Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F1 I1 R1
|
152 |
24.9 |
2083062 |
91.6% |
1908084 |
109.2 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
AM260479 |
2,579,243 |
A→G |
I111T (ATT→ACT) |
h16_A2374 ← |
Metallopeptidase, M23B subfamily |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | AM260479 | 2,579,243 | 0 | A | G | 100.0%
| 33.5
/ NA
| 10 | I111T (ATT→ACT) | h16_A2374 | Metallopeptidase, M23B subfamily |
| Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (4/6); total (4/6) |
CGGCCGCGGCCGGAGGCACCGGCGTGGCGGCCGGGCGGGCCTGGTCGATCGGCTGCGCCTGCACCGTGCCCGGGGCCACCGGCATGGTGGCCACGCCGGGCGCAATGTTGACGTCGGCACCCGGCGGCACGATGCGCAGCAGCTGGCCGACCTCGATCTGGTTCACATTGGTCAGGTTGTTCCACGTTGCCACATCGCGATACGACTGGCCGTTCTCCAGCG > AM260479/2579139‑2579360
|
cggccgcggccgGAGGCACCGGCGTGGCGGCCGGGCGGGCCTGGTCGATCGGCTGCGCCTGCACCGTGCCCGGCGCCACCGGCATGGTGGCCACGCCGGGCGCAGTGTTGACGTCGGCACCCGGCGGCACGATg > 1:1027096/1‑134 (MQ=255)
ggccgGAGGCACCGGCGTGGCGGCCGGGCGGGCCTGGTCGATCGGCTGCGCCTGCACCGTGCCCGGCGCCACCGGCATGGTGGCCACGCCGGGCGCAgtgt > 1:806833/1‑101 (MQ=255)
ggccgGAGGCACCGGCGTGGCGGCCGGGCGGGCCTGGTCGATCGGCTGCGCCTGCACCGTGCCCGGCGCCACCGGCATGGTGGCCACGCCGGGCGCAgtgt < 2:806833/101‑1 (MQ=255)
tGGCGGCCGGGCGGGCCTGGTCGATCGGCTGCGCCTGCACCGTGCCCGGCGCCACCGGCATGGTGGCCACGCCGGGCGCAGTGTTGACGTCGGCACCCGGCGGCACGATGCGCAGCAGCTGGCCGACCTCGATc < 1:665275/134‑1 (MQ=255)
gcgggcCTGGTCGATCGGCTGCGCCTGCACCGTGCCCGGCGCCACCGGCATGGTGGCCACGCCGGGCGCAGTGTTGACGTCGGCACCCGGCGGCACGATGCGCAGCAGCTGGCCGACCTCGATCTGGTTCACAt < 2:1027096/134‑1 (MQ=255)
gATCGGCTGCGCCTGCACCGTGCCCGGCGCCACCGGCATGGTGGCCACGCCGGGCGCAGTGTTGACGTCGGCACCCGGCGGCACGATGCGCAGCAGCTGGCCGACCTCGATCTGGTTCACATTGGTCAGgttgt < 2:547340/134‑1 (MQ=255)
aTCGGCTGCGCCTGCACCGTGCCCGGCGCCACCGGCATGGTGGCCACGCCGGGCGCAGTGTTGACGTCGGCACCCGGCGGCACGATGCGCAGCAGCTGGCCGACCTCGATCTGGTTCACATTGGTCAGgttgtt > 2:172015/1‑134 (MQ=255)
gCCACGCCGGGCGCAGTGTTGACGTCGGCACCCGGCGGCACGATGCGCAGCAGCTGGCCGACCTCGATCTGGTTCACATTGGTCAGGTTGTTCCACGTTGCCACATCGCGATACGACTGGCCGTTCTCCAgcg < 2:715961/133‑1 (MQ=255)
ccGGGCGCAGTGTTGACGTCGGCACCCGGCGGCACGATGCGCagcagc > 1:614461/1‑48 (MQ=255)
ccGGGCGCAGTGTTGACGTCGGCACCCGGCGGCACGATGCGCagcagc < 2:614461/48‑1 (MQ=255)
gggCGCAGTGTTGACGTCGGCACCCGGCGGCACGATGCGCAGCAGCTGGCCGACCTCGATCTGGTTCACATTGGTCAGGTTGTTCCACGTTGCCACATCGCGATACGACTGGCCGTTCTCCAgcg < 1:104477/125‑1 (MQ=255)
gggCGCAGTGTTGACGTCGGCACCCGGCGGCACGATGCGCAGCAGCTGGCCGACCTCGATCTGGTTCACATTGGTCAGGTTGTTCCACGTTGCCACATCGCGATACGACTGGCCGTTCTCCAgcg > 2:104477/1‑125 (MQ=255)
|
CGGCCGCGGCCGGAGGCACCGGCGTGGCGGCCGGGCGGGCCTGGTCGATCGGCTGCGCCTGCACCGTGCCCGGGGCCACCGGCATGGTGGCCACGCCGGGCGCAATGTTGACGTCGGCACCCGGCGGCACGATGCGCAGCAGCTGGCCGACCTCGATCTGGTTCACATTGGTCAGGTTGTTCCACGTTGCCACATCGCGATACGACTGGCCGTTCTCCAGCG > AM260479/2579139‑2579360
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
BRESEQ :: bam2aln output
CCGCAGGCGCCGAAGCGGCCGCGGCCGGAGGCACCGGCGTGGCGGCCGGGCGGGCCTGGTCGATCGGCTGCGCCTGCACCGTGCCCGG‑‑‑GGCCACCGGCAT‑‑‑GGTGGCCACGCCGGGCGCAATGTTGACGTCGGCACCCGGCGGCACGATGCGCAGCAGCTGGCCGACCTCGATCTGGTTCACATTGGTCAGGTTGTTCCACGTTGCCACATCGCGATACGACTGGCCGTTCTCCAGCGCGATTCGGTAAAGGGTATCGCCTCGCTTGACC > AM260479/2579124‑2579392
|
gtGCAGGCGCCGAAGCGGCCGCGGCCGGAGGCACCGGCGTGGCGGCCGGGCGGGCCTGGTCGATCGGCTGCGCCTGCACCGTGCCCGG‑‑‑CGCCACCGGCAT‑‑‑GGTGGCCACGCCGGGCGCAGTGTTGACGTCGGCACCCGGCGGCACGATGC > GWNJ‑0478:712:GW2002102894th:2:2216:4544:24640/3‑150 (MQ=60)
CGCCGAAGCGGCCGCGGCCGGAGGCACCGGCGTGGCGGCCGGGCGGGCCTGGTCGATCGGCTGCGCCTGCACCGTGCCCGG‑‑‑CGCCACCGGCAT‑‑‑GGTGGCCACGCCGGGCGCAGTGTTGACGTCGGCACCCGctgtctcttatacacatct > GWNJ‑0478:712:GW2002102894th:2:2202:6324:2179/1‑131 (MQ=60)
GTGGCGGCCGGGCGGGCCTGGTCGATCGGCTGCGCCTGCACCGTGCCCGG‑‑‑CGCCACCGGCAT‑‑‑GGTGGCCACGCCGGGCGCAGTGTTGACGTCGGCACCCGGCGGCACGATGCGCAGCAGCTGGCCGACCTCGATCTGGTTCACATTGGTC < GWNJ‑0478:712:GW2002102894th:2:2111:4127:55893/150‑1 (MQ=60)
GGCCTGGTCGATCGGCTGCGCCTGCACCGTGCCCGG‑‑‑CGCCACCGGCAT‑‑‑GGTGGCCACGCCGGGCGCAGTGTTGACGTCctgtctcttatacacatctccgagcccacgagacggagctacatctcgtatgccgtcttctgcttgaaaaa > GWNJ‑0478:712:GW2002102894th:2:2210:20051:27738/1‑78 (MQ=60)
ATCGGCTGCGCCTGCACCGTGCCCGGCGTGGCCAC‑‑‑CATGCCGGTGG‑‑‑CGCCGGGCGCAGTGTTGACGTCGGCACCCGGCGGCACGATGCGCAGCAGCTGGCCGACCTCGATCTGGTTCACATTGGTCctgtctcttatgcacatcttccg > GWNJ‑0478:712:GW2002102894th:2:1204:1408:76608/1‑126 (MQ=60)
GGCTGCGCCTGCACCGTGCCCGG‑‑‑CGCCACCGGCAT‑‑‑GGTGGCCACGCCGGGCGCAGTGTTGACGTCGGCACCCtgtctcttatacacatctccgagcccacgagacggagctacaactcgtatgccgtcttctgcttgaaaaaaaaaaac > GWNJ‑0478:712:GW2002102894th:2:2115:12365:52769/1‑72 (MQ=60)
GGCAT‑‑‑GGTGGCCACGCCGGGCGCAGTGTTGACGTCGGCACCCGGCGGCACGATGCGCAGCAGCTGGCCGACCTCGATCctgtctcttatacacatccccgagcccacgagacggagctacatctcgtatgccgtcttctgcttgaaaaa > GWNJ‑0478:712:GW2002102894th:2:2106:20309:37655/1‑78 (MQ=60)
cgtgcCGCCGGGCGCAGTGTTGACGTCGGCACCCGGCGGCACGATGCGCAGCAGCTGGCCGACCTCGATCTGGTTCACATTGGTCAGGTTGTTCCACGTTGCCACATCGCGATACGACTGGCCGTTCTCCAGCGCGATTCGGTAAAGGG < GWNJ‑0478:712:GW2002102894th:2:1108:4155:9473/144‑1 (MQ=60)
GGCCACGCCGGGCGCAGTGTTGACGTCGGCACCCGGCGGCACGATGCGCAGCAGCTGGCCGACCTCGATCTGGTTCACATctgtctcttatacacatctccgagcccacgagacggagctacatctcgtatgccgtcttctgcttgaaa > GWNJ‑0478:712:GW2002102894th:2:1109:7469:44341/1‑80 (MQ=60)
GTGTTGNCGTCGGCACCCGGCGGCACGATGCGCAGCAGCTGGCCGACCTCGATCTGGTTCACATTGGTCAGGTTGTTCCACGTTGCCACATCGCGATACGACTGGCCGTTCTCCAGCtgtctcttatacacatctccgagcccacgagac > GWNJ‑0478:712:GW2002102894th:2:1209:21300:63971/1‑117 (MQ=60)
GTGTTGACGTCGGCACCCGGCGGCACGATGCGCAGCAGCTGGCCGACCTCGATCTGGTTCACATTGGTCAGGTTGTTCCACGTTGCCACATCGCGATACGACTGGCCGTTCTCCAGCGCGActgtctcttatacacatctccgagcccac > GWNJ‑0478:712:GW2002102894th:2:2112:2153:68129/1‑121 (MQ=60)
|
CCGCAGGCGCCGAAGCGGCCGCGGCCGGAGGCACCGGCGTGGCGGCCGGGCGGGCCTGGTCGATCGGCTGCGCCTGCACCGTGCCCGG‑‑‑GGCCACCGGCAT‑‑‑GGTGGCCACGCCGGGCGCAATGTTGACGTCGGCACCCGGCGGCACGATGCGCAGCAGCTGGCCGACCTCGATCTGGTTCACATTGGTCAGGTTGTTCCACGTTGCCACATCGCGATACGACTGGCCGTTCTCCAGCGCGATTCGGTAAAGGGTATCGCCTCGCTTGACC > AM260479/2579124‑2579392
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
