Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A0 F0 I1 R1
|
155 |
25.8 |
2285122 |
88.0% |
2010907 |
104.3 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
AM260480 |
1,166,676 |
+G |
intergenic (+150/‑109) |
h16_B1032 → / → h16_B1033 |
conserved hypothetical protein/probable extra‑cytoplasmic solute receptor |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | AM260480 | 1,166,674 | 1 | . | G | 90.9%
| 73.2
/ 0.9
| 22 | intergenic (+148/‑111) | h16_B1032/h16_B1033 | conserved hypothetical protein/probable extra‑cytoplasmic solute receptor |
| Reads supporting (aligned to +/- strand): ref base . (1/1); new base G (10/10); total (11/11) |
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.59e-01 |
ACTGACGGCCCGGCATCCCGGACCCAACATGTGATGGCGAAGGCGGGACCCGCGACGGTGTGGGTCCCGCTCTTTCGCCTTGCTGCCGTGGACCCCCCAGACCTCAATTGCCGTGTGGCCGATACCGCGAGCA‑GGTTTTCCCTCTGTTTGCGCGAATCGACAACAACTTGGCGTGATCGGTCAAGCCGGTTGACGCGTGGCGATCCGAGAATTGAGAAAAGACGGCAATCAAGAGGTACAGACATGAGGAAGCGAA > AM260480/1166542‑1166797
|
aCTGACGGCCCGGCATCCCGGACCCAACATGTGATGGCGAAGGCGGGACCCGCGACGGTGTGGGTCCCGCTCTTTCGCCTTGCTGCCGTGGACCCCCCAGACCTCAATTGCCGTGTGGCCGATACCGCGAGCA‑g < 2:112312/134‑1 (MQ=255)
aCGGCCCGGCATCCCGGACCCAACATGTGATGGCGAAGGCGGGACCCGCGACGGTGTGGGTCCCGCTCTTTCGCCTTGCTGCCGTGGACCCCCCAGACCTCAATTGCCGTGTGGCCGATACCGCGAGCA‑gggtt > 2:744950/1‑131 (MQ=255)
ggACCCAACATGTGATGGCGAAGGCGGGACCCGCGACGGTGTGGGTCCCGCTCTTTCGCCTTGCTGCCGTGGACCCCCCAGACCTCAATTGCCGTGTGGCCGATACCGCGAGCAGGGTTTTCCCTCTGTTTgcg < 1:744950/134‑1 (MQ=255)
aCCCAACATGTGATGGCGAAGGCGGGACCCGCGACGGTGTGGGTCCCGCTCTTTCGCCTTGCTGCCGTGGACCCCCCAGACCTCAATTGCCGTGTGGCCGATACCGCGAGCAGGGTTTTCCCTCTGTTTGcgcg < 2:656933/134‑1 (MQ=255)
aCATGTGATGGCGAAGGCGGGACCCGCGACGGTGTGGGTCCCGCTCTTTCGCCTTGCTGCCGTGGACCCCCCAGACCTCAATTGCCGTGTGGCCGATACCGCGAGCAGGGTTTTCCCTCTGTTTGCGCGAATc > 2:457228/1‑133 (MQ=255)
tgATGGCGAAGGCGGGACCCGCGACGGTGTGGGTCCCGCTCTTTCGCCTTGCTGCCGTGGACCCCCCAGACCTCAATTGCCGTGTGGCCGATACCGCGAGCA‑GGgtttt < 2:956909/109‑2 (MQ=255)
tgATGGCGAAGGCGGGACCCGCGACGGTGTGGGTCCCGCTCTTTCGCCTTGCTGCCGTGGACCCCCCAGACCTCAATTGCCGTGTGGCCGATACCGCGAGCA‑GGgtttt > 1:956909/1‑108 (MQ=255)
aaGGCGGGACCCGCGACGGTGTGGGTCCCGCTCTTTCGCCTTGCTGCCGTGGACCCCCCAGACCTCAATTGCCGTGTGGCCGATACCGCGAGCAGGGTTTTCCCTCTGTTTGCGCGAATCGACAACAACTTGGc > 2:613660/1‑134 (MQ=255)
gtgtGGGTCCCGCTCTTTCGCCTTGCTGCCGTGGACCCCCCAGACCTCAATTGCCGTGTGGCCGATACCGCGAGCAGGGTTTTCCCTCTGtt < 1:659079/92‑1 (MQ=255)
gtgtGGGTCCCGCTCTTTCGCCTTGCTGCCGTGGACCCCCCAGACCTCAATTGCCGTGTGGCCGATACCGCGAGCAGGGTTTTCCCTCTGtt > 2:659079/1‑92 (MQ=255)
ggTCCCGCTCTTTCGCCTTGCTGCCGTGGACCCCCCAGACCTCAATTGCCGTGTGGCCGATACCGCGAGCAGGGTTTTCCCTCTGTTTGCGCGAATCGACAACAACTTGGCGTGATCGGTCAAGCCGGTTGAcg > 1:832704/1‑134 (MQ=255)
ggTCCCGCTCTTTCGCCTTGCTGCCGTGGACCCCCCAGACCTCAATTGCCGTGTGGCCGATACCGCGAGCAGGGTTTTCCCTCTGTTTGCGCGAATCGACAACAACTTGGCGTGATCGGTCAAGCCGGTTGAcg > 1:131830/1‑134 (MQ=255)
tttCGCCTTGCTGCCGTGGACCCCCCAGACCTCAATTGCCGTGTGGCCGATACCGCGAGCAGGGTTTTCCCTCTGTTTGCGCGAATCGACAACAACTTGGCGTGATCGGTCAAGCCGGTTGACGCGTGGCGATc > 1:925730/1‑134 (MQ=255)
tCGCCTTGCTGCCGTGGACCCCCCAGACCTCAATTGCCGTGTGGCCGATACCGCGAGCAGGGTTTTCCCTCTGTTTGCGCGAATCGACAACAACTTGGCGTGATCGGTCAAGCCGGTTGACGCGTGGCGATcc < 2:131830/133‑1 (MQ=255)
tCGCCTTGCTGCCGTGGACCCCCCAGACCTCAATTGCCGTGTGGCCGATACCGCGAGCAGGGTTTTCCCTCTGTTTGCGCGAATCGACAACAACTTGGCGTGATCGGTCAAGCCGGTTGACGCGTGGCGATcc < 2:832704/133‑1 (MQ=255)
ccTCAATTGCCGTGTGGCCGATACCGCGAGCAGGGTTTTCCCTCTGTTTGCGCGAATCGACAACAACTTGGCGTGATCGGTCAAGCCGGTTGACGCGTGGCGATCCGAGAATTGAGAAAAGACGGCAATCAaga < 2:472834/134‑1 (MQ=255)
ggCCGATACCGCGAGCAGGGTTTTCCCTCTGTTTGCGCGAATCGACAACAACTTGGCGTGATCGGTCAAGCCGGTTGACGCGTGGCGATCCGAGAATTGAGAAAAGACGGCaa > 2:1054762/1‑113 (MQ=255)
ggCCGATACCGCGAGCAGGGTTTTCCCTCTGTTTGCGCGAATCGACAACAACTTGGCGTGATCGGTCAAGCCGGTTGACGCGTGGCGATCCGAGAATTGAGAAAAGACGGCaa < 1:1054762/113‑1 (MQ=255)
cGATACCGCGAGCAGGGTTTTCCCTCTGTTTGCGCGAATCGACAACAACTTGGCGTGATCGGTCAAGCCGGTTGACGCGTGGCGATCCGAGAATTGAGAAAAGACGGCAATCAAGAGGTACAGACATGAGGAAg > 1:68076/1‑134 (MQ=255)
aTACCGCGAGCAGGGTTTTCCCTCTGTTTGCGCGAATCGACAACAACTTGGCGTGATCGGTCAAGc < 1:1015871/66‑1 (MQ=255)
aTACCGCGAGCAGGGTTTTCCCTCTGTTTGCGCGAATCGACAACAACTTGGCGTGATCGGTCAAGc > 2:1015871/1‑66 (MQ=255)
ccGCGAGCAGGGTTTTCCCTCTGTTTGCGCGAATCGACAACAACTTGGCGTGATCGGTCAAGCCGGTTGACGCGTGGCGATCCGAGAATTGAGAAAAGACGGCAATCAAGAGGTACAGACATGAGGAAGCGaa < 2:68076/133‑1 (MQ=255)
cgAGCAGGGTTTTCCCTCTGTTTGCGCGAATCGACAACAACTTGGCGTGATCGGTCAAGCCGGTTGACGCGTGGCGATCCGAGAATTGAGAAAAg > 1:378915/1‑95 (MQ=255)
cgAGCAGGGTTTTCCCTCTGTTTGCGCGAATCGACAACAACTTGGCGTGATCGGTCAAGCCGGTTGACGCGTGGCGATCCGAGAATTGAGAAAAg < 2:378915/95‑1 (MQ=255)
|
ACTGACGGCCCGGCATCCCGGACCCAACATGTGATGGCGAAGGCGGGACCCGCGACGGTGTGGGTCCCGCTCTTTCGCCTTGCTGCCGTGGACCCCCCAGACCTCAATTGCCGTGTGGCCGATACCGCGAGCA‑GGTTTTCCCTCTGTTTGCGCGAATCGACAACAACTTGGCGTGATCGGTCAAGCCGGTTGACGCGTGGCGATCCGAGAATTGAGAAAAGACGGCAATCAAGAGGTACAGACATGAGGAAGCGAA > AM260480/1166542‑1166797
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
BRESEQ :: bam2aln output
TTGGAGGAACTGACGGCCCGGCATCCCGGACCCAACATGTGATGGCGAAGGCGGGACCCGCGACGGTGTGGGTCCCGCTCTTTCGCCTTGCTGCCGTGGACCCCCCAGACCTCAATTGCCGTGTGGCCGATACCGCGAGCA‑GGTTTTCCCTCTGTTTGCGCGAATCGACAACAACTTGGCGTGATCGGTCAAGCCGGTTGACGCGTGGCGATCCGAGAATTGAGAAAAGACGGCAATCAAGAGGTACAGACATGAGGAAGCGAACCAATAACCTATG > AM260480/1166534‑1166810
|
ccttgttctttttcttccttcttatttttttttttttttttttcaagcagaagacggcatacgagattagcgagtgtctcgtgggctcggagatgtgtataagagacagCCTCAATTGCCGTGTGGCCGATACCGCGAGCAGGGTTTTCC < GWNJ‑0478:712:GW2002102894th:2:1109:21081:51630/41‑1 (MQ=60)
ggagatgtgtataagagacagCCCGGACCCAACATGTGATGGCGAAGGCGGGACCCGCGACGGTGTGGGTCCCGCTCTTTCGCCTTGCTGCCGTGGACCCCCCAGACCTCAATTGCCGTGTGGCCGATACCGCGAGCAGGGTTTTCCCTC < GWNJ‑0478:712:GW2002102894th:2:1211:13964:45910/129‑1 (MQ=60)
gggctcggagatgtgtataagagacAGGCGGGACCCGCGACGGTGTGGGTCCCGCTCTTTCGCCTTGCTGCCGTGGACCCCCCAGACCTCAATTGCCGTGTGGCCGATACCGCGAGCAGGGTTTTCCCTCTGTTTGCGCGAATCGACAA < GWNJ‑0478:712:GW2002102894th:2:2107:16042:42896/124‑1 (MQ=60)
CCCGGACCCAACATGTGATGGCGAAGGCGGGACCCGCGACGGTGTGGGTCCCGCTCTTTCGCCTTGCTGCCGTGGACCCCCCAGACCTCAATTGCCGTGTGGCCGATACCGCGAGCAGGGTTTTCCCTCTGTTTGCGCGctgtctcttat > GWNJ‑0478:712:GW2002102894th:2:2206:1735:25485/1‑139 (MQ=60)
CGGACCCAACATGTGATGGCGAAGGCGGGACCCGCGACGGTGTGGGTCCCGCTCTTTCGCCTTGCTGCCGTGGACCCCCCAGACCTCAATTGCCGTGTGGCCGATACCGCGAGCAGGGTTTTCCCTCTGTTTGCGCGAATCGACAACAAC < GWNJ‑0478:712:GW2002102894th:2:2110:7548:31337/150‑1 (MQ=60)
ACCCGCGACGGTGTGGGTCCCGCTCTTTCGCCTTGCTGCCGTGGACCCCCCAGACCTCAATTGCCGTGTGGCCGATACCGCGAGCAGGGTTTTCCCTCTGTTTGCGCGAATCGACAACAACTTGGCGTGATCGGTCAAGCCGGTTGACGC > GWNJ‑0478:712:GW2002102894th:2:1109:21111:4812/1‑150 (MQ=60)
ACCCGCGACGGTGTGGGTCCCGCTCTTTCGCCTTGCTGCCGTGGACCCCCCAGACCTCAATTGCCGTGTGGCCGATACCGCGAGCAGGGTTTTCCCTCTGTTTGCGCGAATCGACAACAACTTGGCGTGATCGGTCAAGCCGGTTGACGC > GWNJ‑0478:712:GW2002102894th:2:2114:3268:84950/1‑150 (MQ=60)
ggcatacgagattagcgagtgtctcgtgggctcggagatgtgtataagagacagATTGCCGTGTGGCCGATACCGCGAGCAGGGTTTTCCCTCTGTTTGCGCGAATCGACAACAACTTGGCGTGATCGGTCAAGCCGGTTGACGCGTGGC < GWNJ‑0478:712:GW2002102894th:2:2211:7526:63223/96‑1 (MQ=60)
GTGTGGGTCCCGCTCTTTCGCCTTGCTGCCGTGGACCCCCCAGACCTCAATTGCCGTGTGGCCGATACCGCGAGCAGGGTTTTCCCTCTGTTTGCGCGAATCGACAACAACTTGGCGTGATCGGTCAAGCCGGTTGACGCGTGGCGATCC > GWNJ‑0478:712:GW2002102894th:2:2205:4942:47267/1‑150 (MQ=60)
agattagcgagtgtctcgtgggctcggagatgtgtataagagacagGGCCGATACCGCGAGCAGGGTTTTCCCTCTGTTTGCGCGAATCGACAACAACTTGGCGTGATCGGTCAAGCCGGTTGACGCGTGGCGATCCGAGAATTGAGAAA < GWNJ‑0478:712:GW2002102894th:2:2115:19402:29227/104‑1 (MQ=60)
gagacagCCTCAATTGCCGTGTGGCCGATACCGCGAGCAGGGTTTTCCCTCTGTTTGCGCGAATCGACAACAACTTGGCGTGATCGGTCAAGCCGGTTGACGCGTGGCGATCCGAGAATTGAGAAAAGACGGCAATCAAGAGGTACAGAC < GWNJ‑0478:712:GW2002102894th:2:2212:5931:67599/143‑1 (MQ=60)
CAATTGCCGTGTGGCCGATACCGCGAGCAGGGTTTTCCCTCTGTTTGCGCGAATCGACAACAACTTGGCGTGATCGGTCAAGCCGGTTGACGCGTGGCGATCCGAGAATTGAGAAAAGACGGCAATCAAGAGGTACAGACATGAGGAAGC > GWNJ‑0478:712:GW2002102894th:2:1104:13982:32026/1‑150 (MQ=60)
GTGTGGCCGATACCGCGAGCAGGGTTTTCCCTCTGTTTGCGCGAATCGACAACAACTTGGCGTGATCGGTCAAGCCGGTTGACGCGTGGCGATCCGAGAATTGAGAAAAGACGGCAATCAAGAGGctgtctcttatacacatctccgagc > GWNJ‑0478:712:GW2002102894th:2:1207:17918:91570/1‑125 (MQ=60)
ATACCGCGAGCAGGGTTTTCCCTCTGTTTGCGCGAATCGACAACAACTTGGCGctgtctcttatacacatctccgagcccacgagacactcgctaatctcgtatgccgtcttctgcttgaaaaaaaaaaaaaaaaaactgataaaaaaa > GWNJ‑0478:712:GW2002102894th:2:2105:2945:34043/1‑53 (MQ=60)
|
TTGGAGGAACTGACGGCCCGGCATCCCGGACCCAACATGTGATGGCGAAGGCGGGACCCGCGACGGTGTGGGTCCCGCTCTTTCGCCTTGCTGCCGTGGACCCCCCAGACCTCAATTGCCGTGTGGCCGATACCGCGAGCA‑GGTTTTCCCTCTGTTTGCGCGAATCGACAACAACTTGGCGTGATCGGTCAAGCCGGTTGACGCGTGGCGATCCGAGAATTGAGAAAAGACGGCAATCAAGAGGTACAGACATGAGGAAGCGAACCAATAACCTATG > AM260480/1166534‑1166810
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
