Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F1 I1 R1
|
150 |
26.0 |
2053212 |
90.0% |
1847890 |
107.8 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
AM260480 |
1,192,562 |
+C |
coding (163/594 nt) |
cccA → |
cytochrome c, mono‑or diheme variant |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | AM260480 | 1,192,560 | 1 | . | C | 100.0%
| 82.1
/ NA
| 22 | V54A (GTC→GCC) | cccA | cytochrome c, mono‑or diheme variant |
| Reads supporting (aligned to +/- strand): ref base . (0/0); new base C (9/13); total (9/13) |
CGGGCCTGGTCCGGCTGGCTGGTATGGCTCTTGCGGAAACAGGGCAAACAACCAAGACCCGTCCACTGTAGGAGACATCCATGAAAACGCAGCGCCGCCTGCTTGCCTTGCTCGCGCTTTGCGCGT‑CCTGACCGCCCTCCCGGCCGGCTCCGTGCTGGCCCATGGCGACGTCACGCCCCAGGCCGTCGATACCAAAGCGTTACCACAACTGGGCGACGAATGGCGCCCGGACAATCCCTACCGTAGCGG > AM260480/1192435‑1192683
|
cGGGCCTGGTCCGGCTGGCTGGTATGGCTCTTGCGGAAACAGGGCAAACAACCAAGACCCGTCCACTGTAGGAGACATCCATGAAAACGCAGCGCCGCCTGCTTGCCTTGCTCGCGCTTTGCGCGTCCCTGAcc > 1:103585/1‑134 (MQ=255)
ggCCTGGTCCGGCTGGCTGGTATGGCTCTTGCGGAAACAGGGCAAACAACCAAGACCCGTCCACTGTAGGAGACATCCATGAAAACGCAGCGCCGCCTGCTTGCCTTGCTCGCGCTTTGCGCGTCCCTGACCGc < 2:66996/134‑1 (MQ=255)
gctggTATGGCTCTTGCGGAAACAGGGCAAACAACCAAGACCCGTCCACTGTAGGAGACATCCATGAAAACGCAGCGCCGCCTGCTTGCCTTGCTCGCGCTTTGCGCGTCCCTGACCGCCCTCCCGGCCGGCTc < 2:1002091/134‑1 (MQ=255)
gCGGAAACAGGGCAAACAACCAAGACCCGTCCACTGTAGGAGACATCCATGAAAACGCAGCGCCGCCTGCTTGCCTTGCTCGCGCTTTGCGCGTCCCTGACCGCCCTCCCGGCCGGCTCCGTGCTGGCCCAt < 2:88098/132‑1 (MQ=255)
aacaacCAAGACCCGTCCACTGTAGGAGACATCCATGAAAACGCAGCGCCGCCTGCTTGCCTTGCTCGCGCTTTGCGCGTCCCTGACCGCCCTCCCGGCCGGCTCCGTGCTGGCCCATGGCGACGTCACGccc > 1:651407/1‑133 (MQ=255)
aacaacCAAGACCCGTCCACTGTAGGAGACATCCATGAAAACGCAGCGCCGCCTGCTTGCCTTGCTCGCGCTTTGCGCGT‑ccctg < 2:935602/85‑4 (MQ=255)
aacaacCAAGACCCGTCCACTGTAGGAGACATCCATGAAAACGCAGCGCCGCCTGCTTGCCTTGCTCGCGCTTTGCGCGT‑ccctg > 1:935602/1‑82 (MQ=255)
acaacCAAGACCCGTCCACTGTAGGAGACATCCATGAAAACGCAGCGCCGCCTGCTTGCCTTGCTCGCGCTTTGCGCGTCCCTGACCGCCCTCCCGGCCGGCTCCGTGCTGGCCCATGGCGACGTCACGccc > 2:251566/1‑132 (MQ=255)
aCCCGTCCACTGTAGGAGACATCCATGAAAACGCAGCGCCGCCTGCTTGCCTTGCTCGCGCTTTGCGCGTCCCTGACCGCCCTCCCGGCCGGCTCCGTGCTGGCCCATGGCGACGTCACGCCCCAGGcc > 2:79779/1‑129 (MQ=255)
aCCCGTCCACTGTAGGAGACATCCATGAAAACGCAGCGCCGCCTGCTTGCCTTGCTCGCGCTTTGCGCGTCCCTGACCGCCCTCCCGGCCGGCTCCGTGCTGGCCCATGGCGACGTCACGCCCCAGGcc < 1:79779/129‑1 (MQ=255)
tCCACTGTAGGAGACATCCATGAAAACGCAGCGCCGCCTGCTTGCCTTGCTCGCGCTTTGCGCGTCCCTGACCGCCCTCCCGGCCGGCTCCGTGCTGGCCCATGGCGACGTCACGCCCCAGGCCGTCGATACCa < 2:103585/134‑1 (MQ=255)
tCCACTGTAGGAGACATCCATGAAAACGCAGCGCCGCCTGCTTGCCTTGCTCGCGCTTTGCGCGTCCCTGACCGCCCTCCCGGCCGGCTCCGTGCTGGCCCATGGCGACGTCACGCCCCAGGCCGTCGATACCa < 1:251566/134‑1 (MQ=255)
cACTGTAGGAGACATCCATGAAAACGCAGCGCCGCCTGCTTGCCTTGCTCGCGCTTTGCGCGTCCCTGACCGCCCTCCCGGCCGGCTCCGTGCTGGCCCATGGCGACGTCACGCCCCAGGCCGTCGATACCaaa < 2:389825/134‑1 (MQ=255)
cATCCATGAAAACGCAGCGCCGCCTGCTTGCCTTGCTCGCGCTTTGCGCGTCCCTGACCGCCCTCCCGGCCGGCTCCGTGCTGGCCCATGGCGACGTCACGCCCCAGGCCGTCGATACCAAAGCGTTACCACaa < 2:651407/134‑1 (MQ=255)
gAAAACGCAGCGCCGCCTGCTTGCCTTGCTCGCGCTTTGCGCGTCCCTGACCGCCCTCccggcc < 2:152309/64‑1 (MQ=255)
gAAAACGCAGCGCCGCCTGCTTGCCTTGCTCGCGCTTTGCGCGTCCCTGACCGCCCTCccggcc > 1:152309/1‑64 (MQ=255)
gAAAACGCAGCGCCGCCTGCTTGCCTTGCTCGCGCTTTGCGCGTCCCTGACCGCCCTCCCGGCCGGCTCCGTGCTGGCCCAt < 1:729975/82‑1 (MQ=255)
gAAAACGCAGCGCCGCCTGCTTGCCTTGCTCGCGCTTTGCGCGTCCCTGACCGCCCTCCCGGCCGGCTCCGTGCTGGCCCAt > 2:729975/1‑82 (MQ=255)
gAAAACGCAGCGCCGCCTGCTTGCCTTGCTCGCGCTTTGCGCGTCCCTGACCGCCCTCCCGGCCGGCTCCGTGCTGGCCCATGGCGACGTCACGccc > 1:408073/1‑97 (MQ=255)
gAAAACGCAGCGCCGCCTGCTTGCCTTGCTCGCGCTTTGCGCGTCCCTGACCGCCCTCCCGGCCGGCTCCGTGCTGGCCCATGGCGACGTCACGccc < 2:408073/97‑1 (MQ=255)
aaCGCAGCGCCGCCTGCTTGCCTTGCTCGCGCTTTGCGCGTCCCTGACCGCCCTCCCGGCCGGCTCCGTGCTGGCCCATGGCGACGTCACGCCCCAGGCCGTCGATACCAAAGCGTTACCACAACTGGGcgac > 1:402456/1‑133 (MQ=255)
ccgccTGCTTGCCTTGCTCGCGCTTTGCGCGTCCCTGACCGCCCTCCCGGCCGGCTCCGTGCTGGCCCATGGCGACGTCACGCCCCAGGCCGTCGATACCAAAGCGTTACCACAACTGGGCGACGAATGGCGcc > 2:77999/1‑134 (MQ=255)
cttgccttgcTCGCGCTTTGCGCGTCCCTGACCGCCCTCCCGGCCGGCTCCGTGCTGGCCCATGGCGACGTCACGCCCCAGGCCGTCGATACCAAAGCGTTACCACAACTGGGCGACGAATGGCGCCCGGACaa < 1:77999/134‑1 (MQ=255)
cTTTGCGCGTCCCTGACCGCCCTCCCGGCCGGCTCCGTGCTGGCCCATGGCGACGTCACGCCCCAGGCCGTCGATACCAAAGCGTTACCACAACTGGGCGACGAATGGCGCCCGGACAATCCCTACCGTAGCgg < 1:29873/134‑1 (MQ=255)
|
CGGGCCTGGTCCGGCTGGCTGGTATGGCTCTTGCGGAAACAGGGCAAACAACCAAGACCCGTCCACTGTAGGAGACATCCATGAAAACGCAGCGCCGCCTGCTTGCCTTGCTCGCGCTTTGCGCGT‑CCTGACCGCCCTCCCGGCCGGCTCCGTGCTGGCCCATGGCGACGTCACGCCCCAGGCCGTCGATACCAAAGCGTTACCACAACTGGGCGACGAATGGCGCCCGGACAATCCCTACCGTAGCGG > AM260480/1192435‑1192683
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
BRESEQ :: bam2aln output
GCTTTCAGGCGCCTCCGGGCCTGGTCCGGCTGGCTGGTATGGCTCTTGCGGAAACAGGGCAAACAACCAAGACCCGTCCACTGTAGGAGACATCCATGAAAACGCAGCGCCGCCTGCTTGCCTTGCTCGCGCTTTGCGCGT‑CCTGACCGCCCTCCCGGCCGGCTCCGTGCTGGCCCATGGCGACGTCACGCCCCAGGCCGTCGATACCAAAGCGTTACCACAACTGGGCGACGAATGGCGCCCGGACAATCCCTACCGTAGCGGTGATGCGCACAAGGAGGCTCTGCGG > AM260480/1192420‑1192708
|
GCTTTCAGGCGCCTCCGGGCCTGGTCCGGCTGGCTGGTATGGCTCTTGCGGAAACAGGGCAAACAACCAAGACCCGTCCACTGTAGGAGACATCCATGAAAACGCAGCGCCGCCTGCTTGCCTTGCTCGCGCTTTGCGCGTCCCTGACCG > GWNJ‑0478:712:GW2002102894th:2:1106:13461:93742/1‑150 (MQ=60)
ttcaagcagaagacggcatacgagatgtagctccgtctcgtgggctcggagatgtgtataagagacagCTGTAGGAGACATCCATGAAAACGCAGCGCCGCCTGCTTGCCTTGCTCGCGCTTTGCGCGTCCCTGACCGCCCTCCCGGCC < GWNJ‑0478:712:GW2002102894th:2:1105:11793:59722/81‑1 (MQ=60)
ccgtctcgtgggctcggagatgtgtataagagacagGTAGGAGACATCCATGAAAACGCAGCGCCGCCTGCTTGCCTTGCTCGCGCTTTGCGCGTCCCTGACCGCCCTCCCGGCCGGCTCCGTGCTGGCCCATGGCGACGTCACGCCC < GWNJ‑0478:712:GW2002102894th:2:2115:4637:81203/112‑1 (MQ=60)
TGCGGAAACAGGGCAAACAACCAAGACCCGTCCACTGTAGGAGACATCCATGAAAACGCAGCGCCGCCTGCTTGCCTTGCTCGCGCTTTGCGCGTCCCTGACCGCCCTCCCGGCCctgtctcttatacacatctccgagcccacgagacg > GWNJ‑0478:712:GW2002102894th:2:2210:12270:78884/1‑115 (MQ=60)
TGCGGAAACAGGGCAAACAACCAAGACCCGTCCACTGTAGGAGACATCCATGAAAACGCAGCGCCGCCTGCTTGCCTTGCTCGCGCTTTGCGCGTCCCTGACCGCCCTCCCGGCCGGCTCCGTGCTGGCCCATGGCGACGTCACGCCCC > GWNJ‑0478:712:GW2002102894th:2:2111:17888:15961/1‑149 (MQ=60)
ACCAAGACCCGTCCACTGTAGGAGACATCCATGAAAACGCAGCGCCGCCTGCTTGCCTTGCTCGCGCTTTGCGCGTCCCTGACCGCCCTCCCGGCCGGCTCCGTGCTGGCCCATGGCGACGTCACGCCCCAGGCCGTCGATACCAAAGCG < GWNJ‑0478:712:GW2002102894th:2:1107:9393:41404/150‑1 (MQ=60)
GTCCACTGTAGGAGACATCCATGAAAACGCAGCGCCGCCTGCTTGCCTTGCTCGCGCTTTGCGCGTCCCTGACCGCCCTCCCGGCCGGCTCCGTGCTGGCCCATGGCGACGTCACGCCCCAGGCCGTCGATACCAAAGCGTTACCACAAC < GWNJ‑0478:712:GW2002102894th:2:1116:9206:93693/150‑1 (MQ=60)
GTAGGAGACATCCATGAAAACGCAGCGCCGCCTGCTTGCCTTGCTCGCGCTTTGCGCGTCCCTGACCGCCCTCCCGGCCGGCTCCGTGCTGGCCCtgtctcttatacacatctccgagcccacgagacggagctacatctcgtatgccgt > GWNJ‑0478:712:GW2002102894th:2:1111:2152:93967/1‑95 (MQ=60)
GTAGGAGACATCCATGAAAACGCAGCGCCGCCTGCTTGCCTTGCTCGCGCTTTGCGCGTCCCTGACCGCCCTCCCGGCCGGCTCCGTGCTGGCCCATGGCGACGTCACGCCCCAGGCCGTCGATACCctgtctcttatacacatctccg > GWNJ‑0478:712:GW2002102894th:2:1211:4717:99278/1‑127 (MQ=60)
GGAGACATCCATGAAAACGCAGCGCCGCCTGCTTGCCTTGCTCGCGCTTTGCGCGTCCCTGACCGCCCTCCCGGCCGGCTCCGTGCTGGCCCATGGCGACGTCACGCCCCAGGCCGTCGATACCAAAGCGTTACCACAACTGGGCGACG > GWNJ‑0478:712:GW2002102894th:2:1211:6961:66556/1‑149 (MQ=60)
GCTTGCCTTGCTCGCGCTTTGCGCGTCCCTGACCGCCCTCCCGGCCGGCTCCGTGCTGGCCCATGGCGACGTCACGCCCCAGGCCGTCGATACCAAAGCGTTACCACAACTGGGCGACGAATGGCGCCCGGACAATCCCTACCGTAGCGG < GWNJ‑0478:712:GW2002102894th:2:1107:20168:30064/150‑1 (MQ=60)
TGCTCGCGCTTTGCGCGTCCCTGACCGCCCTCCCGGCCGGCTCCGTGCTGGCCCATGGCGACGTCACGCCCCAGGCCGTCGATACCAAAGCGTTACCACAACTGGGCGACGAATGGCGCCCGGACAATCCCTACCGTAGCGGTGATGCGC > GWNJ‑0478:712:GW2002102894th:2:1215:9437:100169/1‑150 (MQ=60)
GCTTTGCGCGTCCCTGACCGCCCTCCCGGCCGGCTCCGTGCTGGCCCATGGCGACGTCACGCCCCAGGCCGTCGATACCAAAGCGTTACCACAACTGGGCGACGAATGGCGCCCGGACAATCCCTACCGTAGCGGTGATGCGCACAAGGA < GWNJ‑0478:712:GW2002102894th:2:1105:8913:7034/150‑1 (MQ=60)
tcCCTGACCGCCCTCCCGGCCGGCTCCGTGCTGGCCCATGGCGACGTCACGCCCCAGGCCGTCGATACCAAAGCGTTACCACAACTGGGCGACGAATctgtctcttatacacatctccgagcccacgagacggagctacatctcgtatgc > GWNJ‑0478:712:GW2002102894th:2:2209:17891:32623/3‑97 (MQ=60)
tcCCTGACCGCCCTCCCGGCCGGCTCCGTGCTGGCCCATGGCGACGTCACGCCCCAGGCCGTCGATACCAAAGCGTTACCACAACTGGGCGACGAATGGCGCCCGGACAATCCCTgtctcttatacacatctccgagcccacgagacgg > GWNJ‑0478:712:GW2002102894th:2:2201:10122:99607/3‑115 (MQ=60)
tcCCTGACCGCCCTCCCGGCCGGCTCCGTGCTGGCCCATGGCGACGTCACGCCCCAGGCCGTCGATACCAAAGCGTTACCACAACTGGGCGACGAATGGCGCCCGGACAATCCCTACCGTAGCGGTGATGCGCACAAGGAGGCTCTGCGG > GWNJ‑0478:712:GW2002102894th:2:1213:21084:77414/3‑150 (MQ=60)
|
GCTTTCAGGCGCCTCCGGGCCTGGTCCGGCTGGCTGGTATGGCTCTTGCGGAAACAGGGCAAACAACCAAGACCCGTCCACTGTAGGAGACATCCATGAAAACGCAGCGCCGCCTGCTTGCCTTGCTCGCGCTTTGCGCGT‑CCTGACCGCCCTCCCGGCCGGCTCCGTGCTGGCCCATGGCGACGTCACGCCCCAGGCCGTCGATACCAAAGCGTTACCACAACTGGGCGACGAATGGCGCCCGGACAATCCCTACCGTAGCGGTGATGCGCACAAGGAGGCTCTGCGG > AM260480/1192420‑1192708
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
