Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F1 I1 R1
|
152 |
24.9 |
2083062 |
91.6% |
1908084 |
109.2 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
AM260480 |
919,006 |
T→G |
C159G (TGC→GGC) |
h16_B0812 → |
hydrolase or acyltransferase (alpha/beta hydrolase superfamily) |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | AM260480 | 919,006 | 0 | T | G | 100.0%
| 79.5
/ NA
| 22 | C159G (TGC→GGC) | h16_B0812 | hydrolase or acyltransferase (alpha/beta hydrolase superfamily) |
| Reads supporting (aligned to +/- strand): ref base T (0/0); new base G (10/12); total (10/12) |
GCCGGCCTGGCGGCCTGGCCAGACTGGTGCTGGTCGACCCGCCGGTGTCGGGGCCCGGGCGCCGCGCGTATCCTGCGCAACTGCCGTGGTATGTGGATTCCATCCGCCTGTCGCGCCAGTGCACCGACGTGGAAGGCATGCGCCGCTTCTGCCCGACCTGGACCGACGAGCAGCTGCGCCTGCGCGCCGAATGGCTGCATACCTGCAACGAAGACGCCGTGGTGCGCAGCTTTGAGGATTTCCATCG > AM260480/918887‑919133
|
gccggccTGGCGGCCTGGCCAGACTGGTGCTGGTCGACCCGCCGGTGTCGGGGCCCGGGCGCCGCGCGTATCCTGCGCAACTGCCGTGGTATGTGGATTCCATCCGCCTGTCGCGCCAGGGCACCGACGTGGaa < 1:103488/134‑1 (MQ=255)
ggTCGACCCGCCGGTGTCGGGGCCCGGGCGCCGCGCGTATCCTGCGCAACTGCCGTGGTATGTGGATTCCATCCGCCTGTCGCGCCAGGGCACCGACGTGGAAGGCATGcgcc > 2:905581/1‑113 (MQ=255)
ggTCGACCCGCCGGTGTCGGGGCCCGGGCGCCGCGCGTATCCTGCGCAACTGCCGTGGTATGTGGATTCCATCCGCCTGTCGCGCCAGGGCACCGACGTGGAAGGCATGcgcc < 1:905581/113‑1 (MQ=255)
ggTCGACCCGCCGGTGTCGGGGCCCGGGCGCCGCGCGTATCCTGCGCAACTGCCGTGGTATGTGGATTCCATCCGCCTGTCGCGCCAGGGCACCGACGTGGAAGGCATGCGCCGCtt < 2:9082/117‑1 (MQ=255)
ggTCGACCCGCCGGTGTCGGGGCCCGGGCGCCGCGCGTATCCTGCGCAACTGCCGTGGTATGTGGATTCCATCCGCCTGTCGCGCCAGGGCACCGACGTGGAAGGCATGCGCCGCtt > 1:9082/1‑117 (MQ=255)
ggTCGACCCGCCGGTGTCGGGGCCCGGGCGCCGCGCGTATCCTGCGCAACTGCCGTGGTATGTGGATTCCATCCGCCTGTCGCGCCAGGGCACCGACGTGGAAGGCATGCGCCGCTTCTGCCCGACCTGGAcc > 2:798619/1‑133 (MQ=255)
gTCGACCCGCCGGTGTCGGGGCCCGGGCGCCGCGCGTATCCTGCGCAACTGCCGTGGTATGTGGATTCCATCCGCCTGTCGCGCCAGGGCACCGACGTGGAAGGCATGCGCCGCTTCTGCCCGACCTGGACcg < 2:903714/133‑1 (MQ=255)
ccgGTGTCGGGGCCCGGGCGCCGCGCGTATCCTGCGCAACTGCCGTGGTATGTGGATTCCATCCGCCTGTCGCGCCAGGGCACCGACGTGGAAGGCATGCGCCGCtt > 1:714987/1‑107 (MQ=255)
ccgGTGTCGGGGCCCGGGCGCCGCGCGTATCCTGCGCAACTGCCGTGGTATGTGGATTCCATCCGCCTGTCGCGCCAGGGCACCGACGTGGAAGGCATGCGCCGCtt < 2:714987/107‑1 (MQ=255)
ccGGGCGCCGCGCGTATCCTGCGCAACTGCCGTGGTATGTGGATTCCATCCGCCTGTCGCGCCAGGGCACCGACGTGGAAGGCATGCGCCGCTTCTGCCCGACCTGGACCGACGAGCAGCTGCGCCTGCGCGcc < 1:608752/134‑1 (MQ=255)
cgcgcgTATCCTGCGCAACTGCCGTGGTATGTGGATTCCATCCGCCTGTCGCGCCAGGGCACCGACGTGGAAGGCATGCGCCGCTTCTGCCCGACCTGGACCGACGAGCAGCTGCGCCTGCGCGCCGAATGGCt < 2:210823/134‑1 (MQ=255)
aCTGCCGTGGTATGTGGATTCCATCCGCCTGTCGCGCCAGGGCACCGACGTGGAAGGCATGCGCCGCTTCTGCCCGAcc > 1:1038259/1‑79 (MQ=255)
aCTGCCGTGGTATGTGGATTCCATCCGCCTGTCGCGCCAGGGCACCGACGTGGAAGGCATGCGCCGCTTCTGCCCGAcc < 2:1038259/79‑1 (MQ=255)
tATGTGGATTCCATCCGCCTGTCGCGCCAGGGCACCGACGTGGAAGGCATGCGCCGCTTCTGCCCGACCTGGACCGACGAGCAGCTGCGCCTGCGCGCCGAATGGCTGCATACCTGCAACGAAGACGCCgtggt < 2:127664/134‑1 (MQ=255)
gATTCCATCCGCCTGTCGCGCCAGGGCACCGACGTGGAAGGCATGCGCCGCTTCTGCCCGACCTGGACCGACGAGCAGCTGCGCCTGCGCGCCGAATGGCTGCATACCTGCAACGAAGACGCCgtgg < 2:655530/127‑1 (MQ=255)
gATTCCATCCGCCTGTCGCGCCAGGGCACCGACGTGGAAGGCATGCGCCGCTTCTGCCCGACCTGGACCGACGAGCAGCTGCGCCTGCGCGCCGAATGGCTGCATACCTGCAACGAAGACGCCgtgg > 1:655530/1‑127 (MQ=255)
ccATCCGCCTGTCGCGCCAGGGCACCGACGTGGAAGGCATGCGCCGCTTCTGCCCGACCTGGACCGACGAGCAGCTGCGCCTGCGCGCCGAATGGCTGCATACCTGCAACGAAGACGCCGTGGTGCGCAGCttt > 1:26728/1‑134 (MQ=255)
tCCGCCTGTCGCGCCAGGGCACCGACGTGGAAGGCATGCGCCGCTTCTGCCCGACCTGGACCGACGAGCAGCTGCGCCTGCGCGCCGaa > 1:969579/1‑89 (MQ=255)
tCCGCCTGTCGCGCCAGGGCACCGACGTGGAAGGCATGCGCCGCTTCTGCCCGACCTGGACCGACGAGCAGCTGCGCCTGCGCGCCGaa < 2:969579/89‑1 (MQ=255)
tCCGCCTGTCGCGCCAGGGCACCGACGTGGAAGGCATGCGCCGCTTCTGCCCGACCTGGACCGACGAGCAGCTGCGCCTGCGCGCCGAATGGCGGCATACCTGCAACGAAGACGCCGTGGTGCGCAGCTTTGAg > 2:318035/1‑134 (MQ=255)
gcCAGGGCACCGACGTGGAAGGCATGCGCCGCTTCTGCCCGACCTGGACCGACGAGCAGCTGCGCCTGCGCGCCGAATGGCTGCATACCTGCAACGAAGACGCCGTGGTGCGCAGCTTTGAGGATTTCCATcg < 2:384951/133‑1 (MQ=255)
gcCAGGGCACCGACGTGGAAGGCATGCGCCGCTTCTGCCCGACCTGGACCGACGAGCAGCTGCGCCTGCGCGCCGAATGGCTGCATACCTGCAACGAAGACGCCGTGGTGCGCAGCTTTGAGGATTTCCATcg > 1:384951/1‑133 (MQ=255)
|
GCCGGCCTGGCGGCCTGGCCAGACTGGTGCTGGTCGACCCGCCGGTGTCGGGGCCCGGGCGCCGCGCGTATCCTGCGCAACTGCCGTGGTATGTGGATTCCATCCGCCTGTCGCGCCAGTGCACCGACGTGGAAGGCATGCGCCGCTTCTGCCCGACCTGGACCGACGAGCAGCTGCGCCTGCGCGCCGAATGGCTGCATACCTGCAACGAAGACGCCGTGGTGCGCAGCTTTGAGGATTTCCATCG > AM260480/918887‑919133
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
BRESEQ :: bam2aln output
GTGCGCGCCGCGGCGGGCCGGCCTGGCGGCCTGGCCAGACTGGTGCTGGTCGACCCGCCGGTGTCGGGGCCCGGGCGCCGCGCGTATCCTGCGCAACTGCCGTGGTATGTGGATTCCATCCGCCTGTCGCGCCAGTGCACCGACGTGGAAGGCATGCGCCGCTTCTGCCCGACCTGGACCGACGAGCAGCTGCGCCTGCGCGCCGAATGGCTGCATACCTGCAACGAAGACGCCGTGGTGCGCAGCTTTGAGGATTTCCATCGCGACGACATCCATGCGGATCT > AM260480/918871‑919154
|
gaagacggcatacgagatgtagctccgtctcgtgggctcggagatgtgtataagagacaGGTGTCGGGGCCCGGGCGCCGCGCGTATCCTGCGCAACTGCCGTGGTATGTGGATTCCATCCGCCTGTCGCGCCAGGGCACCGACGTGGAA < GWNJ‑0478:712:GW2002102894th:2:1102:5532:74911/91‑1 (MQ=60)
tttttttttttttcaagcagaagacggcatacgagatgtagctccgtctcgtgggctcggagatgtgtataagagacaGCGCGTATCCTGCGCAACTGCCGTGGTATGTGGATTCCATCCGCCTGTCGCGCCAGGGCACCGACGTGGAA < GWNJ‑0478:712:GW2002102894th:2:1103:10165:63093/71‑1 (MQ=60)
GGCCGGCCTGGCGGCCTGGCCAGACTGGTGCTGGTCGACCCGCCGGTGTCGGGGCCCGGGCGCCGCGCGTATCCTGCGCAACTGCCGTGGTATGTGGATTCCATCCGCCTGTCGCGCCAGGGCACCGACGTGGAAGGCATGCGCCGCTTC < GWNJ‑0478:712:GW2002102894th:2:1108:12661:3229/150‑1 (MQ=60)
gagacagGGCCAGACTGGTGCTGGTCGACCCGCCGGTGTCGGGGCCCGGGCGCCGCGCGTATCCTGCGCAACTGCCGTGGTATGTGGATTCCATCCGCCTGTCGCGCCAGGGCACCGACGTGGAAGGCATGCGCCGCTTCTGCCCGACCT < GWNJ‑0478:712:GW2002102894th:2:2208:9649:44257/143‑1 (MQ=60)
GGCCAGACTGGTGCTGGTCGACCCGCCGGTGTCGGGGCCCGGGCGCCGCGCGTATCCTGCGCAACTGCCGTGGTATGTGGATTCCATCCGCCTGTCGCGCCAGGGCACCGACGTGGAAGGCATGCGCCGCTTCTGCCCGACCTGGACCtg > GWNJ‑0478:712:GW2002102894th:2:1101:12418:67692/1‑148 (MQ=60)
GGTGCTGGTCGACCCGCCGGTGTCGGGGCCCGGGCGCCGCGCGTATCCTGCGCAACTGCCGTGGTATGTGGATTCCATCCGCCTGTCGCGCCAGGGCACCGACGTGGAAGctgtctcttatacacatctccgagcccacgagacggagct > GWNJ‑0478:712:GW2002102894th:2:2115:5500:55581/1‑110 (MQ=60)
GTGCTGGTCGACCCGCCGGTGTCGGGGCCCGGGCGCCGCGCGTATCCTGCGCAACTGCCGTGGTATGTGGATTCCATCCGCCTGTCGCGCCAGGGCACCGACGTGGAAGGCATGCGCCGCTTCTGCCCGACCTGGACCtgtctcttatac > GWNJ‑0478:712:GW2002102894th:2:2112:13876:44301/1‑138 (MQ=60)
CCCGGGCGCCGCGCGTATCCTGCGCAACTGCCGTGGTATGTGGATTCCATCCGCCTGTCGCGCCAGGGCACCGACGTGGAAGGCATGCGCCGCTTCTGCCCGACCTGGACCGACGAGCAGCTGCGCCTGCGCGCCGAATGGCTGCATACC < GWNJ‑0478:712:GW2002102894th:2:2107:4187:97736/150‑1 (MQ=60)
CGCGTATCCTGCGCAACTGCCGTGGTATGTGGATTCCATCCGCCTGTCGCGCCAGGGCACCGACGTGGAAGGCATGCGCCGCTTCTGCCCGACCTGGACCGACGAGCAGCTGtctcttatacacatctccgagcccacgagacggagct > GWNJ‑0478:712:GW2002102894th:2:2216:18783:82618/1‑112 (MQ=60)
CTGCCGTGGTATGTGGATTCCATCCGCCTGTCGCGCCAGGGCACCGACGTGGAAGGCATGCGCCGCTTCTGCCCGACCTGGACCGACGAGCAGCTGCGCCTGCGCGCCGAATGGCTGCATACCTGCAACGAAGACGCCGTGGTGCGCAGC > GWNJ‑0478:712:GW2002102894th:2:2109:6988:97900/1‑150 (MQ=60)
CGTGGTATGTGGATTCCATCCGCCTGTCGCGCCAGGGCACCGACGTGGAAGGCATGCGCCGCTTCTGCCCGACCTGGACCGACGAGCAGCTGCGCCTGCGCGCCGAATGGCTGCATACCTGCAACGAAGACGCCGTGGTGCGCAGCTTTG > GWNJ‑0478:712:GW2002102894th:2:1103:10351:89053/1‑150 (MQ=60)
GGTATGTGGATTCCATCCGCCTGTCGCGCCAGGGCACCGACGTGGAAGGCATGCGCCGCTTCTGCCCGACCTGGACCGACGAGCAGCTGCGCCTGCGCGCCGAATGGCTGCATACCTGCctgtctcttatacacatctccgagcccacg > GWNJ‑0478:712:GW2002102894th:2:2213:4488:16425/1‑119 (MQ=60)
CCATCCGCCTGTCGCGCCAGGGCACCGACGTGGAAGGCATGCGCCGCTTCTGCCCGACCTGGACCGACGAGCAGCTGCGCCTGCGCGCCGAATGGCTGCATACCTGCAACGAAGACGCCGTGGTGCGCAGCTTTGAGGATTTCCATCGCG > GWNJ‑0478:712:GW2002102894th:2:1209:6728:41155/1‑150 (MQ=60)
GGGCACCGACGTGGAAGGCATGCGCCGCTTCTGCCCGACCTGGACCtgtctcttatacacatctccgagcccacgagacggagctacatctcgtatgccgtcttctgcttgaaaaaaaaaaaaaaaccaacataatagaacacgaatctc > GWNJ‑0478:712:GW2002102894th:2:2205:9210:23203/1‑46 (MQ=14)
|
GTGCGCGCCGCGGCGGGCCGGCCTGGCGGCCTGGCCAGACTGGTGCTGGTCGACCCGCCGGTGTCGGGGCCCGGGCGCCGCGCGTATCCTGCGCAACTGCCGTGGTATGTGGATTCCATCCGCCTGTCGCGCCAGTGCACCGACGTGGAAGGCATGCGCCGCTTCTGCCCGACCTGGACCGACGAGCAGCTGCGCCTGCGCGCCGAATGGCTGCATACCTGCAACGAAGACGCCGTGGTGCGCAGCTTTGAGGATTTCCATCGCGACGACATCCATGCGGATCT > AM260480/918871‑919154
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
