Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F1 I1 R1
|
150 |
26.0 |
2053212 |
90.0% |
1847890 |
107.8 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
AM260479 |
1,071,935 |
+G |
coding (626/627 nt) |
h16_A0979 → |
Hypothetical protein |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | AM260479 | 1,071,934 | 1 | . | G | 100.0%
| 96.1
/ NA
| 26 | *209G (TGA→GGA) | h16_A0979 | Hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base . (0/0); new base G (16/10); total (16/10) |
CGCGCAACCTCTACCGGGCCGAGGTGGGGTTGTCCGTCGCCGGCGACAACGCGGCCCTGCGCGCGCTGTGGCCGGGCTGGACCGGCTTCGGCATGCAGGACCACACCGTGCTGCTGACCAACGCCT‑GACGCCCGAGGGGCGCAACGGCACGCTCGCCGTGATCAAGGAAGCGGTGCCCACCAGCAA‑GGCGGCGTGATCGACACCGCGCTGACCGTCGGGCTGG > AM260479/1071809‑1072031
|
cgcgCAACCTCTACCGGGCCGAGGTGGGGTTGTCCGTCGCCGGCGACAACGCGGCCCTGCGCGCGCTGTGGCCGGGCTGGACCGGCTTCGGCATGCAGGACCACACCGTGCTGCTGACCAACGCCTGGACGccc > 2:1026489/1‑134 (MQ=255)
cAACCTCTACCGGGCCGAGGTGGGGTTGTCCGTCGCCGGCGACAACGCGGCCCTGCGCGCGCTGTGGCCGGGCTGGACCGGCTTCGGCATGCAGGACCACACCGTGCTGCTGACCAACGCCTGGACGCCCGAgg > 1:89194/1‑134 (MQ=255)
gAGGTGGGGTTGTCCGTCGCCGGCGACAACGCGGCCCTGCGCGCGCTGTGGCCGGGCTGGACCGGCTTCGGCATGCAGGACCACACCGTGCTGCTGACCAACGCCTGGACGCCCGAGGGGCGCAACGGCACGCt < 1:333605/134‑1 (MQ=255)
aGGTGGGGTTGTCCGTCGCCGGCGACAACGCGGCCCTGCGCGCGCTGTGGCCGGGCTGGACCGGCTTCGGCATGCAGGACCACACCGTGCTGCTGACCAACGCCTGGACGCCCGAGGGGCGCAACGGCACGCTc > 2:50962/1‑134 (MQ=255)
ggggTTGTCCGTCGCCGGCGACAACGCGGCCCTGCGCGCGCTGTGGCCGGGCTGGACCGGCTTCGGCATGCAGGACCACACCGTGCTGCTGACCAACGCCTGGACGCCCGAGGGGCGCAACGGCACGCTCGCCg > 1:431576/1‑134 (MQ=255)
gTCCGTCGCCGGCGACAACGCGGCCCTGCGCGCGCTGTGGCCGGGCTGGACCGGCTTCGGCATGCAGGACCACACCGTGCTGCTGACCAACGCCTGGACGCCCGAGGGGCGCAACGGCACGCTCGCCGTGATc > 1:986358/1‑133 (MQ=255)
gTCGCCGGCGACAACGCGGCCCTGCGCGCGCTGTGGCCGGGCTGGACCGGCTTCGGCATGCAGGACCACACCGTGCTGCTGACCAACGCCTGGACGCCCGAGGGGCGCAACGGCACGCTCGCCGTGATCAAgg > 2:796134/1‑133 (MQ=255)
tCGCCGGCGACAACGCGGCCCTGCGCGCGCTGTGGCCGGGCTGGACCGGCTTCGGCATGCAGGACCACACCGTGCTGCTGACCAACGCCTGGACGCCCGAGGGGCGCAACGGCACGCTCGCCGTGATCAAgg > 2:918298/1‑132 (MQ=255)
gCCGGCGACAACGCGGCCCTGCGCGCGCTGTGGCCGGGCTGGACCGGCTTCGGCATGCAGGACCACACCGTGCTGCTGACCAACGCCTGGACGCCCGAGGGGCGCAACGGCACGCTCGCCGTGATCAAGGAAGc > 2:1006457/1‑134 (MQ=255)
gCGACAACGCGGCCCTGCGCGCGCTGTGGCCGGGCTGGACCGGCTTCGGCATGCAGGACCACACCGTGCTGCTGACCAACGCCTGGACGCCCGAGGGGCGCAACGGCACGCTCGCCGTGATCAAGGAAGCGGTg < 2:986358/134‑1 (MQ=255)
gCCCTGCGCGCGCTGTGGCCGGGCTGGACCGGCTTCGGCATGCAGGACCACACCGTGCTGCTGACCAACGCCTGGACGCCCGAGGGGCGCAACGGCACGCTCGCCGTGATc < 1:560868/111‑1 (MQ=255)
gCCCTGCGCGCGCTGTGGCCGGGCTGGACCGGCTTCGGCATGCAGGACCACACCGTGCTGCTGACCAACGCCTGGACGCCCGAGGGGCGCAACGGCACGCTCGCCGTGATc > 2:560868/1‑111 (MQ=255)
cccTGCGCGCGCTGTGGCCGGGCTGGACCGGCTTCGGCATGCAGGACCACACCGTGCTGCTGACCAACGCCTGGACGCCCGAGGGGCGCAACGGCACGCTCGCCGTGATCAAGGAAGCGGTGCCCACCAGCAA‑g < 2:431576/134‑1 (MQ=255)
cgcTGTGGCCGGGCTGGACCGGCTTCGGCATGCAGGACCACACCGTGCTGCTGACCAACGCCTGGACGCCCGa < 1:241654/73‑1 (MQ=255)
cgcTGTGGCCGGGCTGGACAGGCTTCGGCATGCAGGACCACACCGTGCTGCTGACCAACGCCTGGACGCCCGa > 2:241654/1‑73 (MQ=39)
tgGCCGGGCTGGACCGGCTTCGGCATGCAGGACCACACCGTGCTGCTGACCAACGCCTGGACGCCCGAGGGGCGCAACGGCACGCTCGCCGTGATCAAGGAAGCGGTGCCCACCAGCAAGGGCGGCGTGATCGa > 2:936360/1‑134 (MQ=255)
gggCTGGACCGGCTTCGGCATGCAGGACCACACCGTGCTGCTGACCAACGCCTGGACGCCCGAGGGGCGCAACGGCACGCTCGCCGTGATCAAGGAAGCGGTGCCCACCAGCAAGGGCGGCGTGATCGACACcg > 2:886808/1‑134 (MQ=255)
gCTGGACCGGCTTCGGCATGCAGGACCACACCGTGCTGCTGACCAACGCCTGGACGCCCGAGGGGCGCAACGGCACGCTCGCCGTGATCAAGGAAGCGGTGCCCACCAGCAAGGGCGGCGTGATCGACACcgcg < 1:886808/134‑1 (MQ=255)
cGGCTTCGGCATGCAGGACCACACCGTGCTGCTGACCAACGCCTGGACGCCCGAGGGGCGCAACGGCACGCTCGCCGTGATCAAg < 1:723374/85‑1 (MQ=255)
cGGCTTCGGCATGCAGGACCACACCGTGCTGCTGACCAACGCCTGGACGCCCGAGGGGCGCAACGGCACGCTCGCCGTGATCAAg > 2:723374/1‑85 (MQ=255)
cGGCATGCAGGACCACACCGTGCTGCTGACCAACGCCTGGACGCCCGAGGGGCGCAACGGCACGCTCGCCGTGATCAAGGAAGCGGTGCCCACCAGCAAGGGCGGCGTGATCGACACCGCGCTGACCGTCGGGc > 1:761723/1‑134 (MQ=255)
cATGCAGGACCACACCGTGCTGCTGACCAACGCCTGGACGCCCGAGGGGCGCAACGGCACGCTCGCCGTGATCAAGGAAGCGGTGCCCACCAGCAAGGGCGGCGTGATCGACACCGCGCTGACCGTCGGGCTgg < 1:918298/134‑1 (MQ=255)
gctgACCAACGCCTGGACGCCCGAGGGGCGCAACGGc > 2:417144/1‑37 (MQ=255)
gctgACCAACGCCTGGACGCCCGAGGGGCGCAACGGc < 1:417144/37‑1 (MQ=255)
gctgACCAACGCCTGGACGCCCGAGGGGCGCAACGGCACGCTCGCCGTGATCAAGGAAGCGGTGCCCACCAGCAAGGGCGGCGTGATCGACACcg > 2:208341/1‑95 (MQ=255)
gctgACCAACGCCTGGACGCCCGAGGGGCGCAACGGCACGCTCGCCGTGATCAAGGAAGCGGTGCCCACCAGCAAGGGCGGCGTGATCGACACcg < 1:208341/95‑1 (MQ=255)
|
CGCGCAACCTCTACCGGGCCGAGGTGGGGTTGTCCGTCGCCGGCGACAACGCGGCCCTGCGCGCGCTGTGGCCGGGCTGGACCGGCTTCGGCATGCAGGACCACACCGTGCTGCTGACCAACGCCT‑GACGCCCGAGGGGCGCAACGGCACGCTCGCCGTGATCAAGGAAGCGGTGCCCACCAGCAA‑GGCGGCGTGATCGACACCGCGCTGACCGTCGGGCTGG > AM260479/1071809‑1072031
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
BRESEQ :: bam2aln output
CGACCTGGCCACGCGCAACCTCTACCGGGCCGAGGTGGGGTTGTCCGTCGCCGGCGACAACGCGGCCCTGCGCGCGCTGTGGCCGGGCTGGACCGGCTTCGGCATGCAGGACCACACCGTGCTGCTGACCAACGCCT‑GACGCCCGAGGGGCGCAACGGCACGCTCGCCGTGATCAAGGAAGCGGTGCCCACCAGCAA‑GGCGGCGTGATCGACACCGCGCTGACCGTCGGGCTGGCCGCCTTCGGGCCGGAAATCCTGAAACTGGACCTGGGC > AM260479/1071798‑1072069
|
CGACCTGGCCACGCGCAACCTCTACCGGGCCGAGGTGGGGTTGTCCGTCGCCGGCGACAACGCGGCCCTGCGCGCGCTGTGGCCGGGCTGGACCGGCTTCGGCATGCAGGACCACACCGTGCTGCTGACCAACGCCTGGACGCCCGAGGG > GWNJ‑0478:712:GW2002102894th:2:1107:20465:100813/1‑150 (MQ=60)
ttttttcaagcagaagacggcatacgagatgtagctccgtctcgtgggctcggagatgtgtataagagacaGCGCTGTGGCCGGGCTGGACCGGCTTCGGCATGCAGGACCACACCGTGCTGCTGACCAACGCCTGGACGCCCGAGGGG < GWNJ‑0478:712:GW2002102894th:2:1213:14562:25787/78‑1 (MQ=60)
gatatgtagctccgcctcgtgggctcggagatgtgtataagagacagaccCGGCCCTGCGCGCGCTGTGGCCGGGCTGGACCGGCTTCGGCATGCAGGACCACACCGTGCTGCTGACCAACGCCTGGACGCCCGAGGGGCGCAACGGCAC < GWNJ‑0478:712:GW2002102894th:2:1116:1404:24666/100‑1 (MQ=60)
CTACCGGGCCGAGGTGGGGTTGTCCGTCGCCGGCGACAACGCGGCCCTGCGCGCGCTGTGGCCGGGCTGGACCGGCTTCGGCATGCAGGACCACACCGTGCTGCTGACCAACGCCTGGACGCCCGAGGGGCGCAACGGCACGCTCGCCGT > GWNJ‑0478:712:GW2002102894th:2:1213:5189:33983/1‑150 (MQ=60)
gttttttttttttttttcaagcagaagacggcatacgagatgtagctccgtctcgtgggctcggagatgtgtataagagacagGGACCACACCGTGCTGCTGACCAACGCCTGGACGCCCGAGGGGCGCAACGGCACGCTCGCCGTGATC < GWNJ‑0478:712:GW2002102894th:2:1210:2046:51881/67‑1 (MQ=60)
GGCCGAGGTGGGGTTGTCCGTCGCCGGCGACAACGCGGCCCTGCGCGCGCTGTGGCCGGGCTGGACCGGCTTCGGCATGCAGGACCACACCGTGCTGCTGACCAACGCCTGGACGCCCGAGGGGCGCAACGGCACGCTCGCCGTGATCA > GWNJ‑0478:712:GW2002102894th:2:2214:15338:68956/1‑149 (MQ=60)
CGAGGTGGGGTTGTCCGTCGCCGGCGACAACGCGGCCCTGCGCGCGCTGTGGCCGGGCTGGACCGGCTTCGGCATGCAGGACCACACCGTGCTGCTGACCAACGCCTGGACGCCCGAGGGGCGCAACGGCACGCTCGCCGTGATCAAGGA < GWNJ‑0478:712:GW2002102894th:2:1207:2449:45681/150‑1 (MQ=60)
aagagacagGCCGGCGACAACGCGGCCCTGCGCGCGCTGTGGCCGGGCTGGACCGGCTTCGGCATGCAGGACCACACCGTGCTGCTGACCAACGCCTGGACGCCCGAGGGGCGCAACGGCACGCTCGCCGTGATCAAGGAAGCGGTGCCC < GWNJ‑0478:712:GW2002102894th:2:2105:4864:31392/141‑1 (MQ=60)
cgtctcgtgggctcggagatgtgtataagagacagGTGGCCGGGCTGGACCGGCTTCGGCATGCAGGACCACACCGTGCTGCTGACCAACGCCTGGACGCCCGAGGGGCGCAACGGCACGCTCGCCGTGATCAAGGAAGCGGTGCCCACC < GWNJ‑0478:712:GW2002102894th:2:2115:5074:44406/115‑1 (MQ=60)
GCCGGCGACAACGCGGCCCTGCGCGCGCTGTGGCCGGGCTGGACCGGCTTCGGCATGCAGGACCACACCGTGCTGCTGACCAACGCCT‑Ggacctgtctcttatacacatctccgagcccacgagacggagctacatctcgtatgccgtct > GWNJ‑0478:712:GW2002102894th:2:1211:17185:92575/1‑89 (MQ=60)
gctcggagatgtgtataagagacagGGACCACACCGTGCTGCTGACCAACGCCTGGACGCCCGAGGGGCGCAACGGCACGCTCGCCGTGATCAAGGAAGCGGTGCCCACCAGCAAGGGCGGCGTGATCGACACCGCGCTGACCGTCGGGC < GWNJ‑0478:712:GW2002102894th:2:1115:3319:28640/125‑1 (MQ=60)
GGGCTGGACCGGCTTCGGCATGCAGGACCACACCGTGCTGCTGACCAACGCCTGGACGCCCGAGGGGCGCAACGGCACGCTCGCCGTGATCAAGGAAGCGGTGCCCACCAGCAAGGGCGGCGTGATCGACACCGCGCTGACCGTCGGGCT > GWNJ‑0478:712:GW2002102894th:2:2116:8459:70513/1‑150 (MQ=60)
GGCTGGACCGGCTTCGGCATGCAGGACCACACCGTGCTGCTGACCAACGCCTGGACGCCCGAGGGGCGCAACGGCACGCTCGCCGTGATCAAGGAAGCGGTGCCCACCAGCAAGGGCGGCGTGATCGACACCGCGCTGACCGTCGGGCTG < GWNJ‑0478:712:GW2002102894th:2:2207:17241:84208/150‑1 (MQ=60)
GCATGCAGGACCACACCGTGCTGCTGACCAACGCCTGGACGCCCGAGGGGCGCAACGGCACGCTCGCCGTGATCAAGGAAGCGGTGCCCACCAGCAAGGGCGGCGTGATCGACACCGCGCTGACCGTCGGGCTGGatgcggcccaggtcc < GWNJ‑0478:712:GW2002102894th:2:2211:20466:76637/150‑16 (MQ=60)
GCTGCTGACCAACGCCTGGACGCCCGAGGGGCGCAACGGCACGCTCGCCGTGATCAAGGActgtctcttatacacatctccgagcccacgagacggagctacatctcgtatgccgtcttctgcttgaaaaaaaaaaaaaaaaaaagaaac > GWNJ‑0478:712:GW2002102894th:2:2215:17822:65862/1‑60 (MQ=60)
GACCAACGCCTGGACGCCCGAGGGGCGCAACGGCACGCTCGCCGTGATCAAGGAAGCGGTGCCCACCAGCAAGGGCGGCGTGATCGACACCGCGCTGACCGTCGGGCTGGCCGCCTTCGGGCCGGAAATCCTGAAACTGGACCTGGGC < GWNJ‑0478:712:GW2002102894th:2:2203:1450:14546/148‑1 (MQ=60)
|
CGACCTGGCCACGCGCAACCTCTACCGGGCCGAGGTGGGGTTGTCCGTCGCCGGCGACAACGCGGCCCTGCGCGCGCTGTGGCCGGGCTGGACCGGCTTCGGCATGCAGGACCACACCGTGCTGCTGACCAACGCCT‑GACGCCCGAGGGGCGCAACGGCACGCTCGCCGTGATCAAGGAAGCGGTGCCCACCAGCAA‑GGCGGCGTGATCGACACCGCGCTGACCGTCGGGCTGGCCGCCTTCGGGCCGGAAATCCTGAAACTGGACCTGGGC > AM260479/1071798‑1072069
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
