Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F1 I1 R1
|
154 |
26.6 |
2069536 |
90.2% |
1866721 |
108.9 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
AM260480 |
753,133 |
T→C |
K37E (AAG→GAG) |
h16_B0675 ← |
Acyl‑CoA dehydrogenase,short‑chain specific |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | AM260480 | 753,133 | 0 | T | C | 100.0%
| 103.1
/ NA
| 27 | K37E (AAG→GAG) | h16_B0675 | Acyl‑CoA dehydrogenase,short‑chain specific |
| Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (13/14); total (13/14) |
CCAGTTCGCGCTGCACCACCAGCTGGCTGGCGGGGCCTTCGCCGAAGCCGCCGAAGTCGGCCGGCACGGTCAGGCCCAGCACGCCCATTTCAGCGAGCTTGTTCCACACGCCGCGATCCAGGCTCTTGCCTGCGCGCGCGCTCTTGCGGCGGGCCTCGAACGTGTATTCGGTATCGATGAAGCGACGCAGGCTGTCCGCCAGCATCTGTTGCTCTTCGCTGTAGGTGAAGTCCATGTCAGCTATCCCTT > AM260480/753007‑753255
|
ccAGTTCGCGCTGCACCACCAGCTGGCTGGCGGGGCCTTCGCCGAAGCCGCCGAAGTCGGCCGGCACGGTCAGGCCCAGCACGCCCATTTCAGCGAGCTTGTTCCACACGCCGCGATCCAGGCTCTCGCCTgcg > 2:351009/1‑134 (MQ=255)
caccacCAGCTGGCTGGCGGGGCCTTCGCCGAAGCCGCCGAAGTCGGCCGGCACGGTCAGGCCCAGCACGCCCATTTCAGCGAGCTTGTTCCACACGCCGCGATCCAGGCTCTCGCCTGCGCGCGCGCTCTTgc < 1:351009/134‑1 (MQ=255)
aGCTGGCTGGCGGGGCCTTCGCCGAAGCCGCCGAAGTCGGCCGGCACGGTCAGGCCCAGCACGCCCATTTCAGCGAGCTTGTTCCACACGCCGCGATCCAGGCTCTCGCCTGCGCGCGCGCTCTTGCGGCGGGc < 1:955743/134‑1 (MQ=255)
tggcGGGGCCTTCGCCGAAGCCGCCGAAGTCGGCCGGCACGGTCAGGCCCAGCACGCCCATTTCAGCGAGCTTGTTCCACACGCCGCGATCCAGGCTCTCGCCTGCGCGCGCGCTCTTGCGGCGGGCCTCGAAc > 1:358811/1‑134 (MQ=255)
cGGGGCCTTCGCCGAAGCCGCCGAAGTCGGCCGGCACGGTCAGGCCCAGCACGCCCATTTCAGCGAGCTTGTTCCACACGCCGCGATCCAGGCTCTCGCCTGCGCGCGCGCTCTTGCGGCGGGCCTCGAACgtg < 1:167465/134‑1 (MQ=255)
ggCCTTCGCCGAAGCCGCCGAAGTCGGCCGGCACGGTCAGGCCCAGCACGCCCATTTCAGCGAGCTTGTTCCACACGCCGCGATCCAGGCTCTCGCCTGCGCGCGCGCTCTTGcggcgg < 1:28355/119‑1 (MQ=255)
ggCCTTCGCCGAAGCCGCCGAAGTCGGCCGGCACGGTCAGGCCCAGCACGCCCATTTCAGCGAGCTTGTTCCACACGCCGCGATCCAGGCTCTCGCCTGCGCGCGCGCTCTTGcggcgg > 2:28355/1‑119 (MQ=255)
gAAGCCGCCGAAGTCGGCCGGCACGGTCAGGCCCAGCACGCCCATTTCAGCGAGCTTGTTCCACACGCCGCGATCCAGGCTCTCGCCTgcg > 2:304673/1‑91 (MQ=255)
gAAGCCGCCGAAGTCGGCCGGCACGGTCAGGCCCAGCACGCCCATTTCAGCGAGCTTGTTCCACACGCCGCGATCCAGGCTCTCGCCTgcg < 1:304673/91‑1 (MQ=255)
gAAGCCGCCGAAGTCGGCCGGCACGGTCAGGCCCAGCACGCCCATTTCAGCGAGCTTGTTCCACACGCCGCGATCCAGGCTCTCGCCTGCGCGCGCGCTCTTGCGGCGGGCCTCGAACGTGTATTCGGTATCGa < 2:358811/134‑1 (MQ=255)
cgccgAAGTCGGCCGGCACGGTCAGGCCCAGCACGCCCATTTCAGCGAGCTTGTTCCACACGCCGCGATCCAGGCTCTCGCCTGCGCGCGCGCTCTTGCGGCGGGCCTCGAACGTGTATTCGGTATCGATGaa > 1:431637/1‑133 (MQ=255)
cgccgAAGTCGGCCGGCACGGTCAGGCCCAGCACGCCCATTTCAGCGAGCTTGTTCCACACGCCGCGATCCAGGCTCTCGCCTGCGCGCGCGCTCTTGCGGCGGGCCTCGAACGTGTATTCGGTATCGATGaa < 2:431637/133‑1 (MQ=255)
cGCCCATTTCAGCGAGCTTGTTCCACACGCCGCGATCCAGGCTCTCGCCTgcgcg < 1:682869/55‑1 (MQ=255)
cGCCCATTTCAGCGAGCTTGTTCCACACGCCGCGATCCAGGCTCTCGCCTgcgcg > 2:682869/1‑55 (MQ=255)
cccATTTCAGCGAGCTTGTTCCACACGCCGCGATCCAGGCTCTCGCCTGCGCGCGCGCTCtt > 2:379317/1‑62 (MQ=255)
cccATTTCAGCGAGCTTGTTCCACACGCCGCGATCCAGGCTCTCGCCTGCGCGCGCGCTCtt < 1:379317/62‑1 (MQ=255)
cccATTTCAGCGAGCTTGTTCCACACGCCGCGATCCAGGCTCTCGCCTGCGCGCGCGCTCTTg > 2:808523/1‑63 (MQ=255)
cccATTTCAGCGAGCTTGTTCCACACGCCGCGATCCAGGCTCTCGCCTGCGCGCGCGCTCTTg < 1:808523/63‑1 (MQ=255)
tttCAGCGAGCTTGTTCCACACGCCGCGATCCAGGCTCTCGCCTGCGCGCGCGCTCTTGCGGCGGGCCTCGAACGTGTATTCGGTATCGATGAAGCGACGCAGGCTGTCCGCCAGCATCTGTTGCTCTTCGCTg > 2:508941/1‑134 (MQ=255)
cTTGTTCCACACGCCGCGATCCAGGCTCTCGCCTGCGCGCGCGCTCTTGCGGCGGGCCTCGAACGTGTATTCGGTATCGATGAAGCGACGCAGGCTGTCCGCCAGCATCTGTTGCTCTTCGCTGTAGGTGAAGt < 1:508941/134‑1 (MQ=255)
ttCCACACGCCGCGATCCAGGCTCTCGCCTGCGCGCGCGCTCTTGCGGCGGGCCTCGAACGTGTATTCGGTATCGa < 1:66429/76‑1 (MQ=255)
ttCCACACGCCGCGATCCAGGCTCTCGCCTGCGCGCGCGCTCTTGCGGCGGGCCTCGAACGTGTATTCGGTATCGa > 2:66429/1‑76 (MQ=255)
aTCCAGGCTCTCGCCTGCGCGCGCGCTCTTGCGGCGGGCCTCGAACgtg > 1:63650/1‑49 (MQ=255)
aTCCAGGCTCTCGCCTGCGCGCGCGCTCTTGCGGCGGGCCTCGAACgtg < 2:63650/49‑1 (MQ=255)
aTCCAGGCTCTCGCCTGCGCGCGCGCTCTTGCGGCGGGCCTCGAACGTGTATTCGGTATCGATGAAGCGACGCAGGCTGTCCGCCAGCATCTGTTGCTCTTCGCTGTAGGTGAAGTCCATGTCAGc < 2:332484/126‑1 (MQ=255)
aTCCAGGCTCTCGCCTGCGCGCGCGCTCTTGCGGCGGGCCTCGAACGTGTATTCGGTATCGATGAAGCGACGCAGGCTGTCCGCCAGCATCTGTTGCTCTTCGCTGTAGGTGAAGTCCATGTCAGc > 1:332484/1‑126 (MQ=255)
aTCCAGGCTCTCGCCTGCGCGCGCGCTCTTGCGGCGGGCCTCGAACGTGTATTCGGTATCGATGAAGCGACGCAGGCTGTCCGCCAGCATCTGTTGCTCTTCGCTGTAGGTGAAGTCCATGTCAGCTATCCCtt > 1:377888/1‑134 (MQ=255)
|
CCAGTTCGCGCTGCACCACCAGCTGGCTGGCGGGGCCTTCGCCGAAGCCGCCGAAGTCGGCCGGCACGGTCAGGCCCAGCACGCCCATTTCAGCGAGCTTGTTCCACACGCCGCGATCCAGGCTCTTGCCTGCGCGCGCGCTCTTGCGGCGGGCCTCGAACGTGTATTCGGTATCGATGAAGCGACGCAGGCTGTCCGCCAGCATCTGTTGCTCTTCGCTGTAGGTGAAGTCCATGTCAGCTATCCCTT > AM260480/753007‑753255
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
BRESEQ :: bam2aln output
CCCAGTTCGCGCTGCACCACCAGCTGGCTGGCGGGGCCTTCGCCGAAGCCGCCGAAGTCGGCCGGCACGGTCAGGCCCAGCACGCCCATTTCAGCGAGCTTGTTCCACACGCCGCGATCCAGGCTCTTGCCTGCGCGCGCGCTCTTGCGGCGGGCCTCGAACGTGTATTCGGTATCGATGAAGCGACGCAGGCTGTCCGCCAGCATCTGTTGCTCTTCGCTGTAGGTGAAGTCCATGTCAGCTATCCCTTCAGAAACCCAGGATCATCTTGG > AM260480/753006‑753277
|
gtgggctcggagatgtgtataagagacagGGCGGGGCCTTCGCCGAAGCCGCCGAAGTCGGCCGGCACGGTCAGGCCCAGCACGCCCATTTCAGCGAGCTTGTTCCACACGCCGCGATCCAGGCTCTCGCCTGCGCGCGCGCTCTTGCGG < GWNJ‑0478:712:GW2002102894th:2:1205:15171:44813/121‑1 (MQ=60)
aagcagaagacggcatacgagatgtagctccgtctcgtgggctcggagatgtgtataagagacagCGGTCAGGCCCAGCACGCCCATTTCAGCGAGCTTGTTCCACACGCCGCGATCCAGGCTCTCGCCTGCGCGCGCGCTCTTGCGGCG < GWNJ‑0478:712:GW2002102894th:2:2110:17465:81278/85‑1 (MQ=60)
agacggcatacgagatgtagctccgtctcgtgggctcggagatgtgtataagagacaGGTCAGGCCCAGCACGCCCATTTCAGCGAGCTTGTTCCACACGCCGCGATCCAGGCTCTCGCCTGCGCGCGCGCTCTTGCGGCGGGCCTCGAA < GWNJ‑0478:712:GW2002102894th:2:1209:13932:21027/93‑1 (MQ=60)
gacggcatacgagatgtagctccgtctcgtgggctcggagatgtgtataagagacaGGTCAGGCCCAGCACGCCCATTTCAGCGAGCTTGTTCCACACGCCGCGATCCAGGCTCTCGCCTGCGCGCGCGCTCTTGCGGCGGGCCTCGAAC < GWNJ‑0478:712:GW2002102894th:2:2202:10817:46543/94‑1 (MQ=60)
GCACCACCAGCTGGCTGGCGGGGCCTTCGCCGAAGCCGCCGAAGTCGGCCGGCACGGTCAGGCCCAGCACGCCCATTTCAGCGAGCTTGTTCCACACGCCGCGATCCAGGCTCTCGCCTGCGCGCGCGCTCTTGCGGCGGGCCTCGAACG > GWNJ‑0478:712:GW2002102894th:2:1206:13314:93918/1‑150 (MQ=60)
GCACCACCAGCTGGCTGGCGGGGCCTTCGCCGAAGCCGCCGAAGTCGGCCGGCACGGTCAGGCCCAGCACGCCCATTTCAGCGAGCTTGTTCCACACGCCGCGATCCAGGCTCTCGCCTGCGCGCGCGCTCTTGCGGCGGGCCTCGAACG < GWNJ‑0478:712:GW2002102894th:2:1206:6067:44953/150‑1 (MQ=60)
GCCAGCTGGCTGGCGGGGCCTTCGCCGAAGCCGCCGAAGTCGGCCGGCACGGTCAGGCCCAGCACGCCCATTTCAGCGAGCTTGTTCCACACGCCGCGATCCAGGCTCTCGCCTGCGCGCGCGCTCTTGCGGCGGGCCTCGAACGTGTAT < GWNJ‑0478:712:GW2002102894th:2:1103:11187:99547/150‑1 (MQ=60)
CAGCTGGCTGGCGGGGCCTTCGCCGAAGCCGCCGAAGTCGGCCGGCACGGTCAGGCCCAGCACGCCCATTTCAGCGAGCTTGTTCCACACGCCGCGATCCAGGCTCTCGCCTGCGCGCGCGCTCTTGCGGCGGGCCTCGAACGTGTATTC < GWNJ‑0478:712:GW2002102894th:2:2212:18952:64819/150‑1 (MQ=60)
cctcgttgtattttttttttttcaagcagaagacggcatacgagatgtagctccgtctcgtgggctcggagatgtgtataagagacaGATCCAGGCTCTCGCCTGCGCGCGCGCTCTTGCGGCGGGCCTCGAACGTGTATTCGGTAT < GWNJ‑0478:712:GW2002102894th:2:2201:11126:8634/60‑1 (MQ=60)
GCGGGGCCTTCGCCGAAGCCGCCGAAGTCGGCCGGCACGGTCAGGCCCAGCACGCCCATTTCAGCGAGCTTGTTCCACACGCCGCGATCCAGGCTCTCGCCTGCGCGCGCGCTCTTGCGGCGGGCCTCGAACGTGTATTCGGTATCGATG < GWNJ‑0478:712:GW2002102894th:2:1110:10715:89265/150‑1 (MQ=60)
GGCCTTCGCCGAAGCCGCCGAAGTCGGCCGGCACGGTCAGGCCCAGCACGCCCATTTCAGCGAGCTTGTTCCACACGCCGCGATCCAGGCTCTCGCCTGCGCGCGCGCTCTTGCGGCGGGCCTCGAACGTGTATTCGGTATCGATGAAGC > GWNJ‑0478:712:GW2002102894th:2:1213:13343:23977/1‑150 (MQ=60)
atgtagctccgtctcgtgggctcggagatgtgtataagagacagATTTCAGCGAGCTTGTTCCACACGCCGCGATCCAGGCTCTCGCCTGCGCGCGCGCTCTTGCGGCGGGCCTCGAACGTGTATTCGGTATCGATGAAGCGACGCAGGC < GWNJ‑0478:712:GW2002102894th:2:1104:14631:59857/106‑1 (MQ=60)
cgtgggctcggagatgtgtataagagaCAGGCTCTCGCCTGCGCGCGCGCTCTTGCGGCGGGCCTCGAACGTGTATTCGGTATCGATGAAGCGACGCAGGCTGTCCGCCAGCATCTGTTGCTCTTCGCTGTAGGTGAAGTCCATGTCAGC < GWNJ‑0478:712:GW2002102894th:2:2116:10820:15645/123‑1 (MQ=60)
GCTTGTTCCACACGCCGCGATCCAGGCTCTCGCCTGCGCGCGCGCTCTTGCGGCGGGCCTCGAACGTGTATTCGGTATCGATGAAGCGACGCAGGCTGTCCGCCAGCATCTGTTGCTCTTCGCTGTAGGTGAAGTCCATGTCAGCTATCC < GWNJ‑0478:712:GW2002102894th:2:1216:4356:42312/150‑1 (MQ=60)
GTTCCACACGCCGCGATCCAGGCTCTCGCCTGCGCGCGCGCTCTTGCGGCGGGCCTCGAACGTGTATTCGGTATCGATGctgtctcttatacacatctccgagcccacgagacggagctacatctcgtatgccgtcttctgcttgaaaa > GWNJ‑0478:712:GW2002102894th:2:1104:11856:40543/1‑79 (MQ=60)
GTTCCACACGCCGCGATCCAGGCTCTCGCCTGCGCGCGCGCTCTTGCGGCGGGCCTCGAACGTGTATTCGGTATCGATGAAGCGACGCAGGCTGTCCGCCAGCATCTGTTGCTCTTCGCTGTAGGTGAAGTCCATGTCAGCTATCCCTTC > GWNJ‑0478:712:GW2002102894th:2:1209:18587:11309/1‑150 (MQ=60)
GTTCCACACGCCGCGATCCAGGCTCTCGCCTGCGCGCGCGCTCTTGCGGCGGGCCTCGAACGTGTATTCGGTATCGATGAAGCGACGCAGGCTGTCCGCCAGCATCTGTTGCTCTTCGCTGTAGGTGAAGTCCATGTCAGCTATCCCTTC > GWNJ‑0478:712:GW2002102894th:2:1207:17703:30235/1‑150 (MQ=60)
ATCCAGGCTCTCGCCTGCGCGCGCGCTCTTGCGGCGGGCCTCGAACGTGTATTCGGTATCGATGAAGCGACGCAGGCTGTCCGCCAGCATCTGTTGCTCTTCGCTGTAGGTGAAGTCCATGTCAGCTATCCCTTCAGAAACCCAGGATC > GWNJ‑0478:712:GW2002102894th:2:1214:12447:18972/1‑149 (MQ=60)
GCTCTCGCCTGCGCGCGCGCTCTTGCGGCGGGCCTCGAACGTGTATTCGGTATCGATGAAGCGACGCAGGCTGTCCGCCAGCATCTGTTGCTCTTCGCTGTAGGTGAAGTCCATGTCAGCTATCCCTTCAGAAACCCAGGATCATCTTGG > GWNJ‑0478:712:GW2002102894th:2:2101:17881:86496/1‑150 (MQ=60)
|
CCCAGTTCGCGCTGCACCACCAGCTGGCTGGCGGGGCCTTCGCCGAAGCCGCCGAAGTCGGCCGGCACGGTCAGGCCCAGCACGCCCATTTCAGCGAGCTTGTTCCACACGCCGCGATCCAGGCTCTTGCCTGCGCGCGCGCTCTTGCGGCGGGCCTCGAACGTGTATTCGGTATCGATGAAGCGACGCAGGCTGTCCGCCAGCATCTGTTGCTCTTCGCTGTAGGTGAAGTCCATGTCAGCTATCCCTTCAGAAACCCAGGATCATCTTGG > AM260480/753006‑753277
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
