Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A0 F0 I1 R1
|
155 |
25.8 |
2285122 |
88.0% |
2010907 |
104.3 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
AM260479 |
2,016,501 |
T→C |
V678V (GTT→GTC) |
h16_A1857 → |
Uncharacterized protein involved in exopolysaccharide biosynthesis |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | AM260479 | 2,016,501 | 0 | T | C | 100.0%
| 93.9
/ NA
| 25 | V678V (GTT→GTC) | h16_A1857 | Uncharacterized protein involved in exopolysaccharide biosynthesis |
| Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (11/14); total (11/14) |
TTGCTGCAGCCAGAACTGACCACACTTGTTCAACGCGTTGCCGGCCAGTACGACATGGTGGTGCTTGACGGACCGCCATTGCTGCCGGTGGCAGATGCGCTTGTGCTGGGACGCATGGCGGGCACTGTTTTCATCGTGGCACGGCACCGGGTCACCACGGTGGAACAGATCGACGAAAGCACGCGCCGGCTGGCGCAGGCCGACGTCGCCGTACGGGGCGTGATCTTCAACGACTTCACCGGCCACCTGCACC > AM260479/2016373‑2016625
|
ttGCTGCAGCCAGAACTGACCACACTTGTTCAACGCGTTGCCGGCCAGTACGACATGGTGGTGCTTGACGGACCGCCATTGCTGCCGGTGGCAGATGCGCTTGTGCTGGGACGCATGGCGGGCACTGTCTTc < 2:940104/132‑1 (MQ=255)
ctgcAGCCAGAACTGACCACACTTGTTCAACGCGTTGCCGGCCAGTACGACATGGTGGTGCTTGACGGACCGCCATTGCTGCCGGTGGCAGATGCGCTTGTGCTGGGACGCATGGCGGGCACTGTCTTCATCGt < 2:551444/134‑1 (MQ=255)
aCCACACTTGTTCAACGCGTTGCCGGCCAGTACGACATGGTGGTGCTTGACGGACCGCCATTGCTGCCGGTGGCAGATGCGCTTGTGCTGGGACGCATGGCGGGCACTGTCTTCATCGTGGCACGGCACCggg < 1:282706/133‑1 (MQ=255)
ccACACTTGTTCAACGCGTTGCCGGCCAGTACGACATGGTGGTGCTTGACGGACCGCCATTGCTGCCGGTGGCAGATGCGCTTGTGCTGGGACGCATGGCGGGCACTGTCTTCATCGTGGCACGGCACCGGGTc < 1:191843/134‑1 (MQ=255)
ttGCCGGCCAGTACGACATGGTGGTGCTTGACGGACCGCCATTGCTGCCGGTGGCAGATGCGCTTGTGCTGGGACGCATGGCGGGCACTGTCTTCATCGt < 1:312241/100‑1 (MQ=255)
ttGCCGGCCAGTACGACATGGTGGTGCTTGACGGACCGCCATTGCTGCCGGTGGCAGATGCGCTTGTGCTGGGACGCATGGCGGGCACTGTCTTCATCGt > 2:312241/1‑100 (MQ=255)
ttGCCGGCCAGTACGACATGGTGGTGCTTGACGGACCGCCATTGCTGCCGGTGGCAGATGCGCTTGTGCTGGGACGCATGGCGGGCACTGTCTTCATCGTGGCACGGCACCGGGTCACCACGGTGGaa > 1:669978/1‑128 (MQ=255)
cGGCCAGTACGACATGGTGGTGCTTGACGGACCGCCATTGCTGCCGGTGGCAGATGCGCTTGTGCTGGGACGCATGGCGGGCACTGTCTTCATCGTGGCACGGCACCGGGTCACCACGGTGGAACAGATcgac > 2:128425/1‑133 (MQ=255)
gTACGACATGGTGGTGCTTGACGGACCGCCATTGCTGCCGGTGGCAGATGCGCTTGTGCTGGGACGCATGGCGGGCACTGTCTTCATCGTGGCACGGCACCGGGTCACCACGGTGGAACAGATCGACGAAAGCa > 1:125833/1‑134 (MQ=255)
aCATGGTGGTGCTTGACGGACCGCCATTGCTGCCGGTGGCAGATGCGCTTGTGCTGGGACGCATGGCGGGCACTGTCTTCATCGTggcacg < 1:62969/91‑1 (MQ=255)
aCATGGTGGTGCTTGACGGACCGCCATTGCTGCCGGTGGCAGATGCGCTTGTGCTGGGACGCATGGCGGGCACTGTCTTCATCGTggcacg > 2:62969/1‑91 (MQ=255)
aCATGGTGGTGCTTGACGGACCGCCATTGCTGCCGGTGGCAGATGCGCTTGTGCTGGGACGCATGGCGGGCACTGTCTTCATCGTGGCACGGCACCGGGTCACCACGGTGGAACAGATCGACGAAAGCACgcgc < 2:125833/134‑1 (MQ=255)
ttGACGGACCGCCATTGCTGCCGGTGGCAGATGCGCTTGTGCTGGGACGCATGGCGGGCACTGTCTTCATCGTGGCACGGCACCggg < 1:314152/87‑1 (MQ=255)
ttGACGGACCGCCATTGCTGCCGGTGGCAGATGCGCTTGTGCTGGGACGCATGGCGGGCACTGTCTTCATCGTGGCACGGCACCggg > 2:314152/1‑87 (MQ=255)
aCGGACCGCCATTGCTGCCGGTGGCAGATGCGCTTGTGCTGGGACGCATGGCGGGCACTGTCTTCATCGTg > 1:5934/1‑71 (MQ=255)
aCGGACCGCCATTGCTGCCGGTGGCAGATGCGCTTGTGCTGGGACGCATGGCGGGCACTGTCTTCATCGTg < 2:5934/71‑1 (MQ=255)
aCGGACCGCCATTGCTGCCGGTGGCAGATGCGCTTGTGCTGGGACGCATGGCGGGCACTGTCTTCATCGTGGCACGGCACCGGCTCACCACGGTGGCACAGATCGCCGAAAACACGCGCCGGCTGGAGCAGGcc > 2:1319/1‑134 (MQ=255)
ccGCCATTGCTGCCGGTGGCAGATGCGCTTGTGCTGGGACGCATGGCGGGCACTGTCTTCATCGTGGCACGGCACCggg < 1:746389/79‑1 (MQ=255)
ccGCCATTGCTGCCGGTGGCAGATGCGCTTGTGCTGGGACGCATGGCGGGCACTGTCTTCATCGTGGCACGGCACCggg > 2:746389/1‑79 (MQ=255)
ggCAGATGCGCTTGTGCTGGGACGCATGGCGGGCACTGTCTTCATCGTGGCACGGCACCGGGTCACCACGGTGGAACAGATCGACGAAAGCACgcgc > 2:108558/1‑97 (MQ=255)
ggCAGATGCGCTTGTGCTGGGACGCATGGCGGGCACTGTCTTCATCGTGGCACGGCACCGGGTCACCACGGTGGAACAGATCGACGAAAGCACgcgc < 1:108558/97‑1 (MQ=255)
gCAGATGCGCTTGTGCTGGGACGCATGGCGGGCACTGTCTTCATCGTGGCACGGCACCGGGTCACCACGGTGGAACAGATCGACGAAAGCACGCGCCGGCTGGCGCAGGCCGACGTCGc > 1:824285/1‑119 (MQ=255)
gCAGATGCGCTTGTGCTGGGACGCATGGCGGGCACTGTCTTCATCGTGGCACGGCACCGGGTCACCACGGTGGAACAGATCGACGAAAGCACGCGCCGGCTGGCGCAGGCCGACGTCGc < 2:824285/119‑1 (MQ=255)
cGGGCACTGTCTTCATCGTGGCACGGCACCGGGTCACCACGGTGGAACAGATCGACGAAAGCACGCGCCGGCTGGCGCAGGCCGACGTCGCCGTACGGGGCGTGATCTTCAACGACTTCACCGGCCACCTGCAc < 1:128425/134‑1 (MQ=255)
gggCACTGTCTTCATCGTGGCACGGCACCGGGTCACCACGGTGGAACAGATCGACGAAAGCACGCGCCGGCTGGCGCAGGCCGACGTCGCCGTACGGGGCGTGATCTTCAACGACTTCACCGGCCACCTGCAcc < 2:669978/134‑1 (MQ=255)
|
TTGCTGCAGCCAGAACTGACCACACTTGTTCAACGCGTTGCCGGCCAGTACGACATGGTGGTGCTTGACGGACCGCCATTGCTGCCGGTGGCAGATGCGCTTGTGCTGGGACGCATGGCGGGCACTGTTTTCATCGTGGCACGGCACCGGGTCACCACGGTGGAACAGATCGACGAAAGCACGCGCCGGCTGGCGCAGGCCGACGTCGCCGTACGGGGCGTGATCTTCAACGACTTCACCGGCCACCTGCACC > AM260479/2016373‑2016625
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
BRESEQ :: bam2aln output
GGGCCAAGCGAGCTGTTGCTGCAGCCAGAACTGACCACACTTGTTCAACGCGTTGCCGGCCAGTACGACATGGTGGTGCTTGACGGACCGCCATTGCTGCCGGTGGCAGATGCGCTTGTGCTGGGACGCATGGCGGGCACTGTTTTCATCGTGGCACGGCACCGGGTCACCACGGTGGAACAGATCGACGAAAGCACGCGCCGGCTGGCGCAGGCCGACGTCGCCGTACGGGGCGTGATCTTCAACGACTTCACCGGCCACCTGCACCGCTACAGCTATGCCTACGGTGCC > AM260479/2016358‑2016648
|
GGGCCAAGCGAGCTGTTGCTGCAGCCAGAACTGACCACACTTGTTCAACGCGTTGCCGGCCAGTACGACATGGTGGTGCTTGACGGACCGCCATTGCTGCCGGTGGCAGATGCGCTTGTGCTGGGACGCATGGCGGGCACTGTCTTCATC < GWNJ‑0478:712:GW2002102894th:2:1208:20694:50752/150‑1 (MQ=60)
ttttttttcaagcagaagacggcatacgaggttagcgagtgtctcgtgggctcggagatgtgtataagagacaGGTGCTTGACGGACCGCCATTGCTGCCGGTGGCAGATGCGCTTGTGCTGGGACGCATGGCGGGCACTGTCTTCATC < GWNJ‑0478:712:GW2002102894th:2:1214:14422:97990/76‑1 (MQ=60)
ttttttttcaagcagaagacggcatacgagattagcgagtgtctcgtgggctcggagatgtgtataagagacaGGTGCTTGACGGACCGCCATTGCTGCCGGTGGCAGATGCGCTTGTGCTGGGACGCATGGCGGGCACTGTCTTCATC < GWNJ‑0478:712:GW2002102894th:2:1207:17029:21884/76‑1 (MQ=60)
ataagagacAGCTGTTGCTGCAGCCAGAACTGACCACACTTGTTCAACGCGTTGCCGGCCAGTACGACATGGTGGTGCTTGACGGACCGCCATTGCTGCCGGTGGCAGATGCGCTTGTGCTGGGACGCATGGCGGGCACTGTCTTCATC < GWNJ‑0478:712:GW2002102894th:2:1110:20284:30213/140‑1 (MQ=60)
gagatgtgtataagagacagCACTTGTTCAACGCGTTGCCGGCCAGTACGACATGGTGGTGCTTGACGGACCGCCATTGCTGCCGGTGGCAGATGCGCTTGTGCTGGGACGCATGGCGGGCACTGTCTTCATCGTGGCACGGCACCGGGT < GWNJ‑0478:712:GW2002102894th:2:1203:13726:88764/130‑1 (MQ=60)
gtgggctcggagatgtgtataagagacaGTTGCCGGCCAGTACGACATGGTGGTGCTTGACGGACCGCCATTGCTGCCGGTGGCAGATGCGCTTGTGCTGGGACGCATGGCGGGCACTGTCTTCATCGTGGCACGGCACCGGGTCACCAC < GWNJ‑0478:712:GW2002102894th:2:1102:5262:100010/122‑1 (MQ=60)
agcgagtgtctcgtgagctcggagatgtgtataagagacaGGTGGTGCTTGACGGACCGCCATTGCTGCCGGTGGCAGATGCGCTTGTGCTGGGACGCATGGCGGGCACTGTCTTCATCGTGGCACGGCACCGGGTCACCACGGTGGAAC < GWNJ‑0478:712:GW2002102894th:2:2110:18398:39214/110‑1 (MQ=60)
gtcttgacgatggctgttttttttttcaagcagaagacggcatacgagtttagcgagtgtctcgtgggctcggagatgtgtataagagacaGGACGCATGGCGGGCACTGTCTTCATCGTGGCACGGCACCGGGTCACCACGGTGGAAC < GWNJ‑0478:712:GW2002102894th:2:1112:18827:61802/58‑1 (MQ=60)
GACCACACTTGTTCAACGCGTTGCCGGCCAGTACGACATGGTGGTGCTTGACGGACCGCCATTGCTGCCGGTGGCAGATGCGCTTGTGCTGGGACGCATGGCGGGCACTGTCTTCATCGTGGCACGGCACCGGGTCACCACGGTGGAAC < GWNJ‑0478:712:GW2002102894th:2:1201:3369:2909/149‑1 (MQ=60)
ACCACACTTGTTCAACGCGTTGCCGGCCAGTACGACATGGTGGTGCTTGACGGACCGCCATTGCTGCCGGTGGCAGATGCGCTTGTGCTGGGACGCATGGCGGGCACTGTCTTCATCGTGGCACGGCACCGGGTCACCACGGTGGAACAG < GWNJ‑0478:712:GW2002102894th:2:1113:17819:29305/150‑1 (MQ=60)
cgtgggctcggagatgtgtataagagacagACGACATGGTGGTGCTTGACGGACCGCCATTGCTGCCGGTGGCAGATGCGCTTGTGCTGGGACGCATGGCGGGCACTGTCTTCATCGTGGCACGGCACCGGGTCACCACGGTGGAAC < GWNJ‑0478:712:GW2002102894th:2:1203:15792:100639/117‑1 (MQ=60)
CACTTGTTCAACGCGTTGCCGGCCAGTACGACATGGTGGTGCTTGACGGACCGCCATTGCTGCCGGTGGCAGATGCGCTTGTGCTGGGACGCATGGCGGGCACTGTCTTCATCGTGGCACGGCACCGGGTCACCACGGTGGAAC > GWNJ‑0478:712:GW2002102894th:2:2106:2266:6434/1‑144 (MQ=60)
ACGCGTTGCCGGCCAGTACGACATGGTGGTGCTTGACGGACCGCCATTGCTGCCGGTGGCAGATGCGCTTGTGCTGGGACGCATGGCGGGCACTGTCTTCATCGTGGCACGGCACCGGGTCACCACGGTGGAACAGATCGACGAAAGCAC > GWNJ‑0478:712:GW2002102894th:2:1108:13411:70298/1‑150 (MQ=60)
GTACGACATGGTGGTGCTTGACGGACCGCCATTGCTGCCGGTGGCAGATGCGCTTGCGCTGGGACGCATGGCGGGCACTGTCTTCATCtgtctcttatacacatctccgagcccacgagacactcgctaatctcgtatgccgtcttctgc > GWNJ‑0478:712:GW2002102894th:2:2212:2148:77502/1‑88 (MQ=60)
cggagatgtgtataagagacagGCCATTGCTGCCGGTGGCAGATGCGCTTGTGCTGGGACGCATGGCGGGCACTGTCTTCATCGTGGCACGGCACCGGGTCACCACGGTGGAACAGATCGACGAAAGCACGCGCCGGCTGGCGCAGGCC < GWNJ‑0478:712:GW2002102894th:2:1106:6134:70772/127‑1 (MQ=60)
ACATGGTGGTGCTTGACGGACCGCCATTGCTGCCGGTGGCAGATGCGCTTGTGCTGGGACGCATGGCGGGCACTGTCTTCATCGTGGCACGGCACCGGGTCctgtctcttatacacatctccgagcccacgagacactcgctaatctcgt > GWNJ‑0478:712:GW2002102894th:2:1101:5843:40458/1‑101 (MQ=60)
CCATTGCTGCCGGTGGCAGATGCGCTTGTGCTGGGACGCATGGCGGGCACTGTCTTCATCcctgtctcttatacacatctccgagcccacgagacactcgctaatctcgtatgccgtcttctgcttgaaaaaaaaaaaaaaaaaccaca > GWNJ‑0478:712:GW2002102894th:2:2104:2602:45786/1‑60 (MQ=60)
CCATTGCTGCCGGTGGCAGATGCGCTTGTGCTGGGACGCATGGCGGGCACTGTCTTCATCGTGGCACGGCACCGGGTCACCACGGTGGAACAGATCGACGAAAGCACGCGCCGGCTGGCGCAGGCCGACGTCGCCGTACGGGGCGTGATC > GWNJ‑0478:712:GW2002102894th:2:2114:20453:41998/1‑150 (MQ=60)
GTGCTGGGACGCATGGCGGGCACTGTCTTCATCGTGGCACGGCACCtgtctcttatacacatctccgagcccacgagacactcgctaatctcgtatgccgtcttctgcttgaaaaaaaaaaatgctaaaattatgaccaaaaaagtctgt > GWNJ‑0478:712:GW2002102894th:2:2107:6344:75633/1‑46 (MQ=60)
GCGGGCACTGTCTTCATCGTGGCACGGCACCGGGTCACCACGGTGGAACAGATCGACGAAAGCACGCGCCGGCTGGCGCAGGCCGACGTCGCCGTACGGGGCGTGATCTTCAACGACTTCACCGGCCACCTGCACCGCTACAGCTATGCC < GWNJ‑0478:712:GW2002102894th:2:1108:16622:85087/150‑1 (MQ=60)
GTCTTCATCGTGGCACGGCACCGGGTCACCACGGTGGAACctgtctcttatacacatctccgagcccacgagacactcgctaatctcgtatgccgtcttctgcttgaaaaaaaaaaagataaaaatacagcacaatacacacagactta > GWNJ‑0478:712:GW2002102894th:2:2213:8611:54556/1‑40 (MQ=60)
GTCTTCATCGTGGCACGGCACCGGGTCACCACGGTGGAACAGATCGACGAAAGCACGCGCCGGCTGGCGCAGGCCGACGTCGCCGTACGGGGCGTGATCTTCAACGACTTCACCGGCCACCTGCACCGCTACAGCTATGCCTACGGTGCC > GWNJ‑0478:712:GW2002102894th:2:2210:17048:24397/1‑150 (MQ=60)
|
GGGCCAAGCGAGCTGTTGCTGCAGCCAGAACTGACCACACTTGTTCAACGCGTTGCCGGCCAGTACGACATGGTGGTGCTTGACGGACCGCCATTGCTGCCGGTGGCAGATGCGCTTGTGCTGGGACGCATGGCGGGCACTGTTTTCATCGTGGCACGGCACCGGGTCACCACGGTGGAACAGATCGACGAAAGCACGCGCCGGCTGGCGCAGGCCGACGTCGCCGTACGGGGCGTGATCTTCAACGACTTCACCGGCCACCTGCACCGCTACAGCTATGCCTACGGTGCC > AM260479/2016358‑2016648
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
