Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F1 I1 R1
|
150 |
26.0 |
2053212 |
90.0% |
1847890 |
107.8 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
AM260479 |
1,841,200 |
+G |
intergenic (‑2736/‑2680) |
h16_A1684 ← / → ycaC |
conserved hypothetical protein/YcaC related amidohydrolase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | AM260479 | 1,841,197 | 1 | . | G | 100.0%
| 69.5
/ NA
| 19 | intergenic (‑2733/‑2683) | h16_A1684/ycaC | conserved hypothetical protein/YcaC related amidohydrolase |
| Reads supporting (aligned to +/- strand): ref base . (0/0); new base G (8/11); total (8/11) |
CAGCATCCGCCCTCGATGATCCGGCCGGTACGGCAAAGCGCATCGTGGCCGCTGTAGGCAGCCGCCTGCAGGTAGCCATGATAGTCGCGCAGGAAGTTCA‑GGGATGGGCGCCGGCGCGGGATTCCGCGAACTCGAACAGCACCGCTGGCTGGTGGTCGACCCATAATCCGTTCTCCTGCCGCAGCCCGGCCACGAGGTAGCCCCATAGATGGGCCTGCCGCG > AM260479/1841098‑1841319
|
cAGCATCCGCCCTCGATGATCCGGCCGGTACGGCAAAGCGCATCGTGGCCGCTGTAGGCAGCCGCCTGCAGGTAGCCATGATAGTCGCGCAGGAAGTTCAGGGGATGGGCGCCGGCGCGGGATTCCGCGAACTc > 2:778466/1‑134 (MQ=255)
tgatCCGGCCGGTACGGCAAAGCGCATCGTGGCCGCTGTAGGCAGCCGCCTGCAGGTAGCCATGATAGTCGCGCAGGAAGTTCAGGGGATGGGCGCCGGCGCGGGATTCCGCGAACTCGAACAGCACCgctggc > 1:376562/1‑134 (MQ=255)
cggccggTACGGCAAAGCGCATCGTGGCCGCTGTAGGCAGCCGCCTGCAGGTAGCCATGATAGTCGCGCAGGAAGTTCAGGGGATGGGCGCCGGCGCGGGATTCCGCGAACTCGAACAGCACCGCTGGCtggt < 1:509535/133‑1 (MQ=255)
tACGGCAAAGCGCATCGTGGCCGCTGTAGGCAGCCGCCTGCAGGTAGCCATGATAGTCGCGCAGGAAGTTCAGGGGATGGGCGCCGGCGCGGGATTCCGCGAACTCGAACAGCACCGCTGGCTGGTGGTCGAcc < 2:233774/134‑1 (MQ=255)
tCGTGGCCGCTGTAGGCAGCCGCCTGCAGGTAGCCATGATAGTCGCGCAGGAAGTTCAGGGGATGGGCGCCGGCGCGGGATTCCGCGAACTCGAACAGCACCGCTGGCtggtg < 1:276001/113‑1 (MQ=255)
tCGTGGCCGCTGTAGGCAGCCGCCTGCAGGTAGCCATGATAGTCGCGCAGGAAGTTCAGGGGATGGGCGCCGGCGCGGGATTCCGCGAACTCGAACAGCACCGCTGGCtggtg > 2:276001/1‑113 (MQ=255)
gTGGCCGCTGTAGGCAGCCGCCTGCAGGTAGCCATGATAGTCGCACAGGAAGTTCAGGGGATGGGCGCCGGCGCGGGATTCCGCGAACTCGAACAGCACCGCTGGCTGGTGGTCGACCCATAATCCGTTCTCCt < 2:957322/134‑1 (MQ=255)
ccGCTGTAGGCAGCCGCCTGCAGGTAGCCATGATAGTCGCGCAGGAAGTTCAGGGGATGGGCGCCGGCGCGGGATTCCGCGAACTCGAACAGCACCGCTGGCTGGTGGTCGACCCATAATCCGTTCTCCTGCCg < 2:75932/134‑1 (MQ=255)
aGGCAGCCGCCTGCAGGTAGCCATGATAGTCGCGCAGGAAGTTCAGGGGATGGGCGCCGGCGCGGGATTCCGCGAACTCGAACAGCACCGCTGGCTGGTGGTCGACCCATAATCCGTTCTCCTGCCGCAGCCCg > 1:106952/1‑134 (MQ=255)
gccgccTGCAGGTAGCCATGATAGTCGCGCAGGAAGTTCAGGGGATGGGCGCCGGCGCGGGATTCCGCGAACTCGAACAGCACCGCTGGCTGGTGGTCGACCCATAATCCGTTCTCCTGCCGCAGCCCGGCCAc < 2:71881/134‑1 (MQ=255)
gccTGCAGGTAGCCATGATAGTCGCGCAGGAAGTTCAGGGGATGGGCGCCGGCGCGGGATTCCGCGAACTCGAACAGCACCGCTGGCTGGTGGTCGACCCATAATCCGTTCTCCTGCCGCAGCCCGGCCACGAg < 2:106952/134‑1 (MQ=255)
cAGGTAGCCATGATAGTCGCGCAGGAAGTTCAGGGGATGGGCGCCGGCGCGGGATTCCGCGAACTCGAACAGCACCgc < 2:561671/78‑1 (MQ=255)
cAGGTAGCCATGATAGTCGCGCAGGAAGTTCAGGGGATGGGCGCCGGCGCGGGATTCCGCGAACTCGAACAGCACCgc > 1:561671/1‑78 (MQ=255)
cAGGTAGCCATGATAGTCGCGCAGGAAGTTCAGGGGATGGGCGCCGGCGCGGGATTCCGCGAACTCGAACAGCACCGCTGGCTGGTGGTCGACCCATAATCCGTTCTCCTGCCGc > 2:666438/1‑115 (MQ=255)
cAGGTAGCCATGATAGTCGCGCAGGAAGTTCAGGGGATGGGCGCCGGCGCGGGATTCCGCGAACTCGAACAGCACCGCTGGCTGGTGGTCGACCCATAATCCGTTCTCCTGCCGc < 1:666438/115‑1 (MQ=255)
cAGGTAGCCATGATAGTCGCGCAGGAAGTTCAGGGGATGGGCGCCGGCGCGGGATTCCGCGAACTCGAACAGCACCGCTGGCTGGTGGTCGACCCATAATCCGTTCTCCTGCCGCAGCCCGGCCACGAGGTAGc < 1:266895/134‑1 (MQ=255)
cAGGAAGTTCAGGGGATGGGCGCCGGCGCGGGATTCCGCGAACTCGAACAGCACCGCTGGCTGGTGGTCGACCCATAATCCGTTCTCCTGCCGCAGCCCGGCCAc < 2:585485/105‑1 (MQ=255)
cAGGAAGTTCAGGGGATGGGCGCCGGCGCGGGATTCCGCGAACTCGAACAGCACCGCTGGCTGGTGGTCGACCCATAATCCGTTCTCCTGCCGCAGCCCGGCCAc > 1:585485/1‑105 (MQ=255)
cAGGAAGTTCAGGGGATGGGCGCCGGCGCGGGATTCCGCGAACTCGAACAGCACCGCTGGCTGGTGGTCGACCCATAATCCGTTCTCCTGCCGCAGCCCGGCCACGAGGTAGCCCCATAGATGGGCCTGCcgcg > 1:996631/1‑134 (MQ=255)
|
CAGCATCCGCCCTCGATGATCCGGCCGGTACGGCAAAGCGCATCGTGGCCGCTGTAGGCAGCCGCCTGCAGGTAGCCATGATAGTCGCGCAGGAAGTTCA‑GGGATGGGCGCCGGCGCGGGATTCCGCGAACTCGAACAGCACCGCTGGCTGGTGGTCGACCCATAATCCGTTCTCCTGCCGCAGCCCGGCCACGAGGTAGCCCCATAGATGGGCCTGCCGCG > AM260479/1841098‑1841319
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
BRESEQ :: bam2aln output
TCTGGGCCTTGGCGATCTCGAAGAAGCGTCGGCGGCAATGCGCCCAGCATCCGCCCTCGATGATCCGGCCGGTACGGCAAAGCGCATCGTGGCCGCTGTAGGCAGCCGCCTGCAGGTAGCCATGATAGTCGCGCAGGAAGTTCA‑GGGATGGGCGCCGGCGCGGGATTCCGCGAACTCGAACAGCACCGCTGGCTGGTGGTCGACCCATAATCCGTTCTCCTGCCGCAGCCCGGCCACGAGGTAGCCCCATAGATGGGCCTGCCGCGTGGTGGCGCGCCTGCGCTCGAGTAGT > AM260479/1841054‑1841345
|
TCTGGGCCTTGGCGATCTCGAAGAAGCGTCGGCGGCAATGCGCCCAGCATCCGCCCTCGATGATCCGGCCGGTACGGCAAAGCGCATCGTGGCCGCTGTAGGCAGCCGCCTGCAGGTAGCCATGATAGTCGCGCAGGAAGTTCA‑GGGG < GWNJ‑0478:712:GW2002102894th:2:2105:17522:40405/148‑1 (MQ=60)
ttgttttttttttttttttttcaagcagaagacggcatacgagatgtagctccgtctcgtgggctcggagatgtgtataagagacagTGATAGTCGCGCAGGAAGTTCAGGGGATGGGCGCCGGCGCGGGATTCCGCGAACTCGAACAG < GWNJ‑0478:712:GW2002102894th:2:2108:11036:57317/62‑1 (MQ=60)
AGCATCCGCCCTCGATGATCCGGCCGGTACGGCAAAGCGCATCGTGGCCGCTGTAGGCAGCCGCCTGCAGGTAGCCATGATAGTCGCGCAGGAAGTTCAGGGGATGGGCGCCGGCGCGGGATTCCGCGAACTCGAACAGCACCGCTGGCT > GWNJ‑0478:712:GW2002102894th:2:1209:6379:12856/1‑150 (MQ=60)
gagacaGGTACGGCAAAGCGCATCGTGGCCGCTGTAGGCAGCCGCCTGCAGGTAGCCATGATAGTCGCGCAGGAAGTTCAGGGGATGGGCGCCGGCGCGGGATTCCGCGAACTCGAACAGCACCGCTGGCTGGTGGTCGACCCATAATCC < GWNJ‑0478:712:GW2002102894th:2:1203:8294:67149/144‑1 (MQ=60)
CCGGCCGGTACGGCAAAGCGCATCGTGGCCGCTGTAGGCAGCCGCCTGCAGGTAGCCATGATAGTCGCGCAGGAAGTTCAGGGGATGGGCGCCGGCGCGGGATTCCGCGAACTCGAACAGCACCGCTGGCTGGTGGTCGACCCATAATC < GWNJ‑0478:712:GW2002102894th:2:1216:14704:71037/149‑1 (MQ=60)
CATCGTGGCCGCTGTAGGCAGCCGCCTGCAGGTAGCCATGATAGTCGCGCAGGAAGTTCAGGGGATGGGCGCCGGCGCGGGATTCCGCGAACTCGAACAGCACCGCTGGCTGGTGGTCGACCCATAATCCGTTCTCCTGCCGCAGCCCGG > GWNJ‑0478:712:GW2002102894th:2:1108:3497:15404/1‑150 (MQ=60)
gacagGTAGGCAGCCGCCTGCAGGTAGCCATGATAGTCGCGCAGGAAGTTCAGGGGATGGGCGCCGGCGCGGGATTCCGCGAACTCGAACAGCACCGCTGGCTGGTGGTCGACCCATAATCCGTTCTCCTGCCGCAGCCCGGCCACGAGG < GWNJ‑0478:712:GW2002102894th:2:2110:12698:4274/145‑1 (MQ=60)
GTAGGCAGCCGCCTGCAGGTAGCCATGATAGTCGCGCAGGAAGTTCAGGGGATGGGCGCCGGCGCGGGATTCCGCGAACTCGAACAGCACCGCTGGCTGGTGGTCGACCtgtctcttatacacatctccgagcccacgagacggagctac > GWNJ‑0478:712:GW2002102894th:2:2105:17542:36586/1‑109 (MQ=60)
GCAGGTAGCCATGATAGTCGCGCAGGAAGTTCAGGGGATGGGCGCCGGCGCGGGATTCCGCGAACTCGAACAGCACCGCTGGCTGGTGGTCGACCCATAATCCGTTCTCCTGCCGCAGCCCGGCCACGAGGTAGCCCCATAGATGGGCCT < GWNJ‑0478:712:GW2002102894th:2:1203:14740:2379/150‑1 (MQ=60)
GCCATGATAGTCGCGCAGGAAGTTCAGGGGATGGGCGCCGGCGCGGGATTCCGCGAACTCGAACAGCACCGCTGGCTGGTGGTCGACCCATAATCCGTTCTCCTGCCGCAGCCCGGCCACGAGGTAGCCCCATAGctgtctcttatacac > GWNJ‑0478:712:GW2002102894th:2:2104:13883:94665/1‑135 (MQ=60)
GCCATGATAGTCGCGCAGGAAGTTCAGGGGATGGGCGCCGGCGCGGGATTCCGCGAACTCGAACAGCACCGCTGGCTGGTGGTCGACCCATAATCCGTTCTCCTGCCGCAGCCCGGCCACGAGGTAGCCCCATAGATGGGCCTGCCGCGT > GWNJ‑0478:712:GW2002102894th:2:2215:11806:27738/1‑150 (MQ=60)
CAGGAAGTTCAGGGGATGGGCGCCGGCGCGGGATTCCGCGAACTCGAACAGCACCGCTGGCTGGTGGTCGACCCATAATCCGTTCTCCTGCCGCAGCCCGGCCACGAGGTAGCCCCATAGATGGGCCTGCCGCGTGGTGGCGCGCCTGCG > GWNJ‑0478:712:GW2002102894th:2:1214:1455:57574/1‑150 (MQ=60)
GTTCAGGGGATGGGCGCCGGCGCGGGATTCCGCGAACTCGAACAGCACCGCTGGCTGGTGGTCGACCCATAATCCGTTCTCCTGCCGCAGCCCGGCCACGAGGTAGCCCCATAGctgtctcttatacacatctccgagcccacgagacgg > GWNJ‑0478:712:GW2002102894th:2:2202:13087:14238/1‑114 (MQ=60)
G‑GGGATGGGCGCCGGCGCGGGATTCCGCGAACTCGAACAGCACCGCTGGCTGGTGGTCGACCCATAATCCGctgtctcttatacacatctccgagcccacgagacggagctacatctcgtatgccgtcttctgcttgaaaaaaaaaaaa > GWNJ‑0478:712:GW2002102894th:2:1210:9861:28015/1‑71 (MQ=60)
|
TCTGGGCCTTGGCGATCTCGAAGAAGCGTCGGCGGCAATGCGCCCAGCATCCGCCCTCGATGATCCGGCCGGTACGGCAAAGCGCATCGTGGCCGCTGTAGGCAGCCGCCTGCAGGTAGCCATGATAGTCGCGCAGGAAGTTCA‑GGGATGGGCGCCGGCGCGGGATTCCGCGAACTCGAACAGCACCGCTGGCTGGTGGTCGACCCATAATCCGTTCTCCTGCCGCAGCCCGGCCACGAGGTAGCCCCATAGATGGGCCTGCCGCGTGGTGGCGCGCCTGCGCTCGAGTAGT > AM260479/1841054‑1841345
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
