Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F1 I1 R1
|
154 |
26.6 |
2069536 |
90.2% |
1866721 |
108.9 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
AM260479 |
1,841,200 |
+G |
intergenic (‑2736/‑2680) |
h16_A1684 ← / → ycaC |
conserved hypothetical protein/YcaC related amidohydrolase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | AM260479 | 1,841,197 | 1 | . | G | 100.0%
| 78.6
/ NA
| 21 | intergenic (‑2733/‑2683) | h16_A1684/ycaC | conserved hypothetical protein/YcaC related amidohydrolase |
| Reads supporting (aligned to +/- strand): ref base . (0/0); new base G (11/10); total (11/10) |
CGCCCAGCATCCGCCCTCGATGATCCGGCCGGTACGGCAAAGCGCATCGTGGCCGCTGTAGGCAGCCGCCTGCAGGTAGCCATGATAGTCGCGCAGGAAGTTCA‑GGGATGGGCGCCGGCGCGGGATTCCGCGAACTCGAACAGCACCGCTGGCTGGTGGTCGACCCATAATCCGTTCTCCTGCCGCAGCCCGGCCACGAGGTAGCCCCATAGATGGGCC > AM260479/1841094‑1841312
|
cgcCCAGCATCCGCCCTCGATGATCCGGCCGGTACGGCAAATCGCATCGTGGCCGCTGTAGGCAGCCGCCTGCAGGTAGCCATGATAGTCGCGCAGGAAGTTCAGGGGATGGGCGCCGGCGCGGGATTCcgcg > 2:992947/1‑133 (MQ=255)
tCCGCCCTCGATGATCCGGCCGGTACGGCAAAGCGCATCGTGGCCGCTGTAGGCAGCCGCCTGCAGGTAGCCATGATAGTCGCGCAGGAAGTTCAGGGGATGGGCGCCGGCGCGGGATTCCGCGAACTCGaa > 1:171071/1‑132 (MQ=255)
cGATGATCCGGCCGGTACGGCAAAGCGCATCGTGGCCGCTGTAGGCAGCCGCCTGCAGGTAGCCATGATAGTCGCGCAGGAAGTTCAGGGGATGGGCGCCGGCGCGGGATTCCGCGAACTCGAACAGCACCgct > 1:598580/1‑134 (MQ=255)
ccGCTGTAGGCAGCCGCCTGCAGGTAGCCATGATAGTCGCGCAGGAAGTTCAGGGGATGGGCGCCGGCGCGGGATTCCGCGAACTCGAACAGCACCGCTGGCTGGTGGTCGACCCATAATCCGTTCTCCTGCCg < 1:992947/134‑1 (MQ=255)
tGTAGGCAGCCGCCTGCAGGTAGCCATGATAGTCGCGCAGGAAGTTCAGGGGATGGGCGCCGGCGCGGGATTCCGCGAACTCGAACAGCACCGCTGGCTGGTGGTCGACCCATAATCCGTTCTCCTGCCGCAGc > 1:151974/1‑134 (MQ=255)
aGGCAGCCGCCTGCAGGTAGCCATGATAGTCGCGCAGGAAGTTCAGGGGATGGGCGCCGGCGCGGGATTCCGCGAACTCGAACAGCACCGCTGGCTGGTGGTCGACCCATAATCCGTTCTCCTGCCGCAGCCCg > 2:181719/1‑134 (MQ=255)
aGGCAGCCGCCTGCAGGTAGCCATGATAGTCGCGCAGGAAGTTCAGGGGATGGGCGCCGGCGCGGGATTCCGCGAACTCGAACAGCACCGCTGGCTGGTGGTCGACCCATAATCCGTTCTCCTGCCGCAGCCCg > 2:233159/1‑134 (MQ=255)
ggCAGCCGCCTGCAGGTAGCCATGATAGTCGCGCAGGAAGTTCAGGGGATGGGCGCCGGCGCGGGATTCCGCGAACTCGAACAGCACCGCTGGCTGGTGGTCGACCCATAATCCGTTCTCCTGCCGCAGCCCg < 2:171071/133‑1 (MQ=255)
gccgccTGCAGGTAGCCATGATAGTCGCGCAGGAAGTTCAGGGGATGGGCGCCGGCGCGGGATTCCGCGAACTCGAACAGCACCGCTGGCTGGTGGTCGACCCATAATCCGTTCTCCTGCCGCAGCCCGGCCAc < 2:151974/134‑1 (MQ=255)
ccgccTGCAGGTAGCCATGATAGTCGCGCAGGAAGTTCAGGGGATGGGCGCCGGCGCGGGATTCCGCGAACTCGAACAGCACCGCTGGCTGGTGGTCGACCCATAATCCGTTCTCCTGCCGCAGCCCGGCCACg < 1:181719/134‑1 (MQ=255)
ccgccTGCAGGTAGCCATGATAGTCGCGCAGGAAGTTCAGGGGATGGGCGCCGGCGCGGGATTCCGCGAACTAGAACAGCACCGCTGGCTGGTGGTCGACCCATAATCCGTTCTCCTGCCGCAGCCCGGCCACg < 1:233159/134‑1 (MQ=255)
cTGCAGGTAGCCATGATAGTCGCGCAGGAAGTTCAGGGGATGGGCGCCGGCGCGGGATTCCGCGAACTCGAACAGCACCGCTGGCTGGTGGTCGACCCATAATCCGTTCTCCTGCCGCAGCCCGGCCACGAgg > 2:588145/1‑133 (MQ=255)
aTGATAGTCGCGCAGGAAGTTCAGGGGATGGGCGCCGGCGCGGGATTCCGCGAACTCGAACAGCACCGCTGGCTGGTGGTCGACCCATAATCCGTTCTCCTGCCGCAGCCCGGCCACGAGGTAGCCCCATAGAt < 1:588145/134‑1 (MQ=255)
gcgcAGGAAGTTCAGGGGATGGGCGCCGGCGCGGGATTCCGCGAACTCGAACAGCACCGCTGGCTGGTGGTCGACCCATAATCCGTTCTCCTGCCGCAGCCCGGCCACGAGGTAGCCCCATAGATGGGcc > 1:150057/1‑130 (MQ=255)
gcgcAGGAAGTTCAGGGGATGGGCGCCGGCGCGGGATTCCGCGAACTCGAACAGCACCGCTGGCTGGTGGTCGACCCATAATCCGTTCTCCTGCCGCAGCCCGGCCACGAGGTAGCCCCATAGATGGGcc < 2:150057/130‑1 (MQ=255)
gcAGGAAGTTCAGGGGATGGGCGCCGGCGCGGGATTCCGCGAACTCGAACAGCACCGCTGGCtggtg > 2:993220/1‑67 (MQ=255)
gcAGGAAGTTCAGGGGATGGGCGCCGGCGCGGGATTCCGCGAACTCGAACAGCACCGCTGGCtggtg < 1:993220/67‑1 (MQ=255)
gcAGGAAGTTCAGGGGATGGGCGCCGGCGCGGGATTCCGCGAACTCGAACAGCACCGCTGGCTGGTGGTCGACCCATAATCCGTTCTCCTGCCGCAGCCCGGCCAc > 2:701363/1‑106 (MQ=255)
gcAGGAAGTTCAGGGGATGGGCGCCGGCGCGGGATTCCGCGAACTCGAACAGCACCGCTGGCTGGTGGTCGACCCATAATCCGTTCTCCTGCCGCAGCCCGGCCAc > 2:723756/1‑106 (MQ=255)
gcAGGAAGTTCAGGGGATGGGCGCCGGCGCGGGATTCCGCGAACTCGAACAGCACCGCTGGCTGGTGGTCGACCCATAATCCGTTCTCCTGCCGCAGCCCGGCCAc < 1:723756/106‑1 (MQ=255)
gcAGGAAGTTCAGGGGATGGGCGCCGGCGCGGGATTCCGCGAACTCGAACAGCACCGCTGGCTGGTGGTCGACCCATAATCCGTTCTCCTGCCGCAGCCCGGCCAc < 1:701363/106‑1 (MQ=255)
|
CGCCCAGCATCCGCCCTCGATGATCCGGCCGGTACGGCAAAGCGCATCGTGGCCGCTGTAGGCAGCCGCCTGCAGGTAGCCATGATAGTCGCGCAGGAAGTTCA‑GGGATGGGCGCCGGCGCGGGATTCCGCGAACTCGAACAGCACCGCTGGCTGGTGGTCGACCCATAATCCGTTCTCCTGCCGCAGCCCGGCCACGAGGTAGCCCCATAGATGGGCC > AM260479/1841094‑1841312
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
BRESEQ :: bam2aln output
GCAATGCGCCCAGCATCCGCCCTCGATGATCCGGCCGGTACGGCAAAGCGCATCGTGGCCGCTGTAGGCAGCCGCCTGCAGGTAGCCATGATAGTCGCGCAGGAAGTTCA‑GGGATGGGCGCCGGCGCGGGATTCCGCGAACTCGAACAGCACCGCTGGCTGGTGGTCGACCCATAATCCGTTCTCCTGCCGCAGCCCGGCCACGAGGTAGCCCCATAGATGGGCCTGCCGCGTGGTGGCGCGCCTGCGCTCGAGTAGTG > AM260479/1841088‑1841346
|
GCAATGCGCCCAGCATCCGCCCTCGATGATCCGGCCGGTACGGCAAAGCGCATCGTGGCCGCTGTAGGCAGCCGCCTGCAGGTAGCCATGATAGTCGCGCAGGAAGTTCAGGGGATGGGCGCCGGCGCGGGATTCCGCGAACTCGAAC > GWNJ‑0478:712:GW2002102894th:2:1113:18009:10529/1‑148 (MQ=60)
CCCAGCATCCGCCCTCGATGATCCGGCCGGTACGGCAAAGCGCATCGTGGCCGCTGTAGGCAGCCGCCTGCAGGTAGCCATGATAGTCGCGCAGGAAGTTCAGGGGATGGGCGCCGGCGCGGGATTCCGCGAACTCGAACAGCACCGCTG > GWNJ‑0478:712:GW2002102894th:2:2107:17303:59218/1‑150 (MQ=60)
gcatacgagatgtagctccgtctcgtgggctcggagatgtgtataagagacagAGCCATGATAGTCGCGCAGGAAGTTCAGGGGATGGGCGCCGGCGCGGGATTCCGCGAACTCGAACAGCACCGCTGGCTGGTGGTCGACCCATAATCC < GWNJ‑0478:712:GW2002102894th:2:2214:16382:73163/97‑1 (MQ=60)
GCGCATCGTGGCCGCTGTAGGCAGCCGCCTGCAGGTAGCCATGATAGTCGCGCAGGAAGTTCAGGGGATGGGCGCCGGCGCGGGATTCCGCGAACTCGAACAGCACCGCTGGCTGGTGGTCGACCCATAATCCGTTCTCCTGCCGCAGCC > GWNJ‑0478:712:GW2002102894th:2:1111:11011:95581/1‑150 (MQ=60)
GCCGCTGTAGGCAGCCGCCTGCAGGTAGCCATGATAGTCGCGCAGGAAGTTCAGGGGATGGGCGCCGGCGCGGGATTCCGCGAACTCGAACAGCACCGCTGGCTGGTGGTCGACCCATAATCCGTTCTCCTGCCGCAGCCCGGCCACGAG < GWNJ‑0478:712:GW2002102894th:2:2214:13314:71737/150‑1 (MQ=60)
gtataagagacagAGCCATGATAGTCGCGCAGGAAGTTCAGGGGATGGGCGCCGGCGCGGGATTCCGCGAACTCGAACAGCACCGCTGGCTGGTGGTCGACCCATAATCCGTTCTCCTGCCGCAGCCCGGCCACGAGGTAGCCCCATAG < GWNJ‑0478:712:GW2002102894th:2:2113:6245:87957/136‑1 (MQ=60)
gtataagagacagAGCCATGATAGTCGCGCAGGAAGTTCAGGGGATGGGCGCCGGCGCGGGATTCCGCGAACTCGAACAGCACCGCTGGCTGGTGGTCGACCCATAATCCGTTCTCCTGCCGCAGCCCGGCCACGAGGTAGCCCCATAG < GWNJ‑0478:712:GW2002102894th:2:2115:17966:21189/136‑1 (MQ=60)
GCCGCCTGCAGGTAGCCATGATAGTCGCGCAGGAAGTTCAGGGGATGGGCGCCGGCGCGGGATTCCGCGAACTCGAACAGCACCGCTGGCTGGTGGTCGACCCATAATCCGTTCTCCTGCCGCAGCCCGGCCACGAGGTAGCCCCATAGA < GWNJ‑0478:712:GW2002102894th:2:1113:19678:71480/150‑1 (MQ=60)
GCCGCCTGCAGGTAGCCATGATAGTCGCGCAGGAAGTTCAGGGGATGGGCGCCGGCGCGGGATTCCGCGAACTAGAACAGCACCGCTGGCTGGTGGTCGACCCATAATCCGTTCTCCTGCCGCAGCCCGGCCACGAGGTAGCCCCATAGA < GWNJ‑0478:712:GW2002102894th:2:1114:3650:66381/150‑1 (MQ=60)
GCCTGCAGGTAGCCATGATAGTCGCGCAGGAAGTTCAGGGGATGGGCGC > GWNJ‑0478:712:GW2002102894th:2:1203:7718:60178/1‑49 (MQ=60)
GTAGCCATGATAGTCGCGCAGGAAGTTCAGGGGATGGGCGCCGGCGCGGGATTCCGCGAACTCGAACAGCACCGCTGGCTGGTGGTCGACCCATAATCCGTTCTCCTGCCGCAGCCCGGCCACGAGGTAGCCCCATAGATGGGCCTGCCG > GWNJ‑0478:712:GW2002102894th:2:1111:9072:84807/1‑150 (MQ=60)
CATGATAGTCGCGCAGGAAGTTCAGGGGATGGGCGCCGGCGCGGGATTCCGCGAACTCGAACAGCACCGCTGGCTGGTGGTCGACCCATAATCCGTTCTCCTGCCGCAGCCCGGCCACGAGGTAGCCCCATAGATGGGCCTGCCGCGTGG < GWNJ‑0478:712:GW2002102894th:2:2104:2342:90806/150‑1 (MQ=60)
CAGGAAGTTCAGGGGATGGGCGCCGGCGCGGGATTCCGCGAACTCGAACAGCACCGCTGGCTGGTGGTCGACCCATAATCCGTTCTCCTGCCGCAGCCCGGCCACGAGGTAGCCCCATAGATGGGCCTGCCGCGTGGTGGCGCGCCctgt > GWNJ‑0478:712:GW2002102894th:2:1214:5121:42001/1‑146 (MQ=60)
cagGGGATGGGCGCCGGCGCGGGATTCCGCGAACTCGAACAGCACCGCTGGCTGGTGGTCGACCCATAATCCGTTCTCCTGCCGCAGCCCGGCCACGAGGTAGCCCCATAGATGGGCCTGCCGCGTGGTGGCGCGCCTGCGCTCGAGTAG < GWNJ‑0478:712:GW2002102894th:2:2209:19138:6138/147‑1 (MQ=60)
G‑GGGATGGGCGCCGGCGCGGGATTCCGCGAACTCGAACAGCACCGCTGGCTGGTGGTCGACCCATAATCCGTTCTCCTGCCGCAGCCCGGCCACGAGGTAGCCCCATAGATGGGCCTGCCGCGTGGTGGCGCGCCTGCGCTCGAGTAGTG > GWNJ‑0478:712:GW2002102894th:2:2215:6180:10080/1‑150 (MQ=60)
|
GCAATGCGCCCAGCATCCGCCCTCGATGATCCGGCCGGTACGGCAAAGCGCATCGTGGCCGCTGTAGGCAGCCGCCTGCAGGTAGCCATGATAGTCGCGCAGGAAGTTCA‑GGGATGGGCGCCGGCGCGGGATTCCGCGAACTCGAACAGCACCGCTGGCTGGTGGTCGACCCATAATCCGTTCTCCTGCCGCAGCCCGGCCACGAGGTAGCCCCATAGATGGGCCTGCCGCGTGGTGGCGCGCCTGCGCTCGAGTAGTG > AM260479/1841088‑1841346
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
