Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F1 I1 R1
|
152 |
24.9 |
2083062 |
91.6% |
1908084 |
109.2 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
AM260479 |
2,616,505 |
C→G |
A45G (GCC→GGC) |
h16_A2404 → |
predicted esterase of the alpha‑beta hydrolase superfamily |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | AM260479 | 2,616,505 | 0 | C | G | 100.0%
| 68.7
/ NA
| 19 | A45G (GCC→GGC) | h16_A2404 | predicted esterase of the alpha‑beta hydrolase superfamily |
| Reads supporting (aligned to +/- strand): ref base C (0/0); new base G (9/10); total (9/10) |
CGTCATTTCCTCGGTATCTCCGCGGCCGCCCTGCTGGCGGGCTGCTCGTTCGGCCCGCGTCCCGCGCCGCCGCTCGCCACCACCCCGCCCGCGCCGCGGCCCGTCAAGATCGGCCTGGCGCTGGCCGGCGGCGCGGCGCGCGGCTTTGCCCATATCGGCGTGATCAAGGCGCTCGAAGCGCAAGGCATCCATGCCGACCTGGTCACCGGCACCAGCGCCGGCGCGGTCGTGGCGGCGCTTTACGCCAGCG > AM260479/2616381‑2616630
|
cgTCATTTCCTCGGTATCTCCGCGGCCGCCCTGCTGGCGGGCTGCTCGTTCGGCCCGCGTCCCGCGCCGCCGCTCGCCACCACCCCGCCCGCGCCGCGGCCCGTCAAGATCGGCCTGGCGCTGGGCGgcggcgc > 2:554644/1‑134 (MQ=255)
tCATTTCCTCGGTATCTCCGCGGCCGCCCTGCTGGCGGGCTGCTCGTTCGGCCCGCGTCCCGCGCCGCCGCTCGCCACCACCCCGCCCGCGCCGCGGCCCGTCAAGATCGGCCTGGCGCTGGGCGgcggcgcgg < 1:554644/134‑1 (MQ=255)
tCATTTCCTCGGTATCTCCGCGGCCGCCCTGCTGGCGGGCTGCTCGTTCGGCCCGCGTCCCGCGCCGCCGCTCGCCACCACCCCGCCCGCGCCGCGGCCCGACAAGATCGGCCTGGCGCTGGGCGgcggcgcgg < 1:26445/134‑1 (MQ=255)
ccTCGGTATCTCCGCGGCCGCCCTGCTGGCGGGCTGCTCGTTCGGCCCGCGTCCCGCGCCGCCGCTCGCCACCACCCCGCCCGCGCCGCGGCCCGTCAAGATCGGCCTGGCGCTGGGCGGCGgcgcggcgcgc < 1:769771/133‑1 (MQ=255)
cccTGCTGGCGGGCTGCTAGTTCGGCCCGCGTCCCGCGCCGCCGCTCGCCACCACCCCGCCCGCGCCGCGGCCCGTCAAGATCGGCCTGGCGCTGGGCGGCGGCGCGGCGCGCGGCTTTGCCCATATCGGCGt > 2:223057/1‑133 (MQ=255)
cgccgcTCGCCACCACCCCGCCCGCGCCGCGGCCCGTCAAGATCGGCCTGGCGCTGGGCGGCGGCGCGGCGCGCGGCTTTGCCCATATCGGCGTGATCAAGGCGCTCGAAGCGCAAGGCATCCATGCCGACCTg < 1:1001141/134‑1 (MQ=255)
cTCGCCACCACCCCGCCCGCGCCGCGGCCCGTCAAGATCGGCCTGGCGCTGGGCGGCGGCGCGGCGCGCGGCTTTGCCCATATCGGCGTGATCAAGGCGCTCGAAGCGCAAGGCATCCATGCCGACCTGGTCAc > 2:236891/1‑134 (MQ=255)
ccaccCCGCCCGCGCCGCGGCCCGTCAAGATCGGCCTGGCGCTGGGCGGCGGCGCGGCGCGCGGCTTTGCCCATATCGGCGTGATCAAGGCGCTCGAAGCGCAAGGCATCCATGCCGACCTGGTCACCGGCAcc < 1:223057/134‑1 (MQ=255)
ccgcccgcGCCGCGGCCCGTCAAGATCGGCCTGGCGCTGGGCGGCGGCGCGGCGCGCGGCTTTGCCCATATCGGCGTGATCAAGGCGCTCGAAGCGCAAGGCATCCATGCCGACCTGGTCACCGGCa > 1:415285/1‑127 (MQ=255)
ccgcccgcGCCGCGGCCCGTCAAGATCGGCCTGGCGCTGGGCGGCGGCGCGGCGCGCGGCTTTGCCCATATCGGCGTGATCAAGGCGCTCGAAGCGCAAGGCATCCATGCCGACCTGGTCACCGGCa < 2:415285/127‑1 (MQ=255)
gcccgcGCCGCGGCCCGTCAAGATCGGCCTGGCGCTGGGCGGCGGCGCGGCGCGCGGCTTTGCCCATATCGGCGTGATCAAGGCGCTCGAAGCGCAAGGCATc > 1:858528/1‑103 (MQ=255)
gcccgcGCCGCGGCCCGTCAAGATCGGCCTGGCGCTGGGCGGCGGCGCGGCGCGCGGCTTTGCCCATATCGGCGTGATCAAGGCGCTCGAAGCGCAAGGCATc < 2:858528/103‑1 (MQ=255)
cccgcGCCGCGGCCCGTCAAGATCGGCCTGGCGCTGGGCGGCGgcgcggcgcgc < 1:832635/54‑1 (MQ=255)
cccgcGCCGCGGCCCGTCAAGATCGGCCTGGCGCTGGGCGGCGgcgcggcgcgc > 2:832635/1‑54 (MQ=255)
cgccgcgGCCCGTCAAGATCGGCCTGGCGCTGGGCGGCGGCGCGGCGCGCGGCTTTGCCCATATCGGCGTGATCAAGgcg < 1:990534/80‑1 (MQ=255)
cgccgcgGCCCGTCAAGATCGGCCTGGCGCTGGGCGGCGGCGCGGCGCGCGGCTTTGCCCATATCGGCGTGATCAAGgcg > 2:990534/1‑80 (MQ=255)
gCCTGGCGCTGGGCGGCGGCGCGGCGCGCGGCTTTGCCCATATCGGCGTGATCAAGGCGCTCGAAGCGCAAg > 1:724030/1‑72 (MQ=255)
gCCTGGCGCTGGGCGGCGGCGCGGCGCGCGGCTTTGCCCATATCGGCGTGATCAAGGCGCTCGAAGCGCAAg < 2:724030/72‑1 (MQ=255)
gcgcTGGGCGGCGGCGCGGCGCGCGGCTTTGCCCATATCGGCGTGATCAAGGCGCTCGAAGCGCAAGGCATCCATGCCGACCTGGTCACCGGCACCAGCGCCGGCGCGGTCGTGGCGGCGCTTTACGCCAGCg > 1:58458/1‑133 (MQ=255)
|
CGTCATTTCCTCGGTATCTCCGCGGCCGCCCTGCTGGCGGGCTGCTCGTTCGGCCCGCGTCCCGCGCCGCCGCTCGCCACCACCCCGCCCGCGCCGCGGCCCGTCAAGATCGGCCTGGCGCTGGCCGGCGGCGCGGCGCGCGGCTTTGCCCATATCGGCGTGATCAAGGCGCTCGAAGCGCAAGGCATCCATGCCGACCTGGTCACCGGCACCAGCGCCGGCGCGGTCGTGGCGGCGCTTTACGCCAGCG > AM260479/2616381‑2616630
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
BRESEQ :: bam2aln output
GTCATTTCCTCGGTATCTCCGCGGCCGCCCTGCTGGCGGGCTGCTCGTTCGGCCCGCGTCCCGCGCCGCCGCTCGCCACCACCCCGCCCGCGCCGCGGCCCGTCAAGATCGGCCTGGCGCTGGCCGGCGGCGCGGCGCGCGGCTTTGCCCATATCGGCGTGATCAAGGCGCTCGAAGCGCAAGGCATCCATGCCGACCTGGTCACCGGCACCAGCGCCGGCGCGGTCGTGGCGGCGCTTTACGCCAGCGG > AM260479/2616382‑2616631
|
GTCATTTCCTCGGTATCTCCGCGGCCGCCCTGCTGGCGGGCTGCTCGTTCGGCCCGCGTCCCGCGCCGCCGCTCGCCACCACCCCGCCCGCGCCGCGGCCCGTCAAGATCGGCCTGGCGCTGGGCGGCGGCGCGGCGCGCGGCTTTGCCC < GWNJ‑0478:712:GW2002102894th:2:2102:15521:26384/150‑1 (MQ=60)
GTCATTTCCTCGGTATCTCCGCGGCCGCCCTGCTGGCGGGCTGCTCGTTCGGCCCGCGTCCCGCGCCGCCGCTCGCCACCACCCCGCCCGCGCCGCGGCCCGACAAGATCGGCCTGGCGCTGGGCGGCGGCGCGGCGCGCGGCTTTGCCC < GWNJ‑0478:712:GW2002102894th:2:1103:1778:87314/150‑1 (MQ=60)
caagcagaagacggcatacgagatgtagctccgtctcgtgggctcggagatgtgtataagagacaGCTCGCCACCACCCCGCCCGCGCCGCGGCCCGTCAAGATCGGCCTGGCGCTGGGCGGCGGCGCGGCGCGCGGCTTTGCCCATATC < GWNJ‑0478:712:GW2002102894th:2:2204:2293:80069/85‑1 (MQ=60)
TCCTCGGTATCTCCGCGGCCGCCCTGCTGGCGGGCTGCTCGTTCGGCCCGCGTCCCGCGCCGCCGCTCGCCACCACCCCGCCCGCGCCGCGGCCCGTCAAGATCGGCCTGGCGCTGGGCGGCGGCGCGGCGCGCGGCTTTGCCCATATC < GWNJ‑0478:712:GW2002102894th:2:2116:15886:50940/149‑1 (MQ=60)
agctccgtctcgtgggctcggagatgtgtataagagacaGCCACCACCCCGCCCGCGCCGCGGCCCGTCAAGATCGGCCTGGCGCTGGGCGGCGGCGCGGCGCGCGGCTTTGCCCATATCGGCGTGATCAAGGCGCTCGAAGCGCAAGGC < GWNJ‑0478:712:GW2002102894th:2:2214:2206:29545/111‑1 (MQ=60)
CCGCCGCTCGCCACCACCCCGCCCGCGCCGCGGCCCGTCAAGATCGGCCTGGCGCTGGGCGGCGGCGCGGCGCGCGGCTTTGCCCATATCGGCGTGATCAAGGCGCTCGAAGCGCAAGGCATCCATGCCGACCTGGTCACCGGCACCAGC < GWNJ‑0478:712:GW2002102894th:2:2214:13381:86430/150‑1 (MQ=60)
CCGCTCGCCACCACCCCGCCCGCGCCGCGGCCCGTCAAGATCGGCCTGGCGCTGGGCGGCGGCGCGGCGCGCGGCTTTGCCCATATCGGCGTGATCAAGGCGCTCGAAGCGCAAGGCATCCATGCCGACCTGGTCACCGGCACCAGCGCC > GWNJ‑0478:712:GW2002102894th:2:1210:3570:14182/1‑150 (MQ=60)
GCTCGCCACCACCCCGCCCGCGCCGCGGCCCGTCAAGATCGGCCTGGCGCTGGGCGGCGGCGCGGCGCGCGGCTTTGCCCATATCGGCGTGATCAAGGCGCTCGAAGCGCAAGGCATCCATGCCGACCTGGTCctgtctcttatacacat > GWNJ‑0478:712:GW2002102894th:2:2209:3517:48361/1‑133 (MQ=60)
ACCACCCCGCCCGCGCCGCGGCCCGTCAAGATCGGCCTGGCGCTGGGCGGCGGCGCGGCGCGCGGCTTTGCCCATATCGGCGTGATCAAGGCGCTCGAAGCGCAAGGCATCCATGCCGACCTGGTCACCGGCACCAGCGCCGGCGCGGTC < GWNJ‑0478:712:GW2002102894th:2:1115:5190:98917/150‑1 (MQ=60)
GGCCCGTCAAGATCGGCCTGGCGCTGGGCGGCGGCGCGGCGCGCGGCTTTGCCCATATCGGCGTGATCAAGGCGCTCGAAGCGCAAGGCATCCATGCCGACCctgtctcttatacacatctccgagcccacgagacggagctacatctcg > GWNJ‑0478:712:GW2002102894th:2:2112:19697:96175/1‑102 (MQ=60)
GTCAAGATCGGCCTGGCGCTGGGCGGCGGCGCGGCGCGCGGCTTTGCCCATATCGGCGTGATCAAGGCGCTCGAAGCGCAAGGCATCCATGCCGACCTGGTCACCGGCACCAGCGCCGGCGCGGTCGTGGCGGCGCTTTACGCCAGCGG > GWNJ‑0478:712:GW2002102894th:2:1104:14134:7083/1‑149 (MQ=60)
|
GTCATTTCCTCGGTATCTCCGCGGCCGCCCTGCTGGCGGGCTGCTCGTTCGGCCCGCGTCCCGCGCCGCCGCTCGCCACCACCCCGCCCGCGCCGCGGCCCGTCAAGATCGGCCTGGCGCTGGCCGGCGGCGCGGCGCGCGGCTTTGCCCATATCGGCGTGATCAAGGCGCTCGAAGCGCAAGGCATCCATGCCGACCTGGTCACCGGCACCAGCGCCGGCGCGGTCGTGGCGGCGCTTTACGCCAGCGG > AM260479/2616382‑2616631
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
