Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F1 I1 R1
|
150 |
26.0 |
2053212 |
90.0% |
1847890 |
107.8 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
AM260479 |
628,654 |
+C |
coding (744/1206 nt) |
h16_A0595 ← |
Uncharacterized Fe‑S protein |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | AM260479 | 628,653 | 1 | . | C | 100.0%
| 54.1
/ NA
| 15 | T249A (ACG→GCG) | h16_A0595 | Uncharacterized Fe‑S protein |
| Reads supporting (aligned to +/- strand): ref base . (0/0); new base C (8/7); total (8/7) |
CAGGCCAGCTGGCAGTCGTCGCAGCCGTAGACGCGGTTGCCCATGGGCGCGCGGAACTCCACCGGAATCGGGCCCTTGTGCTCGATGGTCAGGTAGGAGATGCAAGCGGCGCGCGT‑CAGCCGGTACGGCGCCACGATGGCGCGCGTGGGGCAGATGTCCAGGCAGCGGTGGCAGTTGCCGCAGTGGCCGGACTCGGGGGCGTCGGCCGGCAGCGGGATGTCGACCAGG > AM260479/628538‑628765
|
cAGGCCAGCTGGCAGTCGTCGCAGCCGTAGACGCGGTTGCCCATGGGCGCGCGGAACTCCACCGGAATCGGGCCCTTGTGCTCGATGGTCAGGTAGGAGATGC‑AGCGGCGCGCGT‑c < 2:66181/116‑1 (MQ=255)
cAGGCCAGCTGGCAGTCGTCGCAGCCGTAGACGCGGTTGCCCATGGGCGCGCGGAACTCCACCGGAATCGGGCCCTTGTGCTCGATGGTCAGGTAGGAGATGC‑AGCGGCGCGCGT‑c > 1:66181/1‑116 (MQ=255)
cAGCTGGCAGTCGTCGCAGCCGTAGACGCGGTTGCCCATGGGCGCGCGGAACTCCACCGGAATCGGGCCCTTGTGCTCGATGGTCAGGTAGGAGATGC‑AGCGGCGCGCGTCCAGCCGGTACGGCGCCACGATgg > 1:581816/1‑134 (MQ=255)
gtcgCAGCCGTAGACGCGGTTGCCCATGGGCGCGCGGAACTCCACCGGAATCGGGCCCTTGTGCTCGATGGTCAGGTAGGAGATGC‑AGCGGCGCGCGTCCAGCCGGTACGGCGCCACGATGGCGCGCGTgg > 1:193393/1‑131 (MQ=255)
gtcgCAGCCGTAGACGCGGTTGCCCATGGGCGCGCGGAACTCCACCGGAATCGGGCCCTTGTGCTCGATGGTCAGGTAGGAGATGC‑AGCGGCGCGCGTCCAGCCGGTACGGCGCCACGATGGCGCGCGTgg < 2:193393/131‑1 (MQ=255)
tGGGCGCGCGGAACTCCACCGGAATCGGGCCCTTGTGCTCGATGGTCAGGTAGGAGATGC‑AGCGGCGCGCGTCCAGCCGGt < 1:143323/81‑1 (MQ=255)
tGGGCGCGCGGAACTCCACCGGAATCGGGCCCTTGTGCTCGATGGTCAGGTAGGAGATGC‑AGCGGCGCGCGTCCAGCCGGt > 2:143323/1‑81 (MQ=255)
aTCGGGCCCTTGTGCTCGATGGTCAGGTAGGAGATGC‑AGCGGCGCGCGTCCAGCCGGTACGGCGCCACGATGGCGCGCGTGGGGCAGATGTCCAg > 1:447282/1‑95 (MQ=255)
aTCGGGCCCTTGTGCTCGATGGTCAGGTAGGAGATGC‑AGCGGCGCGCGTCCAGCCGGTACGGCGCCACGATGGCGCGCGTGGGGCAGATGTCCAg < 2:447282/95‑1 (MQ=255)
gCCCTTGTGCTCGATGGTCAGGTAGGAGATGC‑AGCGGCGCGCGTCCAGCCGGTACGGCGCCACGATGGCGCGCGTGGGGCAGATGTCCAGGCAGCGGTGGCAGTTGCCGCAGTGGCCGGACTCGGGGGCGTcgg > 1:733110/1‑134 (MQ=255)
gCCCTTGTGCTCGATGGTCAGGTAGGAGATGC‑AGCGGCGCGCGTCCAGCCGGTACGGCGCCACGATGGCGCGCGTGGGGCAGATGTCCAGGCAGCGGTGGCAGTTGCCGCAGTGGCCGGACTCGGGGGCGTcgg > 1:547768/1‑134 (MQ=255)
gCCCTTGTGCTCGATGGTCAGGTAGGAGATGC‑AGCGGCGCGCGTCCAGCCGGTACGGCGCCACGATGGCGCGCGTGGGGCAGATGTCCAGGCAGCGGTGGCAGTTGCCGCAGTGGCCGGACTCGGGGGCGTcgg > 1:33110/1‑134 (MQ=255)
ccTTGTGCTCGATGGTCAGGTAGGAGATGC‑AGCGGCGCGCGTCCAGCCGGTACGGCGCCACGATGGCGCGCGTGGGGCAGATGTCCAGGCAGCGGTGGCAGTTGCCGCAGTGGCCGGACTCGGGGGCGTcggcc > 2:378426/1‑134 (MQ=255)
gTCAGGTAGGAGATGC‑AGCGGCGCGCGTCCAGCCGGTACGGCGCCACGATGGCGCGCGTGGGGCAGATGTCCAGGCAGCGGTGGCAGTTGCCGCAGTGGCCGGACTCGGGGGCGTCGGCCGGCAGCGGGATGt < 2:733110/133‑1 (MQ=255)
gTAGGAGATGC‑AGCGGCGCGCGTCCAGCCGGTACGGCGCCACGATGGCGCGCGTGGGGCAGATGTCCAGGCAGCGGTGGCAGTTGCCGCAGTGGCCGGACTCGGGGGCGTCGGCCGGCAGCGGGATGTCGACCa < 2:33110/134‑1 (MQ=255)
gTAGGAGATGC‑AGCGGCGCGCGTCCAGCCGGTACGGCGCCACGATGGCGCGCGTGGGGCAGATGTCCAGGCAGCGGTGGCAGTTGCCGCAGTGGCCGGACTCGGGGGCGTCGGCCGGCAGCGGGATGTCGACCa < 2:547768/134‑1 (MQ=255)
ggAGATGC‑AGCGGCGCGCGTCCAGCCGGTACGGCGCCACGATGGCGCGCGTGGGGCAGATGTCCAGGCAGCGGTGGCAGTTGCCGCAGTGGCCGGACTCGGGGGCGTCGGCCGGCAGCGGGATGTCGACCAgg < 1:1004004/133‑1 (MQ=255)
|
CAGGCCAGCTGGCAGTCGTCGCAGCCGTAGACGCGGTTGCCCATGGGCGCGCGGAACTCCACCGGAATCGGGCCCTTGTGCTCGATGGTCAGGTAGGAGATGCAAGCGGCGCGCGT‑CAGCCGGTACGGCGCCACGATGGCGCGCGTGGGGCAGATGTCCAGGCAGCGGTGGCAGTTGCCGCAGTGGCCGGACTCGGGGGCGTCGGCCGGCAGCGGGATGTCGACCAGG > AM260479/628538‑628765
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
BRESEQ :: bam2aln output
ACATGCGCGAACTTGTTCCACGGGCAGGCCAGCTGGCAGTCGTCGCAGCCGTAGACGCGGTTGCCCATGGGCGCGCGGAACTCCACCGGAATCGGGCCCTTGTGCTCGATGGTCAGGTAGGAGATGCAAGCGGCGCGCGT‑CAGCCGGTACGGCGCCACGATGGCGCGCGTGGGGCAGATGTCCAGGCAGCGGTGGCAGTTGCCGCAGTGGCCGGACTCGGGGGCGTCGGCCGGCAGCGGGATGTCGACCAGGATCTCGCCCAGGAAG > AM260479/628514‑628780
|
ACATGCGCGAACTTGTTCCACGGGCAGGCCAGCTGGCAGTCGTCGCAGCCGTAGACGCGGTTGCCCATGGGCGCGCGGAACTCCACCGGAATCGGGCCCTTGTGCTCGATGGTCAGGTAGGAGATGC‑AGCGGCGCGCGTCCAGCCGGTAC < GWNJ‑0478:712:GW2002102894th:2:1216:20957:20791/150‑1 (MQ=60)
AACTTGTTCCACGGGCAGGCCAGCTGGCAGTCGTCGCAGCCGTAGACGCGGTTGCCCATGGGCGCGCGGAACTCCACCGGAATCGGGCCCTTGTGCTCGATGGTCAGGTAGGAGATGC‑AGCGGCGCGCGTCCAGCCGGTACGGCGCCtgt > GWNJ‑0478:712:GW2002102894th:2:1104:3654:53203/1‑147 (MQ=60)
gctccgtctcgtgggctcggagatgtgtataagagacagAGACGCGGTTGCCCATGGGCGCGCGGAACTCCACCGGAATCGGGCCCTTGTGCTCGATGGTCAGGTAGGAGATGC‑AGCGGCGCGCGTCCAGCCGGTACGGCGCCACGATGG < GWNJ‑0478:712:GW2002102894th:2:1111:12883:41475/111‑1 (MQ=60)
GTTCCACGGGCAGGCCAGCTGGCAGTCGTCGCAGCCGTAGACGCGGTTGCCCATGGGCGCGCGGAACTCCACCGGAATCGGGCCCTTGTGCTCGATGGTCAGGTAGGAGATGC‑AGCGGCGCGCGTCCAGCCGGTACGGCGCCACGATGGC > GWNJ‑0478:712:GW2002102894th:2:2104:2244:71478/1‑150 (MQ=60)
GGCCAGCTGGCAGTCGTCGCAGCCGTAGACGCGGTTGCCCATGGGCGCGCGGAACTCCACCGGAATCGGGCCCTTGTGCTCGATGGTCAGGTAGGAGATGC‑AGCGGCGCGCGTCCAGCCGGTACGGCGCCACGATGGCGCGCGTGGGGC > GWNJ‑0478:712:GW2002102894th:2:1112:16238:41251/1‑149 (MQ=60)
CGGAACTCCACCGGAATCGGGCCCTTGTGCTCGATGGTCAGGTAGGAGATGC‑AGCGGCGCGCGTCCAGCCGGTACGGCGCCACGATGGCGCGCGTGGGGCAGATGTCCAGGCAGCGGTGGCAGTTctgtctcttatacacatctccgagc > GWNJ‑0478:712:GW2002102894th:2:1212:12895:21101/1‑125 (MQ=60)
CTCCACCGGAATCGGGCCCTTGTGCTCGATGGTCAGGTAGGAGATGC‑AGCGGCGCGCGTCCAGCCGGTACGGCGCCACGATGGCGCGCGTGGGGCAGATGTCCAGGCAGCGGTGGCAGTTGCCGCAGTGGCCGGACTCGGGGGCGTCGGC > GWNJ‑0478:712:GW2002102894th:2:1105:12104:28462/1‑150 (MQ=60)
CTCCACCGGAATCGGGCCCTTGTGCTCGATGGTCAGGTAGGAGATGC‑AGCGGCGCGCGTCCAGCCGGTACGGCGCCACGATGGCGCGCGTGGGGCAGATGTCCAGGCAGCGGTGGCAGTTGCCGCAGTGGCCGGACTCGGGGGCGTCGGC > GWNJ‑0478:712:GW2002102894th:2:2102:9799:38803/1‑150 (MQ=60)
CTCCACCGGAATCGGGCCCTTGTGCTCGATGGTCAGGTAGGAGATGC‑AGCGGCGCGCGTCCAGCCGGTACGGCGCCACGATGGCGCGCGTGGGGCAGATGTCCAGGCAGCGGTGGCAGTTGCCGCAGTGGCCGGACTCGGGGGCGTCGGC > GWNJ‑0478:712:GW2002102894th:2:2115:9950:99205/1‑150 (MQ=60)
AGGAGATGC‑AGCGGCGCGCGTCCAGCCGGTACGGCGCCACGATGGCGCGCGTGGGGCAGATGTCCAGGCAGCGGTGGCAGTTGCCGCAGTGGCCGGACTCGGGGGCGTCGGCCGGCAGCGGGATGTCGACCAGGATCTCGCCCAGGAAG < GWNJ‑0478:712:GW2002102894th:2:2215:20313:67444/149‑1 (MQ=60)
|
ACATGCGCGAACTTGTTCCACGGGCAGGCCAGCTGGCAGTCGTCGCAGCCGTAGACGCGGTTGCCCATGGGCGCGCGGAACTCCACCGGAATCGGGCCCTTGTGCTCGATGGTCAGGTAGGAGATGCAAGCGGCGCGCGT‑CAGCCGGTACGGCGCCACGATGGCGCGCGTGGGGCAGATGTCCAGGCAGCGGTGGCAGTTGCCGCAGTGGCCGGACTCGGGGGCGTCGGCCGGCAGCGGGATGTCGACCAGGATCTCGCCCAGGAAG > AM260479/628514‑628780
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
