Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A0 F0 I1 R1
|
149 |
26.7 |
2337422 |
87.5% |
2045244 |
105.0 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
AM260479 |
2,111,013 |
+G |
coding (553/1089 nt) |
h16_A1942 → |
Hypothetical protein |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | AM260479 | 2,111,012 | 1 | . | G | 100.0%
| 45.5
/ NA
| 13 | D184E (GAT→GAG) | h16_A1942 | Hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base . (0/0); new base G (5/8); total (5/8) |
TGGTGGGACCGATCGCCAGCATGATTCCTCTTCCACAAGCGCAGTTGATCGGGCGCATCGCCAATGTGGCCGGGCGCATGCTCGCCGACGGCGCCGACGAGTATGAGACCTTCGACGAACTGGTGGAT‑GACTCGACGA‑GATGCGATCGACGCCGCCGCGCCGGTGCTGGCCGGCATGGTGCTCCGCCGCGCCCTCCCCTCGGTCGCGCGCGCCGCGCCCGCCGTGCGTCGCGCCGCGGTGCGCGG > AM260479/2110885‑2111129
|
tggtggGACCGATCGCCAGCATGATTCCTCTTCCACAAGCGCAGTTGATCGGGCGCATCGCCAATGTGGCCGGGCGCATGCTCGCCGACGGCGCCGACGAGTATGAGACCTTCGACGAACTGGTGGAT‑ggact < 1:70301/133‑5 (MQ=255)
ccAGCATGATTCCTCTTCCACAAGCGCAGTTGATCGGGCGCATCGCCAATGTGGCCGGGCGCATGCTCGCCGACGGCGCCGACGAGTATGAGACCTTCGACGAACTGGTGGATGGACTCGACGAGGATGCGATc > 2:910768/1‑134 (MQ=255)
cAGCATGATTCCTCTTCCACAAGCGCAGTTGATCGGGCGCATCGCCAATGTGGCCGGGCGCATGCTCGCCGACGGCGCCGACGAGTATGAGACCTTCGACGAACTGGTGGATGGACTCGACGAGGATGCGATc > 2:155687/1‑133 (MQ=255)
cATGATTCCTCTTCCACAAGCGCAGTTGATCGGGCGCATCGCCAATGTGGCCGGGCGCATGCTCGCCGACGGCGCCGACGAGTATGAGACCTTCGACGAACTGGTGGATGGACTCGACGAGGATGCGATCGAcg < 2:39478/134‑1 (MQ=255)
ccACAAGCGCAGTTGATCGGGCGCATCGCCAATGTGGCCGGGCGCATGCTCGCCGACGGCGCCGACGAGTATGAGACCTTCGACGAACTGGTGGATGGACTCGACGAGGATGCGATCGACGCcgccgcgcc < 1:910768/131‑1 (MQ=255)
aTCGCCAATGTGGCCGGGCGCATGCTCGCCGACGGCGCCGACGAGTATGAGACCTTCGACGAACTGGTGGATGGACTCGACGAGGATGCGATCGACGCCGCCGCGCCGGTGCTGGCCGGCATGGTGCTccgccg < 2:224849/134‑1 (MQ=255)
cGCCAATGTGGCCGGGCGCATGCTCGCCGACGGCGCCGACGAGTATGAGACCTTCGACGAACTGGTGGATGGACTcg > 1:662677/1‑77 (MQ=255)
cGCCAATGTGGCCGGGCGCATGCTCGCCGACGGCGCCGACGAGTATGAGACCTTCGACGAACTGGTGGATGGACTcg < 2:662677/77‑1 (MQ=255)
cgacgaACTGGTGGATGGACTCGACGAGGATGCGATCGACGCCGCCGCGCCGGTGCTGGCCGGCATGGTGCTCCGCCGCGCCCTCCCCTCGGTCGCGCGCGCCGCGCCCGCCGTGCGTCGCGCCGCGGTgcgc < 1:120138/133‑1 (MQ=255)
cgacgaACTGGTGGATGGACTCGACGAGGATGCGATCGACGCCGCCGCGCCGGTGCTGGCCGGCATGGTGCTCCGCCGCGCCCTCCCCTCGGTCGCGCGCGCCGCGCCCGCCGTGCGTCGCGCCGCGGTgcg < 1:155687/132‑1 (MQ=255)
gacgaACTGGTGGATGGACTCGACGAGGATGCGATCGACGCCGCCGCGCCGGTGCTGGCCGGCATGGTGCTCCGCCGCGCCCTCCCCTCggg > 1:224484/1‑91 (MQ=255)
gacgaACTGGTGGATGGACTCGACGAGGATGCGATCGACGCCGCCGCGCCGGTGCTGGCCGGCATGGTGCTCCGCCGCGCCCTCCCCTCggg < 2:224484/92‑2 (MQ=255)
cgaACTGGTGGATGGACTCGACGAGGATGCGATCGACGCCGCCGCGCCGGTGCTGGCCGGCATGGTGCTCCGCCGCGCCCTCCCCTCGGTCGCGCGCGCCGCGCCCGCCGTGCGTCGCGCCGCGGTGCgcgg < 1:158722/132‑1 (MQ=255)
cgaACTGGTGGATGGACTCGACGAGGATGCGATCGACGCCGCCGCGCCGGTGCTGGCCGGCATGGTGCTCCGCCGCGCCCTCCCCTCGGTCGCGCGCGCCGCGCCCGCCGTGCGTCGCGCCGCGGTGCgcgg > 2:158722/1‑132 (MQ=255)
|
TGGTGGGACCGATCGCCAGCATGATTCCTCTTCCACAAGCGCAGTTGATCGGGCGCATCGCCAATGTGGCCGGGCGCATGCTCGCCGACGGCGCCGACGAGTATGAGACCTTCGACGAACTGGTGGAT‑GACTCGACGA‑GATGCGATCGACGCCGCCGCGCCGGTGCTGGCCGGCATGGTGCTCCGCCGCGCCCTCCCCTCGGTCGCGCGCGCCGCGCCCGCCGTGCGTCGCGCCGCGGTGCGCGG > AM260479/2110885‑2111129
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
BRESEQ :: bam2aln output
AGGCCGCGCGTGTGGTGGGACCGATCGCCAGCATGATTCCTCTTCCACAAGCGCAGTTGATCGGGCGCATCGCCAATGTGGCCGGGCGCATGCTCGCCGACGGCGCCGACGAGTATGAGACCTTCGACGAACTGGTGGAT‑GACTCGACGA‑GATGCGATCGACGCCGCCGCGCCGGTGCTGGCCGGCATGGTGCTCCGCCGCGCCCTCCCCTCGGTCGCGCGCGCCGCGCCCGCCGTGCGTCGCGCCGCGGTGCGCGGCGTGGCCCAGGCGGTGCGAA > AM260479/2110873‑2111149
|
aagacggcatacgagattagcgagtgtctcgtgggctcggagatgtgtataagagacaGATCGGGCGCATCGCCAATGTGGCCGGGCGCATGCTCGCCGACGGCGCCGACGAGTATGAGACCTTCGACGAACTGGTGGATGGACTCGACG < GWNJ‑0478:712:GW2002102894th:2:2215:13580:50507/92‑1 (MQ=60)
aagagacagGTGGTGGGGCCGATCGCCAGCATGATTCCTCTTCCACAAGCGCAGTTGATCGGGCGCATCGCCAATGTGGCCGGGCGCATGCTCGCCGACGGCGCCGACGAGTATGAGACCTTCGACGAACTGGTGGATGGACTCGACGA‑G < GWNJ‑0478:712:GW2002102894th:2:1201:11582:65440/141‑1 (MQ=60)
tattttttctttgatgtctttttttttttttttttttcaagcagaagacggcatacgagattagcgagtgtctcgtgggctcggagatgtgtataagagacagGAGTATGAGACCTTCGACGAACTGGTGGATGGACTCGACGAGGATG < GWNJ‑0478:712:GW2002102894th:2:2203:16727:72958/46‑1 (MQ=60)
aagacggcatacgagattagcgagtgtctcgtgggctcggagatgtgtataagagacagTCGCCAATGTGGCCGGGCGCATGCTCGCCGACGGCGCCGACGAGTATGAGACCTTCGACGAACTGGTGGATGGACTCGACGAGGATGCGAT < GWNJ‑0478:712:GW2002102894th:2:1112:13867:30326/91‑1 (MQ=60)
GTGGTGGGACCGATCGCCAGCATGATTCCTCTTCCACAAGCGCAGTTGATCGGGCGCATCGCCAATGTGGCCGGGCGCATGCTCGCCGACGGCGCCGACGAGTATGAGACCTTCGACGAACTGGTGGATGGACTCGACGAGGATGCGAT < GWNJ‑0478:712:GW2002102894th:2:1104:2677:48901/149‑1 (MQ=60)
TCCACAAGCGCAGTTGATCGGGCGCATCGCCAATGTGGCCGGGCGCATGCTCGCCGACGGCGCCGACGAGTATGAGACCTTCGACGAACTGGTGGATGGACTCGACGAGGATGCGATCGACGCCGCCGCGCCGGTGCTGGCCGGCAT < GWNJ‑0478:712:GW2002102894th:2:2202:3083:59445/147‑1 (MQ=60)
GTTGATCGGGCGCATCGCCAATGTGGCCGGGCGCATGCTCGCCGACGGCGCCGACGAGTATGAGACCTTCGACGAACTGGTGGATGGACTCGACGAGGATGCGATCGctgtctcttatacacatctccgagcccacgagacactcgcta > GWNJ‑0478:712:GW2002102894th:2:2107:21174:41692/1‑107 (MQ=60)
GGCGCATGCTCGCCGACGGCGCCGACGAGTATGAGACCTTCGACGAACTGGTGGATGGACTCGACGA‑Gctgtctcttatacacatctccgagcccacgagacactcgctaatctcgtatgccgtcttctgcttgaaaaaaaaaaaaaaa > GWNJ‑0478:712:GW2002102894th:2:2107:9036:91319/1‑68 (MQ=60)
GAGTATGAGACCTTCGACGAACTGGTGGATGGACTCGACGAGGATGCGATCGACGCCGCCGCGCCGGTGCTGGCCGGCATGGTGCTCCGCCGCGCCCTCCCCTCGGgcgcggcgcgcgcgacctgtctcttatacacatctccgagccc > GWNJ‑0478:712:GW2002102894th:2:1115:18286:5791/1‑106 (MQ=60)
GTATGAGACCTTCGACGAACTGGTGGATGGACTCGACGctgtctcttatacacatctccgagcccacgagacactcgctaatctcgtatgccgtcttctgcttgaaaaaaaaaaaaaaaagaaagaatgaagcacagagactagcata > GWNJ‑0478:712:GW2002102894th:2:2203:8272:41975/1‑38 (MQ=56)
TCGACGAACTGGTGGATGGACTCGACGAGGATGCGATCGACGCCGCCGCGCCGGTGCTGGCCGGCATGGTGCTCCGCCGCGCCCTCCCCTCGGTCGCGCGCGCCGCGCCCGCCGTGCGTCGCGCCGCGGTGCGCGGCGTGGCCCAGGCG < GWNJ‑0478:712:GW2002102894th:2:1108:10790:43857/149‑1 (MQ=60)
TCGACGAACTGGTGGATGGACTCGACGAGGATGCGATCGACGCCGCCGCGCCGGTGCTGGCCGGCATGGTGCTCCGCCGCGCCCTCCCCTCGGTCGCGCGCGCCGCGCCCGCCGTGCGTCGCGCCGCGGTGCGCGGCGTGGCCCAGGC < GWNJ‑0478:712:GW2002102894th:2:1111:11759:41642/148‑1 (MQ=60)
CGACGAACTGGTGGATGGACTCGACGAGGATGCGATCGACGCCGCCGCGCCGGTGCTGGCCGGCATGGTGCTCCGCCGCGCCCTCCCCTCGGTCGCGCGCGCCGCGCCCGCCGTGCGTCGCGCCGCGGTGCGCGGCGcgcgcgaccgagg < GWNJ‑0478:712:GW2002102894th:2:1111:6487:57489/150‑14 (MQ=60)
AACTGGTGGATGGACTCGACGAGGATGCGATCGACGCCGCCGCGCCGGTGCTGGCCGGCATGGTGCTCCGCCGCGCCCTCCCCTCGGTCGCGCGCGCCGCGCCCGCCGTGCGTCGCGCCGCGGTGCGCGGCGTGGCCCAGGCGGTGCGgg > GWNJ‑0478:712:GW2002102894th:2:2111:17275:2858/1‑148 (MQ=60)
|
AGGCCGCGCGTGTGGTGGGACCGATCGCCAGCATGATTCCTCTTCCACAAGCGCAGTTGATCGGGCGCATCGCCAATGTGGCCGGGCGCATGCTCGCCGACGGCGCCGACGAGTATGAGACCTTCGACGAACTGGTGGAT‑GACTCGACGA‑GATGCGATCGACGCCGCCGCGCCGGTGCTGGCCGGCATGGTGCTCCGCCGCGCCCTCCCCTCGGTCGCGCGCGCCGCGCCCGCCGTGCGTCGCGCCGCGGTGCGCGGCGTGGCCCAGGCGGTGCGAA > AM260479/2110873‑2111149
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
