Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F1 I1 R1
|
152 |
24.9 |
2083062 |
91.6% |
1908084 |
109.2 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
AM260480 |
1,166,676 |
+G |
intergenic (+150/‑109) |
h16_B1032 → / → h16_B1033 |
conserved hypothetical protein/probable extra‑cytoplasmic solute receptor |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | AM260480 | 1,166,674 | 1 | . | G | 100.0%
| 64.8
/ NA
| 18 | intergenic (+148/‑111) | h16_B1032/h16_B1033 | conserved hypothetical protein/probable extra‑cytoplasmic solute receptor |
| Reads supporting (aligned to +/- strand): ref base . (0/0); new base G (11/7); total (11/7) |
ACTGACGGCCCGGCATCCCGGACCCAACATGTGATGGCGAAGGCGGGACCCGCGACGGTGTGGGTCCCGCTCTTTCGCCTTGCTGCCGTGGACCCCCCAGACCTCAATTGCCGTGTGGCCGATACCGCGAGCA‑GGTTTTCCCTCTGTTTGCGCGAATCGACAACAACTTGGCGTGATCGGTCAAGCCGGTTGACGCGTGGCGATCCGAGAATTGAGAAAAGACGGCAATCAAGAGGTACAGACATGAGG > AM260480/1166542‑1166790
|
aCTGACGGCCCGGCATCCCGGACCCAACATGTGATGGCGAAGGCGGGACCCGCGACGGTGTGGGTCCCGCTCTTTCGCCTTGCTGCCGTGGACCCCCCAGACCTCAATTGCCGTGTGGCCGATACCGCGAGCA‑g < 1:237024/134‑1 (MQ=255)
cccGGCATCCCGGACCCAACATGTGATGGCGAAGGCGGGACCCGCGACGGTGTGGGTCCCGCTCTTTCGCCTTGCTGCCGTGGACCCCCCAGACCTCAATTGCCGTGTGGCCGATACCGCGAGCAGGGTTTTcc > 1:208711/1‑134 (MQ=255)
cGAAGGCGGGACCCGCGACGGTGTGGGTCCCGCTCTTTCGCCTTGCTGCCGTGGACCCCCCAGACCTCAATTGCCGTGTGGCCGATACCGCGAGCAGGGTTTTCCCTCTGTTTGCGCGAATCGACAACAACTTg > 1:905082/1‑134 (MQ=255)
gCGGGACCCGCGACGGTGTGGGTCCCGCTCTTTCGCCTTGCTGCCGTGGACCCCCCAGACCTCAATTGCCGTGTGGCCGATACCGCGAGCAGGGTTTTCCCTCTGTTTgcg > 1:231476/1‑111 (MQ=255)
gCGGGACCCGCGACGGTGTGGGTCCCGCTCTTTCGCCTTGCTGCCGTGGACCCCCCAGACCTCAATTGCCGTGTGGCCGATACCGCGAGCAGGGTTTTCCCTCTGTTTgcg < 2:231476/111‑1 (MQ=255)
cccGCGACGGTGTGGGTCCCGCTCTTTCGCCTTGCTGCCGTGGACCCCCCAGACCTCAATTGCCGTGTGGCCGATACCGCGAGCAGGGTTTTCCCTCTGTTTGCGCGAATCGACAACAACTTGGCGTGATCGGt > 2:620530/1‑134 (MQ=255)
cGGTGTGGGTCCCGCTCTTTCGCCTTGCTGCCGTGGACCCCCCAGACCTCAATTGCCGTGTGGCCGATACCGCGAGCAGGGTTTTCCCTCTGTTTGCGCGAATCGACAACAACTTGGCGTGATCGGTCAAGCCg > 2:640027/1‑134 (MQ=255)
ctTTCGCCTTGCTGCCGTGGACCCCCCAGACCTCAATTGCCGTGTGGCCGATACCGCGAGCA‑gggtt < 1:1012842/67‑4 (MQ=255)
ctTTCGCCTTGCTGCCGTGGACCCCCCAGACCTCAATTGCCGTGTGGCCGATACCGCGAGCA‑gggtt > 2:1012842/1‑64 (MQ=255)
tttCGCCTTGCTGCCGTGGACCCCCCAGACCTCAATTGCCGTGTGGCCGATACCGCGAGCAGGGTCTTCCCTCTGTTTGCGCGAATCGACAACAACTTGGCGTGATAGGTCAAGCCGGTTGACGCGTGGCGATc > 1:404532/1‑134 (MQ=255)
cGCCTTGCTGCCGTGGACCCCCCAGACCTCAATTGCCGTGTGGCCGATACCGCGAGCAGGGTTTTCCCTCTGTTTGCGCGAATCGACAACAACTTGGCGTGATCGGTCAAGCCGGTTGACGCGTGGCGATCCg < 2:208711/133‑1 (MQ=255)
cTTGCTGCCGTGGACCCCCCAGACCTCAATTGCCGTGTGGCCGATACCGCGAGCAGGGTTTTCCCTCTGttt > 1:381226/1‑72 (MQ=255)
cTTGCTGCCGTGGACCCCCCAGACCTCAATTGCCGTGTGGCCGATACCGCGAGCAGGGTTTTCCCTCTGttt < 2:381226/72‑1 (MQ=255)
cTTGCTGCCGTGGACCCCCCAGACCTCAATTGCCGTGTGGCCGATACCGCGAGCAGGGTTTTCCCTCTGTTTGCGCGAATCGACAACAACTTGGCGTGATCGGTCAAGCCGGTTGACGCGTGGCGATCCGAGaa > 1:776782/1‑134 (MQ=255)
ccAGACCTCTATTGTCGTGTGGCCGATACCGCGAGCAGGGTTTTCCCTCTGTTTGCGCGAATCGACAACAACTTGGCGTGATCGGTCAAGCCGGTTGACGCGTGGCGATCCGAGAATTGAGAAAAGACGGCAAt < 2:404532/134‑1 (MQ=255)
ccAGACCTCAATTGCCGTGTGGCCGATACCGCGAGCAGGGTTTTCCCTCTGTTTGCGCGAATCGACAACAACTTGGCGTGATCGGTCAAGCCGGTTGACGCGTGGCGATCCGAGAATTGAGAAAAGACGGCAAt < 2:776782/134‑1 (MQ=255)
gCCGTGTGGCCGATACCGCGAGCAGGGTTTTCCCTCTGTTTGCGCGAATCGACAACAACTTGGCGTGATCGGTCAAGCCGGTTGACGCGTGGCGATCCGAGAATTGAGAAAAGACGGCAAt < 2:882388/121‑1 (MQ=255)
gCCGTGTGGCCGATACCGCGAGCAGGGTTTTCCCTCTGTTTGCGCGAATCGACAACAACTTGGCGTGATCGGTCAAGCCGGTTGACGCGTGGCGATCCGAGAATTGAGAAAAGACGGCAAt > 1:882388/1‑121 (MQ=255)
ccGTGTGGCCGATACCGCGAGCAGGGTTTTCCCTCTGTTTGCGCGAATCGACAACAACTTGGCGTGATCGGTCAAGCCGGTTGACGCGTGGCGATCCGAGAATTGAGAAAAGACGGCAATCAAGAGGTACAGAc < 1:572917/134‑1 (MQ=255)
ggCCGATACCGCGAGCAGGGTTTTCCCTCTGTTTGCGCGAATCGACAACAACTTGGCGTGATCGGTCAAGCCGGTTGACGCGTGGCGATCCGAGAATTGAAAAAAGACGGCAATCAAGAGGTACAGACATGAg > 1:512417/1‑133 (MQ=255)
gCCGATACCGCGAGCAGGGTTTTCCCTCTGTTTGCGCGAATCGACAACAACTTGGCGTGATCGGTCAAGCCGGTTGACGCGTGGCGATCCGAGAATTGAGAAAAGACGGCAATCAAGAGGTACAGACATGAgg > 1:527221/1‑133 (MQ=255)
|
ACTGACGGCCCGGCATCCCGGACCCAACATGTGATGGCGAAGGCGGGACCCGCGACGGTGTGGGTCCCGCTCTTTCGCCTTGCTGCCGTGGACCCCCCAGACCTCAATTGCCGTGTGGCCGATACCGCGAGCA‑GGTTTTCCCTCTGTTTGCGCGAATCGACAACAACTTGGCGTGATCGGTCAAGCCGGTTGACGCGTGGCGATCCGAGAATTGAGAAAAGACGGCAATCAAGAGGTACAGACATGAGG > AM260480/1166542‑1166790
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
BRESEQ :: bam2aln output
AGCCCTTGGAGGAACTGACGGCCCGGCATCCCGGACCCAACATGTGATGGCGAAGGCGGGACCCGCGACGGTGTGGGTCCCGCTCTTTCGCCTTGCTGCCGTGGACCCCCCAGACCTCAATTGCCGTGTGGCCGATACCGCGAGCA‑GGTTTTCCCTCTGTTTGCGCGAATCGACAACAACTTGGCGTGATCGGTCAAGCCGGTTGACGCGTGGCGATCCGAGAATTGAGAAAAGACGGCAATCAAGAGGTACAGACATGAGGAAGCGAACCAATAACCTATGGTGGCGCGGATAC > AM260480/1166529‑1166823
|
AGCCCTTGGAGGAACTGACGGCCCGGCATCCCGGACCCAACATGTGATGGCGAAGGCGGGACCCGCGACGGTGTGGGTCCCGCTCTTTCGCCTTGCTGCCGTGGACCCCCCAGACCTCAATTGCCGTGTGGCCGATACCGCGAGCA‑GGGT < GWNJ‑0478:712:GW2002102894th:2:1115:8899:29971/150‑1 (MQ=60)
TGGAGGAACTGACGGCCCGGCATCCCGGACCCAACATGTGATGGCGAAGGCGGGACCCGCGACGGTGTGGGTCCCGCTCTTTCGCCTTGCTGCCGTGGACCCCCCAGACCTCAATTGCCGTGTGGCCGATACCGCGAGCAGGGTTTTCCC > GWNJ‑0478:712:GW2002102894th:2:1115:17627:21152/1‑150 (MQ=60)
GAGGAACTGACGGCCCGGCATCCCGGACCCAACATGTGATGGCGAAGGCGGGACCCGCGACGGTGTGGGTCCCGCTCTTTCGCCTTGCTGCCGTGGACCCCCCAGACCTCAATTGCCGTGTGGCCGATACCGCGAGCAGGGTTTTCCCTC < GWNJ‑0478:712:GW2002102894th:2:1115:15258:95769/150‑1 (MQ=60)
GAGGAACTGACGGCCCGGCATCCCGGACCCAACATGTGATGGCGAAGGCGGGACCCGCGACGGTGTGGGTCCCGCTCTTTCGCCTTGCTGCCGTGGACCCCCCAGACCTCAATTGCCGTGTGGCCGATACCGCGAGCAGGGTTTTCCCTC < GWNJ‑0478:712:GW2002102894th:2:1208:7000:77212/150‑1 (MQ=60)
AACTGACGGCCCGGCATCCCGGACCCAACATGTGATGGCGAAGGCGGGACCCGCGACGGTGTGGGTCCCGCTCTTTCGCCTTGCTGCCGTGGACCCCCCAGACCTCAATTGCCGTGTGGCCGATACCGCGAGCAGGGTTTTCCCTCTGTT < GWNJ‑0478:712:GW2002102894th:2:1114:12807:75673/150‑1 (MQ=60)
gcatacgagatgtagctccgtctcgtgggctcggagatgtgtataagagacagGGTGTGGGTCCCGCTCTTTCGCCTTGCTGCCGTGGACCCCCCAGACCTCAATTGCCGTGTGGCCGATACCGCGAGCAGGGTTTTCCCTCTGTTTGCG < GWNJ‑0478:712:GW2002102894th:2:2215:19573:47176/97‑1 (MQ=60)
CCCAACATGTGATGGCGAAGGCGGGACCCGCGACGGTGTGGGTCCCGCTCTTTCGCCTTGCTGCCGTGGACCCCCCAGACCTCAATTGCCGTGTGGCCGATACCGCGAGCAGGGTTTTCCCTCTGTTTGCGCGAATCGACAACAACTTGG > GWNJ‑0478:712:GW2002102894th:2:2208:12363:40901/1‑150 (MQ=60)
CATGTGATGGCGAAGGCGGGACCCGCGACGGTGTGGGTCCCGCTCTTTCGCCTTGCTGCCGTGGACCCCCCAGACCTCAATTGCCGTGTGGCCGATACCGCGAGCAGGGTTTTCCCTCTGTTTGCGCGAATCGACAACAACctgtctctt > GWNJ‑0478:712:GW2002102894th:2:1114:10341:45413/1‑141 (MQ=60)
GTGTGGGTCCCGCTCTTTCGCCTTGCTGCCGTGGACCCCCCAGACCTCAATTGCCGTGTGGCCGATACCGCGAGCAGGGTCTTCCCTCTGTTTGCGCGAATCGACAACAACTTGGCGTGATAGGTCAAGCCGGTTGACGCGTGGCGATCC > GWNJ‑0478:712:GW2002102894th:2:1211:5645:53281/1‑150 (MQ=60)
GTCCCGCTCTTTCGCCTTGCTGCCGTGGACCCCCCAGACCTCAATTGCCGTGTGGCCGATACCGCGAGCAGGGTTTTCCCTCTGTTTGCGCGAATCGACAACctgtctcttatacacatctccgagcccacgagacggagctacatctcg > GWNJ‑0478:712:GW2002102894th:2:1209:8549:16615/1‑102 (MQ=60)
GTCCCGCTCTTTCGCCTTGCTGCCGTGGACCCCCCAGACCTCAATTGCCGTGTGGCCGATACCGCGAGCAGGGTTTTCCCTCTGTTTGCGCGAATCGACAACAACTTGGCGTGATCGGTCAAGCCGGTTGACGCGTGGCGATCCGAGAAT > GWNJ‑0478:712:GW2002102894th:2:2116:14211:91933/1‑150 (MQ=60)
cggggatgtgtataagagacagGTGGCCGATACCGCGAGCAGGGTTTTCCCTCTGTTTGCGCGAATCGACAACAACTTGGCGTGATCGGTCAAGCCGGTTGACGCGTGGCGATCCGAGAATTGAGAAAAGACGGCAATCAAGAGGTACAG < GWNJ‑0478:712:GW2002102894th:2:1113:13404:16893/128‑1 (MQ=60)
CCCCAGACCTCAATTGCCGTGTGGCCGATACCGCGAGCAGGGTTTTCCCTCTGTTTGCGCGAATCGACAACAACTTGGCGTGATCGGTCAAGCCGGTTGACGCGTGGCGATCCGAGAATTGAGAAAAGACGGCAATCAAGAGGTACAGAC > GWNJ‑0478:712:GW2002102894th:2:2206:3848:97082/1‑150 (MQ=60)
ccgtctcgtgggctcggagatgtgtataagagacaggGGTTTTCCCTCTGTTTGCGCGAATCGACAACAACTTGGCGTGATCGGTCAAGCCGGTTGACGCGTGGCGATCCGAGAATTGAGAAAAGACGGCAATCAAGAGGTACAGACA < GWNJ‑0478:712:GW2002102894th:2:2106:16311:76740/111‑1 (MQ=60)
CCTCAATTGCCGTGTGGCCGATACCGCGAGCAGGGTTTTCCCTCTGTTTGCGCGAATCGACAACAACTTGGCGTGATCGGTCAAGCCGGTTGACGCGTGGCGATCCGAGAATTGAAAAAAGACGGCAATCAAGAGGTACAGACATGAGG > GWNJ‑0478:712:GW2002102894th:2:1216:16000:34972/1‑149 (MQ=60)
CTCAATTGCCGTGTGGCCGATACCGCGAGCAGGGTTTTCCCTCTGTTTGCGCGAATCGACAACAACTTGGCGTGATCGGTCAAGCCGGTTGACGCGTGGCGATCCGAGAATTGAGAAAAGACGGCAATCAAGAGGTACAGACATGAGGA > GWNJ‑0478:712:GW2002102894th:2:2101:7696:29219/1‑149 (MQ=60)
GCCGTGTGGCCGATACCGCGAGCAGGGTTTTCCCTCTGTTTGCGCGAATCGACAACAACTTGGCGTGATCGGTCAAGCCGGTTGACGCGTGGCGATCCGAGAATTGAGAAAAGACGGCAATCAAGAGGTACAGACATGAGGAAGCGAACC < GWNJ‑0478:712:GW2002102894th:2:2105:17473:59014/150‑1 (MQ=60)
GTGGCCGATACCGCGAGCAGGGTTTTCCCTCTGTTTGCGCGAATCGACAACAACTTGGCGTGATCGGTCAAGCCGGTTGACGCGTGGCGATCCGAGAATTGAGAAAAGACGGCAATCAAGAGGTACAGACATGAGGAAGCGAACCAATA > GWNJ‑0478:712:GW2002102894th:2:1114:13186:92885/1‑149 (MQ=60)
GGCCGATACCGCGAGCAGGGTTTTCCCTCTGTTTGCGCGAATCGACAACAACTTGGCGTGATCGGTCAAGCCGGTTGACGCGTGGCGATCCGAGAATTGAGAAAAGACGGCAATCAAGAGGTACAGACActgtctcttatacacatctcc > GWNJ‑0478:712:GW2002102894th:2:1209:4126:51311/1‑129 (MQ=60)
GGCCGATACCGCGAGCAGGGTTTTCCCTCTGTTTGCGCGAATCGACAACAACTTGGCGTGATCGGTCAAGCCGGTTGACGCGTGGCGATCCGAGAATTGAGAAAAGACGGCAATCAAGAGGTACAGACATGAGGAAGCGAACCAATAACC > GWNJ‑0478:712:GW2002102894th:2:1215:11608:82967/1‑150 (MQ=60)
ataagagacaggGGTTTTCCCTCTGTTTGCGCGAATCGACAACAACTTGGCGTGATCGGTCAAGCCGGTTGACGCGTGGCGATCCGAGAATTGAGAAAAGACGGCAATCAAGAGGTACAGACATGAGGAAGCGAACCAATAACCTATGGT < GWNJ‑0478:712:GW2002102894th:2:1205:7219:68416/138‑1 (MQ=60)
cagGGTTTTCCCTCTGTTTGCGCGAATCGACAACAACTTGGCGTGATCGGTCAAGCCGGTTGACGCGTGGCGATCCGAGAATTGAGAAAAGACGGCAATCAAGAGGTACAGctgtctcttatacacatctccgagcccacgagacggagc > GWNJ‑0478:712:GW2002102894th:2:1101:4311:51341/4‑111 (MQ=60)
cagGGTTTTCCCTCTGTTTGCGCGAATCGACAACAACTTGGCGTGATCGGTCAAGCCGGTTGACGCGTGGCGATCCGAGAATTGAGAAAAGACGGCAATCAAGAGGTACAGctgtctcttatacacatctccgagcccacgagacggagc > GWNJ‑0478:712:GW2002102894th:2:2205:3027:99427/4‑111 (MQ=60)
G‑GGTTTTCCCTCTGTTTGCGCGAATCGACAACAACTTGGCGTGATCGGTCAAGCCGGTTGACGCGTGGCGATCCGAGAATTGAGAAAAGACGGCAATCAAGAGGTACAGACATGAGGAAGCGAACCAATAACCctgtctcttatacacat > GWNJ‑0478:712:GW2002102894th:2:1116:6100:2656/1‑133 (MQ=60)
G‑GGTTTTCCCTCTGTTTGCGCGAATCGACAACAACTTGGCGTGATCGGTCAAGCCGGTTGACGCGTGGCGATCCGAGAATTGAGAAAAGACGGCAATCAAGAGGTACAGACATGAGGAAGCGAACCAATAACCTATGGTctgtctcttat > GWNJ‑0478:712:GW2002102894th:2:2115:18883:92443/1‑139 (MQ=60)
|
AGCCCTTGGAGGAACTGACGGCCCGGCATCCCGGACCCAACATGTGATGGCGAAGGCGGGACCCGCGACGGTGTGGGTCCCGCTCTTTCGCCTTGCTGCCGTGGACCCCCCAGACCTCAATTGCCGTGTGGCCGATACCGCGAGCA‑GGTTTTCCCTCTGTTTGCGCGAATCGACAACAACTTGGCGTGATCGGTCAAGCCGGTTGACGCGTGGCGATCCGAGAATTGAGAAAAGACGGCAATCAAGAGGTACAGACATGAGGAAGCGAACCAATAACCTATGGTGGCGCGGATAC > AM260480/1166529‑1166823
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
