Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F1 I1 R1
|
152 |
24.9 |
2083062 |
91.6% |
1908084 |
109.2 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
AM260479 |
2,274,430 |
C→G |
G565G (GGG→GGC) |
h16_A2092 ← |
Lysophospholipase, contains weak SAM‑dependent methyltransferase domain |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | AM260479 | 2,274,430 | 0 | C | G | 100.0%
| 77.2
/ NA
| 21 | G565G (GGG→GGC) | h16_A2092 | Lysophospholipase, contains weak SAM‑dependent methyltransferase domain |
| Reads supporting (aligned to +/- strand): ref base C (0/0); new base G (11/10); total (11/10) |
GCCTGCAGGCAGCTGCGAATGCGCCCCGGCGCCAACCTCCGTCGGCACCGTGCTCATGCGCCGCGCCTGCGCGCCAGGCTGACGGTGAAGATGCCCCACGGGTCGATACGCTGGGTCACCTTGTCAAACCCAGCGGCCCGCACCAGTTCGTCCATCTCGGCCTGCGAGCGGCGGCGCATCACCCAGGCGGCGCCGGCGCGGTGGCTGGTGAGCGCGCGCGCGATGAATTCCAGCTGCGGATGCCAGGGCTGGCCGG > AM260479/2274302‑2274557
|
gCCTGCAGGCAGCTGCGAATGCGCCCCGGCGCCAACCTCCGTCGGCACCGTGCTCATGCGCCGCGCCTGCGCGCCAGGCTGACGGTGAAGATGCCCCACGGGTCGATACGCTGGGTCACCTTGTCAAAGCCAg < 2:135526/133‑1 (MQ=255)
gAATGCGCCCCGGCGCCAACCTCCGTCGGCACCGTGCTCATGCGCCGCGCCTGCGCGCCAGGCTGACGGTGAAGATGCCCCACGGGTCGATACGCTGGGTCACCTTGTCAAAGCCAGCGGCCCGCACCAGTTCg > 1:378558/1‑134 (MQ=255)
tGCTCATGCGCCGCGCCTGCGCGCCAGGCTGACGGTGAAGATGCCCCACGGGTCGATACGCTGGGTCACCTTGTCAAAGCCAGCGGCCCGCACCAGTTCGTCCATCTCGGCCTGCGAGCGGCGGCGCATCAcc > 1:295986/1‑133 (MQ=255)
tCATGCGCCGCGCCTGCGCGCCAGGCTGACGGTGAAGATGCCCCACGGGTCGATACGCTGGGTCACCTTGTCAAAGCCAGCGGCCCGCACCAGTTCGTCCATCTCGGCCTGCGAGCGGCGGCGCATCACCCAgg > 2:282403/1‑134 (MQ=255)
aTGCGCCGCGCCTGCGCGCCAGGCTGACGGTGAAGATGCCCCACGGGTCGATACGCTGGGTCACCTTGTCAAAGCCAGCGGCCCGCACCAGTTCGTCCATCTCGGCCTGCGAGCGGCGGCGCATCACCCAggcg < 2:295986/134‑1 (MQ=255)
gccTGCGCGCCAGGCTGACGGTGAAGATGCCCCACGGGTCGATACGCTGGGTCACCTTGTCAAAGCCAGCGGCCCGCACCAGTTCGTCCATCTCGGCCTGCGAGCGGCGGCGCATCACCCAGGcggcgccggcg > 1:910726/1‑134 (MQ=255)
ccTGCGCGCCAGGCTGACGGTGAAGATGCCCCACGGGTCGATACGCTGGGTCACCTTGTCAAAGCCAGCGGCCCGCACCAGTTCGTCCATCTCGGCCTGCGAGCGGCGGCGCATCACCCAGGcggcgccg < 2:98567/130‑1 (MQ=255)
ccTGCGCGCCAGGCTGACGGTGAAGATGCCCCACGGGTCGATACGCTGGGTCACCTTGTCAAAGCCAGCGGCCCGCACCAGTTCGTCCATCTCGGCCTGCGAGCGGCGGCGCATCACCCAGGcggcgccg > 1:98567/1‑130 (MQ=255)
tGCGCGCCAGGCTGACGGTGAAGATGCCCCACGGGTCGATACGCTGGGTCACCTTGTCAAAGCCAGCGGCCCGCACCAGTTCGTCCATCTCGGCCTGCGAGCGGCGGCGCATCACCCAGGCGGCGCCGGCGCgg > 1:219194/1‑134 (MQ=255)
tGCGCGCCAGGCTGACGGTGAAGATGCCCCACGGGTCGATACGCTGGGTCACCTTGTCAAAGCCAGCGGCCCGCACCAGTTCGTCCATCTCGGCCTGCGAGCGGCGGCGCATCACCCAGGCGGCGCCGGCGCgg > 2:681847/1‑134 (MQ=255)
tGCCCCACGGGTCGATACGCTGGGTCACCTTGTCAAAGCCAGCGGCCCGCACCAGTTCGTCCATCTCGGCCTGCGAGCGGCGGCGCATCACCCAGGCGGCGCCGGCGCGGTGGCTGGTGAgcgcgcgcgc > 1:407895/1‑130 (MQ=255)
tGCCCCACGGGTCGATACGCTGGGTCACCTTGTCAAAGCCAGCGGCCCGCACCAGTTCGTCCATCTCGGCCTGCGAGCGGCGGCGCATCACCCAGGCGGCGCCGGCGCGGTGGCTGGTGAgcgcgcgcgc < 2:407895/130‑1 (MQ=255)
ccACGGGTCGATACGCTGGGTCACCTTGTCAAAGCCAGCGGCCCGCACCAGTTCGTCCATCTCGGCCTGCGAGCGGCGGCGCATCACCCAGGCGGCGCCGGCGCGGTGGCTGGAGAGCGCGCGCGCGATGAAtt < 1:681847/134‑1 (MQ=255)
ggTCGATACGCTGGGTCACCTTGTCAAAGCCAGCGGCCCGCACCAGTTCGTCCATCTCGGCCTGCGAGCGGCGGCGCATCACCCAGGCGGCGCCGGCGCGGTGGCTGGTGAGCGCGCGCGCGATGAATTCCAGc < 1:372789/134‑1 (MQ=255)
cTGGGTCACCTTGTCAAAGCCAGCGGCCCGCACCAGTTCGTCCATCTCGGCCTGCGAGCGGCGGCGCATCACCCAGGCGGCGCCGGCGCGGTGGCTGGTGAGCGCGCGCGCGATGAATTCCAGCTGCGGATGcc < 2:219194/134‑1 (MQ=255)
cTGGGTCACCTTGTCAAAGCCAGCGGCCCGCACCAGTTCGTCCATCTCGGCCTGCGAGCGGCGGCGCATCACCCAGGCGGCGCCGGCGCGGTGGCTGGTGAGCGCGCGCGCGATGAATTCCAGCTGCGGATGcc < 2:378558/134‑1 (MQ=255)
tCACCTTGTCAAAGCCAGCGGCCCGCACCAGTTCGTCCATCTCgg < 1:324466/45‑1 (MQ=255)
tCACCTTGTCAAAGCCAGCGGCCCGCACCAGTTCGTCCATCTCgg > 2:324466/1‑45 (MQ=255)
tCACCTTGTCAAAGCCAGCGGCCCGCACCAGTTCGTCCATCTCGGCCTGCGAGCGGCGGCGCATCACCCAGGCGGCGCCGGCGCGGTGGCTGGTg > 2:157019/1‑95 (MQ=255)
tCACCTTGTCAAAGCCAGCGGCCCGCACCAGTTCGTCCATCTCGGCCTGCGAGCGGCGGCGCATCACCCAGGCGGCGCCGGCGCGGTGGCTGGTg < 1:157019/95‑1 (MQ=255)
gTCAAAGCCAGCGGCCCGCACCAGTTCGTCCATCTCGGCCTGCGAGCGGCGGCGCATCACCCAGGCGGCGCCGGCGCGGTGGCTGGTGAGCGCGCGCGCGATGAATTCCAGCTGCGGATGCCAGGGCTGGCCgg > 2:928081/1‑134 (MQ=255)
|
GCCTGCAGGCAGCTGCGAATGCGCCCCGGCGCCAACCTCCGTCGGCACCGTGCTCATGCGCCGCGCCTGCGCGCCAGGCTGACGGTGAAGATGCCCCACGGGTCGATACGCTGGGTCACCTTGTCAAACCCAGCGGCCCGCACCAGTTCGTCCATCTCGGCCTGCGAGCGGCGGCGCATCACCCAGGCGGCGCCGGCGCGGTGGCTGGTGAGCGCGCGCGCGATGAATTCCAGCTGCGGATGCCAGGGCTGGCCGG > AM260479/2274302‑2274557
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
BRESEQ :: bam2aln output
CCCCAGGGCCGGTTGCCTGCAGGCAGCTGCGAATGCGCCCCGGCGCCAACCTCCGTCGGCACCGTGCTCATGCGCCGCGCCTGCGCGCCAGGCTGACGGTGAAGATGCCCCACGGGTCGATACGCTGGGTCACCTTGTCAAACCCAGCGGCCCGCACCAGTTCGTCCATCTCGGCCTGCGAGCGGCGGCGCATCACCCAGGCGGCGCCGGCGCGGTGGCTGGTGAGCGCGCGCGCGATGAATTCCAGCTGCGGATGCCAGGGCTGGCCGGTGTAGACCAGATAGC > AM260479/2274288‑2274572
|
catacgagctgtagctccgtctcgtgggctcggagatgtgtataagagacagCCGTCGGCACCGTGCTCATGCGCCGCGCCTGCGCGCCAGGCTGACGGTGAAGATGCCCCACGGGTCGATACGCTGGGTCACCTTGTCAAAGCCAGCGG < GWNJ‑0478:712:GW2002102894th:2:2110:4919:90387/98‑1 (MQ=60)
CCTGCAGGCAGCTGCGAATGCGCCCCGGCGCCAACCTCCGTCGGCACCGTGCTCATGCGCCGCGCCTGCGCGCCAGGCTGACGGTGAAGATGCCCCACGGGTCGATACGCTGGGTCACCTTGTCAAAGCCAGCGGCCCGCACCAGTTCGT > GWNJ‑0478:712:GW2002102894th:2:1208:2170:98963/1‑150 (MQ=60)
ttttttttcaagcagaagacggcatacgagatgtagctccgtctcgtgggctcggagatgtgtataagagacaGGGTCGATACGCTGGGTCACCTTGTCAAAGCCAGCGGCCCGCACCAGTTCGTCCATCTCGGCCTGCGAGCGGCGGC < GWNJ‑0478:712:GW2002102894th:2:1204:18166:59597/76‑1 (MQ=60)
CCTCCGTCGGCACCGTGCTCATGCGCCGCGCCTGCGCGCCAGGCTGACGGTGAAGATGCCCCACGGGTCGATACGCTGGGTCACCTTGTCAAAGCCAGCGGCCCGCACCAGTTCGTCCATCTCGGCCTGCGAGCGGCGGCGCATCACCC > GWNJ‑0478:712:GW2002102894th:2:1202:10949:70070/1‑149 (MQ=60)
GTGCTCATGCGCCGCGCCTGCGCGCCAGGCTGACGGTGAAGATGCCCCACGGGTCGATACGCTGGGTCACCTTGTCAAAGCCAGCGGCCCGCACCAGTTCGTCCATCTCGGCCTGCGAGCGGCGGCGCATCACCCAGGCGGCGCCGGCGC > GWNJ‑0478:712:GW2002102894th:2:2208:8388:75535/1‑150 (MQ=60)
TGCTCATGCGCCGCGCCTGCGCGCCAGGCTGACGGTGAAGATGCCCCACGGGTCGATACGCTGGGTCACCTTGTCAAAGCCAGCGGCCCGCACCAGTTCGTCCATCTCGGCCTGCGAGCGGCGGCGCATCACCCAGGCGGCGCCGGCGCG > GWNJ‑0478:712:GW2002102894th:2:1106:6597:70120/1‑150 (MQ=60)
CTCATGCGCCGCGCCTGCGCGCCAGGCTGACGGTGAAGATGCCCCACGGGTCGATACGCTGGGTCACCTTGTCAAAGCCAGCGGCCCGCACCAGTTCGTCCATCTCGGCCTGCGAGCGGCGGCGCATCACCCAGGCGGCGCCGGCGCGGT > GWNJ‑0478:712:GW2002102894th:2:1115:4444:77678/1‑150 (MQ=60)
CGCCGCGCCTGCGCGCCAGGCTGACGGTGAAGATGCCCCACGGGTCGATACGCTGGGTCACCTTGTCAAAGCCAGCGGCCCGCACCtgtctcttatacacatctccgagcccacgagacggagctacatctcgtatgccgtcttctgctt > GWNJ‑0478:712:GW2002102894th:2:2114:19112:26945/1‑86 (MQ=60)
gctcggagatgtgtataagagacaGGGTCGATACGCTGGGTCACCTTGTCAAAGCCAGCGGCCCGCACCAGTTCGTCCATCTCGGCCTGCGAGCGGCGGCGCATCACCCAGGCGGCGCCGGCGCGGTGGCTGGTGAGCGCGCGCGCGATG < GWNJ‑0478:712:GW2002102894th:2:1110:6320:26463/126‑1 (MQ=60)
GGCTGACGGTGAAGATGCCCCACGGGTCGATACGCTGGGTCACCTTGTCAAAGCCAGCGGCCCGCACCAGTTCGTCCATCTCGGCCTGCGAGCGGCGGCGCATCACCCAGGCGGCGCCGGCGCGGTGGCTGGTGAGCGCGCGCGCGATG > GWNJ‑0478:712:GW2002102894th:2:1211:14461:71720/1‑149 (MQ=60)
CCCACGGGTCGATACGCTGGGTCACCTTGTCAAAGCCAGCGGCCCGCACCAGTTCGTCCATCTCGGCCTGCGAGCGGCGGCGCATCACCCAGGCGGCGCCGGCGCGGTGGCTGGAGAGCGCGCGCGCGATGAATTCCAGCTGCGGATGCC < GWNJ‑0478:712:GW2002102894th:2:2110:3144:52094/150‑1 (MQ=60)
GGGTCGATACGCTGGGTCACCTTGTCAAAGCCAGCGGCCCGCACCAGTTCGTCCATCTCGGCCTGCGAGCGGCGGCGCATCACCCAGGCGGCGCCGGCGCGGTGGCTGGTGAGCGCGCGCGCGATGAATTCCAGCTGCGGATGCCAGGGC < GWNJ‑0478:712:GW2002102894th:2:1208:12028:64979/150‑1 (MQ=60)
GTCAAAGCCAGCGGCCCGCACCAGTTCGTCCATCTCGGCCTGCGAGCGCCGGCGCcgcctgggtctgtctcttatacacatctccgagcccacgagacggagctacatctcgtatgccgtcttctgcttgaaaaaaaaaaaaaaaaaga > GWNJ‑0478:712:GW2002102894th:2:2215:12872:39940/1‑55 (MQ=60)
|
CCCCAGGGCCGGTTGCCTGCAGGCAGCTGCGAATGCGCCCCGGCGCCAACCTCCGTCGGCACCGTGCTCATGCGCCGCGCCTGCGCGCCAGGCTGACGGTGAAGATGCCCCACGGGTCGATACGCTGGGTCACCTTGTCAAACCCAGCGGCCCGCACCAGTTCGTCCATCTCGGCCTGCGAGCGGCGGCGCATCACCCAGGCGGCGCCGGCGCGGTGGCTGGTGAGCGCGCGCGCGATGAATTCCAGCTGCGGATGCCAGGGCTGGCCGGTGTAGACCAGATAGC > AM260479/2274288‑2274572
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
