Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A0 F0 I1 R1
|
149 |
26.7 |
2337422 |
87.5% |
2045244 |
105.0 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
AM260480 |
157,096 |
+G |
intergenic (‑26/+41) |
h16_B0136 ← / ← h16_B0137 |
cysteine desulfurase/conserved hypothetical membrane protein |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | AM260480 | 157,094 | 1 | . | G | 100.0%
| 97.1
/ NA
| 26 | intergenic (‑24/+43) | h16_B0136/h16_B0137 | cysteine desulfurase/conserved hypothetical membrane protein |
| Reads supporting (aligned to +/- strand): ref base . (0/0); new base G (12/14); total (12/14) |
TCGGGCGGCAACGAGGCCGCGGCGGTGTCGAGATAGATGGTGGAGGCCGTCAGCGGGGTGTCGGCGCGGATCGCCTGGATGGCTTGGATATCCATGGAACGTCTCGGGTAATGACAGGC‑GGACGCGGCGACCGCGCCCCGAGGTAAGACTCAGTTGCGCACTTACTTGCGCAGCTTGGTGACGGAGCCGAAGAAGCTGAACAGGGCGTCGCCGGCAATCACGCCGCCGGCGAAG > AM260480/156976‑157209
|
tCGGGCGGCAACGAGGCCGCGGCGGTGTCGAGATAGATGGTGGAGGCCGTCAGCGGGGTGTCGGCGCGGATCGCCTGGATGGCTTGGATATCCATGGAACGTCTCGGGTAATGACAGGCGGGACGCGGCGACcg > 2:227684/1‑134 (MQ=255)
tCGGGCGGCAACGAGGCCGCGGCGGTGTCGAGATAGATGGTGGAGGCCGTCAGCGGGGTGTCGGCGCGGATCGCCTGGATGGCTTGGATATCCATGGAACGTCTCGGGTAATGACAGGCGGGACGCGGCGACcg > 2:638640/1‑134 (MQ=255)
tCGGGCGGCAACGAGGCCGCGGCGGTGTCGAGATAGATGGTGGAGGCCGTCAGCGGGGTGTCGGCGCGGATCGCCTGGATGGCTTGGATATCCATGGAACGTCTCGGGTAATGACAGGCGGGACGCGGCGACcg > 1:882177/1‑134 (MQ=255)
gggCGGCAACGAGGCCGCGGCGGTGTCGAGATAGATGGTGGAGGCCGTCAGCGGGGTGTCGGCGCGGATCGCCTGGATGGCTTGGATATCCATGGAACGTCTCGGGTAATGACAGGCGGGACGCGGCGACcgcg > 2:695209/1‑134 (MQ=255)
gcggcAACGAGGCCGCGGCGGTGTCGAGATAGATGGTGGAGGCCGTCAGCGGGGTGTCGGCGCGGATCGCCTGGATGGCTTGGATATCCATGGAACGTCTCGGGTAATGACAGGCGGGACGCGGCGACCGCGcc < 2:803746/134‑1 (MQ=255)
cggcAACGAGGCCGCGGCGGTGTCGAGATAGATGGTGGAGGCCGTCAGCGGGGTGTCGGCGCGGATCGCCTGGATGGCTTGGATATCCATGGAACGTCTCGGGTAATGACAGGCGGGACGCGGCGACCGCGccc < 2:1073566/134‑1 (MQ=255)
ggcAACGAGGCCGCGGCGGTGTCGAGATAGATGGTGGAGGCCGTCAGCGGGGTGTCGGCGCGGATCGCCTGGATGGCTTGGATATCCATGGAACGTCTCGGGTAATGACAGGCGGGACGCGGCGACCGCGcccc < 1:1048912/134‑1 (MQ=255)
gcggTGTCGAGATAGATGGTGGAGGCCGTCAGCGGGGTGTCGGCGCGGATCGCCTGGATGGCTTGGATATCCATGGAACGTCTCGGGTAATGACAGGCGGGACGCGGCGACCGCGCCCCGAGGTAAGACTCAGt < 1:695209/134‑1 (MQ=255)
ggTGTCGAGATAGATGGTGGAGGCCGTCAGCGGGGTGTCGGCGCGGATCGCCTGGATGGCTTGGATATCCATGGAACGTCTCGGGTAATGACAGGCGGGACGCGGCGACCGCGCCCCGAGGTAAGACTCAGTTg < 1:227684/134‑1 (MQ=255)
gatagatGGTGGAGGCCGTCAGCGGGGTGTCGGCGCGGATCGCCTGGATGGCTTGGATATCCATGGAACGTCTCGGGTAATGACAGGCGGGACGCGGCGACCGCGCCCCGAGGTAAGACTCAGTTGCGCactta < 2:568833/134‑1 (MQ=255)
tagatGGTGGAGGCCGTCAGCGGGGTGTCGGCGCGGATCGCCTGGATGGCTTGGATATCCATGGAACGTCTCGGGTAATGACAGGCGGGACGCGGCGACCGCGCCCCGAGGTAAGACTCAGTTGCGCacttact < 1:638640/134‑1 (MQ=255)
tCAGCGGGGTGTCGGCGCGGATCGCCTGGATGGCTTGGATATCCATGGAACGTCTCGGGTAATGACAGGCGGGACGCGGCGACCGCGCCCCGAGGTAAGACTCAGTTGCGCACTTACTTGCGCAGCTTGGTGAc > 1:551207/1‑134 (MQ=255)
aGCGGGGTGTCGGCGCGGATCGCCTGGATGGCTTGGATATCCATGGAACGTCTCGGGTAATGACAGGCGGGACGCGGCGACCGCGCCCCGAGGTAAGACTc < 1:63895/101‑1 (MQ=255)
aGCGGGGTGTCGGCGCGGATCGCCTGGATGGCTTGGATATCCATGGAACGTCTCGGGTAATGACAGGCGGGACGCGGCGACCGCGCCCCGAGGTAAGACTc > 2:63895/1‑101 (MQ=255)
cGGGGTGTCGGCGCGGATCGCCTGGATGGCTTGGATATCCATGGAACGTCTCGGGTAATGACAGGCGGGACGCGGCGACcgc < 1:465754/82‑1 (MQ=255)
cGGGGTGTCGGCGCGGATCGCCTGGATGGCTTGGATATCCATGGAACGTCTCGGGTAATGACAGGCGGGACGCGGCGACcgc > 2:465754/1‑82 (MQ=255)
ggggTGTCGGCGCGGATCGCCTGGATGGCTTGGATATCCATGGAACGTCTCGGGTAATGACAGGCGGGACGCGGCGACCGCGCCCCGAGGTAAGACTCAGTTGCGCACTTACTTGCGCAGCTTg < 1:1071738/124‑1 (MQ=255)
ggggTGTCGGCGCGGATCGCCTGGATGGCTTGGATATCCATGGAACGTCTCGGGTAATGACAGGCGGGACGCGGCGACCGCGCCCCGAGGTAAGACTCAGTTGCGCACTTACTTGCGCAGCTTg > 2:1071738/1‑124 (MQ=255)
gATATCCATGGAACGTCTCGGGTAATGACAGGCGGGACGCGGCGACCGCGCCCCGAGGTAAGACTCAGTTGCGCACTTACTTGCGCAGCTTGGTGACGGAGCCGAAGAAGc < 1:919020/111‑1 (MQ=255)
gATATCCATGGAACGTCTCGGGTAATGACAGGCGGGACGCGGCGACCGCGCCCCGAGGTAAGACTCAGTTGCGCACTTACTTGCGCAGCTTGGTGACGGAGCCGAAGAAGc > 2:919020/1‑111 (MQ=255)
ggAACGTCTCGGGTAATGACAGGCGGGACGCGGCGACcgc < 2:741/40‑1 (MQ=38)
ggAACGTCTCGGGTAATGACAGGCGGGACGCGGCGACcgc > 1:741/1‑40 (MQ=255)
gggTAATGACAGGCGGGACGCGGCGACCGCGCCCCGAGGTAAGACTCAGTTGCGCACTTACTTGCGCAGCTTGGTGACGGAGCCGAAGAAGCTGAACAGGGCGTCGCCGGCAATCACGCCGCCGGCGAAg > 1:900822/1‑130 (MQ=255)
gggTAATGACAGGCGGGACGCGGCGACCGCGCCCCGAGGTAAGACTCAGTTGCGCACTTACTTGCGCAGCTTGGTGACGGAGCCGAAGAAGCTGAACAGGGCGTCGCCGGCAATCACGCCGCCGGCGAAg < 2:900822/130‑1 (MQ=255)
ggTAATGACAGGCGGGACGCGGCGACCGCGCCCCGAGGTAAGACTCAGTTg < 1:834019/51‑1 (MQ=38)
ggTAATGACAGGCGGGACGCGGCGACCGCGCCCCGAGGTAAGACTCAGTTg > 2:834019/1‑51 (MQ=38)
|
TCGGGCGGCAACGAGGCCGCGGCGGTGTCGAGATAGATGGTGGAGGCCGTCAGCGGGGTGTCGGCGCGGATCGCCTGGATGGCTTGGATATCCATGGAACGTCTCGGGTAATGACAGGC‑GGACGCGGCGACCGCGCCCCGAGGTAAGACTCAGTTGCGCACTTACTTGCGCAGCTTGGTGACGGAGCCGAAGAAGCTGAACAGGGCGTCGCCGGCAATCACGCCGCCGGCGAAG > AM260480/156976‑157209
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
BRESEQ :: bam2aln output
GCTGCGCACGGTATCGAGCACCGCGTCGGGCGGCAACGAGGCCGCGGCGGTGTCGAGATAGATGGTGGAGGCCGTCAGCGGGGTGTCGGCGCGGATCGCCTGGATGGCTTGGATATCCATGGAACGTCTCGGGTAATGACAGGC‑GGACGCGGCGACCGCGCCCCGAGGTAAGACTCAGTTGCGCACTTACTTGCGCAGCTTGGTGACGGAGCCGAAGAAGCTGAACAGGGCGTCGCCGGCAATCACGCCGCCGGCGAAGGCGCTCATCTCGGCCACGTGCTTGTC > AM260480/156951‑157235
|
cggcatacgagattagcgagtgtctcgtgggctcggagatgtgtataagagacaGAGATAGATGGTGGAGGCCGTCAGCGGGGTGTCGGCGCGGATCGCCTGGATGGCTTGGATATCCATGGAACGTCTCGGGTAATGACAGGCGGGACG < GWNJ‑0478:712:GW2002102894th:2:1116:12125:62775/96‑1 (MQ=60)
cggcatacgagattagcgagtgtctcgtgggctcggagatgtgtataagagacaGAGATAGATGGTGGAGGCCGTCAGCGGGGTGTCGGCGCGGATCGCCTGGATGGCTTGGATATCCATGGAACGTCTCGGGTAATGACAGGCGGGACG < GWNJ‑0478:712:GW2002102894th:2:2205:6712:57149/96‑1 (MQ=60)
acgagattagcgagtgtctcgtgggctcggagatgtgtataagagacagGATAGATGGTGGAGGCCGTCAGCGGGGTGTCGGCGCGGATCGCCTGGATGGCTTGGATATCCATGGAACGTCTCGGGTAATGACAGGCGGGACGCGGCGAC < GWNJ‑0478:712:GW2002102894th:2:2213:12269:33546/101‑1 (MQ=60)
GTATCGAGCACCGCGTCGGGCGGCAACGAGGCCGCGGCGGTGTCGAGATAGATGGTGGAGGCCGTCAGCGGGGTGTCGGCGCGGATCGCCTGGATGGCTTGGATATCCATGGAACGTCTCGGGTAATGACAGGCGGGACGCGGCGACCGC > GWNJ‑0478:712:GW2002102894th:2:2201:10591:82485/1‑150 (MQ=60)
gagtgtctcgtgggctcggagatgtgtataagagacagGGTGGAGGCCGTCAGCGGGGTGTCGGCGCGGATCGCCTGGATGGCTTGGATATCCATGGAACGTCTCGGGTAATGACAGGCGGGACGCGGCGACCGCGCCCCGAGGTAAGAC < GWNJ‑0478:712:GW2002102894th:2:1210:8734:76735/112‑1 (MQ=60)
CGGCAACGAGGCCGCGGCGGTGTCGAGATAGATGGTGGAGGCCGTCAGCGGGGTGTCGGCGCGGATCGCCTGGATGGCTTGGATATCCATGGAACGTCTCGGGTAATGACAGGCGGGACGCGGCGACCGCGCCCCGAGGTAAGACTCAGT < GWNJ‑0478:712:GW2002102894th:2:2210:12088:40619/150‑1 (MQ=60)
agatgtgtataagagacAGATGGTGGAGGCCGTCAGCGGGGTGTCGGCGCGGATCGCCTGGATGGCTTGGATATCCATGGAACGTCTCGGGTAATGACAGGCGGGACGCGGCGACCGCGCCCCGAGGTAAGACTCAGTTGCGCACTTACT < GWNJ‑0478:712:GW2002102894th:2:1104:8244:9420/133‑1 (MQ=60)
GGCGGTGTCGAGATAGATGGTGGAGGCCGTCAGCGGGGTGTCGGCGCGGATCGCCTGGATGGCTTGGATATCCATGGAACGTCTCGGGTAATGACAGGCGGGACGCGGCGACCGCGCCCCGAGGTAAGACTCAGTTGCGCACTTACTTGC < GWNJ‑0478:712:GW2002102894th:2:2108:19687:31463/150‑1 (MQ=60)
CGGTGTCGAGATAGATGGTGGAGGCCGTCAGCGGGGTGTCGGCGCGGATCGCCTGGATGGCTTGGATATCCATGGAACGTCTCGGGTAATGACAGGCGGGACGCGGCGACCGCGCCCCGAGGTAAGACTCAGTTGCGCACTTACTTGCGC < GWNJ‑0478:712:GW2002102894th:2:1115:2096:21676/150‑1 (MQ=60)
ttcaagcagaagacggcatacgagattagcgagtgtctcgtgggctcggagatgtgtataagagacagCCATGGAACGTCTCGGGTAATGACAGGCGGGACGCGGCGACCGCGCCCCGAGGTAAGACTCAGTTGCGCACTTACTTGCGC < GWNJ‑0478:712:GW2002102894th:2:2114:8422:34235/81‑1 (MQ=60)
ATAGATGGTGGAGGCCGTCAGCGGGGTGTCGGCGCGGATCGCCTGGATGGCTTGGATATCCATGGAACGTCTCGGGTAATGACAGGCGGGACGCGGCGACCGCGCCCCGAGGTAAGACTCAGTTGCGCACTTACTTGCGCAGCTTGGTGA < GWNJ‑0478:712:GW2002102894th:2:2105:9942:99204/150‑1 (MQ=60)
AGATGGTGGAGGCCGTCAGCGGGGTGTCGGCGCGGATCGCCTGGATGGCTTGGATATCCATGGAACGTCTCGGGTAATGACAGGCGGGACGCGGCGACCGCGCCCCGAGGTAAGACTCAGTTGCGCACTTACTTGCGCAGCTTGGTGACG > GWNJ‑0478:712:GW2002102894th:2:1214:8436:90307/1‑150 (MQ=60)
AGGCCGTCAGCGGGGTGTCGGCGCGGATCGCCTGGATGGCTTGGATATCCATGGAACGTCTCGGGTAATGACAGGCGGGACGCGGCGACCGCGCCCCGAGGTAAGACTCAGTTGCGCACTTACTTGCGCAGCTTGGTGACGGAGCCGAAG < GWNJ‑0478:712:GW2002102894th:2:2212:19649:58107/150‑1 (MQ=60)
aagagacagCGCCTGGATGGCTTGGATATCCATGGAACGTCTCGGGTAATGACAGGCGGGACGCGGCGACCGCGCCCCGAGGTAAGACTCAGTTGCGCACTTACTTGCGCAGCTTGGTGACGGAGCCGAAGAAGCTGAACAGGGCGTCGC < GWNJ‑0478:712:GW2002102894th:2:2204:15603:10471/141‑1 (MQ=60)
GGCTTGGATATCCATGGAACGTCTCGGGTAATGACAGGCGGGACGCGGCGACCGCGCCCCGAGGTAAGACctgtctcttatacacatctccgagcccacgagacactcgctaatctcgtatgccgtcttctgcttgaaaaaaaaaaaaa > GWNJ‑0478:712:GW2002102894th:2:1101:12359:6650/1‑70 (MQ=60)
TCCATGGAACGTCTCGGGTAATGACAGGCGGGACGCGGCGACCGCGCCCCGAGGTAAGACTCAGTTGCGCACTTACTTGCGCAGCTTGGTGACGGAGCCGAAGAAGCTGAACAGGGCGTCGCCGGCAATCACGCCGCCGGCGAAGGCGCT > GWNJ‑0478:712:GW2002102894th:2:2203:15889:96415/1‑150 (MQ=60)
GGTAATGACAGGCGGGACGCGGCGACCGCGCCCCGAGGTAAGACTCAGTTGCGCACTTACTTGCGCAGCTTGGTGACGGAGCCGAAGctgtctcttatacacatctccgagcccacgagacactcgctaatctcgtatgccgtcttctgc > GWNJ‑0478:712:GW2002102894th:2:2201:8263:21139/1‑87 (MQ=60)
GGTAATGACAGGCGGGACGCGGCGACCGCGCCCCGAGGTAAGACTCAGTTGCGCACTTACTTGCGCAGCTTGGTGACGGAGCCGAAGAAGCTGAACctgtctcttatacacatctccgagcccacgagacactcgctaatctcgtatgcc > GWNJ‑0478:712:GW2002102894th:2:2113:19431:58810/1‑96 (MQ=60)
TGACAGGCGGGACGCGGCGACCGCGCCCCGAGGTAAGACTCAGTTGCGCACTTACTTGCGCAGCTTGGTGACGGAGCCGAAGAAGCTGAACAGGGCGTCGCCGGCAATCACGCCGCCGGCGAAGGCGCTCATCTCGGCCACGTGCTTGTC > GWNJ‑0478:712:GW2002102894th:2:2115:12217:46274/1‑150 (MQ=60)
|
GCTGCGCACGGTATCGAGCACCGCGTCGGGCGGCAACGAGGCCGCGGCGGTGTCGAGATAGATGGTGGAGGCCGTCAGCGGGGTGTCGGCGCGGATCGCCTGGATGGCTTGGATATCCATGGAACGTCTCGGGTAATGACAGGC‑GGACGCGGCGACCGCGCCCCGAGGTAAGACTCAGTTGCGCACTTACTTGCGCAGCTTGGTGACGGAGCCGAAGAAGCTGAACAGGGCGTCGCCGGCAATCACGCCGCCGGCGAAGGCGCTCATCTCGGCCACGTGCTTGTC > AM260480/156951‑157235
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
