Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F1 I1 R1
|
152 |
24.9 |
2083062 |
91.6% |
1908084 |
109.2 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
AM260480 |
2,295,879 |
T→A |
Q603L (CAG→CTG) |
h16_B2042 ← |
Hypothetical protein |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | AM260480 | 2,295,879 | 0 | T | A | 100.0%
| 52.1
/ NA
| 15 | Q603L (CAG→CTG) | h16_B2042 | Hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base T (0/0); new base A (8/7); total (8/7) |
CACTGGCGTTGCTGGGCGTCGCGTTGCCGGGCCTCCTGCTGTTGCCGCTGCAGATCAGGCGCCTGCCGCTGCTGCTCGCGCAGCGCGCGCTGCTGTTCCCGCTGCTGCTCCATCTGCTGGCGCTGCAAAACCTGCTGCTGTTCGCGCTGCATGCCCTGCGCCCGCCGTTGCGGGTCGGCCGGTCCGCCCGGCTGGCCCGGCTGGCCAACAAAGCCGCGGGC > AM260480/2295774‑2295994
|
cACTGGCGTTGCTGGGCGTCGCGTTGCCGGGCCTCCTGCTGTTGCCGCTGCAGATCAGGCGCCTGCCGCTGCTGCTCGCGCAGCGCGCGCTGCTGTTCCCGCTGCAGCTCCATCTGCTGGCGCTGCAAAACctg > 1:230698/1‑134 (MQ=255)
gTTGCTGGGCGTCGCGTTGCCGGGCCTCCTGCTGTTGCCGCTGCAGATCAGGCGCCTGCCGCTGCTGCTCGCGCAGCGCGCGCTGCTGTTCCCGCTGCAGCTCCATCTGCTGGCGCTGCAAAACCTGCTGCTGt < 2:979404/134‑1 (MQ=255)
gggCGTCGCGTTGCCGGGCCTCCTGCTGTTGCCGCTGCAGATCAGGCGCCTGCCGCTGCTGCTCGCGCAGCGCGCGCTGCTGTTCCCGCTGCAGCTCCATctg > 1:402722/1‑103 (MQ=255)
gggCGTCGCGTTGCCGGGCCTCCTGCTGTTGCCGCTGCAGATCAGGCGCCTGCCGCTGCTGCTCGCGCAGCGCGCGCTGCTGTTCCCGCTGCAGCTCCATctg < 2:402722/103‑1 (MQ=255)
cgcgTTGCCGGGCCTCCTGCTGTTGCCGCTGCAGATCAGGCGCCTGCCGCTGCTGCTCGCGCAGCGCGCGCTGCTGTTCCCGCTGCAGCTCCATCTGCTGGCGCTGCAAAACCTGCTGCTGTTCGCGCTGCATg > 1:620546/1‑134 (MQ=255)
ccGGGCCTCCTGCTGTTGCCGCTGCAGATCAGGCGCCTGCCGCTGCTGCTCGCGCAGCGCGCGCTGCTGTTCCCGCTGCAGCTCCATCTGCTGGCGCTGCAAAACCTGCTGCTGTTCGCGCTGCATGCCCTgcg < 1:978500/134‑1 (MQ=255)
ccGGGCCTCCTGCTGTTGCCGCTGCAGATCAGGCGCCTGCCGCTGCTGCTCGCGCAGCGCGCGCTGCTGTTCCCGCTGCAGCTCCATCTGCTGGCGCTGCAAAACCTGCTGCTGTTCGCGCTGCATGCCCTgcg < 2:1025113/134‑1 (MQ=255)
cTGCCGCTGCTGCTCGCGCAGCGCGCGCTGCTGTTCCCGCTGCAGCTCCATctg > 1:426767/1‑54 (MQ=255)
cTGCCGCTGCTGCTCGCGCAGCGCGCGCTGCTGTTCCCGCTGCAGCTCCATctg < 2:426767/54‑1 (MQ=255)
gctgctgctCGCGCAGCGCGCGCTGCTGTTCCCGCTGCAGCTCCATCTGCTGGCGCTGCAAAACCTGCTGCTGTTCGCGCTGCATGCCCTGCGCCCGCCGTTGCGGGTCGGCCGGTCCgcccggctggcccggc > 2:526916/1‑134 (MQ=255)
cgcAGCGCGCGCTGCTGTTCCCGCTGCAGCTCCATCTGCTGGCGCTGCAAAACCTGCTGCTGTTCGCGCTGCATGCCCTGCGCCCGCCGTTGCGGGTCGGCCGGTCCGCCCGGCTGGCCCGGCTGGCCAACaaa < 1:320517/134‑1 (MQ=255)
cAGCGCGCGCTGCTGTTCCCGCTGCAGCTCCATCTGCTGGCGCTGCAAAACCTGCTGCTGTTCGCGCTGCATGCCCTGCGCCCGCCGTTGCGGGTCGGCCGGTCCGCCCGGCTGGCCCGGCTGGCCAACAAAGc > 2:266073/1‑134 (MQ=255)
cgcTGCTGTTCCCGCTGCAGCTCCATCTGCTGGCGCTGCAAAACCTGCTGCTGTTCGCGCTGCATGCCCTGCGCCCGCCGTTGCGGGTCGGCCGGTCCGCCCGGCTGGCCCGGCTGGCCAACAAAGCCGCGGGc > 2:386709/1‑134 (MQ=255)
cccGCTGCAGCTCCATCTGCTGGCGCTGCAAAACCTGCTGCTGt > 1:238992/1‑44 (MQ=255)
cccGCTGCAGCTCCATCTGCTGGCGCTGCAAAACCTGCTGCTGt < 2:238992/44‑1 (MQ=255)
|
CACTGGCGTTGCTGGGCGTCGCGTTGCCGGGCCTCCTGCTGTTGCCGCTGCAGATCAGGCGCCTGCCGCTGCTGCTCGCGCAGCGCGCGCTGCTGTTCCCGCTGCTGCTCCATCTGCTGGCGCTGCAAAACCTGCTGCTGTTCGCGCTGCATGCCCTGCGCCCGCCGTTGCGGGTCGGCCGGTCCGCCCGGCTGGCCCGGCTGGCCAACAAAGCCGCGGGC > AM260480/2295774‑2295994
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
BRESEQ :: bam2aln output
CCTGCTGGCGTTGCTGGTCGCGGGCCTGGTCCTGCCACTGGCGTTGCTGGGCGTCGCGTTGCCGGGCCTCCTGCTGTTGCCGCTGCA‑‑‑‑GATCAGGCGCCTGCCGCTGCTGCTCGCGCAGCGCGCGCTGCTGTTCCCGCTGCTGCTCCATCTGCTGGCGCTGCAAAACCTGCTGCTGTTCGCGCTGCATGCCCTGCGCCCGCCGTTGCGGGTCGGCCGGTCCGCCCGGCTGGCCCGGCTGGCCAACAAAGCCGCGGGCTTCGTCGGGCCGGCTGCT > AM260480/2295739‑2296012
|
CCTGCTGGCGTTGCTGGTCGCGGGCCTGGTCCTGCCACTGGCGTTGCTGGGCGTCGCGTTGCCGGGCCTCCTGCTGTTGCCGCTGCA‑‑‑‑GATCAGGCGCCTGCCGCTGCTGCTCGCGCAGCGCGCGCTGCTGTTCCCGCTGCAGCTCCATCT < GWNJ‑0478:712:GW2002102894th:2:2202:19997:30961/150‑1 (MQ=60)
CGGGCCTGGTCCTGCCACTGGCGTTGCTGGGCGTCGCGTTGCCGGGCCTCCTGCTGTTGCCGCTGCA‑‑‑‑GATCAGGCGCCTGCCGCTGCTGCTCGCGCAGCGCGCGCTGCTGTTCCCGCTGCAGCTCCATCTGCTGGCGCTGCAAAACCTGC > GWNJ‑0478:712:GW2002102894th:2:1114:11264:41125/1‑150 (MQ=60)
GCCACTGGCGTTGCTGGGCGTCGCGTTGCCGGGCCTCCTGCTGTTGCCGCTGCA‑‑‑‑GATCAGGCGCCTGCCGCTGCTGCTCGCGCAGCGCGCGCTGCTGTTCCCGCTGCAGCTCCATCTGCTGGCGCTGCAAAACCTGtctcttatacacat > GWNJ‑0478:712:GW2002102894th:2:1211:11548:43230/1‑136 (MQ=60)
GGCGTTGCTGGGCGTCGCGTTGCCGGGCCTCCTGCTGTTGCCGCTGCA‑‑‑‑GATCAGGCGCCTGCCGCTGCTGCTCGCGCAGCGCGCGCTGCTGTTCCCGCTGCAGCTCCATCTGCTGGCGCTGCAAAACCTGCTGCTGTTCGCGCTGCATGC > GWNJ‑0478:712:GW2002102894th:2:2106:7771:79737/1‑150 (MQ=60)
GGGCGTCGCGTTGCCGGGCCTCCTGCTGTTGCCGCTGCAGCGGGAACA‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑GCAGCGCGCGCTGCTGTTCCCGCTGCAGCTCCATCTGCTGGCGCTGCAAAACCTGCTGCTGTTCGCGCTGCATGCCCTGCGCCCGCCGTTGCGGACCGGCCG < GWNJ‑0478:712:GW2002102894th:2:2216:9599:28009/150‑1 (MQ=60)
GCCGGGCCTCCTGCTGTTGCCGCTGCA‑‑‑‑GATCAGGCGCCTGCCGCTGCTGCTCGCGCAGCGCGCGCTGCTGTTCCCGCTGCAGCTCCATCTGCTGGCGCTGCAAAACCTGCTGCTGTTCGCGCTGCATGCCCTGCGCCCGCCGTTGCGGGT < GWNJ‑0478:712:GW2002102894th:2:2213:6358:63978/150‑1 (MQ=60)
CTGCA‑‑‑‑GATCAGGCGCCTGCCGCTGCTGCTCGCGCAGCGCGCGCTGCTGTTCCCGCTGCAGCTCCATCTGCTGGCGCTGCAAAACCTGtctcttatacacatctccgagcccacgagacggagctacatctcgtatgccgtcttctgcttg > GWNJ‑0478:712:GW2002102894th:2:1210:20949:79228/1‑87 (MQ=60)
GCGCAGCGCGCGCTGCTGTTCCCGCTGCAGCTCCATCTGCTGGCGCTGCAAAACCTGCTGCTGTTCGCGCTGCATGCCCTGCGCCCGCCGTTGCGGGTCGGCCGGTCCGCCCGGCTGGCCCGGCTGGCCAACAAAGCCGCGGGCTTCGTC < GWNJ‑0478:712:GW2002102894th:2:1204:6047:32870/150‑1 (MQ=60)
GCGCGCGCTGCTGTTCCCGCTGCAGCTCCATCTGCTGGCGCTGCAAAACCTGCTGCTGTTCGCGCTGCATGCCCctgtctcttatacacatctccgagcccacgagacggagctacatctcgtatgccgtcttctgcttgaaaaaaaaa > GWNJ‑0478:712:GW2002102894th:2:1114:20944:86489/1‑74 (MQ=60)
CTGCTGTTCCCGCTGCAGCTCCATCTGCTGGCGCTGCAAAACCTGCTGCTGTTCGCGCTGCATGCCCTGCGCCCGCCGTTGCGGGTCGGCCGGTCCGCCCGGCTGGCCCGGCTGGCCAACAAAGCCGCGGGCTTCGTCGGGCCGGCTGCT > GWNJ‑0478:712:GW2002102894th:2:2111:15839:45692/1‑150 (MQ=60)
CTGCTGTTCCCGCTGCAGCTCCATCTGCTGGCGCTGCAAAACCTGCTGCTGTTCGCGCTGCATG > GWNJ‑0478:712:GW2002102894th:2:1108:2617:68759/1‑64 (MQ=60)
|
CCTGCTGGCGTTGCTGGTCGCGGGCCTGGTCCTGCCACTGGCGTTGCTGGGCGTCGCGTTGCCGGGCCTCCTGCTGTTGCCGCTGCA‑‑‑‑GATCAGGCGCCTGCCGCTGCTGCTCGCGCAGCGCGCGCTGCTGTTCCCGCTGCTGCTCCATCTGCTGGCGCTGCAAAACCTGCTGCTGTTCGCGCTGCATGCCCTGCGCCCGCCGTTGCGGGTCGGCCGGTCCGCCCGGCTGGCCCGGCTGGCCAACAAAGCCGCGGGCTTCGTCGGGCCGGCTGCT > AM260480/2295739‑2296012
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
