Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A0 F0 I1 R1
|
155 |
25.8 |
2285122 |
88.0% |
2010907 |
104.3 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
AM260480 |
2,843,580 |
+G |
intergenic (‑1/+68) |
pip ← / ← h16_B2516 |
prolyl aminopeptidase/transcriptional regulator, LysR‑family |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | AM260480 | 2,843,579 | 1 | . | G | 97.9%
| 178.7
/ ‑3.8
| 48 | coding (1/957 nt) | pip | prolyl aminopeptidase |
| Reads supporting (aligned to +/- strand): ref base . (0/1); new base G (25/22); total (25/23) |
| Fisher's exact test for biased strand distribution p-value = 4.79e-01 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
CGGCTTGGCGCCGGGCGTGCCCACACGCTCGTAGTAGATGACATGCCCGTCGCCGACATCCAGCGTGCCGGTTTCATAGGGCTCGATTTCGGGATAGAGGGTGCGCAAGGCCGGCA‑T‑GATATGGGTCTACGGAGATCGGGAGCCACAGTGTACGCCGGAGGGAACGTCAAACAGTAGCCCGCCCCTTAACCGGCGAACACCGCCTTCACCCAGTCCCTCAGCAACCGCGTGGCGGGACGC > AM260480/2843463‑2843702
|
cgGCTTGGCGCCGGGCGTGCCCACACGCTCGTAGTAGATGACATGCCCGTCGCCGACATCCAGCGTGCCGGTTTCATAGGGCTCGATTTCGGGATAGAGGGTGCGCAAGGCCGGCA‑TGGATATGGGTCTACg > 1:862465/1‑132 (MQ=255)
cgcCGGGCGTGCCCACACGCTCGTAGTAGATGACATGCCCGTCGCCGACATCCAGCGTGCCGGTTTCATAGGGCTCGATTTCGGGATAGAGGGTGCGCAAGGCCGGCA‑TGGATATGGGTCTACGGAGATCGGGa < 2:862465/134‑1 (MQ=255)
cgcCGGGCGTGCCCACACGCTCGTAGTAGATGACATGCCCGTCGCCGACATCCAGCGTGCCGGTTTCATAGGGCTCGATTTCGGGATAGAGGGTGCGCAAGGCCGGCA‑TGGATATGGGTCTACGGAGATCGGGa < 2:445169/134‑1 (MQ=255)
cGGGCGTGCCCACACGCTCGTAGTAGATGACATGCCCGTCGCCGACATCCAGCGTGCCGGTTTCATAGGGCTCGATTTCGGGATAGAGGGTGCGCAAGGCCGGCA‑TGGATATGGGTCTACGGAGATCGGGAGc > 1:1123898/1‑133 (MQ=255)
agtagATGACATGCCCGTCGCCGACATCCAGCGTGCCGGTTTCATAGGGCTCGATTTCGGGATAGAGGGTGCGCAAGGCCGGCA‑TGGATATggg > 2:608112/1‑94 (MQ=255)
agtagATGACATGCCCGTCGCCGACATCCAGCGTGCCGGTTTCATAGGGCTCGATTTCGGGATAGAGGGTGCGCAAGGCCGGCA‑TGGATATggg < 1:608112/94‑1 (MQ=255)
agtagATGACATGCCCGTCGCCGACATCCAGCGTGCCGGTTTCATAGGGCTCGATTTCGGGATAGAGGGTGCGCAAGGCCGGCA‑TGGATATggg < 1:485282/94‑1 (MQ=255)
agtagATGACATGCCCGTCGCCGACATCCAGCGTGCCGGTTTCATAGGGCTCGATTTCGGGATAGAGGGTGCGCAAGGCCGGCA‑TGGATATggg > 2:485282/1‑94 (MQ=255)
aCATGCCCGTCGCCGACATCCAGCGTGCCGGTTTCATAGGGCTCGATTTCGGGATAGAGGGTGCGCAAGGCCGGCA‑TGGATATGGGTCTACGGAGATCGGGAGCCACAGTGTACGCCGGAGGGAACGTCaaa > 2:787707/1‑132 (MQ=255)
aTGCCCGTCGCCGACATCCAGCGTGCCGGTTTCATAGGGCTCGATTTCGGGATAGAGGGTGCGCAAGGCCGGCA‑TGGATATGGGTCTACGGAGAt < 1:804882/95‑1 (MQ=255)
aTGCCCGTCGCCGACATCCAGCGTGCCGGTTTCATAGGGCTCGATTTCGGGATAGAGGGTGCGCAAGGCCGGCA‑TGGATATGGGTCTACGGAGAt > 2:804882/1‑95 (MQ=255)
aTGCCCGTCGCCGACATCCAGCGTGCCGGTTTCATAGGGCTCGATTTCGGGATAGAGGGTGCGCAAGGCCGGCA‑TGGATATGGGTCTACGGAGATCGGGAGCCACAGTGTACGCCGGAGGGAACGTCAAACAGt < 2:236878/134‑1 (MQ=255)
gTCGCCGACATCCAGCGTGCCGGTTTCATAGGGCTCGATTTCGGGATAGAGGGTGCGCAAGGCCGGCATT‑GATATGGGTCTACGGAGATCGGGAGc < 2:588959/96‑1 (MQ=255)
gTCGCCGACATCCAGCGTGCCGGTTTCATAGGGCTCGATTTCGGGATAGAGGGTGCGCAAGGCCGGCA‑TGGATATGGGTCTACGGAGATCg < 2:267836/91‑1 (MQ=255)
gTCGCCGACATCCAGCGTGCCGGTTTCATAGGGCTCGATTTCGGGATAGAGGGTGCGCAAGGCCGGCA‑TGGATATGGGTCTACGGAGATCg > 1:267836/1‑91 (MQ=255)
gTCGCCGACATCCAGCGTGCCGGTTTCATAGGGCTCGATTTCGGGATAGAGGGTGCGCAAGGCCGGCA‑TGGATATGGGTCTACGGAGATCGGGAGc > 1:588959/1‑96 (MQ=255)
gTCGCCGACATCCAGCGTGCCGGTTTCATAGGGCTCGATTTCGGGATAGAGGGTGCGCAAGGCCGGCA‑TGGATATGGGTCTACGGAGATCGGGAGCCACAGTGTACGc < 1:649153/108‑1 (MQ=255)
gTCGCCGACATCCAGCGTGCCGGTTTCATAGGGCTCGATTTCGGGATAGAGGGTGCGCAAGGCCGGCA‑TGGATATGGGTCTACGGAGATCGGGAGCCACAGTGTACGc > 2:649153/1‑108 (MQ=255)
aCATCCAGCGTGCCGGTTTCATAGGGCTCGATTTCGGGATAGAGGGTGCGCAAGGCCGGCA‑TGGATATGGGTCTACGGAGATCGGGAGCCACAGTGTACGCCg < 1:308331/103‑1 (MQ=255)
aCATCCAGCGTGCCGGTTTCATAGGGCTCGATTTCGGGATAGAGGGTGCGCAAGGCCGGCA‑TGGATATGGGTCTACGGAGATCGGGAGCCACAGTGTACGCCg > 2:308331/1‑103 (MQ=255)
gCCGGTTTCATAGGGCTCGATTTCGGGATAGAGGGTGCGCAAGGCCGGCA‑TGGATATGGGTCTACGGAGATCGGGAGCCATAGTGTACGCCGGAGGGAACGTCAAACAGTAGCCCGCCCCTTAACCGGCg > 1:862816/1‑130 (MQ=255)
gCCGGTTTCATAGGGCTCGATTTCGGGATAGAGGGTGCGCAAGGCCGGCA‑TGGATATGGGTCTACGGAGATCGGGAGCCATAGTGTACGCCGGAGGGAACGTCAAACAGTAGCCCGCCCCTTAACCGGCg < 2:862816/130‑1 (MQ=255)
ggTTTCATAGGGCTCGATTTCGGGATAGAGGGTGCGCAAGGCCGGCA‑TGGATATGGGTCTACGgaga > 1:591915/1‑67 (MQ=255)
ggTTTCATAGGGCTCGATTTCGGGATAGAGGGTGCGCAAGGCCGGCA‑TGGATATGGGTCTACGgaga < 2:591915/67‑1 (MQ=255)
gggCTCGATTTCGGGATAGAGGGTGCGCAAGGCCGGCA‑TGGATATGGGTCTACGGAGATCGGGAGCCACAGTGTACGCCGGAGGGAACGTCAAACAGTAGCCCGCCCCTTAACCGGCGAACACCGCCTTCAcc < 2:1123898/133‑1 (MQ=255)
gATTTCGGGATAGAGGGTGCGCAAGGCCGGCA‑TGGATATGGGTCTACGGAGATCGGGa < 1:246319/58‑1 (MQ=255)
gATTTCGGGATAGAGGGTGCGCAAGGCCGGCA‑TGGATATGGGTCTACGGAGATCGGGa > 2:246319/1‑58 (MQ=255)
aTTTCGGGATAGAGGGTGCGCAAGGCCGGCA‑TGGATATGGGTCTACGgaga > 1:797366/1‑51 (MQ=39)
aTTTCGGGATAGAGGGTGCGCAAGGCCGGCA‑TGGATATGGGTCTACGgaga < 2:797366/51‑1 (MQ=39)
aTTTCGGGATAGAGGGTGCGCAAGGCCGGCA‑TGGATATGGGTCTACGGAGATCGGGa < 2:395659/57‑1 (MQ=255)
aTTTCGGGATAGAGGGTGCGCAAGGCCGGCA‑TGGATATGGGTCTACGGAGATCGGGa > 1:395659/1‑57 (MQ=255)
aTTTCGGGATAGAGGGTGCGCAAGGCCGGCA‑TGGATATGGGTCTACGGAGATCGGGAGc < 1:263462/59‑1 (MQ=255)
aTTTCGGGATAGAGGGTGCGCAAGGCCGGCA‑TGGATATGGGTCTACGGAGATCGGGAGc > 2:263462/1‑59 (MQ=255)
aTTTCGGGATAGAGGGTGCGCAAGGCCGGCA‑TGGATATGGGTCTACGGAGATCGGGAGCCACAGTGTACGCCGGAGGGaa < 2:91830/80‑1 (MQ=255)
aTTTCGGGATAGAGGGTGCGCAAGGCCGGCA‑TGGATATGGGTCTACGGAGATCGGGAGCCACAGTGTACGCCGGAGGGaa > 1:91830/1‑80 (MQ=255)
aTTTCGGGATAGAGGGTGCGCAAGGCCGGCA‑TGGATATGGGTCTACGGAGATCGGGAGCCACAGTGTACGCCGGAGGGAACGTCAAACAGt < 1:1019147/91‑1 (MQ=255)
aTTTCGGGATAGAGGGTGCGCAAGGCCGGCA‑TGGATATGGGTCTACGGAGATCGGGAGCCACAGTGTACGCCGGAGGGAACGTCAAACAGt > 2:1019147/1‑91 (MQ=255)
aTTTCGGGATAGAGGGTGCGCAAGGCCGGCA‑TGGATATGGGTCTACGGAGATCGGGAGCCACAGTGTACGCCGGAGGGAACGTCAAACAGTAGCCCGCCCCTTAACCGGCGa > 1:424655/1‑112 (MQ=255)
aTTTCGGGATAGAGGGTGCGCAAGGCCGGCA‑TGGATATGGGTCTACGGAGATCGGGAGCCACAGTGTACGCCGGAGGGAACGTCAAACAGTAGCCCGCCCCTTAACCGGCGa < 2:424655/112‑1 (MQ=255)
aTTTCGGGATAGAGGGTGCGCAAGGCCGGCA‑TGGATATGGGTCTACGGAGATCGGGAGCCACAGTGTACGCCGGAGGGAACGTCAAACAGTAGCCCGCCCCTTAACCGGCGAACACCGCCTTCACCCAGTCCCt > 1:424822/1‑134 (MQ=255)
cgcAAGGCCGGCA‑TGGATATGGGTCTACGGAGATCGGGAGCCACAGTGTACGCCGGAGGGAACGTCAAACAGt < 2:1089145/73‑1 (MQ=255)
cgcAAGGCCGGCA‑TGGATATGGGTCTACGGAGATCGGGAGCCACAGTGTACGCCGGAGGGAACGTCAAACAGt > 1:1089145/1‑73 (MQ=255)
cAAGGCCGGCA‑TGGATATGGGTCTACGGAGATCGGGAGCCACAGTGTACGCCGGAGGGAACGTCAAACAGTAGCCCGCCCCTTAACCGGCGa < 2:499797/92‑1 (MQ=255)
cAAGGCCGGCA‑TGGATATGGGTCTACGGAGATCGGGAGCCACAGTGTACGCCGGAGGGAACGTCAAACAGTAGCCCGCCCCTTAACCGGCGa > 1:499797/1‑92 (MQ=255)
aaGGCCGGCA‑TGGATATGGGTCTACGGAGATCGGGAGCCACAGTGTACGCCGGAGGGAACGTCAAACAGTAGCCCGCCCCTTAACCGGCGa > 2:814146/1‑91 (MQ=255)
aaGGCCGGCA‑TGGATATGGGTCTACGGAGATCGGGAGCCACAGTGTACGCCGGAGGGAACGTCAAACAGTAGCCCGCCCCTTAACCGGCGa < 1:814146/91‑1 (MQ=255)
aaGGCCGGCA‑TGGATATGGGTCTACGGAGATCGGGAGCCACAGTGTACGCCGGAGGGAACGTCAAACAGTAGCCCGCCCCTTAACCGGCGAACACCGCCTTCACCCAGTCCCTCAGCAACCGCGTGGCGGGACg > 2:727482/1‑134 (MQ=255)
aGGCCGGCA‑TGGATATGGGTCTACGGAGATCGGGAGCCACAGTGTACGCCGGAGGGAACGTCAAACAGTAGCCCGCCCCTTAACCGGCGAACACCGCCTTCACCCAGTCCCTCAGCAACCGCGTGGCGGGACGc > 1:954680/1‑134 (MQ=255)
|
CGGCTTGGCGCCGGGCGTGCCCACACGCTCGTAGTAGATGACATGCCCGTCGCCGACATCCAGCGTGCCGGTTTCATAGGGCTCGATTTCGGGATAGAGGGTGCGCAAGGCCGGCA‑T‑GATATGGGTCTACGGAGATCGGGAGCCACAGTGTACGCCGGAGGGAACGTCAAACAGTAGCCCGCCCCTTAACCGGCGAACACCGCCTTCACCCAGTCCCTCAGCAACCGCGTGGCGGGACGC > AM260480/2843463‑2843702
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
BRESEQ :: bam2aln output
CCACCGGGCCCGCCGTGCAGGAACACCGCCGGCTTGGCGCCGGGCGTGCCCACACGCTCGTAGTAGATGACATGCCCGTCGCCGACATCCAGCGTGCCGGTTTCATAGGGCTCGATTTCGGGATAGAGGGTGCGCAAGGCCGGCAT‑GATATGGGTCTACGGAGATCGGGAGCCACAGTGTACGCCGGAGGGAACGTCAAACAGTAGCCCGCCCCTTAACCGGCGAACACCGCCTTCACCCAGTCCCTCAGCAACCGCGTGGCGGGACGCTCCGCGGCGCGCTCGCTGTAGACAAG > AM260480/2843434‑2843728
|
gagatgtgtataagagaCAGGAACACCGCCGGCTTGGCGCCGGGCGTGCCCACACGCTCGTAGTAGATGACATGCCCGTCGCCGACATCCAGCGTGCCGGTTTCATAGGGCTCGATTTCGGGATAGAGGGTGCGCAAGGCCGGCAT‑Ggat < GWNJ‑0478:712:GW2002102894th:2:1210:2698:41342/133‑4 (MQ=60)
CCACCGGGCCCGCCGTGCAGGAACACCGCCGGCTTGGCGCCGGGCGTGCCCACACGCTCGTAGTAGATGACATGCCCGTCGCCGACATCCAGCGTGCCGGTTTCATAGGGCTCGATTTCGGGATAGAGGGTGCGCAAGGCCGGCAT‑Ggat < GWNJ‑0478:712:GW2002102894th:2:2209:18331:78995/150‑4 (MQ=60)
gtataagagacagGTGCAGGAACACCGCCGGCTTGGCGCCGGGCGTGCCCACACGCTCGTAGTAGATGACATGCCCGTCGCCGACATCCAGCGTGCCGGTTTCATAGGGCTCGATTTCGGGATAGAGGGTGCGCAAGGCCGGCATGGATA < GWNJ‑0478:712:GW2002102894th:2:2212:11677:54136/137‑1 (MQ=60)
agaagacggcatacgagattagcgagtgtctcgtgggctcggagatgtgtataagagacagGTAGATGACATGCCCGTCGCCGACATCCAGCGTGCCGGTTTCATAGGGCTCGATTTCGGGATAGAGGGTGCGCAAGGCCGGCATGGATA < GWNJ‑0478:712:GW2002102894th:2:2209:12412:7133/89‑1 (MQ=60)
CACCGGGCCCGCCGTGCAGGAACACCGCCGGCTTGGCGCCGGGCGTGCCCACACGCTCGTAGTAGATGACATGCCCGTCGCCGACATCCAGCGTGCCGGTTTCATAGGGCTCGATTTCGGGATAGAGGGTGCGCAAGGCCGGCATGGATA < GWNJ‑0478:712:GW2002102894th:2:2215:10320:66437/150‑1 (MQ=60)
gatttttttgtgttttttttttttttttcaagcagaagacggcatacgagattagcgagtgtctcgtgggctcggagatgtgtataagagacaGGTTTCATAGGGCTCGATTTCGGGATAGAGGGTGCGCAAGGCCGGCATGGATATGGG < GWNJ‑0478:712:GW2002102894th:2:2205:11612:24288/57‑1 (MQ=60)
GGGCCCGCCGTGCAGGAACACCGCCGGCTTGGCGCCGGGCGTGCCCACACGCTCGTAGTAGATGACATGCCCGTCGCCGACATCCAGCGTGCCGGTTTCATAGGGCTCGATTTCGGGATAGAGGGTGCGCAAGGCCGGCATGGATATGGG < GWNJ‑0478:712:GW2002102894th:2:1102:7435:87141/150‑1 (MQ=60)
ttgttttttttttcaagcagaagacggcatacgagattagcgagtgtctcgtgggctcggagatgtgtataagagacagCATCCAGCGTGCCGGTTTCATAGGGCTCGATTTCGGGATAGAGGGTGCGCAAGGCCGGCATGGATATGGG < GWNJ‑0478:712:GW2002102894th:2:1109:3958:90141/70‑1 (MQ=60)
gctttatctttttttttttcaagcagaagacggcatacgagattagcgagtgtctcgtgggcttcggagatgtgtataagagacaGGTTTCATAGGGCTCGATTTCGGGATAGAGGGTGCGCAAGGCCGGCATGGATATGGGTCTACGG < GWNJ‑0478:712:GW2002102894th:2:1104:10879:39439/64‑1 (MQ=60)
GTGCAGGAACACCGCCGGCTTGGCGCCGGGCGTGCCCACACGCTCGTAGTAGATGACATGCCCGTCGCCGACATCCAGCGTGCCGGTTTCATAGGGCTCGATTTCGGGATAGAGGGTGCGCAAGGCCGGCATGGATATGGGTCTACGG > GWNJ‑0478:712:GW2002102894th:2:2201:10593:50114/1‑148 (MQ=60)
ggctcggagatgtgtataagagacaGTGCCCACACGCTCGTAGTAGATGACATGCCCGTCGCCGACATCCAGCGTGCCGGTTTCATAGGGCTCGATTTCGGGATAGAGGGTGCGCAAGGCCGGCATGGATATGGGTCTACGGAGATCGGG < GWNJ‑0478:712:GW2002102894th:2:2102:13886:23929/125‑1 (MQ=60)
ggctcggagatgtgtataagagacaGTGCCCACACGCTCGTAGTAGATGACATGCCCGTCGCCGACATCCAGCGTGCCGGTTTCATAGGGCTCGATTTCGGGATAGAGGGTGCGCAAGGCCGGCATGGATATGGGTCTACGGAGACCGGG < GWNJ‑0478:712:GW2002102894th:2:1213:17377:61169/125‑1 (MQ=60)
CCGCCGGCTTGGCGCCGGGCGTGCCCACACGCTCGTAGTAGATGACATGCCCGTCGCCGACATCCAGCGTGCCGGTTTCATAGGGCTCGATTTCGGGATAGAGGGTGCGCAAGGCCGGCATGGATATGGGTCTACGGAGATCGGGAGCC > GWNJ‑0478:712:GW2002102894th:2:2216:5911:9780/1‑149 (MQ=60)
gctcggagatgtgtataagagacaGCTCGTAGTAGATGACATGCCCGTCGCCGACATCCAGCGTGCCGGTTTCATAGGGCTCGATTTCGGGATAGAGGGTGCGCAAGGCCGGCATGGATATGGGTCTACGGAGATCGGGAGCCACAGTGT < GWNJ‑0478:712:GW2002102894th:2:2115:18839:46863/126‑1 (MQ=60)
cagaagacggcatacgagattagcgagtgtctcgtgggctcggagatgtgtataagagacagGGTTTCATAGGGCTCGATTTCGGGATAGAGGGTGCGCAAGGCCGGCATGGATATGGGTCTACGGAGATCGGGAGCCACAGTGTACGCC < GWNJ‑0478:712:GW2002102894th:2:1114:16274:8941/88‑1 (MQ=60)
agaagacggcatacgagattagcgagtgtctcgtgggctcggagatgtgtataagagacaGGTTTCATAGGGCTCGATTTCGGGATAGAGGGTGCGCAAGGCCGGCATGGATATGGGTCTACGGAGATCGGGAGCCACAGTGTACGCCGG < GWNJ‑0478:712:GW2002102894th:2:1114:7428:93698/90‑1 (MQ=60)
ataagagacagGTAGATGACATGCCCGTCGCCGACATCCAGCGTGCCGGTTTCATAGGGCTCGATTTCGGGATAGAGGGTGCGCAAGGCCGGCATGGATATGGGTCTACGGAGATCGGGAGCCACAGTGTACGCCGGAGGGAACGTCAA < GWNJ‑0478:712:GW2002102894th:2:2104:19751:82047/138‑1 (MQ=60)
atgtgtataagagacaGACATGCCCGTCGCCGACATCCAGCGTGCCGGTTTCATAGGGCTCGATTTCGGGATAGAGGGTGCGCAAGGCCGGCATGGATATGGGTCTACGGAGATCGGGAGCCACAGTGTACGCCGGAGGGAACGTCAAAC < GWNJ‑0478:712:GW2002102894th:2:1203:12852:64450/134‑1 (MQ=60)
TAGTAGATGACATGCCCGTCGCCGACATCCAGCGTGCCGGTTTCATAGGGCTCGATTTCGGGATAGAGGGTGCGCAAGGCCGGCATGGATATGGGTCTACGGAGATCGGGAGCCACAGTGTACGCCGGAGGGAACGTCAAACAGTctgtc > GWNJ‑0478:712:GW2002102894th:2:1204:16789:101155/1‑145 (MQ=60)
GTAGATGACATGCCCGTCGCCGACATCCAGCGTGCCGGTTTCATAGGGCTCGATTTCGGGATAGAGGGTGCGCAAGGCCGGCATGGATATGGGTCTACGGAGATCGGGAGCCACAGTGTACctgtctcttatacacatctccgagcccac > GWNJ‑0478:712:GW2002102894th:2:1116:3349:16019/1‑121 (MQ=60)
GTAGATGACATGCCCGTCGCCGACATCCAGCGTGCCGGTTTCATAGGGCTCGATTTCGGGATAGAGGGTGCGCAAGGCCGGCATGGATATGGGTCTACGGAGATCGGGAGCCACAGTGTACGCCGGctgtctcttatacacatctccg > GWNJ‑0478:712:GW2002102894th:2:2101:21255:99204/1‑126 (MQ=60)
gtgggctcggagatgtgtataagagacaGGTTTCATAGGGCTCGATTTCGGGATAGAGGGTGCGCAAGGCCGGCATGGATATGGGTCTACGGAGATCGGGAGCCACAGTGTACGCCGGAGGGAACGTCAAACAGTAGCCCGCCCCTTAAC < GWNJ‑0478:712:GW2002102894th:2:2211:10594:80440/122‑1 (MQ=60)
GCCGACATCCAGCGTGCCGGTTTCATAGGGCTCGATTTCGGGATAGAGGGTGCGCAAGGCCGGCATGGATATGGGTCTACGGAGATCGGGAGCCATAGTGTACGCCGGAGGGAACGTCAAACAGTAGCCCGCCCCTTAACCGGCGAACAC > GWNJ‑0478:712:GW2002102894th:2:2201:12982:52256/1‑150 (MQ=60)
GACATCCAGCGTGCCGGTTTCATAGGGCTCGATTTCGGGATAGAGGGTGCGCAAGGCCGGCATGGATATGGGTCTACGGAGATCGGGAGCCACAGTGctgtctcttatacacatctccgagcccacgagacactcgctaatctcgtatgc > GWNJ‑0478:712:GW2002102894th:2:2103:3741:19546/1‑97 (MQ=60)
GACATCCAGCGTGCCGGTTTCATAGGGCTCGATTTCGGGATAGAGGGTGCGCAAGGCCGGCAT‑Ggactgtctcttatacacatctccgagcccacgagacactcgctaatctcgtatgccgtcttctgcttgaaaaaaaaaaaatatagg > GWNJ‑0478:712:GW2002102894th:2:1207:3310:2310/1‑64 (MQ=60)
gtgggctcggagatgtgtataagagacaGGGATAGAGGGTGCGCAAGGCCGGCATGGATATGGGTCTACGGAGATCGGGAGCCACAGTGTACGCCGGAGGGAACGTCAAACAGTAGCCCGCCCCTTAACCGGCGAACACCGCCTTCACCC < GWNJ‑0478:712:GW2002102894th:2:2115:4263:91294/122‑1 (MQ=60)
agtttagcgagtgtctcgtgggctcggagatgtgtataagagacaggcatgGATATGGGTCTACGGAGATCGGGAGCCACAGTGTACGCCGGAGGGAACGTCAAACAGTAGCCCGCCCCTTAACCGGCGAACACCGCCTTCACCCAGTCC < GWNJ‑0478:712:GW2002102894th:2:1204:20925:18786/99‑1 (MQ=60)
acgagattagcgagtgtctcgtgggctcggagatgtgtataagagacagGATATGGGTCTACGGAGATCGGGAGCCACAGTGTACGCCGGAGGGAACGTCAAACAGTAGCCCGCCCCTTAACCGGCGAACACCGCCTTCACCCAGTCCC < GWNJ‑0478:712:GW2002102894th:2:1206:9856:15469/100‑1 (MQ=60)
GTTTCATAGGGCTCGATTTCGGGATAGAGGGTGCGCAAGGCCGGCATGGATATGGGTCTACGGAGATCGGGAGCCACAGTGctgtctcttatacacatctccgagcccacgagacactcgctaatctcgtatgccgtcttctgcttgaa > GWNJ‑0478:712:GW2002102894th:2:2115:15978:9926/1‑81 (MQ=60)
GTTTCATAGGGCTCGATTTCGGGATAGAGGGTGCGCAAGGCCGGCATGGATATGGGTCTACGGAGATCGGGAGCCACAGTGTACGCCctgtctcttatacacatctccgagcccacgagacactcgctaatctcgtatgccgtcttctgc > GWNJ‑0478:712:GW2002102894th:2:1206:3103:89154/1‑87 (MQ=60)
GTTTCATAGGGCTCGATTTCGGGATAGAGGGTGCGCAAGGCCGGCATGGATATGGGTCTACGGAGATCGGGAGCCACAGTGTACGCCGGAGGGAACGTCAAACAGTAGCCCtgtctcttatacacatctccgagcccacgagacactcgc > GWNJ‑0478:712:GW2002102894th:2:1107:10181:71624/1‑111 (MQ=60)
GTTTCATAGGGCTCGATTTCGGGATAGAGGGTGCGCAAGGCCGGCATGGATATGGGTCTACGGAGATCGGGAGCCACAGTGTACGCCGGAGGGAACGTCAAACAGTAGCCCGCCCCTTAACCGGCGAACACCGCCTTCACCCctgtctct > GWNJ‑0478:712:GW2002102894th:2:1209:5248:47790/1‑142 (MQ=60)
GTTTCATAGGGCTCGATTTCGGGATAGAGGGTGCGCAAGGCCGGCATGGATATGGGTCTACGGAGATCGGGAGCCACAGTGTACGCCGGAGGGAACGTCAAACAGTAGCCCGCCCCTTAACCGGCGAACACCGCCTTCACCCAGTCCCTC > GWNJ‑0478:712:GW2002102894th:2:1209:20387:48706/1‑150 (MQ=60)
CGATTTCGGGATAGAGGGTGCGCAAGGCCGGCAT‑Ggatctgtctcttatacacatctccgagcccacgagacactcgctaatctcgtatgccgtcttctgcttgaaaaaaaaaaaaaaacaccgactaaacacatgaacaggccaacga > GWNJ‑0478:712:GW2002102894th:2:1114:9258:50834/1‑35 (MQ=60)
TCGGGATAGAGGGTGCGCAAGGCCGGCATGGATATGGGTCTACGGAGATCGGGAGCCACAGTGTACGCCGGAGGGAACGTCAAACAGTAGCCCGCCCCTTAACCtgtctcttatacacatctccgagcccacgagacactcgctaatctc > GWNJ‑0478:712:GW2002102894th:2:2214:15562:41301/1‑104 (MQ=60)
GGGATAGAGGGTGCGCAAGGCCGGCATGGATATGGGTCTACGGAGATCGGGAGCCACAGTGTACGCCGGAGGGAACGTCAAACAGTAGCCCGCCCCTTAACCGGCGAACACCGCCTTCACCCctgtctcttatacacatctccgagccc > GWNJ‑0478:712:GW2002102894th:2:1212:12602:34456/1‑122 (MQ=60)
GATAGAGGGTGCGCAAGGCCGGCATGGATATGGGTCTACGGAGATCGGGAGCCACAGTGTACGCCGGAGGGAACGTCAAACAGTAGCCCGCCCCTTAACCGGCGAACACCGCCTTCACCCAGTCCCTCAGCAACCGCGTGGCGGGACGCT > GWNJ‑0478:712:GW2002102894th:2:2206:15161:12110/1‑150 (MQ=60)
GCCGGCATGGATATGGGTCTACGGAGATCGGGAGCCACAGTGTACGCCGGAGGGAACGTCAAACAGTAGCCCGCCCCTTAACCGGCGAACACCGCCTTCACCCAGTCCCTCAGCAACCGCGTGGCGGGACGCTCCGCGGCGCGCTCGCTG > GWNJ‑0478:712:GW2002102894th:2:1212:9878:72136/1‑150 (MQ=60)
CGGCATGGATATGGGTCTACGGAGATCGGGAGCCACAGTGTACGCCGGAGGGAACGTCAAACAGTAGCCCGCCCCTTAACCGGCGAACACCGCCctgtctcttatacacatctccgagcccacgagacactcgctaatctcgtatgccgt > GWNJ‑0478:712:GW2002102894th:2:2111:19872:41862/1‑94 (MQ=60)
G‑GATATGGGTCTACGGAGATCGGGAGCCACAGTGTACGCCGGAGGGAACGTCAAACAGTAGCCCGCCCCTTAACCtgtctcttatacacatctccgagcccacgagacactcgctaatctcgtatgccgtcttctgcttgaaaaaaaaa > GWNJ‑0478:712:GW2002102894th:2:1215:2032:89984/1‑75 (MQ=60)
G‑GATATGGGTCTACGGAGATCGGGAGCCACAGTGTACGCCGGAGGGAACGTCAAACAGTAGCCCGCCCCTTAACCGGCGAACACCGCCTTCACCCAGTCCCTCAGCctgtctcttatacacatctccgagcccacgagacactcgctaat > GWNJ‑0478:712:GW2002102894th:2:2213:3987:34217/1‑106 (MQ=60)
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CCACCGGGCCCGCCGTGCAGGAACACCGCCGGCTTGGCGCCGGGCGTGCCCACACGCTCGTAGTAGATGACATGCCCGTCGCCGACATCCAGCGTGCCGGTTTCATAGGGCTCGATTTCGGGATAGAGGGTGCGCAAGGCCGGCAT‑GATATGGGTCTACGGAGATCGGGAGCCACAGTGTACGCCGGAGGGAACGTCAAACAGTAGCCCGCCCCTTAACCGGCGAACACCGCCTTCACCCAGTCCCTCAGCAACCGCGTGGCGGGACGCTCCGCGGCGCGCTCGCTGTAGACAAG > AM260480/2843434‑2843728
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| Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
