Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A0 F0 I1 R1
|
155 |
25.8 |
2285122 |
88.0% |
2010907 |
104.3 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
AM260479 |
1,056,947 |
+G |
intergenic (+211/‑15) |
h16_A0964 → / → h16_A0965 |
conserved hypothetical protein/conserved hypothetical protein |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | AM260479 | 1,056,945 | 1 | . | G | 100.0%
| 68.3
/ NA
| 19 | intergenic (+209/‑17) | h16_A0964/h16_A0965 | conserved hypothetical protein/conserved hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base . (0/0); new base G (10/9); total (10/9) |
GCTTCTGTTGCAGATGCTGCACAAATGGCAACCGCCGGCGGCGCCGGCATTGGGGGCATGGTGGCGACACGTCAGGAGGGCGGGCAAGCCGTGGCCAAGGGTACTGTCGAGGCCCGGCGCGAGCGCGGCA‑GGCCGCCCGCCAGCAGGTGCCGCGCGGTGCGCACGAGGCCATCGGCAACGTCGACCGCGACCCCGTCGAACTGCTGGAAATCAGCAGCCAGGGCCGGGTTGAGCGGCTGGTGCCGCT > AM260479/1056816‑1057062
|
gCTTCTGTTGCAGATGCTGCACAAATGGCAACCGCCGGCGGCGCCGGCATTGGGGGCATGGTGGCGACACGTCAGGAGGGCGGGCAAGCCGTGGCCAAGGGTACTGTCGAGGCCCGGCGCGAGCGCGGCA‑ggg < 1:559486/133‑2 (MQ=255)
ttGCAGATGCTGCACAAATGGCAACCGCCGGCGGCGCCGGCATTGGGGGCATGGTGGCGACACGTCAGGAGGGCGGGCAAGCCGTGGCCAAGGGTACTGTCGAGGCCCGGCGCGAGCGCGGCAGGGccgcccgc > 1:679488/1‑134 (MQ=255)
gCAGATGCTGCACAAATGGCAACCGCCGGCGGCGCCGGCATTGGGGGCATGGTGGCGACACGTCAGGAGGGCGGGCAAGCCGTGGCCAAGGGTACTGTCGAGGCCCGGCGCGAGCGCGGCAGGGccgcccgc > 2:1089610/1‑132 (MQ=255)
cacaAATGGCAACCGCCGGCGGCGCCGGCATTGGGGGCATGGTGGCGACACGTCAGGAGGGCGGGCAAGCCGTGGCCAAGGGTACTGTCGAGGCCCGGCGCGAGCGCGGCAGGGCCGCCCGCCAGCAGGTGc < 2:679488/132‑1 (MQ=255)
cacaAATGGCAACCGCCGGCGGCGCCGGCATTGGGGGCATGGTGGCGACACGTCAGGAGGGCGGGCAAGCCGTGGCCAAGGGTACTGTCGAGGCCCGGCGCGAGCGCGGCAGGGCCGCCCGCCAGCAGGTGc < 1:968246/132‑1 (MQ=255)
aaaTGGCAACCGCCGGCGGCGCCGGCATTGGGGGCATGGTGGCGACACGTCAGGAGGGCGGGCAAGCCGTGGCCAAGGGTACTGTCGAGGCCCGGCGCGAGCGCGGCAGGGCCGCCCGCCAGCAGGTGCcgcgc < 1:1089610/134‑1 (MQ=255)
gccgGCGGCGCCGGCATTGGGGGCATGGTGGCGACACGTCAGGAGGGCGGGCAAGCCGTGGCCAAGGGTACTGTCGAGGCCCGGCGCGAGCGCGGCAGGGCCGCCCGCCAGCAGGTGc < 1:533414/118‑1 (MQ=255)
gccgGCGGCGCCGGCATTGGGGGCATGGTGGCGACACGTCAGGAGGGCGGGCAAGCCGTGGCCAAGGGTACTGTCGAGGCCCGGCGCGAGCGCGGCAGGGCCGCCCGCCAGCAGGTGc > 2:533414/1‑118 (MQ=255)
cacGTCAGGAGGGCGGGCAAGCCGTGGCCAAGGGTACTGTCGAGGCCCGGCGCGAGCGCGGCAGGGCCGCCCGCCAGCAGGTGCCGCGCGGTgcg < 1:363/95‑1 (MQ=255)
cacGTCAGGAGGGCGGGCAAGCCGTGGCCAAGGGTACTGTCGAGGCCCGGCGCGAGCGCGGCAGGGCCGCCCGCCAGCAGGTGCCGCGCGGTgcg > 2:363/1‑95 (MQ=255)
ggaggGCGGGCAAGCCGTGGCCAAGGGTACTGTCGAGGCCCGGCGCGAGCGCGGCAGGGCCGCCCGCCAGCAGGTGCCGCGCGGTgcg < 1:1133027/88‑1 (MQ=255)
ggaggGCGGGCAAGCCGTGGCCAAGGGTACTGTCGAGGCCCGGCGCGAGCGCGGCAGGGCCGCCCGCCAGCAGGTGCCGCGCGGTgcg > 2:1133027/1‑88 (MQ=255)
cAAGCCGTGGCCAAGGGTACTGTCGAGGCCCGGCGCGAGCGCGGCAGGGCCGCCCGCCAGCAGGTGCCGCGCGGTGCGCACGAGGCCATCGGCAACGTCGACCGCGACCCCGTCGAACTGCTGGAAATcagcag > 1:971543/1‑134 (MQ=255)
aGCCGTGGCCAAGGGTACTGTCGAGGCCCGGCGCGAGCGCGGCAGGGCCGCCCGCCAGCAGGTGCCGCGCGGTGCGCACGAGGCCATCGGCAACGTCGACCGCGACCCCGTCGAACTGCTGGAAATcag > 1:745236/1‑129 (MQ=255)
aGCCGTGGCCAAGGGTACTGTCGAGGCCCGGCGCGAGCGCGGCAGGGCCGCCCGCCAGCAGGTGCCGCGCGGTGCGCACGAGGCCATCGGCAACGTCGACCGCGACCCCGTCGAACTGCTGGAAATcag < 2:745236/129‑1 (MQ=255)
gAGGCCCGGCGCGAGCGCGGCAGGGCCGCCCGCCAGCAGGTGCCGCGCGGTGCGCACGAGGCCATCGGCAACGTCGACCGCGACCCCGTCGAACTGCTGGAAATCAGCAGCCAGGGCCGGGTTGAGCGGCTGGt < 2:971543/134‑1 (MQ=255)
ccGGCGCGAGCGCGGCAGGGCCGCCCGCCAGCAGGTGCCGCGCGGTGCGCACGAGGCCATCGGCAACGTCGACCGCGACCCCGTCGAACTGCTGGAAATCAGCAGCCAGGGCCGGGTTGAGCGGCTGGTg > 2:1056893/1‑130 (MQ=255)
ccGGCGCGAGCGCGGCAGGGCCGCCCGCCAGCAGGTGCCGCGCGGTGCGCACGAGGCCATCGGCAACGTCGACCGCGACCCCGTCGAACTGCTGGAAATCAGCAGCCAGGGCCGGGTTGAGCGGCTGGTg < 1:1056893/130‑1 (MQ=255)
ccGGCGCGAGCGCGGCAGGGCCGCCCGCCAGCAGGTGCCGCGCGGTGCGCACGAGGCCATCGGCAACGTCGACCGCGACCCCGTCGAACTGCTGGAAATCAGCAGCCAGGGCCGGGTTGAGCGGCTGGTGCCGc > 1:771228/1‑134 (MQ=255)
cGGCGCGAGCGCGGCAGGGCCGCCCGCCAGCAGGTGCCGCGCGGTGCGCACGAGGCCATCGGCAACGTCGACCGCGACCCCGTCGAACTGCTGGAAATCAGCAGCCAGGGCCGGGTTGAGCGGCTGGTGCCGCt > 1:952204/1‑134 (MQ=255)
|
GCTTCTGTTGCAGATGCTGCACAAATGGCAACCGCCGGCGGCGCCGGCATTGGGGGCATGGTGGCGACACGTCAGGAGGGCGGGCAAGCCGTGGCCAAGGGTACTGTCGAGGCCCGGCGCGAGCGCGGCA‑GGCCGCCCGCCAGCAGGTGCCGCGCGGTGCGCACGAGGCCATCGGCAACGTCGACCGCGACCCCGTCGAACTGCTGGAAATCAGCAGCCAGGGCCGGGTTGAGCGGCTGGTGCCGCT > AM260479/1056816‑1057062
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
BRESEQ :: bam2aln output
GTGCATTAGCTTCTGTTGCAGATGCTGCACAAATGGCAACCGCCGGCGGCGCCGGCATTGGGGGCATGGTGGCGACACGTCAGGAGGGCGGGCAAGCCGTGGCCAAGGGTACTGTCGAGGCCCGGCGCGAGCGCGGCA‑GGCCGCCCGCCAGCAGGTGCCGCGCGGTGCGCACGAGGCCATCGGCAACGTCGACCGCGACCCCGTCGAACTGCTGGAAATCAGCAGCCAGGGCCGGGTTGAGCGGCTGGTGCCGCTGCGCTACGGCCGCATGATCCAGTCGC > AM260479/1056808‑1057088
|
GTGCATTAGCTTCTGTTGCAGATGCTGCACAAATGGCAACCGCCGGCGGCGCCGGCATTGGGGGCATGGTGGCGACACGTCAGGAGGGCGGGCAAGCCGTGGCCAAGGGTACTGTCGAGGCCCGGCGCGAGCGCGGCAGGGCCGCCCGCC > GWNJ‑0478:712:GW2002102894th:2:2106:12434:66222/1‑150 (MQ=60)
AGCTTCTGTTGCAGATGCTGCACAAATGGCAACCGCCGGCGGCGCCGGCATTGGGGGCATGGTGGCGACACGTCAGGAGGGCGGGCAAGCCGTGGCCAAGGGTACTGTCGAGGCCCGGCGCGAGCGCGGCA‑GGgcctcgacagtaccct < GWNJ‑0478:712:GW2002102894th:2:1216:17981:22425/149‑17 (MQ=60)
agGCACAAATGGCAACCGCCGGCGGCGCCGGCATTGGGGGCATGGTGGCGACACGTCAGGAGGGCGGGCAAGCCGTGGCCAAGGGTACTGTCGAGGCCCGGCGCGAGCGCGGCAGGGCCGCCCGCCAGCAGGTGCCGCGCGGTGCGCACG < GWNJ‑0478:712:GW2002102894th:2:1215:15718:95322/148‑1 (MQ=60)
GCACAAATGGCAACCGCCGGCGGCGCCGGCATTGGGGGCATGGTGGCGACACGTCAGGAGGGCGGGCAAGCCGTGGCCAAGGGTACTGTCGAGGCCCGGCGCGAGCGCGGCAGGGCCGCCCGCCAGCAGGTGCCGCGCGGTGCGCACG < GWNJ‑0478:712:GW2002102894th:2:2206:19759:91922/148‑1 (MQ=60)
CAAATGGCAACCGCCGGCGGCGCCGGCATTGGGGGCATGGTGGCGACACGTCAGGAGGGCGGGCAAGCCGTGGCCAAGGGTACTGTCGAGGCCCGGCGCGAGCGCGGCAGGGCCGCCCGCCAGCAGGTGCCGCGCGGTGCGCACGAGGCC < GWNJ‑0478:712:GW2002102894th:2:2214:17975:43621/150‑1 (MQ=60)
gctcggagatgtgtataagagacaGGGGGCATGGTGGCGACACGTCAGGAGGGCGGGCAAGCCGTGGCCAAGGGTACTGTCGAGGCCCGGCGCGAGCGCGGCAGGGCCGCCCGCCAGCAGGTGCCGCGCGGTGCGCACGAGGCCATCGGC < GWNJ‑0478:712:GW2002102894th:2:1101:4154:4528/126‑1 (MQ=60)
cgtgggctcggagatgtgtataagagacagGGTGGCGACACGTCAGGAGGGCGGGCAAGCCGTGGCCAAGGGTACTGTCGAGGCCCGGCGCGAGCGCGGCAGGGCCGCCCGCCAGCAGGTGCCGCGCGGTGCGCACGAGGCCATCGGC < GWNJ‑0478:712:GW2002102894th:2:2216:18259:52697/118‑1 (MQ=60)
ATTGGGGGCATGGTGGCGACACGTCAGGAGGGCGGGCAAGCCGTGGCCAAGGGTACTGTCGAGGCCCGGCGCGAGCGCGGCA‑GGgcctgtctcttatacacatctccgagcccacgagacactcgctaatctcgtatgccgtcttctgct > GWNJ‑0478:712:GW2002102894th:2:1111:3990:37598/1‑84 (MQ=60)
agcagaagacggcatacgagattagcgagtgtctcgtgggctcggagatgtgtataagagacagcggcagGGCCGCCCGCCAGCAGGTGCCGCGCGGTGCGCACGAGGCCATCGGCAACGTCGACCGCGACCCCGTCGAACTGCTGGAAA < GWNJ‑0478:712:GW2002102894th:2:2214:11366:73449/80‑1 (MQ=60)
CGTCAGGAGGGCGGGCAAGCCGTGGCCAAGGGTACTGTCGAGGCCCGGCGCGAGCGCGGCAGGGCCGCCCGCCAGCAGGTGCCGCGCGGTGCGCACGAGGCCATCGGCAACGTCGACCGCGACCCCGTCGAACTGCTGGAAATCAGCAGC > GWNJ‑0478:712:GW2002102894th:2:2207:18170:11694/1‑150 (MQ=60)
TCAGGAGGGCGGGCAAGCCGTGGCCAAGGGTACTGTCGAGGCCCGGCGCGAGCGCGGCAGGGCCGCCCGCCAGCAGGTGCCGCGCGGTGCGCACGAGGCCATCGGCAACGTCGACCGCGACCCCGTCGAACTGCTGGAAATCAGCAGCC > GWNJ‑0478:712:GW2002102894th:2:2110:2090:33051/1‑149 (MQ=60)
tagcgagtgtctcgtgggctcggagatgtgtataagagacagcggcagGGCCGCCCGCCAGCAGGTGCCGCGCGGTGCGCACGAGGCCATCGGCAACGTCGACCGCGACCCCGTCGAACTGCTGGAAATCAGCAACCAGGGCCGGGTTG < GWNJ‑0478:712:GW2002102894th:2:1114:19695:93671/101‑1 (MQ=60)
GGGTACTGTCGAGGCCCGGCGCGAGCGCGGCAGGGCCGCCCGCCAGCAGctgtctcttatacacatctccgagcccacgagacactcgctaatctcgtatgccgtcttctgcttgaaaaaaaaaaaaaacaaaaactagaataacaaaa > GWNJ‑0478:712:GW2002102894th:2:1210:4088:67506/1‑49 (MQ=60)
GGGTACTGTCGAGGCCCGGCGCGAGCGCGGCAGGGCCGCCCGCCAGCAGGTGCCGCGCGGTGCGCACGAGGCCATCGGCAACGTCGACCGCGACCCCGTCGAACTGCTGGAAATCAGCAGCCAGGGCCGGGTTGAGCGGCTGGTGCCGCT > GWNJ‑0478:712:GW2002102894th:2:2113:15639:71197/1‑150 (MQ=60)
GGTACTGTCGAGGCCCGGCGCGAGCGCGGCAGGGCCGCCCGCCAGCAGGTGCCGCGCGGTGCGCACGAGGCCATCGGCAACGTCGACCGCGACCCCGTCGAACTGCTGGAAATCAGCAGCCAGGGCCGGGTTGAGCGGCTGGTGCCGCTG > GWNJ‑0478:712:GW2002102894th:2:2209:5133:97718/1‑150 (MQ=60)
GAGGCCCGGCGCGAGCGCGGCAGGGCCGCCCGCCAGCAGGTGCCGCGCGGTGCGCACGAGGCCATCGGCAACGTCGACCGCGACCCCGTCGAACTGCTGGAAATCAGCAGCCAGGGCCGGGTTGAGCGGCTGGTGCCGCTGCGCTACGGC < GWNJ‑0478:712:GW2002102894th:2:2212:9370:78722/150‑1 (MQ=60)
CGGCAGGGCCGCCCGCCAGCAGGTGCCGCGCGGTGCGCACGAGGCCATCGGCAACGTCGACCGCGACCCCGTCGAACTGCTGGAAATCAGCAGCCAGGGCCtgtctcttatacacatctccgagcccacgagacactcgctaatctcgt > GWNJ‑0478:712:GW2002102894th:2:1210:8233:64139/1‑101 (MQ=60)
|
GTGCATTAGCTTCTGTTGCAGATGCTGCACAAATGGCAACCGCCGGCGGCGCCGGCATTGGGGGCATGGTGGCGACACGTCAGGAGGGCGGGCAAGCCGTGGCCAAGGGTACTGTCGAGGCCCGGCGCGAGCGCGGCA‑GGCCGCCCGCCAGCAGGTGCCGCGCGGTGCGCACGAGGCCATCGGCAACGTCGACCGCGACCCCGTCGAACTGCTGGAAATCAGCAGCCAGGGCCGGGTTGAGCGGCTGGTGCCGCTGCGCTACGGCCGCATGATCCAGTCGC > AM260479/1056808‑1057088
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
