Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F1 I1 R1
|
152 |
24.9 |
2083062 |
91.6% |
1908084 |
109.2 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
AM260479 |
3,333,763 |
+G |
coding (5/615 nt) |
serB2 → |
phosphoserine phosphatase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | AM260479 | 3,333,760 | 1 | . | G | 100.0%
| 74.1
/ NA
| 20 | M1R (ATG→AGG) † | serB2 | phosphoserine phosphatase |
| Reads supporting (aligned to +/- strand): ref base . (0/0); new base G (10/10); total (10/10) |
CCAATGCGGCCCCGGCGCCTGCCTGCCTGCCCGCCCGACCTCTCGCCTTAAGGTAAAATCGCCGCCCATGAATCTGGCACTCTTTGACCTCGACCACACCCTGATCCCGACCGACAGCGACCATGAAT‑GGGCCGCTTCCTGGTCCGCCTGGGCGTCGTCGACGAGGAAACCTACCGGCAGAAGAACGACGAGTTCTACGGCCACTACAAAGCCGGCACCCTCGATATCCAGGCCTTCCTGCGCTTTGCGCTGGCGC > AM260479/3333633‑3333888
|
ccAATGCGGCCCCGGCGCCTGCCTGCCTGCCCGCCCGACCTCTCGCCTTAAGGTAAAATCGCCGCCCATGAATCTGGCACTCTTTGACCTCGACCACACCCTGATCCCGACCGACAGCGACCATGAAT‑ggggcc > 1:109997/1‑131 (MQ=255)
cGGCCCCGGCGCCTGCCTGCCTGCCCGCCCGACCTCTCGCCTTAAGGTAAAATCGCCGCCCATGAATCTGGCACTCTTTGACCTCGACCACACCCTGATCCCGACCGACAGCGACCATGAATGGGGCCGCTTcc < 1:748610/134‑1 (MQ=255)
cGGCCCCGGCGCCTGCCTGCCTGCCCGCCCGACCTCTCGCCTTAAGGTAAAATCGCCGCCCATGAATCTGGCACTCTTTGACCTCGACCACACCCTGATCCCGACCGACAGCGACCATGAATGGGGCCGCTTc < 2:46787/133‑1 (MQ=255)
gcctgcctgcctGCCCGCCCGACCTCTCGCCTTAAGGTAAAATCGCCGCCCATGAATCTGGCACTCTTTGACCTCGACCACACCCTGATCCCGACCGACAGCGACCATGAATGGGGCCGCTTcc > 2:550945/1‑124 (MQ=255)
gcctgcctgcctGCCCGCCCGACCTCTCGCCTTAAGGTAAAATCGCCGCCCATGAATCTGGCACTCTTTGACCTCGACCACACCCTGATCCCGACCGACAGCGACCATGAATGGGGCCGCTTcc < 1:43928/124‑1 (MQ=255)
gcctgcctgcctGCCCGCCCGACCTCTCGCCTTAAGGTAAAATCGCCGCCCATGAATCTGGCACTCTTTGACCTCGACCACACCCTGATCCCGACCGACAGCGACCATGAATGGGGCCGCTTcc < 1:550945/124‑1 (MQ=255)
gcctgcctgcctGCCCGCCCGACCTCTCGCCTTAAGGTAAAATCGCCGCCCATGAATCTGGCACTCTTTGACCTCGACCACACCCTGATCCCGACCGACAGCGACCATGAATGGGGCCGCTTcc > 2:43928/1‑124 (MQ=255)
ccgcccgACCTCTCGCCTTAAGGTAAAATCGCCGCCCATGAATCTGGCACTCTTTGACCTCGACCACACCCTGATCCCGACCGACAGCGACCATGAATGGGGCCGCTTCCTGGTCCGCCTGGGCGTCGTcgacg < 1:39244/134‑1 (MQ=255)
ccgcccgACCTCTCGCCTTAAGGTAAAATCGCCGCCCATGAATCTGGCACTCTTTGACCTCGACCACACCCTGATCCCGACCGACAGCGACCATGAATGGGGCCGCTTCCTGGTCCGCCTGGGCGTCGTcgac > 1:353841/1‑133 (MQ=255)
gccgccCATGAATCTGGCACTCTTTGACCTCGACCACACCCTGATCCCGACCGACAGCGACCATGAATGGGGCCGCTTCCTGGTCCGCCTGGGCGTCGTCGACGAGGAAACCTACCGGCAGAAGAACGACGAg < 2:353841/133‑1 (MQ=255)
ccATGAATCTGGCACTCTTTGACCTCGACCACACCCTGATCCCGACCGACAGCGACCATGAATGGGGCCGCTTCCTGGTCCGCCTGGGCGTCGTCGACGAGGAAACCTACCGGCAgaagaa > 1:966221/1‑121 (MQ=255)
ccATGAATCTGGCACTCTTTGACCTCGACCACACCCTGATCCCGACCGACAGCGACCATGAATGGGGCCGCTTCCTGGTCCGCCTGGGCGTCGTCGACGAGGAAACCTACCGGCAgaagaa < 2:966221/121‑1 (MQ=255)
cacaCCCTGATCCCGACCGACAGCGACCATGAATGGGGCCGCTTCCTGGTCCGCCTGGGCGTCGTCGACGAGGAAACCTACCGGCAGAAGAACGACGAGTTCTACGGCCACTACAAAGCCGGCACCCTCGatat > 1:727737/1‑134 (MQ=255)
cacCCTGATCCCGACCGACAGCGACCATGAATGGGGCCGCTTCCTGGTCCGCCTGGGCGTCGTCGACGAGGAAACCTACCGGCAGAAGAACGACGAGTTCTACGGCCACTACAAAGCCGGCACCCTCGATATc > 1:23566/1‑133 (MQ=255)
cgaccgacAGCGACCATGAATGGGGCCGCTTCCTGGTCCGCCTGGGCGTCGTCGACGAGGAAACCTACCGGCAGAAGAACGACGAGTTCTACGGCCACTACAAAGCCGGCACCCTCGATATCCAGGCCTTCCTg > 2:354172/1‑134 (MQ=255)
cgaccgacAGCGACCATGAATGGGGCCGCTTCCTGGTCCGCCTGGGCGTCGTCGACGAGGAAACCTACCGGCAGAAGAACGACGAGTTCTACGGCCACTACAAAGCCGGCACCCTCGATATCCAGGCCTTCCTg > 2:44582/1‑134 (MQ=255)
acAGCGACCATGAATGGGGCCGCTTCCTGGTCCGCCTGGGCGTCGTCGACGAGGAAACCTACCGGCAGAAGAACGACGAGTTCTa > 2:387668/1‑85 (MQ=255)
acAGCGACCATGAATGGGGCCGCTTCCTGGTCCGCCTGGGCGTCGTCGACGAGGAAACCTACCGGCAGAAGAACGACGAGTTCTa > 2:467560/1‑85 (MQ=255)
acAGCGACCATGAATGGGGCCGCTTCCTGGTCCGCCTGGGCGTCGTCGACGAGGAAACCTACCGGCAGAAGAACGACGAGTTCTa < 1:467560/85‑1 (MQ=255)
acAGCGACCATGAATGGGGCCGCTTCCTGGTCCGCCTGGGCGTCGTCGACGAGGAAACCTACCGGCAGAAGAACGACGAGTTCTa < 1:387668/85‑1 (MQ=255)
tGAATGGGGCCGCTTCCTGGTCCGCCTGGGCGTCGTCGACGAGGAAACCTACCGGCAGAAGAACGACGAGTTCTACGGCCACTACAAAGCCGGCACCCTCGATATCCAGGCCTTCCTGCGCTTTGCGCTGgcgc < 2:109997/134‑1 (MQ=255)
|
CCAATGCGGCCCCGGCGCCTGCCTGCCTGCCCGCCCGACCTCTCGCCTTAAGGTAAAATCGCCGCCCATGAATCTGGCACTCTTTGACCTCGACCACACCCTGATCCCGACCGACAGCGACCATGAAT‑GGGCCGCTTCCTGGTCCGCCTGGGCGTCGTCGACGAGGAAACCTACCGGCAGAAGAACGACGAGTTCTACGGCCACTACAAAGCCGGCACCCTCGATATCCAGGCCTTCCTGCGCTTTGCGCTGGCGC > AM260479/3333633‑3333888
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
BRESEQ :: bam2aln output
GCGCCCATACCGCACCCAATGCGGCCCCGGCGCCTGCCTGCCTGCCCGCCCGACCTCTCGCCTTAAGGTAAAATCGCCGCCCATGAATCTGGCACTCTTTGACCTCGACCACACCCTGATCCCGACCGACAGCGACCATGAAT‑GGGCCGCTTCCTGGTCCGCCTGGGCGTCGTCGACGAGGAAACCTACCGGCAGAAGAACGACGAGTTCTACGGCCACTACAAAGCCGGCACCCTCGATATCCAGGCCTTCCTGCGCTTTGCGCTGGCGCCGCTGGCGGCCAACCCGCGCGACC > AM260479/3333618‑3333912
|
GCGCCCATACCGCACCCAATGCGGCCCCGGCGCCTGCCTGCCTGCCCGCCCGACCTCTCGCCTTAAGGTAAAATCGCCGCCCATGAATCTGGCACTCTTTGACCTCGACCACACCCTGATCCCGACCGACAGCGACCATGAATGGGGCCG > GWNJ‑0478:712:GW2002102894th:2:1108:1914:43646/1‑150 (MQ=60)
ACCGCACCCAATGCGGCCCCGGCGCCTGCCTGCCTGCCCGCCCGACCTCTCGCCTTAAGGTAAAATCGCCGCCCATGAATCTGGCACTCTTTGACCTCGACCACACCCTGATCCCGACCGACAGCGACCATGAAT‑GGGgcctgtctctt > GWNJ‑0478:712:GW2002102894th:2:2205:16032:32733/1‑138 (MQ=60)
gtttgttagctttcttttgtttttttttttcaagcagaagacggcatacgagatgtagctccgtctcgtgggctcggagatgtgtataagagacaGACCACACCCTGATCCCGACCGACAGCGACCATGAATGGGGCCGCTTCCTGGTC < GWNJ‑0478:712:GW2002102894th:2:2216:18311:69410/54‑1 (MQ=60)
GCGGCCCCGGCGCCTGCCTGCCTGCCCGCCCGACCTCTCGCCTTAAGGTAAAATCGCCGCCCATGAATCTGGCACTCTTTGACCTCGACCACACCCTGATCCCGACCGACAGCGACCATGAATGGGGCCGCTTCCTGGTCCGCCTGGGCG < GWNJ‑0478:712:GW2002102894th:2:2114:12468:31109/150‑1 (MQ=60)
GCGGCCCCGGCGCCTGCCTGCCTGCCCGCCCGACCTCTCGCCTTAAGGTAAAATCGCCGCCCATGAATCTGGCACTCTTTGACCTCGACCACACCCTGATCCCGACCGACAGCGACCATGAATGGGGCCGCTTCCTGGTCCGCCTGGGCG < GWNJ‑0478:712:GW2002102894th:2:1102:17079:3675/150‑1 (MQ=60)
GCGGCCCCGGCGCCTGCCTGCCTGCCCGCCCGACCTCTCGCCTTAAGGTAAAATCGCCGCCCATGAATCTGGCACTCTTTGACCTCGACCACACCCTGATCCCGACCGACAGCGACCATGAATGGGGCCGCTTCCTGGTCCGCCTGGGCG < GWNJ‑0478:712:GW2002102894th:2:2103:16509:97495/150‑1 (MQ=60)
CGCCTGCCTGCCTGCCCGCCCGACCTCTCGCCTTAAGGTAAAATCGCCGCCCATGAATCTGGCACTCTTTGACCTCGACCACACCCTGATCCCGACCGACAGCGACCATGAATGGGGCCGCTTCCTGGTCCGCCTGGGCGTCGTCGACG > GWNJ‑0478:712:GW2002102894th:2:1206:19775:48530/1‑149 (MQ=60)
CCCGCCCGACCTCTCGCCTTAAGGTAAAATCGCCGCCCATGAATCTGGCACTCTTTGACCTCGACCACACCCTGATCCCGACCGACAGCGACCATGAATGGGGCCGCTTCCTGGTCCGCCTGGGCGTCGTCGACGAGGAAACCTACCGGC < GWNJ‑0478:712:GW2002102894th:2:1105:9391:72399/150‑1 (MQ=60)
AGGTAAAATCGCCGCCCATGAATCTGGCACTCTTTGACCTCGACCACACCCTGATCCCGACCGACAGCGACCATGAATGGGGCCGCTTCCTGGTCCGCCTGGGCGTCGTCGACGAGGAAACCTACCGGCAGAAGAACGACGAGTTCTACG > GWNJ‑0478:712:GW2002102894th:2:2212:15671:96999/1‑150 (MQ=60)
cgtctcgtgggctcggagatgtgtataagagacagCCCTGATCCCGACCGACAGCGACCATGAATGGGGCCGCTTCCTGGTCCGCCTGGGCGTCGTCGACGAGGAAACCTACCGGCAGAAGAACGACGAGTTCTACGGCCACTACAAAGC < GWNJ‑0478:712:GW2002102894th:2:1215:19470:2046/115‑1 (MQ=60)
cgtctcgtgggctcggagatgtgtataagagacagCCCTGATCCCGACCGACAGCGACCATGAATGGGGCCGCTTCCTGGTCCGCCTGGGCGTCGTCGACGAGGAAACCTACCGGCAGAAGAACGACGAGTTCTACGGCCACTACAAAGC < GWNJ‑0478:712:GW2002102894th:2:1209:9962:56818/115‑1 (MQ=60)
CTCTTTGACCTCGACCACACCCTGATCCCGACCGACAGCGACCATGAATGGGGCCGCTTCCTGGTCCGCCTGGGCGTCGTCGACGAGGAAACCTACCGGCAGAAGAACGACGAGTTCTACGGCCACTACAAAGCCGGCACCCTCGATATC > GWNJ‑0478:712:GW2002102894th:2:2115:20368:17829/1‑150 (MQ=60)
CTTTGACCTCGACCACACCCTGATCCCGACCGACAGCGACCATGAATGGGGCCGCTTCCTGGTCCGCCTGGGCGTCGTCGACGAGGAAACCTACCGGCAGAAGAACGACGAGTTCTACGGCCACTACAAAGCCGGCACCCTCGATATCC > GWNJ‑0478:712:GW2002102894th:2:1103:10072:68324/1‑149 (MQ=60)
ACCATGAATGGGGCCGCTTCCTGGTCCGCCTGGGCGTCGTCGACGAGGAAACCTACCGGCAGAAGAACGACGAGTTCTACGGCCACTACAAAGCCGGCACCCTCGATATCCAGGCCTTCCTGCGCTTTGCGCTGGCGCCGCTGGCGGCC > GWNJ‑0478:712:GW2002102894th:2:1212:17187:32199/1‑149 (MQ=60)
ATGAATGGGGCCGCTTCCTGGTCCGCCTGGGCGTCGTCGACGAGGAAACCTACCGGCAGAAGAACGACGAGTTCTACGGCCACTACAAAGCCGGCACCCTCGATATCCAGGCCTTCCTGCGCTTTGCGCTGGCGCCGCTGGCGGCCAACC < GWNJ‑0478:712:GW2002102894th:2:2208:5350:12698/150‑1 (MQ=60)
GCCGCTTCCTGGTCCGCCTGGGCGTCGTCGACGAGGAAACCTACCGGCAGAAGAACGACGAGTTCTACGGCCACTACAAAGCCGGCACCCTCGATATCCAGGCCTTCCTGCGCTTTGCGCTGGCGCCGCTGGCGGCCAACCCGCGCGACC > GWNJ‑0478:712:GW2002102894th:2:2110:5968:95729/1‑150 (MQ=60)
GCCGCTTCCTGGTCCGCCTGGGCGTCGTCGACGAGGAAACCTACCGGCAGAAGAACGACGAGTTCTACGGCCACTACAAAGCCGGCACCCTCGATATCCAGGCCTTCCTGCGCTTTGCGCTGGCGCCGCTGGCGGCCAACCCGCGCGACC < GWNJ‑0478:712:GW2002102894th:2:1116:2540:35220/150‑1 (MQ=60)
|
GCGCCCATACCGCACCCAATGCGGCCCCGGCGCCTGCCTGCCTGCCCGCCCGACCTCTCGCCTTAAGGTAAAATCGCCGCCCATGAATCTGGCACTCTTTGACCTCGACCACACCCTGATCCCGACCGACAGCGACCATGAAT‑GGGCCGCTTCCTGGTCCGCCTGGGCGTCGTCGACGAGGAAACCTACCGGCAGAAGAACGACGAGTTCTACGGCCACTACAAAGCCGGCACCCTCGATATCCAGGCCTTCCTGCGCTTTGCGCTGGCGCCGCTGGCGGCCAACCCGCGCGACC > AM260479/3333618‑3333912
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
