Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A0 F0 I1 R1
|
149 |
26.7 |
2337422 |
87.5% |
2045244 |
105.0 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
AM260479 |
3,529,526 |
+G |
intergenic (‑40/‑264) |
secA ← / → h16_A3265 |
preprotein translocase subunit SecA/Hypothetical protein |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | AM260479 | 3,529,524 | 1 | . | G | 100.0%
| 59.6
/ NA
| 17 | intergenic (‑38/‑266) | secA/h16_A3265 | preprotein translocase subunit SecA/Hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base . (0/0); new base G (10/7); total (10/7) |
CAGCGCATTGATCTGCGCCACCGTGCGGCGATATTGTTTGATCAGCCGCTCATTGCGGCTGCCGAAGACTTTCTTGAGAAGGCCGGTGATCATCGATTGCTTTCACCTGCGCGGAACCGGGCGGCACCCCA‑GGATGCGGGCTGCTCCGGCGGCCGGGCGAGGAATGGATAGATTGGCGCAAAACGCCGAGTTTATCATGTGCCGGGGTCACGG‑CGGAACG > AM260479/3529394‑3529613
|
cAGCGCATTGATCTGCGCCACCGTGCGGCGATATTGTTTGATCAGCCGCTCATTGCGGCTGCCGAAGACTTTCTTGAGAAGGCCGGTGATCATCGATTGCTTTCACCTGCGCGGAACCGGGCGGCACCCCA‑g < 1:722915/132‑1 (MQ=255)
gcgATATTGTTTGATCAGCCGCTCATTGCGGCTGCCGAAGACTTTCTTGAGAAGGCCGGTGATCATCGATTGCTTTCACCTGCGCGGAACCGGGCGGCACCCCAGGGATGCGGGCTGCTCCGGCGGCCGGGc > 1:962260/1‑132 (MQ=255)
gcgATATTGTTTGATCAGCCGCTCATTGCGGCTGCCGAAGACTTTCTTGAGAAGGCCGGTGATCATCGATTGCTTTCACCTGCGCGGAACCGGGCGGCACCCCA‑gggatg > 1:931634/1‑106 (MQ=255)
gcgATATTGTTTGATCAGCCGCTCATTGCGGCTGCCGAAGACTTTCTTGAGAAGGCCGGTGATCATCGATTGCTTTCACCTGCGCGGAACCGGGCGGCACCCCA‑gggatg < 2:931634/110‑5 (MQ=255)
gCCGCTCATTGCGGCTGCCGAAGACTTTCTTGAGAAGGCCGGTGATCATCGATTGCTTTCACCTGCGCGGAACCGGGCGGCACCCCAGGGATGCGGGCTGCTCCGGCGGCCGGGCg > 1:426906/1‑116 (MQ=255)
gCCGCTCATTGCGGCTGCCGAAGACTTTCTTGAGAAGGCCGGTGATCATCGATTGCTTTCACCTGCGCGGAACCGGGCGGCACCCCAGGGATGCGGGCTGCTCCGGCGGCCGGGCg < 2:426906/116‑1 (MQ=255)
tGCCGAAGACTTTCTTGAGAAGGCCGGTGATCATCGATTGCTTTCACCTGCGCGGAACCGGGCGGCACCCCAGGGATGCGGGCTGCTCCGGCGGCCGGGCGAGGAATGGATAGATTGGCGCAAAACGCCGAGtt > 1:505574/1‑134 (MQ=255)
tGCCGAAGACTTTCTTGAGAAGGCCGGTGATCATCGATTGCTTTCACCTGCGCGGAACCGGGCGGCACCCCAGGGATGCGGGCTGCTCCGGCGGCCGGGCGAGGAATGGATAGATTGGCGCAAAACGCCGAGtt > 1:760077/1‑134 (MQ=255)
cGAAGACTTTCTTGAGAAGGCCGGTGATCATCGATTGCTTTCACCTGCGCGGAACCGGGCGGCACCCCAGGGATGCGGGCTGCTCCGGCGGCCGGGCg < 1:113717/98‑1 (MQ=255)
cGAAGACTTTCTTGAGAAGGCCGGTGATCATCGATTGCTTTCACCTGCGCGGAACCGGGCGGCACCCCAGGGATGCGGGCTGCTCCGGCGGCCGGGCg > 2:113717/1‑98 (MQ=255)
aGACTTTCTTGAGAAGGCCGGTGATCATCGATTGCTTTCACCTGCGCGGAACCGGGCGGCACCCCAGGGATGCGGGCTGCTCCGGCGGCCGGGCGAGGAATGGATAGATTGGCGCAAAACGCCGAGTTTATCAt > 2:133866/1‑134 (MQ=255)
atcatcGATTGCTTTCACCTGCGCGGAACCGGGCGGCACCCCAGGGATGCGGGCTGCTCCGGCGGCCGGGCGAGGAATGGATAGATTGGCGCAAAACGCCGAGTTTATCATGTGCCGGGGTCACGGCCGGAAcg < 1:133866/134‑1 (MQ=255)
aTTGCTTTCACCTGCGCGGAACCGGGCGGCACCCCAGGGATGCGGGCTGCTCCGGCGGCCGGGCGAGGAATGGATAGATTGGCGCaaa > 1:700158/1‑88 (MQ=255)
aTTGCTTTCACCTGCGCGGAACCGGGCGGCACCCCAGGGATGCGGGCTGCTCCGGCGGCCGGGCGAGGAATGGATAGATTGGCGCaaa < 2:700158/88‑1 (MQ=255)
gcgcGGAACCGGGCGGCACCCCAGGGATGCGGGCTGCTCCGGCGGcc < 1:819889/47‑1 (MQ=38)
gcgcGGAACCGGGCGGCACCCCAGGGATGCGGGCTGCTCCGGCGGcc > 2:819889/1‑47 (MQ=38)
gAACCGGGCGGCACCCCAGGGATGCGGGCTGCTCCGGCGGCCGGGCg < 1:602256/47‑1 (MQ=38)
gAACCGGGCGGCACCCCAGGGATGCGGGCTGCTCCGGCGGCCGGGCg > 2:602256/1‑47 (MQ=38)
gAACCGGGCGGCACCCCAGGGATGCGGGCTGCTCCGGCGGCCGGGCGAGGACTGGATAGATTGGCGCaaa > 1:426264/1‑70 (MQ=255)
gAACCGGGCGGCACCCCAGGGATGCGGGCTGCTCCGGCGGCCGGGCGAGGAATGGATAGATTGGCGCaaa < 2:426264/70‑1 (MQ=255)
|
CAGCGCATTGATCTGCGCCACCGTGCGGCGATATTGTTTGATCAGCCGCTCATTGCGGCTGCCGAAGACTTTCTTGAGAAGGCCGGTGATCATCGATTGCTTTCACCTGCGCGGAACCGGGCGGCACCCCA‑GGATGCGGGCTGCTCCGGCGGCCGGGCGAGGAATGGATAGATTGGCGCAAAACGCCGAGTTTATCATGTGCCGGGGTCACGG‑CGGAACG > AM260479/3529394‑3529613
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
BRESEQ :: bam2aln output
CGAACTTCGGCTCCAGCGCATTGATCTGCGCCACCGTGCGGCGATATTGTTTGATCAGCCGCTCATTGCGGCTGCCGAAGACTTTCTTGAGAAGGCCGGTGATCATCGATTGCTTTCACCTGCGCGGAACCGGGCGGCACCCCA‑GGATGCGGGCTGCTCCGGCGGCCGGGCGAGGAATGGATAGATTGGCGCAAAACGCCGAGTTTATCATGTGCCGGGGTCACGG‑CGGAACGCGCCTGGCCCCGTCCCGCAAGGGCAGGCAAGCCTGCCGGATGTCGGCCACGCGTGTGGT > AM260479/3529381‑3529672
|
CGAACTTCGGCTCCAGCGCATTGATCTGCGCCACCGTGCGGCGATATTGTTTGATCAGCCGCTCATTGCGGCTGCCGAAGACTTTCTTGAGAAGGCCGGTGATCATCGATTGCTTTCACCTGCGCGGAACCGGGCGGCACCCCA‑GGga < GWNJ‑0478:712:GW2002102894th:2:2213:7414:42463/148‑3 (MQ=60)
CCAGCGCATTGATCTGCGCCACCGTGCGGCGATATTGTTTGATCAGCCGCTCATTGCGGCTGCCGAAGACTTTCTTGAGAAGGCCGGTGATCATCGATTGCTTTCACCTGCGCGGAACCGGGCGGCACCCCAGGGATGCGGGCTGCTC < GWNJ‑0478:712:GW2002102894th:2:2109:9515:82900/148‑1 (MQ=60)
CTGCGCCACCGTGCGGCGATATTGTTTGATCAGCCGCTCATTGCGGCTGCCGAAGACTTTCTTGAGAAGGCCGGTGATCATCGATTGCTTTCACCTGCGCGGAACCGGGCGGCACCCCAGGGATGCGGGCTGCTCCGGCGctgtctctt > GWNJ‑0478:712:GW2002102894th:2:2204:16552:86403/1‑140 (MQ=60)
CTGCGCCACCGTGCGGCGATATTGTTTGATCAGCCGCTCATTGCGGCTGCCGAAGACTTTCTTGAGAAGGCCGGTGATCATCGATTGCTTTCACCTGCGCGGAACCGGGCGGCACCCCAGGGATGCGGGCTGCTCCGGCGGCCGGGCG > GWNJ‑0478:712:GW2002102894th:2:2209:12513:63500/1‑148 (MQ=60)
ttttttaaagcagaagacggcatacgagattagcgagtgtctcgtgggctcggagatgtgtataagagacagCGATTGCTTTCACCTGCGCGGAACCGGGCGGCACCCCAGGGATGCGGGCTGCTCCGGCGGCCGGGCGAGGAATGGAT < GWNJ‑0478:712:GW2002102894th:2:2115:2684:68404/77‑1 (MQ=60)
cggagatgtgtataagagacagGCTCATTGCGGCTGCCGAAGACTTTCTTGAGAAGGCCGGTGATCATCGATTGCTTTCACCTGCGCGGAACCGGGCGGCACCCCAGGGATGCGGGCTGCTCCGGCGGCCGGGCGAGGAATGGATAGATT < GWNJ‑0478:712:GW2002102894th:2:1108:17420:7661/128‑1 (MQ=60)
ttttttcaagcagaagacggcatacgagattagcgagtgtctcgtgggctcggagatgtgtataagagacagGCTTTCACCTGCGCGGAACCGGGCGGCACCCCAGGGATGCGGGCTGCTCCGGCGGCCGGGCGAGGAATGGATAGATT < GWNJ‑0478:712:GW2002102894th:2:2103:4491:75657/77‑1 (MQ=60)
GATATTGTTTGATCAGCCGCTCATTGCGGCTGCCGAAGACTTTCTTGAGAAGGCCGGTGATCATCGATTGCTTTCACCTGCGCGGAACCGGGCGGCACCCCAGGGATGCGGGCTGCTCCGGCGGCCGGGCGAGGAATGGATAGATTctgt > GWNJ‑0478:712:GW2002102894th:2:1209:17617:70138/1‑146 (MQ=60)
GCCGCTCATTGCGGCTGCCGAAGACTTTCTTGAGAAGGCCGGTGATCATCGATTGCTTTCACCTGCGCGGAACCGGGCGGCACCCCAGGGATGCGGGCTGCTCCGGCGGCCGGGCGAGGAATGGATAGATTGGCGCAAAACGCCGAGTTT > GWNJ‑0478:712:GW2002102894th:2:1212:16325:76854/1‑150 (MQ=60)
GCCGCTCATTGCGGCTGCCGAAGACTTTCTTGAGAAGGCCGGTGATCATCGATTGCTTTCACCTGCGCGGAACCGGGCGGCACCCCAGGGATGCGGGCTGCTCCGGCGGCCGGGCGAGGAATGGATAGATTGGCGCAAAACGCCGAGTTT > GWNJ‑0478:712:GW2002102894th:2:2110:19875:74511/1‑150 (MQ=60)
aagcagaagacggcatacgagattagcgagtgtctcgtgggctcggagatgtgtataagagacaGGAACCGGGCGGCACCCCAGGGATGCGGGCTGCTCCGGCGGCCGGGCGAGGAATGGATAGATTGGCGCAAAACGCCGAGTTTATCA < GWNJ‑0478:712:GW2002102894th:2:1205:10154:92442/86‑1 (MQ=60)
GGCCGGTGATCATCGATTGCTTTCACCTGCGCGGAACCGGGCGGCACCCCAGGGATGCGGGCTGCTCCGGCGGCCGGGCGAGGAATGGATAGATTGGCGCAAAACGCCGAGTTTATCActgtctcttatacacatctccgagcccacg > GWNJ‑0478:712:GW2002102894th:2:2108:14020:58707/1‑118 (MQ=60)
GATCATCGATTGCTTTCACCTGCGCGGAACCGGGCGGCACCCCAGGGATGCGGGCTGCTCCGGCGGCCGGGCGAGGAATGGATAGATTGGCGCAAAACGCCGAGTTTATCATGTGCCGGGGTCACGGCCGGAACGCGCCTGGCCCCGTCC < GWNJ‑0478:712:GW2002102894th:2:1109:16946:21716/150‑1 (MQ=60)
cgtgggctcggagatgtgtataagagacagccagGGATGCGGGCTGCTCCGGCGGCCGGGCGAGGAATGGATAGATTGGCGCAAAACGCCGAGTTTATCATGTGCCGGGGTCACGGCCGGAACGCGCCTGGCCCCGTCCCGCAAGGG < GWNJ‑0478:712:GW2002102894th:2:1206:1822:78883/113‑1 (MQ=60)
GCTTTCACCTGCGCGGAACCGGGCGGCACCCCAGGGATGCGGGCTGCTCCGGCGGCCGGGCGAGGACTGGATAGATTGGCGCAAAACGCCGAGTTTATCActgtctcttatacacatctccgagcccacgagacactcgctaatctcgt > GWNJ‑0478:712:GW2002102894th:2:1209:6784:66721/1‑100 (MQ=60)
GCACCCCAGGGATGCGGGCTGCTCCGGCGGCCGGGCGAGGAATGGATAGATTGGCGCAAAACGCCGAGTTTATCATGTGCCGGGGTCACGGCCGGAACGCGCCTGGCCCCGTCCCGCAAGGGCAGGCAAGCCTGCCGGATGTCGGCCACG > GWNJ‑0478:712:GW2002102894th:2:2101:8208:36513/1‑150 (MQ=60)
CCCCAGGGATGCGGGCTGCTCCGGCGGCCGGGCGAGGAATGGATAGATTGGCGCAAAACGCCGAGTTTATCATGTGCCGGGGTCACGGCCGGAACGCGCCTGGCCCCGTCCCGCAAGGGCAGGCAAGCCTGCCGGATGTCGGCCACGCGT > GWNJ‑0478:712:GW2002102894th:2:1115:10316:16418/1‑150 (MQ=60)
GGATGCGGGCTGCTCCGGCGGCCGGGCGAGGAATGGATAGATTGGCGCAAAACGCCGAGTTTATCATGTGCCtgtctcttatacacatctccgagcccacgagacactcgctaatctcgtatgccgtcttctgcttgaaaaaaaaaaaa > GWNJ‑0478:712:GW2002102894th:2:2204:5053:97636/1‑72 (MQ=60)
GGATGCGGGCTGCTCCGGCGGCCGGGCGAGGAATGGATAGATTGGCGCAAAACGCCGAGTTTATCATGTGCCtgtctcttatacacatctccgagcccacgagacactcgctaatctcgtatgccgtcttctgcttgaaaaaaaaaaaa > GWNJ‑0478:712:GW2002102894th:2:1110:6556:64933/1‑72 (MQ=60)
|
CGAACTTCGGCTCCAGCGCATTGATCTGCGCCACCGTGCGGCGATATTGTTTGATCAGCCGCTCATTGCGGCTGCCGAAGACTTTCTTGAGAAGGCCGGTGATCATCGATTGCTTTCACCTGCGCGGAACCGGGCGGCACCCCA‑GGATGCGGGCTGCTCCGGCGGCCGGGCGAGGAATGGATAGATTGGCGCAAAACGCCGAGTTTATCATGTGCCGGGGTCACGG‑CGGAACGCGCCTGGCCCCGTCCCGCAAGGGCAGGCAAGCCTGCCGGATGTCGGCCACGCGTGTGGT > AM260479/3529381‑3529672
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
