Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F1 I1 R1
|
152 |
24.9 |
2083062 |
91.6% |
1908084 |
109.2 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
AM260480 |
951,073 |
+G |
intergenic (‑29/+230) |
h16_B0838 ← / ← h16_B0839 |
conserved hypothetical protein/transcriptional regulator, CRP‑family |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | AM260480 | 951,073 | 1 | . | G | 100.0%
| 101.3
/ NA
| 27 | intergenic (‑29/+230) | h16_B0838/h16_B0839 | conserved hypothetical protein/transcriptional regulator, CRP‑family |
| Reads supporting (aligned to +/- strand): ref base . (0/0); new base G (15/12); total (15/12) |
GGGCTGGGCGGACTGCACCGGCTGACTGGATTGCATCGGCTGCATCGGCTGCGTCATTGGCATCGGCTGGCTCGGCTGCAGGGGCTGAGCCT‑CTGCGCAAACAGCGGGTAGGACGCGATCATCGCGCCAGCCACGACCAGGGATTTCGCCAGATGTTGCTTCATTCGTGTACCTCCTAGAACGCCTACAGGCAAGGGAACAGTCACTGTAGC > AM260480/950982‑951193
|
gggCTGGGCGGACTGCACCGGCTGACTGGATTGCATCGGCTGCATCGGCTGCGTCATTGGCATCGGCTGGCTCGGCTGCAGGGGCTGAGCCTGCTGCGCAAAc < 1:140065/103‑1 (MQ=255)
gggCTGGGCGGACTGCACCGGCTGACTGGATTGCATCGGCTGCATCGGCTGCGTCATTGGCATCGGCTGGCTCGGCTGCAGGGGCTGAGCCTGCTGCGCAAAc > 2:140065/1‑103 (MQ=255)
ggCTGGGCGGACTGCACCGGCTGACTGGATTGCATCGGCTGCATCGGCTGCGTCATTGGCATCGGCTGGCTCGGCTGCAGGGGCTGAGCCTGCTgcgc > 1:604242/1‑98 (MQ=255)
ggCTGGGCGGACTGCACCGGCTGACTGGATTGCATCGGCTGCATCGGCTGCGTCATTGGCATCGGCTGGCTCGGCTGCAGGGGCTGAGCCTGCTgcgc < 2:604242/98‑1 (MQ=255)
gCTGGGCGGACTGCACCGGCTGACTGGATTGCATCGGCTGCATCGGCTGCGTCATTGGCATCGGCTGGCTCGGCTGCAGGGGCTGAGCCTGCTGCGCAAACAGCGGGTAGGACGCGATCATCGCGCCAGCCAc > 1:910300/1‑133 (MQ=255)
ggCGGACTGCACCGGCTGACTGGATTGCATCGGCTGCATCGGCTGCGTCATTGGCATCGGCTGGCTCGGCTGCAGGGGCTGAGCCTGCTGCGCAAACAGCGGGTAGGACGCGATCATCGCGCCAGCCACGAc > 1:477941/1‑132 (MQ=255)
cGGCTGACTGGATTGCATCGGCTGCATCGGCTGCGTCATTGGCATCGGCTGGCTCGGCTGCAGGGGCTGAGCCTGCTGCGCAAACAGCGGGTAGGACGCGATCATCGCGCCAGCCACGACCAGGGATTTCGCCa < 1:890839/134‑1 (MQ=255)
cTGGATTGCATCGGCTGCATCGGCTGCGTCATTGGCATCGGCTGGCTCGGCTGCAGGGGCTGAGCCTGCTGCGCAAACAGCGGGTAGGACGCGATCATCGCGCCAGCCACGACCAGGGATTTCGCCAGATGTTg < 1:484907/134‑1 (MQ=255)
aTTGCATCGGCTGCATCGGCTGCGTCATTGGCATCGGCTGGCTCGGCTGCAGGGGCTGAGCctgct < 2:78913/66‑4 (MQ=255)
aTTGCATCGGCTGCATCGGCTGCGTCATTGGCATCGGCTGGCTCGGCTGCAGGGGCTGAGCctgct > 1:78913/1‑63 (MQ=255)
gctgcatcggctgcGTCATTGGCATCGGCTGGCTCGGCTGCAGGGGCTGAGCCTGCTGCGCAAACAGCGGGTAGGACGCGATCATCGCGCCAGCCACGACCAGGGATTTCGCCAGATGTTGCTTCATTCGTGTa > 1:616235/1‑134 (MQ=255)
gctgcGTCATTGGCATCGGCTGGCTCGGCTGCAGGGGCTGAGCCTGCTGCGCAAACAGCGGGTAGGACGCGATCATCGCGCCAGCCACGACCAGGGATTTCGCCAGATGTTGCTTCATTCGTGTACCTCCTAg > 2:364938/1‑133 (MQ=255)
cGTCATTGGCATCGGCTGGCTCGGCTGCAGGGGCTGAGCCTGCTGCGCAAACAGCGGGTAGGACGCGATCATCGCGCCAGCCACGACCAGGGATTTCGCCAGATGTTGCTTCATTc > 1:19231/1‑116 (MQ=255)
cGTCATTGGCATCGGCTGGCTCGGCTGCAGGGGCTGAGCCTGCTGCGCAAACAGCGGGTAGGACGCGATCATCGCGCCAGCCACGACCAGGGATTTCGCCAGATGTTGCTTCATTCGTGTACCTCCTAGAACg < 2:477941/133‑1 (MQ=255)
cGTCATTGGCATCGGCTGGCTCGGCTGCAGGGGCTGAGCCTGCTGCGCAAACAGCGGGTAGGACGCGATCATCGCGCCAGCCACGACCAGGGATTTAGCCAGATGTTGCTTCATTc < 2:19231/116‑1 (MQ=255)
ttGGCATCGGCTGGCTCGGCTGCAGGGGCTGAGCCTGCTGCGCAAACAGCGGGTAGGACGCGATCATCGCGCCAGCCACGACCAGGGATTTCGCCAGATGTTGCTTCATTCGTGTAcctcc < 1:774650/121‑1 (MQ=255)
ttGGCATCGGCTGGCTCGGCTGCAGGGGCTGAGCCTGCTGCGCAAACAGCGGGTAGGACGCGATCATCGCGCCAGCCACGACCAGGGATTTCGCCAGATGTTGCTTCATTCGTGTAcctcc > 2:576384/1‑121 (MQ=255)
ttGGCATCGGCTGGCTCGGCTGCAGGGGCTGAGCCTGCTGCGCAAACAGCGGGTAGGACGCGATCATCGCGCCAGCCACGACCAGGGATTTCGCCAGATGTTGCTTCATTCGTGTAcctcc > 2:774650/1‑121 (MQ=255)
ttGGCATCGGCTGGCTCGGCTGCAGGGGCTGAGCCTGCTGCGCAAACAGCGGGTAGGACGCGATCATCGCGCCAGCCACGACCAGGGATTTCGCCAGATGTTGCTTCATTCGTGTAcctcc < 1:576384/121‑1 (MQ=255)
aTCGGCTGGCTCGGCTGCAGGGGCTGAGCCTGCTGCGCAAACAGCGGGTAGGACGCGATCATCGCGCCAGCCACGACCAGGGATTTCGCCAGATGTTGCTTCAtt < 2:813804/105‑1 (MQ=255)
aTCGGCTGGCTCGGCTGCAGGGGCTGAGCCTGCTGCGCAAACAGCGGGTAGGACGCGATCATCGCGCCAACCACGACCAGGGATTTCGCCAGATGTTGCTTCAtt > 1:813804/1‑105 (MQ=255)
gctggctCGGCTGCAGGGGCTGAGCCTGCTGCGCAAACAGCGGGTAGGACGCGATCATCGCGCCAGCCACGACCAGGGATTTCGCCAGATGtt < 2:246406/93‑1 (MQ=255)
gctggctCGGCTGCAGGGGCTGAGCCTGCTGCGCAAACAGCGGGTAGGACGCGATCATCGCGCCAGCCACGACCAGGGATTTCGCCAGATGtt > 1:246406/1‑93 (MQ=255)
ctggctCGGCTGCAGGGGCTGAGCCTGCTGCGCAAACAGCGGGTAGGACGCGATCATCGCGCCAGCCACGACCAGGGATTTCGCCAGATGTTGCTTCATTCGTGTAcctc > 2:767070/1‑110 (MQ=255)
ctggctCGGCTGCAGGGGCTGAGCCTGCTGCGCAAACAGCGGGTAGGACGCGATCATCGCGCCAGCCACGACCAGGGATTTCGCCAGATGTTGCTTCATTCGTGTAcctc < 1:767070/110‑1 (MQ=255)
gCTGCAGGGGCTGAGCCTGCTGCGCAAACAGCGGGTAGGACGCGATCATCGCGCCAGCCACGACCAGGGATTTCGCCAGATGTTGCTTCATTCGTGTACCTCCTAGAACGCCTACAGGCAAgg > 2:574081/1‑123 (MQ=255)
gCTGCAGGGGCTGAGCCTGCTGCGCAAACAGCGGGTAGGACGCGATCATCGCGCCAGCCACGACCAGGGATTTCGCCAGATGTTGCTTCATTCGTGTACCTCCTAGAACGCCTACAGGCAAgg < 1:574081/123‑1 (MQ=255)
gCTGCAGGGGCTGAGCCTGCTGCGCAAACAGCGGGTAGGACGCGATCATCGCGCCAGCCACGACCAGGGATTTCGCCAGATGTTGCTTCATTCGTGTACCTCCTAGAACGCCTACAGGCAAGGGAACAGTCACt > 1:909549/1‑134 (MQ=255)
aGGGGCTGAGCCTGCTGCGCAAACAGCGGGTAGGACGCGATCATCGCGCCAGCCACGACCAGGGATTTCGCCAGATGTTGCTTCATTCGTGTACCTCCTAGAACGCCTACAGGCAAGGGAACAGTCACTGTAgc > 2:251190/1‑134 (MQ=255)
|
GGGCTGGGCGGACTGCACCGGCTGACTGGATTGCATCGGCTGCATCGGCTGCGTCATTGGCATCGGCTGGCTCGGCTGCAGGGGCTGAGCCT‑CTGCGCAAACAGCGGGTAGGACGCGATCATCGCGCCAGCCACGACCAGGGATTTCGCCAGATGTTGCTTCATTCGTGTACCTCCTAGAACGCCTACAGGCAAGGGAACAGTCACTGTAGC > AM260480/950982‑951193
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
BRESEQ :: bam2aln output
CCCGCTGCGGGTGCCACCTGGGCCGACGGCGCTGCCGACGGCATGGCTTGCACGGGCTGGGCGGACTGCACCGGCTGACTGGATTGCATCGGCTGCATCGGCTGCGTCATTGGCATCGGCTGGCTCGGCTGCAGGGGCTGAGCCT‑CTGCGCAAACAGCGGGTAGGACGCGATCATCGCGCCAGCCACGACCAGGGATTTCGCCAGATGTTGCTTCATTCGTGTACCTCCTAGAACGCCTACAGGCAAGGGAACAGTCACTGTAGCGCCGCAGCACCTGACATTCTGTTTGAT > AM260480/950929‑951220
|
cgtgggctcggagatgtgtataagagacagGCTGCCGACGGCATGGCTTGCACGGGCTGGGCGGACTGCACCGGCTGACTGGATTGCATCGGCTGCATCGGCTGCGTCATTGGCATCGGCTGGCTCGGCTGCAGGGGCTGAGCCTGCTG < GWNJ‑0478:712:GW2002102894th:2:1203:12886:73441/119‑1 (MQ=60)
atgtgtataagagacagCGGCATGGCTTGCACGGGCTGGGCGGACTGCACCGGCTGACTGGATTGCATCGGCTGCATCGGCTGCGTCATTGGCATCGGCTGGCTCGGCTGCAGGGGCTGAGCCTGCTGCGCAAACAGCGGGTAGGACGCG < GWNJ‑0478:712:GW2002102894th:2:1111:2320:27494/133‑1 (MQ=60)
GGCATGGCTTGCACGGGCTGGGCGGACTGCACCGGCTGACTGGATTGCATCGGCTGCATCGGCTGCGTCATTGGCATCGGCTGGCTCGGCTGCAGGGGCTGAGCCTGCTGCGCAAACAGCGGGTAGGACtgtctcttatacacatctccg > GWNJ‑0478:712:GW2002102894th:2:2107:5599:69983/1‑129 (MQ=60)
GCATGGCTTGCACGGGCTGGGCGGACTGCACCGGCTGACTGGATTGCATCGGCTGCATCGGCTGCGTCATTGGCATCGGCTGGCTCGGCTGCAGGGGCTGAGCCTGCTGCGCAAACAGCGGGTAGGACGCGATCATCGCGCCAGCCACG > GWNJ‑0478:712:GW2002102894th:2:2208:4497:73100/1‑149 (MQ=60)
GGCTTGCACGGGCTGGGCGGACTGCACCGGCTGACTGGATTGCATCGGCTGCATCGGCTGCGTCATTGGCATCGGCTGGCTCGGCTGCAGGGGCTGAGCCTGCTGCGCAAACAGCGGGTAGGACGCGATCATCGCGCCAGCCACGACC > GWNJ‑0478:712:GW2002102894th:2:1215:16468:54989/1‑148 (MQ=60)
GCACCGGCTGACTGGATTGCATCGGCTGCATCGGCTGCGTCATTGGCATCGGCTGGCTCGGCTGCAGGGGCTGAGCCTGCTGCGCAAACAGCGGGTctgtctcttatacacatctccgagcccacgagacggagctacatctcgtatgcc > GWNJ‑0478:712:GW2002102894th:2:1107:9181:44435/1‑96 (MQ=60)
CCGGCTGACTGGATTGCATCGGCTGCATCGGCTGCGTCATTGGCATCGGCTGGCTCGGCTGCAGGGGCTGAGCCTGCTGCGCAAACAGCGGGTAGGACGCGATCATCGCGCCAGCCACGACCAGGGATTTCGCCAGATGTTGCTTCATTC < GWNJ‑0478:712:GW2002102894th:2:2207:16400:50042/150‑1 (MQ=60)
GACTGGATTGCATCGGCTGCATCGGCTGCGTCATTGGCATCGGCTGGCTCGGCTGCAGGGGCTGAGCCTGCTGCGCAAACAGCGGGTAGGACGCGATCATCGCGCCAGCCACGACCAGGGATTTCGCCAGATGTTGCTTCATTCGTGTAC > GWNJ‑0478:712:GW2002102894th:2:2106:13254:51322/1‑150 (MQ=60)
ACTGGATTGCATCGGCTGCATCGGCTGCGTCATTGGCATCGGCTGGCTCGGCTGCAGGGGCTGAGCCTGCTGCGCAAACAGCGGGTAGGACGCGATCATCGCGCCAGCCACGACCAGGGATTTCGCCAGATGTTGCTTCATTCGTGTACC < GWNJ‑0478:712:GW2002102894th:2:1215:11756:90729/150‑1 (MQ=60)
CGGCTGCATCGGCTGCGTCATTGGCATCGGCTGGCTCGGCTGCAGGGGCTGAGCCTGCTGCGCAAACAGCGGGTAGGACGCGATCATCGCGCCAGCCACGACCAGGGATTTCGCCAGATGTTGCTTCATTCGTGTACCTCCTAGAACtgt > GWNJ‑0478:712:GW2002102894th:2:1103:15040:39296/1‑147 (MQ=60)
aagagacaGCGTCATTGGCATCGGCTGGCTCGGCTGCAGGGGCTGAGCCTGCTGCGCAAACAGCGGGTAGGACGCGATCATCGCGCCAGCCACGACCAGGGATTTCGCCAGATGTTGCTTCATTCGTGTACCTCCTAGAACGCCTACAG < GWNJ‑0478:712:GW2002102894th:2:2116:8642:35984/141‑1 (MQ=60)
CATCGGCTGCGTCATTGGCATCGGCTGGCTCGGCTGCAGGGGCTGAGCCTGCTGCGCAAACAGCGGGTAGGACGCGATCATCGCGCCAGCCACGACCAGGGATTTCGCCAGATGTTGCTTCATTCGTGTACCTCCTAGAACGCCTACAGG < GWNJ‑0478:712:GW2002102894th:2:2105:3182:83016/150‑1 (MQ=60)
CATCGGCTGCGTCATTGGCATCGGCTGGCTCGGCTGCAGGGGCTGAGCCTGCTGCGCAAACAGCGGGTAGGACGCGATCATCGCGCCAGCCACGACCAGGGATTTCGCCAGATGTTGCTTCATTCGTGTACCTCCTAGAACGCCTACAGG < GWNJ‑0478:712:GW2002102894th:2:2116:2164:78711/150‑1 (MQ=60)
GGCTGCGTCATTGGCATCGGCTGGCTCGGCTGCAGGGGCTGAGCCTGCTGCGCAAACAGCGGGTAGGACGCGATCATCGCGCCAACCACGACCAGGGATTTCGCCAGATGTTGCTTCATTCGTGTACCTCCTAGActgtctcttatacac > GWNJ‑0478:712:GW2002102894th:2:2202:15574:51382/1‑135 (MQ=60)
GCGTCATTGGCATCGGCTGGCTCGGCTGCAGGGGCTGAGCCTGCTGCGCAAACAGCGGGTAGGACGCGATCATCGCGCCAGCCACGACCAGGGATTTCGCCAGATGTTGCTTCATTCGTGTACCTgtctcttatacacatctccgagccc > GWNJ‑0478:712:GW2002102894th:2:1116:15343:27921/1‑125 (MQ=60)
GCATCGGCTGGCTCGGCTGCAGGGGCTGAGCCTGCTGCGCAAACAGCGGGTAGGACGCGATCATCGCGCCAGCCACGACCAGGGATTTCGCCAGATGTTGCTTCATTCGTGTACCTCCTAGAACGCCTACAGGCAAGGGAACAGTCACTG > GWNJ‑0478:712:GW2002102894th:2:2208:20091:68684/1‑150 (MQ=60)
CGGCTGGCTCGGCTGCAGGGGCTGAGCCTGCTGCGCAAACAGCGGGTAGGACGCGATCATCGCGCCAGCCACGACCAGGGATTTCGCCAGATGTTGCTTCATTCGTGTACCTCCTAGAACGCCTACAGGCAAGGGAACAGTCACTGTAG < GWNJ‑0478:712:GW2002102894th:2:2105:9859:66944/149‑1 (MQ=60)
G‑CTGCGCAAACAGCGGGTAGGACGCGATCATCGCGCCAGCCACGACCAGGGATTTCGCCAGATGTTGCTTCATTCGTGTACCTCCTAGAACGCCTACAGGCAAGGGAACAGTCACTGTAGCGCCGCAGCACCTGACATTCTGTTTGAT < GWNJ‑0478:712:GW2002102894th:2:1201:11650:21448/148‑1 (MQ=60)
|
CCCGCTGCGGGTGCCACCTGGGCCGACGGCGCTGCCGACGGCATGGCTTGCACGGGCTGGGCGGACTGCACCGGCTGACTGGATTGCATCGGCTGCATCGGCTGCGTCATTGGCATCGGCTGGCTCGGCTGCAGGGGCTGAGCCT‑CTGCGCAAACAGCGGGTAGGACGCGATCATCGCGCCAGCCACGACCAGGGATTTCGCCAGATGTTGCTTCATTCGTGTACCTCCTAGAACGCCTACAGGCAAGGGAACAGTCACTGTAGCGCCGCAGCACCTGACATTCTGTTTGAT > AM260480/950929‑951220
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
