Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F1 I1 R1
|
152 |
24.9 |
2083062 |
91.6% |
1908084 |
109.2 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
AM260479 |
1,056,947 |
+G |
intergenic (+211/‑15) |
h16_A0964 → / → h16_A0965 |
conserved hypothetical protein/conserved hypothetical protein |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | AM260479 | 1,056,945 | 1 | . | G | 100.0%
| 58.8
/ NA
| 16 | intergenic (+209/‑17) | h16_A0964/h16_A0965 | conserved hypothetical protein/conserved hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base . (0/0); new base G (9/7); total (9/7) |
CACAAATGGCAACCGCCGGCGGCGCCGGCATTGGGGGCATGGTGGCGACACGTCAGGAGGGCGGGCAAGCCGTGGCCAAGGGTACTGTCGAGGCCCGGCGCGAGCGCGGCA‑GGCCGCCCGCCAGCAGGTGCCGCGCGGTGCGCACGAGGCCATCGGCAACGTCGACCGCGACCCCGTCGAACTGCTGGAAATCAGCAGCCAGGGCCGGGTTGAGCGGCTGGTGCCGCTGCGCTACG > AM260479/1056835‑1057070
|
cacaAATGGCAACCGCCGGCGGCGCCGGCATTGGGGGCATGGTGGCGACACGTCAGGAGGGCGGGCAAGCCGTGGCCAAGGGTACTGTCGAGGCCCGGCGCGAGCGCGGCAGGGCCGCCCGCCAGCAGGTGc < 2:866625/132‑1 (MQ=255)
cacaAATGGCAACCGCCGGCGGCGCCGGCATTGGGGGCATGGTGGCGACACGTCAGGAGGGCGGGCAAGCCGTGGCCAAGGGTACTGTCGAGGCCCGGCGCGAGCGCGGCAGGGCCGCCCGCCAGCAGGTGCcg > 1:630727/1‑134 (MQ=255)
aaTGGCAACCGCCGGCGGCGCCGGCATTGGGGGCATAGTGGCGACACGTCAGGAGGGCGGGCAAGCCGTGGCCAAGGGTACTGTCGAGGCCCGGCGCGAGCGCGGCAGGGCCGCCCGCCAGCAGGTGCcgcgcg > 1:932602/1‑134 (MQ=255)
ccgccgGCGGCGCCGGCATTGGGGGCATGGTGGCGACACGTCAGGAGGGCGGGCAAGCCGTGGCCAAGGGTACTGTCGAGGCCCGGCGCGAGCGCGGCAGGGCCGCCCGCCAGCAGGTGCCGCGCGGTGCGCAc > 2:70237/1‑134 (MQ=255)
gccgGCGGCGCCGGCATTGGGGGCATGGTGGCGACACGTCAGGAGGGCGGGCAAGCCGTGGCCAAGGGTACTGTCGAGGCCCGGCGCGAGCGCGGCAGGGCCGCCCGCCAGCAGGTGCCGCGCGGTGCGCACGa < 2:630727/134‑1 (MQ=255)
ggcgCCGGCATTGGGGGCATGGTGGCGACACGTCAGGAGGGCGGGCAAGCCGTGGCCAAGGGTACTGTCGAGGCCCGGCGCGAGCGCGGCAGGGCCGCCCGCCAGCAGGTGCCGCGCGGTGCGCACGAGGCCAt < 2:356852/134‑1 (MQ=255)
acacGTCAGGAGGGCGGGCAAGCCGTGGCCAAGGGTACTGTCGAGGCCCGGCGCGAGCGCGGCAGGGCCGCCCGCCAGCAGGTGCCGCGCGGTGCGCACGAGGCCATCGGc < 1:409341/111‑1 (MQ=255)
acacGTCAGGAGGGCGGGCAAGCCGTGGCCAAGGGTACTGTCGAGGCCCGGCGCGAGCGCGGCAGGGCCGCCCGCCAGCAGGTGCCGCGCGGTGCGCACGAGGCCATCGGc > 2:409341/1‑111 (MQ=255)
gggcAAGCCGTGGCCAAGGGTACTGTCGAGGCCCGGCGCGAGCGCGGCAGGGCCGCCCGCCAGCAGGTGCCGCGCGGTGCGCACGAGGCCATCGGCAACGTCGACCGCGACCCCGTCGAACTGCTGGAAATcag < 2:932602/134‑1 (MQ=255)
aaGCCGTGGCCAAGGGTACTGTCGAGGCCCGGCGCGAGCGCGGCAGGGCCGCCCGCCAGCAGGTGCCGCGCGGTGCGCACGAGGCCATCGGCAACGTCGACCGCGACCCCGTCGAACTGCTGGAAATCagcagc > 1:551268/1‑134 (MQ=255)
gTACTGTCGAGGCCCGGCGCGAGCGCGGCAGGGCCGCCCGCCAGCAGGTGCCGCGCGGTGCGCACGAGGCCATCGGCAACGTCGACCGCGACCCCGTCGAACTGCTGGAAATCAGCAGCCAGGGCCGGGTTGAg < 2:551268/134‑1 (MQ=255)
cccGGCGCGAGCGCGGCAGGGCCGCCCGCCAGCAGGTGCCGCGCGGTGCGCACGAGGCCATCGGCAACGTCGACCGCGACCCCGTCGAACTGCTGGAAATCAGCAGCCAGGGCCGGGTTGAGCGGCTGGTg > 1:666627/1‑131 (MQ=255)
cccGGCGCGAGCGCGGCAGGGCCGCCCGCCAGCAGGTGCCGCGCGGTGCGCACGAGGCCATCGGCAACGTCGACCGCGACCCCGTCGAACTGCTGGAAATCAGCAGCCAGGGCCGGGTTGAGCGGCTGGTg < 2:666627/131‑1 (MQ=255)
gAGCGCGGCAGGGCCGCCCGCCAGCAGGTGCCGCGCGGTGCGCACGAGGCCATCGGCAACGTCGACCGCGACCCCGTCGAACTGCTGGAAATCAGCAGCCAGGGCCGGGTTGAGCGGCTGGTGCCGCTGCGCTa > 1:864566/1‑134 (MQ=255)
gAGCGCGGCAGGGCCGCCCGCCAGCAGGTGCCGCGCGGTGCGCACGAGGCCATCGGCAACGTCGACCGCGACCCCGTCGAACTGCTGGAAATCAGCAGCCAGGGCCGGGTTGAGCGGCTGGTGCCGCTGCGCTa > 2:893444/1‑134 (MQ=255)
gcgcGGCAGGGCCGCCCGCCAGCAGGTGCCGCGCGGTGCGCACGAGGCCATCGGCAACGTCGACCGCGACCCCGTCGAACTGCTGGAAATCAGCAGCCAGGGCCGGGTTGAGCGGCTGGTGCCGCTGCGCTACg > 1:488038/1‑134 (MQ=255)
|
CACAAATGGCAACCGCCGGCGGCGCCGGCATTGGGGGCATGGTGGCGACACGTCAGGAGGGCGGGCAAGCCGTGGCCAAGGGTACTGTCGAGGCCCGGCGCGAGCGCGGCA‑GGCCGCCCGCCAGCAGGTGCCGCGCGGTGCGCACGAGGCCATCGGCAACGTCGACCGCGACCCCGTCGAACTGCTGGAAATCAGCAGCCAGGGCCGGGTTGAGCGGCTGGTGCCGCTGCGCTACG > AM260479/1056835‑1057070
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
BRESEQ :: bam2aln output
GCGGCGCAGTGCATTAGCTTCTGTTGCAGATGCTGCACAAATGGCAACCGCCGGCGGCGCCGGCATTGGGGGCATGGTGGCGACACGTCAGGAGGGCGGGCAAGCCGTGGCCAAGGGTACTGTCGAGGCCCGGCGCGAGCGCGGCA‑GGCCGCCCGCCAGCAGGTGCCGCGCGGTGCGCACGAGGCCATCGGCAACGTCGACCGCGACCCCGTCGAACTGCTGGAAATCAGCAGCCAGGGCCGGGTTGAGCGGCTGGTGCCGCTGCGCTACGG > AM260479/1056800‑1057071
|
gtgggctcggagatgtgtataagagaCAGATGCTGCACAAATGGCAACCGCCGGCGGCGCCGGCATTGGGGGCATGGTGGCGACACGTCAGGAGGGCGGGCAAGCCGTGGCCAAGGGTACTGTCGAGGCCCGGCGCGAGCGCGGCA‑GGGC < GWNJ‑0478:712:GW2002102894th:2:2213:9291:93344/124‑1 (MQ=60)
CTGTTGCAGATGCTGCACAAATGGCAACCGCCGGCGGCGCCGGCATTGGGGGCATGGTGGCGACACGTCAGGAGGGCGGGCAAGCCGTGGCCAAGGGTACTGTCGAGGCCCGGCGCGAGCGCGGCAGGGCCGCCCGCCAGCAGGTGCCGC > GWNJ‑0478:712:GW2002102894th:2:2108:9050:44715/1‑150 (MQ=60)
ttgttttttctcgcgttctttttttttttttcaagcagaagacggcatacgagatgtagctccgtctcgtgggctcggagatgtgtataagagacagGTCGAGGCCCGGCGCGAGCGCGGCAGGGCCGCCCGCCAGCAGGTGCCGCGC < GWNJ‑0478:712:GW2002102894th:2:1214:15062:88219/51‑1 (MQ=60)
TGCAGATGCTGCACAAATGGCAACCGCCGGCGGCGCCGGCATTGGGGGCATAGTGGCGACACGTCAGGAGGGCGGGCAAGCCGTGGCCAAGGGTACTGTCGAGGCCCGGCGCGAGCGCGGCAGGGCCGCCCGCCAGCAGGTGCCGCGCGG > GWNJ‑0478:712:GW2002102894th:2:2210:3532:4346/1‑150 (MQ=60)
aagagacagGGGGGCATGGTGGCGACACGTCAGGAGGGCGGGCAAGCCGTGGCCAAGGGTACTGTCGAGGCCCGGCGCGAGCGCGGCAGGGCCGCCCGCCAGCAGGTGCCGCGCGGTGCGCACGAGGCCATCGGCAACGTCGACCGCGAC < GWNJ‑0478:712:GW2002102894th:2:1211:14218:79740/141‑1 (MQ=60)
GTCAGGAGGGCGGGCAAGCCGTGGCCAAGGGTACTGTCGAGGCCCGGCGCGAGCGCGGCAGGGCCGCCCGCCAGCAGGTGCCGCGCGGTGCGCACGAGGCCATCGGCAACGTCGACCGCGACCCCGTCGAACTGCTGGAAATCAGCAGCC > GWNJ‑0478:712:GW2002102894th:2:2103:4398:99468/1‑150 (MQ=60)
AGGGTACTGTCGAGGCCCGGCGCGAGCGCGGCAGGGCCGCCCGCCAGCAGGTGCCGCGCGGTGCGCACGAGGCCATCGGCAACGTCGACCGCGACCCCGTCGAACTGCTGGAAATCAGCAGCCAGGGCCGGGTTGAGCGGCTGGTGCCGC > GWNJ‑0478:712:GW2002102894th:2:2111:12731:63514/1‑150 (MQ=60)
GTCGAGGCCCGGCGCGAGCGCGGCAGGGCCGCCCGCCAGCAGGTGCCGCGCGGTGCGCACGAGGCCATCGGCAACGTCGACCGCGACCCCGTCGAACTGCTGGAAATCAGCAGCCAGGGCCGGGTTGAGCGGCTGGTGCCGCTGCGCTAC > GWNJ‑0478:712:GW2002102894th:2:2209:13481:84105/1‑150 (MQ=60)
ggcctcgtgcgcaccGCGCGGCAGGGCCGCCCGCCAGCAGGTGCCGCGCGGTGCGCACGAGGCCATCGGCAACGTCGACCGCGACCCCGTCGAACTGCTGGAAATCAGCAGCCAGGGCCGGGTTGAGCGGCTGGTGCCGCTGCGCTACGG > GWNJ‑0478:712:GW2002102894th:2:1214:19780:7333/16‑150 (MQ=60)
|
GCGGCGCAGTGCATTAGCTTCTGTTGCAGATGCTGCACAAATGGCAACCGCCGGCGGCGCCGGCATTGGGGGCATGGTGGCGACACGTCAGGAGGGCGGGCAAGCCGTGGCCAAGGGTACTGTCGAGGCCCGGCGCGAGCGCGGCA‑GGCCGCCCGCCAGCAGGTGCCGCGCGGTGCGCACGAGGCCATCGGCAACGTCGACCGCGACCCCGTCGAACTGCTGGAAATCAGCAGCCAGGGCCGGGTTGAGCGGCTGGTGCCGCTGCGCTACGG > AM260479/1056800‑1057071
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
