Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A0 F0 I1 R1
|
155 |
25.8 |
2285122 |
88.0% |
2010907 |
104.3 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
AM260479 |
2,579,243 |
A→G |
I111T (ATT→ACT) |
h16_A2374 ← |
Metallopeptidase, M23B subfamily |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | AM260479 | 2,579,243 | 0 | A | G | 100.0%
| 71.1
/ NA
| 20 | I111T (ATT→ACT) | h16_A2374 | Metallopeptidase, M23B subfamily |
| Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (11/9); total (11/9) |
GTGGCGGCCGGGCGGGCCTGGTCGATCGGCTGCGCCTGCACCGTGCCCGGGGCCACCGGCATGGTGGCCACGCCGGGCGCAATGTTGACGTCGGCACCCGGCGGCACGATGCGCAGCAGCTGGCCGACCTCGATCTGGTTCACATTGGTCAGGTTGTTCCACGTTGCCACATCGCGATACGACTGGCCGTTCTCCAGCGCGATTCG > AM260479/2579162‑2579367
|
gTGGCGGCCTGGCGGGCCTGGTCGATCGGCTGCGCCTGCACCGTGCCCGGCGCCACCGGCATGGTGGCCACGCCGGGCGCAGTGTTGACGTCGGCACCCGGCGGCACGATGCGCAGCAGCTGGcc < 1:421878/125‑1 (MQ=255)
gTGGCGGCCGGGCGGGCCTGGTCGATCGGCTGCGCCTGCACCGTGCCCGGCGCCACCGGCATGGTGGCCACGCCGGGCGCAGTGTTGACGTCGGCACCCGGCGGCACGATGCGCAGCAGCTGGcc > 2:421878/1‑125 (MQ=255)
cgggcCTGGTCGATCGGCTGCGCCTGCACCGTGCCCGGCGCCACCGGCATGGTGGCCACGCCGGGCGCAGTGTTGACGTCGGCACCCGGCGGCACGATGCGCAGCAGCTGGCCGACCTCGATCTGGTTCACAtt > 1:945710/1‑134 (MQ=255)
tCGATCGGCTGCGCCTGCACCGTGCCCGGCGCCACCGGCATGGTGGCCACGCCGGGCGCAGTGTTGACGTCGGCACCCGGCGGCACGATGCGCAGCAGCTGGCCGACCTCGATCTGGTTCACATTGGTCAGgtt < 2:286731/134‑1 (MQ=255)
cccGGCGCCACCGGCATGGTGGCCACGCCGGGCGCAGTGTTGACGTCGGCACCCGGCGGCACGATGCGCAGCAGCTGGCCGACCTCGATCTGGTTCACATTGGTCAGGTTGTTCCAc < 2:559020/117‑1 (MQ=255)
cccGGCGCCACCGGCATGGTGGCCACGCCGGGCGCAGTGTTGACGTCGGCACCCGGCGGCACGATGCGCAGCAGCTGGCCGACCTCGATCTGGTTCACATTGGTCAGGTTGTTCCAc > 1:559020/1‑117 (MQ=255)
ccGGCGCCACCGGCATGGTGGCCACGCCGGGCGCAGTGTTGACGTCGGCACCCGGCGGCACGCTGCGCAGCAGCTGGCCGACCTCGATCTGGTTCACATTGGTCAGGTTGTTCCACGTTGCCACATCGCGATAc > 1:251290/1‑134 (MQ=255)
ccGGCGCCACCGGCATGGTGGCCACGCCGGGCGCAGTGTTGACGTCGGCACCCGGCGGCACGATGCGCAGCAGCTGGCCGACCTCGATCTGGTTCACATTGGTCAGGTTGTTCCACGTTGCCACATCGCGATAc > 2:472988/1‑134 (MQ=255)
ccGGCGCCACCGGCATGGTGGCCACGCCGGGCGCAGTGTTGACGTCGGCACCCGGCGGCACGATGCGCAGCAGCTGGCCGACCTCGATCTGGTTCACATTGGTCAGGTTGTTCCACGTTGCCACATCGCGATAc < 2:251290/134‑1 (MQ=255)
cggcgCCACCGGCATGGTGGCCACGCCGGGCGCAGTGTTGACGTCGGCACCCGGCGGCACGATGCGCAGCAGCTGGCCGACCTc < 1:255206/80‑1 (MQ=255)
cggcgCCACCGGCATGGTGGCCACGCCGGGCGCAGTGTTGACGTCGGCACCCGGCGGCACGATGCGCAGCAGCTGGCCGACCTc > 2:255206/5‑84 (MQ=255)
cggcgCCACCGGCATGGTGGCCACGCCGGGCGCAGTGTTGACGTCGGCACCCGGCGGCACGATGCGCAGCAGCTGGCCGACCTCGATCTGGTTCACATTGGTCAGGTTGTTCCACGTTGCCACATCGCGATAc > 1:948709/5‑133 (MQ=255)
cggcgCCACCGGCATGGTGGCCACGCCGGGCGCAGTGTTGACGTCGGCACCCGGCGGCACGATGCGCAGCAGCTGGCCGACCTCGATCTGGTTCACATTGGTCAGGTTGTTCCACGTTGCCACATCGCGATAc > 2:335618/5‑133 (MQ=255)
tggtggCCACGCCGGGCGCAGTGTTGACGTCGGCACCCGGCGGCACGATGCGCAGCAGCTGGCCGACCTCGATCTGGTTCACATTGGTCAGGTTGTTCCACGTTGCCACATCGCGATACGACTGGCCGTTCTcc < 2:1133655/134‑1 (MQ=255)
tggtggCCACGCCGGGCGCAGTGTTGACGTCGGCACCCGGCGGCACGATGCGCAGCAGCTGGCCGACCTCGATCTGGTTCACATTGGTCAGGTTGTTCCACGTTGCCACATCGCGATACGACTGGCCGTTCTcc < 1:472988/134‑1 (MQ=255)
gCCGGGCGCAGTGTTGACGTCGGCACCCGGCGGCACGATGCGCAGCAGCTGGCCGACCTCGATCTGGTTCACAt < 1:889888/74‑1 (MQ=255)
gCCGGGCGCAGTGTTGACGTCGGCACCCGGCGGCACGATGCGCAGCAGCTGGCCGACCTCGATCTGGTTCACAt > 2:889888/1‑74 (MQ=255)
ccGGGCGCAGTGTTGACGTCGGCACCCGGCGGCACGATGCGCAGCAGCTGGCCGACCTCGATCTGGTTCACATTGGTCAGGTTGTTCCACGTTGCCACATCGCGATACGACTGGCCGTTCTCCAGCGCGATTCg > 2:914527/1‑134 (MQ=255)
gggCGCAGTGTTGACGTCGGCACCCGGCGGCACGATGCGCAGCAGCTGGCCGACCt > 1:1120351/1‑56 (MQ=255)
gggCGCAGTGTTGACGTCGGCACCCGGCGGCACGATGCGCAGCAGCTGGCCGACCt < 2:1120351/56‑1 (MQ=255)
|
GTGGCGGCCGGGCGGGCCTGGTCGATCGGCTGCGCCTGCACCGTGCCCGGGGCCACCGGCATGGTGGCCACGCCGGGCGCAATGTTGACGTCGGCACCCGGCGGCACGATGCGCAGCAGCTGGCCGACCTCGATCTGGTTCACATTGGTCAGGTTGTTCCACGTTGCCACATCGCGATACGACTGGCCGTTCTCCAGCGCGATTCG > AM260479/2579162‑2579367
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
BRESEQ :: bam2aln output
AGGCACCGGCGTGGCGGCCGGGCGGGCCTGGTCGATCGGCTGCGCCTGCACCGTGCCCGGGGCCACCGGCATGGTGGCCACGCCGGGCGCAATGTTGACGTCGGCACCCGGCGGCACGATGCGCAGCAGCTGGCCGACCTCGATCTGGTTCACATTGGTCAGGTTGTTCCACGTTGCCACATCGCGATACGACTGGCCGTTCTCCAGCGCGATTCGGTAAAGGGTATCGCCTCGCTTGACC > AM260479/2579152‑2579392
|
AGGCACCGGCGTGGCGGCCTGGCGGGCCTGGTCGATCGGCTGCGCCTGCACCGTGCCCGGCGCCACCGGCATGGTGGCCACGCCGGGCGCAGTGTTGACGTCGGCACCCGGCGGCACGATGCGCAGCAGCTGGCCGACCTCGATCTGGTT < GWNJ‑0478:712:GW2002102894th:2:1209:5147:33179/150‑1 (MQ=60)
agcgagtgtctcgtgggctcggagatgtgtataagagacagcgtcaacactgcGCCCGGCGCCACCGGCATGGTGGCCACGCCGGGCGCAGTGTTGACGTCGGCACCCGGCGGCACGATGCGCAGCAGCTGGCCGACCTCGATCTGGTTC < GWNJ‑0478:712:GW2002102894th:2:2108:6824:35504/97‑1 (MQ=60)
GCACCGGCGTGGCGGCCGGGCGGGCCTGGTCGATCGGCTGCGCCTGCACCGTGCCCGGCGCCACCGGCATGGTGGCCACGCCGGGCGCAGTGTTGACGctgtctcttatacacatctccgagcccacgagacactcgctaatctcgtatg > GWNJ‑0478:712:GW2002102894th:2:2201:6713:69732/1‑98 (MQ=60)
gtgtctcgtgggctcggagatgtgtataagagacaGCGCCTGCACCGTGCCCGGCGCCACCGGCATGGTGGCCACGCCGGGCGCAGTGTTGACGTCGGCACCCGGCGGCACGATGCGCAGCAGCTGGCCGACCTCGATCTGGTTCACATT < GWNJ‑0478:712:GW2002102894th:2:1114:13198:52380/115‑1 (MQ=60)
GGCGTGNCGGCCGGGCGGGCCTGGTCGATCGGCTGCGCCTGCACCGTGCCCGGCGCCACCGGCATGGTGGCCACGCCGGGCGCAGTGTTGACGTCGGCACCCGGCGGCACGATGCGCAGCAGCTGGCCGACCTCGATCTGGTTCACATTG > GWNJ‑0478:712:GW2002102894th:2:2209:21318:61970/1‑150 (MQ=60)
agattagcgagtgtctcgtgggctcggagatgtgtataagagacaggctgcgcatcgtgcCGCCGGGCGCAGTGTTGACGTCGGCACCCGGCGGCACGATGCGCAGCAGCTGGCCGACCTCGATCTGGTTCACATTGGTCAGGTTGTTCC < GWNJ‑0478:712:GW2002102894th:2:2202:6310:24260/90‑1 (MQ=60)
gacgtcaacactgcGCCCGGCGCCACCGGCATGGTGGCCACGCCGGGCGCAGTGTTGACGTCGGCACCCGGCGGCACGATGCGCAGCAGCTGGCCGACCTCGATCTGGTTCACATTGGTCAGGTTGTTCCACGTTGCCACATCGCGActg > GWNJ‑0478:712:GW2002102894th:2:1216:5234:20242/15‑147 (MQ=60)
GCGCCTGCACCGTGCCCGGCGCCACCGGCATGGTGGCCACGCCGGGCGCAGTGTTGACGTCGGCACCCGGCGGCACGCTGCGCAGCAGCTGGCCGACCTCGATCTGGTTCACATTGGTCAGGTTGTTCCACGTTGCCACATCGCGATACG > GWNJ‑0478:712:GW2002102894th:2:1114:18460:33005/1‑150 (MQ=60)
CGCCTGCACCGTGCCCGGCGCCACCGGCATGGTGGCCACGCCGGGCGCAGTGTTGACGTCGGCACCCGGCGGCACGATGCGCAGCAGCTGGCCGACCTCGATCTGGTTCACATTGGTCAGGTTGTTCCACGTTGCCACATCGCGATACG > GWNJ‑0478:712:GW2002102894th:2:2209:18902:78344/1‑149 (MQ=60)
atcgaccaggcccgcccggccGCCACGCCGGGCGCAGTGTTGACGTCGGCACCCGGCGGCACGATGCGCAGCAGCTGGCCGACCTCGATCTGGTTCACATTGGTCAGGTTGTTCCACGTTGCCACATCGCGATACGACTGGCCGTTCTCC < GWNJ‑0478:712:GW2002102894th:2:2206:6323:90824/129‑1 (MQ=60)
gcgcatcgtgcCGCCGGGCGCAGTGTTGACGTCGGCACCCGGCGGCACGATGCGCAGCAGCTGGCCGACCTCGATCTGGTTCACATctgtctcttatacacatctccgagcccacgagacactcgctaatctcgtatgccgtcttctgct > GWNJ‑0478:712:GW2002102894th:2:2215:1754:92999/12‑86 (MQ=60)
ATGGTGGCCACGCCGGGCGCAGTGTTGACGTCGGCACCCGGCGGCACGATGCGCAGCAGCTGGCCGACCTCGATCTGGTTCACATTGGTCAGGTTGTTCCACGTTGCCACATCGCGATACGACTGGCCGTTCTCCAGCGCGATTCGGTAA < GWNJ‑0478:712:GW2002102894th:2:1210:19735:100854/150‑1 (MQ=60)
ACGCCGGGCGCAGTGTTGACGTCGGCACCCGGCGGCACGATGCGCAGCAGCTGGCCGACCTCGATCTGGTTCACATTGGTCAGGTTGTTCCACGTTGCCACATCGCGATACGACTGGCCGTTCTCCAGCGCGATTCGGTAAAGGGTATCG > GWNJ‑0478:712:GW2002102894th:2:1215:16646:67533/1‑150 (MQ=60)
ACGCCGGGCGCAGTGTTGACGTCGGCACCCGGCGGCACGATGCGCAGCAGCTGGCCGACCTCGATCTGGTTCACATTGGTCAGGTTGTTCCACGTTGCCACATCGCGATACGACTGGCCGTTCTCCAGCGCGATTCGGTAAAGGGTATCG > GWNJ‑0478:712:GW2002102894th:2:1214:15493:39602/1‑150 (MQ=60)
GGGCGCAGTGTTGACGTCGGCACCCGGCGGCACGATGCGCAGCAGCTGGCCGACCTCGATCTGGTTCACATTGGTCAGGTTGTTCCACGTTGCCACATCGCGATACGACctgtctcttatacacatctccgagcccacgagacactcgct > GWNJ‑0478:712:GW2002102894th:2:1101:14224:93579/1‑109 (MQ=60)
GTGTTGACGTCGGCACCCGGCGGCACGATGCGCAGCAGCTGGCCGACCTCGATCTGGTTCACATTGGTCAGGTTGTTCCACGTTGCCACATCGCGATACGACTGGCCGTTCTCCctgtctcttatacacatctccgagcccacgagacac > GWNJ‑0478:712:GW2002102894th:2:1110:18013:66017/1‑114 (MQ=60)
GTGTTGACGTCGGCACCCGGCGGCACGATGCGCAGCAGCTGGCCGACCTCGATCTGGTTCACATTGGTCAGGTTGTTCCACGTTGCCACATCGCGATACGACTGGCCGTTCTCCAGCGCGATTCGGTAAAGGGTctgtctcttatacac > GWNJ‑0478:712:GW2002102894th:2:2115:20492:74592/1‑134 (MQ=60)
GTGTTGACGTCGGCACCCGGCGGCACGATGCGCAGCAGCTGGCCGACCTCGATCTGGTTCACATTGGTCAGGTTGTTCCACGTTGCCACATCGCGATACGACTGGCCGTTCTCCAGCGCGATTCGGTAAAGGGTATCGCCTCGCTTGACC > GWNJ‑0478:712:GW2002102894th:2:2214:7825:40502/1‑150 (MQ=60)
|
AGGCACCGGCGTGGCGGCCGGGCGGGCCTGGTCGATCGGCTGCGCCTGCACCGTGCCCGGGGCCACCGGCATGGTGGCCACGCCGGGCGCAATGTTGACGTCGGCACCCGGCGGCACGATGCGCAGCAGCTGGCCGACCTCGATCTGGTTCACATTGGTCAGGTTGTTCCACGTTGCCACATCGCGATACGACTGGCCGTTCTCCAGCGCGATTCGGTAAAGGGTATCGCCTCGCTTGACC > AM260479/2579152‑2579392
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
