Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A0 F0 I1 R1
|
149 |
26.7 |
2337422 |
87.5% |
2045244 |
105.0 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
AM260479 |
3,333,763 |
+G |
coding (5/615 nt) |
serB2 → |
phosphoserine phosphatase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | AM260479 | 3,333,760 | 1 | . | G | 100.0%
| 99.5
/ NA
| 26 | M1R (ATG→AGG) † | serB2 | phosphoserine phosphatase |
| Reads supporting (aligned to +/- strand): ref base . (0/0); new base G (14/12); total (14/12) |
CGGCCCCGGCGCCTGCCTGCCTGCCCGCCCGACCTCTCGCCTTAAGGTAAAATCGCCGCCCATGAATCTGGCACTCTTTGACCTCGACCACACCCTGATCCCGACCGACAGCGACCATGAAT‑GGGCCGCTTCCTGGTCCGCCTGGGCGTCGTCGACGAGGAAACCTACCGGCAGAAGAACGACGAGTTCTACGGCCACTACAAAGCCGGCACCCTCGATATCCAGGCCTTCCTGCGCTTT > AM260479/3333639‑3333878
|
cGGCCCCGGCGCCTGCCTGCCTGCCCGCCCGACCTCTCGCCTTAAGGTAAAATCGCCGCCCATGAATCTGGCACTCTTTGACCTCGACCACACCCTGATCCCGACCGACAGCGACCATGAATGGGGCCGCTTcc > 2:1147419/1‑134 (MQ=255)
cGGCCCCGGCGCCTGCCTGCCTGCCCGCCCGACCTCTCGCCTTAAGGTAAAATCGCCGCCCATGAATCTGGCACTCTTTGACCTCGACCACACCCTGATCCCGACCGACAGCGACCATGAATGGGGCCGCTTcc > 2:359810/1‑134 (MQ=255)
ggCCCCGGCGCCTGCCTGCCTGCCCGCCCGACCTCTCGCCTTAAGGTAAAATCGCCGCCCATGAATCTGGCACTCTTTGACCTCGACCACACCCTGATCCCGACCGACAGCGACCATGAATGGGGCCGCTTCCt < 1:359810/134‑1 (MQ=255)
gCCCCGGCGCCTGCCTGCCTGCCCGCCCGACCTCTCGCCTTAAGGTAAAATCGCCGCCCATGAATCTGGCACTCTTTGACCTCGACCACACCCTGATCCCGACCGACAGCGACCATGAATGGGGCCGCTTCCTg > 1:13316/1‑134 (MQ=255)
aaGGTAAAATCGCCGCCCATGAATCTGGCACTCTTTGACCTCGACCACACCCTGATCCCGACCGACAGCGACCATGAATGGGGCCGCTTCCTGGTCCGCCTGGGCGTCGTCGACGAGGAAACCTACCGGCag < 2:13316/132‑1 (MQ=255)
ccATGAATCTGGCACTCTTTGACCTCGACCACACCCTGATCCCGACCGACAGCGACCATGAAt < 2:612976/63‑1 (MQ=255)
ccATGAATCTGGCACTCTTTGACCTCGACCACACCCTGATCCCGACCGACAGCGACCATGAAt > 1:612976/1‑63 (MQ=255)
ccATGAATCTGGCACTCTTTGACCTCGACCACACCCTGATCCCGACCGACAGCGACCATGAATGGGGCCGCTTCCTGGTCCGCCTGGGCGTCGTCGACGAGTAAACCTACCGGCAGAAGAACGACGAGTTCTAc > 2:875397/1‑134 (MQ=255)
cATGAATCTGGCACTCTTTGACCTCGACCACACCCTGATCCCGACCGACAGCGACCATGAATGGGGCCGCTTCCTGGTCCGCCTGGGCGTCGTCGACGAGGAAACCTACCGGCAGAAGAACGACGAGTTCTACg > 2:312487/1‑134 (MQ=255)
aaTCTGGCACTCTTTGACCTCGACCACACCCTGATCCCGACCGACAGCGACCATGAATGGGGCCGCTTCCTGGTCCGCCTGGGCGTCGTCGACGAGGAAACCTACCGGCAGAAGAACGACGAGTTCTACGGCCa < 1:1147419/134‑1 (MQ=255)
gACCTCGACCACACCCTGATCCCGACCGACAGCGACCATGAATGGGGCCGCTTCCTGGTCCGCCTGGGCGTCGTCGACGAGGAAACCTACCGGCAGAAGAACGACGAGTTCTACGGCCACTACAAAGCCGGCa < 1:312487/133‑1 (MQ=255)
aCCACACCCTGATCCCGACCGACAGCGACCATGAATGGGGCCGCTTCCTGGTCCGCCTGGGCGTCGTCGACGAGTAAACCTACCGGCAGAAGAACGACGAGTTCTACGGCCACTACAAAGCCGGCACCCTCGat < 1:875397/134‑1 (MQ=255)
cacaCCCTGATCCCGACCGACAGCGACCATGAATGGGGCCGCTTCCTGGTCCGCCTGGGCGTCGTCGACGAGGAAACCTACCGGCAGAAGAACGACGAGTTCTa < 2:947956/104‑1 (MQ=255)
cacaCCCTGATCCCGACCGACAGCGACCATGAATGGGGCCGCTTCCTGGTCCGCCTGGGCGTCGTCGACGAGGAAACCTACCGGCAGAAGAACGACGAGTTCTa > 1:947956/1‑104 (MQ=255)
cacaCCCTGATCCCGACCGACAGCGACCATGAATGGGGCCGCTTCCTGGTCCGCCTGGGCGTCGTCGACGAGGAAACCTACCGGCAGAAGAACGACGAGTTCTACGGCCACTACAAAGCCGGCAc < 2:1018298/125‑1 (MQ=255)
cacaCCCTGATCCCGACCGACAGCGACCATGAATGGGGCCGCTTCCTGGTCCGCCTGGGCGTCGTCGACGAGGAAACCTACCGGCAGAAGAACGACGAGTTCTACGGCCACTACAAAGCCGGCAc > 1:1018298/1‑125 (MQ=255)
cacaCCCTGATCCCGACCGACAGCGACCATGAATGGGGCCGCTTCCTGGTCCGCCTGGGCGTCGTCGACGAGGAAACCTACCGGCAGAAGAACGACGAGTTCTACGGCCACTACAAAGCCGGCACCCTCGatat > 1:392049/1‑134 (MQ=255)
cacCCTGATCCCGACCGACAGCGACCATGAATGGGGCCGCTTcc > 2:974452/1‑44 (MQ=38)
cacCCTGATCCCGACCGACAGCGACCATGAATGGGGCCGCTTcc < 1:974452/44‑1 (MQ=38)
cgacAGCGACCATGAATGGGGCCGCTTCCTGGTCCGCCTGGGCGTCGTCGACGAGGAAACCTACCGGCAGAAGAACGACGAGTTCTACGGCCACTACAAAGCCGGCAcc < 2:36229/109‑1 (MQ=255)
cgacAGCGACCATGAATGGGGCCGCTTCCTGGTCCGCCTGGGCGTCGTCGACGAGGAAACCTACCGGCAGAAGAACGACGAGTTCTACGGCCACTACAAAGCCGGCAcc > 1:36229/1‑109 (MQ=255)
acAGCGACCATGAATGGGGCCGCTTCCTGGTCCGCCTGGGCGTCGTCGACGAGGAAACCTACCGGCAGAAGAACGACGAGTTCTACGGCCACTACAAAGcc < 2:712008/101‑1 (MQ=255)
acAGCGACCATGAATGGGGCCGCTTCCTGGTCCGCCTGGGCGTCGTCGACGAGGAAACCTACCGGCAGAAGAACGACGAGTTCTACGGCCACTACAAAGcc > 1:712008/1‑101 (MQ=255)
acAGCGACCATGAATGGGGCCGCTTCCTGGTCCGCCTGGGCGTCGTCGACGAGGAAACCTACCGGCAGAAGAACGACGAGTTCTACGGCCACTACAAAGCCGGCACCCTCGATATc < 2:1002739/116‑1 (MQ=255)
acAGCGACCATGAATGGGGCCGCTTCCTGGTCCGCCTGGGCGTCGTCGACGAGGAAACCTACCGGCAGAAGAACGACGAGTTCTACGGCCACTACAAAGCCGGCACCCTCGATATc < 1:864161/116‑1 (MQ=255)
acAGCGACCATGAATGGGGCCGCTTCCTGGTCCGCCTGGGCGTCGTCGACGAGGAAACCTACCGGCAGAAGAACGACGAGTTCTACGGCCACTACAAAGCCGGCACCCTCGATATc > 2:864161/1‑116 (MQ=255)
acAGCGACCATGAATGGGGCCGCTTCCTGGTCCGCCTGGGCGTCGTCGACGAGGAAACCTACCGGCAGAAGAACGACGAGTTCTACGGCCACTACAAAGCCGGCACCCTCGATATc > 1:1002739/1‑116 (MQ=255)
acAGCGACCATGAATGGGGCCGCTTCCTGGTCCGCCTGGGCGTCGTCGACGAGGAAACCTACCGGCAGAAGAACGACGAGTTCTACGGCCACTACAAAGCCGGCACCCTCGATATCCAGGCCTTCCTGCGCttt > 2:56078/1‑134 (MQ=255)
|
CGGCCCCGGCGCCTGCCTGCCTGCCCGCCCGACCTCTCGCCTTAAGGTAAAATCGCCGCCCATGAATCTGGCACTCTTTGACCTCGACCACACCCTGATCCCGACCGACAGCGACCATGAAT‑GGGCCGCTTCCTGGTCCGCCTGGGCGTCGTCGACGAGGAAACCTACCGGCAGAAGAACGACGAGTTCTACGGCCACTACAAAGCCGGCACCCTCGATATCCAGGCCTTCCTGCGCTTT > AM260479/3333639‑3333878
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
BRESEQ :: bam2aln output
ACCGCACCCAATGCGGCCCCGGCGCCTGCCTGCCTGCCCGCCCGACCTCTCGCCTTAAGGTAAAATCGCCGCCCATGAATCTGGCACTCTTTGACCTCGACCACA‑CCCTGATCCCGACCGACAGCGACCATGAAT‑GGGCCGCTTCCTGGTCCGCCTGGGCGTCGTCGACGAGGAAACCTACCGGCAGAAGAACGACGAGTTCTACGGCCACTACAAAGCCGGCACCCTCGATATCCAGGCCTTCCTGCGCTTTGCGCTGGCGCCGCTGGCGGCCA > AM260479/3333626‑3333900
|
ACCGCACCCAATGCGGCCCCGGCGCCTGCCTGCCTGCCCGCCCGACCTCTCGCCTTAAGGTAAAATCGCCGCCCATGAATCTGGCACTCTTTGACCTCGACCACA‑CCCTGATCCCGACCGACAGCGACCATGAATGGGGCCGCTTCCTGG > GWNJ‑0478:712:GW2002102894th:2:1101:1688:87971/1‑150 (MQ=60)
ttgatttttttttttttttcaagcagaagacggcatacgagattagcgagtgtctcgtgggctcggagatgtgtataagagacagCTTTGACCTCGACCACA‑CCCTGATCCCGACCGACAGCGACCATGAATGGGGCCGCTTCCTGGTC < GWNJ‑0478:712:GW2002102894th:2:1205:10367:20827/64‑1 (MQ=60)
tttttttttcaagcagaagacggcatacgagattagcgagtgtctcgtgggctcggagatgtgtataagagacagCTTTGACCTCGACCACA‑CCCTGATCCCGACCGACAGCGACCATGAATGGGGCCGCTTCCTGGTCCGCCTGGGCG < GWNJ‑0478:712:GW2002102894th:2:2206:18616:30390/74‑1 (MQ=60)
CGGCCCCGGCGCCTGCCTGCCTGCCCGCCCGACCTCTCGCCTTAAGGTAAAATCGCCGCCCATGAATCTGGCACTCTTTGACCTCGACCACA‑CCCTGATCCCGACCGACAGCGACCATGAATGGGGCCGCTTCCTGGTCCGCCTGGGCGT < GWNJ‑0478:712:GW2002102894th:2:1204:6734:80013/150‑1 (MQ=60)
AGGTAAAATCGCCGCCCATGAATCTGGCACTCTTTGACCTCGACCACA‑CCCTGATCCCGACCGACAGCGACCATGAATGGGGCCGCTTCCTGGctgtctcttatacacatctccgagcccacgagacactcgctaatctcgtatgccgtc > GWNJ‑0478:712:GW2002102894th:2:2102:12802:46692/1‑93 (MQ=60)
GAATCTGGCACTCTTTGACCTCGACCACA‑CCCTGATCCCGACCGACAGCGACCATGAATGGGGCCGCTTCCTGGTCCGCCTGGGCGTCGTCGACGAGGAAACCTACCGGCAGAAGAACGACGAGTTCTACGGCCACTACAAAGCCGGCAC < GWNJ‑0478:712:GW2002102894th:2:2216:12365:8277/150‑1 (MQ=60)
CTCTTTGACCTCGACCACA‑CCCTGATCCCGACCGACAGCGACCATGAATGGGGCCGCTTCCTGGTCCGCCTGGGCGTCGTCGACGAGGAAACCTACCGGCAGAAGAACGACGAGTTCTACGGCCACTACAAAGCCtgtctcttatacac > GWNJ‑0478:712:GW2002102894th:2:2205:6099:83551/1‑135 (MQ=60)
CTCTTTGACCTCGACCACA‑CCCTGATCCCGACCGACAGCGACCATGAATGGGGCCGCTTCCTGGTCCGCCTGGGCGTCGTCGACGAGGAAACCTACCGGCAGAAGAACGACGAGTTCTACGGCCACTACAAAGCCGGCACCCTCGATATC > GWNJ‑0478:712:GW2002102894th:2:1206:14183:72244/1‑150 (MQ=60)
CTCTTTGACCTCGACCACA‑CCCTGATCCCGACCGACAGCGACCATGAATGGGGCCGCTTCCTGGTCCGCCTGGGCGTCGTCGACGAGGAAACCTACCGGCAGAAGAACGACGAGTTCTACGGCCACTACAAAGCCGGCACCCTCGATATC > GWNJ‑0478:712:GW2002102894th:2:2208:19130:79808/1‑150 (MQ=60)
TGACCTCGACCACA‑CCCTGATCCCGACCGACAGCGACCATGAATGGGGCCGCTTCCTGGTCCGCCTGGGCGTCGTCGACGAGGAAACCTACCGGCAGAAGAACGACGAGTTCTACGGCCACTACAAAGCCGGCACCCTCGATATCCAGG < GWNJ‑0478:712:GW2002102894th:2:1203:5221:80535/149‑1 (MQ=60)
GACCACA‑CCCTGATCCCGACCGACAGCGACCATGAATGGGGCCGCTTCCTGGTCCGCCTGGGCGTCGTCGACGAGTAAACCTACCGGCAGAAGAACGACGAGTTCTACGGCCACTACAAAGCCGGCACCCTCGATATCCAGGCCTTCCTG < GWNJ‑0478:712:GW2002102894th:2:2201:12662:38839/150‑1 (MQ=60)
ACAGCCCTGATCCCGACCGACAGCGACCATGAATGGGGCCGCTTCCTGGTCCGCCTGGGCGTCGTCGACGAGGAAACCTACCGGCAGAAGAACGACGAGTTCTACGGCCACTACAAAGCCGGCACCCTCGATATCCAGGCCTTCCTGCGC < GWNJ‑0478:712:GW2002102894th:2:2116:2712:74033/150‑1 (MQ=60)
CA‑CCCTGATCCCGACCGACAGCGACCATGAATGGGGCCGCTTCCTGGTCCGCCTGGGCGTCGTCGACGAGGAAACCTACCGGCAGAAGAACGACGAGTTCTACGGCCACTACAAAGCCGGCACCCTCGATATCCAGGCCctgtctcttat > GWNJ‑0478:712:GW2002102894th:2:1105:9279:31805/1‑139 (MQ=60)
CCCTGATCCCGACCGACAGCGACCATGAATGGGGCCGCTTCCTGGTCCGCCTGGGCGTCtgtctcttatacacatctccgagcccacgagacactcgctaatctcgtatgccgtcttctgcttgaaaaaaaaaaaaagaaacaaaacc > GWNJ‑0478:712:GW2002102894th:2:1106:14659:23130/1‑59 (MQ=60)
CCCTGATCCCGACCGACAGCGACCATGAATGGGGCCGCTTCCTGGTCCGCCTGGGCGTCGTCGACGAGGAAACCTACCGGCAGAAGAACGACGAGTTCTACGGCCACTACAAAGCCGGCACCCTCGATATCCtgtctcttatacacatct > GWNJ‑0478:712:GW2002102894th:2:2109:7595:23598/1‑132 (MQ=60)
CCCTGATCCCGACCGACAGCGACCATGAATGGGGCCGCTTCCTGGTCCGCCTGGGCGTCGTCGACGAGGAAACCTACCGGCAGAAGAACGACGAGTTCTACGGCCACTACAAAGCCGGCACCCTCGATATCCAGGCCTTCCTGCGCctgt > GWNJ‑0478:712:GW2002102894th:2:2207:11511:92734/1‑146 (MQ=60)
CGACCATGAATGGGGCCGCTTCCTGGTCCGCCTGGGCGTCGTCGACGAGGAAACCTACCGGCAGAAGAACGACGAGTTCTACGGCCACTACAAAGCCGGCACCCTCGATATCCAGGCCTTCCTGCGCctgtctcttatacacatctccg > GWNJ‑0478:712:GW2002102894th:2:2116:19890:68090/1‑127 (MQ=60)
ACCATGAATGGGGCCGCTTCCTGGTCCGCCTGGGCGTCGTCGACGAGGAAACCTACCGGCAGAAGAACGACGAGTTCTACGGCCACTACAAAGCCGGCACCCTCGATATCCtgtctcttatacacatctccgagcccacgagacactcgc > GWNJ‑0478:712:GW2002102894th:2:1115:10774:27035/1‑111 (MQ=60)
ACCATGAATGGGGCCGCTTCCTGGTCCGCCTGGGCGTCGTCGACGAGGAAACCTACCGGCAGAAGAACGACGAGTTCTACGGCCACTACAAAGCCGGCACCCTCGATATCCAGGCCTTCCTGCGCTTTGCGCTGGCGCCGCTGGCGGCC > GWNJ‑0478:712:GW2002102894th:2:1211:6429:81872/1‑149 (MQ=60)
|
ACCGCACCCAATGCGGCCCCGGCGCCTGCCTGCCTGCCCGCCCGACCTCTCGCCTTAAGGTAAAATCGCCGCCCATGAATCTGGCACTCTTTGACCTCGACCACA‑CCCTGATCCCGACCGACAGCGACCATGAAT‑GGGCCGCTTCCTGGTCCGCCTGGGCGTCGTCGACGAGGAAACCTACCGGCAGAAGAACGACGAGTTCTACGGCCACTACAAAGCCGGCACCCTCGATATCCAGGCCTTCCTGCGCTTTGCGCTGGCGCCGCTGGCGGCCA > AM260479/3333626‑3333900
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
