Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F1 I1 R1
|
150 |
26.0 |
2053212 |
90.0% |
1847890 |
107.8 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
AM260479 |
3,739,157 |
C→A |
W319C (TGG→TGT) |
rpoA ← |
DNA‑directed RNA polymerase alpha subunit |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | AM260479 | 3,739,157 | 0 | C | A | 100.0%
| 153.5
/ NA
| 40 | W319C (TGG→TGT) | rpoA | DNA‑directed RNA polymerase alpha subunit |
| Reads supporting (aligned to +/- strand): ref base C (0/0); new base A (17/23); total (17/23) |
CGATGCGGCTGGACGCGGGCCGGTAGTCATACGAGTCCTGGCACGAAGCCAGGGCGGTTACCGCGGTGCGGGACCAATGCCCCGCGCCACAATTACTTCTCCAGACCTGCGGGCGGCCAGTTCTCGAGCTTCATGCCAAGGGTCAGGCCACGCGATGCGAGGACTTCCTTGATCTCGTTGAGCGACTTGCGACCCAGGTTCGGGGTCTTGAGCAGTTCGTTCTCGGTACGCTGGATCAGGTCGC > AM260479/3739041‑3739284
|
cGATGCGGCTGGACGCGGGCCGGTAGTCATACGAGTCCTGGCACGAAGCCAGGGCGGTTACCGCGGTGCGGGACCAATGCCCCGCGCCACAATTACTTCTCCAGACCTGCGGGCGGACAGTTCTCGAGCTTCa < 2:385708/133‑1 (MQ=255)
cGATGCGGCTGGACGCGGGCCGGTAGTCATACGAGTCCTGGCACGAAGCCAGGGCGGTTACCGCGGTGCGGGACCAATGCCCCGCGCCACAATTACTTCTCCAGACCTGCGGGCGGACAGTTCTCGAGCTTCa < 1:121440/133‑1 (MQ=255)
cGATGCGGCTGGACGCGGGCCGGTAGTCATACGAGTCCTGGCACGAAGCCAGGGCGGTTACCGCGGTGCGGGACCAATGCCCCGCGCCACAATTACTTCTCCAGACCTGCGGGCGGACAGTTCTCGAGCTTCa < 2:750966/133‑1 (MQ=255)
cGATGCGGCTGGACGCGGGCCGGTAGTCATACGAGTCCTGGCACGAAGCCAGGGCGGTTACCGCGGTGCGGGACCAATGCCCCGCGCCACAATTACTTCTCCAGACCTGCGGGCGGACAGTTCTCGAGCTTCa < 1:197629/133‑1 (MQ=255)
gATGCGGCTGGACGCGGGCCGGTAGTCATACGAGTCCTGGCACGAAGCCAGGGCGGTTACCGCGGTGCGGGACCAATGCCCCGCGCCACAATTACTTCTCCAGACCTGCGGGCGGACAGTTCTCGAGCTTCATg > 2:642603/1‑134 (MQ=255)
gATGCGGCTGGACGCGGGCCGGTAGTCATACGAGTCCTGGCACGAAGCCAGGGCGGTTACCGCGGTGCGGGACCAATGCCCCGCGCCACAATTACTTCTCCAGACCTGCGGGCGGACAGTTCTCGAGCTTCATg > 2:62689/1‑134 (MQ=255)
aTGCGGCTGGACGCGGGCCGGTAGTCATACGAGTCCTGGCACGAAGCCAGGGCGGTTACCGCGGTGCGGGACCAATGCCCCGCGCCACAATTACTTCTCCAGACCTGCGGGCGGACAGTTCTCGAGCTTCa > 2:759495/1‑131 (MQ=255)
aTGCGGCTGGACGCGGGCCGGTAGTCATACGAGTCCTGGCACGAAGCCAGGGCGGTTACCGCGGTGCGGGACCAATGCCCCGCGCCACAATTACTTCTCCAGACCTGCGGGCGGACAGTTCTCGAGCTTCa < 1:759495/131‑1 (MQ=255)
aTGCGGCTGGACGCGGGCCGGTAGTCATACGAGTCCTGGCACGAAGCCAGGGCGGTTACCGCGGTGCGGGACCAATGCCCCGCGCCACAATTACTTCTCCAGACCTGCGGGCGGACAGTTCTCGAGCTTCATGc > 1:524840/1‑134 (MQ=255)
aTGCGGCTGGACGCGGGCCGGTAGTCATACGAGTCCTGGCACGAAGCCAGGGCGGTTACCGCGGTGCGGGACCAATGCCCCGCGCCACAATTACTTCTCCAGACCTGCGGGCGGACAGTTCTCGAGCTTCATGc > 1:835626/1‑134 (MQ=255)
cGGCTGGACGCGGGCCGGTAGTCATACGAGTCCTGGCACGAAGCCAGGGCGGTTACCGCGGTGCGGGACCAATGCCCCGCGCCACAATTACTTCTCCAGACCTGCGGGCGGACAGTTCTCGAGCTTCATGCCaa < 1:1009115/134‑1 (MQ=255)
ggCTGGACGCGGGCCGGTAGTCATACGAGTCCTGGCACGAAGCCAGGGCGGTTACCGCGGTGCGGGACCAATGCCCCGCGCCACAATTACTCCTCCAGACCTGCGGGCGGACAGTTCTCGAGCTTCATGCCAAg > 2:172317/1‑134 (MQ=255)
gCTGGACGCGGGCCGGTAGTCATACGAGTCCTGGCACGAAGCCAGGGCGGTTACCGCGGTGCGGGACCAATGCCCCGCGCCACAATTACTTCTCCAGACCTGCGGGCGGACAGTTCTCGAGCTTCATGCCAAgg > 1:893670/1‑134 (MQ=255)
gcgGGCCGGTAGTCATACGAGTCCTGGCACGAAGCCAGGGCGGTTACCGCGGTGCGGGACCAATGCCCCGCGCCACAATTACTTCTCCAGACCTGCGGGCGGACAGTTCTCGAGCTTCATGCCAAGGGTCAGGc < 2:927960/134‑1 (MQ=255)
ggTAGTCATACGAGTCCTGGCACGAAGCCAGGGCGGTTACCGCGGTGCGGGACCAATGCCCCGCGCCACAATTACTTCTCCAGACCTGCGGGCGGACAGTTCTCGAGCTTCATGCCAAGGGTCAGGCCACGCGa < 1:998754/134‑1 (MQ=255)
gTCATACGAGTCCTGGCACGAAGCCAGGGCGGTTACCGCGGTGCGGGACCAATGCCCCGCGCCACAATTACTTCTCCAGACCTGCGGGCGGACAGTTCTCGAGCTTCATGCCAAGGGTCAGGCCACGCGATGCg < 2:893670/134‑1 (MQ=255)
cTGGCACGAAGCCAGGGCGGTTACCGCGGTGCGGGACCAATGCCCCGCGCCACAATTACTTCTCCAGACCTGCGGGCGGACAGTTCTCGAGCTTCATGCCAAGGGTCAGGc > 2:189013/1‑111 (MQ=255)
cTGGCACGAAGCCAGGGCGGTTACCGCGGTGCGGGACCAATGCCCCGCGCCACAATTACTTCTCCAGACCTGCGGGCGGACAGTTCTCGAGCTTCATGCCAAGGGTCAGGc < 1:189013/111‑1 (MQ=255)
ggCACGAAGCCAGGGCGGTTACCGCGGTGCGGGACCAATGCCCCGCGCCACAATTACTTCTCCAGACCTGCGGGCGGACAGTTCTCGAGCTTCATGCCAAGGGTCAGGCCACGCGATGCGAGGACTTCCTTGAt > 2:70770/1‑134 (MQ=255)
cACGAAGCCAGGGCGGTTACCGCGGTGCGGGACCAATGCCCCGCGCCACAATTACTTCTCCAGACCTGCGGGCGGACAGTTCTCGAGCTTCATGCCAAGGGTCAGGCCACGCGATGCGAGGACTTCCTTGAtct > 1:973957/1‑134 (MQ=255)
ggTGCGGGACCAATGCCCCGCGCCACAATTACTTCTCCAGACCTGCGGGCGGACAGTTCTCGAGCTTCATGCCAAGGGTCAGGCCACGCGATGCGAGGACTTCCTTGATCTCGTTGAGCGACTTGCGACCCAgg < 1:642603/134‑1 (MQ=255)
ggTGCGGGACCAATGCCCCGCGCCACAATTACTTCTCCAGACCTGCGGGCGGACAGTTCTCGAGCTTCATGCCAAGGGTCAGGCCACGCGATGCGAGGACTTCCTTGATCTCGTTGAGCGACTTGCGACCCAgg < 1:62689/134‑1 (MQ=255)
ggACCAATGCCCCGCGCCACAATTACTTCTCCAGACCTGCGGGCGGACAGTTCTCGAGCTTCATGCCAAGGGTCAGGCCACGCGATGCGAGGACTTCCTTGATCTCGTTGAGCGACTTGCGACCCAGGTTCggg > 1:332268/1‑134 (MQ=255)
cccGCGCCACAATTACTTCTCCAGACCTGCGGGCGGACAGTTCTCGAGCTTCATGCCAAGGGTCAGGCCAcg < 1:181221/72‑1 (MQ=255)
cccGCGCCACAATTACTTCTCCAGACCTGCGGGCGGACAGTTCTCGAGCTTCATGCCAAGGGTCAGGCCAcg > 2:181221/1‑72 (MQ=255)
gcgcCACAATTACTTCTCCAGACCTGCGGGCGGACAGTTCTCGAGCTTCATGCCAAGGGTCAGGCCACGCGATGCGAGGACTTCCTTGATCTCGTTGAGCGACTTGCGACCCAGGTTCGGGGTCTTGAGCAgtt < 1:1016594/134‑1 (MQ=255)
acaATTACTTCTCCAGACCTGCGGGCGGACAGTTCTCGAGCTTCa < 2:336277/45‑1 (MQ=255)
acaATTACTTCTCCAGACCTGCGGGCGGACAGTTCTCGAGCTTCa > 1:336277/1‑45 (MQ=255)
acaATTACTTCTCCAGACCTGCGGGCGGACAGTTCTCGAGCTTCATGCCAAGGGTCAGGCCACGCGATGCGAGGACTTCCTTGATCTCGTTGAGCGACTTGCGACCCAGGTTCGGGGTCTTGAGCAgttcgttc > 2:630355/1‑134 (MQ=255)
aTTACTTCTCCAGACCTGCGGGCGGACAGTTCTCGAGCTTCATGCCAAGGGTCAGGCCACGCGATGCGAGGACTTCCTTGATCTCGTTGAGCGACTTGCGACCCAGGTTCGGGGTCTTGAGCAGTTCGTTCTCg > 1:248309/1‑134 (MQ=255)
ttACTTCTCCAGACCTGCGGGCGGACAGTTCTCGAGCTTCATGCCAAGGGTCAGGCCACGCGATGCGAGGACTTCCTTGATCTCGTTGAGCGACTTGCGACCCAGGTTCGGGGTCTTGAGCAGTTCGTTCTCgg < 2:524840/134‑1 (MQ=255)
ttACTTCTCCAGACCTGCGGGCGGACAGTTCTCGAGCTTCATGCCAAGGGTCAGGCCACGCGATGCGAGGACTTCCTTGATCTCGTTGAGCGACTTGCGACCCAGGTTCGGGGTCTTGAGCAGTTCGTTCTCgg < 1:630355/134‑1 (MQ=255)
ttACTCCTCCAGACCTGCGGGCGGACAGTTCTCGAGCTTCATGCCAAGGGTCAGGCCACGCGATGCGAGGACTTCCTTGATCTCGTTGAGCGACTTGCGACCCAGGTTCGGGGTCTTGAGCAGTTCGTTCTCgg < 1:172317/134‑1 (MQ=255)
aCCTGCGGGCGGACAGTTCTCGAGCTTCATGCCAAGGGTCAGGCCACGCGATGCGAGGACTTCCTTGATCTCGTTg < 2:357508/76‑1 (MQ=255)
aCCTGCGGGCGGACAGTTCTCGAGCTTCATGCCAAGGGTCAGGCCACGCGATGCGAGGACTTCCTTGATCTCGTTg > 1:357508/1‑76 (MQ=255)
cTGCGGGCGGACAGTTCTCGAGCTTCATGCCAAGGGTCAGGCCACGCGATGCGAGGACTTCCTTGATCTCGTTGAGCGACTTGCGACCCAGGTTCGGGGTCTTGAGCAGTTCGTTCTCGGTACGCTGGATCAgg < 2:973957/134‑1 (MQ=255)
cgggcggACAGTTCTCGAGCTTCATGCCAAGGGTCAGGCCACGCGATGCGAGGACTTCCTTGATCTCGTTGAGCGACTTGCGACCCAGGTTCGGGGTCTTGAGCAGTTCGTTCTCGGTACGCTGGATCAGGTCg < 2:248309/134‑1 (MQ=255)
gggcggACAGTTCTCGAGCTTCATGCCAAGGGTCAGGCCACGCGATGCGAGGACTTCCTTGATCTCGTTGAGCGACTTGCGACCCAGGTTCGGGGTCt > 2:561657/1‑98 (MQ=255)
gggcggACAGTTCTCGAGCTTCATGCCAAGGGTCAGGCCACGCGATGCGAGGACTTCCTTGATCTCGTTGAGCGACTTGCGACCCAGGTTCGGGGTCt < 1:561657/98‑1 (MQ=255)
gggcggACAGTTCTCGAGCTTCATGCCAAGGGTCAGGCCACGCGATGCGAGGACTTCCTTGATCTCGTTGAGCGACTTGCGACCCAGGTTCGGGGTCTTGAGCAGTTCGTTCTCGGTACGCTGGATCAGGTCGc < 2:835626/134‑1 (MQ=255)
cggaCAGTTCTCGAGCTTCATGCCAAGGGTCAGGCCACGCGATGCGAGGACTTCCTTGATCTCGTTGAGCGACTTGCGACCCAGGTTCgg < 1:431677/90‑1 (MQ=255)
|
CGATGCGGCTGGACGCGGGCCGGTAGTCATACGAGTCCTGGCACGAAGCCAGGGCGGTTACCGCGGTGCGGGACCAATGCCCCGCGCCACAATTACTTCTCCAGACCTGCGGGCGGCCAGTTCTCGAGCTTCATGCCAAGGGTCAGGCCACGCGATGCGAGGACTTCCTTGATCTCGTTGAGCGACTTGCGACCCAGGTTCGGGGTCTTGAGCAGTTCGTTCTCGGTACGCTGGATCAGGTCGC > AM260479/3739041‑3739284
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
BRESEQ :: bam2aln output
CAGTTAAGTGTTTGACCAGCTCTTCTATCGTCCGATGCGGCTGGACGCGGGCCGGTAGTCATACGAGTCCTGGCACGAAGCCAGGGCGGTTACCGCGGTGCGGGACCAATGCCCCGCGCCACAATTACTTCTCCAGACCTGCGGGCGGCCAGTTCTCGAGCTTCATGCCAAGGGTCAGGCCACGCGATGCGAGGACTTCCTTGATCTCGTTGAGCGACTTGCGACCCAGGTTCGGGGTCTTGAGCAGTTCGTTCTCGGTACGCTGGATCAGGTCGCCGA > AM260479/3739009‑3739287
|
CAGTTAAGTGTTTGACCAGCTCTTCTATCGTCCGATGCGGCTGGACGCGGGCCGGTAGTCATACGAGTCCTGGCACGAAGCCAGGGCGGTTACCGCGGTGCGGGACCAATGCCCCGCGCCACAATTACTTCTCCAGACCTGCGGGCGGAC > GWNJ‑0478:712:GW2002102894th:2:2116:20544:4258/1‑150 (MQ=60)
CTCTTCTATCGTCCGATGCGGCTGGACGCGGGCCGGTAGTCATACGAGTCCTGGCACGAAGCCAGGGCGGTTACCGCGGTGCGGGACCAATGCCCCGCGCCACAATTACTTCTCCAGACCTGCGGGCGGACAGTTCTCGAGCTTCATGCC > GWNJ‑0478:712:GW2002102894th:2:2205:20789:64438/1‑150 (MQ=60)
CTCTTCTATCGTCCGATGCGGCTGGACGCGGGCCGGTAGTCATACGAGTCCTGGCACGAAGCCAGGGCGGTTACCGCGGTGCGGGACCAATGCCCCGCGCCACAATTACTTCTCCAGACCTGCGGGCGGACAGTTCTCGAGCTTCATGCC > GWNJ‑0478:712:GW2002102894th:2:2101:9300:78106/1‑150 (MQ=60)
CTATCGTCCGATGCGGCTGGACGCGGGCCGGTAGTCATACGAGTCCTGGCACGAAGCCAGGGCGGTTACCGCGGTGCGGGACCAATGCCCCGCGCCACAATTACTTCTCCAGACCTGCGGGCGGACAGTTCTCGAGCTTCATGCCAAGGG > GWNJ‑0478:712:GW2002102894th:2:2208:11472:28753/1‑150 (MQ=60)
gtgtgtttcctttttttttttttttcaagcagaagacggcatacgagatgtagctccgtctcgtgggctcggagatgtgtataagagacagCAATTACTTCTCCAGACCTGCGGGCGGACAGTTCTCGAGCTTCATGCCAAGGGTCAGGC < GWNJ‑0478:712:GW2002102894th:2:2212:11765:40506/59‑1 (MQ=60)
CCGATGCGGCTGGACGCGGGCCGGTAGTCATACGAGTCCTGGCACGAAGCCAGGGCGGTTACCGCGGTGCGGGACCAATGCCCCGCGCCACAATTACTTCTCCAGACCTGCGGGCGGACAGTTCTCGAGCTTCATGCCAAGGGTCAGGC < GWNJ‑0478:712:GW2002102894th:2:1109:15805:6586/149‑1 (MQ=60)
CCGATGCGGCTGGACGCGGGCCGGTAGTCATACGAGTCCTGGCACGAAGCCAGGGCGGTTACCGCGGTGCGGGACCAATGCCCCGCGCCACAATTACTTCTCCAGACCTGCGGGCGGACAGTTCTCGAGCTTCATGCCAAGGGTCAGGC < GWNJ‑0478:712:GW2002102894th:2:2116:2704:57068/149‑1 (MQ=60)
CCGATGCGGCTGGACGCGGGCCGGTAGTCATACGAGTCCTGGCACGAAGCCAGGGCGGTTACCGCGGTGCGGGACCAATGCCCCGCGCCACAATTACTTCTCCAGACCTGCGGGCGGACAGTTCTCGAGCTTCATGCCAAGGGTCAGGC < GWNJ‑0478:712:GW2002102894th:2:1112:15990:66490/149‑1 (MQ=60)
GCGGCTGGACGCGGGCCGGTAGTCATACGAGTCCTGGCACGAAGCCAGGGCGGTTACCGCGGTGCGGGACCAATGCCCCGCGCCACAATTACTTCTCCAGACCTGCGGGCGGACAGTTCTCGAGCTTCATGCCAAGGGTCAGGCCACGCG < GWNJ‑0478:712:GW2002102894th:2:2215:19251:99468/150‑1 (MQ=60)
aagagacaGGTAGTCATACGAGTCCTGGCACGAAGCCAGGGCGGTTACCGCGGTGCGGGACCAATGCCCCGCGCCACAATTACTTCTCCAGACCTGCGGGCGGACAGTTCTCGAGCTTCATGCCAAGGGTCAGGCCACGCGATGCGAGGA < GWNJ‑0478:712:GW2002102894th:2:1112:6420:15886/142‑1 (MQ=60)
cgagatgtagctccgtctcgtgggctcggagatgtgtataagagacaGGTGCGGGACCAATGCCCCGCGCCACAATTACTTCTCCAGACCTGCGGGCGGACAGTTCTCGAGCTTCATGCCAAGGGTCAGGCCACGCGATGCGAGGACTTC < GWNJ‑0478:712:GW2002102894th:2:1113:9660:68009/103‑1 (MQ=60)
CGGTAGTCATACGAGTCCTGGCACGAAGCCAGGGCGGTTACCGCGGTGCGGGACCAATGCCCCGCGCCACAATTACTTCTCCAGACCTGCGGGCGGACAGTTCTCGAGCTTCATGCCAAGGGTCAGGCCACGCGATGCGAGGACTTCCTT < GWNJ‑0478:712:GW2002102894th:2:2215:16208:38862/150‑1 (MQ=60)
TCATACGAGTCCTGGCACGAAGCCAGGGCGGTTACCGCGGTGCGGGACCAATGCCCCGCGCCACAATTACTTCTCCAGACCTGCGGGCGGACAGTTCTCGAGCTTCATGCCAAGGGTCAGGCCACGCGATGCGAGGACTTCCTTGATCTC > GWNJ‑0478:712:GW2002102894th:2:2213:14056:100028/1‑150 (MQ=60)
GGTTACCGCGGTGCGGGACCAATGCCCCGCGCCACAATTACTTCTCCAGACCTGCGGGCGGACAGTTCTCGAGCTTCATGCCAAGGGTCAGGCCACGCGATGCGAGGACTTCCTTGATCTCGTTGAGCGACTTGCGACCCAGGTTCGGGG > GWNJ‑0478:712:GW2002102894th:2:1207:18475:37473/1‑150 (MQ=60)
CGGTGCGGGACCAATGCCCCGCGCCACAATTACTTCTCCAGACCTGCGGGCGGACAGTTCTCGAGCTTCATGCCAAGGGTCAGGCCACGCGATGCGAGGACTTCCTTGATCTCGTTGAGCGACTTGCGACCCAGGTTCGGGGTCTTGAGC < GWNJ‑0478:712:GW2002102894th:2:2109:13202:63011/150‑1 (MQ=60)
CGGTGCGGGACCAATGCCCCGCGCCACAATTACTTCTCCAGACCTGCGGGCGGACAGTTCTCGAGCTTCATGCCAAGGGTCAGGCCACGCGATGCGAGGACTTCCTTGATCTCGTTGAGCGACTTGCGACCCAGGTTCGGGGTCTTGAGC < GWNJ‑0478:712:GW2002102894th:2:1104:16629:28803/150‑1 (MQ=60)
cgtgggctcggagatgtgtataagagacagCTCCAGACCTGCGGGCGGACAGTTCTCGAGCTTCATGCCAAGGGTCAGGCCACGCGATGCGAGGACTTCCTTGATCTCGTTGAGCGACTTGCGACCCAGGTTCGGGGTCTTGAGCAGTTC < GWNJ‑0478:712:GW2002102894th:2:1213:20807:34240/120‑1 (MQ=60)
cggagatgtgtataagagacagCTTCTCCAGACCTGCGGGCGGACAGTTCTCGAGCTTCATGCCAAGGGTCAGGCCACGCGATGCGAGGACTTCCTTGATCTCGTTGAGCGACTTGCGACCCAGGTTCGGGGTCTTGAGCAGTTCGTTCT < GWNJ‑0478:712:GW2002102894th:2:2105:9876:36739/128‑1 (MQ=60)
CCAATGCCCCGCGCCACAATTACTTCTCCAGACCTGCGGGCGGACAGTTCTCGAGCTTCATGCCAAGGGTCAGGCCtgtctcttatacacatctccgagcccacgagacggagctacatctcgtatgccgtcttctgcttgaaaaaaaa > GWNJ‑0478:712:GW2002102894th:2:1207:14679:61925/1‑76 (MQ=60)
ATGCCCCGCGCCACAATTACTTCTCCAGACCTGCGGGCGGACAGTTCTCGAGCTTCATGCCAAGGGTCAGGCCACGCGATGCGAGGACTTCCTTGATCTCGTTGAGCGACTTGCGACCCAGGTTCGGGGTCTTGAGCAGTTCGTTCTCGG > GWNJ‑0478:712:GW2002102894th:2:1116:6304:68393/1‑150 (MQ=60)
CGCGCCACAATTACTTCTCCAGACCTGCGGGCGGACAGTTCTCGAGCTTCATGCCAAGGGTCAGGCCACGCGATGCGAGGACTTCCTTGATCTCGTTGAGCGACTTGCGACCCAGGTTCGGGGTCTTGAGCAGTTCGTTCTCGGTACGCT < GWNJ‑0478:712:GW2002102894th:2:2216:4213:42045/150‑1 (MQ=60)
CAATTACTTCTCCAGACCTGCGGGCGGACAGTTCTCGAGCTTCATGCCAAGGGTCAGGCCACGCGATGCGAGGACTTCCTTGATCTCGTTGAGCGACTTGCGACCCctgtctcttatacacatctccgagcccacgagacggagctacat > GWNJ‑0478:712:GW2002102894th:2:1206:5102:95824/1‑106 (MQ=60)
gagacagCTCCAGACCTGCGGGCGGACAGTTCTCGAGCTTCATGCCAAGGGTCAGGCCACGCGATGCGAGGACTTCCTTGATCTCGTTGAGCGACTTGCGACCCAGGTTCGGGGTCTTGAGCAGTTCGTTCTCGGTACGCTGGATCAGGT < GWNJ‑0478:712:GW2002102894th:2:2204:11460:75999/143‑1 (MQ=60)
ATTACTTCTCCAGACCTGCGGGCGGACAGTTCTCGAGCTTCATGCCAAGGGTCAGGCCACGCGATGCGAGGACTTCCTTGATCTCGTTGAGCGACTTGCGACCCAGGTTCGGGGTCTTGAGCAGTTCGTTCTCGGTACGCTGGATCAGGT < GWNJ‑0478:712:GW2002102894th:2:2108:11019:86397/150‑1 (MQ=60)
ATTACTCCTCCAGACCTGCGGGCGGACAGTTCTCGAGCTTCATGCCAAGGGTCAGGCCACGCGATGCGAGGACTTCCTTGATCTCGTTGAGCGACTTGCGACCCAGGTTCGGGGTCTTGAGCAGTTCGTTCTCGGTACGCTGGATCAGGT < GWNJ‑0478:712:GW2002102894th:2:1113:15198:15655/150‑1 (MQ=60)
CTCCAGACCTGCGGGCGGACAGTTCTCGAGCTTCATGCCAAGGGctgtctcttatacacatctccgagcccacgagacggagctacatctcgtatgccgtcttctgcttgaaaaaaaaaaaaaaaaaagaaacaagaaaaatcaagaca > GWNJ‑0478:712:GW2002102894th:2:1201:9552:10763/1‑44 (MQ=60)
CTCCAGACCTGCGGGCGGACAGTTCTCGAGCTTCATGCCAAGGGTCAGGCCACGCGATGCGAGGACTgtctcttatacacatctccgagcccacgagacggagctacatctcgtatgccgtcttctgcttgaaaaaaaaaaaaaaaaa > GWNJ‑0478:712:GW2002102894th:2:1202:8061:73971/1‑67 (MQ=60)
|
CAGTTAAGTGTTTGACCAGCTCTTCTATCGTCCGATGCGGCTGGACGCGGGCCGGTAGTCATACGAGTCCTGGCACGAAGCCAGGGCGGTTACCGCGGTGCGGGACCAATGCCCCGCGCCACAATTACTTCTCCAGACCTGCGGGCGGCCAGTTCTCGAGCTTCATGCCAAGGGTCAGGCCACGCGATGCGAGGACTTCCTTGATCTCGTTGAGCGACTTGCGACCCAGGTTCGGGGTCTTGAGCAGTTCGTTCTCGGTACGCTGGATCAGGTCGCCGA > AM260479/3739009‑3739287
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
