Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A0 F0 I1 R1
|
149 |
26.7 |
2337422 |
87.5% |
2045244 |
105.0 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
AM260479 |
1,071,935 |
+G |
coding (626/627 nt) |
h16_A0979 → |
Hypothetical protein |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | AM260479 | 1,071,934 | 1 | . | G | 100.0%
| 86.9
/ NA
| 23 | *209G (TGA→GGA) | h16_A0979 | Hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base . (0/0); new base G (12/11); total (12/11) |
TGGCCACGCGCAACCTCTACCGGGCCGAGGTGGGGTTGTCCGTCGCCGGCGACAACGCGGCCCTGCGCGCGCTGTGGCCGGGCTGGACCGGCTTCGGCATGCAGGACCACACCGTGCTGCTGACCAACGCCT‑GACGCCCGAGGGGCGCAACGGCACGCTCGCCGTGATCAAGGAAGCGGTGCCCACCAGCAA‑GGCGGCGTGATCGACACCGCGCTGACCGTCGGGCTGGCCGCCTTCGGGCCGGAAATCC > AM260479/1071803‑1072052
|
tGGCCACGCGCAACCTCTACCGGGCCGAGGTGGGGTTGTCCGTCGCCGGCGACAACGCGGCCCTGCGCGCGCTGTGGCCGGGCTGGACCGGCTTCGGCATGCAGGACCACACCGTGCTGCTGACCAACGCCT‑g > 1:184481/1‑133 (MQ=255)
aCCTCTACCGGGCCGAGGTGGGGTTGTCCGTCGCCGGCGACAACGCGGCCCTGCGCGCGCTGTGGCCGGGCTGGACCGGCTTCGGCATGCAGGACCACACCGTGCTGCTGACCAACGCCTGGACGCCCGAgggg > 2:992954/1‑134 (MQ=255)
aCCTCTACCGGGCCGAGGTGGGGTTGTCCGTCGCCGGCGACAACGCGGCCCTGCGCGCGCTGTGGCCGGGCTGGACCGGCTTCGGCATGCAGGACCACACCGTGCTGCTGACCAACGCCTGGACGCCCGAgggg < 1:350410/134‑1 (MQ=255)
gTGGGGTTGTCCGTCGCCGGCGACAACGCGGCCCTGCGCGCGCTGTGGCCGGGCTGGACCGGCTTCGGCATGCAGGACCACACCGTGCTGCTGACCAACGCCTGGACGCCCGAGGGGCGCAACGGCACGCTCGc < 1:359969/134‑1 (MQ=255)
ggggTTGTCCGTCGCCGGCGACAACGCGGCCCTGCGCGCGCTGTGGCCGGGCTGGACCGGCTTCGGCATGCAGGACCACACCGTGCTGCTGACCAACGCCTGGACGCCCGAGGGGCGCAACGGCACGCt < 2:811467/129‑1 (MQ=255)
ggggTTGTCCGTCGCCGGCGACAACGCGGCCCTGCGCGCGCTGTGGCCGGGCTGGACCGGCTTCGGCATGCAGGACCACACCGTGCTGCTGACCAACGCCTGGACGCCCGAGGGGCGCAACGGCACGCt > 1:811467/1‑129 (MQ=255)
gCGACAACGCGGCCCTGCGCGCGCTGTGGCCGGGCTGGACCGGCTTCGGCATGCAGGACCACACCGTGCTGCTGACCAACGCCTGGACGCCCGAGGGGCGCAACGGCACGCTCGCCGTGATCAAGGAAGCGGTg < 2:188502/134‑1 (MQ=255)
cTGCGCGCGCTGTGGCCGGGCTGGACCGGCTTCGGCATGCAGGACCACACCGTGCTGCTGACCAACGCCTGGACGCCCGAGTGGCGCAACGGCACGCTCGCCGTGATCAAGGAAGCGGTGCCCACCAGCAA‑ggg < 2:184481/134‑2 (MQ=255)
cgcgcTGTGGCCGGGCTGGACCGGCTTCGGCATGCAGGACCACACCGTGCTGCTGACCAACGCCTGGACGCCCGAGGGGCGCAACGGCACGCt > 1:371196/1‑93 (MQ=255)
cgcgcTGTGGCCGGGCTGGACCGGCTTCGGCATGCAGGACCACACCGTGCTGCTGACCAACGCCTGGACGCCCGAGGGGCGCAACGGCACGCt < 2:371196/93‑1 (MQ=255)
gtcgCCGGGCTGGACCGGCTTCGGCATGCAGGACCACACCGTGCTGCTGACCAACGCCTGGACGCCCGAGGGGCGCAACGGCACGCTCGCCGTGATCAAGGAAGCGGTGCCCACCAGCAAGGGCGGCGTGATc > 1:378016/4‑133 (MQ=255)
tgGCCGGGCTGGACCGGCTTCGGCATGCAGGACCACACCGTGCTGCTGACCAACGCCTGGACGCCCGAGGGGCGCAACGGCACGCTCGCCGTGATCAAGGa > 1:386246/1‑101 (MQ=255)
tgGCCGGGCTGGACCGGCTTCGGCATGCAGGACCACACCGTGCTGCTGACCAACGCCTGGACGCCCGAGGGGCGCAACGGCACGCTCGCCGTGATCAAGGa < 2:386246/101‑1 (MQ=255)
aCCGGCTTCGGCATGCAGGACCACACCGTGCTGCTGACCAACGCCTGGACGCCCGAGGGGCGCAACGGCACGCTCGCCGTGATc > 1:1140039/1‑84 (MQ=255)
aCCGGCTTCGGCATGCAGGACCACACCGTGCTGCTGACCAACGCCTGGACGCCCGAGGGGCGCAACGGCACGCTCGCCGTGATc < 2:1140039/84‑1 (MQ=255)
gCTTCGGCATGCAGGACCACACCGTGCTGCTGACCAACGCCTGGACGCCCGAGGGGCGCAACgg < 1:551239/64‑1 (MQ=255)
gCTTCGGCATGCAGGACCACACCGTGCTGCTGACCAACGCCTGGACGCCCGAGGGGCGCAACgg > 2:551239/1‑64 (MQ=255)
ccGTGCTGCTGACCAACGCCTGGACGCCCGAGGGGCGCAACGGCACGCTCGCCGTGATCAAGGAAGCGGTGCCCACCAGCAAGGGCGGCGTGATCGACACCGCGCTGACCGTCGGGCTGGCCGCCTTCGGGCCg > 2:954472/1‑134 (MQ=255)
gTGCTGCTGACCAACGCCTGGACGCCCGAGGGGCGCAACGGCACGCTCGCCGTGATCAAGGAAGCGGTGCCCACCAGCAAGGGCGGCGTGATCGACACCGCGCTGACCGTCGGGCTGGCCGCCTTCGGGCCgg > 2:41114/1‑133 (MQ=255)
gctgACCAACGCCTGGACGCCCGAGGGGCGCAACGGCACGCTCGCCGTGATCAAGGAAGCGGTGCCCACCAGCAAGGGCGGCGTGATCGACACcg > 2:1081563/1‑95 (MQ=255)
gctgACCAACGCCTGGACGCCCGAGGGGCGCAACGGCACGCTCGCCGTGATCAAGGAAGCGGTGCCCACCAGCAAGGGCGGCGTGATCGACACcg < 1:1081563/95‑1 (MQ=255)
gctgACCAACGCCTGGACGCCCGAGGGGCGCAACGGCACGCTCGCCGTGATCAAGGAAGCGGTGCCCACCAGCAAGGGCGGCGTGATCGACACCGCGCTGACCGTCGGGCTGGCCGCCTTCGGGCCGGAAATcc > 2:1137605/1‑134 (MQ=255)
tgACCAACGCCTGGACGCCCGAGGGGCGCAACGGCACGCt < 2:160906/40‑1 (MQ=38)
tgACCAACGCCTGGACGCCCGAGGGGCGCAACGGCACGCt > 1:160906/1‑40 (MQ=255)
|
TGGCCACGCGCAACCTCTACCGGGCCGAGGTGGGGTTGTCCGTCGCCGGCGACAACGCGGCCCTGCGCGCGCTGTGGCCGGGCTGGACCGGCTTCGGCATGCAGGACCACACCGTGCTGCTGACCAACGCCT‑GACGCCCGAGGGGCGCAACGGCACGCTCGCCGTGATCAAGGAAGCGGTGCCCACCAGCAA‑GGCGGCGTGATCGACACCGCGCTGACCGTCGGGCTGGCCGCCTTCGGGCCGGAAATCC > AM260479/1071803‑1072052
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
BRESEQ :: bam2aln output
GTGCCGGCTTCGACCTGGCCACGCGCAACCTCTACCGGGCCGAGGTGGGGTTGTCCGTCGCCGGCGACAACGCGGCCCTGCGCGCGCTGTGGCCGGGCTGGACCGGCTTCGGCATGCAGGACCACACCGTGCTGCTGACCAACGCCT‑GACGCCCGAGGGGCGCAACGGCACGCTCGCCGTGATCAAGGAAGCGGTGCCCACCAGCAA‑GGCGGCGTGATCGACACCGCGCTGACCGTCGGGCTGGCCGCCTTCGGGCCGGAAATCCTGAAACTGGACCTGGGCCGCATCCAG > AM260479/1071788‑1072078
|
GTGCCGGCTTCGACCTGGCCACGCGCAACCTCTACCGGGCCGAGGTGGGGTTGTCCGTCGCCGGCGACAACGCGGCCCTGCGCGCGCTGTGGCCGGGCTGGACCGGCTTCGGCATGCAGGACCACACCGTGCTGCTGACCAACGCCT‑GG > GWNJ‑0478:712:GW2002102894th:2:1110:18099:97689/1‑149 (MQ=60)
AACCTCTACCGGGCCGAGGTGGGGTTGTCCGTCGCCGGCGACAACGCGGCCCTGCGCGCGCTGTGGCCGGGCTGGACCGGCTTCGGCATGCAGGACCACACCGTGCTGCTGACCAACGCCTGGACGCCCGAGGGGCGCAACGGCACGCTC < GWNJ‑0478:712:GW2002102894th:2:1204:14970:21815/150‑1 (MQ=60)
CTACCGGGCCGAGGTGGGGTTGTCCGTCGCCGGCGACAACGCGGCCCTGCGCGCGCTGTGGCCGGGCTGGACCGGCTTCGGCATGCAGGACCACACCGTGCTGCTGACCAACGCCTGGACGCCCGAGGGGCGCAACGGCACGCTCGCCGT > GWNJ‑0478:712:GW2002102894th:2:2115:9106:30218/1‑150 (MQ=60)
acggcatacgagattagcgagtgtctcgtgggctcggagatgtgtataagagacagGGCCGGGCTGGACCGGCTTCGGCATGCAGGACCACACCGTGCTGCTGACCAACGCCTGGACGCCCGAGGGGCGCAACGGCACGCTCGCCGTGAT < GWNJ‑0478:712:GW2002102894th:2:1214:19134:90309/94‑1 (MQ=60)
GGCCGAGGTGGGGTTGTCCGTCGCCGGCGACAACGCGGCCCTGCGCGCGCTGTGGCCGGGCTGGACCGGCTTCGGCATGCAGGACCACACCGTGCTGCTGACCAACGCCT‑Ggacctgtctcttatacacatctccgagcccacgagacac > GWNJ‑0478:712:GW2002102894th:2:2115:17570:27876/1‑111 (MQ=60)
GGTGGGGTTGTCCGTCGCCGGCGACAACGCGGCCCTGCGCGCGCTGTGGCCGGGCTGGACCGGCTTCGGCATGCAGGACCACACCGTGCTGCTGACCAACGCCTGGACGCCCGAGGGGCGCAACGGCACGCTCGCCGTGATCAAGGAAGC < GWNJ‑0478:712:GW2002102894th:2:1204:3876:81105/150‑1 (MQ=60)
tttcaagcagaagacggcatacgagattagcgagtgtctcgtgggctcggagatgtgtataagagacagGTGCTGCTGACCAACGCCTGGACGCCCGAGGGGCGCAACGGCACGCTCGCCGTGATCAAGGAAGCGGTGCCCACCAGCAA < GWNJ‑0478:712:GW2002102894th:2:2209:19392:68601/80‑1 (MQ=60)
gcttttttttttttttttcaagcagaagacggcatacgagattagcgagtgtctcgtgggctcggagatgtgtataagagacagcctgGACGCCCGAGGGGCGCAACGGCACGCTCGCCGTGATCAAGGAAGCGGTGCCCACCAGCAA‑GG < GWNJ‑0478:712:GW2002102894th:2:1202:7119:27589/62‑1 (MQ=60)
ttttttttttttttttcaagcagaagacggcatacgagattagcgagtgtctcgtgggctcggagatgtgtataagagacagcctgGACGCCCGAGGGGCGCAACGGCACGCTCGCCGTGATCAAGGAAGCGGTGCCCACCAGCAA‑GGG < GWNJ‑0478:712:GW2002102894th:2:2104:15144:83897/63‑1 (MQ=60)
CAACGCGGCCCTGCGCGCGCTGTGGCCGGGCTGGACCGGCTTCGGCATGCAGGACCACACCGTGCTGCTGACCAACGCCTGGACGCCCGAGGGGCGCAACGGCACGCTCGCCGTGATCAAGGActgtctcttatacacatctccgagccc > GWNJ‑0478:712:GW2002102894th:2:1207:8572:47795/1‑123 (MQ=60)
gcgcagggccgcgttgtcGCCGGGCTGGACCGGCTTCGGCATGCAGGACCACACCGTGCTGCTGACCAACGCCTGGACGCCCGAGGGGCGCAACGGCACGCTCGCCGTGATCAAGGAAGCGGTGCCCACCAGCAAGGGCGGCGTGATCG > GWNJ‑0478:712:GW2002102894th:2:1207:12752:87653/19‑149 (MQ=60)
GCCCTGCGCGCGCTGTGGCCGGGCTGGACCGGCTTCGGCATGCAGGACCACACCGTGCTGCTGACCAACGCCTGGACGCCCGAGGGGCGCAACGGCACGCTCGCCGTGATCAAGGAAGCGGTGCCCACCAGCtgtctctttacacatctc > GWNJ‑0478:712:GW2002102894th:2:1206:8440:36932/1‑132 (MQ=60)
CTGTGGCCGGGCTGGACCGGCTTCGGCATGCAGGACCACACCGTGCTGCTGACCAACGCCTGGACGCCCGAGGGGCGCAACGGCACGCTCGCCGTGATCAAGGAAGCGGTGCCCctgtctcttatacacatctccgagcccacgagacac > GWNJ‑0478:712:GW2002102894th:2:2215:20035:76220/1‑114 (MQ=60)
gctcggagatgtgtataagagacagGGACCACACCGTGCTGCTGACCAACGCCTGGACGCCCGAGGGGCGCAACGGCACGCTCGCCGTGATCAAGGAAGCGGTGCCCACCAGCAAGGGCGGCGTGATCGACACCGCGCTGACCGTCGGGC < GWNJ‑0478:712:GW2002102894th:2:2213:12660:7094/125‑1 (MQ=60)
ACCACACCGTGCTGCTGACCAACGCCTGGACGCCCGAGGGGCGCAACGGCACGCTCGCCGTGATCAAGGActgtctcttatacacatctccgagcccacgagacactcgctaatctcgtatgccgtcttctgcttgaaaaaaaaaaaa > GWNJ‑0478:712:GW2002102894th:2:1111:17101:68844/1‑70 (MQ=60)
CTGCTGACCAACGCCTGGACGCCCGAGGGGCGCAACGGCACGCTCGCCGTGATC‑AGGAAGCGGTGCCCACCAGCAAGGGCGGCGTGATCGACACCGCGCTGACCGTCGGGCTGGCCGCCTTCGGGCCGGAAATCCTGAAACTGGACCTGG > GWNJ‑0478:712:GW2002102894th:2:1104:19199:55911/1‑150 (MQ=60)
GCCTGGACGCCCGAGGGGCGCAACGGCACGCTCGCCGTGATCAAGGAAGCGGTGCCCACCAGCAAGGGCGGCGTGATCGACACCGCGCTGACCGTCGGGCTGGCCGCCTTCGGGCCGGAAATCCTGAAACTGGACCTGGGCCGCATCCAG < GWNJ‑0478:712:GW2002102894th:2:1210:13327:37630/150‑1 (MQ=60)
|
GTGCCGGCTTCGACCTGGCCACGCGCAACCTCTACCGGGCCGAGGTGGGGTTGTCCGTCGCCGGCGACAACGCGGCCCTGCGCGCGCTGTGGCCGGGCTGGACCGGCTTCGGCATGCAGGACCACACCGTGCTGCTGACCAACGCCT‑GACGCCCGAGGGGCGCAACGGCACGCTCGCCGTGATCAAGGAAGCGGTGCCCACCAGCAA‑GGCGGCGTGATCGACACCGCGCTGACCGTCGGGCTGGCCGCCTTCGGGCCGGAAATCCTGAAACTGGACCTGGGCCGCATCCAG > AM260479/1071788‑1072078
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
