCACCAGCTGCGGCTCGAGGGGCAGGCGCATGCGCGCGTCGCGCCCGAGTCGGTGG > AM260479/1231090‑1231332
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
