Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F1 I1 R1
|
150 |
26.0 |
2053212 |
90.0% |
1847890 |
107.8 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
AM260479 |
2,616,505 |
C→G |
A45G (GCC→GGC) |
h16_A2404 → |
predicted esterase of the alpha‑beta hydrolase superfamily |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | AM260479 | 2,616,505 | 0 | C | G | 100.0%
| 35.0
/ NA
| 11 | A45G (GCC→GGC) | h16_A2404 | predicted esterase of the alpha‑beta hydrolase superfamily |
| Reads supporting (aligned to +/- strand): ref base C (0/0); new base G (3/8); total (3/8) |
CCTCGGTATCTCCGCGGCCGCCCTGCTGGCGGGCTGCTCGTTCGGCCCGCGTCCCGCGCCGCCGCTCGCCACCACCCCGCCCGCGCCGCGGCCCGTCAAGATCGGCCTGGCGCTGGCCGGCGGCGCGGCGCGCGGCTTTGCCCATATCGGCGTGATCAAGGCGCTCGAAGCGCAAGGCATCCATGCCGACCTGGTCACCGGCACCAGCGCCGGCGCGGTCGTG > AM260479/2616389‑2616611
|
ccTCGGTATCTCCGCGGCCGCCCTGCTGGCGGGCTGCTCGTTCGGCCCGCGTCCCGCGCCGCCGCTCGCCACCACCCCGCCCGCGCCGCGGCCCGTCAAGATCGGCCTGGCGCTGGGCGGCGgcgcggcgcgc < 1:88040/133‑1 (MQ=255)
cgcgGCCGCCCTGCTGGCGGGCTGCTCGTTCGGCCCGCGTCCCGCGCCGCCGCTCGCCACCACCCCGCCCGCGCCGCGGCCCGTCAAGATCGGCCTGGCGCTGGGCGGCGGCGCGGCGCGCGGCTTTGcc < 1:309935/130‑1 (MQ=255)
cGGGCTGCTCGTTCGGCCCGCGTCCCGCGCCGCCGCTCGCCACCACCCCGCCCGCGCCGCGGCCCGTCAAGATCGGCCTGGCGCTGGGCGGCGGCGCGGCGCGCGGCTTTGCCCATATCGGCGTGATCAAGgcg < 2:293997/134‑1 (MQ=255)
tCGTTCGGCCCGCGTCCCGCGCCGCCGCTCGCCACCACCCCGCCCGCGCCGCGGCCCGTCAAGATCGGCCTGGCGCTGGGCGGCGGCGCGGCGCGCGGCTTTGCCCATATCGGCGTGATCAAGGCGCTCGAAgc > 2:148502/1‑134 (MQ=255)
cGTTCGGCCCGCGTCCCGCGCCGCCGCTCGCCACCACCCCGCCCGCGCCGCGGCCCGTCAAGATCGGCCTGGCGCTGGGCGGCGGCGCGGCGCGCGGCTTTGCCCATATCGGCGTGATCAAGGCGCTCGAAgcg < 2:41195/134‑1 (MQ=255)
gcgcCGCCGCTCGCCACCACCCCGCCCGCGCCGCGGCCCGTCAAGATCGGCCTGGCGCTGGGCGGCGGCGCGGCGCGCGGCTTTGCCCATATCGGCGTGATCAAGGCGCTCGAAGCGCAAGGCATCCATGCCGa < 1:148502/134‑1 (MQ=255)
cgcgccgcgGCCCGTCAAGATCGGCCTGGCGCTGGGCGGCGGCGCGGCGCGCGGCTTTGCCCATATCGGCGTGATCAAGGCGCTCGAAGCGCAAGGCATCCATGCCGa < 1:824261/108‑1 (MQ=255)
cgcgccgcgGCCCGTCAAGATCGGCCTGGCGCTGGGCGGCGGCGCGGCGCGCGGCTTTGCCCATATCGGCGTGATCAAGGCGCTCGAAGCGCAAGGCATCCATGCCGa > 2:824261/1‑108 (MQ=255)
ggCCCGTCAAGATCGGCCTGGCGCTGGGCGGCGGCGCGGCGCGCGGCTTTGCCCATATCGGCGTGATCAAGGCGCTCGAAGCGCAAGGCATCCATGCCGACCTGGTCACCGGCACCAGCGCCGGCGCGGTCGTg < 1:188100/134‑1 (MQ=255)
gcgcTGGGCGGCGGCGCGGCGCGCGGCTTTGCCCata > 1:838345/1‑37 (MQ=21)
gcgcTGGGCGGCGGCGCGGCGCGCGGCTTTGCCCata < 2:838345/37‑1 (MQ=21)
|
CCTCGGTATCTCCGCGGCCGCCCTGCTGGCGGGCTGCTCGTTCGGCCCGCGTCCCGCGCCGCCGCTCGCCACCACCCCGCCCGCGCCGCGGCCCGTCAAGATCGGCCTGGCGCTGGCCGGCGGCGCGGCGCGCGGCTTTGCCCATATCGGCGTGATCAAGGCGCTCGAAGCGCAAGGCATCCATGCCGACCTGGTCACCGGCACCAGCGCCGGCGCGGTCGTG > AM260479/2616389‑2616611
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
BRESEQ :: bam2aln output
ACTGATCTCGCTTCATGCAACGACGTCATTTCCTCGGTATCTCCGCGGCCGCCCTGCTGGCGGGCTGCTCGTTCGGCCCGCGTCCCGCGCCGCCGCTCGCCACCACCCCGCCCGCGCCGCGGCCCGTCAAGATCGGCCTGGCGCTGGCCGGCGGCGCGGCGCGCGGCTTTGCCCATATCGGCGTGATCAAGGCGCTCGAAGCGCAAGGCATCCATGCCGACCTGGTCACCGGCACCAGCGCCGGCGCGGTCGTGGCGGCGCTTTACGCCAGCGG > AM260479/2616358‑2616631
|
ACTGATCTCGCTTCATGCAACGACGTCATTTCCTCGGTATCTCCGCGGCCGCCCTGCTGGCGGGCTGCTCGTTCGGCCCGCGTCCCGCGCCGCCGCTCGCCACCACCCCGCCCGCGCCGCGGCCCGTCAAGATCGGCCTGGCGCTGGG < GWNJ‑0478:712:GW2002102894th:2:2211:5752:8759/148‑1 (MQ=60)
TCCTCGGTATCTCCGCGGCCGCCCTGCTGGCGGGCTGCTCGTTCGGCCCGCGTCCCGCGCCGCCGCTCGCCACCACCCCGCCCGCGCCGCGGCCCGTCAAGATCGGCCTGGCGCTGGGCGGCGGCGCGGCGCGCGGCTTTGCCCATATC < GWNJ‑0478:712:GW2002102894th:2:1107:5567:93760/149‑1 (MQ=60)
CCGCGGCCGCCCTGCTGGCGGGCTGCTCGTTCGGCCCGCGTCCCGCGCCGCCGCTCGCCACCACCCCGCCCGCGCCGCGGCCCGTCAAGATCGGCCTGGCGCTGGGCGGCGGCGCGGCGCGCGGCTTTGCCCATATCGGCGTGATC < GWNJ‑0478:712:GW2002102894th:2:1205:3489:90507/146‑1 (MQ=60)
CGCGCCGCCGCTCGCCACCACCCCGCCCGCGCCGCGGCCCGTCAAGATCGGCCTGGCGCTGGGCGGCGGCGCGGCGCGCGGCTTTGCCCATATCGGCGTGATCAAGGCGCTCGAAGCGCAAGGCATCCATGCCGACCTGGTCACCGGCAC < GWNJ‑0478:712:GW2002102894th:2:1111:2531:72084/150‑1 (MQ=60)
ataagagacagCGCCACCACCCCGCCCGCGCCGCGGCCCGTCAAGATCGGCCTGGCGCTGGGCGGCGGCGCGGCGCGCGGCTTTGCCCATATCGGCGTGATCAAGGCGCTCGAAGCGCAAGGCATCCATGCCGACCTGGTCACCGGCAC < GWNJ‑0478:712:GW2002102894th:2:2204:9890:90288/138‑1 (MQ=60)
CGCGCCGCGGCCCGTCAAGATCGGCCTGGCGCTGGGCGGCGGCGCGG < GWNJ‑0478:712:GW2002102894th:2:1205:19139:85798/47‑1 (MQ=60)
CGCGCCGCGGCCCGTCAAGATCGGCCTGGCGCTGGGCGGCGGCGCGG < GWNJ‑0478:712:GW2002102894th:2:2108:11283:81735/47‑1 (MQ=60)
CGGCCCGTCAAGATCGGCCTGGCGCTGGGCGGCGGCGCGGCGCGCGGCTTTGCCCATATCGGCGTGATCAAGGCGCTCGAAGCGCAAGGCATCCATGCCGACCTGGTCACCGGCACCAGCGCCGGCGCGGTCGTGGCGGCGCTTTACGCC < GWNJ‑0478:712:GW2002102894th:2:1112:7159:10357/150‑1 (MQ=60)
GTCAAGATCGGCCTGGCGCTGGGCGGCGGCGCGGCGCGCGGCTTTGCCCATATCGGCGTGATCAAGGCtgtctcttatacacatctccgagcccacgagacggagctacatctcgtatgccgtcttctgcttgaaaaaaaaaaaaaaaa > GWNJ‑0478:712:GW2002102894th:2:2205:15262:82236/1‑68 (MQ=60)
|
ACTGATCTCGCTTCATGCAACGACGTCATTTCCTCGGTATCTCCGCGGCCGCCCTGCTGGCGGGCTGCTCGTTCGGCCCGCGTCCCGCGCCGCCGCTCGCCACCACCCCGCCCGCGCCGCGGCCCGTCAAGATCGGCCTGGCGCTGGCCGGCGGCGCGGCGCGCGGCTTTGCCCATATCGGCGTGATCAAGGCGCTCGAAGCGCAAGGCATCCATGCCGACCTGGTCACCGGCACCAGCGCCGGCGCGGTCGTGGCGGCGCTTTACGCCAGCGG > AM260479/2616358‑2616631
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
