Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A0 F0 I1 R1
|
149 |
26.7 |
2337422 |
87.5% |
2045244 |
105.0 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
AM260479 |
1,354,645 |
+C |
intergenic (+98/‑15) |
h16_A1248 → / → h16_A1249 |
predicted signal transduction protein containingCHASE, PAS, GGDEF and EAL domains/Hypothetical protein |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | AM260479 | 1,354,643 | 1 | . | C | 100.0%
| 89.3
/ NA
| 24 | intergenic (+96/‑17) | h16_A1248/h16_A1249 | predicted signal transduction protein containingCHASE, PAS, GGDEF and EAL domains/Hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base . (0/0); new base C (14/10); total (14/10) |
GCTCTCGAGCATGGACCGCAACCGATGCGCGCCTGACTGCCCGTCAGTCCGGTGCGTGCTTAAGCAGTCCAATAACAGGGAGCTAGATCATGCAGGACCGTACTATTGAAATCATGTGGCAGGCCATCGACG‑CCTGGACTTTTCCAGGATGAAGGCCAAGCTGCTGCATCAGAAGCATGCGCATTGGTCGCCGGAATCGCTGGAGCAGGCGGAGAGCGGCTACCGGCAATTCCTCAAGCTGGCGGC > AM260479/1354512‑1354757
|
gCTCTCGAGCATGGACCGCAACCGATGCGCGCCTGACTGCCCGTCAGTCCGGTGCGTGCTTAAGCAGTCCAATAACAGGGAGCTAGATCATGCAGGACCGTACTATTGAAATCATGTGGCAGGCCATCGACG‑cc > 1:380107/1‑134 (MQ=255)
cAACCGATGCGCGCCTGACTGCCCGTCAGTCCGGTGCGTGCTTAAGCAGTCCAATAACAGGGAGCTAGATCATGCAGGACCGTACTATTGAAATCATGTGGCAGGCCATCGACGCCCTGGACTTTTCCAGGAt > 1:554465/1‑133 (MQ=255)
gcgtgcTTAAGCAGTCCAATAACAGGGAGCTAGATCATGCAGGACCGTACTATTGAAATCATGTGGCAGGCCATCGACGCCCTGGACTTTTCCAGGATGAAGGCCAAgct > 2:291663/1‑110 (MQ=255)
gcgtgcTTAAGCAGTCCAATAACAGGGAGCTAGATCATGCAGGACCGTACTATTGAAATCATGTGGCAGGCCATCGACGCCCTGGACTTTTCCAGGATGAAGGCCAAgct < 1:291663/110‑1 (MQ=255)
aaGCAGTCCAATAACAGGGAGCTAGATCATGCAGGACCGTACTATTGAAATCATGTGGCAGGCCATCGACGCCCTGGACTTTTCCAGGATGAAGGCCAAg < 2:899368/100‑1 (MQ=255)
aaGCAGTCCAATAACAGGGAGCTAGATCATGCAGGACCGTACTATTGAAATCATGTGGCAGGCCATCGACGCCCTGGACTTTTCCAGGATGAAGGCCAAg > 2:804621/1‑100 (MQ=255)
aaGCAGTCCAATAACAGGGAGCTAGATCATGCAGGACCGTACTATTGAAATCATGTGGCAGGCCATCGACGCCCTGGACTTTTCCAGGATGAAGGCCAAg < 1:804621/100‑1 (MQ=255)
aaGCAGTCCAATAACAGGGAGCTAGATCATGCAGGACCGTACTATTGAAATCATGTGGCAGGCCATCGACGCCCTGGACTTTTCCAGGATGAAGGCCAAg > 1:899368/1‑100 (MQ=255)
gTCCAATAACAGGGAGCTAGATCATGCAGGACCGTACTATTGAAATCATGTGGCAGGCCATCGACGCCCTGGACTTTTCCAGGATGAAGGCCAAGCTGCTGCAt > 1:1148671/1‑104 (MQ=255)
gTCCAATAACAGGGAGCTAGATCATGCAGGACCGTACTATTGAAATCATGTGGCAGGCCATCGACGCCCTGGACTTTTCCAGGATGAAGGCCAAGCTGCTGCAt < 2:1148671/104‑1 (MQ=255)
gTCCAATAACAGGGAGCTAGATCATGCAGGACCGTACTATTGAAATCATGTGGCAGGCCATCGACGCCCTGGACTTTTCCAGGATGAAGGCCAAGCTGCTGCATCAGAAGCATGCGCATTGGTCGCCGGAATCg > 1:1115372/1‑134 (MQ=255)
tAACAGGGAGCTAGATCATGCAGGACCGTACTATTGAAATCATGTGGCAGGCCATCGACGCCCTGGACTTTTCCAGGATGAAGGCCAAg < 2:908219/89‑1 (MQ=255)
tAACAGGGAGCTAGATCATGCAGGACCGTACTATTGAAATCATGTGGCAGGCCATCGACGCCCTGGACTTTTCCAGGATGAAGGCCAAg > 1:908219/1‑89 (MQ=255)
gCTAGATCATGCAGGACCGTACTATTGAAATCATGTGGCAGGCCATCGACGCCCTGGACTTTTCCAGGATGAAGGCCAAGCTGCTGCATCAGAAGCATGCGCATTGGTCGCCGGAATCGCTGGAGCAGGCg > 2:152738/1‑131 (MQ=255)
cTAGATCATGCAGGACCGTACTATTGAAATCATGTGGCAGGCCATCGACGCCCTGGACTTTTCCAGGATGAAGGCCAAGCTGCTGCATCAGAAGCATGCGCATTGGTCGCCGGAATCGCTGGAGCAGGCGgaga < 2:554465/134‑1 (MQ=255)
ggACCGTACTATTGAAATCATGTGGCAGGCCATCGACGCCCTGGACTTTTCCAGGATGAAGGCCAAGCTGCTGCATCAGAAGCATGCGCATTGGTCGCCGGAATCGCTGGAGCAGGCGGAGAGCGGCTACCgg > 1:721900/1‑133 (MQ=255)
aCTATTGAAATCATGTGGCAGGCCATCGACGCCCTGGACTTTTCCAGGATGAAGGCCAAGCTGCTGCAt > 2:191808/1‑69 (MQ=255)
aCTATTGAAATCATGTGGCAGGCCATCGACGCCCTGGACTTTTCCAGGATGAAGGCCAAGCTGCTGCAt < 1:191808/69‑1 (MQ=255)
aTTGAAATCATGTGGCAGGCCATCGACGCCCTGGACtt < 2:1009800/38‑1 (MQ=25)
aTTGAAATCATGTGGCAGGCCATCGACGCCCTGGACtt > 1:1009800/1‑38 (MQ=25)
aTTGAAATCATGTGGCAGGCCATCGACGCCCTGGACTTTTCCAGGATGAAGGCCAAGCTGCTGCATCAGAAGCATGCGCATTGGTCGc > 2:197535/1‑88 (MQ=255)
aTTGAAATCATGTGGCAGGCCATCGACGCCCTGGACTTTTCCAGGATGAAGGCCAAGCTGCTGCATCAGAAGCATGCGCATTGGTCGc < 1:197535/88‑1 (MQ=255)
tgtgGCAGGCCATCGACGCCCTGGACTTTTCCAGGATGAAGGCCAAGCTGCTGCATCAGAAGCATGCGCATTGGTCGCCGGAATCGCTGGAGCAGGCGGAGAGCGGCTACCGGCAATTCCTCAAGCTggcggc > 2:719941/1‑133 (MQ=255)
ggCAGGCCATCGACGCCCTGGACTTTTCCAGGATGAAGGCCAAGCTGCTGCATCAGAAGCATGCGCATTGGTCGCCGGAATCGCTGGAGCAGGCGGAGAGCGGCTACCGGCAATTCCTCAAGCTggcggc > 2:143195/1‑130 (MQ=255)
ggCAGGCCATCGACGCCCTGGACTTTTCCAGGATGAAGGCCAAGCTGCTGCATCAGAAGCATGCGCATTGGTCGCCGGAATCGCTGGAGCAGGCGGAGAGCGGCTACCGGCAATTCCTCAAGCTggcggc < 1:143195/130‑1 (MQ=255)
|
GCTCTCGAGCATGGACCGCAACCGATGCGCGCCTGACTGCCCGTCAGTCCGGTGCGTGCTTAAGCAGTCCAATAACAGGGAGCTAGATCATGCAGGACCGTACTATTGAAATCATGTGGCAGGCCATCGACG‑CCTGGACTTTTCCAGGATGAAGGCCAAGCTGCTGCATCAGAAGCATGCGCATTGGTCGCCGGAATCGCTGGAGCAGGCGGAGAGCGGCTACCGGCAATTCCTCAAGCTGGCGGC > AM260479/1354512‑1354757
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
BRESEQ :: bam2aln output
ATGGAGAACGGTCCCGCTCTCGAGCATGGACCGCAACCGATGCGCGCCTGACTGCCCGTCAGTCCGGTGCGTGCTTAAGCAGTCCAATAACAGGGAGCTAGATCATGCAGGACCGTACTATTGAAATCATGTGGCAGGCCATCGACG‑CCTGGACTTTTCCAGGATGAAGGCCAAGCTGCTGCATCAGAAGCATGCGCATTGGTCGCCGGAATCGCTGGAGCAGGCGGAGAGCGGCTACCGGCAATTCCTCAAGCTGGCGGCGAAGCATCCGGACAC > AM260479/1354497‑1354772
|
ATGGAGAACGGTCCCGCTCTCGAGCATGGACCGCAACCGATGCGCGCCTGACTGCCCGTCAGTCCGGTGCGTGCTTAAGCAGTCCAATAACAGGGAGCTAGATCATGCAGGACCGTACTATTGAAATCATGTGGCAGGCCATCGACG‑CCC > GWNJ‑0478:712:GW2002102894th:2:1207:6267:99770/1‑150 (MQ=60)
gtgtctttttttttttttcaagcagaagacggcatacgagattagcgagtgtctcgtgggctcggagatgtgtataagagacaGGAGCTAGATCATGCAGGACCGTACTATTGAAATCATGTGGCAGGCCATCGACGCCCTGGACTTTTC < GWNJ‑0478:712:GW2002102894th:2:2115:10763:51455/67‑1 (MQ=60)
CTCGAGCATGGACCGCAACCGATGCGCGCCTGACTGCCCGTCAGTCCGGTGCGTGCTTAAGCAGTCCAATAACAGGGAGCTAGATCATGCAGGACCGTACTATTGAAATCATGTGGCAGGCCATCGACGCCCTGGACTTTTCCAGGATG > GWNJ‑0478:712:GW2002102894th:2:1216:12590:5410/1‑149 (MQ=60)
gagatgtgtataagagacagGTCCGGTGCGTGCTTAAGCAGTCCAATAACAGGGAGCTAGATCATGCAGGACCGTACTATTGAAATCATGTGGCAGGCCATCGACGCCCTGGACTTTTCCAGGATGAAGGCCAAGCTGCTGCATCAGAAG < GWNJ‑0478:712:GW2002102894th:2:2112:2511:97993/130‑1 (MQ=60)
gctaacttcttcttttttttttcaagcagaagacggcatacgagattagcgagtgtctcgtgggctcggagatgtgtataagagacagGTGGCAGGCCATCGACGCCCTGGACTTTTCCAGGATGAAGGCCAAGCTGCTGCATCAGAAG < GWNJ‑0478:712:GW2002102894th:2:1105:6176:51732/61‑1 (MQ=60)
aagagacagGCCCGTCAGTCCGGTGCGTGCTTAAGCAGTCCAATAACAGGGAGCTAGATCATGCAGGACCGTACTATTGAAATCATGTGGCAGGCCATCGACGCCCTGGACTTTTCCAGGATGAAGGCCAAGCTGCTGCATCAGAAGCA < GWNJ‑0478:712:GW2002102894th:2:1201:6951:48675/140‑1 (MQ=60)
atacgagattagcgagtgtctcgtgggctcggagatgtgtataagagacAGATCATGCAGGACCGTACTATTGAAATCATGTGGCAGGCCATCGACGCCCTGGACTTTTCCAGGATGAAGGCCAAGCTGCTGCATCAGAAGCATGCGCAT < GWNJ‑0478:712:GW2002102894th:2:1113:5721:35638/101‑1 (MQ=60)
GTCCGGTGCGTGCTTAAGCAGTCCAATAACAGGGAGCTAGATCATGCAGGACCGTACTATTGAAATCATGTGGCAGGCCATCGACGCCCTGGACTTTTCCAGGATGAAGGCCAAGCTGCTGCATCAGAAGCtgtctcttatacacatctc > GWNJ‑0478:712:GW2002102894th:2:2203:10973:87524/1‑131 (MQ=60)
GTGCGTGCTTAAGCAGTCCAATAACAGGGAGCTAGATCATGCAGGACCGTACTATTGAAATCATGTGGCAGGCCATCGACGCCCTGGACTTTTCCAGGATGAAGGCCAAGCTGCTGCATCAGAAGCATGCGCATctgtctcttatacac > GWNJ‑0478:712:GW2002102894th:2:2216:15629:13955/1‑134 (MQ=60)
GTGCGTGCTTAAGCAGTCCAATAACAGGGAGCTAGATCATGCAGGACCGTACTATTGAAATCATGTGGCAGGCCATCGACGCCCTGGACTTTTCCAGGATGAAGGCCAAGCTGCTGCATCAGAAGCATGCGCATTGGTCGCCGGAATCGC > GWNJ‑0478:712:GW2002102894th:2:2214:19240:65972/1‑150 (MQ=60)
TGCTTAAGCAGTCCAATAACAGGGAGCTAGATCATGCAGGACCGTACTATTGAAATCATGTGGCAGGCCATCGACGCCCTGGACTTTTCCtgtctcttatacacatctccgagcccacgagacactcgctaatctcgtatgccgtcttct > GWNJ‑0478:712:GW2002102894th:2:1205:4367:38664/1‑90 (MQ=60)
GCTTAAGCAGTCCAATAACAGGGAGCTAGATCATGCAGGACCGTACTATTGAAATCATGTGGCAGGCCATCGACGCCCTGGACTTTTCCAGGATGAAGGCCAAGCTGCTGCATCAGAAGCtgtctcttatacacatctccgagcccacg > GWNJ‑0478:712:GW2002102894th:2:2202:9706:43052/1‑120 (MQ=60)
cgtgggctcggagatgtgtataagagacagATGCAGGACCGTACTATTGAAATCATGTGGCAGGCCATCGACGCCCTGGACTTTTCCAGGATGAAGGCCAAGCTGCTGCATCAGAAGCATGCGCATTGGTCGCCGGAATCGCTGGAGC < GWNJ‑0478:712:GW2002102894th:2:1113:15725:65237/118‑1 (MQ=60)
GAGCTAGATCATGCAGGACCGTACTATTGAAATCATGTGGCAGGCCATCGACGCCCTGGACTTTTCCAGGATGAAGGCCAAGCTGCTGCATCAGAAGCATGCGCATTGGTCGCCGGAATCGCTGGAGCAGGCGGAGAGCGGCTACCGGC > GWNJ‑0478:712:GW2002102894th:2:2109:20743:77426/1‑149 (MQ=60)
ATGCAGGACCGTACTATTGAAATCATGTGGCAGGCCATCGACGCCCTGGACTTTTCCAGGATGAAGGCCtgtctcttatacacatctccgagcccacgagacactcgctaatctcgtatgccgtcttctgcttgaaaaaaaaaaaaaac > GWNJ‑0478:712:GW2002102894th:2:2208:3794:32701/1‑69 (MQ=60)
CATGTGGCAGGCCATCGACGCCCTGGACTTTTCCAGGATGAAGGCCAAGCTGCTGCATCAGAAGCATGCGCATTGGTCGCCGGAATCGCTGGAGCAGGCGGAGAGCGGCTACCGGCAATTCCTCAAGCTGGCGGCGAAGCATCCGGACAC < GWNJ‑0478:712:GW2002102894th:2:1109:4286:74730/150‑1 (MQ=60)
|
ATGGAGAACGGTCCCGCTCTCGAGCATGGACCGCAACCGATGCGCGCCTGACTGCCCGTCAGTCCGGTGCGTGCTTAAGCAGTCCAATAACAGGGAGCTAGATCATGCAGGACCGTACTATTGAAATCATGTGGCAGGCCATCGACG‑CCTGGACTTTTCCAGGATGAAGGCCAAGCTGCTGCATCAGAAGCATGCGCATTGGTCGCCGGAATCGCTGGAGCAGGCGGAGAGCGGCTACCGGCAATTCCTCAAGCTGGCGGCGAAGCATCCGGACAC > AM260479/1354497‑1354772
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
