Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F1 I1 R1
|
152 |
24.9 |
2083062 |
91.6% |
1908084 |
109.2 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
AM260479 |
3,248,092 |
G→C |
intergenic (+43/‑175) |
h16_A3001 → / → hemK1 |
transcriptional regulator, LysR‑family/methylase of polypeptide chain release factors |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | AM260479 | 3,248,092 | 0 | G | C | 100.0%
| 109.0
/ NA
| 29 | intergenic (+43/‑175) | h16_A3001/hemK1 | transcriptional regulator, LysR‑family/methylase of polypeptide chain release factors |
| Reads supporting (aligned to +/- strand): ref base G (0/0); new base C (12/17); total (12/17) |
TCGTCGCCGGCGTCGCCTATGATGCCCGAGCCGCGACCCGCAACCTTGCCCGGCATCTCGGCCGGCCACCTGTTTGGCAGCAACCGTGACGCACCTGGCCGTC‑AGCAGGGTGCCTACCTGCGCAGCTTTGTGTACACCTTCATCGAGCTGTTCTCGCCGACGCTCAACCGCAAGCTGGTTGAGCAGGCCATGTCCGGCGAACACGAAGCCTACGAACTCTGAGGACGGCCCGCCGCCTCTCCCTATATAAAAAGCAAACC > AM260479/3247961‑3248220
|
tcgtcgCCGGCGTCGCCTATGATG‑CCGAG‑CGCGA‑CCGCAACC‑TGCGCGGCATCTCGGCCGTCCACCTGTTTGGCAGCAA‑CGTGACGCACCTGGCCGTCAAGCAGGGTGCCTACCTGCGCAGCTTTGTCTACAcc < 2:980796/134‑1 (MQ=255)
cgaccGCAACC‑TGCGCGGCATCTCGGCCGGCCACCTGTTTGGCAGCAA‑CGTGACGCACCTGGCCGTCAAGCAGGGTGCCTACCTGCGCAGCTTTGTCTACACCTTCATCGAGCTGTTCTCGCCGACGCTCAAcc < 2:248403/131‑1 (MQ=255)
accGCAACC‑TGCGCGGCATCTCGGCCGGCCACCTGTTTGGCAGCAA‑CGTGACGCACCTGGCCGTCAAGCAGGGTGCCTACCTGCGCAGCTTTGTCTACACCTTCATCGAGCTGTTCTCGCCGACGCTCAACCg > 2:72798/2‑133 (MQ=255)
accGCAACC‑TGCGCGGCATCTCGGCCGGCCACCTGTTTGGCAGCAA‑CGTGACGCACCTGGCCGTCAAGCAGGGTGCCTACCTGCGCAGCTTTGTCTACACCTTCATCGAGCTGTTCTCGCCGACGCTCAACCg > 1:747599/2‑133 (MQ=255)
ctgcgcGGCATCTCGGCCGGCCACCTGTTTGGCAGCAA‑CGTGACGCACCTGGCCGTCAAGCAGGGTGCCTACCTGCGCAGCTTTGTCTACACCTTCATCGAGCTGTTCTCGCCGACGCTCAACCGCAAGCTGGt < 1:72798/129‑1 (MQ=255)
gCATCTCGGCCGGCCACCTGTTTGGCAGCAA‑CGTGACGCACCTGGCCGTCAAGCAGGGTGCCTACCTGCGCAGCTTTGTCTACACCTTCATCGAGCTGTTCTCGCCGACGCTCAACCGCAAGCTGGTTGAGCAg > 1:122079/1‑134 (MQ=255)
ctcGGCCGGCCACCTGTTTGGCAGCAA‑CGTGACGCACCTGGCCGTCAAGCAGGGTGCCTACCTGCGCAGCTTTGTCTACACCTTCATCGAGCTGTTCTCGCCGACGCTCAACCGCAAGCTGGTTGAGCAGGCCa > 1:28876/1‑134 (MQ=255)
gccggccACCTGTTTGGCAGCAA‑CGTGACGCACCTGGCCGTCAAGCAGGGTGCCTACCTGCGCAGCTTTGTCTACACCTTCATCGAGCTGTTCTCGCCGACGCTCAACCGCAAGCTGGTTGAGCAGGCCATGTc < 1:773917/134‑1 (MQ=255)
ccggccACCTGTTTGGCAGCAA‑CGTGACGCACCTGGCCGTCAAGCAGGGTGCCTACCTGCGCAGCTTTGTCTACACCTTCATCGAGCTGTTCTCGCCGACGCTCAACCGCAAGCTGGTTGAGCAGGCCATGTcc < 1:78555/134‑1 (MQ=255)
ccTGTTTGGCAGCAA‑CGTGACGCACCTGGCCGTCAAGCAGGGTGCCTACCTGCGCAGCTTTGTCTACACCTTCATCGAGCTGTTCTCGCCGACGCTCAACCGCAAGCTGGTTGAGCAGGCCATGTCCGGCGAac < 2:122079/134‑1 (MQ=255)
ccTGTTTGGCAGCAA‑CGTGACGCACCTGGCCGTCAAGCAGGGTGCCTACCTGCGCAGCTTTGTCTACACCTTCATCGAGCTGTTCTCGCCGACGCTCAACCGCAAGCTGGTTGAGCAGGCCATGTCCGGCGAac < 2:812775/134‑1 (MQ=255)
ccTGTTTAGCAGCAA‑CGTGACGCACCTGGCCGTCAAGCAGGGTGCCTACCTGCGCAGCTTTGTCTACACCTTCATCGAGCTGTTCTCGCCGACGCTCAGTCGCAAGCTGGTTGAGCAGGCCATGTCCGGCTAac < 2:846708/134‑1 (MQ=255)
gcagcaA‑CGTGACGCACCTGGCCGTCAAGCAGGGTGCCTACCTGCGCAGCTTTGTCTACACCTTCATCGAGCTGTTCTCGCCGACGCTCAACCGCAAGCTGGTTGAGCAGGCCATGTCCGGCGAACACGAAGcc < 2:28876/134‑1 (MQ=255)
cGTGACGCACCTGGCCGTCAAGCAGGGTGCCTACCTGCGCAGCTTTGTCTACACCTTCATCGAGCTGTTCTCGCCGACGCTCAAc < 2:178366/85‑1 (MQ=255)
cGTGACGCACCTGGCCGTCAAGCAGGGTGCCTACCTGCGCAGCTTTGTCTACACCTTCATCGAGCTGTTCTCGCCGACGCTCAAc > 1:178366/1‑85 (MQ=255)
cGTGACGCACCTGGCCGTCAAGCAGGGTGCCTACCTGCGCAGCTTTGTCTACACCTTCATCGAGCTGTTCTCGCCGACGCTCAACCGCAAGCTGGTTGAGCAGGCCATGTCCGGCGAACACGAAGcc > 1:80300/1‑127 (MQ=255)
cGTGACGCACCTGGCCGTCAAGCAGGGTGCCTACCTGCGCAGCTTTGTCTACACCTTCATCGAGCTGTTCTCGCCGACGCTCAACCGCAAGCTGGTTGAGCAGGCCATGTCCGGCGAACACGAAGcc < 2:80300/127‑1 (MQ=255)
ccGTCAAGCAGGGTGCCTACCTGCGCAGCTTTGTCTACACCTTCATCGAGCTGTTCTCGCCGACGCTCAACCGCAAGCTGGTTGAGCAGGCCATGTCCGGCGAACACGAAGCCTACGAACTCTGAGGACGGccc > 2:283670/1‑134 (MQ=255)
gtcaagcagGGTGCCTACCTGCGCAGCTTTGTCTACACCTTCATCGAGCTGTTCTCGCCGACGCTCAACCGCAAGCTGGTTGAGCAGGCCATGTCCGGCGAACACGAAGCCTACGAACTCTGAGGACGGCccgc > 2:1014253/5‑134 (MQ=255)
gtcaagcagGGTGCCTACCTGCGCAGCTTTGTCTACACCTTCATCGAGCTGTTCTCGCCGACGCTCAACCGCAAGCTGGTTGAGCAGGCCATGTCCGGCGAACACGAAGCCTACGAACTCTGAGGACGGCccgc < 1:283670/130‑1 (MQ=255)
aagcagGGTGCCTACCTGCGCAGCTTTGTCTACACCTTCATCGAGCTGTTCTCGCCGACGCTCAACCGCAAGCTGGTTGAGCAGGCCATGTCCGGCGAACACGAAGCCTACGAACTCTGAGGACGGCCcgccgc < 1:1014253/133‑1 (MQ=255)
aagcagGGTGCCTACCTGCGCAGCTTTGTCTACACCTTCATCGAGCTGTTCTCGCCGACGCTCAACCGCAAGCTGGTTGAGCAGGCCATGTCCGGCGAACACGAAGCCTACGAACTCTGAGGACGGCCcgccgc < 2:747599/133‑1 (MQ=255)
cagGGTGCCTACCTGCGCAGCTTTGTCTACACCTTCATCGAGCTGTTCTCGCCGACGCTCAACCGCAAGCTGGTTGAGCAGGCCATGTCCGGCGAACACGAAGCCTACGAACTCTGAGGACGGCCCGCCGCctc < 2:36601/134‑1 (MQ=255)
tACCTGCGCAGCTTTGTCTACACCTTCATCGAGCTGTTCTCGCCGACGCTCAACCGCAAGCTGGTTGAGCAGGCCATGTCCGGCGAACACGAAGCCTACGAACTCTGAGGACGGCCCGCCGCCTCTCCCtata > 2:712151/1‑133 (MQ=255)
cAGCTTTGTCTACACCTTCATCGAGCTGTTCTCGCCGACGCTCAACCGCAAGCTGGTTGAGCAGGCCATGTCCGGCGAACACGAAGcc > 1:847115/1‑88 (MQ=255)
cAGCTTTGTCTACACCTTCATCGAGCTGTTCTCGCCGACGCTCAACCGCAAGCTGGTTGAGCAGGCCATGTCCGGCGAACACGAAGcc < 2:847115/88‑1 (MQ=255)
tttGTCTACACCTTCATCGAGCTGTTCTCGCCGACGCTCAACCGCAAGCTGGTTGAGCAGGCCATGTCCGGCGAACACGAAGCCTACGAACTCTGAGGACGGCCCGCCGCCTCTCCCTATATaa < 1:854937/124‑1 (MQ=255)
tttGTCTACACCTTCATCGAGCTGTTCTCGCCGACGCTCAACCGCAAGCTGGTTGAGCAGGCCATGTCCGGCGAACACGAAGCCTACGAACTCTGAGGACGGCCCGCCGCCTCTCCCTATATaa > 2:854937/1‑124 (MQ=255)
tttGTCTACACCTTCATCGAGCTGTTCTCGCCGACGCTCAACCGCAAGCTGGTTGAGCAGGCCATGTCCGGCGAACACGAAGCCTACGAACTCTGAGGACGGCCCGCCGCCTCTCCCTATATAAAAAGCAAAcc > 1:1019906/1‑134 (MQ=255)
|
TCGTCGCCGGCGTCGCCTATGATGCCCGAGCCGCGACCCGCAACCTTGCCCGGCATCTCGGCCGGCCACCTGTTTGGCAGCAACCGTGACGCACCTGGCCGTC‑AGCAGGGTGCCTACCTGCGCAGCTTTGTGTACACCTTCATCGAGCTGTTCTCGCCGACGCTCAACCGCAAGCTGGTTGAGCAGGCCATGTCCGGCGAACACGAAGCCTACGAACTCTGAGGACGGCCCGCCGCCTCTCCCTATATAAAAAGCAAACC > AM260479/3247961‑3248220
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
BRESEQ :: bam2aln output
GAGATCGGGCTGGGCGTGGGATTCGTCGCCGGCGTCGCCTATGATGCCCGAGCCGCGACCCGCAACCTTGCCCGGCATCTCGGCCGGCCACCTGTTTGGCAGCAACCGTGACGCACCTGGCCGTC‑AGCAGGGTGCCTACCTGCGCAGCTTTGTGTACACCTTCATCGAGCTGTTCTCGCCGACGCTCAACCGCAAGCTGGTTGAGCAGGCCATGTCCGGCGAACACGAAGCCTACGAACTCTGAGGACGGCCCGCCGCCTCTCCCTATATAAAAAGCAAACCCAACCATGCATCTGCTGCC > AM260479/3247939‑3248239
|
GAGATCGGGCTGGGCGTGGGCATCGTCGCCGGCGTCGCCTATGATG‑CCGAG‑CGCGA‑CCGCAACC‑TGCGCGGCATCTCGGCCGGCCACCTGTTTGGCAGCAA‑CGTGACGCACCTGGCCGTCAAGCAGGGTGCCTACCTGCGCAGCTTTGTC < GWNJ‑0478:712:GW2002102894th:2:1210:9864:9194/150‑1 (MQ=60)
AGATCGGGCTGGGCGTGGGCATCGTCGCCGGCGTCGCCTATGATG‑CCGAG‑CGCGA‑CCGCAACC‑TGCGCGGCATCTCGGCCGGCCACCTGTTTGGCAGCAA‑CGTGACGCACCTGGCTGTCAAGCAGGGTGCCTACCTGCGCAGCTTTGTCT < GWNJ‑0478:712:GW2002102894th:2:2103:17909:39301/150‑1 (MQ=60)
GCGTGGGCATCGTCGCCGGCGTCGCCTATGATG‑CCGAG‑CGCGA‑CCGCAACC‑TGCGCGGCATCTCGGCCGGCCACCTGTTTGGCAGCAA‑CGTGACGCACCTGGCCGTCAAGCAGGGTGCCTACCTGCGCAGCTTTGTCTACACCTTCATCG < GWNJ‑0478:712:GW2002102894th:2:1208:17714:66442/150‑1 (MQ=60)
GCGTGGGCATCGTCGCCGGCGTCGCCTATGATG‑CCGAG‑CGCGA‑CCGCAACC‑TGCGCGGCATCTCGGCCGGCCACCTGTTTGGCAGCAA‑CGTGACGCACCTGGCCGTCAAGCAGGGTGCCTACCTGCGCAGCTTTGTCTACACCTTCATCG < GWNJ‑0478:712:GW2002102894th:2:1203:11249:81056/150‑1 (MQ=60)
gctgtttttttacaatatctgtggttttttttttttttcaagcagaagacggcatacgagatgtagctccgtctcgtgggctcggagatgtgtataagagacagGCTTTGTCTACACCTTCATCGAGCTGTTCTCGCCGACGCTCAACC < GWNJ‑0478:712:GW2002102894th:2:2212:12025:7369/45‑1 (MQ=60)
atgatgccgagcgcgaccgcaacctgcgCGGCATCTCGGCCGGCCACCTGTTTGGCAGCAA‑CGTGACGCACCTGGCCGTCAAGCAGGGTGCCTACCTGCGCAGCTTTGTCTACACCTTCATCGAGCTGTTCTCGCCGACGCTCAACCGC > GWNJ‑0478:712:GW2002102894th:2:2114:1432:25644/29‑149 (MQ=60)
ACCGCAACC‑TGCGCGGCATCTCGGCCGGCCACCTGTTTGGCAGCAA‑CGTGACGCACCTGGCCGTCAAGCAGGGTGCCTACCTGCGCAGCTTTGTCTACACCTTCATCGAGCTGTTCTCGCCGACGCTCAACCGCAAGCTGGTTGAGCAGG > GWNJ‑0478:712:GW2002102894th:2:1109:9250:18843/1‑150 (MQ=60)
CAACC‑TGCGCGGCATCTCGGCCGGCCACCTGTTTGGCAGCAA‑CGTGACGCACCTGGCCGTCAAGCAGGGTGCCTACCTGCGCAGCTTTGTCTACACCTTCATCGAGCTGTTCTCGCCGACGCTCAACCGCAAGCTGGTTGAGCAGGCCAT > GWNJ‑0478:712:GW2002102894th:2:1105:8988:4293/1‑150 (MQ=60)
CCTGCGCGGCATCTCGGCCGGCCACCTGTTTGGCAGCAA‑CGTGACGCACCTGGCCGTCAAGCAGGGTGCCTACCTGCGCAGCTTTGTCTACACCTTCATCGAGCTGTTCTCGCCGACGCTCAACCGCAAGCTGGTTGAGCAGGCCATGTC < GWNJ‑0478:712:GW2002102894th:2:1107:7694:5308/150‑1 (MQ=60)
GGCCGGCCACCTGTTTGGCAGCAA‑CGTGACGCACCTGGCCGTCAAGCAGGGTGCCTACCTGCGCAGCTTTGTCTACACCTTCATCGAGCTGTTCTCGCCGACGCTCAACCGCAAGCTGGTTGAGCAGGCCATGTCCGGCGAACACGAAGC < GWNJ‑0478:712:GW2002102894th:2:2116:9741:74325/150‑1 (MQ=60)
GCCGGCCACCTGTTTGGCAGCAA‑CGTGACGCACCTGGCCGTCAAGCAGGGTGCCTACCTGCGCAGCTTTGTCTACACCTTCATCGAGCTGTTCTCGCCGACGCTCAACCGCAAGCTGGTTGAGCAGGCCATGTCCGGCGAACACGAAGCC < GWNJ‑0478:712:GW2002102894th:2:1107:11700:42001/150‑1 (MQ=60)
CCTGTTTGGCAGCAA‑CGTGACGCACCTGGCCGTCAAGCAGGGTGCCTACCTGCGCAGCTTTGTCTACACCTTCATCGAGCTGTTCTCGCCGACGCTCAACCGCAAGCTGGTTGAGCtgtctcttatacacatctccgagcccacgagacg > GWNJ‑0478:712:GW2002102894th:2:1113:1518:50514/1‑116 (MQ=60)
CCTGTTTGGCAGCAA‑CGTGACGCACCTGGCCGTCAAGCAGGGTGCCTACCTGCGCAGCTTTGTCTACACCTTCATCGAGCTGTTCTCGCCGACGCTCAACCGCAAGCTGGTTGAGCAGGCCATGTCCGGCGAACACGAAGCCTACGAACT > GWNJ‑0478:712:GW2002102894th:2:1107:16746:52820/1‑150 (MQ=60)
ggctcggagatgtgtataagagacagGCGCAGCTTTGTCTACACCTTCATCGAGCTGTTCTCGCCGACGCTCAACCGCAAGCTGGTTGAGCAGGCCATGTCCGGCGAACACGAAGCCTACGAACTCTGAGGACGGCCCGCCGCCTCTCCC < GWNJ‑0478:712:GW2002102894th:2:2204:6839:95225/124‑1 (MQ=60)
CGTCAAGCAGGGTGCCTACCTGCGCAGCTTTGTCTACACCTTCATCGAGCTGTTCTCGCCGACGCTCAACCGCAAGCTGGTTGAGCAGGCCATGTCCGGCGAACACGAAGCCTACGAACTCTGAGGACGGCCCGCCGCCTCTCCCTATAT < GWNJ‑0478:712:GW2002102894th:2:1203:5636:85243/150‑1 (MQ=60)
caAGCAGGGTGCCTACCTGCGCAGCTTTGTCTACACCTTCATCGAGCTGTTCTCGCCGACGCTCAACCGCAAGCTGGTTGAGCAGGCCATGTCCGGCGAACACGAAGCCTACGAACTCTGAGGACGGCCCGCCGCCTCTCCCTATATAAA < GWNJ‑0478:712:GW2002102894th:2:2215:6500:54658/148‑1 (MQ=60)
GGGTGCCTACCTGCGCAGCTTTGTCTACACCTTCATCGAGCTGTTCTCGCCGACGCTCAACCGCAAGCTGGTTGAGCAGGCCATGTCCGGCGAACACGAAGCCTACGAACTCTGAGGACtgtctcttatacacatctccgagcccacg > GWNJ‑0478:712:GW2002102894th:2:2205:17523:75939/1‑119 (MQ=60)
GCCTACCTGCGCAGCTTTGTCTACACCTTCATCGAGCTGTTCTCGCCGACGCTCAACCGCAAGCTGGTTGAGCAGGCCATGTCCGGCGAACACGAAGCCTACGAACTCTGAGGACGGCCCGCCGCCTCTCCCTATATAAAAAGCAAACCC > GWNJ‑0478:712:GW2002102894th:2:2215:4874:85141/1‑150 (MQ=60)
TACCTGCGCAGCTTTGTCTACACCTTCATCGAGCTGTTCTCGCCGACGCTCAACCGCAAGCTGGTTGAGCAGGCCATGTCCGGCGAACACGAAGCCTACGAACTCTGAGGACGGCCCGCCctgtctcttatacacatctccgagcccacg > GWNJ‑0478:712:GW2002102894th:2:1103:11687:81800/1‑120 (MQ=60)
ACCTGCGCAGCTTTGTCTACACCTTCATCGAGCTGTTCTCGCCGACGCTCAACCGCAAGCTGGTTGAGCAGGCCATGTCCGGCGAACACGAAGCCTACGAACTCTGAGGACGGCCCGCCGCCTCTCCCTATATAAAAAGCAAACCCAACC < GWNJ‑0478:712:GW2002102894th:2:2209:7332:26281/150‑1 (MQ=60)
GCGCAGCTTTGTCTACACCTTCATCGAGCTGTTCTCGCCGACGCTCAACCGCAAGCTGGTTGAGCAGGCCATGTCCGGCGAACACGAAGCCTACGAACTCTGAGGACGGCCCGCCGCCTCTCCCTATATAAAAAGCAAACCCAACCATGC > GWNJ‑0478:712:GW2002102894th:2:1210:11804:91856/1‑150 (MQ=60)
CAGCTTTGTCTACACCTTCATCGAGCTGTTCTCGCCGACGCTCAACCGCAAGCTGGTTGAGCAGGCCATGTCCGGCGAACACGAAGCCTACGAACTCTGAGGACGGCCCGCCGCCTCTCCCTATATAAAAAGCAAACCCAACCATGCATC < GWNJ‑0478:712:GW2002102894th:2:1107:21092:60795/150‑1 (MQ=60)
GTCTACACCTTCATCGAGCTGTTCTCGCCGACGCTCAACCGCAAGCTGGTTGAGCAGGCCATGTCCGGCGAACACGAAGCCTACGAACTCTGAGGACGGCCCGCCGCCctgtctcttatacacatctccgagcccacgagacggagctac > GWNJ‑0478:712:GW2002102894th:2:1113:19059:24105/1‑108 (MQ=60)
|
GAGATCGGGCTGGGCGTGGGATTCGTCGCCGGCGTCGCCTATGATGCCCGAGCCGCGACCCGCAACCTTGCCCGGCATCTCGGCCGGCCACCTGTTTGGCAGCAACCGTGACGCACCTGGCCGTC‑AGCAGGGTGCCTACCTGCGCAGCTTTGTGTACACCTTCATCGAGCTGTTCTCGCCGACGCTCAACCGCAAGCTGGTTGAGCAGGCCATGTCCGGCGAACACGAAGCCTACGAACTCTGAGGACGGCCCGCCGCCTCTCCCTATATAAAAAGCAAACCCAACCATGCATCTGCTGCC > AM260479/3247939‑3248239
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
