Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A0 F0 I1 R1
|
155 |
25.8 |
2285122 |
88.0% |
2010907 |
104.3 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
AM260480 |
1,192,562 |
+C |
coding (163/594 nt) |
cccA → |
cytochrome c, mono‑or diheme variant |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | AM260480 | 1,192,560 | 1 | . | C | 100.0%
| 95.0
/ NA
| 26 | V54A (GTC→GCC) | cccA | cytochrome c, mono‑or diheme variant |
| Reads supporting (aligned to +/- strand): ref base . (0/0); new base C (13/13); total (13/13) |
GCCTCCGGGCCTGGTCCGGCTGGCTGGTATGGCTCTTGCGGAAACAGGGCAAACAACCAAGACCCGTCCACTGTAGGAGACATCCATGAAAACGCAGCGCCGCCTGCTTGCCTTGCTCGCGCTTTGCGCGT‑CCTGACCGCCCTCCCGGCCGGCTCCGTGCTGGCCCATGGCGACGTCACGCCCCAGGCCGTCGATACCAAAGCGTTACCACAACTGGGCGACGAATGGCGCCCGGACAATCC > AM260480/1192430‑1192671
|
gcCTCCGGGCCTGGTCCGGCTGGCTGGTATGGCTCTTGCGGAAACAGGGCAAACAACCAAGACCCGTCCACTGTAGGAGACATCCATGAAAACGCAGCGCCGCCTGCTTGCCTTGCTCGCGCTTTGCGCGT‑ccc > 2:180626/1‑133 (MQ=255)
ggCCTGGTCCGGCTGGCTGGTATGGCTCTTGCGGAAACAGGGCAAACAACCAAGACCCGTCCACTGTAGGAGACATCCATGAAAACGCAGCGCCGCCTGCTTGCCTTGCTCGCGCTTTGCGCGTCCCTGACCGc < 2:529153/134‑1 (MQ=255)
ggctggctGGTATGGCTCTTGCGGAAACAGGGCAAACAACCAAGACCCGTCCACTGTAGGAGACATCCATGAAAACGCAGCGCCGCCTGCTTGCCTTGCTCGCGCTTTGCGCGTCCCTGACCGCCCTCccggcc < 1:180626/134‑1 (MQ=255)
ggctggctGGTATGGCTCTTGCGGAAACAGGGCAAACAACCAAGACCCGTCCACTGTAGGAGACATCCATGAAAACGCAGCGCCGCCTGCTTGCCTTGCTCGCGCTTTGCGCGTCCCTGACCGCCCTCccggcc < 1:207101/134‑1 (MQ=255)
aTGGCTCTTGCGGAAACAGGGCAAACAACCAAGACCCGTCCACTGTAGGAGACATCCATGAAAACGCAGCGCCGCCTGCTTGCCTTGCTCGCGCTTTGCGCGTCCCTGACCGCCCTCCCGGCCGGCTCCGTGCt > 1:360590/1‑134 (MQ=255)
tGCGGAAACAGGGCAAACAACCAAGACCCGTCCACTGTAGGAGACATCCATGAAAACGCAGCGCCGCCTGCTTGCCTTGCTCGCGCTTTGCGCGTCCCTGACCGCCCTCCCGGCCGGCTCCg < 2:540716/122‑1 (MQ=255)
tGCGGAAACAGGGCAAACAACCAAGACCCGTCCACTGTAGGAGACATCCATGAAAACGCAGCGCCGCCTGCTTGCCTTGCTCGCGCTTTGCGCGTCCCTGACCGCCCTCCCGGCCGGCTCCg > 1:540716/1‑122 (MQ=255)
gAAACAGGGCAAACAACCAAGACCCGTCCACTGTAGGAGACATCCATGAAAACGCAGCGCCGCCTGCTTGCCTTGCTCGCGCTTTGCGCGTCCCTGACCGCCCTCCCGGCCGGCTCCGTGCTGGCCCATGGCGa > 1:752690/1‑134 (MQ=255)
aaaCAGGGCAAACAACCAAGACCCGTCCACTGTAGGAGACATCCATGAAAACGCAGCGCCGCCTGCTTGCCTTGCTCGCGCTTTGCGCGTCCCTGACCGCCCTCCCGGCCGGCTCCGTGCTGGCCCATGGCGAc < 2:752690/134‑1 (MQ=255)
aacCAAGACCCGTCCACTGTAGGAGACATCCATGAAAACGCAGCGCCGCCTGCTTGCCTTGCTCGCGCTTTGCGCGTCCCTGACCGCCCTCCCGGCCGGCTCCGTGCTGGCCCATGGCGACGTCACGCCCCAgg < 2:9699/134‑1 (MQ=255)
cTGTAGGAGACATCCATGAAAACGCAGCGCCGCCTGCTTGCCTTGCTCGCGCTTTGCGCGTCCCTGACCGCCCTCCCGGCCGGCTCCGTGCTGGCCCATGGCGACGTCACGCCCCAGGCCGTCGATACCAAAGc > 1:414636/1‑134 (MQ=255)
cTGTAGGAGACATCCATGAAAACGCAGCGCCGCCTGCTTGCCTTGCTCGCGCTTTGCGCGTCCCTGACCGCCCTCCCGGCCGGCTCCGTGCTGGCCCATGGCGACGTCACGCCCCAGGCCGTCGATACCAAAGc > 1:174810/1‑134 (MQ=255)
tAGGAGACATCCATGAAAACGCAGCGCCGCCTGCTTGCCTTGCTCGCGCTTTGCGCGTCCCTGACCGCCCTCCCGGCCGGCTCCGTGCTGGCCCATGGCGACGTCACGCCCCAGGCCGTCGATACCAAAGCg < 2:174810/132‑1 (MQ=255)
cATCCATGAAAACGCAGCGCCGCCTGCTTGCCTTGCTCGCGCTTTGCGCGTCCCTGACCGCCCTCCCGGCCGGCTCCGTGCTGGCCCATGGCGAc > 1:672755/1‑95 (MQ=255)
cATCCATGAAAACGCAGCGCCGCCTGCTTGCCTTGCTCGCGCTTTGCGCGTCCCTGACCGCCCTCCCGGCCGGCTCCGTGCTGGCCCATGGCGAc < 2:672755/95‑1 (MQ=255)
aTGAAAACGCAGCGCCGCCTGCTTGCCTTGCTCGCGCTTTGCGCGTCCCTGACCGCCCTCCCGGCCGGCTCCGTGCTGGCCCATGGCGACGTCACGccc > 2:1127775/1‑99 (MQ=255)
aTGAAAACGCAGCGCCGCCTGCTTGCCTTGCTCGCGCTTTGCGCGTCCCTGACCGCCCTCCCGGCCGGCTCCGTGCTGGCCCATGGCGACGTCACGccc < 1:1127775/99‑1 (MQ=255)
gAAAACGCAGCGCCGCCTGCTTGCCTTGCTCGCGCTTTGCGCGTCCCTGACCGCCCTCCCGGCCGGCTCCGTGCTGGCCCATGGCGACGTCACGCCCCAGGCCGTCGATa < 1:468764/110‑1 (MQ=255)
gAAAACGCAGCGCCGCCTGCTTGCCTTGCTCGCGCTTTGCGCGTCCCTGACCGCCCTCCCGGCCGGCTCCGTGCTGGCCCATGGCGACGTCACGCCCCAGGCCGTCGATa > 2:468764/1‑110 (MQ=255)
gAAAACGCAGCGCCGCCTGCTTGCCTTGCTCGCGCTTTGCGCGTCCCTGACCGCCCTCCCGGCCGGCTCCGTGCTGGCCCATGGCGACGTCACGCCCCAGGCCGTCGATACCAAAGCGTTACCACAACTgg < 2:347867/131‑1 (MQ=255)
gAAAACGCAGCGCCGCCTGCTTGCCTTGCTCGCGCTTTGCGCGTCCCTGACCGCCCTCCCGGCCGGCTCCGTGCTGGCCCATGGCGACGTCACGCCCCAGGCCGTCGATACCAAAGCGTTACCACAACTgg > 1:347867/1‑131 (MQ=255)
gAAAACGCAGCGCCGCCTGCTTGCCTTGCTCGCGCTTTGCGCGTCCCTGACCGCCCTCCCGGCCGGCTCCGTGCTGGCCCATGGCGACGTCACGCCCCAGGCCGTCGATACCAAAGCGTTACCACAACTGGGc > 1:532156/1‑133 (MQ=255)
gAAAACGCAGCGCCGCCTGCTTGCCTTGCTCGCGCTTTGCGCGTCCCTGACCGCCCTCCCGGCCGGCTCCGTGCTGGCCCATGGCGACGTCACGCCCCAGGCCGTCGATACCAAAGCGTTACCACAACTGGGc > 2:538677/1‑133 (MQ=255)
gAAAACGCAGCGCCGCCTGCTTGCCTTACTCGCGCTTTGCGCGTCCCTGACCGCCCTCCCGGCCGGCTCCGTGCTGGCCCATGGCGACGTCACGCCCCAGGCCGTCGATACCAAAGCGTTACCACAACTGGGc > 1:634320/1‑133 (MQ=255)
aaaCGCAGCGCCGCCTGCTTGCCTTGCTCGCGCTTTGCGCGTCCCTGACCGCCCTCCCGGCCGGCTCCGTGCTGGCCCATGGCGACGTCACGCCCCAGGCCGTCGATACCAAAGCGTTACCACAACTGGGcgac < 2:360590/134‑1 (MQ=255)
cAGCGCCGCCTGCTTGCCTTGCTCGCGCTTTGCGCGTCCCTGACCGCCCTCCCGGCCGGCTCCGTGCTGGCCCATGGCGACGTCACGCCCCAGGCCGTCGATACCAAAGCGTTACCACAACTGGGCGACGAATg > 1:280558/1‑134 (MQ=255)
ccttgcTCGCGCTTTGCGCGTCCCTGACCGCCCTCCCGGCCGGCTCCGTGCTGGCCCATGGCGACGTCACGCCCCAGGCCGTCGATACCAAAGCGTTACCACAACTGGGCGACGAATGGCGCCCGGACAATcc < 2:532156/133‑1 (MQ=255)
|
GCCTCCGGGCCTGGTCCGGCTGGCTGGTATGGCTCTTGCGGAAACAGGGCAAACAACCAAGACCCGTCCACTGTAGGAGACATCCATGAAAACGCAGCGCCGCCTGCTTGCCTTGCTCGCGCTTTGCGCGT‑CCTGACCGCCCTCCCGGCCGGCTCCGTGCTGGCCCATGGCGACGTCACGCCCCAGGCCGTCGATACCAAAGCGTTACCACAACTGGGCGACGAATGGCGCCCGGACAATCC > AM260480/1192430‑1192671
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
BRESEQ :: bam2aln output
TGCTTTCAGGCGCCTCCGGGCCTGGTCCGGCTGGCTGGTATGGCTCTTGCGGAAACAGGGCAAACAACCAAGACCCGTCCACTGTAGGAGACATCCATGAAAACGCAGCGCCGCCTGCTTGCCTTGCTCGCGCTTTGCGCGT‑CCTGACCGCCCTCCCGGCCGGCTCCGTGCTGGCCCATGGCGACGTCACGCCCCAGGCCGTCGATACCAAAGCGTTACCACAACTGGGCGACGAATGGCGCCCGGACAATCCCTACCGTAGCGGTGATGCGCACAAGGAGGCTCTGCGG > AM260480/1192419‑1192708
|
cgagattagcgagtgtctcgtgggctcggagatgtgtataagagacagGCGGAAACAGGGCAAACAACCAAGACCCGTCCACTGTAGGAGACATCCATGAAAACGCAGCGCCGCCTGCTTGCCTTGCTCGCGCTTTGCGCGTCCCTGACC < GWNJ‑0478:712:GW2002102894th:2:2215:19592:69424/102‑1 (MQ=60)
GTCCGGCTGGCTGGTATGGCTCTTGCGGAAACAGGGCAAACAACCAAGACCCGTCCACTGTAGGAGACATCCATGAAAACGCAGCGCCGCCTGCTTGCCTTGCTCGCGCTTTGCGCGTCCCTGACCGCCCTCCCGGCCGGCTCCGTGCTG > GWNJ‑0478:712:GW2002102894th:2:1204:13591:87434/1‑150 (MQ=60)
CGGCTGGCTGGTATGGCTCTTGCGGAAACAGGGCAAACAACCAAGACCCGTCCACTGTAGGAGACATCCATGAAAACGCAGCGCCGCCTGCTTGCCTTGCTCGCGCTTTGCGCGTCCCTGACCGCCCTCCCGGCCGGCTCCGTGCTGGCC < GWNJ‑0478:712:GW2002102894th:2:1110:14477:69759/150‑1 (MQ=60)
CGGCTGGCTGGTATGGCTCTTGCGGAAACAGGGCAAACAACCAAGACCCGTCCACTGTAGGAGACATCCATGAAAACGCAGCGCCGCCTGCTTGCCTTGCTCGCGCTTTGCGCGTCCCTGACCGCCCTCCCGGCCGGCTCCGTGCTGGCC < GWNJ‑0478:712:GW2002102894th:2:1112:10139:8568/150‑1 (MQ=60)
GGCTGGTATGGCTCTTGCGGAAACAGGGCAAACAACCAAGACCCGTCCACTGTAGGAGACATCCATGAAAACGCAGCGCCGCCTGCTTGCCTTGCTCGCGCTTTGCGCGTCCCTGACCGCCCTCCCGGCCGGCTCCGTGCTGGCCCATGG > GWNJ‑0478:712:GW2002102894th:2:1214:17317:30023/1‑150 (MQ=60)
GGTATGGCTCTTGCGGAAACAGGGCAAACAACCAAGACCCGTCCACTGTAGGAGACATCCATGAAAACGCAGCGCCGCCTGCTTGCCTTGCTCGCGCTTTGCGCGTCCCTGACCGCCCTCCCGGCCGGCTCCGTGCTGGCCCATGGCGAC > GWNJ‑0478:712:GW2002102894th:2:2110:10226:73352/1‑150 (MQ=60)
ggagatgtgtataagagacagCTGTAGGAGACATCCATGAAAACGCAGCGCCGCCTGCTTGCCTTGCTCGCGCTTTGCGCGTCCCTGACCGCCCTCCCGGCCGGCTCCGTGCTGGCCCATGGCGACGTCACGCCCCAGGCCGTCGATACC < GWNJ‑0478:712:GW2002102894th:2:2216:4157:27901/129‑1 (MQ=60)
ACTAAGACCCGTCCACTGTAGGAGACATCCATGAAAACGCAGCGCCGCCTGCTTGCCTTGCTCGCGCTTTGCGCGTCCCTGACCGCCCTCCCGGCCGGCTCCGTGCTGGCCCATGGCGACGTCACGCCCCAGGCCGTCGATACCAAAGCG > GWNJ‑0478:712:GW2002102894th:2:1208:3381:93551/1‑150 (MQ=60)
ACCAAGACCCGTCCACTGTAGGAGACATCCATGAAAACGCAGCGCCGCCTGCTTGCCTTGCTCGCGCTTTGCGCGTCCCTGACCGCCCTCCCGGCCGGCTCCGTGCTGGCCCATGGCGACGTCACGCCCCAGGCCGTCGATACCAAAGCG > GWNJ‑0478:712:GW2002102894th:2:1110:16902:38905/1‑150 (MQ=60)
aagagacagGTAGGAGACATCCATGAAAACGCAGCGCCGCCTGCTTGCCTTGCTCGCGCTTTGCGCGTCCCTGACCGCCCTCCCGGCCGGCTCCGTGCTGGCCCATGGCGACGTCACGCCCCAGGCCGTCGATACCAAAGCGTTACCAC < GWNJ‑0478:712:GW2002102894th:2:1210:11439:79068/140‑1 (MQ=60)
ggcatacgagattagcgagtgtctcgtgggctcggagatgtgtataagagacaGCGCTTTGCGCGTCCCTGACCGCCCTCCCGGCCGGCTCCGTGCTGGCCCATGGCGACGTCACGCCCCAGGCCGTCGATACCAAAGCGTTACCACAAC < GWNJ‑0478:712:GW2002102894th:2:2214:11070:52424/97‑1 (MQ=60)
GTCCACTGTAGGAGACATCCATGAAAACGCAGCGCCGCCTGCTTGCCTTGCTCGCGCTTTGCGCGTCCCTGACCGCCCTCCCGGCCGGCTCCGTGCTGGCCCATGGCGACGTCACGCCCCAGGCCctgtctcttatacacatctccgagc > GWNJ‑0478:712:GW2002102894th:2:2106:13081:22712/1‑125 (MQ=60)
GTAGGAGACATCCATGAAAACGCAGCGCCGCCTGCTTGCCTTGCTCGCGCTTTGCGCGTCCCTGACCGCCCTCCCGGCCGGCTCCGTGCTGGCCCATGGCGACGTCACGCCCCAGGCCGTCGATACCAAAGCGTTACCACAACTGGGCG > GWNJ‑0478:712:GW2002102894th:2:1204:18958:10056/1‑149 (MQ=60)
GTAGGAGACATCCATGAAAACGCAGCGCCGCCTGCTTGCCTTGCTCGCGCTTTGCGCGTCCCTGACCGCCCTCCCGGCCGGCTCCGTGCTGGCCCATGGCGACGTCACGCCCCAGGCCGTCGATACCAAAGCGTTACCACAACTGGGCG > GWNJ‑0478:712:GW2002102894th:2:1215:5639:89622/1‑149 (MQ=60)
GTAGGAGACATCCATGAAAACGCAGCGCCGCCTGCTTGCCTTACTCGCGCTTTGCGCGTCCCTGACCGCCCTCCCGGCCGGCTCCGTGCTGGCCCATGGCGACGTCACGCCCCAGGCCGTCGATACCAAAGCGTTACCACAACTGGGCG > GWNJ‑0478:712:GW2002102894th:2:2105:10328:89547/1‑149 (MQ=60)
ACATCCATGAAAACGCAGCGCCGCCTGCTTGCCTTGCTCGCGCTTTGCGCGTCCCTGACCGCCCTCCCGGCCGGCTCCGTGCTGGCCCATGGCGACGTCACGCCCCAGGCCGTCGATACCAAAGCGTTACCACAACTGGGCGACGAATGG > GWNJ‑0478:712:GW2002102894th:2:1116:9443:87908/1‑150 (MQ=60)
TGCTCGCGCTTTGCGCGTCCCTGACCGCCCTCCCGGCCGGCTCCGTGCTGGCCCATGGCGACGTCACGCCCCAGGCCGTCGATACCAAAGCGTTACCACAACctgtctcttatacacatctccgagcccacgagacactcgctaatctcg > GWNJ‑0478:712:GW2002102894th:2:1212:9184:97691/1‑102 (MQ=60)
CGCTTTGCGCGTCCCTGACCGCCCTCCCGGCCGGCTCCGTGCTGGCCCATGGCGACGTCACGCCCCAGGCCtgtctcttatacacatctcccgagcccacgagacactcgctaatctcgtatgccgtcttctgcttgaaaaaaaaaaac > GWNJ‑0478:712:GW2002102894th:2:1111:10922:100393/1‑71 (MQ=60)
gtcCCTGACCGCCCTCCCGGCCGGCTCCGTGCTGGCCCATGGCGACGTCACGCCCCAGGCCGTCGATACCAAAGCGTTACCACAACTGGGCGACGAATGGCGCCCGGACAATCCCTACCGTAGCGGTGATGCGCACAAGGAGGCTCTGCG < GWNJ‑0478:712:GW2002102894th:2:1214:20855:20729/147‑1 (MQ=60)
tcCCTGACCGCCCTCCCGGCCGGCTCCGTGCTGGCCCATGGCGACGTCACGCCCCctgtctcttatacacatctccgagcccacgagacactcgctaatctcgtatgccgtcttctgcttgaaaaaaaaaaaaacaagcctcgtagaaa > GWNJ‑0478:712:GW2002102894th:2:1214:13732:96604/3‑55 (MQ=60)
tcCCTGACCGCCCTCCCGGCCGGCTCCGTGCTGGCCCATGGCGACGTCACGCCCCAGGCCGTCGATACCAAAGCGTTACCACAACctgtctcttatacacatctccgagcccacgagacactcgctaatctcgtatgccgtcttctgctt > GWNJ‑0478:712:GW2002102894th:2:2206:8281:48672/3‑85 (MQ=60)
tcCCTGACCGCCCTCCCGGCCGGCTCCGTGCTGGCCCATGGCGACGTCACGCCCCAGGCCGTCGATACCAAAGCGTTACCACAACTGGGCGACGAATGGCGCCCGGACAATCCCTgtctcttatacacatctccgagcccacgagacact > GWNJ‑0478:712:GW2002102894th:2:2101:15744:44995/3‑115 (MQ=60)
|
TGCTTTCAGGCGCCTCCGGGCCTGGTCCGGCTGGCTGGTATGGCTCTTGCGGAAACAGGGCAAACAACCAAGACCCGTCCACTGTAGGAGACATCCATGAAAACGCAGCGCCGCCTGCTTGCCTTGCTCGCGCTTTGCGCGT‑CCTGACCGCCCTCCCGGCCGGCTCCGTGCTGGCCCATGGCGACGTCACGCCCCAGGCCGTCGATACCAAAGCGTTACCACAACTGGGCGACGAATGGCGCCCGGACAATCCCTACCGTAGCGGTGATGCGCACAAGGAGGCTCTGCGG > AM260480/1192419‑1192708
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
