Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F1 I1 R1
|
154 |
26.6 |
2069536 |
90.2% |
1866721 |
108.9 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
AM260480 |
1,537,213 |
G→A |
G347D (GGC→GAC) |
h16_B1373 → |
Acyl‑CoA synthetase (AMP‑forming)/AMP‑acid ligase II |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | AM260480 | 1,537,213 | 0 | G | A | 100.0%
| 106.9
/ NA
| 28 | G347D (GGC→GAC) | h16_B1373 | Acyl‑CoA synthetase (AMP‑forming)/AMP‑acid ligase II |
| Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (14/14); total (14/14) |
CATGGCTACGGCATGACCGAATATGCGGGGTCGCTGTTCGTGACCCGCATGGACCGGCCGCGCACCGATTGCTCGGCGGGCGAGATCGTCGAAGGTGCAGAGCTGCGGGTAGTCGGGCCGGAGGGCAAGCCGGTGCCGCAGGGGCAACCCGGCGAGCTATGGATCAGGGGGCCCGGCGTGATGCGCGGCTACTACCGCGCACCGGACCTGAGCGCCGAGGCCCTGCGGCCCGACGGCTGGCT > AM260480/1537089‑1537330
|
cATGGCTACGGCATGACCGAATATGCGGGGTCGCTGTTCGTGACCCGCATGGACCGGCCGCGCACCGATTGCTCGGCGGGCGAGATCGTCGAAGGTGCAGAGCTGCGGGTAGTCGGGCCGGAGGACAAGCCGGt < 1:764497/134‑1 (MQ=255)
cATGACCGAATATGCGGGGTCGCTGTTCGTGACCCGCATGGACCGGCCGCGCACCGATTGCTCGGCGGGCGAGATCGTCGAAGGTGCAGAGCTGCGGGTAGTCGGGCCGGAGGACAAGCCGGTGCCGCAgggg > 2:604948/1‑133 (MQ=255)
atatGCGGGGTCGCTGTTCGTGACCCGCATGGACCGGCCGCGCACCGATTGCTCGGCGGGCGAG‑TCGTCGAAGGTGCAGAGCTGCGGGTAGTCGGGCCGGAGGACAAGCCGGTGCCGCAGGGGCAACCCGGCGa < 1:197314/134‑1 (MQ=255)
cGGGGTCGCTGTTCGTGACCCGCATGGACCGGCCGCGCACCGATTGCTCGGCGGGCGAGATCGTCGAAGGTGCAGAGCTGCGGGTAGTCGGGCCGGAGGACAAGCCGGTGCCGCAGGGGCAACCCGGCg > 2:418485/1‑129 (MQ=255)
cGGGGTCGCTGTTCGTGACCCGCATGGACCGGCCGCGCACCGATTGCTCGGCGGGCGAGATCGTCGAAGGTGCAGAGCTGCGGGTAGTCGGGCCGGAGGACAAGCCGGTGCCGCAGGGGCAACCCGGCg < 1:418485/129‑1 (MQ=255)
ggACCGGCCGCGCACCGATTGCTCGGCGGGCGAGATCGTCGAAGGTGCAGAGCTGCGGGTAGTCGGGCCGGAGGACAAg < 1:795951/79‑1 (MQ=255)
ggACCGGCCGCGCACCGATTGCTCGGCGGGCGAGATCGTCGAAGGTGCAGAGCTGCGGGTAGTCGGGCCGGAGGACAAg > 2:795951/1‑79 (MQ=255)
ggACCGGCCGCGCACCGATTGCTCGGCGGGCGAGATCGTCGAAGGTGCAGAGCTGCGGGTAGTCGGGCCGGAGGACAAGCCGGTGCCGCAGGGGCAACCCGGCGAGCTATGGATCAGGGGGCCCGGCGTGATgc > 2:86839/1‑134 (MQ=255)
ggACCGGCCGCGCACCGATTGCTCGGCGGGCGAGATCGTCGAAGGTGCAGAGCTGCGGGTAGTCGGGCCGGAGGACAAGCCGGTGCCGCAGGGGCAACCCGGCGAGCTATGGATCAGGGGGCCCGGCGTGATgc > 2:759529/1‑134 (MQ=255)
ccGGCCGCGCACCGATTGCTCGGCGGGCGAGATCGTCGAAGGTGCAGAGCTGCGGGTAGTCGGGCCGGAGGACAAGCCGGTGCCGCAGGGGCAACCCGGCGAGCTATGGATCAGGGGGCCCGGCGTGATGcgcg < 2:373565/134‑1 (MQ=255)
cgcACCGATTGCTCGGCGGGCGAGATCGTCGAAGGTGCAGAGCTGCGGGTAGTCGGGCCGGAGGACAAGCCGGTGCCGCAGGGGCAACCCGGCGAGCTATGGATCAGGGGGCCCGGCGTg > 2:989842/1‑120 (MQ=255)
cgcACCGATTGCTCGGCGGGCGAGATCGTCGAAGGTGCAGAGCTGCGGGTAGTCGGGCCGGAGGACAAGCCGGTGCCGCAGGGGCAACCCGGCGAGCTATGGATCAGGGGGCCCGGCGTg < 1:989842/120‑1 (MQ=255)
gcATCGATTGCTCGGCGGGCGAGATCGTCGAAGGTGCAGAGCTGCGGGTAGTCGGGCCGGAGGACAAGCCGGTGCCGCAGGGGCAACCCGGCGAGCTATGGATCAGGGGGCCCGGCGTGATGCGCGGCtactac > 2:511276/1‑134 (MQ=255)
gcACCGATTGCTCGGCGGGCGAGATCGTCGAAGGTGCAGAGCTGCGGGTAGTCGGGCCGGAGGACAAGCCGGTGCCGCAGGGGCAACCCGGCGAGCTATGGATCAGGGGGCCCGGCGTGATGCGCGGCtactac > 2:325982/1‑134 (MQ=255)
ccGATTGCTCGGCGGGCGAGATCGTCGAAGGTGCAGAGCTGCGGGTAGTCGGGCCGGAGGACAAGCCGGTGCCGCAGGGGCAACCCGGCGAGCTATGGATCAGGGGGCCCGGCGTGATGCGCGGCTACTACcgc < 1:759529/134‑1 (MQ=255)
aGGTGCAGAGCTGCGGGTAGTCGGGCCGGAGGACAAGCCGGTGCCGCAGGGGCAACCCGGCGAGCTATGGATCAGGGGGCCCGGCGTGATGCGCGGCTACTACCGCGCACCGGACCTGAGCGCCGAGGCCCTGc < 1:511276/134‑1 (MQ=255)
aGGTGCAGAGCTGCGGGTAGTCGGGCCGGAGGACAAGCCGGTGCCGCAGGGGCAACCCGGCGAGCTATGGATCAGGGGGCCCGGCGTGATGCGCGGCTACTACCGCGCACCGGACCTGAGCGCCGAGGCCCTGc > 1:40134/1‑134 (MQ=255)
gagCTGCGGGTAGTCGGGCCGGAGGACAAGCCGGTGCCGCAGGGGCAACCCGGCGAGCTATGGATCAGGGGGCCCGGCGTGATGCGCGGCTACTACCGCGCACCGGACCTGAGCGCCGAGGCCCTGCGGCCCGa < 2:1025692/134‑1 (MQ=255)
gagCTGCGGGTAGTCGGGCCGGAGGACAAGCCGGTGCCGCAGGGGCAACCCGGCGAGCTATGGATCAGGGGGCCCGGCGTGATGCGCGGCTACTACCGCGCACCGGACCTGAGCGCCGAGGCCCTGCGGCCCGa < 1:604948/134‑1 (MQ=255)
gCTGCGGGTAGTCGGGCCGGAGGACAAGCCGGTGCCGCAgg > 1:884680/1‑41 (MQ=255)
gCTGCGGGTAGTCGGGCCGGAGGACAAGCCGGTGCCGCAgg < 2:884680/41‑1 (MQ=255)
tAGTCGGGCCGGAGGACAAGCCGGTGCCGCAGGGGCAACCCGGCGAGCTATGGATCAGGGGGCCCGGCGTGATgcgc < 1:994641/77‑1 (MQ=255)
tAGTCGGGCCGGAGGACAAGCCGGTGCCGCAGGGGCAACCCGGCGAGCTATGGATCAGGGGGCCCGGCGTGATgcgc > 2:994641/1‑77 (MQ=255)
tAGTCGGGCCGGAGGACAAGCCGGTGCCGCAGGGGCAACCCGGCGAGCTATGGATCAGGGGGCCCGGCGTGATGCGCGGCTACTACCGCGCACCGGACCTGAGCGCCGAGGCCCTGCGGCCCGACggctggct > 1:753077/1‑133 (MQ=255)
gCCGGAGGACAAGCCGGTGCCGCAGGGGCAACCCGGCGAGCTATGGATCAGGGGGCCCGGCGTGATGCGCGGCTACTACCGCGCACCGGACCTGAgc < 2:658344/97‑1 (MQ=255)
gCCGGAGGACAAGCCGGTGCCGCAGGGGCAACCCGGCGAGCTATGGATCAGGGGGCCCGGCGTGATGCGCGGCTACTACCGCGCACCGGACCTGAgc > 1:658344/1‑97 (MQ=255)
gCCGGAGGACAAGCCGGTGCCGCAGGGGCAACCCGGCGAGCTATGGATCAGGGGGCCCGGCGTGATGCGCGGCTACTACCGCGCACCGGACCTGAGCGCCGAGGCCCTGCGGCCCGa > 1:97482/1‑117 (MQ=255)
gCCGGAGGACAAGCCGGTGCCGCAGGGGCAACCCGGCGAGCTATGGATCAGGGGGCCCGGCGTGATGCGCGGCTACTACCGCGCACCGGACCTGAGCGCCGAGGCCCTGCGGCCCGa < 2:97482/117‑1 (MQ=255)
|
CATGGCTACGGCATGACCGAATATGCGGGGTCGCTGTTCGTGACCCGCATGGACCGGCCGCGCACCGATTGCTCGGCGGGCGAGATCGTCGAAGGTGCAGAGCTGCGGGTAGTCGGGCCGGAGGGCAAGCCGGTGCCGCAGGGGCAACCCGGCGAGCTATGGATCAGGGGGCCCGGCGTGATGCGCGGCTACTACCGCGCACCGGACCTGAGCGCCGAGGCCCTGCGGCCCGACGGCTGGCT > AM260480/1537089‑1537330
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
BRESEQ :: bam2aln output
GCGATGTTCGGCCAGCCGCTGCACCATGGCTACGGCATGACCGAATATGCGGGGTCGCTGTTCGTGACCCGCATGGACCGGCCGCGCACCGATTGCTCGGCGGGCGAGATCGTCGAAGGTGCAGAGCTGCGGGTAGTCGGGCCGGAGGGCAAGCCGGTGCCGCAGGGGCAACCCGGCGAGCTATGGATCAGGGGGCCCGGCGTGATGCGCGGCTACTACCGCGCACCGGACCTGAGCGCCGAGGCCCTGCGGCCCGACGGCTGGCTCAATACCGGCGACATCGGCCGGCTG > AM260480/1537065‑1537355
|
aacgcggtactcgatatttattttgtttttgtggtttttttttttcaagcagaagacggcatacgagatgtagctccgtctcgtgggctcgggatgtgtataagagacagCGTCGAAGGTGCAGAGCTGCGGGTAGTCGGGCCGGAGGAC < GWNJ‑0478:712:GW2002102894th:2:1105:16871:100005/40‑1 (MQ=60)
agctccgtctcgtgggctcggagatgtgtataagagacagGTTCGTGACCCGCATGGACCGGCCGCGCACCGATTGCTCGGCGGGCGAGATCGTCGAAGGTGCAGAGCTGCGGGTAGTCGGGCCGGAGGACAAGCCGGTGCCGCAGGGG < GWNJ‑0478:712:GW2002102894th:2:2203:11191:60037/109‑1 (MQ=60)
CCATGGCTACGGCATGACCGAATATGCGGGGTCGCTGTTCGTGACCCGCATGGACCGGCCGCGCACCGATTGCTCGGCGGGCGAGATCGTCGAAGGTGCAGAGCTGCGGGTAGTCGGGCCGGAGGACAAGCCGGTGCCGCAGGGGCAACC < GWNJ‑0478:712:GW2002102894th:2:2116:2905:50037/150‑1 (MQ=60)
GAATATGCGGGGTCGCTGTTCGTGACCCGCATGGACCGGCCGCGCACCGATTGCTCGGCGGGCGAGATCGTCGAAGGTGCAGAGCTGCGGGTAGTCGGGCCGGAGGACAAGCCGGTGCCtgtctcttatacacatctccgagcccacg > GWNJ‑0478:712:GW2002102894th:2:2203:10540:8408/1‑119 (MQ=60)
AATATGCGGGGTCGCTGTTCGTGACCCGCATGGACCGGCCGCGCACCGATTGCTCGGCGGGCGAGATCGTCGAAGGTGCAGAGCTGCGGGTAGTCGGGCCGGAGGACAAGCCGGTGCCGCAGGGGCAACCCGGCGAGCTATGGATCAGGG < GWNJ‑0478:712:GW2002102894th:2:1210:19027:47378/150‑1 (MQ=60)
AATATGCGGGGTCGCTGTTCGTGACCCGCATGGACCGGCCGCGCACCGATTGCTCGGCGGGCGAG‑TCGTCGAAGGTGCAGAGCTGCGGGTAGTCGGGCCGGAGGACAAGCCGGTGCCGCAGGGGCAACCCGGCGAGCTATGGATCAGGGG < GWNJ‑0478:712:GW2002102894th:2:1112:15058:62861/150‑1 (MQ=60)
ggagatgtgtataagagacaGGACCGGCCGCGCACCGATTGCTCGGCGGGCGAGATCGTCGAAGGTGCAGAGCTGCGGGTAGTCGGGCCGCAGGGCctcggcgctcaggtccggtgcgcggtagtagccgcgcatcacgccgggccctgc < GWNJ‑0478:712:GW2002102894th:2:2104:6111:40147/130‑55 (MQ=60)
CGCATGGACCGGCCGCGCACCGATTGCTCGGCGGGCGAGATCGTCGAAGGTGCAGAGCTGCGGGTAGTCGGGCCGGAGGACAAGCCctgtctcttatacacatctccgagcccacgagacggagctacatctcgtatgccgtcttctgct > GWNJ‑0478:712:GW2002102894th:2:2212:6773:28279/1‑86 (MQ=60)
CGCATGGACCGGCCGCGCACCGATTGCTCGGCGGGCGAGATCGTCGAAGGTGCAGAGCTGCGGGTAGTCGGGCCGGAGGACAAGCCGGTGCCGCAGGGGCAACCCGGCGAGCTATGGATCAGGGGGCCCGGCGTGATGCGCGGCTACTAC < GWNJ‑0478:712:GW2002102894th:2:2214:17445:54356/150‑1 (MQ=60)
gtagctccgtctcgtgggctcggagatgtgtataagagacAGGTGCAGAGCTGCGGGTAGTCGGGCCGGAGGACAAGCCGGTGCCGCAGGGGCAACCCGGCGAGCTATGGATCAGGGGGCCCGGCGTGATGCGCGGCTACTACCGCGCA < GWNJ‑0478:712:GW2002102894th:2:2214:15123:81978/109‑1 (MQ=60)
ACCGATTGCTCGGCGGGCGAGATCGTCGAAGGTGCAGAGCTGCGGGTAGTCGGGCCGGAGGACAAGCCGGTGCCGCAGGGGCAACCCGGCGAGCTATGGATCAGGGGGCCCGGCGTGATGCGCGGCTACTACCGCGCACCGGACCTGAGC < GWNJ‑0478:712:GW2002102894th:2:2116:7613:22022/150‑1 (MQ=60)
GGGCGAGATCGTCGAAGGTGCAGAGCTGCGGGTAGTCGGGCCGGAGGACAAGCCGGTGCCGCAGGGGCAACCCGGCGAGCTATGGATCAGGGGGCCCGGCGTGATGCGCGGCTACTACCGCGCACCGGACCTGAGCGCCGAGGCCCTGCG > GWNJ‑0478:712:GW2002102894th:2:1105:10112:77273/1‑150 (MQ=60)
CGTCGAAGGTGCAGAGCTGCGGGTAGTCGGGCCGGAGGACAAGCCGGTGCCGCAGGGGCAACCCGGCGAGCctgtctcttatacacatctccgagcccacgagacggagctacatctcgtatgccgtcttctgcttgaaaaaaaaaaaa > GWNJ‑0478:712:GW2002102894th:2:2207:11664:38269/1‑71 (MQ=60)
AAGGTGCAGAGCTGCGGGTAGTCGGGCCGGAGGACAAGCCGGTGCCGCAGGGGCAACCCGGCGAGCTATGGATCAGGGGGCCCGGCGTGATGCGCGGCTACTACCGCGCACCGGACCTGAGCGCCGAGGCCCTGCGGCaccggcttgtcc < GWNJ‑0478:712:GW2002102894th:2:1216:16865:54890/150‑13 (MQ=60)
GTGCAGAGCTGCGGGTAGTCGGGCCGGAGGACAAGCCGGTGCCGCAGGGGCAACCCGGCGAGCTATGGATCAGGGGGCCCGGCGTGATGCGCGGCTACTACCGCGCACCGGACCTGAGCGCCGAGGCCCTGCGGCCCGACGGCTGGCTC > GWNJ‑0478:712:GW2002102894th:2:2114:7670:83822/1‑149 (MQ=60)
AGAGCTGCGGGTAGTCGGGCCGGAGGACAAGCCGGTGCCGCAGGGGCAACCCGGCGAGCTATGGATCAGGGGGCCCGGCGTGATGCGCGGCTACTACCGCGCACCGGACCTGAGCGCCGAGGCCCTGCGGCCCGACGGCTGGCTCAATAC < GWNJ‑0478:712:GW2002102894th:2:2107:16577:98045/150‑1 (MQ=60)
GCTGCGGGTAGTCGGGCCGGAGGACAAGCCGGTGCCGCAGGGGCAACCCGGCGAGCTATGGATCAGGGGGCCCGGCGTGATGCGCGGCTACTACCGCGCACCGGACCTGAGCGCCGAGGCCCTGCGGCtgtctcttatacacatctccg > GWNJ‑0478:712:GW2002102894th:2:2111:6688:37475/1‑128 (MQ=60)
GCTGCGGGTAGTCGGGCCGGAGGACAAGCCGGTGCCGCAGGGGCAACCCGGCGAGCTATGGATCAGGGGGCCCGGCGTGATGCGCGGCTACTACCGCGCACCGGACCTGAGCGCCGAGGCCCTGCGGCCCGACGGCTGGCTCAATACCTG > GWNJ‑0478:712:GW2002102894th:2:1106:12414:59778/1‑150 (MQ=60)
CCGGAGGACAAGCCGGTGCCGCAGGGGCAACCCGGCGAGCTATGGATCAGGGGGCCCGGCGTGATGCGCGGCTACTACCGCGCACCGGACCTGAGCGCCGAGGCCCTGCGGCCCGACGGCTGGCTCAATACCGGCGACATCGGCCGGCTG > GWNJ‑0478:712:GW2002102894th:2:2103:8934:84575/1‑150 (MQ=60)
|
GCGATGTTCGGCCAGCCGCTGCACCATGGCTACGGCATGACCGAATATGCGGGGTCGCTGTTCGTGACCCGCATGGACCGGCCGCGCACCGATTGCTCGGCGGGCGAGATCGTCGAAGGTGCAGAGCTGCGGGTAGTCGGGCCGGAGGGCAAGCCGGTGCCGCAGGGGCAACCCGGCGAGCTATGGATCAGGGGGCCCGGCGTGATGCGCGGCTACTACCGCGCACCGGACCTGAGCGCCGAGGCCCTGCGGCCCGACGGCTGGCTCAATACCGGCGACATCGGCCGGCTG > AM260480/1537065‑1537355
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
