Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F1 I1 R1
|
154 |
26.6 |
2069536 |
90.2% |
1866721 |
108.9 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
AM260479 |
2,517,380 |
G→C |
P77A (CCA→GCC) |
zntA ← |
putative heavy metal transporting P‑type ATPase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | AM260479 | 2,517,380 | 0 | G | C | 100.0%
| 75.3
/ NA
| 20 | P77A (CCA→GCC) | zntA | putative heavy metal transporting P‑type ATPase |
| Reads supporting (aligned to +/- strand): ref base G (0/0); new base C (10/10); total (10/10) |
CGGACAAACTGGGCGCGCAACTCAGGGGTGTCGTAGGCGGCCCAGATCGGTTCGGCCTCGCGACGGGCGTCGTCGTCGATGGGGCGGGCGAAGCGGGCTTCATCCCCGTACAGGTGCGCAAAGCCTGGCGCATAGAGGGTCTGCGCCAATGCCTGGCAACCGCCGCAGCAGAACACCCGGCGGCTGCCGTCCAGCTCGGCGGCAAGCGGCTCGGTGTCCGTGACGGGCAGGCCGCAATG > AM260479/2517253‑2517491
|
cggACAAACTGGGCGCGCAACTCAGGGGTGTCGTAGGCGGCCCAGATCGGTTCGGCCTCGCGACGGGCGTCGTCGTCGATGGGGCGGGCGAAGCGGGCTTCATCCCCGTACAGGTGCGCAAAGCCGGCCGCAtg < 1:168492/134‑2 (MQ=255)
cTCAGGGGTGTCGTAGGCGGCCCAGATCGGTTCGGCCTCGCGACGGGCGTCGTCGTCGATGGGGCGGGCGAAGCGGGCTTCATCCCCGTACAGGTGCGCAAAGCCGGCCGCATGGAGGGTCTGCGCCAATGCCt < 1:101699/134‑1 (MQ=255)
aGGCGGCCCAGATCGGTTCGGCCTCGCGACGGGCGTCGTCGTCGATGGGGCGGGCGAAGCGGGCTTCATCCCCGTACAGGTGCGCAAAGCCGGCCGCATGGAGGGTCTGCGCCAATGCCTGGCAACCGCCgcag > 1:174854/1‑134 (MQ=255)
gCCTCGCGACGGGCGTCGTCGTCGATGGGGCGGGCGGAGCGGGCTTCATCCCCGTACAGGTGCGCAAAGCCGGCCGCATGGAGGGTCTGCGCCAATGCCTGGCAACCGCCGCAGCAGAACACCCGGCGGCTGcc > 2:434920/1‑134 (MQ=255)
gCCTCGCGACGGGCGTCGTCGTCGATGGGGCGGGCGAAGCGGGCTTCATCCCCGTACAGGTGCGCAAAGCCGGCCGCATGGAGGGTCTGCGCCAATGCCTgg < 1:4593/102‑1 (MQ=255)
gCCTCGCGACGGGCGTCGTCGTCGATGGGGCGGGCGAAGCGGGCTTCATCCCCGTACAGGTGCGCAAAGCCGGCCGCATGGAGGGTCTGCGCCAATGCCTgg > 2:4593/1‑102 (MQ=255)
gCCTCGCGACGGGCGTCGTCGTCGATGGGGCGGGCGAAGCGGGCTTCATCCCCGTACAGGTGCGCAAAGCCGGCCGCATGGAGGGTCTGCGCCAATGCCTGGCAACCGCCGCAGCAGAACACCCGGCGGCTGcc > 1:695675/1‑134 (MQ=255)
tcgtcgtcgATGGGGCGGGCGAAGCGGGCTTCATCCCCGTACAGGTGCGCAAAGCCGGCCGCATggag > 1:285651/1‑68 (MQ=255)
tcgtcgtcgATGGGGCGGGCGAAGCGGGCTTCATCCCCGTACAGGTGCGCAAAGCCGGCCGCATggag < 2:285651/68‑1 (MQ=255)
tcgATGGGGCGGGCGGAGCGGGCTTCATCCCCGTACAGGTGCGCAAAGCCGGCCGCATGGAGGGTCTGCGCCAATGCCTGGCAACCGCCGCAGCAGAACACCCGGCGGCTGCCGTCCAGCTCGGCGGCAAGCgg < 1:434920/134‑1 (MQ=255)
ggcgAAGCGGGCTTCATCCCCGTACAGGTGCGCAAAGCCGGCCGCATGGAGGGTCTGCGCCAATGCCTGGCAACCGCCGCAGCAGAACACCCGGCGGCTGCCGTCCAGCTCGGCGGCAAGCGGCTCGGTGTCCg > 1:803383/1‑134 (MQ=255)
aGCGGGCTTCATCCCCGTACAGGTGCGCAAAGCCGGCCGCATGGAGGGTCTGCGCCAATGCCTGGCAACCGCCGCAGCAGAACACCCGGCGGCTGCCGTCCAGCTCGGCGGCAAGCGGCTCGGTGTCCGTGACg > 1:964463/1‑134 (MQ=255)
ggCTTCATCCCCGTACAGGTGCGCAAAGCCGGCCGCATGGAGGGTCTGCGCCAATGCCTGGCAACCGCCGCAGCAGAACACCCGGCGGCTGCCGTCCAGCTCGGCGGCAAGCGGCTCGGTGTCCGTGACGGGCa < 2:803383/134‑1 (MQ=255)
ccGTACAGGTGCGCAAAGCCGGCCGCATGGAGGGTCTGCGCCAATGCCTGGCAACCGCCGCAGCAGAACACCCGGCGGCTGCCGTCCAGCTCGGCGGCAAGCGGCTCGGTGTCCGTGACGGGCAGGCCGCAATg < 2:174854/134‑1 (MQ=255)
cAGGTGCGCAAAGCCGGCCGCATGGAGGGTCTGCGCCAATGCCTGGCAACCGCCGCAGCAGAACACCCGGCGGCTGCCGTCCAGCTcggcgg < 1:661050/92‑1 (MQ=255)
cAGGTGCGCAAAGCCGGCCGCATGGAGGGTCTGCGCCAATGCCTGGCAACCGCCGCAGCAGAACACCCGGCGGCTGCCGTCCAGCTcggcgg > 2:661050/1‑92 (MQ=255)
ggTGCGCAAAGCCGGCCGCATGGAGGGTCTGCGCCAATGCCTGGCAACCGCCGCAGCAGAACACCCGGCGGCTGCCGTCCAGCTCggcggc < 1:464878/91‑1 (MQ=255)
ggTGCGCAAAGCCGGCCGCATGGAGGGTCTGCGCCAATGCCTGGCAACCGCCGCAGCAGAACACCCGGCGGCTGCCGTCCAGCTCggcggc > 2:464878/1‑91 (MQ=255)
gcAAAGCCGGCCGCATGGAGGGTCTGCGCCAATGCCTGGCAACCGCCGCAGCAGAACACCCGGCGGCTGCCGTCCAGCTCGGCGGCAAGc < 1:522649/90‑1 (MQ=255)
gcAAAGCCGGCCGCATGGAGGGTCTGCGCCAATGCCTGGCAACCGCCGCAGCAGAACACCCGGCGGCTGCCGTCCAGCTCGGCGGCAAGc > 2:522649/1‑90 (MQ=255)
|
CGGACAAACTGGGCGCGCAACTCAGGGGTGTCGTAGGCGGCCCAGATCGGTTCGGCCTCGCGACGGGCGTCGTCGTCGATGGGGCGGGCGAAGCGGGCTTCATCCCCGTACAGGTGCGCAAAGCCTGGCGCATAGAGGGTCTGCGCCAATGCCTGGCAACCGCCGCAGCAGAACACCCGGCGGCTGCCGTCCAGCTCGGCGGCAAGCGGCTCGGTGTCCGTGACGGGCAGGCCGCAATG > AM260479/2517253‑2517491
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
BRESEQ :: bam2aln output
GCGGACAAACTGGGCGCGCAACTCAGGGGTGTCGTAGGCGGCCCAGATCGGTTCGGCCTCGCGACGGGCGTCGTCGTCGATGGGGCGGGCGAAGCGGGCTTCATCCCCGTACAGGTGCGCAAAGCCTGGCGCATAGAGGGTCTGCGCCAATGCCTGGCAACCGCCGCAGCAGAACACCCGGCGGCTGCCGTCCAGCTCGGCGGCAAGCGGCTCGGTGTCCGTGACGGGCAGGCCGCAATGGAAGCAGG > AM260479/2517252‑2517499
|
GCGGACAAACTGGGCGCGCAACTCAGGGGTGTCGTAGGCGGCCCAGATCGGTTCGGCCTCGCGACGGGCGTCGTCGTCGATGGGGCGGGCGAAGCGGGCTTCATCCCCGTACAGGTGCGCAAAGCCGGCCGCATGGAGGGTCTGCGCCAA < GWNJ‑0478:712:GW2002102894th:2:1110:3479:95372/150‑1 (MQ=60)
ACTCAGGGGTGTCGTAGGCGGCCCAGATCGGTTCGGCCTCGCGACGGGCGTCGTCGTCGATGGGGCGGGCGAAGCGGGCTTCATCCCCGTACAGGTGCGCAAAGCCGGCCGCATGGAGGGTCTGCGCCAATGCCTGGCAACCGCCGCAGC < GWNJ‑0478:712:GW2002102894th:2:1106:4623:88014/150‑1 (MQ=60)
ACTCAGGGGTGTCGTAGGCGGCCCAGATCGGTTCGGCCTCGCGACGGGCGTCGTCGTCGATGGGGCGGGCGAAGCGGGCTTCATCCCCGTACAGGTGCGCAAAGCCGGCCGCATGGAGGGTCTGCGCCAATGCCTGGCAACCGCCGCAGC > GWNJ‑0478:712:GW2002102894th:2:1113:12354:31976/1‑150 (MQ=60)
gatgtgtataagagacaGGCCCAGATCGGTTCGGCCTCGCGACGGGCGTCGTCGTCGATGGGGCGGGCGAAGCGGGCTTCATCCCCGTACAGGTGCGCAAAGCCGGCCGCATGGAGGGTCTGCGCCAATGCCTGGCAACCGCCGCAGCAG < GWNJ‑0478:712:GW2002102894th:2:1101:10286:35908/133‑1 (MQ=60)
GCCCAGATCGGTTCGGCCTCGCGACGGGCGTCGTCGTCGATGGGGCGGGCGAAGCGGGCTTCATCCCCGTACAGGTGCGCAAAGCCGGCCGCATGGAGGGTCTGCGCCAATGCCTGGCAACCGCCGCAGCAGAACACCCGGCGGCTGCCG > GWNJ‑0478:712:GW2002102894th:2:2113:18263:55583/1‑150 (MQ=60)
GCCTCGCGACGGGCGTCGTCGTCGATGGGGCGGGCGAAGCGGGCTTCATCCCCGTACAGGTGCGCAAAGCCGGCCGCATGGAGGGTCTGCGCCAATGCCTGtctcttatacacatctccgagcccacgagacggagctacatctcgtatg > GWNJ‑0478:712:GW2002102894th:2:1202:6498:16859/1‑101 (MQ=60)
agctccgtctcgtgggctcggagatgtgtataagagacagGTACAGGTGCGCAAAGCCGGCCGCATGGAGGGTCTGCGCCAATGCCTGGCAACCGCCGCAGCAGAACACCCGGCGGCTGCCGTCCAGCTCGGCGGCAAGCGGCTCGGTG < GWNJ‑0478:712:GW2002102894th:2:2113:16405:4034/109‑1 (MQ=60)
gtgggctcggagatgtgtataagagacaGCTTCATCCCCGTACAGGTGCGCAAAGCCGGCCGCATGGAGGGTCTGCGCCAATGCCTGGCAACCGCCGCAGCAGAACACCCGGCGGCTGCCGTCCAGCTCGGCGGCAAGCGGCTCGGTGTC < GWNJ‑0478:712:GW2002102894th:2:1215:19090:8610/122‑1 (MQ=60)
gggctcggagatgtgtataagagacaGGGCTTCATCCCCGTACAGGTGCGCAAAGCCGGCCGCATGGAGGGTCTGCGCCAATGCCTGGCAACCGCCGCAGCAGAACACCCGGCGGCTGCCGTCCAGCTCGGCGGCAAGCGGCTCGGTGT < GWNJ‑0478:712:GW2002102894th:2:2111:2869:52949/123‑1 (MQ=60)
GTCGTCGATGGGGCGGGCGAAGCGGGCTTCATCCCCGTACAGGTGCGCAAAGCCGGCCGCATGGAGGGTCTGCGCCAATGCCTGGCAACCGCCGCAGCAGAACACCCGGCGGCTGCCGTCCAGCTCGGCGGCAAGCGGCTCGGTGTCCGT > GWNJ‑0478:712:GW2002102894th:2:2202:21054:10720/1‑150 (MQ=60)
cgtgggctcggagatgtgtataagagacagTCCCCGTACAGGTGCGCAAAGCCGGCCGCATGGAGGGTCTGCGCCAATGCCTGGCAACCGCCGCAGCAGAACACCCGGCGGCTGCCGTCCAGCTCGGCGGCAAGCGGCTCGGTGTCCGTG < GWNJ‑0478:712:GW2002102894th:2:2101:4759:29319/120‑1 (MQ=60)
GTCGATGGGGCGGGCGGAGCGGGCTTCATCCCCGTACAGGTGCGCAAAGCCGGCCGCATGGAGGGTCTGCGCCAATGCCTGGCAACCGCCGCAGCAGAACACCCGGCGGCTGCCGTCCAGCTCGGCGGCAAGCGGCTCGGTGTCCGTGAC < GWNJ‑0478:712:GW2002102894th:2:1213:17941:41532/150‑1 (MQ=60)
CGATGGGGCGGGCGAAGCGGGCTTCATCCCCGTACAGGTGCGCAAAGCCGGCCGCATGGAGGGTCTGCGCCAATGCCTGGCAACCGCCGCAGCAGAACACCCGGCGGCTGCCGTCCAGCTCGGCGGCAAGCGGCTCGGTGTCCGTGACGG > GWNJ‑0478:712:GW2002102894th:2:2213:18750:15732/1‑150 (MQ=60)
CTTCATCCCCGTACAGGTGCGCAAAGCCGGCCGCATGGAGGGTCTGCGCCAATGCCTGtctcttatacacatctccgagcccacgagacggagctacatctcgtatgccgtcttctgcttgaaaaaaaaaaaaaaaaccatgtaaactct > GWNJ‑0478:712:GW2002102894th:2:2202:4935:79982/1‑58 (MQ=60)
|
GCGGACAAACTGGGCGCGCAACTCAGGGGTGTCGTAGGCGGCCCAGATCGGTTCGGCCTCGCGACGGGCGTCGTCGTCGATGGGGCGGGCGAAGCGGGCTTCATCCCCGTACAGGTGCGCAAAGCCTGGCGCATAGAGGGTCTGCGCCAATGCCTGGCAACCGCCGCAGCAGAACACCCGGCGGCTGCCGTCCAGCTCGGCGGCAAGCGGCTCGGTGTCCGTGACGGGCAGGCCGCAATGGAAGCAGG > AM260479/2517252‑2517499
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
