Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A0 F0 I1 R1
|
155 |
25.8 |
2285122 |
88.0% |
2010907 |
104.3 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
AM260480 |
2,063,918 |
G→A |
P215L (CCG→CTG) |
h16_B1826 ← |
Acyl‑CoA dehydrogenase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | AM260480 | 2,063,918 | 0 | G | A | 100.0%
| 68.0
/ NA
| 19 | P215L (CCG→CTG) | h16_B1826 | Acyl‑CoA dehydrogenase |
| Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (10/9); total (10/9) |
ATGCAATGCGACAGCCGCGCCGGCGACAAGCGAACCTGCGCATAACGGAAGCCCTCGTCCACCTCGCCCAGCACCTGGTCCTGCGGCACGCGCAGGCCGTCGATCAGGACCTGCGCATGTCCGCCCGGCATGGAACTGTCGATGGTGTCAAGCAGGCGTTCGATGCGGATCGCCGGATGGGGCAGGTCGACCAGGAACATGGTGGCCTGCGCGCGCTCACCATCGCCGGTGCGCGCCATCAGGATGCC > AM260480/2063792‑2064039
|
aTGCAATGCGACAGCCGCGCCGGCGACAAGCGAACCTGCGCATAACGGAAGCCCTCGTCCACCTCGCCCAGCACCTGGTCCTGCGGCACGCGCAGGCCGTCGATCAGGACCTGCGCATGTCCGCCCAGCATgg > 2:497804/1‑133 (MQ=255)
cGACAGCCGCGCCGGCGACAAGCGAACCTGCGCATAACGGAAGCCCTCGTCCACCTCGCCCAGCACCTGGTCCTGCGGCACGCGCAGGCCGTCGATCAGGACCTGCGCATGTCCGCCCAGCATGGAACTGTCg < 2:723329/133‑1 (MQ=255)
gcCGGCGACAAGCGAACCTGCGCATAACGGAAGCCCTCGTCCACCTCGCCCAGCACCTGGTCCTGCGGCACGCGCAGGCCGTCGATCAGGACCTGCGCATGTCCGCCCAGCATGGAACTGTCGATGGTGTCAAg > 2:472297/1‑134 (MQ=255)
tGCGCATAACGGAAGCCCTCGTCCACCTCGCCCAGCACCTGGTCCTGCGGCACGCGCAGGCCGTCGATCAGGACCTGCGCATGTCCGCCCAGCATGGAACTGTCGATGGTGTCAAGCAGGCGTTCGATGCGGAt > 2:1076403/1‑134 (MQ=255)
cGGAAGCCCTCGTCCACCTCGCCCAGCACCTGGTCCTGCGGCACGCGCAGGCCGTCGATCAGGACCTGCGCATGTCCGCCCAGCATGGAACTGTCGATGGTGTCAAGCAGGCGTTCGATGCGGATCGCCGGATg < 2:57398/134‑1 (MQ=255)
cACCTCGCCCAGCACCTGGTCCTGCGGCACGCGCAGGCCGTCGATCAGGACCTGCGCATGTCCGCCCAGCATGGAACTGTCGATGGTGTCAAGCAGGCGTTCGATGCGGATCGCCGGATGGGGCAGGTCGAcc < 1:497804/133‑1 (MQ=255)
cccAGCACCTGGTCCTGCGGCACGCGCAGGCCGTCGATCAGGACCTGCGCATGTCCGCCCAGCATGGAACTGTCGATGGTGTCAAGCAGGCGTTCGATGCgg > 1:439275/1‑102 (MQ=255)
cccAGCACCTGGTCCTGCGGCACGCGCAGGCCGTCGATCAGGACCTGCGCATGTCCGCCCAGCATGGAACTGTCGATGGTGTCAAGCAGGCGTTCGATGCgg < 2:439275/102‑1 (MQ=255)
tGCGGCACGCGCAGGCCGTCGATCAGGACCTGCGCATGTCCGCCCAGCATGGAACTGTCGATGGTGTCAAGCAGGCGTTCGATGCgg > 2:621703/1‑87 (MQ=255)
tGCGGCACGCGCAGGCCGTCGATCAGGACCTGCGCATGTCCGCCCAGCATGGAACTGTCGATGGTGTCAAGCAGGCGTTCGATGCgg < 1:621703/87‑1 (MQ=255)
gCACGCGCAGGCCGTCGATCAGGACCTGCGCATGTCCGCCCAGCGTGGAACTGTCGATGGTGTCAAGCAGGCGTTCGATGCGGATCGCCGGATGGGGCAGGTc < 2:1028258/103‑1 (MQ=255)
gCACGCGCAGGCCGTCGATCAGGACCTGCGCATGTCCGCCCAGCATGGAACTGGCGATGGTGTCAAGCAGGCGTTCGATGCGGATCGCCGGATGGGGCAGGTc > 1:1028258/1‑103 (MQ=255)
cAGGCCGTCGATCAGGACCTGCGCATGTCCGCCCAGCATGGAACTGTCGATGGTGTCAAGCAGGCGTTCGATGCGGATCGCCGGATGGGGCAGGTCGACCAGGAACATGGTGGCCTGCGCGCGCTCACCATCGc > 2:360419/1‑134 (MQ=255)
ccTGCGCATGTCCGCCCAGCATGGAACTGTCGATGGTGTCAAGCAGGCGTTc < 1:462445/52‑1 (MQ=255)
ccTGCGCATGTCCGCCCAGCATGGAACTGTCGATGGTGTCAAGCAGGCGTTc > 2:462445/1‑52 (MQ=255)
aTGTCCGCCCAGCATGGAACTGTCGATGGTGTCAAGCAgg > 2:25397/1‑40 (MQ=255)
aTGTCCGCCCAGCATGGAACTGTCGATGGTGTCAAGCAgg < 1:25397/40‑1 (MQ=255)
tGTCCGCCCAGCATGGAACTGTCGATGGTGTCAAGCAGGCGTTCGATGCGGATCGCCGGATGGGGCAGGTCGACCAGGAACATGGTGGCCTGCGCGCGCTCACCATCGCCGGTGCGCGCCATCAGGATGcc > 2:352722/1‑131 (MQ=255)
tGTCCGCCCAGCATGGAACTGTCGATGGTGTCAAGCAGGCGTTCGATGCGGATCGCCGGATGGGGCAGGTCGACCAGGAACATGGTGGCCTGCGCGCGCTCACCATCGCCGGTGCGCGCCATCAGGATGcc < 1:352722/131‑1 (MQ=255)
|
ATGCAATGCGACAGCCGCGCCGGCGACAAGCGAACCTGCGCATAACGGAAGCCCTCGTCCACCTCGCCCAGCACCTGGTCCTGCGGCACGCGCAGGCCGTCGATCAGGACCTGCGCATGTCCGCCCGGCATGGAACTGTCGATGGTGTCAAGCAGGCGTTCGATGCGGATCGCCGGATGGGGCAGGTCGACCAGGAACATGGTGGCCTGCGCGCGCTCACCATCGCCGGTGCGCGCCATCAGGATGCC > AM260480/2063792‑2064039
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
BRESEQ :: bam2aln output
ACCCCCGAACCAGCGCATGCAATGCGACAGCCGCGCCGGCGACAAGCGAACCTGCGCATAACGGAAGCCCTCGTCCACCTCGCCCAGCACCTGGTCCTGCGGCACGCGCAGGCCGTCGATCAGGACCTGCGCATGTCCGCCCGGCATGGAACTGTCGATGGTGTCAAGCAGGCGTTCGATGCGGATCGCCGGATGGGGCAGGTCGACCAGGAACATGGTGGCCTGCGCGCGCTCACCATCGCCGGTGCGCGCCATCAGGATGCCGGCGCTGGCCCCTTCGGCT > AM260480/2063776‑2064058
|
agatgtgtataagagacaGCAATGCGACAGCCGCGCCGGCGACAAGCGAACCTGCGCATAACGGAAGCCCTCGTCCACCTCGCCCAGCACCTGGTCCTGCGGCACGCGCAGGCCGTCGATCAGGACCTGCGCATGTCCGCCCAGCATGGA < GWNJ‑0478:712:GW2002102894th:2:1207:19120:30654/132‑1 (MQ=60)
agatgtgtataagagacagATGCGACAGCCGCGCCGGCGACAAGCGAACCTGCGCATAACGGAAGCCCTCGTCCACCTCGCCCAGCACCTGGTCCTGCGGCACGCGCAGGCCGTCGATCAGGACCTGCGCATGTCCGCCCAGCATGGAAC < GWNJ‑0478:712:GW2002102894th:2:2108:7225:76055/131‑1 (MQ=60)
tttttttttttttcaagcagaagacggcatacgagattagcgagtgtctcgtgggctcggagatgtgtataagagacaGCCCAGCACCTGGTCCTGCGGCACGCGCAGGCCGTCGATCAGGACCTGCGCATGTCCGCCCAGCATGGAAC < GWNJ‑0478:712:GW2002102894th:2:1112:10970:64189/71‑1 (MQ=60)
agcgttttttttttcaagcagaatacggcatacgagattagcgagtgtctcgtgggctcggagatgtgtataagagacagGATCAGGACCTTCGCATGTCCGCCCAGCATGGAACTGTCGATGGTGTCAAGCAGGCGTTCGATGCGGATC < GWNJ‑0478:712:GW2002102894th:2:1103:11298:64171/70‑1 (MQ=60)
tcaagcagaagacggcatacgagattagcgagtgtctcgtgggctcggagatgtgtataagagacagGGCCGTCGATCAGGACCTGCGCATGTCCGCCCAGCATGGAACTGTCGATGGTGTCAAGCAGGCGTTCGATGCGGATCGCCGG < GWNJ‑0478:712:GW2002102894th:2:1210:15123:46544/82‑1 (MQ=60)
cgtgggctcggagatgtgtataagagacaGCCCAGCACCTGGTCCTGCGGCACGCGCAGGCCGTCGATCAGGACCTGCGCATGTCCGCCCAGCATGGAACTGTCGATGGTGTCAAGCAGGCGTTCGATGCGGATCGCCGGATGGGGC < GWNJ‑0478:712:GW2002102894th:2:2105:9404:13194/118‑1 (MQ=60)
GCGCATAACGGAAGCCCTCGTCCACCTCGCCCAGCACCTGGTCCTGCGGCACGCGCAGGCCGTCGATCAGGACCTGCGCATGTCCGCCCAGCATGGAACctgtctcttatacacatctccgagcccacgagacactcgctaatctcgtat > GWNJ‑0478:712:GW2002102894th:2:2114:2394:83163/1‑99 (MQ=60)
cctgcgcatgtccgcccagcatggaactgtcgatggtgtcaaGCGCAGGCCGTCGATCAGGACCCCTTGGCCGGCCAATTGGTTCCCCGGCCCCCCAAGGCCAATTGGGGAAAACCTTGGTTCCGGAATTGGGGTTGGTTCCAAAAGGCCAAGGGGCCGGTTCGATGCGGATCGCCGGATGGGGC < GWNJ‑0478:712:GW2002102894th:2:2115:19754:94077/7‑4294967210 (MQ=60)
CCCTCGTCCACCTCGCCCAGCACCTGGTCCTGCGGCACGCGCAGGCCGTCGATCAGGACCTGCGCATGTCCGCCCAGCATGGAACTGTCGATGGTGTCAAGCAGGCGTTCGATGCGGATCGCCGGATGGGGCctgtctcttatacacatc > GWNJ‑0478:712:GW2002102894th:2:1211:18895:26555/1‑132 (MQ=60)
CCACCTCGCCCAGCACCTGGTCCTGCGGCACGCGCAGGCCGTCGATCAGGACCTGCGCATGTCCGCCCAGCATGGAACTGTCGATGGTGTCAAGCAGGCGTTCGATGCGGATCGCCGGATGGGGCAGGTCGACCAGGAACATGGTGGCC < GWNJ‑0478:712:GW2002102894th:2:1212:8214:23764/149‑1 (MQ=60)
GCACCTGGTCCTGCGGCACGCGCAGGCCGTCGATCAGGACCTGCGCATGTCCGCCCAGCATGGAACTGGCGATGGTGTCAAGCAGGCGTTCGATGCGGATCGCCGGATGGGGCAGGTCGACCAGGAACATGGTctgtctcttatacacat > GWNJ‑0478:712:GW2002102894th:2:2210:3567:29085/1‑133 (MQ=60)
agatgtgtataagagacaGGCATGGAACTGTCGATGGTGTCAAGCAGGCGTTCGATGCGGATCGCCGGATGGGGCAGGTCGACCAGGAACATGGTGGCCTGCGCGCGCTCACCATCGCCGGTGCGCGCCATCAGGATGCCGGCGCTGGCC < GWNJ‑0478:712:GW2002102894th:2:2103:18056:92891/132‑1 (MQ=60)
CGCATGTCCGCCCAGCATGGAACTGTCGATGGTGTCAAGCAGGCGTTCGATGCGGATCGCCGGATGGGGCAGGTCGACCAGGAACATGGTGGCCTGCGCGCGCTCACCATCGCCGGTGCGCGCCATCAGGATGCCGGCGCTGGCCCCTTC < GWNJ‑0478:712:GW2002102894th:2:1204:18912:39313/150‑1 (MQ=60)
CATGTCCGCCCAGCATGGAACTGTCGATGGTGTCAAGCAGGCGTTCGATGCGGATCGCCGGATGGGGCAGGTCGACCAGGAACATGGTGGCCTGCGCGCGCTCACCATCGCCGGTGCGCGCCATCAGGATGCCGGCGCTGGCCCCTTCGG > GWNJ‑0478:712:GW2002102894th:2:1114:11207:7070/1‑150 (MQ=60)
ATGTCCGCCCAGCATGGAACTGTCGATGGTGTCAAGCAGGCGTTCGATGCGGATCGCCGGATGGGGCAGGTCGACCAGGAACATGGTGGCCTGCGCGCGCTCACCATCGCCGGTGCGCGCCATCAGGATGCCGGCGCTGGCCCCTTCGGC < GWNJ‑0478:712:GW2002102894th:2:1211:13245:2379/150‑1 (MQ=60)
TGTCCGCCCAGCATGGAACTGTCGATGGTGTCAAGCAGGCGTTCGATGCGGATCGCCGGATGGGGCctgtctcttatacacatctccgagcccacgagacactcgctaatctcgtatgccgtcttctgcttgaaaaaaaaaataa > GWNJ‑0478:712:GW2002102894th:2:1213:10365:32689/1‑66 (MQ=60)
TGTCCGCCCAGCATGGAACTGTCGATGGTGTCAAGCAGGCGTTCGATGCGGATCGCCGGATGGGGCAGGTCGACCAGGAACATGGTGGCCTGCGCGCGCTCACCATCGCCGGTGCGCGCCATCAGGATGCCGGCGCTGGCCCCTTCGGCT > GWNJ‑0478:712:GW2002102894th:2:1215:16474:92730/1‑150 (MQ=60)
TGTCCGCCCAGCATGGAACTGTCGATGGTGTCAAGCAGGCGTTCGATGCGGATCGCCGGATGGGGCAGGTCGACCAGGAACATGGTGGCCTGCGCGCGCTCACCATCGCCGGTGCGCGCCATCAGGATGCCGGCGCTGGCCCCTTCGGCT > GWNJ‑0478:712:GW2002102894th:2:2210:20558:49225/1‑150 (MQ=60)
|
ACCCCCGAACCAGCGCATGCAATGCGACAGCCGCGCCGGCGACAAGCGAACCTGCGCATAACGGAAGCCCTCGTCCACCTCGCCCAGCACCTGGTCCTGCGGCACGCGCAGGCCGTCGATCAGGACCTGCGCATGTCCGCCCGGCATGGAACTGTCGATGGTGTCAAGCAGGCGTTCGATGCGGATCGCCGGATGGGGCAGGTCGACCAGGAACATGGTGGCCTGCGCGCGCTCACCATCGCCGGTGCGCGCCATCAGGATGCCGGCGCTGGCCCCTTCGGCT > AM260480/2063776‑2064058
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
