Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F1 I1 R1
|
154 |
26.6 |
2069536 |
90.2% |
1866721 |
108.9 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
AM260480 |
1,326,746 |
+G |
intergenic (+47/‑123) |
h16_B1172 → / → h16_B1173 |
Amidase/MFS transporter |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | AM260480 | 1,326,745 | 1 | . | G | 100.0%
| 95.5
/ NA
| 26 | intergenic (+46/‑124) | h16_B1172/h16_B1173 | Amidase/MFS transporter |
| Reads supporting (aligned to +/- strand): ref base . (0/0); new base G (11/15); total (11/15) |
ATACGGCTGGCCTATGCGGCCGAGCAGGCCTTGCGCGCGCGCCGGCCGCCGCATTCGACGCCGGCGCTGGAATAGGCGCGCGCCGGAACGACGTTTTATCGCTGCTCTACAAACGCCCGCC‑GACAC‑GCGGCGACAA‑GTCGCGGACATAGCGCGACAACCTCGAAACGAGGAGGAGACAAAGATGGGCGCAGAAACAGTAGTTCAATCGCCGAATTCCCCACAGCAGCATGAGCT > AM260480/1326625‑1326858
|
aTACGGCTGGCCTATGCGGCCGAGCAGGCCTTGCGCGCGCGCCGGCCGCCGCATTCGACGCCGGCGCTGGAATAGGCGCGCGCCGGAACGACGTTTTATCGCTGCTCTACAAACGCCCGCCGGACAc‑ggcggc < 2:989268/133‑6 (MQ=255)
ggCTGGCCTATGCGGCCGAGCAGGCCTTGCGCGCGCGCCGGCCGCCGCATTCGACGCCGGCGCTGGAATAGGCGCGCGCCGGAACGACGTTTTATCGCTGCTCTACAAACGCCCGCCGGACAc‑ggcggc > 1:373915/1‑124 (MQ=255)
ggCTGGCCTATGCGGCCGAGCAGGCCTTGCGCGCGCGCCGGCCGCCGCATTCGACGCCGGCGCTGGAATAGGCGCGCGCCGGAACGACGTTTTATCGCTGCTCTACAAACGCCCGCCGGACAc‑ggcggc < 2:373915/129‑6 (MQ=255)
ccTATGCGGCCGAGCAGGCCTTGCGCGCGCGCCGGCCGCCGCATTCGACGCCGGCGCTGGAATAGGCGCGCGCCGGAACGACGTTTTATCGCTGCTCTACAAACGCCCGCCGGACACGGCGGCGACAA‑ggtcgc > 2:691145/1‑129 (MQ=255)
tGCGGCCGAGCAGGCCTTGCGCGCGCGCCGGCCGCCGCATTCGACGCCGGCGCTGGAATAGGCGCGCGCCGGAACGACGTTTTATCGCTGCTCTACAAACGCCCGCCGGACACGGCGGCGACAAGGTCGCGGa > 1:871185/1‑133 (MQ=255)
cGAGCAGGCCTTGCGCGCGCGCCGGCCGCCGCATTCGACGCCGGCGCTGGAATAGGCGCGCGCCGGAACGACGTTTTATCGCTGCTCTACAAACGCCCGCCGGACAc‑ggcgg > 1:208869/1‑108 (MQ=255)
cGAGCAGGCCTTGCGCGCGCGCCGGCCGCCGCATTCGACGCCGGCGCTGGAATAGGCGCGCGCCGGAACGACGTTTTATCGCTGCTCTACAAACGCCCGCCGGACAc‑ggcgg < 2:208869/112‑5 (MQ=255)
gCAGGCCTTGCGCGCGCGCCGGCCGCCGCATTCGACGCCGGCGCTGGAATAGGCGCGCGCCGGAACGACGTTTTATCGCTGCTCTACAAACGCCCGCCGGACACGGCGGCGACAAGGTCGCGGACATAGCGCGa > 2:1033381/1‑134 (MQ=255)
gCAGGCCTTGCGCGCGCGCCGGCCGCCGCATTCGACGCCGGCGCTGGAATAGGCGCGCGCCGGAACGACGTTTTATCGCTGCTCTACAAACGCCCGCCGGACACGGCGGCGACAAGGTCGCGGACATAGCGCGa > 2:574543/1‑134 (MQ=255)
gccgcATTCGACGCCGGCGCTGGAATAGGCGCGCGCCGGAACGACGTTTTATCGCTGCTCTACAAACGCCCGCCGGACACGGCGGCGACAAGGTCGCGGACATAGCGCGACAACCTCGAAACGAGGAGGAGAc > 2:438678/1‑133 (MQ=255)
gccgcATTCGACGCCGGCGCTGGAATAGGCGCGCGCCGGAACGACGTTTTATCGCTGCTCTACAAACGCCCGCCGGACACGGCGGCGACAAGGTCGCGGACATAGCGCGACAACCTCGAAACGAGGAGGAGACa < 1:900051/134‑1 (MQ=255)
cgcATTCGACGCCGGCGCTGGAATAGGCGCGCGCCGGAACGACGTTTTATCGCTGCTCTACAAACGCCCGCCGGACACGGCGGCGACAAGGTCGCGGACATAGCGCGACAACCTCGAAACGAGGAGGAGACaaa < 1:438678/134‑1 (MQ=255)
cATTCGACGCCGGCGCTGGAATAGGCGCGCGCCGGAACGACGTTTTATCGCTGCTCTACAAACGCCCGCCGGACACGGCGGCGACAAGGTCGCGGACATAGCGCGACAACCTCGAAACGAGGAGGAGACAAAGa < 1:574543/134‑1 (MQ=255)
tCGACGCCGGCGCTGGAATAGGCGCGCGCCGGAACGACGTTTTATCGCTGCTCTACAAACGCCCGCCGGACACGGCGGCGACAAGGTCGCGGACATAGCGCGACAACCTCGAAACgaggagga < 1:922570/123‑1 (MQ=255)
tCGACGCCGGCGCTGGAATAGGCGCGCGCCGGAACGACGTTTTATCGCTGCTCTACAAACGCCCGCCGGACACGGCGGCGACAAGGTCGCGGACATAGCGCGACAACCTCGAAACgaggagga > 2:922570/1‑123 (MQ=255)
aCGCCGGCGCTGGAATAGGCGCGCGCCGGAACGACGTTTTATCGCTGCTCTACAAACGCCCGCCGGACACGGCGGCGACAAGGTCGCGGACATAGCGCGACAACCTCGAAACGAGGAGGAGACAAAGATGGgcg < 1:1033381/134‑1 (MQ=255)
cGCCGGCGCTGGAATAGGCGCGCGCCGGAACGACGTTTTATCGCTGCTCTACAAACGCCCGCCGGACACGGCGGCGACAAGGTCGCGGACATAGCGCGAc < 1:46055/100‑1 (MQ=255)
cGCCGGCGCTGGAATAGGCGCGCGCCGGAACGACGTTTTATCGCTGCTCTACAAACGCCCGCCGGACACGGCGGCGACAAGGTCGCGGACATAGCGCGAc > 2:46055/1‑100 (MQ=255)
cgcgcCGGAACGACGTTTTATCGCTGCTCTACAAACGCCCGCCGGACACGGCGGCGACAAGGTCGCGGACATAGCGCGACAACCTCGAAACGAGGAGGAGACAAAGATGGGCGCAGAAACAGTAGTTCAATCGc < 1:897674/134‑1 (MQ=255)
cGGAACGACGTTTTATCGCTGCTCTACAAACGCCCGCCGGACACGGCGGCGACAAGGTCGCGGACATAGCGCGACAACCTCGAAACGAGGAGGAGACAAAGAt < 1:726852/103‑1 (MQ=255)
cGGAACGACGTTTTATCGCTGCTCTACAAACGCCCGCCGGACACGGCGGCGACAAGGTCGCGGACATAGCGCGACAACCTCGAAACGAGGAGGAGACAAAGAt > 2:726852/1‑103 (MQ=255)
gacgTTTTATCGCTGCTCTACAAACGCCCGCCGGACACGGCGGCGACAAGGTCGCGGACATAGCGCGACAACCTCGAAACga > 2:790393/1‑82 (MQ=255)
gacgTTTTATCGCTGCTCTACAAACGCCCGCCGGACACGGCGGCGACAAGGTCGCGGACATAGCGCGACAACCTCGAAACga < 1:790393/82‑1 (MQ=255)
ctctACAAACGCCCGCCGGACACGGCGGCGACAAGGTCGCGGACATAGCGCGACAACCTCGAAACGAGGAGGAGACAAAGATGGGCGCAGAAACAGTAGTTCAATCGCCGAATTCCCCACAGCAGCATGAGCt < 1:691145/133‑1 (MQ=255)
ctctACAAACGCCCGCCGGACACGGCGGCGACAAGGTCGCGGACATAGCGCGACAACCTCGAAACGAGGAGGAGACAAAGATGGGCGCAGAAACAGTAGTTCAATCGCCGAATTCCCCACAGCAGCATGAGCt < 2:11650/133‑1 (MQ=255)
ctctACAAACGCCCGCCGGACACGGCGGCGACAAGGTCGCGGACATAGCGCGACAACCTCGAAACGAGGAGGAGACAAAGATGGGCGCAGAAACAGTAGTTCAATCGCCGAATTCCCCACAGCAGCATGAGCt < 2:907784/133‑1 (MQ=255)
cgcccgccgGACACGGCGGCGACAAGGTCGCGGACATAGCGCGACAACCTCGAAACGAGGAGGAGACAAAGATGGGCGCAGAAACAGTAGTTCAATCGCCGAATTCCCCACAGCAGCATGAGCt < 1:997136/124‑1 (MQ=255)
cgcccgccgGACACGGCGGCGACAAGGTCGCGGACATAGCGCGACAACCTCGAAACGAGGAGGAGACAAAGATGGGCGCAGAAACAGTAGTTCAATCGCCGAATTCCCCACAGCAGCATGAGCt > 2:997136/1‑124 (MQ=255)
|
ATACGGCTGGCCTATGCGGCCGAGCAGGCCTTGCGCGCGCGCCGGCCGCCGCATTCGACGCCGGCGCTGGAATAGGCGCGCGCCGGAACGACGTTTTATCGCTGCTCTACAAACGCCCGCC‑GACAC‑GCGGCGACAA‑GTCGCGGACATAGCGCGACAACCTCGAAACGAGGAGGAGACAAAGATGGGCGCAGAAACAGTAGTTCAATCGCCGAATTCCCCACAGCAGCATGAGCT > AM260480/1326625‑1326858
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
BRESEQ :: bam2aln output
CTTTCACCGAACCCGTGCTGATACGGCTGGCCTATGCGGCCGAGCAGGCCTTGCGCGCGCGCCGGCCGCCGCATTCGACGCCGGCGCTGGAATAGGCGCGCGCCGGAACGACGTTTTATCGCTGCTCTACAAACGCCCGCC‑GACAC‑GCGGCGACAA‑GTCGCGGACATAGCGCGACAACCTCGAAACGAGGAGGAGACAAAGATGGGCGCAGAAACAGTAGTTCAATCGCCGAATTCCCCACAGCAGCATGAGCTCGACCGGGCCATGGATGGC > AM260480/1326605‑1326877
|
cccggtccctatttttttttcaagcagaagacggcatacgagatgtagctccgtctcgtgggctcggagatgtgtataagagacaGCTGGAATAGGCGCGCGCCGGAACGACGTTTTATCGCTGCTCTACAAACGCCCGCCGGACAC < GWNJ‑0478:712:GW2002102894th:2:1216:17941:60674/62‑1 (MQ=60)
AACCCGTGCTGATACGGCTGGCCTATGCGGCCGAGCAGGCCTTGCGCGCGCGCCGGCCGCCGCATTCGACGCCGGCGCTGGAATAGGCGCGCGCCGGAACGACGTTTTATCGCTGCTCTACAAACGCCCGCCGGACACGGCGGCGACA > GWNJ‑0478:712:GW2002102894th:2:1208:13374:87078/1‑148 (MQ=60)
GATACGGCTGGCCTATGCGGCCGAGCAGGCCTTGCGCGCGCGCCGGCCGCCGCATTCGACGCCGGCGCTGGAATAGGCGCGCGCCGGAACGACGTTTTATCGCTGCTCTACAAACGCCCGCCGGACACGGCGGCGACAAGGTCGCGGAC > GWNJ‑0478:712:GW2002102894th:2:2206:11047:53201/1‑149 (MQ=60)
GATACGGCTGGCCTATGCGGCCGAGCAGGCCTTGCGCGCGCGCCGGCCGCCGCATTCGACGCCGGCGCTGGAATAGGCGCGCGCCGGAACGACGTTTTATCGCTGCTCTACAAACGCCCGCC‑Ggacctgtctcttatacacatctccg > GWNJ‑0478:712:GW2002102894th:2:2112:18043:76880/1‑123 (MQ=60)
GCTGGCCTATGCGGCCGAGCAGGCCTTGCGCGCGCGCCGGCCGCCGCATTCGACGCCGGCGCTGGAATAGGCGCGCGCCGGAACGACGTTTTATCGCTGCTCTACAAACGCCCGCCGGACACGGCGGCGACAAGGTCGCGGACtgtctct > GWNJ‑0478:712:GW2002102894th:2:1115:11419:29244/1‑143 (MQ=60)
gagatgtgtataagagacagGGCCGCCGCATTCGACGCCGGCGCTGGAATAGGCGCGCGCCGGAACGACGTTTTATCGCTGCTCTACAAACGCCCGCCGGACACGGCGGCGACAAGGTCGCGGACATAGCGCGACAACCTCGAAACGAGG < GWNJ‑0478:712:GW2002102894th:2:1102:7578:17339/130‑1 (MQ=60)
ccgtctcgtgggctcggagatgtgtataagagacagGGCGCGCGCCGGAACGACGTTTTATCGCTGCTCTACAAACGCCCGCCGGACACGGCGGCGACAAGGTCGCGGACATAGCGCGACAACCTCGAAACGAGGAGGAGACAAAGAT < GWNJ‑0478:712:GW2002102894th:2:2203:16739:22050/112‑1 (MQ=60)
CGGCCGCCGCATTCGACGCCGGCGCTGGAATAGGCGCGCGCCGGAACGACGTTTTATCGCTGCTCTACAAACGCCCGCCGGACACGGCGGCGACAAGGTCGCGGACATAGCGCGACAACCTCGAAACGAGGAGGAGACAAAGATGGGCGC < GWNJ‑0478:712:GW2002102894th:2:2211:12199:71618/150‑1 (MQ=60)
CGCCGCATTCGACGCCGGCGCTGGAATAGGCGCGCGCCGGAACGACGTTTTATCGCTGCTCTACAAACGCCCGCCGGACACGGCGGCGACAAGGTCGCGGACATAGCGCGACAACCTCGAAACGAGGAGGAGACAAAGATGGGCGCAGAA < GWNJ‑0478:712:GW2002102894th:2:2208:15279:34307/150‑1 (MQ=60)
CCGCATTCGACGCCGGCGCTGGAATAGGCGCGCGCCGGAACGACGTTTTATCGCTGCTCTACAAACGCCCGCCGGACACGGCGGCGACAAGGTCGCGGACATAGCGCGACAACCTCGAAACGAGGAGGAGACAAAGATGGGCGCAGAAAC < GWNJ‑0478:712:GW2002102894th:2:1213:7459:61823/150‑1 (MQ=60)
GCATTCGACGCCGGCGCTGGAATAGGCGCGCGCCGGAACGACGTTTTATCGCTGCTCTACAAACGCCCGCCGGACACGGCGGCGACAAGGTCGCGGACATAGCGCGACAACCTCGAAACGAGGAGGAGACAAAGATGGGCGCAGAAACAG < GWNJ‑0478:712:GW2002102894th:2:2105:9939:97892/150‑1 (MQ=60)
atgtgtataagagacagGGAATAGGCGCGCGCCGGAACGACGTTTTATCGCTGCTCTACAAACGCCCGCCGGACACGGCGGCGACAAGGTCGCGGACATAGCGCGACAACCTCGAAACGAGGAGGAGACAAAGATGGGCGCAGAAACAGT < GWNJ‑0478:712:GW2002102894th:2:2115:2220:38336/133‑1 (MQ=60)
GACGCCGGCGCTGGAATAGGCGCGCGCCGGAACGACGTTTTATCGCTGCTCTACAAACGCCCGCCGGACACGGCGGCGACAAGGTCGCGGACATAGCGCGACAACCTCGAAACGAGGAGGAGACAAAGATGGGCGCAGAAACAGTAGTTC < GWNJ‑0478:712:GW2002102894th:2:2216:16868:93383/150‑1 (MQ=60)
GCGCGCCGGAACGACGTTTTATCGCTGCTCTACAAACGCCCGCCGGACACGGCGGCGACAAGGTCGCGGACATAGCGCGACAACCTCGAAACGAGGAGGAGACAAAGATGGGCGCAGAAACAGTAGTTCAATCGCCGAATTCCCCACAGC < GWNJ‑0478:712:GW2002102894th:2:2208:14072:19079/150‑1 (MQ=60)
ataagagacagGCTCTACAAACGCCCGCCGGACACGGCGGCGACAAGGTCGCGGACATAGCGCGACAACCTCGAAACGAGGAGGAGACAAAGATGGGCGCAGAAACAGTAGTTCAATCGCCGAATTCCCCACAGCAGCATGAGCTCGAC < GWNJ‑0478:712:GW2002102894th:2:1111:6656:44080/138‑1 (MQ=60)
GCTCTACAAACGCCCGCCGGACACGGCGGCGACAAGGTCGCGGACATAGCGCGACAACCTCGAAACGAGGAGGAGACAAAGATGGGCGCAGAAACAGTAGTTCAATCGCCGAATTCCCCACAGCAGCATGAGCTCGACCGGGCCtgtctc > GWNJ‑0478:712:GW2002102894th:2:2114:4477:33455/1‑144 (MQ=60)
GCTCTACAAACGCCCGCCGGACACGGCGGCGACAAGGTCGCGGACATAGCGCGACAACCTCGAAACGAGGAGGAGACAAAGATGGGCGCAGAAACAGTAGTTCAATCGCCGAATTCCCCACAGCAGCATGAGCTCGACCGGGCCATGGA < GWNJ‑0478:712:GW2002102894th:2:2214:10380:95397/149‑1 (MQ=60)
GCTCTACAAACGCCCGCCGGACACGGCGGCGACAAGGTCGCGGACATAGCGCGACAACCTCGAAACGAGGAGGAGACAAAGATGGGCGCAGAAACAGTAGTTCAATCGCCGAATTCCCCACAGCAGCATGAGCTCGACCGGGCCATGGA < GWNJ‑0478:712:GW2002102894th:2:2113:4001:29791/149‑1 (MQ=60)
CTACAAACGCCCGCCGGACACGGCGGCGACAAGGTCGCGGACATAGCGCGACAACCTCGAAACGAGGAGGAGACAAAGATGGGCGCAGAAACAGTAGTTCAATCGCCGAATTCCCCACAGCAGCATGAGCTCGACCGGGCCATGGATGGC > GWNJ‑0478:712:GW2002102894th:2:2210:13636:51577/1‑150 (MQ=60)
|
CTTTCACCGAACCCGTGCTGATACGGCTGGCCTATGCGGCCGAGCAGGCCTTGCGCGCGCGCCGGCCGCCGCATTCGACGCCGGCGCTGGAATAGGCGCGCGCCGGAACGACGTTTTATCGCTGCTCTACAAACGCCCGCC‑GACAC‑GCGGCGACAA‑GTCGCGGACATAGCGCGACAACCTCGAAACGAGGAGGAGACAAAGATGGGCGCAGAAACAGTAGTTCAATCGCCGAATTCCCCACAGCAGCATGAGCTCGACCGGGCCATGGATGGC > AM260480/1326605‑1326877
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
