Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A0 F0 I1 R1
|
149 |
26.7 |
2337422 |
87.5% |
2045244 |
105.0 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
AM260480 |
635,877 |
T→C |
E259E (GAA→GAG) |
fliM ← |
flagellar motor switch protein |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | AM260480 | 635,877 | 0 | T | C | 100.0%
| 147.0
/ NA
| 39 | E259E (GAA→GAG) | fliM | flagellar motor switch protein |
| Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (20/19); total (20/19) |
GCACGCCGTCCACCTTGCCGAACACCTTCTCCGGCAGCTCGATCGGCAGCACGTCGCCGGCGCGCATCGCCAGCACTTCGGCCACGGTGCTCTGCAGGTGCGCGAACTCGGCCACCAGCTCCACTTCGGCCGCGCGCAGCTGCGTGGACAGTTGCGTGACCCAGCCCTTGTCGACTTCCTTCTCGTCCTGCAGCGGATTCATCAGCAGGTCCTTGAGCGGCTCGATCATCGCGTACGG > AM260480/635753‑635990
|
gCACGCCGTCCACCTTGCCGAACACCTTCTCCGGCAGCTCGATCGGCAGCACGTCGCCGGCGCGCATCGCCAGCACTTCGGCCACGGTGCTCTGCAGGTGCGCGAACTCGGCCACCAGCTCCACCTCGGCcgcg < 1:1005259/134‑1 (MQ=255)
aCCTTGCCGAACACCTTCTCCGGCAGCTCGATCGGCAGCACGTCGCCGGCGCGCATCGCCAGCACTTCGGCCACGGTGCTCTGCAGGTGCGCGAACTCGGCCACCAGCTCCACCTc > 1:696718/1‑116 (MQ=255)
aCCTTGCCGAACACCTTCTCCGGCAGCTCGATCGGCAGCACGTCGCCGGCGCGCATCGCCAGCACTTCGGCCACGGTGCTCTGCAGGTGCGCGAACTCGGCCACCAGCTCCACCTCGGCcg < 2:33277/121‑1 (MQ=255)
aCCTTGCCGAACACCTTCTCCGGCAGCTCGATCGGCAGCACGTCGCCGGCGCGCATCGCCAGCACTTCGGCCACGGTGCTCTGCAGGTGCGCGAACTCGGCCACCAGCTCCACCTCGGCcg > 1:33277/1‑121 (MQ=255)
ctcCGGCAGCTCGATCGGCAGCACGTCGCCGGCGCGCATCGCCAGCACTTCGGCCACGGTGCTCTGCAGGTGCGCGAACTCGGCCACCAGCTCCACCTCGGCCGCGCGCAGCTGCGTGGACAGTTGCGTGAc < 1:771663/132‑1 (MQ=255)
ctcCGGCAGCTCGATCGGCAGCACGTCGCCGGCGCGCATCGCCAGCACTTCGGCCACGGTGCTCTGCAGGTGCGCGAACTCGGCCACCAGCTCCACCTCGGCCGCGCGCAGCTGCGTGGACAGTTGCGTGAc < 1:947785/132‑1 (MQ=255)
gCTCGATCGGCAGCACGTCGCCGGCGCGCATCGCCAGCACTTCGGCCACGGTGCTCTGCAGGTGCGCGAACTCGGCCACCAGCTCCACCTCGGCCGCGCGCAGCTGCGTGGACAGTTGCGTg > 2:952430/1‑122 (MQ=255)
gCTCGATCGGCAGCACGTCGCCGGCGCGCATCGCCAGCACTTCGGCCACGGTGCTCTGCAGGTGCGCGAACTCGGCCACCAGCTCCACCTCGGCCGCGCGCAGCTGCGTGGACAGTTGCGTg < 1:952430/122‑1 (MQ=255)
cTCGATCGGCAGCACGTCGCCGGCGCGCATCGCCAGCACTTCGGCCACGGTGCTCTGCAGGTGCGCGAACTCGGCCACCAGCTCCACCTCGGCCGCGCGCAGCTGCGTGGACAGTTGCGTGACCCAGCCCTTGt < 1:389414/134‑1 (MQ=255)
tCGGCAGCACGTCGCCGGCGCGCATCGCCAGCACTTCGGCCACGGTGCTCTGCAGGTGCGCGAACTCGGCCACCAGCTCCACCTCGGCCGCGCGCAGCTGCGTGGACAGTTGCGTGAc < 2:892472/118‑1 (MQ=255)
tCGGCAGCACGTCGCCGGCGCGCATCGCCAGCACTTCGGCCACGGTGCTCTGCAGGTGCGCGAACTCGGCCACCAGCTCCACCTCGGCCGCGCGCAGCTGCGTGGACAGTTGCGTGAc > 1:892472/1‑118 (MQ=255)
ggCAGCACGTCGCCGGCGCGCATCGCCAGCACTTCGGCCACGGTGCTCTGCAGGTGCGCGAACTCGGCCACCAGCTCCACCTCGGCCGCGCGCAGCTGCGTGGACAGTTGCGTGACCCAGCCCTTGTCGActtc < 2:761441/134‑1 (MQ=255)
ggCAGCACGTCGCCGGCGCGCATCGCCAGCACTTCGGCCACGGTGCTCTGCAGGTGCGCGAACTCGGCCACCAGCTCCACCTCGGCCGCGCGCAGCTGCGTGGACAGTTGCGTGACCCAGCCCTTGTCGActtc < 2:1089590/134‑1 (MQ=255)
cGGCGCGCATCGCCAGCACTTCGGCCACGGTGCTCTGCAGGTGCGCGAACTCGGCCACCAGCTCCCCCTCGGCCGCGCGCAGCTGCGTGGACAGTTGCGTGACCCAGCCCTTGTCGACTTCCTTCTCGTCCTGc < 2:684277/134‑1 (MQ=255)
ggCGCGCATCGCCAGCACTTCGGCCACGGTGCTCTGCAGGTGCGCGAACTCGGCCACCAGCTCCACCTCGGCCGCGCGCAGCTGCGTGGACAGTTGCGTGACCCAGCCCTTGTCg > 2:53411/1‑115 (MQ=255)
ggCGCGCATCGCCAGCACTTCGGCCACGGTGCTCTGCAGGTGCGCGAACTCGGCCACCAGCTCCACCTCGGCCGCGCGCAGCTGCGTGGACAGTTGCGTGACCCAGCCCTTGTCg < 1:53411/115‑1 (MQ=255)
cATCGCCAGCACTTCGGCCACGGTGCTCTGCAGGTGCGCGAACTCGGCCACCAGCTCCACCTCGGCCGCGCGCAGCTGCGTGGACAGTTGCGTGACCCAGCCCTTGTCGACTTCCTTCTCGTCCTGCAGCGGAt > 2:1143135/1‑134 (MQ=255)
gCCAGCACTTCGGCCACGGTGCTCTGCAGGTGCGCGAACTCGGCCACCAGCTCCACCTCGGCCGCGCGCAGCTGCGTGGACAGTTGCGTGACCCAGCCCTTGTCGACTTCCTTCTCGTCCTGCAGCGGATtcat < 1:1143135/134‑1 (MQ=255)
cACGGTGCTCTGCAGGTGCGCGAACTCGGCCACCAGCTCCACCTCGGCcgcg < 2:577796/52‑1 (MQ=255)
cACGGTGCTCTGCAGGTGCGCGAACTCGGCCACCAGCTCCACCTCGGCcgcg > 1:577796/1‑52 (MQ=255)
cACGGTGCTCTGCAGGTGCGCGAACTCGGCCACCAGCTCCACCTCGGCCGCGCGCAGCTGCGTGGACAGt < 1:908038/70‑1 (MQ=255)
cACGGTGCTCTGCAGGTGCGCGAACTCGGCCACCAGCTCCACCTCGGCCGCGCGCAGCTGCGTGGACAGt > 2:908038/1‑70 (MQ=255)
aGGTGCGCGAACTCGGCCACCAGCTCCACCTCGGCCGCGCGCAGCTGCGTGGACAGTTGCGTGACCCAGCCCTTGTCGACTTCCTTCTCGTCCTGCAGCGGATTCATCAGCAGGTCCTTGAGCGGCTCGatcat > 1:670009/1‑134 (MQ=255)
gcgAACTCGGCCACCAGCTCCACCTCGGCCGCGCGCAGCTGCGTGGACAGTTGCGTGACCCAGCCCTTGTCGACTTCCTTCTCGTCCTGCAg < 2:465114/92‑1 (MQ=255)
gcgAACTCGGCCACCAGCTCCACCTCGGCCGCGCGCAGCTGCGTGGACAGTTGCGTGACCCAGCCCTTGTCGACTTCCTTCTCGTCCTGCAg > 1:465114/1‑92 (MQ=255)
gcgAACTCGGCCACCAGCTCCACCTCGGCCGCGCGCAGCTGCGTGGACAGTTGCGTGACCCAGCCCTTGTCGACTTCCTTCTCGTCCTGCAGCGGATtcatc > 2:467053/1‑102 (MQ=255)
gcgAACTCGGCCACCAGCTCCACCTCGGCCGCGCGCAGCTGCGTGGACAGTTGCGTGACCCAGCCCTTGTCGACTTCCTTCTCGTCCTGCAGCGGATtcatc < 1:467053/102‑1 (MQ=255)
gcgAACTCGGCCACCAGCTCCACCTCGGCCGCGCGCAGCTGCGTGGACAGTTGCGTGACCCAGCCCTTGTCGACTTCCTTCTCGTCCTGCAGCGGATTCATCAGCAGGTCCTTGAGCGGCTCGATCATCGCGTa > 1:274204/1‑134 (MQ=255)
gAACTCGGCCACCAGCTCCACCTCGGCCGCGCGCAGCTGCGTGGACAGTTGCGTGACCCAGCCCTTGTCGACTTCCTTCTCg > 2:411386/1‑82 (MQ=255)
gAACTCGGCCACCAGCTCCACCTCGGCCGCGCGCAGCTGCGTGGACAGTTGCGTGACCCAGCCCTTGTCGACTTCCTTCTCg < 1:411386/82‑1 (MQ=255)
gAACTCGGCCACCAGCTCCACCTCGGCCGCGCGCAGCTGCGTGGACAGTTGCGTGACCCAGCCCTTGTCGACTTCCTTCTCGTCCTGCAGCGGa > 2:390165/1‑94 (MQ=255)
gAACTCGGCCACCAGCTCCACCTCGGCCGCGCGCAGCTGCGTGGACAGTTGCGTGACCCAGCCCTTGTCGACTTCCTTCTCGTCCTGCAGCGGa < 1:390165/94‑1 (MQ=255)
gAACTCGGCCACCAGCTCCACCTCGGCCGCGCGCAGCTGCGTGGACAGTTGCGTGACCCAGCCCTTGTCGACTTCCTTCTCGTCCTGCAGCGGATTCATCAGCAGGTCCTTGAGCGGCTCGATCATCGCGTACg > 2:356343/1‑134 (MQ=255)
aaCTCGGCCACCAGCTCCACCTCGGCCGCGCGCAg < 2:877722/35‑1 (MQ=37)
aaCTCGGCCACCAGCTCCACCTCGGCCGCGCGCAg > 1:877722/1‑35 (MQ=255)
aaCTCGGCCACCAGCTCCACCTCGGCCGCGCGCAGCTGCGTGGACAGTTGCGTGACCCAGCCCTTGTCGACTTCCTTCTCGTCCTGCAGCGGATTCATCAGCAGGTCCTTGAGCGGCTCGATCATCGCGTACgg > 1:905685/1‑134 (MQ=255)
cTCGGCCACCAGCTCCACCTCGGCCGCGCGCAGCTGCGTGGACAGTTGCGTGACCCAGCCCTTGTCGACTTCCTTCTCGTCCTGCAGCGGATTCATCAGCAGGTCCTTGAGCGGCTCGATCATCGCGTACgg > 1:858247/1‑132 (MQ=255)
cTCGGCCACCAGCTCCACCTCGGCCGCGCGCAGCTGCGTGGACAGTTGCGTGACCCAGCCCTTGTCGACTTCCTTCTCGTCCTGCAGCGGATTCATCAGCAGGTCCTTGAGCGGCTCGATCATCGCGTACgg > 2:58363/1‑132 (MQ=255)
cTCGGCCACCAGCTCCACCTCGGCCGCGCGCAGCTGCGTGGACAGTTGCGTGACCCAGCCCTTGTCGACTTCCTTCTCGTCCTGCAGCGGATTCATCAGCAGGTCCTTGAGCGGCTCGATCATCGCGTACgg > 1:1147746/1‑132 (MQ=255)
|
GCACGCCGTCCACCTTGCCGAACACCTTCTCCGGCAGCTCGATCGGCAGCACGTCGCCGGCGCGCATCGCCAGCACTTCGGCCACGGTGCTCTGCAGGTGCGCGAACTCGGCCACCAGCTCCACTTCGGCCGCGCGCAGCTGCGTGGACAGTTGCGTGACCCAGCCCTTGTCGACTTCCTTCTCGTCCTGCAGCGGATTCATCAGCAGGTCCTTGAGCGGCTCGATCATCGCGTACGG > AM260480/635753‑635990
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
BRESEQ :: bam2aln output
GAAGCCGCATTGCATGACGGGCACGCCGTCCACCTTGCCGAACACCTTCTCCGGCAGCTCGATCGGCAGCACGTCGCCGGCGCGCATCGCCAGCACTTCGGCCACGGTGCTCTGCAGGTGCGCGAACTCGGCCACCAGCTCCACTTCGGCCGCGCGCAGCTGCGTGGACAGTTGCGTGACCCAGCCCTTGTCGACTTCCTTCTCGTCCTGCAGCGGATTCATCAGCAGGTCCTTGAGCGGCTCGATCATCGCGTACGGCAGGCACACGTGGAGCTGCCCGCCGACGG > AM260480/635733‑636019
|
gtacaggggtccattgtgtttgactattgatttttttttttttttcaagcagaagacggcatacgagattagcgagtgtctcgtgggctcggagatgtgtataagagacagCTGCAGGTGCGCGAACTCGGCCACCAGCTCCACCTCGGC < GWNJ‑0478:712:GW2002102894th:2:2104:19188:87449/39‑1 (MQ=60)
tattaattttttgtttttttttttttttttttcaagcagaagacggcatacgagattagcgagtgtctcgtgggctcggagatgtgtataagagacagCTGCAGGTGCGCGAACTCGGCCACCAGCTCCACCTCGGCCGCGCGCAGCTG < GWNJ‑0478:712:GW2002102894th:2:2213:18155:51235/51‑1 (MQ=60)
ACGGGCACGCCGTCCACCTTGCCGAACACCTTCTCCGGCAGCTCGATCGGCAGCACGTCGCCGGCGCGCATCGCCAGCACTTCGGCCACGGTGCTCTGCAGGTGCGCGAACTCGGCCACCAGCTCCACCTCGGCCGCGCGCAGCTGCtgt > GWNJ‑0478:712:GW2002102894th:2:2108:13520:39886/1‑147 (MQ=60)
ACGGGCACGCCGTCCACCTTGCCGAACACCTTCTCCGGCAGCTCGATCGGCAGCACGTCGCCGGCGCGCATCGCCAGCACTTCGGCCACGGTGCTCTGCAGGTGCGCGAACTCGGCCACCAGCTCCACCTCGGCCGCGCGCAGCTGCGTG > GWNJ‑0478:712:GW2002102894th:2:1105:7169:13959/1‑150 (MQ=60)
GGCACGCCGTCCACCTTGCCGAACACCTTCTCCGGCAGCTCGATCGGCAGCACGTCGCCGGCGCGCATCGCCAGCACTTCGGCCACGGTGCTCTGCAGGTGCGCGAACTCGGCCACCAGCTCCACCTCGGCCGCGCGCAGCTGCGTGGAC < GWNJ‑0478:712:GW2002102894th:2:2208:9870:7432/150‑1 (MQ=60)
acgagattagcgagtgtctcgtgggctcggagatgtgtataagagacagCGCCAGCACTTCGGCCACGGTGCTCTGCAGGTGCGCGAACTCGGCCACCAGCTCCACCTCGGCCGCGCGCAGCTGCGTGGACAGTTGCGTGACCCAGCCC < GWNJ‑0478:712:GW2002102894th:2:2202:15666:41945/100‑1 (MQ=60)
ACCTTCTCCGGCAGCTCGATCGGCAGCACGTCGCCGGCGCGCATCGCCAGCACTTCGGCCACGGTGCTCTGCAGGTGCGCGAACTCGGCCACCAGCTCCACCTCGGCCGCGCGCAGCTGCGTGGACAGTTGCGTGACCCAGCCCTTGTCG < GWNJ‑0478:712:GW2002102894th:2:2209:7563:9895/150‑1 (MQ=60)
TCTCCGGCAGCTCGATCGGCAGCACGTCGCCGGCGCGCATCGCCAGCACTTCGGCCACGGTGCTCTGCAGGTGCGCGAACTCGGCCACCAGCTCCACCTCGGCCGCGCGCAGCTGCGTGGACAGTTGCGTGACCCAGCCCTTGTCGACCT > GWNJ‑0478:712:GW2002102894th:2:2203:13307:46143/1‑150 (MQ=60)
TCTCCGGCAGCTCGATCGGCAGCACGTCGCCGGCGCGCATCGCCAGCACTTCGGCCACGGTGCTCTGCAGGTGCGCGAACTCGGCCACCAGCTCCACCTCGGCCGCGCGCAGCTGCGTGGACAGTTGCGTGACCCAGCCCTTGTCGAC < GWNJ‑0478:712:GW2002102894th:2:2113:18837:33697/148‑1 (MQ=60)
TCTCCGGCAGCTCGATCGGCAGCACGTCGCCGGCGCGCATCGCCAGCACTTCGGCCACGGTGCTCTGCAGGTGCGCGAACTCGGCCACCAGCTCCACCTCGGCCGCGCGCAGCTGCGTGGACAGTTGCGTGACCCAGCCCTTGTCGAC < GWNJ‑0478:712:GW2002102894th:2:2205:9422:82557/148‑1 (MQ=60)
GCTCGATCGGCAGCACGTCGCCGGCGCGCATCGCCAGCACTTCGGCCACGGTGCTCTGCAGGTGCGCGAACTCGGCCACCAGCTCCACCTCGGCCGCGCGCAGCTGCGTGGACAGTTGCGTGACCCAGCCCTTGTCGACTTCCTTCTCGT < GWNJ‑0478:712:GW2002102894th:2:1206:16089:56179/150‑1 (MQ=60)
gacagCGGCAGCACGTCGCCGGCGCGCATCGCCAGCACTTCGGCCACGGTGCTCTGCAGGTGCGCGAACTCGGCCACCAGCTCCACCTCGGCCGCGCGCAGCTGCGTGGACAGTTGCGTGACCCAGCCCTTGTCGACTTCCTTCTCGTCC < GWNJ‑0478:712:GW2002102894th:2:1102:6817:41714/145‑1 (MQ=60)
gagtgtctcgtgggctcggagatgtgtataagagacagGCTCTGCAGGTGCGCGAACTCGGCCACCAGCTCCACCTCGGCCGCGCGCAGCTGCGTGGACAGTTGCGTGACCCAGCCCTTGTCGACTTCCTTCTCGTCCTGCAGCGGATTC < GWNJ‑0478:712:GW2002102894th:2:1208:3930:81766/112‑1 (MQ=60)
ggctcggagatgtgtataagagacagGCTCTGCAGGTGCGCGAACTCGGCCACCAGCTCCACCTCGGCCGCGCGCAGCTGCGTGGACAGTTGCGTGACCCAGCCCTTGTCGACTTCCTTCTCGTCCTGCAGCGGATTCATCAGCAGGTCC < GWNJ‑0478:712:GW2002102894th:2:1206:13753:60990/124‑1 (MQ=60)
CGCCAGCACTTCGGCCACGGTGCTCTGCAGGTGCGCGAACTCGGCCACCAGCTCCACCTCGGCCGCGCGCAGCTGCGTGGACctgtctcttatacacatctccgagcccacgagacactcgctaacctcgcatgccgtcttctgctaga > GWNJ‑0478:712:GW2002102894th:2:2101:9004:22318/1‑82 (MQ=60)
CGCCAGCACTTCGGCCACGGTGCTCTGCAGGTGCGCGAACTCGGCCACCAGCTCCACCTCGGCCGCGCGCAGCTGCGTGGACAGTTGCGTGACCCAGCCCTTGTCGACTTCCTTCTCGTCCTGCAGCGGATTCATCAGCAGGTCCTTGAG < GWNJ‑0478:712:GW2002102894th:2:2215:5992:89673/150‑1 (MQ=60)
gatgtgtataagagacaGGTGCTCTGCAGGTGCGCGAACTCGGCCACCAGCTCCACCTCGGCCGCGCGCAGCTGCGTGGACAGTTGCGTGACCCAGCCCTTGTCGACTTCCTTCTCGTCCTGCAGCGGATTCATCAGCAGGTCCTTGAGC < GWNJ‑0478:712:GW2002102894th:2:1210:5754:83719/133‑1 (MQ=60)
GCCACGGTGCTCTGCAGGTGCGCGAACTCGGCCACCAGCTCCACCTCGGCCGCGCGCAGCTGCGTGGACAGTTGCGTGACCCAGCCCTTGTCGACTTCCTTCTCGTCCTGCAGCGGATTCATCAGCAGGTCCTTGAGCGGCTCGATCATC > GWNJ‑0478:712:GW2002102894th:2:2107:15330:82410/1‑150 (MQ=60)
GTGCTCTGCAGGTGCGCGAACTCGGCCACCAGCTCCACCTCGGCCtgtctcttatacacatctccgagcccacgagacactcgctaatctcgtatgccgtcttctgcttgaaaaaaaaaaaaaaagacagcacaagaagataacaacaa > GWNJ‑0478:712:GW2002102894th:2:2216:8997:65489/1‑45 (MQ=60)
GTGCTCTGCAGGTGCGCGAACTCGGCCACCAGCTCCACCTCGGCCGCGCGCAGCTGCGTGGACAGTTGCGTGACCCAGCCCTTGTCGACTTCCTTCTCGTCCTGCAGCGGATTCATCAGCAGctgtctcttatacacatctccgagccc > GWNJ‑0478:712:GW2002102894th:2:1210:2713:73128/1‑122 (MQ=60)
GTGCTCTGCAGGTGCGCGAACTCGGCCACCAGCTCCACCTCGGCCGCGCGCAGCTGCGTGGACAGTTGCGTGACCCAGCCCTTGTCGACTTCCTTCTCGTCCTGCAGCGGATTCATCAGCAGGTCCTTGAGCGGCTCGATCATCGCGTAC > GWNJ‑0478:712:GW2002102894th:2:1116:8088:49301/1‑150 (MQ=60)
CTCTGCAGGTGCGCGAACTCGGCCACCAGCTCCACCTCGGCCGCGCGCAGCTGCGTGGACAGTTGCtgtctcttatacacatctccgagcccacgagacactcgctaatctcgtatgccgtcttctgcttgaaaaaaaaaaaaaaaaact > GWNJ‑0478:712:GW2002102894th:2:2201:20412:53712/1‑66 (MQ=60)
CTCTGCAGGTGCGCGAACTCGGCCACCAGCTCCACCTCGGCCGCGCGCAGCTGCGTGGACAGTTGCGTGACCCAGCCCTTGTCGACTTCCTTCTCGTCCTGCAGCGGATTCATCAGCAGGTCCTTGAGCGGCTCGATCATCGCGTACGGC > GWNJ‑0478:712:GW2002102894th:2:2202:9993:27321/1‑150 (MQ=60)
CTGCAGGTGCGCGAACTCGGCCACCAGCTCCACCTCGGCCGCGCGCAGCTGCGTGGACAGTTGCGTGACCCAGCCCTTGTCGACTTCCTTCTCGTCCTGCAGCGGATTCATCAGCAGGTCCTTGAGCGGCTCGATCATCGCGTACGGC > GWNJ‑0478:712:GW2002102894th:2:2116:12845:47025/1‑148 (MQ=60)
CTGCAGGTGCGCGAACTCGGCCACCAGCTCCACCTCGGCCGCGCGCAGCTGCGTGGACAGTTGCGTGACCCAGCCCTTGTCGACTTCCTTCTCGTCCTGCAGCGGATTCATCAGCAGGTCCTTGAGCGGCTCGATCATCGCGTACGGC > GWNJ‑0478:712:GW2002102894th:2:2216:1413:9808/1‑148 (MQ=60)
CACCAGCTCCACCTCGGCCGCGCGCAGCTGCGTGGACAGTTGCGTGACCCAGCCCTTGTCGACTTCCTTCTCGTCCTGCAGCGGATTCATCAGCAGGTCCTTGAGCGGCTCGATCATCGCGTACGGCAGGCACACGTGGAGCTGCCCGCC > GWNJ‑0478:712:GW2002102894th:2:2211:12529:99079/1‑150 (MQ=60)
GCTCCACCTCGGCCGCGCGCAGCTGCGTGGACAGTTGCGTGACCCAGCCCTTGTCGACTTCCTTCTCGTCCTGCAGCGGATTCATCAGCAGGTCCTTGAGCGGCTCGATCATCGCtgtctcttatacacatctccgagcccacgagacac > GWNJ‑0478:712:GW2002102894th:2:1213:7284:28037/1‑115 (MQ=60)
GCTCCACCTCGGCCGCGCGCAGCTGCGTGGACAGTTGCGTGACCCAGCCCTTGTCGACTTCCTTCTCGTCCTGCAGCGGATTCATCAGCAGGTCCTTGAGCGGCTCGATCATCGCGTACGGCAGGCACACGTGGAGCTGCCCGCCGACGG > GWNJ‑0478:712:GW2002102894th:2:2101:20659:27419/1‑150 (MQ=60)
|
GAAGCCGCATTGCATGACGGGCACGCCGTCCACCTTGCCGAACACCTTCTCCGGCAGCTCGATCGGCAGCACGTCGCCGGCGCGCATCGCCAGCACTTCGGCCACGGTGCTCTGCAGGTGCGCGAACTCGGCCACCAGCTCCACTTCGGCCGCGCGCAGCTGCGTGGACAGTTGCGTGACCCAGCCCTTGTCGACTTCCTTCTCGTCCTGCAGCGGATTCATCAGCAGGTCCTTGAGCGGCTCGATCATCGCGTACGGCAGGCACACGTGGAGCTGCCCGCCGACGG > AM260480/635733‑636019
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
